Articles in 2020

  • Article
    | Open Access

    Surprisingly, motor cortex becomes less involved in performing skilled motor behaviors as they are practiced. This is addressed by a model of two descending pathways featuring different types of learning: fast learning in a cortical pathway to maximize rewards and slow learning in a subcortical pathway to reinforce behaviors through repetition.

    • James M. Murray
    •  & G. Sean Escola
  • Article
    | Open Access

    Dihydrobenzofurans and indolines are common substructures in medicines and natural products. Herein, the authors report a palladium-catalyzed [3 + 2] (hetero)annulation proceeding in an anti-selective fashion and enabling direct access to these valuable heterocycles with broad reaction scope.

    • Hui-Qi Ni
    • , Ilia Kevlishvili
    • , Pranali G. Bedekar
    • , Joyann S. Barber
    • , Shouliang Yang
    • , Michelle Tran-Dubé
    • , Andrew M. Romine
    • , Hou-Xiang Lu
    • , Indrawan J. McAlpine
    • , Peng Liu
    •  & Keary M. Engle
  • Perspective
    | Open Access

    Ketamine is associated with rapid antidepressant effects and temporary dissociative experiences, and this review examines whether these dissociative symptoms are necessary for antidepressant efficacy. Although the current literature does not support this relationship, further work is needed to explore possible associations at the molecular, biomarker, and psychological levels.

    • Elizabeth D. Ballard
    •  & Carlos A. Zarate Jr.
  • Article
    | Open Access

    β-tryptases are responsible for most of the proteolytic activity during mast cell activation. Here, the authors develop β-tryptase-inhibiting antibodies and provide structural and biochemical evidence that the bivalency of the antibodies is a prerequisite for their inhibitory activity.

    • Henry R. Maun
    • , Rajesh Vij
    • , Benjamin T. Walters
    • , Ashley Morando
    • , Janet K. Jackman
    • , Ping Wu
    • , Alberto Estevez
    • , Xiaocheng Chen
    • , Yvonne Franke
    • , Michael T. Lipari
    • , Mark S. Dennis
    • , Daniel Kirchhofer
    • , Claudio Ciferri
    • , Kelly M. Loyet
    • , Tangsheng Yi
    • , Charles Eigenbrot
    • , Robert A. Lazarus
    •  & James T. Koerber
  • Article
    | Open Access

    The navigation of catheters through blood vessels requires flexible guiding wires that are pushable and tractable at the same time. Pancaldi et al. rely on hydrodynamic forces and magnetic torque in order to access even rather small capillaries with an ultraflexible magnetomechanical probe.

    • Lucio Pancaldi
    • , Pietro Dirix
    • , Adele Fanelli
    • , Augusto Martins Lima
    • , Nikolaos Stergiopulos
    • , Pascal John Mosimann
    • , Diego Ghezzi
    •  & Mahmut Selman Sakar
  • Article
    | Open Access

    The dopamine D2 receptor (D2R) is a GPCR and an important drug target for schizophrenia treatment. Here, the authors present the crystal structure of human D2R in complex with the antipsychotic drug spiperone, which is of interest for designing antipsychotics with improved receptor selectivity.

    • Dohyun Im
    • , Asuka Inoue
    • , Takaaki Fujiwara
    • , Takanori Nakane
    • , Yasuaki Yamanaka
    • , Tomoko Uemura
    • , Chihiro Mori
    • , Yuki Shiimura
    • , Kanako Terakado Kimura
    • , Hidetsugu Asada
    • , Norimichi Nomura
    • , Tomoyuki Tanaka
    • , Ayumi Yamashita
    • , Eriko Nango
    • , Kensuke Tono
    • , Francois Marie Ngako Kadji
    • , Junken Aoki
    • , So Iwata
    •  & Tatsuro Shimamura
  • Article
    | Open Access

    A group of long non-coding RNAs (lncRNAs), Mammary Tumor Associated RNAs 1-30 (MaTARs 1-30), are differentially expressed between mammary tumor cells and normal mammary epithelial cells. Here the authors report that MaTAR25 plays a role in breast cancer cell proliferation, migration and invasion by regulating the expression of the Tensin1 gene in trans.

    • Kung-Chi Chang
    • , Sarah D. Diermeier
    • , Allen T. Yu
    • , Lily D. Brine
    • , Suzanne Russo
    • , Sonam Bhatia
    • , Habeeb Alsudani
    • , Karen Kostroff
    • , Tawfiqul Bhuiya
    • , Edi Brogi
    • , Darryl J. Pappin
    • , C. Frank Bennett
    • , Frank Rigo
    •  & David L. Spector
  • Article
    | Open Access

    Design and discovery of catalysts to make clean energy and mitigate the harmful effect of greenhouse gases remains a massive challenge. Here the authors report a combustion-synthesised iron-based catalyst of high activity and selectivity for directly converting CO2 to aviation jet fuel.

    • Benzhen Yao
    • , Tiancun Xiao
    • , Ofentse A. Makgae
    • , Xiangyu Jie
    • , Sergio Gonzalez-Cortes
    • , Shaoliang Guan
    • , Angus I. Kirkland
    • , Jonathan R. Dilworth
    • , Hamid A. Al-Megren
    • , Saeed M. Alshihri
    • , Peter J. Dobson
    • , Gari P. Owen
    • , John M. Thomas
    •  & Peter P. Edwards
  • Article
    | Open Access

    Chlamydia trachomatis does not possess TrpL-mediated transcription attenuation of its trp operon. Here, the authors show that an iron-dependent regulator, YtgR, acts as a tryptophan-dependent attenuator of the trp operon in this organism, due to translational regulation of YtgR levels via a triple tryptophan motif.

    • Nick D. Pokorzynski
    • , Nathan D. Hatch
    • , Scot P. Ouellette
    •  & Rey A. Carabeo
  • Article
    | Open Access

    Designing efficient blue perovskite LEDs by using mixed halides perovskite is still a challenge, limited mainly by the phase segregation issue. Here, the authors demonstrate in situ fabrication of quasi-2D CsPbClBr2 nanocrystal films with mixed ligands to overcome the constraint.

    • Chenhui Wang
    • , Dengbao Han
    • , Junhui Wang
    • , Yingguo Yang
    • , Xinyue Liu
    • , Sheng Huang
    • , Xin Zhang
    • , Shuai Chang
    • , Kaifeng Wu
    •  & Haizheng Zhong
  • Article
    | Open Access

    Using Chang’E data, the authors here identify more than 109,000 previously unrecognized lunar craters and date almost 19,000 craters based on transfer learning with deep neural networks. A new lunar crater database is derived and distributed to the planetary community.

    • Chen Yang
    • , Haishi Zhao
    • , Lorenzo Bruzzone
    • , Jon Atli Benediktsson
    • , Yanchun Liang
    • , Bin Liu
    • , Xingguo Zeng
    • , Renchu Guan
    • , Chunlai Li
    •  & Ziyuan Ouyang
  • Article
    | Open Access

    The vertebrate TATA-binding protein (TBP) paralogue (TBPL2) is only expressed in growing oocytes, where TBP is absent. Here the authors highlight a unique role for the TBPL2/TFIIA complex in the establishment of the oocyte transcriptome by using a specific TSS recognition code.

    • Changwei Yu
    • , Nevena Cvetesic
    • , Vincent Hisler
    • , Kapil Gupta
    • , Tao Ye
    • , Emese Gazdag
    • , Luc Negroni
    • , Petra Hajkova
    • , Imre Berger
    • , Boris Lenhard
    • , Ferenc Müller
    • , Stéphane D. Vincent
    •  & László Tora
  • Article
    | Open Access

    The actin-related protein (Arp)2/3 complex nucleates branched actin filament networks pivotal for cell migration, endocytosis and pathogen infection. Here, authors report a 9.0 Å resolution structure of the actin filament Arp2/3 complex branch junction in cells using cryo-electron tomography and subtomogram averaging.

    • Florian Fäßler
    • , Georgi Dimchev
    • , Victor-Valentin Hodirnau
    • , William Wan
    •  & Florian K. M. Schur
  • Article
    | Open Access

    IL-2/anti-IL-2 complexes have been proposed to curtail the severe adverse effects associated with IL-2 immunotherapy. Here, the authors, by integrating unmutated human IL-2 in the antigen binding groove of an anti-IL-2 monoclonal antibody, generate a CD122-biased fusion protein that prevents binding of IL-2 to CD25 and promotes anti-tumor immune response in several preclinical metastatic cancer models.

    • Dilara Sahin
    • , Natalia Arenas-Ramirez
    • , Matthias Rath
    • , Ufuk Karakus
    • , Monika Hümbelin
    • , Merel van Gogh
    • , Lubor Borsig
    •  & Onur Boyman
  • Article
    | Open Access

    Molecular profiling of breast cancer in non-Caucasian populations remains underexplored. Here the authors report a high prevalence of HER2-subtypes and enriched immune score with improved survival and higher rates of TP53 somatic mutations with poorer survival in ER+ tumours in a Malaysian cohort.

    • Jia-Wern Pan
    • , Muhammad Mamduh Ahmad Zabidi
    • , Pei-Sze Ng
    • , Mei-Yee Meng
    • , Siti Norhidayu Hasan
    • , Bethan Sandey
    • , Stephen-John Sammut
    • , Cheng-Har Yip
    • , Pathmanathan Rajadurai
    • , Oscar M. Rueda
    • , Carlos Caldas
    • , Suet-Feung Chin
    •  & Soo-Hwang Teo
  • Article
    | Open Access

    The fungal pathogen Candida auris is resistant to multiple drugs including the common antifungal fluconazole. Here, Iyer et al. identify a compound that potentiates fluconazole activity against C. auris in vitro and in vivo by inhibiting a major efflux pump and thus increasing intracellular fluconazole accumulation.

    • Kali R. Iyer
    • , Kaddy Camara
    • , Martin Daniel-Ivad
    • , Richard Trilles
    • , Sheila M. Pimentel-Elardo
    • , Jen L. Fossen
    • , Karen Marchillo
    • , Zhongle Liu
    • , Shakti Singh
    • , José F. Muñoz
    • , Sang Hu Kim
    • , John A. Porco Jr.
    • , Christina A. Cuomo
    • , Noelle S. Williams
    • , Ashraf S. Ibrahim
    • , John E. Edwards Jr.
    • , David R. Andes
    • , Justin R. Nodwell
    • , Lauren E. Brown
    • , Luke Whitesell
    • , Nicole Robbins
    •  & Leah E. Cowen
  • Author Correction
    | Open Access

    • Antonio Molinaro
    • , Pierre Bel Lassen
    • , Marcus Henricsson
    • , Hao Wu
    • , Solia Adriouch
    • , Eugeni Belda
    • , Rima Chakaroun
    • , Trine Nielsen
    • , Per-Olof Bergh
    • , Christine Rouault
    • , Sébastien André
    • , Florian Marquet
    • , Fabrizio Andreelli
    • , Joe-Elie Salem
    • , Karen Assmann
    • , Jean-Philippe Bastard
    • , Sofia Forslund
    • , Emmanuelle Le Chatelier
    • , Gwen Falony
    • , Nicolas Pons
    • , Edi Prifti
    • , Benoit Quinquis
    • , Hugo Roume
    • , Sara Vieira-Silva
    • , Tue H. Hansen
    • , Helle Krogh Pedersen
    • , Christian Lewinter
    • , Nadja B. Sønderskov
    • , Renato Alves
    • , Chloe Amouyal
    • , Ehm Astrid Andersson Galijatovic
    • , Olivier Barthelemy
    • , Jean-Paul Batisse
    • , Magalie Berland
    • , Randa Bittar
    • , Hervé Blottière
    • , Frederic Bosquet
    • , Rachid Boubrit
    • , Olivier Bourron
    • , Mickael Camus
    • , Dominique Cassuto
    • , Julien Chilloux
    • , Cecile Ciangura
    • , Luis Pedro Coelho
    • , Jean-Philippe Collet
    • , Maria-Carlota Dao
    • , Morad Djebbar
    • , Angélique Doré
    • , Line Engelbrechtsen
    • , Soraya Fellahi
    • , Leopold Fezeu
    • , Sebastien Fromentin
    • , Philippe Giral
    • , Jens Peter Gøtze
    • , Agnes Hartemann
    • , Jens Juul Holst
    • , Serge Hercberg
    • , Gerard Helft
    • , Malene Hornbak
    • , Jean-Sebastien Hulot
    • , Richard Isnard
    • , Sophie Jaqueminet
    • , Niklas Rye Jørgensen
    • , Hanna Julienne
    • , Johanne Justesen
    • , Judith Kammer
    • , Nikolaj Krarup
    • , Mathieu Kerneis
    • , Jean Khemis
    • , Nadja Buus Kristensen
    • , Michael Kuhn
    • , Véronique Lejard
    • , Florence Levenez
    • , Lea Lucas-Martini
    • , Robin Massey
    • , Nicolas Maziers
    • , Jonathan Medina-Stamminger
    • , Gilles Montalescot
    • , Sandrine Moutel
    • , Laetitia Pasero Le Pavin
    • , Christine Poitou
    • , Francoise Pousset
    • , Laurence Pouzoulet
    • , Sebastien Schmidt
    • , Lucas Moitinho-Silva
    • , Johanne Silvain
    • , Nataliya Sokolovska
    • , Sothea Touch
    • , Mathilde Svendstrup
    • , Timothy Swartz
    • , Thierry Vanduyvenboden
    • , Camille Vatier
    • , Stefanie Walther
    • , Lars Køber
    • , Henrik Vestergaard
    • , Torben Hansen
    • , Jean-Daniel Zucker
    • , Pilar Galan
    • , Marc-Emmanuel Dumas
    • , Jeroen Raes
    • , Jean-Michel Oppert
    • , Ivica Letunic
    • , Jens Nielsen
    • , Peer Bork
    • , S. Dusko Ehrlich
    • , Michael Stumvoll
    • , Oluf Pedersen
    • , Judith Aron-Wisnewsky
    • , Karine Clément
    •  & Fredrik Bäckhed
  • Editorial
    | Open Access

    The publication of a paper on mentorship, now retracted, led us to reflect on our editorial processes and strengthened our determination in supporting diversity, equity and inclusion in research.

  • Article
    | Open Access

    Pre conditioning injury or environmental enrichment have been shown to promote axon regeneration. Here the authors show that environmental enrichment, combined with preconditioning injury promotes regeneration via a redox signalling dependent mechanism.

    • Francesco De Virgiliis
    • , Thomas H. Hutson
    • , Ilaria Palmisano
    • , Sarah Amachree
    • , Jian Miao
    • , Luming Zhou
    • , Rositsa Todorova
    • , Richard Thompson
    • , Matt C. Danzi
    • , Vance P. Lemmon
    • , John L. Bixby
    • , Ilka Wittig
    • , Ajay M. Shah
    •  & Simone Di Giovanni
  • Article
    | Open Access

    Arrays of ordered metal atoms on bulk insulating materials are promising for future applications, such as optoelectronics and data storage. Here, the authors demonstrate a strategy to create an ordered metal array based on tailored anchoring and hard-sphere repulsion of metal-complexing molecules.

    • Simon Aeschlimann
    • , Sebastian V. Bauer
    • , Maximilian Vogtland
    • , Benjamin Stadtmüller
    • , Martin Aeschlimann
    • , Andrea Floris
    • , Ralf Bechstein
    •  & Angelika Kühnle
  • Article
    | Open Access

    The causes and consequences of social intelligence are challenging to establish. A study on wild cleaner fish reports that large forebrains enable individuals to score higher in a social competence test, suggesting forebrain size is important for complex social decision-making.

    • Zegni Triki
    • , Yasmin Emery
    • , Magda C. Teles
    • , Rui F. Oliveira
    •  & Redouan Bshary
  • Article
    | Open Access

    Intestinal cancer stem cells (CSC) are associated with colon cancer. Here, the authors show that Wnt/beta-catenin signalling in CSC requires the epigenetic regulator Mll1 to promote stemness and tumourigenesis in murine and human colon cancer models.

    • Johanna Grinat
    • , Julian Heuberger
    • , Ramon Oliveira Vidal
    • , Neha Goveas
    • , Frauke Kosel
    • , Antoni Berenguer-Llergo
    • , Andrea Kranz
    • , Annika Wulf-Goldenberg
    • , Diana Behrens
    • , Bálint Melcher
    • , Sascha Sauer
    • , Michael Vieth
    • , Eduard Batlle
    • , A. Francis Stewart
    •  & Walter Birchmeier
  • Article
    | Open Access

    The bacterial helicase-like transcription factor HelD associates with the RNA polymerase (RNAP) and recycles stalled transcription complexes. Here, the authors present the cryo-EM structures of three Mycobacterium smegmatis HelD bound RNAP complexes and further show that HelD can prevent the binding of the RNAP core to non-specific DNA and also actively removes RNAP from stalled elongation complexes.

    • Tomáš Kouba
    • , Tomáš Koval’
    • , Petra Sudzinová
    • , Jiří Pospíšil
    • , Barbora Brezovská
    • , Jarmila Hnilicová
    • , Hana Šanderová
    • , Martina Janoušková
    • , Michaela Šiková
    • , Petr Halada
    • , Michal Sýkora
    • , Ivan Barvík
    • , Jiří Nováček
    • , Mária Trundová
    • , Jarmila Dušková
    • , Tereza Skálová
    • , URee Chon
    • , Katsuhiko S. Murakami
    • , Jan Dohnálek
    •  & Libor Krásný
  • Article
    | Open Access

    The authors present a method for calculating the accuracy of an NMR structure, where flexibility from backbone chemical shifts is compared to structural flexibility predicted using rigidity theory. The authors validate their method and use it to compare the accuracy of NMR and X-ray structures.

    • Nicholas J. Fowler
    • , Adnan Sljoka
    •  & Mike P. Williamson
  • Article
    | Open Access

    Schistosomes undergo several develepmental stages during infection of humans. Here, the authors perform single-cell RNA sequencing on the earliest intra-mammalian stage of Schistosoma mansoni and generate a comprehensive cell-type atlas for this human parasite.

    • Carmen Lidia Diaz Soria
    • , Jayhun Lee
    • , Tracy Chong
    • , Avril Coghlan
    • , Alan Tracey
    • , Matthew D. Young
    • , Tallulah Andrews
    • , Christopher Hall
    • , Bee Ling Ng
    • , Kate Rawlinson
    • , Stephen R. Doyle
    • , Steven Leonard
    • , Zhigang Lu
    • , Hayley M. Bennett
    • , Gabriel Rinaldi
    • , Phillip A. Newmark
    •  & Matthew Berriman
  • Article
    | Open Access

    The bacterial helicase-like transcription factor HelD interacts with the RNA polymerase (RNAP) and together with the RNAP δ subunit enhances RNAP cycling. Here, the authors present the cryo-EM structures of the monomeric and dimeric Bacillus subtilis RNAP-δ-HelD complexes and suggest a model for HelD/δ-mediated RNAP recycling and putative hibernation.

    • Hao-Hong Pei
    • , Tarek Hilal
    • , Zhuo A. Chen
    • , Yong-Heng Huang
    • , Yuan Gao
    • , Nelly Said
    • , Bernhard Loll
    • , Juri Rappsilber
    • , Georgiy A. Belogurov
    • , Irina Artsimovitch
    •  & Markus C. Wahl
  • Article
    | Open Access

    Drugs targeting cardiovascular disease (CVD) can have negative consequences for liver function. Here, the authors combine genome wide analyses on 69,479 individuals to identify loss-of-function variants with beneficial effects on CVD-related traits without negative impacts on liver function.

    • Jonas B. Nielsen
    • , Oren Rom
    • , Ida Surakka
    • , Sarah E. Graham
    • , Wei Zhou
    • , Tanmoy Roychowdhury
    • , Lars G. Fritsche
    • , Sarah A. Gagliano Taliun
    • , Carlo Sidore
    • , Yuhao Liu
    • , Maiken E. Gabrielsen
    • , Anne Heidi Skogholt
    • , Brooke Wolford
    • , William Overton
    • , Ying Zhao
    • , Jin Chen
    • , He Zhang
    • , Whitney E. Hornsby
    • , Akua Acheampong
    • , Austen Grooms
    • , Amanda Schaefer
    • , Gregory J. M. Zajac
    • , Luis Villacorta
    • , Jifeng Zhang
    • , Ben Brumpton
    • , Mari Løset
    • , Vivek Rai
    • , Pia R. Lundegaard
    • , Morten S. Olesen
    • , Kent D. Taylor
    • , Nicholette D. Palmer
    • , Yii-Der Chen
    • , Seung H. Choi
    • , Steven A. Lubitz
    • , Patrick T. Ellinor
    • , Kathleen C. Barnes
    • , Michelle Daya
    • , Nicholas Rafaels
    • , Scott T. Weiss
    • , Jessica Lasky-Su
    • , Russell P. Tracy
    • , Ramachandran S. Vasan
    • , L. Adrienne Cupples
    • , Rasika A. Mathias
    • , Lisa R. Yanek
    • , Lewis C. Becker
    • , Patricia A. Peyser
    • , Lawrence F. Bielak
    • , Jennifer A. Smith
    • , Stella Aslibekyan
    • , Bertha A. Hidalgo
    • , Donna K. Arnett
    • , Marguerite R. Irvin
    • , James G. Wilson
    • , Solomon K. Musani
    • , Adolfo Correa
    • , Stephen S. Rich
    • , Xiuqing Guo
    • , Jerome I. Rotter
    • , Barbara A. Konkle
    • , Jill M. Johnsen
    • , Allison E. Ashley-Koch
    • , Marilyn J. Telen
    • , Vivien A. Sheehan
    • , John Blangero
    • , Joanne E. Curran
    • , Juan M. Peralta
    • , Courtney Montgomery
    • , Wayne H-H Sheu
    • , Ren-Hua Chung
    • , Karen Schwander
    • , Seyed M. Nouraie
    • , Victor R. Gordeuk
    • , Yingze Zhang
    • , Charles Kooperberg
    • , Alexander P. Reiner
    • , Rebecca D. Jackson
    • , Eugene R. Bleecker
    • , Deborah A. Meyers
    • , Xingnan Li
    • , Sayantan Das
    • , Ketian Yu
    • , Jonathon LeFaive
    • , Albert Smith
    • , Tom Blackwell
    • , Daniel Taliun
    • , Sebastian Zollner
    • , Lukas Forer
    • , Sebastian Schoenherr
    • , Christian Fuchsberger
    • , Anita Pandit
    • , Matthew Zawistowski
    • , Sachin Kheterpal
    • , Chad M. Brummett
    • , Pradeep Natarajan
    • , David Schlessinger
    • , Seunggeun Lee
    • , Hyun Min Kang
    • , Francesco Cucca
    • , Oddgeir L. Holmen
    • , Bjørn O. Åsvold
    • , Michael Boehnke
    • , Sekar Kathiresan
    • , Goncalo R. Abecasis
    • , Y. Eugene Chen
    • , Cristen J. Willer
    •  & Kristian Hveem
  • Article
    | Open Access

    Glioblastoma is divided into four subtypes based on molecular profiling at the methylome and transcriptome level. Here the authors perform an integrative analysis of these subtypes resulting in the identification of SOX10 whose loss induces a mesenchymal phenotype and promotes tumour progression.

    • Yonghe Wu
    • , Michael Fletcher
    • , Zuguang Gu
    • , Qi Wang
    • , Barbara Costa
    • , Anna Bertoni
    • , Ka-Hou Man
    • , Magdalena Schlotter
    • , Jörg Felsberg
    • , Jasmin Mangei
    • , Martje Barbus
    • , Ann-Christin Gaupel
    • , Wei Wang
    • , Tobias Weiss
    • , Roland Eils
    • , Michael Weller
    • , Haikun Liu
    • , Guido Reifenberger
    • , Andrey Korshunov
    • , Peter Angel
    • , Peter Lichter
    • , Carl Herrmann
    •  & Bernhard Radlwimmer
  • Article
    | Open Access

    Ancient, asexual lineages are rare as a lack of recombination is usually an evolutionary dead end. Here, authors compare complete genomes of 11 individual bdelloid rotifers that suggest evidence of regular genetic exchange between individuals in a species that was previously thought to be asexual.

    • Olga A. Vakhrusheva
    • , Elena A. Mnatsakanova
    • , Yan R. Galimov
    • , Tatiana V. Neretina
    • , Evgeny S. Gerasimov
    • , Sergey A. Naumenko
    • , Svetlana G. Ozerova
    • , Arthur O. Zalevsky
    • , Irina A. Yushenova
    • , Fernando Rodriguez
    • , Irina R. Arkhipova
    • , Aleksey A. Penin
    • , Maria D. Logacheva
    • , Georgii A. Bazykin
    •  & Alexey S. Kondrashov
  • Article
    | Open Access

    Gram-positive bacteria contain a transcription factor HelD that is able to remove and recycle stalled transcription complexes. Here the authors provide mechanistic insights into this process by determining the cryo-EM structures of the Bacillus subtilis RNA polymerase (RNAP) elongation complex and the RNAP-HelD transcription recycling complex and propose a model of HelD catalysed transcription recycling.

    • Timothy P. Newing
    • , Aaron J. Oakley
    • , Michael Miller
    • , Catherine J. Dawson
    • , Simon H. J. Brown
    • , James C. Bouwer
    • , Gökhan Tolun
    •  & Peter J. Lewis
  • Author Correction
    | Open Access

    • Christopher B. Mulholland
    • , Atsuya Nishiyama
    • , Joel Ryan
    • , Ryohei Nakamura
    • , Merve Yiğit
    • , Ivo M. Glück
    • , Carina Trummer
    • , Weihua Qin
    • , Michael D. Bartoschek
    • , Franziska R. Traube
    • , Edris Parsa
    • , Enes Ugur
    • , Miha Modic
    • , Aishwarya Acharya
    • , Paul Stolz
    • , Christoph Ziegenhain
    • , Michael Wierer
    • , Wolfgang Enard
    • , Thomas Carell
    • , Don C. Lamb
    • , Hiroyuki Takeda
    • , Makoto Nakanishi
    • , Sebastian Bultmann
    •  & Heinrich Leonhardt
  • Article
    | Open Access

    Encasing a single atom within a fullerene (C60) cage can create a robustly packaged single atomic spin system. Here, the authors perform electron paramagnetic resonance on a single encased spin using a diamond NV-center, demonstrating the first steps in controlling single spins in fullerene cages.

    • Dinesh Pinto
    • , Domenico Paone
    • , Bastian Kern
    • , Tim Dierker
    • , René Wieczorek
    • , Aparajita Singha
    • , Durga Dasari
    • , Amit Finkler
    • , Wolfgang Harneit
    • , Jörg Wrachtrup
    •  & Klaus Kern
  • Article
    | Open Access

    Here, graphene-based plasmonic metamaterials are used to generate an artificial bulk photovoltaic effect, enabling the realization of mid-infrared photodetectors with enhanced responsivity and calibration-free polarization detection at room temperature.

    • Jingxuan Wei
    • , Ying Li
    • , Lin Wang
    • , Wugang Liao
    • , Bowei Dong
    • , Cheng Xu
    • , Chunxiang Zhu
    • , Kah-Wee Ang
    • , Cheng-Wei Qiu
    •  & Chengkuo Lee
  • Article
    | Open Access

    Promising results of cancer therapies in transplant tumor models often fail to predict efficacy in clinical trials. Here the authors show that, while transplant tumors are cured by radiotherapy and PD-1 blockade, autochthonous sarcomas are resistant to the identical treatment, recapitulating the immune landscape and resistance to checkpoint blockade observed in most sarcoma patients.

    • Amy J. Wisdom
    • , Yvonne M. Mowery
    • , Cierra S. Hong
    • , Jonathon E. Himes
    • , Barzin Y. Nabet
    • , Xiaodi Qin
    • , Dadong Zhang
    • , Lan Chen
    • , Hélène Fradin
    • , Rutulkumar Patel
    • , Alex M. Bassil
    • , Eric S. Muise
    • , Daniel A. King
    • , Eric S. Xu
    • , David J. Carpenter
    • , Collin L. Kent
    • , Kimberly S. Smythe
    • , Nerissa T. Williams
    • , Lixia Luo
    • , Yan Ma
    • , Ash A. Alizadeh
    • , Kouros Owzar
    • , Maximilian Diehn
    • , Todd Bradley
    •  & David G. Kirsch
  • Article
    | Open Access

    It is unclear whether body size affects community assembly mechanisms of soil biota. Here, the authors analyse soil microbial and nematode communities sampled along a 4000-km transect in China and global soil microbiome data to show that bacterial assembly is governed by high dispersal, whereas larger taxa are more influenced by deterministic processes.

    • Lu Luan
    • , Yuji Jiang
    • , Menghua Cheng
    • , Francisco Dini-Andreote
    • , Yueyu Sui
    • , Qinsong Xu
    • , Stefan Geisen
    •  & Bo Sun
  • Article
    | Open Access

    Muscle cells express an adhesion molecule called metavinculin, which has been associated with cardiomyopathies. Here, the authors employed molecular tension sensors to reveal that metavinculin expression modulates cell adhesion mechanics and they develop a mouse model to demonstrate that the presence of metavinculin is not as critical for heart muscle function as previously thought.

    • Verena Kanoldt
    • , Carleen Kluger
    • , Christiane Barz
    • , Anna-Lena Schweizer
    • , Deepak Ramanujam
    • , Lukas Windgasse
    • , Stefan Engelhardt
    • , Anna Chrostek-Grashoff
    •  & Carsten Grashoff
  • Article
    | Open Access

    The eukaryotic RNA Polymerase III transcribes tRNAs, some ribosomal and spliceosomal RNAs. Here, the authors resolve a cryo-EM structure of human RNA Polymerase III in its apo form and complemented it with crystal structures and SAXS analysis of RPC5, revealing insights into the molecular mechanisms of Pol III transcription.

    • Ewan Phillip Ramsay
    • , Guillermo Abascal-Palacios
    • , Julia L. Daiß
    • , Helen King
    • , Jerome Gouge
    • , Michael Pilsl
    • , Fabienne Beuron
    • , Edward Morris
    • , Philip Gunkel
    • , Christoph Engel
    •  & Alessandro Vannini
  • Article
    | Open Access

    Endocannabinoid levels are controlled by the fine balance between their synthesis and degradation. Here, the authors show that memory formation through fear conditioning selectively accelerates the degradation of endocannabinoids in the cerebellum via a lasting increase in GABA release.

    • Christophe J. Dubois
    • , Jessica Fawcett-Patel
    • , Paul A. Katzman
    •  & Siqiong June Liu
  • Article
    | Open Access

    Here, the authors generate a replication-competent VSV based vaccine expressing SARS-CoV-2 spike protein and show protection in the hamster model with one dose. Analysis of the antibody response in mice shows induction of neutralizing antibodies and suggests a desirable Th1-biased response to the vaccine.

    • Yfat Yahalom-Ronen
    • , Hadas Tamir
    • , Sharon Melamed
    • , Boaz Politi
    • , Ohad Shifman
    • , Hagit Achdout
    • , Einat B. Vitner
    • , Ofir Israeli
    • , Elad Milrot
    • , Dana Stein
    • , Inbar Cohen-Gihon
    • , Shlomi Lazar
    • , Hila Gutman
    • , Itai Glinert
    • , Lilach Cherry
    • , Yaron Vagima
    • , Shirley Lazar
    • , Shay Weiss
    • , Amir Ben-Shmuel
    • , Roy Avraham
    • , Reut Puni
    • , Edith Lupu
    • , Elad Bar-David
    • , Assa Sittner
    • , Noam Erez
    • , Ran Zichel
    • , Emanuelle Mamroud
    • , Ohad Mazor
    • , Haim Levy
    • , Orly Laskar
    • , Shmuel Yitzhaki
    • , Shmuel C. Shapira
    • , Anat Zvi
    • , Adi Beth-Din
    • , Nir Paran
    •  & Tomer Israely
  • Article
    | Open Access

    Blood eosinophil (EOS) counts may serve as risk factors for human coronary heart diseases. Here the authors show that increased circulating and myocardial EOS after myocardial infarction play a cardioprotective role by reducing cardiomyocyte death, cardiac fibroblast activation and fibrosis, and endothelium activation-mediated inflammatory cell accumulation.

    • Jing Liu
    • , Chongzhe Yang
    • , Tianxiao Liu
    • , Zhiyong Deng
    • , Wenqian Fang
    • , Xian Zhang
    • , Jie Li
    • , Qin Huang
    • , Conglin Liu
    • , Yunzhe Wang
    • , Dafeng Yang
    • , Galina K. Sukhova
    • , Jes S. Lindholt
    • , Axel Diederichsen
    • , Lars M. Rasmussen
    • , Dazhu Li
    • , Gail Newton
    • , Francis W. Luscinskas
    • , Lijun Liu
    • , Peter Libby
    • , Jing Wang
    • , Junli Guo
    •  & Guo-Ping Shi
  • Article
    | Open Access

    Semiconductor spin-qubits with CMOS compatible architectures could benefit from the industrial capacity of the semiconductor industry. Here, the authors make the first steps in demonstrating this by showing single electron operations within a two-dimensional array of foundry-fabricated quantum dots.

    • Fabio Ansaloni
    • , Anasua Chatterjee
    • , Heorhii Bohuslavskyi
    • , Benoit Bertrand
    • , Louis Hutin
    • , Maud Vinet
    •  & Ferdinand Kuemmeth
  • Article
    | Open Access

    SthK, a cyclic nucleotide-gated ion channel from Spirochaeta thermophila activates slowly upon cAMP increase. Here, authors investigate cAMP-induced activation in purified SthK channels using stopped-flow assays and enzymatic catalysis and reveal that the cis/trans conformation of a conserved proline in the cyclic nucleotide-binding domain determines the activation kinetics of SthK.

    • Philipp A. M. Schmidpeter
    • , Jan Rheinberger
    •  & Crina M. Nimigean
  • Article
    | Open Access

    Histones, proteins that bind DNA, are toxic for pathogens outside cells but can also cause multi-organ damage as seen in sepsis. Here the authors develop small negatively charged molecules that can be used as histone antidotes, and show that they improve the phenotype in mouse models with histone-related pathologies.

    • Connor H. O’ Meara
    • , Lucy A. Coupland
    • , Farzaneh Kordbacheh
    • , Benjamin J. C. Quah
    • , Chih-Wei Chang
    • , David A. Simon Davis
    • , Anna Bezos
    • , Anna M. Browne
    • , Craig Freeman
    • , Dillon J. Hammill
    • , Pradeep Chopra
    • , Gergely Pipa
    • , Paul D. Madge
    • , Esther Gallant
    • , Courtney Segovis
    • , Angela F. Dulhunty
    • , Leonard F. Arnolda
    • , Imogen Mitchell
    • , Levon M. Khachigian
    • , Ross W. Stephens
    • , Mark von Itzstein
    •  & Christopher R. Parish