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This protocol extension describes the GFP thermal shift assay for monitoring ligand interactions of solute carrier transporters using either crude detergent-solubilized membranes or purified samples.
This Protocol Extension discusses several approaches to analyzing clonogenic growth of mammalian cells in vitro, using a modular framework to facilitate the use of various formats to fully optimize clonogenic growth.
The authors present a protocol for testing physiologically relevant infection conditions (e.g., lung and wound exudate or blood) in minimal inhibitory concentration (MIC) assays.
This extension of a previous in vitro digestion protocol provides a subsequent in vitro batch fermentation stage that is carried out afterward to enable investigation of the effect of food on the gut microbiome.
Here, we present a protocol for high-throughput screening of SPOT-peptide arrays to assess the antibiofilm, antimicrobial and immunomodulatory activities of synthetic peptides.
This Protocol Extension details the use of zebrafish larvae as a simple and robust in vivo system for studying human norovirus infection, enabling evaluation of the antiviral effects and toxicities of small molecules.
This protocol describes how to direct differentiation of human pluripotent stem cells in a 3D matrix of collagen I to cultures containing mature alveolar type II and I cells plus airway basal, ciliated, club and neuroendocrine cells.
In this extension to their NET-seq protocol, the authors combine isolation of 4sU-labeled chromatin-associated nascent RNA with long-read direct RNA sequencing on nanopores to profile the kinetics and patterns of co-transcriptional RNA processing.
This Protocol Extension presents recombinant extracellular vesicles as reference materials for method development and standardization. It details their characterization and detection in spiked samples by fluorescence, nucleic acid and protein measurements.