Research Highlight |
Featured
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Research Highlight |
A body-wide view of somatic mutations
Four new studies in Nature report multi-tissue analyses of somatic mutations from human donors, with insights into cell lineage commitment during embryonic development, as well as tissue-specific aspects of mutagenesis.
- Darren J. Burgess
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Research Highlight |
Human cell-lineage imbalances
Two new studies in Science use lineage tracking in humans to show that among the first few embryonic cells originating from post-fertilization cell divisions, there can be substantial imbalance in their contributions to mature tissues.
- Darren J. Burgess
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Research Highlight |
Host genetics of coronavirus infection
Two new reports in Cell use genome-wide CRISPR screens to uncover host determinants of coronavirus infection, identifying potential leads for antiviral therapeutics.
- Darren J. Burgess
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Research Highlight |
A mouse with history
A new study in Cell describes the CRISPR array repair lineage tracing (CARLIN) engineered mouse line that genomically encodes all the components for CRISPR-based lineage tracking at single-cell resolution.
- Darren J. Burgess
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Review Article |
Lineage tracing meets single-cell omics: opportunities and challenges
Understanding developmental trajectories has recently been enabled by progress in modern lineage-tracing methods that combine genetic lineage analysis with omics-based characterization of cell states (particularly transcriptomes). In this Review, Wagner and Klein discuss the conceptual underpinnings, experimental strategies and analytical considerations of these approaches, as well as the biological insights gained.
- Daniel E. Wagner
- & Allon M. Klein
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Research Highlight |
Transcriptomics of developmental fate
Two new studies in Science combine single-cell RNA sequencing with either lineage tracing or a computational framework to link transcriptomes to future developmental trajectories.
- Darren J. Burgess
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Research Highlight |
CRISPR screens come into sight
A new study in Cell reports a mammalian genetic screening strategy that combines CRISPR libraries with in situ sequencing to read out both complex cellular phenotypes and genetic perturbations using microscopy.
- Darren J. Burgess
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Research Highlight |
Genotype–phenotype mapping in another dimension
A study in Science shows that it is now possible to create high-resolution, non-linear maps of mammalian genetic interactions.
- Linda Koch
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Review Article |
DNA-based memory devices for recording cellular events
In this Review, Sheth and Wang describe emerging synthetic biology approaches for using DNA as a memory device for recording cellular events, including the various methodological steps from detecting diverse signals, converting them into DNA alterations and reading out and interpreting the recorded information. Furthermore, they discuss potential applications as biotechnological and environmental biosensors.
- Ravi U. Sheth
- & Harris H. Wang
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Research Highlight |
Prospecting for pluripotency
Unlike most organisms, planaria maintain pluripotent stem cells (PSCs) beyond embryogenesis. A new study reports the prospective identification and isolation of a neoblast subpopulation containing adult PSCs, characterization of which should help uncover the mechanisms underlying pluripotency and tissue regeneration.
- Dorothy Clyde
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Research Highlight |
Tracing cell-lineage histories
Three new studies in Nature and Nature Biotechnology report methods for dissecting transcriptomic cell phenotypes and lineage history simultaneously by combining single-cell RNA sequencing (scRNA-seq) with CRISPR-based lineage tracing.
- Darren J. Burgess
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A space for transcriptomics
Genome editing for cell lineage tracing
DNA sequencing trumps standard screening tools