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Cryo-EM structures of radial spokes and nexin−dynein regulatory complex (N-DRC) attached to doublet microtubules isolated from Chlamydomonas reinhardtii provide a model for mechanoregulation of motile cilia.
Cryo-EM analyses of the reconstituted radial spoke head and neck from Chlamydomonas reveal a rigid structure with an acidic surface, well-suited for communication with the microtubule central pair, and provide insight into human ciliopathies.
Cryo-EM and biochemical analyses of a subcomplex of S. cerevisiae RSC comprising its ATPase Sth1, Arp7, Arp9 and Rtt102 show that large conformational changes are part of Sth1 regulation and advance a mechanistic understanding of RSC complex assembly.
TET2 promotes the conversion of 5-methylcytosine to 5-hydroxymethylcytosine on tRNAs and affects the processing or stability of different classes of tRNA fragments.
Cryo-EM structures, along with transport assays with proteoliposomes and bacterial growth analyses, show that the Escherichiacoli Mla complex mediates ATP-dependent retrograde transport of phospholipids.
A solid-state NMR structure of the transmembrane domain from SARS-CoV-2 envelope protein in the phospholipid environment reveals determinants of cation selectivity, a dehydrated pore and an N-terminal drug-binding site.
Ultra-low-input native ChIP-seq analyses of genome-wide histone H3.3 distribution during early mouse development reveal a CAF-1-dependent transition from a non-canonical pattern in the oocyte and zygote to a canonical distribution at the two-cell stage.
Cryo-EM structures and functional analyses of wild-type and constitutively active Mec1–Ddc2 complexes reveal the basis of Mec1 kinase activation and how Dpb11 stimulates Mec1 activity.
Cryo-EM and liposome assays reveal that Atg9 functions as a lipid scramblase, transporting phospholipids between inner and outer liposome leaflets. Analyses of mutants in yeast support a role for this activity in autophagy.
Cryo-EM analyses together with liposome and cellular assays reveal that human ATG9A forms a trimer that mediates phospholipid flipping and promotes autophagosome membrane expansion.
A new cryo-EM structure of human DNA-PKcs in complex with a Ku70/80 heterodimer and DNA reveals how Ku80–DNA-PKcs interactions create a scaffold to mediate DNA double-strand break repair.
A directed genetic screen of chromatin regulators reveals that histone H3K4me2 deposition facilitates recovery of protein biosynthesis following UV-induced DNA damage repair and extends lifespan in C. elegans.
Proteomic definition of the telomeric PARylome combined with genetic and histone H3.3 deposition assays at telomeres reveals that PAR-regulated HIRA activity compensates for loss of ATRX in ALT cells.
A new X-ray crystal structure and supporting biochemical analyses of the human cardiac calsequestrin polymer reveal the basis of filament assembly and map disease-associated mutations to the multimerization interface.
The solid-state NMR structure of an amyloid fiber formed by human hormone β-endorphin reveals a protonated Glu8 in the fibrillar core. Under neutral conditions, deprotonation of Glu8 would contribute to fibril disassembly and hormone peptide release.
Primary cilia are isolated from monolayers of mammalian cells in culture for cryo-ET analyses, revealing new features as well as how they differ from motile cilia.
The effects of four antibodies on the aggregation pathway of Aβ are examined via an in-depth kinetics approach, revealing the specific molecular steps affected by each antibody.
Atomic force microscopy imaging of yeast condensin indicates that condensin may extrude DNA by switching conformation between open O and collapsed B shapes, indicative of a type of scrunching model.
Design of a bicistronic biosensor allows simultaneous quantification of cap- and IRES-mediated translation under normal and stress conditions in living mammalian cells.
Live-cell imaging of tagged genomic loci reveals clusters of distal enhancers in close proximity to target genes, creating a nanoenvironment wherein site-specifically recruited enhancer-associated regulatory factors activate transcription.