News & Views

A combined experimental and computational approach to transcriptomic profiling of cell ‘multiplets’ enables the reconstruction of cell–cell interactions and higher-order structural features of biological tissues.

Co-fractionation mass spectrometry (CF-MS) has the potential to measure thousands of protein complexes in a single experiment, but the field is still in its infancy. A meta-analysis of CF-MS data yields a core CF-MS interactome and a tool allowing researchers to align new results to published data.

A study applies polymer physics to assess the advantages and limitations of three sequencing-based approaches for determining the structure of genomes and genomic domains.

Light-field microscopes can image three-dimensional dynamics of biological samples at unprecedented speed, but the computational reconstruction necessary for image formation is artifact-prone and time-consuming. Deep learning closes this gap between imaging and reconstruction speed.

Two new technologies enable the profiling of single cells for RNA transcription as well as modifications to histone proteins. Each takes a similar strategy to capture both properties, but with different methods for cell indexing, and they are applied to two different areas: neuroscience and developmental biology, respectively.

CEPT, a small-molecule cocktail, improves the viability of human pluripotent stem cells, protects cells during culture and cryopreservation, and promotes in vitro differentiation and organoid formation.

With protein structure prediction recently getting a seismic boost in accuracy, hopes are also up to better predict unstructured protein regions that can adopt diverse conformations. CAID, a community effort to revive systematic benchmarking, should help.

A new approach tracks animal movements in 3D from multiple camera views using volumetric triangulation, reconciling occlusions and ambiguities present in any one camera view.

Souza et al. present the latest release of the popular and widely used force field for near-atomistic coarse-grained biomolecular simulations.

Single-molecule FRET is making stepwise progress toward the realization of its full potential: becoming the reference technique to monitor protein structural dynamics in live cells.

A genetically encoded neurotransmitter sensor, engineered from a tick lipocalin, detects serotonin in the living brain.

In vacuum, a monolayer graphene cover enables imaging mass spectrometry of living, wet cells.

A community-wide challenge yields recommendations for improving cryo-EM structure validation.

Algorithms from the Nesvizhskii and Smith groups facilitate faster and more comprehensive glycopeptide assignments from mass spectrometry datasets.

Plankton regularly travel vast distances up and down in the ocean. A water-filled hamster wheel with glass windows now enables detailed microscopic lab observations of individual aquatic microorganisms during their vertical migrations.

Producing reliable atomic- or close-to-atomic-resolution structures of RNA-only molecules has been a formidable task. Ribosolve can solve sub-nanometer-resolution cryo-EM structures of unbound RNA molecules with unprecedented accuracy and speed.

A resource of detailed DNA delivery and expression protocols for marine protists will enable new studies to understand the fundamental and ancestral features of eukaryotic cells.

New methods from the Heo and Johnsson labs enable light- and drug-inducible control of intrabody binding in cells.

In two novel RNA modification mapping methods, the authors have engineered RNA enzymes and used the enzyme-mediated mutational signatures to map m⁶A and m¹A at single-nucleotide resolution in mammalian RNA.