Articles

The develop of organic functional materials requires the exploration of the pressure dependent emissive mechanisms of molecular structures, conformations and stacking modes. Here, Tong et al propose a strategy for monitoring the pressure-induced fluorescence under multiple excitation channels.

Using one carbon compounds as feedstock is a promising approach in abating climate change. Here, the authors report the conversion of E. coli into a synthetic methylotroph that assimilates methanol via the ribulose monophosphate cycle and a set of distinctive mutations.

Efficient mobilization and expression of biosynthetic gene clusters (BGCs) into heterologous hosts is needed for microbial natural products discovery. Here, the authors improve the CONKAT-seq strategy by simultaneously capturing the BCGs into a single large insert library and demonstrate its ability to discover natural products with new structures and potent antibacterial activity.

Elastic anisotropy of liquid crystals elastomers is typically measured at low frequencies for the applications such as soft robotics, actuators, and origami. Here the authors study the elastic anisotropy of LCE using Brillouin light spectroscopy at gigahertz frequencies such as radio frequencies or 5 G cellular networks.

Here, Nishijima et al. perform a large-scale analysis of the human gut virome in the Japanese 4D cohort of 4198 deeply phenotyped individuals, revealing thousands of bacteriophage genomes, virus-bacteria interactions, and describing associations with various host and environmental factors.

The authors introduce differentially enhanced compressed ultrafast photography, a phase imaging platform that combines high speed and sensitivity. They visualise propagation of passive current flows along myelinated axons, and electromagnetic pulses in lithium niobate.

Despite archaeobotanical evidence for domesticated cereals, organic residue evidence is scarce. Here, the authors identify cereal-specific markers in pottery from Scottish ‘crannogs’, revealing the presence of cereals in Neolithic pottery which might have been mixed with dairy products as a milk-based gruel.

Van der Waals crystals allow for magnetism down to the monolayer limit, however, this magnetism, and frequently the material itself, is fragile. Ferrites, conversely, have robust material stability and magnetic order, but are three dimensional. Here the authors succeed in creating a single unit cell thickness of Cobalt Ferrite via chemical vapour deposition, with hard magnetic properties, and curie temperature exceeding room temperature.

The singlet fission mechanism is still not relatively well understood, except for polyacenes. Here, the authors demonstrate that in diketopyrrolopyrrole supramolecular assemblies, both singlet fission and intersystem crossing can simultaneously happen.

Protein kinase-mediated phosphorylation plays a critical role in many biological processes. Here the authors develop a trans-omics-based algorithm called Central Kinase Inference to integrate quantitative transcriptomic and phosphoproteomic data, finding that PIM1 promotes hepatic conversion by suppressing reprogramming-induced ferroptosis and cell cycle arrest.

SIVmac239 infection of macaques is a favored model of human HIV infection, but antibody-mediated protection for SIVmac239 is insufficiently understood. Here, Zhao and Berndsen et al isolated nAbs and confirmed protection against SIVmac239 infection in passive transfer studies in macaques. The nAb was used to provide the first high-resolution structure of a rhesus SIV trimer by CryoEM. Analysis of the glycosylation pattern of this SIV trimer suggests a denser glycan shield on Env for rhesus SIV compared to chimpanzee SIV or HIV-1, which partially explains the poor nAb response of rhesus macaques to SIVmac239 infection.

Dissipatively coupled oscillators, describing lossy flows in power grids, are challenging to analyze due to asymmetry of couplings. Here, Delabays et al. reveal counterintuitive behaviours of increased capacity and increased stability in a network of lossy oscillators.

Lactic acid from glycolytic metabolism of cancer cells has been associated with immune suppressive functions. Here authors show that lactate, when depart from the acidic protons, inhibits histone deacetylases in CD8 + T cells, which turns them into potent anti-tumour immune cells.

Existing methods for generating sgRNA predictions do not account for the tracrRNA sequence. Here the authors report an on-target model, Rule Set 3, to generate optimal predictions for multiple tracrRNA variants, and validate this on a new dataset of sgRNAs showing improvement over prior prediction models.

Here, the authors use climate and resource availability, to statistically model the limiting factors in the dynamics of hunter-gatherer population densities in Europe between 21,000 and 8,000 years ago. They find that limiting factors varied spatiotemporally and the effects of these may be visible in the archaeological record.

Arising through multiple binding elements, multivalency can specify the avidity, duration, cooperativity, and selectivity of biomolecular interactions, but quantitative prediction and design of these properties has remained challenging. Here the authors enable facile analysis and engineering of multivalent binding by developing MVsim, a simulator that incorporates biochemical and biophysical parameters of interacting molecules and is accessible through a graphical user interface.

A challenge for synthetic biology is the design and construction of prototissue. Here, the authors spatially segregate layers of enzyme-decorated coacervate protocells as a model prototissue capable of chemical signal processing and modulating outputs of nitric oxide to inhibit blood clot formation.

Many archived tumour samples are stored as formalin-fixed and paraffin-embedded (FFPE) blocks, but this treatment can impact downstream genomics analyses. Here, the authors derive the mutational signatures of formalin on the cancer genome, and present FFPEsig, an algorithm that can distinguish and correct FFPE mutational signatures in archived cancer samples.

This study presents results from a SARS-CoV-2 genomic surveillance study at a university campus in which ~2,000 samples were sequenced over five months. The authors document the replacement of Delta with Omicron as the dominant variant, and describe clinical characteristics and transmission dynamics.

mRNA vaccines have been shown to prevent SARS-CoV-2 infection and reduce hospitalization and mortality rates. Here, the authors show evidence of long-term memory CD8 + T cells in individuals who received the BNT162b2 SARS-CoV-2 mRNA vaccine.