Developmental biology

  • Article | | open

    Mutations that modulate the activity of ion channels are essential tools to understand the biophysical determinants that control their gating. Here authors reveal the role played by a single residue in the second transmembrane domain of vertebrate and invertebrate two-pore domain potassium channels.

    • Ismail Ben Soussia
    • , Sonia El Mouridi
    • , Dawon Kang
    • , Alice Leclercq-Blondel
    • , Lamyaa Khoubza
    • , Philippe Tardy
    • , Nora Zariohi
    • , Marie Gendrel
    • , Florian Lesage
    • , Eun-Jin Kim
    • , Delphine Bichet
    • , Olga Andrini
    •  & Thomas Boulin
  • Article | | open

    Meiotic crossover (CO) landscape differs inter- and intra-species, as well as between sexes. Here, the authors show that male meiosis produces more COs than female in maize and detect CO maturation inefficiency in some genetic backgrounds, which may help to improve breeding efficiency.

    • Cheng Luo
    • , Xiang Li
    • , Qinghua Zhang
    •  & Jianbing Yan
  • Article | | open

    Serine/threonine phosphatases such as PP1 associate with a large array of subunit proteins, such as ASPP (apoptosis-stimulating protein of p53) to achieve selective targeting. Here authors solved the crystal structure of the human ASPP2/PP1 complex and explain how ASPP2 can distinguish between PP1 isoforms.

    • M. Teresa Bertran
    • , Stéphane Mouilleron
    • , Yanxiang Zhou
    • , Rakhi Bajaj
    • , Federico Uliana
    • , Ganesan Senthil Kumar
    • , Audrey van Drogen
    • , Rebecca Lee
    • , Jennifer J. Banerjee
    • , Simon Hauri
    • , Nicola O’Reilly
    • , Matthias Gstaiger
    • , Rebecca Page
    • , Wolfgang Peti
    •  & Nicolas Tapon
  • Article | | open

    Calcium signalling downstream of VEGF is essential for VEGF-induced angiogenesis. Here Savage et al. show that Transmembrane Protein 33 (TMEM33) is required for angiogenesis and the endothelial calcium response to VEGF, revealing a function for TMEM33 in multicellular organisms.

    • Aaron M. Savage
    • , Sathishkumar Kurusamy
    • , Yan Chen
    • , Zhen Jiang
    • , Karishma Chhabria
    • , Ryan B. MacDonald
    • , Hyejeong R. Kim
    • , Heather L. Wilson
    • , Fredericus J. M. van Eeden
    • , Angel L. Armesilla
    • , Timothy J. A. Chico
    •  & Robert N. Wilkinson
  • Article | | open

    Whether the Wnt enhanceosome’ components BCL9/9l can affect intestinal homeostasis and tumorigenesis is still unclear. Using conditional Bcl9/9l KO mice, the authors of this study show that the BCL9/9l complex is required for intestinal stem cells to drive tissue regeneration and that loss of BCL9/9l suppresses Wnt-driven transformation.

    • David M. Gay
    • , Rachel A. Ridgway
    • , Miryam Müeller
    • , Michael C. Hodder
    • , Ann Hedley
    • , William Clark
    • , Joshua D. Leach
    • , Rene Jackstadt
    • , Colin Nixon
    • , David J. Huels
    • , Andrew D. Campbell
    • , Thomas G. Bird
    •  & Owen J. Sansom
  • Article | | open

    BCL9 and Pygo are components of Wnt enhanceosome, which facilitates β-catenin-dependent transcription. Here, the authors show that deletion of Bcl9 and Pygo suppresses tumorigenesis and extends disease free survival in two different colorectal cancer models, suggesting a strategy for drugging β-catenin signalling in this cancer.

    • Juliusz Mieszczanek
    • , Laurens M. van Tienen
    • , Ashraf E. K. Ibrahim
    • , Douglas J. Winton
    •  & Mariann Bienz
  • Article | | open

    Auxin transport and microtubule orientation respond to mechanical stimulation at the shoot apical meristem. Here Li et al. show that mechanical stimulation causes cytosolic calcium concentration transients, and preventing such changes impairs reorientation of the PIN1 auxin efflux carrier, but not of microtubules.

    • Ting Li
    • , An Yan
    • , Neha Bhatia
    • , Alphan Altinok
    • , Eldad Afik
    • , Pauline Durand-Smet
    • , Paul T. Tarr
    • , Julian I. Schroeder
    • , Marcus G. Heisler
    •  & Elliot M. Meyerowitz
  • Article | | open

    Transcription is globally repressed in early stage of embryo development, but a set of genes including pri-miR-430 and zinc finger genes is known to escape the repression. Here the authors image the very first transcriptional activities in the living zebra fish embryo, demonstrating a cell cycle-coordinated polymerase II transcription compartment.

    • Yavor Hadzhiev
    • , Haseeb K. Qureshi
    • , Lucy Wheatley
    • , Ledean Cooper
    • , Aleksandra Jasiulewicz
    • , Huy Van Nguyen
    • , Joseph W. Wragg
    • , Divyasree Poovathumkadavil
    • , Sascha Conic
    • , Sarah Bajan
    • , Attila Sik
    • , György Hutvàgner
    • , Làszlò Tora
    • , Agnieszka Gambus
    • , John S. Fossey
    •  & Ferenc Müller
  • Article | | open

    Programmed cell death regulates early development but how various factors (for example, TRADD, FADD and RIPKs) regulate this is unclear. Here, the authors show that a single allele of Tradd is essential for survival, when both Ripk3 and Ripk1 are knocked out in mice, and RIPK1 protects thymocytes from TNF-induced apoptosis.

    • John P. Dowling
    • , Mohamed Alsabbagh
    • , Christina Del Casale
    • , Zheng-Gang Liu
    •  & Jianke Zhang
  • Article | | open

    The diversity of fibroblasts contributing to wound healing is unclear. Here, the authors use single-cell RNA-sequencing to identify heterogeneity among murine fibroblasts in the wound and find that recruited myeloid cells contribute to adipocyte regeneration during healing.

    • Christian F. Guerrero-Juarez
    • , Priya H. Dedhia
    • , Suoqin Jin
    • , Rolando Ruiz-Vega
    • , Dennis Ma
    • , Yuchen Liu
    • , Kosuke Yamaga
    • , Olga Shestova
    • , Denise L. Gay
    • , Zaixin Yang
    • , Kai Kessenbrock
    • , Qing Nie
    • , Warren S. Pear
    • , George Cotsarelis
    •  & Maksim V. Plikus
  • Article | | open

    BMP ligands act as retrograde signalling molecules to regulate presynaptic development, and regulation of BMP receptors by endocytosis may be an important component of this signalling pathway. Here, the authors show that Abi-mediated macropinocytosis of BMP receptors in Drosophila larva and contributes to neuromuscular development.

    • Najin Kim
    • , Sungdae Kim
    • , Minyeop Nahm
    • , Danielle Kopke
    • , Joohyung Kim
    • , Eunsang Cho
    • , Min-Jung Lee
    • , Mihye Lee
    • , Seung Hyun Kim
    • , Kendal Broadie
    •  & Seungbok Lee
  • Article | | open

    The pathways involved in exit from pluripotency in human embryonic stem cells are poorly understood. Here, the authors performed a CRISPR-based screen to identify genes that promote exit from naïve pluripotency and find a role for folliculin (FLCN) by regulating the mTOR and Wnt pathways.

    • J. Mathieu
    • , D. Detraux
    • , D. Kuppers
    • , Y. Wang
    • , C. Cavanaugh
    • , S. Sidhu
    • , S. Levy
    • , A. M. Robitaille
    • , A. Ferreccio
    • , T. Bottorff
    • , A. McAlister
    • , L. Somasundaram
    • , F. Artoni
    • , S. Battle
    • , R. D. Hawkins
    • , R. T. Moon
    • , C. B. Ware
    • , P. J. Paddison
    •  & H. Ruohola-Baker
  • Article | | open

    Hematopoietic stem cell (HSC) transplantation is a desirable treatment for many non-malignant and malignant diseases, but its use requires preconditioning of recipients with irradiation or chemotherapy that often induces high toxicity. Here the authors show that antibody-drug-conjugate to CD117, a HSC marker, allows specific and efficient preconditioning for HSC therapy.

    • Agnieszka Czechowicz
    • , Rahul Palchaudhuri
    • , Amelia Scheck
    • , Yu Hu
    • , Jonathan Hoggatt
    • , Borja Saez
    • , Wendy W. Pang
    • , Michael K. Mansour
    • , Tiffany A. Tate
    • , Yan Yi Chan
    • , Emily Walck
    • , Gerlinde Wernig
    • , Judith A. Shizuru
    • , Florian Winau
    • , David T. Scadden
    •  & Derrick J. Rossi
  • Article | | open

    Transplantation of allogeneic bone marrow helps establish chimerism that may induce tolerance to tissue grafts. Here the authors show that a CD117-antibody-drug-conjugate helps precondition the recipients for inducing mixed chimerism and allo-tolerance without clear adverse effects or the need for chronic immune suppression.

    • Zhanzhuo Li
    • , Agnieszka Czechowicz
    • , Amelia Scheck
    • , Derrick J. Rossi
    •  & Philip M. Murphy
  • Article | | open

    The use of pluripotent-stem cell derived organs for transplantation would be promising, if organs can be grown in a suitable host. Here, the authors use interspecific blastocyst complementation to generate a mouse pluripotent stem cell-derived kidney in anephric Sall1 mutant rats.

    • Teppei Goto
    • , Hiromasa Hara
    • , Makoto Sanbo
    • , Hideki Masaki
    • , Hideyuki Sato
    • , Tomoyuki Yamaguchi
    • , Shinichi Hochi
    • , Toshihiro Kobayashi
    • , Hiromitsu Nakauchi
    •  & Masumi Hirabayashi
  • Article | | open

    Mammalian joints have poor regenerative capacity following amputation. Here, the authors show that in mice, stimulation of the amputation wound with BMP2 and BMP9 stimulates regeneration of a synovial joint that includes bone, cartilage and a synovial cavity.

    • Ling Yu
    • , Lindsay A. Dawson
    • , Mingquan Yan
    • , Katherine Zimmel
    • , Yu-Lieh Lin
    • , Connor P. Dolan
    • , Manjong Han
    •  & Ken Muneoka
  • Article | | open

    Midbrain dopamine (mDA) neurons are significantly associated with Parkinson’s disease and yet there is no systematic molecular classification of these heterogenous group of cells. Here authors use single cell RNA sequencing of isolated mouse neurons expressing the transcription factor Pitx3 (broad mDA neuronal marker) to identify and characterize seven neuron subgroups divided in two major branches of developing Pitx3-expressing neurons.

    • Katarína Tiklová
    • , Åsa K. Björklund
    • , Laura Lahti
    • , Alessandro Fiorenzano
    • , Sara Nolbrant
    • , Linda Gillberg
    • , Nikolaos Volakakis
    • , Chika Yokota
    • , Markus M. Hilscher
    • , Thomas Hauling
    • , Fredrik Holmström
    • , Eliza Joodmardi
    • , Mats Nilsson
    • , Malin Parmar
    •  & Thomas Perlmann
  • Article | | open

    The exon junction complex (EJC) has been identified as functioning as a post-transcriptional gene regulator. Here the authors reveal that the complex also plays a part in the regulation of transcription by regulating the release of Pol II pausing into transcription elongation.

    • Junaid Akhtar
    • , Nastasja Kreim
    • , Federico Marini
    • , Giriram Mohana
    • , Daniel Brüne
    • , Harald Binder
    •  & Jean-Yves Roignant
  • Article | | open

    High fat diets (HFD) are thought to perturb murine hematopoiesis as a result of obesity. Here the authors find that short-term HFD reduces hematopoietic stem cells (HSC), disrupts lipid rafts and TGF-β1 signalling. Injecting HFD-fed mice with recombinant TGF-β1 can rescue HSC loss.

    • François Hermetet
    • , Anne Buffière
    • , Aziza Aznague
    • , Jean-Paul Pais de Barros
    • , Jean-Noël Bastie
    • , Laurent Delva
    •  & Ronan Quéré
  • Article | | open

    Lineage segregation from conception to gastrulation has been mapped at the single cell level in mouse, human and monkey. Here, the authors provide a comprehensive analysis of porcine preimplantation development using single cell RNA-seq; mapping metabolic changes, X chromosome inactivation and signalling pathways.

    • Priscila Ramos-Ibeas
    • , Fei Sang
    • , Qifan Zhu
    • , Walfred W. C. Tang
    • , Sarah Withey
    • , Doris Klisch
    • , Liam Wood
    • , Matt Loose
    • , M. Azim Surani
    •  & Ramiro Alberio
  • Article | | open

    The precise cellular patterning and decisions of early embryogenesis have been hard to mimic in vitro. Here, the authors culture murine embryonic and trophoblast stem cells together with extra-embryonic endoderm stem cells to form embryo-like structures (ETX-embryoids), which can initiate an implantation response.

    • Shaopeng Zhang
    • , Tianzhi Chen
    • , Naixin Chen
    • , Dengfeng Gao
    • , Bingbo Shi
    • , Shuangbo Kong
    • , Rachel Claire West
    • , Ye Yuan
    • , Minglei Zhi
    • , Qingqing Wei
    • , Jinzhu Xiang
    • , Haiyuan Mu
    • , Liang Yue
    • , Xiaohua Lei
    • , Xuepeng Wang
    • , Liang Zhong
    • , Hui Liang
    • , Suying Cao
    • , Juan Carlos Izpisua Belmonte
    • , Haibin Wang
    •  & Jianyong Han
  • Article | | open

    The establishment of functional vasculatures requires the specification of newly formed vessels into veins and arteries. Here, Neal et al. use a combination of genetic approaches in mice and zebrafish to show that BMP signalling, via ALK3 and SMAD1/5, is required for venous specification during blood vessel development.

    • Alice Neal
    • , Svanhild Nornes
    • , Sophie Payne
    • , Marsha D. Wallace
    • , Martin Fritzsche
    • , Pakavarin Louphrasitthiphol
    • , Robert N. Wilkinson
    • , Kira M. Chouliaras
    • , Ke Liu
    • , Karen Plant
    • , Radhika Sholapurkar
    • , Indrika Ratnayaka
    • , Wiebke Herzog
    • , Gareth Bond
    • , Tim Chico
    • , George Bou-Gharios
    •  & Sarah De Val
  • Article | | open

    Hippo signaling leads to the phosphorylation of the key transcriptional effector, Yap/Yki, although how Yap/Yki stability is regulated has remained unclear. Here, Sun et al. identify HAUSP/Usp7 as a conserved and clinically relevant regulator of the Hippo pathway that increases Yap/Yki stability.

    • Xiaohan Sun
    • , Yan Ding
    • , Meixiao Zhan
    • , Yan Li
    • , Dongqing Gao
    • , Guiping Wang
    • , Yang Gao
    • , Yong Li
    • , Shian Wu
    • , Ligong Lu
    • , Qingxin Liu
    •  & Zizhang Zhou
  • Article | | open

    Glycogen Synthase Kinase 3 (GSK3) has conserved functions across species but its role in the kidney is unclear. Here, the authors show that in the kidney podocyte cell of mice and related nephrocyte in Drosophila that GSK3 is a master regulator of function both during development and in maturity.

    • J. A. Hurcombe
    • , P. Hartley
    • , A. C. Lay
    • , L. Ni
    • , J. J. Bedford
    • , J. P. Leader
    • , S. Singh
    • , A. Murphy
    • , C. L. Scudamore
    • , E. Marquez
    • , A. F. Barrington
    • , V. Pinto
    • , M. Marchetti
    • , L.-F. Wong
    • , J. Uney
    • , M. A. Saleem
    • , P. W. Mathieson
    • , S. Patel
    • , R. J. Walker
    • , J. R. Woodgett
    • , S. E. Quaggin
    • , G. I. Welsh
    •  & R. J. M. Coward
  • Article | | open

    Cortical microtubules of Toxoplasma gondii are exceptionally stable, but it isn’t known how they are anchored along membranes. Here, Harding et al. show that GAPM proteins localize to the inner membrane complex and are essential for maintaining the structural stability of parasites.

    • Clare R. Harding
    • , Matthew Gow
    • , Joon Ho Kang
    • , Emily Shortt
    • , Scott R. Manalis
    • , Markus Meissner
    •  & Sebastian Lourido
  • Article | | open

    Enhanced Wnt receptor activity is a major cause of cancer development. Here the authors identify camelid single-domain antibody fragments (VHHs) that bind to the Wnt receptor LRP5/6 ectodomain, determine the crystal structures and show that these VHHs selectively inhibit Wnt3- mediated cellular responses and block the growth of mutant Wnt-hypersensitive intestinal tumor organoids.

    • Nicola Fenderico
    • , Revina C. van Scherpenzeel
    • , Michael Goldflam
    • , Davide Proverbio
    • , Ingrid Jordens
    • , Tomica Kralj
    • , Sarah Stryeck
    • , Tarek Z. Bass
    • , Guy Hermans
    • , Christopher Ullman
    • , Teodor Aastrup
    • , Piet Gros
    •  & Madelon M. Maurice
  • Article | | open

    Myelodyplastic hematopoietic stem cells (MDS HSC) have eluded in vivo modeling. Here the authors present a highly efficient MDS patient-derived xenotransplantation model in cytokine-humanized mice with replication of the donors’ genetic complexity and myeloid, erythroid, and megakaryocytic lineage dysplasia.

    • Yuanbin Song
    • , Anthony Rongvaux
    • , Ashley Taylor
    • , Tingting Jiang
    • , Toma Tebaldi
    • , Kunthavai Balasubramanian
    • , Arun Bagale
    • , Yunus Kasim Terzi
    • , Rana Gbyli
    • , Xiaman Wang
    • , Xiaoying Fu
    • , Yimeng Gao
    • , Jun Zhao
    • , Nikolai Podoltsev
    • , Mina Xu
    • , Natalia Neparidze
    • , Ellice Wong
    • , Richard Torres
    • , Emanuela M. Bruscia
    • , Yuval Kluger
    • , Markus G. Manz
    • , Richard A. Flavell
    •  & Stephanie Halene
  • Article | | open

    Early human embryonic development involves extensive changes in chromatin structure and transcriptional activity. Here the authors present LiCAT-seq, a method enabling simultaneous profiling of chromatin accessibility and gene expression with ultra-low input of cells and map chromatin accessibility and transcriptome landscapes for human pre-implantation embryos.

    • Longqi Liu
    • , Lizhi Leng
    • , Chuanyu Liu
    • , Changfu Lu
    • , Yue Yuan
    • , Liang Wu
    • , Fei Gong
    • , Shuoping Zhang
    • , Xiaoyu Wei
    • , Mingyue Wang
    • , Lei Zhao
    • , Liang Hu
    • , Jian Wang
    • , Huanming Yang
    • , Shida Zhu
    • , Fang Chen
    • , Guangxiu Lu
    • , Zhouchun Shang
    •  & Ge Lin
  • Article | | open

    Hydra regenerate various body parts on amputation by activation of the appropriate organiser, but how head formation is controlled is unclear. Here, the authors identify the transcription factor Sp5 as restricting head formation, by being activated by beta-catenin and then acting as a repressor of Wnt3.

    • Matthias C. Vogg
    • , Leonardo Beccari
    • , Laura Iglesias Ollé
    • , Christine Rampon
    • , Sophie Vriz
    • , Chrystelle Perruchoud
    • , Yvan Wenger
    •  & Brigitte Galliot
  • Article | | open

    Tissue-resident macrophages are highly specialized phagocytes that serve multiple functions. Here, using high-dimension analyses and fate-mapping experiments, the authors show that fetal liver-derived macrophages dominate the mammary gland in neonatal and adult, and display characteristic phenotypes and functions.

    • Norma Jäppinen
    • , Inês Félix
    • , Emmi Lokka
    • , Sofia Tyystjärvi
    • , Anne Pynttäri
    • , Tiina Lahtela
    • , Heidi Gerke
    • , Kati Elima
    • , Pia Rantakari
    •  & Marko Salmi
  • Article | | open

    Neural tube defects can arise from high glucose levels caused by maternal diabetes, and MARCKS is required for neural tube closure. Here, Yang et al. show that acetylation and phosphorylation of MARCKS in hyperglycemic conditions causes mitochondrial and ER stress, leading to neural tube defects.

    • Penghua Yang
    • , Cheng Xu
    • , E. Albert Reece
    • , Xi Chen
    • , Jianxiang Zhong
    • , Min Zhan
    • , Deborah J. Stumpo
    • , Perry J. Blackshear
    •  & Peixin Yang
  • Article | | open

    Lymphangiogenesis occurs in the context of systemic inflammation and development but has not been reported for the lymphatics that surround the CNS. Here the authors show that in the context of experimental autoimmune encephatlitis, lymphangiogenesis occurs at the cribriform plate, but not the meninges, and contributes to immune cell and antigen drainage.

    • Martin Hsu
    • , Aditya Rayasam
    • , Julie A. Kijak
    • , Yun Hwa Choi
    • , Jeffrey S. Harding
    • , Sarah A. Marcus
    • , William J. Karpus
    • , Matyas Sandor
    •  & Zsuzsanna Fabry
  • Article | | open

    Many organs develop through branching morphogenesis, but whether the underlying mechanisms are shared is unknown. Here, the authors show that a ligand-receptor based Turing mechanisms, similar to that observed in lung development, likely underlies branching morphogenesis of the kidney.

    • Denis Menshykau
    • , Odyssé Michos
    • , Christine Lang
    • , Lisa Conrad
    • , Andrew P. McMahon
    •  & Dagmar Iber
  • Article | | open

    Animal studies have shown that the nutritional status of parents can predispose the offspring to obesity and obesity-related diseases. Here the authors show that cardiac dysfunction induced by a high-fat diet persists for two generations in Drosophila, and that targeted expression of ATGL/bmm in the offspring, as well as inhibition of H3K27 trimethylation, is cardioprotective.

    • Maria Clara Guida
    • , Ryan Tyge Birse
    • , Alessandra Dall’Agnese
    • , Paula Coutinho Toto
    • , Soda Balla Diop
    • , Antonello Mai
    • , Peter D. Adams
    • , Pier Lorenzo Puri
    •  & Rolf Bodmer
  • Article | | open

    Nephrogenesis ceases after postnatal day 2 in the mouse or after the 36th week of gestation in humans, but how this is regulated is unclear. Here, the authors identify a role for the RNA-binding protein Lin28 and suppression of let-7 microRNA in regulating the duration of nephrogenesis.

    • Alena V. Yermalovich
    • , Jihan K. Osborne
    • , Patricia Sousa
    • , Areum Han
    • , Melissa A. Kinney
    • , Michael J. Chen
    • , Daisy A. Robinton
    • , Helen Montie
    • , Dan S. Pearson
    • , Sean B. Wilson
    • , Alexander N. Combes
    • , Melissa H. Little
    •  & George Q. Daley
  • Article | | open

    It is unclear how some Drosophila neurons develop sex-specific neurites that contribute to dimorphic circuitries required for gendered behavior. The authors show that sex-specific cleavage by the E3 ubiquitin ligase Cullin1 and 26S proteasome of the pleiotropic BTB-ZF transcription factor Lola confers its sexual fate-inducing ability in these neurons.

    • Kosei Sato
    • , Hiroki Ito
    • , Atsushi Yokoyama
    • , Gakuta Toba
    •  & Daisuke Yamamoto
  • Article | | open

    Most tumours are characterized by increased aerobic glycolytic activity. Here the authors show that elevated  aerobic glycolysis is not essential for cancer initiation by testing the effect of lactate dehydrogenase depletion on the ability of hair follicle stem cells (HFSCs) to form squamous cell carcinoma (SCC) in mouse genetic models.

    • A. Flores
    • , S. Sandoval-Gonzalez
    • , R. Takahashi
    • , A. Krall
    • , L. Sathe
    • , L. Wei
    • , C. Radu
    • , J. Jolly
    • , N. Graham
    • , H. R. Christofk
    •  & W. E. Lowry
  • Article | | open

    While human lifespan is only moderately heritable, “getting old” runs in families. Here, van den Berg et al. study mortality data from three-generation cohorts to define a threshold for longevity and find that individuals have an increasing survival advantage with each additional relative in the top 10% survivors of their birth cohort.

    • Niels van den Berg
    • , Mar Rodríguez-Girondo
    • , Ingrid K. van Dijk
    • , Rick J. Mourits
    • , Kees Mandemakers
    • , Angelique A. P. O. Janssens
    • , Marian Beekman
    • , Ken R. Smith
    •  & P. Eline Slagboom
  • Article | | open

    In Ciona, cardiopharyngeal progenitors migrate as polarized pairs of cells between the epidermis and the endoderm. Here the authors show that the endoderm secretes a particular collagen, which promotes Discoidin domain receptor activity to control cell-matrix adhesion and collective polarity.

    • Yelena Y. Bernadskaya
    • , Saahil Brahmbhatt
    • , Stephanie E. Gline
    • , Wei Wang
    •  & Lionel Christiaen
  • Article | | open

    Transposable elements (TEs) fulfill essential but poorly understood roles in genome organization and gene expression control. Here the authors show that the regulation of TEs occurs through overlapping epigenetic mechanisms that control the expression and chromatin signatures at TEs.

    • Jiangping He
    • , Xiuling Fu
    • , Meng Zhang
    • , Fangfang He
    • , Wenjuan Li
    • , Mazid Md. Abdul
    • , Jianguo Zhou
    • , Li Sun
    • , Chen Chang
    • , Yuhao Li
    • , He Liu
    • , Kaixin Wu
    • , Isaac A. Babarinde
    • , Qiang Zhuang
    • , Yuin-Han Loh
    • , Jiekai Chen
    • , Miguel A. Esteban
    •  & Andrew P. Hutchins
  • Article | | open

    The inactive X chromosome (Xi) has an atypical structure, with global loss of TADs, A/B compartments and formation of mega-domains. Here the authors show that the non-canonical SMC family protein, SmcHD1, important for developmental gene silencing on Xi, antagonises TAD formation and compartmentalization on the Xi in a transcription independent way.

    • Michal R. Gdula
    • , Tatyana B. Nesterova
    • , Greta Pintacuda
    • , Jonathan Godwin
    • , Ye Zhan
    • , Hakan Ozadam
    • , Michael McClellan
    • , Daniella Moralli
    • , Felix Krueger
    • , Catherine M. Green
    • , Wolf Reik
    • , Skirmantas Kriaucionis
    • , Edith Heard
    • , Job Dekker
    •  & Neil Brockdorff
  • Article | | open

    The PI3K/Akt/mTOR pathway has been previously implicated in fibrosis and a pan-PI3K/mTOR inhibitor is currently under clinical evaluation for the treatment of IPF. Here the authors show that the mTORC1/4E-BP1 axis is critical for TGF-β1-induced fibrogenesis in in vitro and ex vivo models and that canonical PI3K/Akt signalling is dispensable.

    • Hannah V. Woodcock
    • , Jessica D. Eley
    • , Delphine Guillotin
    • , Manuela Platé
    • , Carmel B. Nanthakumar
    • , Matteo Martufi
    • , Simon Peace
    • , Gerard Joberty
    • , Daniel Poeckel
    • , Robert B. Good
    • , Adam R. Taylor
    • , Nico Zinn
    • , Matthew Redding
    • , Ellen J. Forty
    • , Robert E. Hynds
    • , Charles Swanton
    • , Morten Karsdal
    • , Toby M. Maher
    • , Giovanna Bergamini
    • , Richard P. Marshall
    • , Andy D. Blanchard
    • , Paul F. Mercer
    •  & Rachel C. Chambers
  • Article | | open

    A decrease in the fraction of non-classical monocytes is a hallmark of chronic myelomonocytic leukaemia. Taking advantage of this abnormal situation, the authors identify a mechanistic link between miR-150 and TET3 as being involved in monocyte subset generation.

    • Dorothée Selimoglu-Buet
    • , Julie Rivière
    • , Hussein Ghamlouch
    • , Laura Bencheikh
    • , Catherine Lacout
    • , Margot Morabito
    • , M’boyba Diop
    • , Guillaume Meurice
    • , Marie Breckler
    • , Aurélie Chauveau
    • , Camille Debord
    • , Franck Debeurme
    • , Raphael Itzykson
    • , Nicolas Chapuis
    • , Christophe Willekens
    • , Orianne Wagner-Ballon
    • , Olivier A. Bernard
    • , Nathalie Droin
    •  & Eric Solary
  • Article | | open

    Senescent cells accumulate with aging contributing to age-related disease and the role of the immune system in removing senescent cells is not completely understood. Here, the authors show that perforin deficient mice accumulate more senescent cells and have a shorter lifespan, and that this phenotype can be reversed with administration of a senolytic drug.

    • Yossi Ovadya
    • , Tomer Landsberger
    • , Hanna Leins
    • , Ezra Vadai
    • , Hilah Gal
    • , Anat Biran
    • , Reut Yosef
    • , Adi Sagiv
    • , Amit Agrawal
    • , Alon Shapira
    • , Joseph Windheim
    • , Michael Tsoory
    • , Reinhold Schirmbeck
    • , Ido Amit
    • , Hartmut Geiger
    •  & Valery Krizhanovsky
  • Article | | open

    The source of where definitive hematopoietic stem cells (dHSCs) develop in the mouse embyro has been much debated. Here, the authors identify that dHSCs arise from the intra-embyronic region by using murine embryonic explant transplantation but not from the yolk sac.

    • Miguel Ganuza
    • , Ashley Chabot
    • , Xing Tang
    • , Wenjian Bi
    • , Sivaraman Natarajan
    • , Robert Carter
    • , Charles Gawad
    • , Guolian Kang
    • , Yong Cheng
    •  & Shannon McKinney-Freeman
  • Article | | open

    The molecular mechanisms regulating mesenchymal cell movements are unclear. Here, the authors show in chicken feather elongation that SHH/WNT signalling establishes gap-junctions, enabling synchronized Ca2 + oscillations to emerge for and in turn coordinating directed cell migration.

    • Ang Li
    • , Jung-Hwa Cho
    • , Brian Reid
    • , Chun-Chih Tseng
    • , Lian He
    • , Peng Tan
    • , Chao-Yuan Yeh
    • , Ping Wu
    • , Yuwei Li
    • , Randall B. Widelitz
    • , Yubin Zhou
    • , Min Zhao
    • , Robert H. Chow
    •  & Cheng-Ming Chuong
  • Article | | open

    Mechanisms that operate during embryonic development to restrict cell fate are currently under investigation. Here the authors characterise the role of SCL/TAL1 at the onset of blood specification in embryonic development using mouse EB differentiation culture as a model system.

    • Hedia Chagraoui
    • , Maiken S. Kristiansen
    • , Juan Pablo Ruiz
    • , Ana Serra-Barros
    • , Johanna Richter
    • , Elisa Hall-Ponselé
    • , Nicki Gray
    • , Dominic Waithe
    • , Kevin Clark
    • , Philip Hublitz
    • , Emmanouela Repapi
    • , Georg Otto
    • , Paul Sopp
    • , Stephen Taylor
    • , Supat Thongjuea
    • , Paresh Vyas
    •  & Catherine Porcher
  • Article | | open

    Human embryonic stem cells can be differentiated in vitro into primordial germ cell-like cells (PGCLCs) that resemble early primordial germ cells (PGCs). Here the authors transplant PGCLCs generated from rhesus macaque iPSCs into mouse and rhesus macaque seminiferous tubules, which matures these into late PGCs and spermatogonia-like cells.

    • Enrique Sosa
    • , Di Chen
    • , Ernesto J. Rojas
    • , Jon D. Hennebold
    • , Karen A. Peters
    • , Zhuang Wu
    • , Truong N. Lam
    • , Jennifer M. Mitchell
    • , Meena Sukhwani
    • , Ramesh C. Tailor
    • , Marvin L. Meistrich
    • , Kyle E. Orwig
    • , Gunapala Shetty
    •  & Amander T. Clark