Volume 9 Issue 4, April 2014

Haploid Arabidopsis plants are easy to generate and have small, narrow leaves. The protocol by Wijnker et al. (doi:10.1038/nprot.2014.049) describes how (haploid) chromosome substitution lines (CSLs) are generated from Arabidopsis heterozygotes during reverse breeding. These CSLs subsequently serve as parental lines to recreate the heterozygote.