Volume 17 Issue 4, April 2015

Volume 17 Issue 4

Human fibroblasts plated on circular fibronectin islands show self-organization of the actin cytoskeleton into a chiral pattern.p445

Review

  • Review Article |

    Burroughs and DeBerardinis discuss the metabolic pathways that tumour cells employ to promote their survival and proliferation, and present the current approaches to study cancer metabolism in an in vivo setting.

    • Lindsey K. Boroughs
    •  & Ralph J. DeBerardinis

News and Views

  • News & Views |

    Excess caloric intake leads to both the growth of existing fat cells and the generation of new adipocytes. New findings show that PI3K–Akt2 signalling is involved in the differentiation of adult adipose precursor cells — a pathway not required for adipogenesis in the embryo.

    • Evan D. Rosen
  • News & Views |

    The kinase AMPK, a sensor of cellular energy stress, has been shown to oppose the growth-promoting activity of YAP, the transcriptional co-activator downstream of the Hippo signalling pathway. This finding may help to explain why the antidiabetic drug metformin, for which AMPK is a key effector, is linked to cancer-protective activity.

    • Iswar K. Hariharan

Articles

  • Article |

    Visvader and colleagues report that in the mouse mammary gland, EGF and mTOR signalling induce expression of the anti-apoptotic Bcl2 family member Mcl-1 and show that this is required for the survival of milk-producing mammary epithelial cells.

    • Nai Yang Fu
    • , Anne C. Rios
    • , Bhupinder Pal
    • , Rina Soetanto
    • , Aaron T. L. Lun
    • , Kevin Liu
    • , Tamara Beck
    • , Sarah A. Best
    • , François Vaillant
    • , Philippe Bouillet
    • , Andreas Strasser
    • , Thomas Preiss
    • , Gordon K. Smyth
    • , Geoffrey J. Lindeman
    •  & Jane E. Visvader
  • Article |

    Rodeheffer and colleagues show that a high-fat diet in mice activates Akt2 signalling in adipocyte precursors within white adipose tissue deposits, leading to their proliferation and differentiation into adipocytes, and to obesity.

    • Elise Jeffery
    • , Christopher D. Church
    • , Brandon Holtrup
    • , Laura Colman
    •  & Matthew S. Rodeheffer
  • Article |

    Trepat and colleagues conduct a systematic analysis of how key cell–cell adhesion molecules affect intercellular forces and epithelial monolayer dynamics.

    • Elsa Bazellières
    • , Vito Conte
    • , Alberto Elosegui-Artola
    • , Xavier Serra-Picamal
    • , María Bintanel-Morcillo
    • , Pere Roca-Cusachs
    • , José J. Muñoz
    • , Marta Sales-Pardo
    • , Roger Guimerà
    •  & Xavier Trepat
  • Article |

    Han and colleagues reveal that the phosphatase Ppm1b dephosphorylates the kinase Rip3 to negatively regulate necroptosis in vitro and in vivo.

    • Wanze Chen
    • , Jianfeng Wu
    • , Lisheng Li
    • , Zhengmao Zhang
    • , Junming Ren
    • , Yaoji Liang
    • , Fenfang Chen
    • , Chao Yang
    • , Zhenru Zhou
    • , Sheng Sean Su
    • , Xinru Zheng
    • , Zhirong Zhang
    • , Chuan-Qi Zhong
    • , Haoqiang Wan
    • , Mu Xiao
    • , Xia Lin
    • , Xin-Hua Feng
    •  & Jiahuai Han
  • Article |

    Bershadsky and colleagues show that cells confined to circular adhesive patterns exhibit defined and dynamic self-assembly of their actin cytoskeleton into a chiral pattern with defined handedness, potentially informing left–right cell asymmetry.

    • Yee Han Tee
    • , Tom Shemesh
    • , Visalatchi Thiagarajan
    • , Rizal Fajar Hariadi
    • , Karen L. Anderson
    • , Christopher Page
    • , Niels Volkmann
    • , Dorit Hanein
    • , Sivaraj Sivaramakrishnan
    • , Michael M. Kozlov
    •  & Alexander D. Bershadsky
  • Article |

    Studying spindle formation in fission yeast, Cooper and colleagues discover a role for the meiotic telomere bouquet in supporting centromere assembly.

    • Michael Klutstein
    • , Alex Fennell
    • , Alfonso Fernández-Álvarez
    •  & Julia Promisel Cooper
  • Article |

    By live imaging of mouse oocytes, Verlhac and colleagues demonstrate that actin-coated vesicles together with myosin Vb participate in centring of the nucleus by creating a gradient of cytoplasmic forces.

    • Maria Almonacid
    • , Wylie W. Ahmed
    • , Matthias Bussonnier
    • , Philippe Mailly
    • , Timo Betz
    • , Raphaël Voituriez
    • , Nir S. Gov
    •  & Marie-Hélène Verlhac
  • Article |

    In two related papers, Chen and colleagues and Guan and colleagues report a crucial role for the AMPK and Hippo pathways in glucose homeostasis. Starvation triggers AMPK-mediated phosphorylation and inactivation of YAP.

    • Wenqi Wang
    • , Zhen-Dong Xiao
    • , Xu Li
    • , Kathryn E. Aziz
    • , Boyi Gan
    • , Randy L. Johnson
    •  & Junjie Chen
  • Article |

    In two related papers, Chen and colleagues and Guan and colleagues report a crucial role for the AMPK and Hippo pathways in glucose homeostasis. Starvation triggers AMPK-mediated phosphorylation and inactivation of YAP.

    • Jung-Soon Mo
    • , Zhipeng Meng
    • , Young Chul Kim
    • , Hyun Woo Park
    • , Carsten Gram Hansen
    • , Soohyun Kim
    • , Dae-Sik Lim
    •  & Kun-Liang Guan
  • Article |

    Sabapathy and colleagues report that under hypoxic conditions, HIF-1α-mediated repression of the Siah1 ubiquitin ligases leads to stabilization of TAp73, which in turn promotes tumour angiogenesis.

    • Iqbal Dulloo
    • , Beng Hooi Phang
    • , Rashidah Othman
    • , Soo Yong Tan
    • , Aadhitthya Vijayaraghavan
    • , Liang Kee Goh
    • , Marta Martin-Lopez
    • , Margarita M. Marques
    • , Chun Wei Li
    • , De Yun Wang
    • , Maria Carmen Marín
    • , Wa Xian
    • , Frank McKeon
    •  & Kanaga Sabapathy

Letter

  • Letter |

    Bergert et al. use theoretical modelling and cell-based experiments to show that adhesion-independent cell migration is powered by nonspecific substrate friction, with smaller forces exerted compared with those of focal-adhesion-dependent movement.

    • Martin Bergert
    • , Anna Erzberger
    • , Ravi A. Desai
    • , Irene M. Aspalter
    • , Andrew C. Oates
    • , Guillaume Charras
    • , Guillaume Salbreux
    •  & Ewa K. Paluch

Retraction

Errata

Corrigenda