Biotechnology

  • Article | | open

    Simple, fast, and accurate detection of food freshness has great significance to food safety and business. Here, the authors develop cellulose-based ratiometric fluorescent materials with superior amine-response, which can be used for visual monitoring the freshness of shrimp and crab.

    • Ruonan Jia
    • , Weiguo Tian
    • , Haotian Bai
    • , Jinming Zhang
    • , Shu Wang
    •  & Jun Zhang
  • Article | | open

    Mutations that modulate the activity of ion channels are essential tools to understand the biophysical determinants that control their gating. Here authors reveal the role played by a single residue in the second transmembrane domain of vertebrate and invertebrate two-pore domain potassium channels.

    • Ismail Ben Soussia
    • , Sonia El Mouridi
    • , Dawon Kang
    • , Alice Leclercq-Blondel
    • , Lamyaa Khoubza
    • , Philippe Tardy
    • , Nora Zariohi
    • , Marie Gendrel
    • , Florian Lesage
    • , Eun-Jin Kim
    • , Delphine Bichet
    • , Olga Andrini
    •  & Thomas Boulin
  • Article | | open

    Meiotic crossover (CO) landscape differs inter- and intra-species, as well as between sexes. Here, the authors show that male meiosis produces more COs than female in maize and detect CO maturation inefficiency in some genetic backgrounds, which may help to improve breeding efficiency.

    • Cheng Luo
    • , Xiang Li
    • , Qinghua Zhang
    •  & Jianbing Yan
  • Article | | open

    Molecular motion has attracted a wide range of interest for different applications. Here, the authors develop nanoparticles with internal molecular motion upon near infrared absorption and use the nanoparticles for photoacoustic imaging and demonstrate this application in vivo.

    • Zheng Zhao
    • , Chao Chen
    • , Wenting Wu
    • , Fenfen Wang
    • , Lili Du
    • , Xiaoyan Zhang
    • , Yu Xiong
    • , Xuewen He
    • , Yuanjing Cai
    • , Ryan T. K. Kwok
    • , Jacky W. Y. Lam
    • , Xike Gao
    • , Pingchuan Sun
    • , David Lee Phillips
    • , Dan Ding
    •  & Ben Zhong Tang
  • Article | | open

    Infection of a cell with influenza B virus (IBV) often results in cell death and the role of surviving cells in pathogenesis is unclear. Here, Dumm et al. generate a recombinant IBV that activates a host-cell reporter to permanently label infected cells, and show that surviving cells are important to preserve epithelial barrier function.

    • Rebekah E. Dumm
    • , Jessica K. Fiege
    • , Barbara M. Waring
    • , Chay T. Kuo
    • , Ryan A. Langlois
    •  & Nicholas S. Heaton
  • Article | | open

    Defect tolerance is important in order to ensure the mechanical integrity of synthetic materials and biological tissues. Here the authors demonstrate that toughening mechanisms provide soft collagenous tissues with high defect tolerance, with important implications for biomedical applications.

    • Kevin Bircher
    • , Manuel Zündel
    • , Marco Pensalfini
    • , Alexander E. Ehret
    •  & Edoardo Mazza
  • Article | | open

    Pseudomonas aeruginosa releases redox-active metabolites called phenazines. Here, the authors use metabolic imaging by stimulated Raman scattering microscopy to show that phenazines antagonize the effects of antibiotics on P. aeruginosa biofilms by modulating bacterial metabolism.

    • Konstanze T. Schiessl
    • , Fanghao Hu
    • , Jeanyoung Jo
    • , Sakila Z. Nazia
    • , Bryan Wang
    • , Alexa Price-Whelan
    • , Wei Min
    •  & Lars E. P. Dietrich
  • Article | | open

    Viruses can encode genes that regulate the host's translational machinery to their advantage. Here, the authors show that viruses encode ribosomal proteins that can be incorporated into the host’s ribosome and may affect translation.

    • Carolina M. Mizuno
    • , Charlotte Guyomar
    • , Simon Roux
    • , Régis Lavigne
    • , Francisco Rodriguez-Valera
    • , Matthew B. Sullivan
    • , Reynald Gillet
    • , Patrick Forterre
    •  & Mart Krupovic
  • Article | | open

    Coccolithophores are one of the most abundant phytoplankton and calcifying organisms, well-known to produce intricate calcareous exoskeletons made of coccoliths. Here the authors show, by using X-ray nanotomography, the dependence of the grid size on the calcite nucleation site number and on the mass of coccoliths.

    • T. Beuvier
    • , I. Probert
    • , L. Beaufort
    • , B. Suchéras-Marx
    • , Y. Chushkin
    • , F. Zontone
    •  & A. Gibaud
  • Article | | open

    Here, Depledge et al. use nanopore arrays for direct RNA sequencing to profile the HSV-1 transcriptome in productively infected cells. Sequencing of individual RNAs reveals a highly complex viral transcriptome including mRNAs encoding new viral fusion proteins derived by read-through transcription.

    • Daniel P. Depledge
    • , Kalanghad Puthankalam Srinivas
    • , Tomohiko Sadaoka
    • , Devin Bready
    • , Yasuko Mori
    • , Dimitris G. Placantonakis
    • , Ian Mohr
    •  & Angus C. Wilson
  • Article | | open

    Adenovirus based (AdV) vectors are promising platforms for therapeutics and vaccines, but receptor usage of serotypes in clinical development remains unclear. Here, based on crystal structures and modeling, Baker et al. show that HAdV-D26/48 fiber knob protein interacts weakly with CAR but not with CD46 or DSG2.

    • Alexander T. Baker
    • , Alexander Greenshields-Watson
    • , Lynda Coughlan
    • , James A. Davies
    • , Hanni Uusi-Kerttula
    • , David K. Cole
    • , Pierre J. Rizkallah
    •  & Alan L. Parker
  • Article | | open

    Few approaches for targeted manipulation of the epigenome are available in plants. Here, the authors adapt the dCas9-SunTag system to engineer targeted gene activation and site-specific manipulation of DNA methylation in Arabidopsis.

    • Ashot Papikian
    • , Wanlu Liu
    • , Javier Gallego-Bartolomé
    •  & Steven E. Jacobsen
  • Article | | open

    Quaternary contacts mediated by an extended heavy-chain framework region 3 (FR3) have been shown to improve binding to HIV envelope and virus neutralization for a few antibodies. Here, Liu et al. engraft such an FR3 loop onto several potent broadly neutralizing antibodies, resulting in improved neutralization activity and pharmacokinetics.

    • Qingbo Liu
    • , Yen-Ting Lai
    • , Peng Zhang
    • , Mark K. Louder
    • , Amarendra Pegu
    • , Reda Rawi
    • , Mangaiarkarasi Asokan
    • , Xuejun Chen
    • , Chen-Hsiang Shen
    • , Gwo-Yu Chuang
    • , Eun Sung Yang
    • , Huiyi Miao
    • , Yuge Wang
    • , Anthony S. Fauci
    • , Peter D. Kwong
    • , John R. Mascola
    •  & Paolo Lusso
  • Article | | open

    While methane has become an increasingly important chemical feedstock, its selective conversion to other chemicals has proven challenging. Here, authors demonstrate a photocatalyst based on earth-abundant elements that selectively converts methane to carbon monoxide under mild conditions.

    • Xiang Yu
    • , Vincent De Waele
    • , Axel Löfberg
    • , Vitaly Ordomsky
    •  & Andrei Y. Khodakov
  • Article | | open

    The search for peroxidase-like as well as other enzyme-like nanozymes mainly relies on trial-and-error strategies, due to the lack of predictive descriptors. Here, the authors fill this gap by investigating the occupancy of eg orbitals as a possible descriptor for the peroxidase-like activity of transition metal oxide nanozymes

    • Xiaoyu Wang
    • , Xuejiao J. Gao
    • , Li Qin
    • , Changda Wang
    • , Li Song
    • , Yong-Ning Zhou
    • , Guoyin Zhu
    • , Wen Cao
    • , Shichao Lin
    • , Liqi Zhou
    • , Kang Wang
    • , Huigang Zhang
    • , Zhong Jin
    • , Peng Wang
    • , Xingfa Gao
    •  & Hui Wei
  • Article | | open

    While the conversion of CO2 to valuable, storable chemicals is attractive, there are few inexpensive and abundant catalysts that are also active and selective for liquid fuels. Here, the authors study copper selenide as a high-performing and efficient electrocatalyst for CO2 conversion to methanol.

    • Dexin Yang
    • , Qinggong Zhu
    • , Chunjun Chen
    • , Huizhen Liu
    • , Zhimin Liu
    • , Zhijuan Zhao
    • , Xiaoyu Zhang
    • , Shoujie Liu
    •  & Buxing Han
  • Article | | open

    We know a large number of risk SNPs for schizophrenia, but little about how these SNPs contribute to the disorder. Here, the authors use functional genomics to identify risk SNPs that disrupt transcription factor binding and validate the regulatory effects of the transcription factor binding-disrupting SNPs.

    • Yongxia Huo
    • , Shiwu Li
    • , Jiewei Liu
    • , Xiaoyan Li
    •  & Xiong-Jian Luo
  • Article | | open

    Many Gram-negative bacteria rely on a type III secretion system (T3SS) for their pathogenicity. Here authors present the cryo-EM structure of the E.coli T3SS ATPase-central stalk complex, which forms a homohexameric, asymmetric pore with different functional states.

    • Dorothy D. Majewski
    • , Liam J. Worrall
    • , Chuan Hong
    • , Claire E. Atkinson
    • , Marija Vuckovic
    • , Nobuhiko Watanabe
    • , Zhiheng Yu
    •  & Natalie C. J. Strynadka
  • Article | | open

    Transposable element (TE) bursts shape genome evolution but their origin remains unclear. Here, the authors show that a burst is restricted to only a few domesticated rice accessions and is associated with the acquisition of two TE variants, Ping16A and Ping16A_Stow, not the loss of TE silencing.

    • Jinfeng Chen
    • , Lu Lu
    • , Jazmine Benjamin
    • , Stephanie Diaz
    • , C. Nathan Hancock
    • , Jason E. Stajich
    •  & Susan R. Wessler
  • Article | | open

    Development of bacterial resistance to an antibiotic can lead to collateral sensitivity to another drug. Here, the authors study collateral sensitivity conferred by mutations in the horizontally acquired β-lactamase CTX-M-15, and identify antibiotic combinations that constrain the evolution of resistance.

    • Carola E. H. Rosenkilde
    • , Christian Munck
    • , Andreas Porse
    • , Marius Linkevicius
    • , Dan I. Andersson
    •  & Morten O. A. Sommer
  • Article | | open

    Hematopoietic stem cell (HSC) transplantation is a desirable treatment for many non-malignant and malignant diseases, but its use requires preconditioning of recipients with irradiation or chemotherapy that often induces high toxicity. Here the authors show that antibody-drug-conjugate to CD117, a HSC marker, allows specific and efficient preconditioning for HSC therapy.

    • Agnieszka Czechowicz
    • , Rahul Palchaudhuri
    • , Amelia Scheck
    • , Yu Hu
    • , Jonathan Hoggatt
    • , Borja Saez
    • , Wendy W. Pang
    • , Michael K. Mansour
    • , Tiffany A. Tate
    • , Yan Yi Chan
    • , Emily Walck
    • , Gerlinde Wernig
    • , Judith A. Shizuru
    • , Florian Winau
    • , David T. Scadden
    •  & Derrick J. Rossi
  • Article | | open

    The use of pluripotent-stem cell derived organs for transplantation would be promising, if organs can be grown in a suitable host. Here, the authors use interspecific blastocyst complementation to generate a mouse pluripotent stem cell-derived kidney in anephric Sall1 mutant rats.

    • Teppei Goto
    • , Hiromasa Hara
    • , Makoto Sanbo
    • , Hideki Masaki
    • , Hideyuki Sato
    • , Tomoyuki Yamaguchi
    • , Shinichi Hochi
    • , Toshihiro Kobayashi
    • , Hiromitsu Nakauchi
    •  & Masumi Hirabayashi
  • Article | | open

    Antibodies against Plasmodium falciparum merozoites that fix complement can inhibit blood-stage replication. Here, Reiling et al. show that complement-fixing antibodies strongly correlate with protective immunity in children, identify the merozoite targets, and predict antigen combinations that should result in strong protection.

    • Linda Reiling
    • , Michelle J. Boyle
    • , Michael T. White
    • , Danny W. Wilson
    • , Gaoqian Feng
    • , Rupert Weaver
    • , D. Herbert Opi
    • , Kristina E. M. Persson
    • , Jack S. Richards
    • , Peter M. Siba
    • , Freya J. I. Fowkes
    • , Eizo Takashima
    • , Takafumi Tsuboi
    • , Ivo Mueller
    •  & James G. Beeson
  • Article | | open

    End-product toxicity, culture contamination, and energy efficient product recovery are long-standing issues in bioprocessing. Here, the authors address these problems using a fermentation strategy that combines microbial production of branched alcohols with supercritical carbon dioxide extraction.

    • Jason T. Boock
    • , Adam J. E. Freedman
    • , Geoffrey A. Tompsett
    • , Sarah K. Muse
    • , Audrey J. Allen
    • , Luke A. Jackson
    • , Bernardo Castro-Dominguez
    • , Michael T. Timko
    • , Kristala L. J. Prather
    •  & Janelle R. Thompson
  • Article | | open

    Heart grafts need good vascularization to survive. Here, the authors engineer perfusable constructs of human embryonic stem cell-derived endothelial cells seeded in collagen matrix in patterned microchannels that form anastomosed vessels in vitro and have increased coronary vascular perfusion on transplantation in rats.

    • Meredith A. Redd
    • , Nicole Zeinstra
    • , Wan Qin
    • , Wei Wei
    • , Amy Martinson
    • , Yuliang Wang
    • , Ruikang K. Wang
    • , Charles E. Murry
    •  & Ying Zheng
  • Article | | open

    N6-methyladenine is involved in many biological pathways for microbial survival and host interaction. Here the authors train a neural network for improved m6A detection in nanopore sequencing data and validate methylomes for a microbial reference community.

    • Alexa B. R. McIntyre
    • , Noah Alexander
    • , Kirill Grigorev
    • , Daniela Bezdan
    • , Heike Sichtig
    • , Charles Y. Chiu
    •  & Christopher E. Mason
  • Article | | open

    The genomic locations that can be targeted for editing by CRISPR are limited by the presence of the nuclease-specific PAM sequence. Here, the authors show PAM recognition can be expanded by replacing the key region in the PAM interaction domain of SaCas9 with the corresponding region of SaCas9 orthologs.

    • Dacheng Ma
    • , Zhimeng Xu
    • , Zhaoyu Zhang
    • , Xi Chen
    • , Xiangzhi Zeng
    • , Yiyang Zhang
    • , Tingyue Deng
    • , Mengfei Ren
    • , Zheng Sun
    • , Rui Jiang
    •  & Zhen Xie
  • Article | | open

    Self-assembly of carbohydrates play an integral part in the design of higher ordered structures, but is limited to amphiphiles where the carbohydrate is covalently bound to a hydrophobic tail. Here the authors show that sugars direct the self-assembly of insoluble curcumin and the formation of well-defined capsules.

    • Sandy Wong
    • , Jiacheng Zhao
    • , Cheng Cao
    • , Chin Ken Wong
    • , Rhiannon P. Kuchel
    • , Sergio De Luca
    • , James M. Hook
    • , Christopher J. Garvey
    • , Sean Smith
    • , Junming Ho
    •  & Martina H. Stenzel
  • Article | | open

    The Caucasus mountain range has impacted on the culture and genetics of the wider region. Here, the authors generate genome-wide SNP data for 45 Eneolithic and Bronze Age individuals across the Caucasus, and find distinct genetic clusters between mountain and steppe zones as well as occasional gene-flow.

    • Chuan-Chao Wang
    • , Sabine Reinhold
    • , Alexey Kalmykov
    • , Antje Wissgott
    • , Guido Brandt
    • , Choongwon Jeong
    • , Olivia Cheronet
    • , Matthew Ferry
    • , Eadaoin Harney
    • , Denise Keating
    • , Swapan Mallick
    • , Nadin Rohland
    • , Kristin Stewardson
    • , Anatoly R. Kantorovich
    • , Vladimir E. Maslov
    • , Vladimira G. Petrenko
    • , Vladimir R. Erlikh
    • , Biaslan Ch. Atabiev
    • , Rabadan G. Magomedov
    • , Philipp L. Kohl
    • , Kurt W. Alt
    • , Sandra L. Pichler
    • , Claudia Gerling
    • , Harald Meller
    • , Benik Vardanyan
    • , Larisa Yeganyan
    • , Alexey D. Rezepkin
    • , Dirk Mariaschk
    • , Natalia Berezina
    • , Julia Gresky
    • , Katharina Fuchs
    • , Corina Knipper
    • , Stephan Schiffels
    • , Elena Balanovska
    • , Oleg Balanovsky
    • , Iain Mathieson
    • , Thomas Higham
    • , Yakov B. Berezin
    • , Alexandra Buzhilova
    • , Viktor Trifonov
    • , Ron Pinhasi
    • , Andrej B. Belinskij
    • , David Reich
    • , Svend Hansen
    • , Johannes Krause
    •  & Wolfgang Haak
  • Article | | open

    Producing individual cannabinoids by metabolically engineered microbes has proven challenging. Here, the authors develop a cell-free enzymatic prenylating system to generate isoprenyl pyrophosphate substrates directly from glucose and produce both common and rare cannabinoids at >1 g/L.

    • Meaghan A. Valliere
    • , Tyler P. Korman
    • , Nicholas B. Woodall
    • , Gregory A. Khitrov
    • , Robert E. Taylor
    • , David Baker
    •  & James U. Bowie
  • Article | | open

    Previous surveys of global ocean microbial diversity have focused on planktonic microbes. Here, Zhang et al. use metagenomics to study biofilm-forming marine microbes, increasing the known microbial diversity in the oceans by more than 20% and revealing new biosynthetic gene clusters and CRISPR-Cas systems.

    • Weipeng Zhang
    • , Wei Ding
    • , Yong-Xin Li
    • , Chunkit Tam
    • , Salim Bougouffa
    • , Ruojun Wang
    • , Bite Pei
    • , Hoyin Chiang
    • , Pokman Leung
    • , Yanhong Lu
    • , Jin Sun
    • , He Fu
    • , Vladimir B Bajic
    • , Hongbin Liu
    • , Nicole S. Webster
    •  & Pei-Yuan Qian
  • Article | | open

    Host microbiomes are feasible sources for drug discovery. Here, using large-scale isolations, bioactivity assays and omics, the authors uncover the antimicrobial potential of insect-associated Streptomyces and identify a compound, cyphomycin, active against multidrug-resistant fungal pathogens.

    • Marc G. Chevrette
    • , Caitlin M. Carlson
    • , Humberto E. Ortega
    • , Chris Thomas
    • , Gene E. Ananiev
    • , Kenneth J. Barns
    • , Adam J. Book
    • , Julian Cagnazzo
    • , Camila Carlos
    • , Will Flanigan
    • , Kirk J. Grubbs
    • , Heidi A. Horn
    • , F. Michael Hoffmann
    • , Jonathan L. Klassen
    • , Jennifer J. Knack
    • , Gina R. Lewin
    • , Bradon R. McDonald
    • , Laura Muller
    • , Weilan G. P. Melo
    • , Adrián A. Pinto-Tomás
    • , Amber Schmitz
    • , Evelyn Wendt-Pienkowski
    • , Scott Wildman
    • , Miao Zhao
    • , Fan Zhang
    • , Tim S. Bugni
    • , David R. Andes
    • , Monica T. Pupo
    •  & Cameron R. Currie
  • Article | | open

    Combination therapy for Buruli ulcer (BU) is suboptimal. Here, Liu et al. show that the candidate drug TB47 has potent bactericidal activity against Mycobacterium ulcerans in vitro and in a mouse model, which underscores its potential for shortening the course of BU and treating other mycobacterial diseases.

    • Yang Liu
    • , Yamin Gao
    • , Jianxiong Liu
    • , Yaoju Tan
    • , Zhiyong Liu
    • , Chiranjibi Chhotaray
    • , Huofeng Jiang
    • , Zhili Lu
    • , Gift Chiwala
    • , Shuai Wang
    • , Gaelle Makafe
    • , Md Mahmudul Islam
    • , H. M. Adnan Hameed
    • , Xingshan Cai
    • , Changwei Wang
    • , Xinjie Li
    • , Shouyong Tan
    •  & Tianyu Zhang
  • Article | | open

    Lineage segregation from conception to gastrulation has been mapped at the single cell level in mouse, human and monkey. Here, the authors provide a comprehensive analysis of porcine preimplantation development using single cell RNA-seq; mapping metabolic changes, X chromosome inactivation and signalling pathways.

    • Priscila Ramos-Ibeas
    • , Fei Sang
    • , Qifan Zhu
    • , Walfred W. C. Tang
    • , Sarah Withey
    • , Doris Klisch
    • , Liam Wood
    • , Matt Loose
    • , M. Azim Surani
    •  & Ramiro Alberio
  • Article | | open

    Adeno-associated viral vectors (AAV) are being developed for gene therapy of skeletal muscle, but it is a challenge to achieve robust gene expression. Here, the authors identify muscle-specific cisregulatory elements that lead to a substantial increase in micro-dystrophin and follistatin expression, resulting in a safe and sustainable rescue of the dystrophic phenotype in mouse models.

    • S. Sarcar
    • , W. Tulalamba
    • , M. Y. Rincon
    • , J. Tipanee
    • , H. Q. Pham
    • , H. Evens
    • , D. Boon
    • , E. Samara-Kuko
    • , M. Keyaerts
    • , M. Loperfido
    • , E. Berardi
    • , S. Jarmin
    • , P. In’t Veld
    • , G. Dickson
    • , T. Lahoutte
    • , M. Sampaolesi
    • , P. De Bleser
    • , T. VandenDriessche
    •  & M. K. Chuah
  • Article | | open

    Lead trihalide perovskites are notable for their excellent optoelectronic properties and uncommon phase behavior. Here, Kirschner et al. show that cesium lead bromide nanocrystals experience a reversible orthorhombic-to-cubic phase transition at moderate excitation fluences and become amorphous at higher fluences.

    • Matthew S. Kirschner
    • , Benjamin T. Diroll
    • , Peijun Guo
    • , Samantha M. Harvey
    • , Waleed Helweh
    • , Nathan C. Flanders
    • , Alexandra Brumberg
    • , Nicolas E. Watkins
    • , Ariel A. Leonard
    • , Austin M. Evans
    • , Michael R. Wasielewski
    • , William R. Dichtel
    • , Xiaoyi Zhang
    • , Lin X. Chen
    •  & Richard D. Schaller
  • Article | | open

    Host-parasite interactions during the exoerythrocytic stage of Plasmodium infection remains poorly understood. Using dual RNA-Seq, the authors show that human mucosal immunity protein mucin-13 is upregulated during Plasmodium hepatic-stage infection and marks infected cells independent of tested Plasmodium species.

    • Gregory M. LaMonte
    • , Pamela Orjuela-Sanchez
    • , Jaeson Calla
    • , Lawrence T. Wang
    • , Shangzhong Li
    • , Justine Swann
    • , Annie N. Cowell
    • , Bing Yu Zou
    • , Alyaa M. Abdel-Haleem Mohamed
    • , Zaira Hellen Villa Galarce
    • , Marta Moreno
    • , Carlos Tong Rios
    • , Joseph M. Vinetz
    • , Nathan Lewis
    •  & Elizabeth A. Winzeler
  • Article | | open

    Heterogeneity in gene expression and epigenetic states exists across individual cells. Here, the authors develop scCAT-seq, a technique for simultaneously performing ATAC-seq and RNA-seq within the same single cell.

    • Longqi Liu
    • , Chuanyu Liu
    • , Andrés Quintero
    • , Liang Wu
    • , Yue Yuan
    • , Mingyue Wang
    • , Mengnan Cheng
    • , Lizhi Leng
    • , Liqin Xu
    • , Guoyi Dong
    • , Rui Li
    • , Yang Liu
    • , Xiaoyu Wei
    • , Jiangshan Xu
    • , Xiaowei Chen
    • , Haorong Lu
    • , Dongsheng Chen
    • , Quanlei Wang
    • , Qing Zhou
    • , Xinxin Lin
    • , Guibo Li
    • , Shiping Liu
    • , Qi Wang
    • , Hongru Wang
    • , J. Lynn Fink
    • , Zhengliang Gao
    • , Xin Liu
    • , Yong Hou
    • , Shida Zhu
    • , Huanming Yang
    • , Yunming Ye
    • , Ge Lin
    • , Fang Chen
    • , Carl Herrmann
    • , Roland Eils
    • , Zhouchun Shang
    •  & Xun Xu
  • Article | | open

    Most cyanobacteria are oxygenic photoautotrophs, and fermenters under dark anoxic conditions. Here, the authors analyse genomic sequences of related uncultivated bacteria, inferring their metabolic potential, and supporting that their common ancestor was an anaerobe capable of fermentation and H2 metabolism.

    • Paula B. Matheus Carnevali
    • , Frederik Schulz
    • , Cindy J. Castelle
    • , Rose S. Kantor
    • , Patrick M. Shih
    • , Itai Sharon
    • , Joanne M. Santini
    • , Matthew R. Olm
    • , Yuki Amano
    • , Brian C. Thomas
    • , Karthik Anantharaman
    • , David Burstein
    • , Eric D. Becraft
    • , Ramunas Stepanauskas
    • , Tanja Woyke
    •  & Jillian F. Banfield
  • Article | | open

    Genetic logic devices allow the host cell to incorporate multiple inputs to determine output behaviour. Here the authors provide a framework for engineering reliable recombinase-based devices and demonstrate 4-input logic in a multicellular system.

    • Sarah Guiziou
    • , Pauline Mayonove
    •  & Jerome Bonnet
  • Article | | open

    Broomcorn millet is one of the earliest domesticated plants and has the highest water use efficiency among cereals. Here, the authors report its genome assembly and annotation, which provides a valuable resource for breeders and paves the way for studying plant drought tolerance and C4 photosynthesis.

    • Changsong Zou
    • , Leiting Li
    • , Daisuke Miki
    • , Delin Li
    • , Qiming Tang
    • , Lihong Xiao
    • , Santosh Rajput
    • , Ping Deng
    • , Li Peng
    • , Wei Jia
    • , Ru Huang
    • , Meiling Zhang
    • , Yidan Sun
    • , Jiamin Hu
    • , Xing Fu
    • , Patrick S. Schnable
    • , Yuxiao Chang
    • , Feng Li
    • , Hui Zhang
    • , Baili Feng
    • , Xinguang Zhu
    • , Renyi Liu
    • , James C. Schnable
    • , Jian-Kang Zhu
    •  & Heng Zhang
  • Article | | open

    Multiple morphological abnormalities of the sperm flagella (MMAF) is a cause of male infertility. Here the authors identify homozygous nonsense mutations of the glutamine rich 2 (QRICH2) gene in two MMAF patients from 2 consanguineous families and show using QRICH2 knockout mice that the protein is required for sperm flagellar formation and motility.

    • Ying Shen
    • , Feng Zhang
    • , Fuping Li
    • , Xiaohui Jiang
    • , Yihong Yang
    • , Xiaoliang Li
    • , Weiyu Li
    • , Xiang Wang
    • , Juan Cheng
    • , Mohan Liu
    • , Xueguang Zhang
    • , Guiping Yuan
    • , Xue Pei
    • , Kailai Cai
    • , Fengyun Hu
    • , Jianfeng Sun
    • , Lanzhen Yan
    • , Li Tang
    • , Chuan Jiang
    • , Wenling Tu
    • , Jinyan Xu
    • , Haojuan Wu
    • , Weiqi Kong
    • , Shuying Li
    • , Ke Wang
    • , Kai Sheng
    • , Xudong Zhao
    • , Huanxun Yue
    • , Xiaoyu Yang
    •  & Wenming Xu
  • Article | | open

    The delivery of single therapeutic microRNAs in brain cancers is challenging. Here, the authors engineer three neuronal microRNAs (miR-124, 128 and 137) into a cluster that, targeting oncogenic chromatin repressors, increases survival of GBM-bearing mice when combined with chemotherapy.

    • Vivek Bhaskaran
    • , Michal O. Nowicki
    • , Mahmoud Idriss
    • , Miguel A. Jimenez
    • , Gianmarco Lugli
    • , Josie L. Hayes
    • , Ahmad Bakur Mahmoud
    • , Rachel E. Zane
    • , Carmela Passaro
    • , Keith L. Ligon
    • , Daphne Haas-Kogan
    • , Agnieszka Bronisz
    • , Jakub Godlewski
    • , Sean E. Lawler
    • , E. Antonio Chiocca
    •  & Pierpaolo Peruzzi
  • Article | | open

    Broomcorn millet is one of the oldest crops cultivated by human that has strong abiotic stress tolerance. To facilitate genome assisted breeding of this and related species, the authors report its genome assembly and conduct comparative genome structure and evolution analyses with foxtail millet.

    • Junpeng Shi
    • , Xuxu Ma
    • , Jihong Zhang
    • , Yingsi Zhou
    • , Minxuan Liu
    • , Liangliang Huang
    • , Silong Sun
    • , Xiangbo Zhang
    • , Xiang Gao
    • , Wei Zhan
    • , Pinghua Li
    • , Lun Wang
    • , Ping Lu
    • , Haiming Zhao
    • , Weibin Song
    •  & Jinsheng Lai