CRISPR-Cas systems articles within Nature Communications

Featured

• Article
  17 August 2016 | Open Access

  Immunogenomic engineering of a plug-and-(dis)play hybridoma platform

  Hybridomas are widely used for antibody screening and production due to their genetic stability and rapid proliferation. Here the authors demonstrate the rapid reprogramming of antibody specificity in hybridomas using CRISPR-Cas9.

  • Mark Pogson
  • , Cristina Parola
  •  & Sai T. Reddy

• Article
  10 August 2016 | Open Access

  Writing of H3K4Me3 overcomes epigenetic silencing in a sustained but context-dependent manner

  Epigenome editing by zinc finger (ZF) and CRISPR-dCas9 technologies can induce or repress gene expression. Here, the authors show that histone methyltransferase PRDM9 fused to either dCas9 or ZF proteins can sustain gene re-expression, and H3K79me is required for stable gene re-expression.

  • David Cano-Rodriguez
  • , Rutger A F. Gjaltema
  •  & Marianne G Rots

• Article
  28 July 2016 | Open Access

  CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism

  The use of site-specific gene insertion is a powerful method for investigating gene function. Here the authors describe CRISPaint, a universal tagging system using CRISPR-Cas9 to insert genes in an NHEJ dependent manner.

  • Jonathan L. Schmid-Burgk
  • , Klara Höning
  •  & Veit Hornung