Volume 3 Issue 11, November 2008

Retinal vasculature visualized by direct DiI labeling. A flat-mounted retina was viewed by fluorescence microscopy. The microphotograph shows a retinal artery with its branches. The nuclei of endothelial cells are identifiable as oval spots. Image is from the protocol by Li et al.

Protocol

Superoxide radical detection in cells, tissues, organisms (animals, plants, insects, microorganisms) and soils

Christos D Georgiou
Ioannis Papapostolou
Konstantinos Grintzalis
Protocol 09 Oct 2008
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Visualization of ternary complexes in living cells by using a BiFC-based FRET assay

Y John Shyu
Christopher D Suarez
Chang-Deng Hu
Protocol 09 Oct 2008
Direct labeling and visualization of blood vessels with lipophilic carbocyanine dye DiI

Yiwen Li
Ying Song
Rong Wen
Protocol 09 Oct 2008
Methods for analyzing peptides and proteins on a chromatographic timescale by electron-transfer dissociation mass spectrometry

Namrata D Udeshi
Philip D Compton
Kristie L Rose
Protocol 16 Oct 2008
A rapid Percoll gradient procedure for preparation of synaptosomes

Peter R Dunkley
Paula E Jarvie
Phillip J Robinson
Protocol 16 Oct 2008
One-way PCR-based mapping of a replication initiation point (RIP)

Julia Romero
Hoyun Lee
Protocol 16 Oct 2008
DNA cleavage assay for the identification of topoisomerase I inhibitors

Thomas S Dexheimer
Yves Pommier
Protocol 16 Oct 2008
Positional cloning by fast-track SNP-mapping in Drosophila melanogaster

Frank Schnorrer
Annika Ahlford
Ann-Christine Syvänen
Protocol 23 Oct 2008
E-clonal antibodies: selection of full-length IgG antibodies using bacterial periplasmic display

Yariv Mazor
Thomas Van Blarcom
George Georgiou
Protocol 23 Oct 2008
Enzymatic assay for total plasma Cys

Qinghong Han
Robert M Hoffman
Protocol 23 Oct 2008
Gel chromatography and analytical ultracentrifugation to determine the extent of detergent binding and aggregation, and Stokes radius of membrane proteins using sarcoplasmic reticulum Ca²⁺–ATPase as an example

Marc le Maire
Bertrand Arnou
Jesper V Møller
Protocol 30 Oct 2008
Reverse-phase protein lysate microarrays for cell signaling analysis

Brett Spurrier
Sundhar Ramalingam
Satoshi Nishizuka
Protocol 30 Oct 2008
Calibrating excitation light fluxes for quantitative light microscopy in cell biology

David Grünwald
Shailesh M Shenoy
Robert H Singer
Protocol 30 Oct 2008

Volume 3 Issue 11, November 2008

Protocol

Superoxide radical detection in cells, tissues, organisms (animals, plants, insects, microorganisms) and soils

Visualization of ternary complexes in living cells by using a BiFC-based FRET assay

Direct labeling and visualization of blood vessels with lipophilic carbocyanine dye DiI

Methods for analyzing peptides and proteins on a chromatographic timescale by electron-transfer dissociation mass spectrometry

A rapid Percoll gradient procedure for preparation of synaptosomes

One-way PCR-based mapping of a replication initiation point (RIP)

DNA cleavage assay for the identification of topoisomerase I inhibitors

Positional cloning by fast-track SNP-mapping in Drosophila melanogaster

E-clonal antibodies: selection of full-length IgG antibodies using bacterial periplasmic display

Enzymatic assay for total plasma Cys

Gel chromatography and analytical ultracentrifugation to determine the extent of detergent binding and aggregation, and Stokes radius of membrane proteins using sarcoplasmic reticulum Ca²⁺–ATPase as an example

Reverse-phase protein lysate microarrays for cell signaling analysis

Calibrating excitation light fluxes for quantitative light microscopy in cell biology

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