Article
|
Open Access
Featured
-
-
Article
| Open AccessInsertion-sequence-mediated mutations both promote and constrain evolvability during a long-term experiment with bacteria
Insertion sequences (IS) are common mobile genetic elements in bacteria, but their effects on bacterial evolution are not well understood. Here, Consuegra and colleagues investigate the dynamics and fitness consequences of IS elements in E. coli over 50,000 generations.
- Jessika Consuegra
- , Joël Gaffé
- & Dominique Schneider
-
Article
| Open AccessPRMT5 inhibition disrupts splicing and stemness in glioblastoma
The arginine methyltransferase PRMT5 is over-expressed in cancer and has a role in the maintenance of stem cells. Here, the authors show that PRMT5 inhibitors can block the growth of patient derived glioblastoma stem cell cultures in vitro and in vivo, suggesting that PRMT5 inhibition may be a useful therapeutic strategy
- Patty Sachamitr
- , Jolene C. Ho
- & Peter B. Dirks
-
Article
| Open AccessC. elegans germ granules require both assembly and localized regulators for mRNA repression
Nematode P granules are cytoplasmic RNA–protein biomolecule condensates central to germ cell development. Here the authors show that dimerization of the PGL-1 scaffolding protein is crucial to granule formation and mRNA repression, and that the WAGO-1 Argonaute protein is a cofactor in repressing PGL-1 bound mRNAs.
- Scott Takeo Aoki
- , Tina R. Lynch
- & Judith Kimble
-
Matters Arising
| Open AccessSulfisoxazole does not inhibit the secretion of small extracellular vesicles
- Pamali Fonseka
- , Sai V. Chitti
- & Suresh Mathivanan
-
Article
| Open AccessPersistence of self-reactive CD8+ T cells in the CNS requires TOX-dependent chromatin remodeling
The transcriptional adaptation processes of harmful self-reactive CD8+ T cells in the central nervous system are not well understood. Here the authors use a system in which self-reactive and virally generated CD8+ T cells are directly compared in vivo and demonstrate that TOX expression contributes to maintenance of auto-reactive CD8+ T cells through alteration of chromatin accessibility.
- Nicolas Page
- , Sylvain Lemeille
- & Doron Merkler
-
Article
| Open AccessCross-cancer evaluation of polygenic risk scores for 16 cancer types in two large cohorts
While genetic loci shared between cancer types have been identified, cross-cancer relationships for polygenic risk scores have not been well studied. Here, the authors have developed polygenic risk scores for 16 cancers in two large cohorts and identified positive and inverse cross-cancer associations.
- Rebecca E. Graff
- , Taylor B. Cavazos
- & Lori C. Sakoda
-
Article
| Open AccessEfficacy in deceptive vocal exaggeration of human body size
While size exaggeration is common in the animal kingdom, Pisanski & Reby show that human listeners can detect deceptive vocal signals of people trying to sound bigger or smaller, and recalibrate their estimates accordingly, especially men judging the heights of other men, with implications for the evolution of vocal communication.
- Katarzyna Pisanski
- & David Reby
-
Article
| Open AccessEGCG binds intrinsically disordered N-terminal domain of p53 and disrupts p53-MDM2 interaction
Epigallocatechin gallate (EGCG) is a catechin flavonoid which induces apoptosis in cancerous cells, but the underlying molecular mechanisms remain poorly understood. Here authors use an interdisciplinary approach to show a direct interaction between EGCG and the tumor suppressor p53 and demonstrate that EGCG inhibits ubiquitination of p53 by MDM2.
- Jing Zhao
- , Alan Blayney
- & Chunyu Wang
-
Article
| Open AccessMagneto-optical diagnosis of symptomatic malaria in Papua New Guinea
Here Arndt et al. establish rotating-crystal magneto-optical detection (RMOD) as a near-point-of-care diagnostic tool for malaria detection and report a sensitivity and specificity of 82% and 84%, respectively, as validated by analyzing a clinical population in a high transmission setting in Papua New Guinea.
- L. Arndt
- , T. Koleala
- & S. Karl
-
Article
| Open AccessGenomic aberrations after short-term exposure to colibactin-producing E. coli transform primary colon epithelial cells
Colibactin-producing pks+ Escherichia coli are frequent constituents of the human intestinal microbiota. Here, the authors show that short exposure of cells to pks+ E. coli induces chromosomal aberrations, genomic instability, and multiple features of transformation reminiscent of colorectal cancer.
- Amina Iftekhar
- , Hilmar Berger
- & Thomas F. Meyer
-
Article
| Open AccessGranulovirus PK-1 kinase activity relies on a side-to-side dimerization mode centered on the regulatory αC helix
The viral Protein Kinase-1 (PK-1) phosphorylates the regulatory protein p6.9, which facilitates baculoviral genome release. Here, the authors combine X-ray crystallography with biophysical and biochemical analyses as well as molecular dynamics simulations to characterize Cydia pomenella granulovirus PK-1, which forms a dimer with a parallel side-to-side arrangement of the kinase domains and furthermore, they provide insights into its catalytic mechanism and evolutionary relationships with other kinases.
- Michael R. Oliver
- , Christopher R. Horne
- & James M. Murphy
-
Article
| Open AccessHow μ-opioid receptor recognizes fentanyl
Structures of mu-opioid receptor (mOR) in complex with morphine derivatives have been determined; but the structural basis of mOR activation by fentanyl-like synthetic opioids remains unclear. Here, authors use state-of-the-art simulation techniques and discover a secondary binding mode which is only accessible when the conserved His297 adopts a neutral HID tautomer state.
- Quynh N. Vo
- , Paween Mahinthichaichan
- & Christopher R. Ellis
-
Article
| Open AccessMultiple mechanisms drive genomic adaptation to extreme O2 levels in Drosophila melanogaster
The genomic details of adaptation to extreme environments remain challenging to characterize. Using new methods to analyze flies experimentally evolved to survive extreme O2 conditions, the authors find a surprising level of synchronicity in selective sweeps, de novo mutations and adaptive recombination events.
- Arya Iranmehr
- , Tsering Stobdan
- & Gabriel G. Haddad
-
Article
| Open AccessTumor cell plasticity, heterogeneity, and resistance in crucial microenvironmental niches in glioma
Whether the perivascular niche (PVN) and the integration into multicellular networks by tumor microtubes (TMs) have a different role in glioblastoma progression and resistance to therapies is currently unclear. Here, the authors, by long-term tracking of individual glioma, demonstrate that both niches can partially compensate for each other and that glioma cells localized in both niches are resistant to radio- and chemotherapy.
- Erik Jung
- , Matthias Osswald
- & Frank Winkler
-
Article
| Open AccessAA amyloid fibrils from diseased tissue are structurally different from in vitro formed SAA fibrils
Systemic AA amyloidosis is a protein misfolding disease caused by the formation of amyloid fibrils from serum amyloid A (SAA) protein. Here, the authors present the cryo-EM structures of AA amyloid fibrils isolated from mouse tissue and in vitro formed fibrils, which differ in their structures and they also show that the ex vivo fibrils are more resistant to proteolysis than the in vitro fibrils and propose that pathogenic amyloid fibrils might originate from proteolytic selection.
- Akanksha Bansal
- , Matthias Schmidt
- & Marcus Fändrich
-
Article
| Open AccessDual targeting of polyamine synthesis and uptake in diffuse intrinsic pontine gliomas
Diffuse intrinsic pontine glioma (DIPG) is an almost incurable malignant childhood brain tumor. Here, the authors show that the polyamine synthetic pathway is activated in DIPG and that the dual targeting of polyamine synthesis and uptake results in prolonged survival in animal models.
- Aaminah Khan
- , Laura D. Gamble
- & David S. Ziegler
-
Article
| Open AccessImpact of the COVID-19 nonpharmaceutical interventions on influenza and other respiratory viral infections in New Zealand
New Zealand has been relatively successful in controlling COVID-19 due to implementation of strict non-pharmaceutical interventions. Here, the authors demonstrate a striking decline in reports of influenza and other non-influenza respiratory pathogens over winter months in which the interventions have been in place.
- Q. Sue Huang
- , Tim Wood
- & Richard J. Webby
-
Review Article
| Open AccessRenal metabolism and hypertension
Hypertension is one of the most important risk factors for cardiovascular disease. The kidneys, which have a very high metabolic rate, play a fundamental role in blood pressure regulation. In this review, the authors discuss recent studies on the role of renal metabolism in the development of hypertension.
- Zhongmin Tian
- & Mingyu Liang
-
Article
| Open AccessSelf-assembly and regulation of protein cages from pre-organised coiled-coil modules
Coiled-coil protein origami is a strategy for the de novo design of polypeptide nanostructures based on coiled-coil dimer forming peptides, where a single chain protein folds into a polyhedral cage. Here, the authors design a single-chain triangular bipyramid and also demonstrate that the bipyramid can be self-assembled as a heterodimeric complex, comprising pre-defined subunits.
- Fabio Lapenta
- , Jana Aupič
- & Roman Jerala
-
Article
| Open AccessRNA secondary structure prediction using deep learning with thermodynamic integration
Accurately predicting the secondary structure of non-coding RNAs can help unravel their function. Here the authors propose a method integrating thermodynamic information and deep learning to improve the robustness of RNA secondary structure prediction compared to several existing algorithms.
- Kengo Sato
- , Manato Akiyama
- & Yasubumi Sakakibara
-
Article
| Open AccessRegulatory inter-domain interactions influence Hsp70 recruitment to the DnaJB8 chaperone
The Hsp70/Hsp40 system plays an important role in maintaining cellular proteostasis but so far it is not well understood how Hsp70 proteins are recruited to specific Hsp40 co-chaperones. Here, the authors combine biochemical and biophysical approaches to characterise the oligomeric mammalian Hsp40 DnaJB8. They identify an intra-oligomer DnaJB8 interaction between the N-terminal J-Domain and the C-terminal domain that occludes the J-Domain surface that binds Hsp70 and propose a model for DnaJB8-Hsp70 recruitment.
- Bryan D. Ryder
- , Irina Matlahov
- & Lukasz A. Joachimiak
-
Article
| Open AccessDesigned folding pathway of modular coiled-coil-based proteins
Coiled-coil protein origami (CCPO) is a strategy for the design of polyhedral cage-shaped protein folds based on coiled-coil (CC) dimer-forming peptides. Here, the authors show that CCPO proteins fold in a multistep process governed by the spatial distance between CC modules in the primary sequence and subsequent folding intermediates, which enables the use of identical CC modules for the CCPO tetrahedron design.
- Jana Aupič
- , Žiga Strmšek
- & Roman Jerala
-
Article
| Open AccessProduction of germ-free mosquitoes via transient colonisation allows stage-specific investigation of host–microbiota interactions
Germ-free mosquitoes generated with current methods exhibit developmental deficits. Here, the authors use genetically modified bacteria to allow complete decolonisation at any developmental stage of Aedes aegypti mosquitoes and show that bacteria support larval development by contributing to folate biosynthesis and enhancing energy storage.
- Ottavia Romoli
- , Johan Claes Schönbeck
- & Mathilde Gendrin
-
Article
| Open AccessA membrane-bound ankyrin repeat protein confers race-specific leaf rust disease resistance in wheat
Winter wheat cultivar Forno harbors a race-specific leaf rust resistance locus Lr14a, but the causative gene is unknown. Here, the authors show that Lr14a encodes a membrane-localized protein containing ankyrin repeats and Lr14a-containing segments have been introgressed into the bread wheat gene pool multiple times.
- Markus C. Kolodziej
- , Jyoti Singla
- & Beat Keller
-
Article
| Open AccessMechanisms of feedback inhibition and sequential firing of active sites in plant aspartate transcarbamoylase
Aspartate transcarbamoylase acts in de novo pyrimidine biosynthesis and in plants is regulated by feedback inhibition via uridine 5-monophosphate (UMP). Here Bellin et al. describe the structural basis for this feedback inhibition, showing that UMP blocks the active site by binding to a plant specific UMP recognition loop.
- Leo Bellin
- , Francisco Del Caño-Ochoa
- & Santiago Ramón-Maiques
-
Article
| Open AccessArabidopsis ACINUS is O-glycosylated and regulates transcription and alternative splicing of regulators of reproductive transitions
AtACINUS is an Arabidopsis homolog of a mammalian splicing regulator and previously found to be O-GlcNAcyated. Here Bi et al. characterize the interactors and targets of AtACINUS, show it is required for development and stress responses and provide evidence that its O-glycosylation affects alternative splicing.
- Yang Bi
- , Zhiping Deng
- & Zhi-Yong Wang
-
Article
| Open AccessStructural mechanism of bivalent histone H3K4me3K9me3 recognition by the Spindlin1/C11orf84 complex in rRNA transcription activation
Spindlin1 is an epigenetic reader that facilitates ribosomal RNA transcription. Here the authors reveal in vitro and structural evidence suggesting that Spindlin1 acts together with C11orf84 to recognize noncanonical bivalent mark of trimethylated lysine 4 and lysine 9 present on histone H3 tail (H3K4me3K9me3).
- Yongming Du
- , Yinxia Yan
- & Chengmin Qian
-
Article
| Open AccessTargeting IL-21 to tumor-reactive T cells enhances memory T cell responses and anti-PD-1 antibody therapy
The lack of an efficient anti-tumor T cell response contributes to the failure of anti-PD1 therapy. Here, the authors show a potential strategy to improve the therapeutic effects of anti-PD-1 antibody by simultaneously targeting IL-21 to tumor-reactive T cells in vivo.
- Ying Li
- , Yanni Cong
- & Shengdian Wang
-
Article
| Open AccessRare genetic variants affecting urine metabolite levels link population variation to inborn errors of metabolism
Metabolites are indicators of health and disease; genetic studies can reveal variants influencing their levels. Here, the authors investigate the contribution of rare, exonic variants on the levels of urine metabolites and generate predictions on metabolic consequences underlying metabolic disease.
- Yurong Cheng
- , Pascal Schlosser
- & Anna Köttgen
-
Article
| Open AccessPost-exposure protection of SARS-CoV-2 lethal infected K18-hACE2 transgenic mice by neutralizing human monoclonal antibody
Here, using the K18-hACE2 transgenic mice model, the authors report the in vivo efficacy of a fully human neutralizing antibody against SARS-CoV-2 and show that when administered before or up to 3 days post infection, treated mice do not exhibit disease symptoms while 80% of control animals succumb to the infection.
- Ronit Rosenfeld
- , Tal Noy-Porat
- & Ohad Mazor
-
Article
| Open AccessA genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry
The SARS-CoV-2 spike protein contains a multi-basic cleavage site. Here, the authors show how this multi-basic cleavage site affects entry of SARS-CoV-2 into cells and transmission in the hamster model and identify host factors affecting entry of SARS-CoV-2 in a genome-wide CRISPR screen.
- Yunkai Zhu
- , Fei Feng
- & Rong Zhang
-
Article
| Open AccessHigh resolution ensemble description of metamorphic and intrinsically disordered proteins using an efficient hybrid parallel tempering scheme
Mapping free energy landscapes of complex multi-funneled metamorphic proteins and weakly-funneled intrinsically disordered proteins (IDPs) remains challenging. Here authors present a parallel-tempering method that takes advantage of accelerated water dynamics for efficient and accurate conformational sampling across a wide variety of proteins.
- Rajeswari Appadurai
- , Jayashree Nagesh
- & Anand Srivastava
-
Article
| Open AccessAddressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope
Single-molecule fluorescence currently requires specialized imaging equipment due to the low signal of a single emitter. Here the authors introduce NanoAntennas with Cleared HOtSpots (NACHOS) to boost the signal sufficient for detection of a single emitter by a smartphone, opening the door to point-of-care applications.
- Kateryna Trofymchuk
- , Viktorija Glembockyte
- & Philip Tinnefeld
-
Article
| Open AccessActive methanogenesis during the melting of Marinoan snowball Earth
The deglaciation of Marinoan snowball Earth (~635 Myr ago) has been associated with potentially extensive CH4 emissions in relation to transient marine euxinia. Here, the authors find that active methanogenesis occurred during the termination of Marinoan snowball Earth, fueled by methyl sulfide production in sulfidic seawater.
- Zhouqiao Zhao
- , Bing Shen
- & Haoran Ma
-
Article
| Open AccessGWAS for autoimmune Addison’s disease identifies multiple risk loci and highlights AIRE in disease susceptibility
Autoimmune Addison’s disease is a rare complex disease, which has not yet been characterized by non-biased genetic studies. Here, the authors perform the first GWAS for the disease, identifying nine loci including two coding variants in the gene Autoimmune Regulator (AIRE).
- Daniel Eriksson
- , Ellen Christine Røyrvik
- & Eystein Sverre Husebye
-
Article
| Open AccessMethodological quality of COVID-19 clinical research
During the early phase of the COVID-19 pandemic there was a need for rapid dissemination of clinical findings. Here, Jung, Di Santo et al. perform a systematic review and cohort study providing evidence for lower methodological quality scores and faster time to publication of clinical studies related to COVID-19 than comparable studies.
- Richard G. Jung
- , Pietro Di Santo
- & Benjamin Hibbert
-
Article
| Open AccessAutomatic deep learning-driven label-free image-guided patch clamp system
Patch clamp recording of neurons is slow and labor-intensive. Here the authors present a method for automated deep learning driven label-free image guided patch clamp physiology to perform measurements on hundreds of human and rodent neurons.
- Krisztian Koos
- , Gáspár Oláh
- & Peter Horvath
-
Article
| Open AccessLETR1 is a lymphatic endothelial-specific lncRNA governing cell proliferation and migration through KLF4 and SEMA3C
Long noncoding RNAs regulate tissue-specific gene expression. Here the authors profile lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs) and show a role of LEC-specific lncRNA, LETR1, in cell proliferation and migration.
- Luca Ducoli
- , Saumya Agrawal
- & Michael Detmar
-
Article
| Open AccessMutant-selective degradation by BRAF-targeting PROTACs
Hundreds of BRAF mutations have been identified in patients with cancer but currently approved drugs only target BRAF V600 mutants. Here, the authors develop a vemurafenib-based PROTAC that induces degradation of all classes of BRAF mutants without affecting wild-type RAF proteins.
- Shanique Alabi
- , Saul Jaime-Figueroa
- & Craig M. Crews
-
Article
| Open AccessCryo-EM structures of engineered active bc1-cbb3 type CIII2CIV super-complexes and electronic communication between the complexes
Respiratory chains generate the proton motive force used for ATP synthesis. Cryo-EM structures of functional respiratory CIII2CIV supercomplex and native CIII2 from Rhodobacter capsulatus provide insight into CIII2CIV assembly and respiratory electron transport pathways in Gram-negative bacteria.
- Stefan Steimle
- , Trevor van Eeuwen
- & Fevzi Daldal
-
Review Article
| Open AccessThe metabolic impact of small intestinal nutrient sensing
The gastrointestinal tract participates in maintaining metabolic homeostasis in part through nutrient-sensing and subsequent gut-brain signalling. Here the authors review the role of small intestinal nutrient-sensing in regulation of energy intake and systemic glucose metabolism, and link high-fat diet, obesity and diabetes with perturbations in these pathways.
- Frank A. Duca
- , T. M. Zaved Waise
- & Tony K. T. Lam
-
Article
| Open AccessArachidonic acid-regulated calcium signaling in T cells from patients with rheumatoid arthritis promotes synovial inflammation
ORAI3 is part of pore forming calcium channels involved in T cell activation. Here the authors show that there is increased expression of ORAI3 in T cells from patients with rheumatoid arthritis and that the transcription factor IKAROS negatively regulates the ORAI3 promoter, indicating a regulatory loop that can control auto-reactivity of T cells in these patients.
- Zhongde Ye
- , Yi Shen
- & Jörg J. Goronzy
-
Article
| Open AccessPlasma cells are enriched in localized prostate cancer in Black men and are associated with improved outcomes
A recent report suggested Black men with prostate cancer were more responsive to immunotherapy. Here, the authors analysed prostate cancer gene expression profiles and show tumours from Black men and men with African ancestry have an increased proportion of plasma cells compared to those of White men and this correlates with improved outcome following treatment.
- Adam B. Weiner
- , Thiago Vidotto
- & Edward M. Schaeffer
-
Article
| Open AccessMultifunctional evolution of B and AGL6 MADS box genes in orchids
B class AP3/PI and AGL6-like MADS proteins determine lips and sepals/petals identities in orchids. Here, the authors characterize the extended function of OAP3/OPI/OAGL6 in regulating the specific structure of the lateral sepals, pigmentation/senescence of the perianth and abscission of the pedicel.
- Hsing-Fun Hsu
- , Wei-Han Chen
- & Chang-Hsien Yang
-
Article
| Open AccessFunctional and structural characterization of a flavoprotein monooxygenase essential for biogenesis of tryptophylquinone cofactor
An important type of post-translational protein modification is the conversion of peptidyl amino acid into enzyme cofactor. Here, the authors report functional and structural characterization of a flavoprotein monooxygenase essential for biosynthesis of cysteine tryptophylquinone (CTQ) cofactor.
- Toshinori Oozeki
- , Tadashi Nakai
- & Toshihide Okajima
-
Article
| Open AccessQuantitative single-protein imaging reveals molecular complex formation of integrin, talin, and kindlin during cell adhesion
Single-molecule localisation microscopy is limited by low labeling and detection efficiencies of the molecular probes. Here the authors report a framework to obtain absolute molecular quantities on a true molecular scale; the data reveal a ternary adhesion complex underlying cell-matrix adhesion.
- Lisa S. Fischer
- , Christoph Klingner
- & Carsten Grashoff
-
Article
| Open AccessDissociable roles of cortical excitation-inhibition balance during patch-leaving versus value-guided decisions
Here, the authors show that the balance between excitation and inhibition in two cortical areas is differentially related to maximizing immediate rewards, and to weighting the cost against long-term gains of moving to a new environment.
- Luca F. Kaiser
- , Theo O. J. Gruendler
- & Gerhard Jocham
-
Article
| Open AccessCavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation
Caveolae are spherical nanodomains of the plasma membrane generated by assembly of caveolin and cavin proteins. Here, the authors show that fuzzy electrostatic interactions between caveolin-1 and Cavin1 proteins, combined with membrane lipid interactions, are required to generate membrane curvature and a metastable caveola coat.
- Vikas A. Tillu
- , James Rae
- & Brett M. Collins
-
Article
| Open AccessGenetic determinants of daytime napping and effects on cardiometabolic health
The genetic basis of daytime napping and the directional effect of daytime napping on cardiometabolic health are unknown. Here, the authors perform a genome-wide association study on self-reported daytime napping in the UK Biobank and Mendelian randomization to explore causal associations.
- Hassan S. Dashti
- , Iyas Daghlas
- & Richa Saxena
Browse narrower subjects
- Biochemistry
- Biological techniques
- Biophysics
- Biotechnology
- Cancer
- Cell biology
- Chemical biology
- Computational biology and bioinformatics
- Developmental biology
- Drug discovery
- Ecology
- Evolution
- Genetics
- Immunology
- Microbiology
- Molecular biology
- Neuroscience
- Physiology
- Plant sciences
- Psychology
- Stem cells
- Structural biology
- Systems biology
- Zoology