Chemical genetics

Chemical genetics is the use of small molecule compounds to perturb a biological system to explore the outcome. Chemical genetics is also used to describe the technique of screening for small molecule modulators. As in classical genetics, the perturbation of a system using small molecules reveals information about the targeted system.

Latest Research and Reviews

  • Research | | open

    Core regulatory transcription factors are usually regulated by cell-type specific super enhancers (SEs). Here, the authors screen for chemical probes able to distinguish between SE-driven and promoter-driven transcription and find that histone deacetylases are selectively required for core regulatory transcription.

    • Berkley E. Gryder
    • , Lei Wu
    • , Girma M. Woldemichael
    • , Silvia Pomella
    • , Taylor R. Quinn
    • , Paul M. C. Park
    • , Abigail Cleveland
    • , Benjamin Z. Stanton
    • , Young Song
    • , Rossella Rota
    • , Olaf Wiest
    • , Marielle E. Yohe
    • , Jack F. Shern
    • , Jun Qi
    •  & Javed Khan
  • Research |

    A high-throughput chemical–genetic screening approach for the discovery of targets and chemicals to treat Mycobacterium tuberculosis yields tenfold more hit compounds than conventional whole-cell screening methods.

    • Eachan O. Johnson
    • , Emily LaVerriere
    • , Emma Office
    • , Mary Stanley
    • , Elisabeth Meyer
    • , Tomohiko Kawate
    • , James E. Gomez
    • , Rebecca E. Audette
    • , Nirmalya Bandyopadhyay
    • , Natalia Betancourt
    • , Kayla Delano
    • , Israel Da Silva
    • , Joshua Davis
    • , Christina Gallo
    • , Michelle Gardner
    • , Aaron J. Golas
    • , Kristine M. Guinn
    • , Sofia Kennedy
    • , Rebecca Korn
    • , Jennifer A. McConnell
    • , Caitlin E. Moss
    • , Kenan C. Murphy
    • , Raymond M. Nietupski
    • , Kadamba G. Papavinasasundaram
    • , Jessica T. Pinkham
    • , Paula A. Pino
    • , Megan K. Proulx
    • , Nadine Ruecker
    • , Naomi Song
    • , Matthew Thompson
    • , Carolina Trujillo
    • , Shoko Wakabayashi
    • , Joshua B. Wallach
    • , Christopher Watson
    • , Thomas R. Ioerger
    • , Eric S. Lander
    • , Brian K. Hubbard
    • , Michael H. Serrano-Wu
    • , Sabine Ehrt
    • , Michael Fitzgerald
    • , Eric J. Rubin
    • , Christopher M. Sassetti
    • , Dirk Schnappinger
    •  & Deborah T. Hung
    Nature 571, 72-78
  • Research |

    The solid media portable cell killing assay uses metabolism-sensitive staining to illuminate the killing of antibiotic-tolerant bacteria under resource-depleted conditions, thereby enabling multiplex, genome-scale analyses for the identification of target strains.

    • Jonathan M. Stokes
    • , Arnaud Gutierrez
    • , Allison J. Lopatkin
    • , Ian W. Andrews
    • , Shawn French
    • , Ivan Matic
    • , Eric D. Brown
    •  & James J. Collins
    Nature Methods 16, 303-306
  • Research | | open

    Premature termination codon suppression therapy could be used to treat a range of genetic disorders. Here the authors present a high-throughput cell-based assay to identify anticodon engineered tRNAs with high suppression activity.

    • John D. Lueck
    • , Jae Seok Yoon
    • , Alfredo Perales-Puchalt
    • , Adam L. Mackey
    • , Daniel T. Infield
    • , Mark A. Behlke
    • , Marshall R. Pope
    • , David B. Weiner
    • , William R. Skach
    • , Paul B. McCray Jr.
    •  & Christopher A. Ahern

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