Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
In their Perspective, Schubert et al. discuss developments and challenges in mass-spectrometry-based proteomics technology in the past decade and explore its role in molecular systems biology, clinical research and personalized medicine.
This Perspective discusses the development and applications of light-sheet-based fluorescence microscopy, a method with considerable advantages over confocal fluorescence microscopy.
In the last decade, super-resolution microscopy has enabled us to image cells with unprecedented detail. This Perspective discusses how the field can move beyond images to extract quantitative biological information from multidimensional SMLM data.
Single-particle cryo-electron microscopy has enabled the structures of large proteins to be elucidated. This Perspective discusses technological improvements in this technique, focusing particularly on the past decade and likely future developments.
In this Perspective, Elaine Mardis reviews a decade of DNA sequencing technology, from the introduction of Next-Generation Sequencing to single-molecule sequencing, including future applications that promise to further biological and biomedical research.