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Polarity cues regulate intestinal stem cell fate. Böttcher et al. demonstrate that mouse intestinal stem cells, which express the Wnt/planar cell polarity reporter Flattop, are primed either towards the enteroendocrine or Paneth cell lineage.
Gao et al. developed a CRISPR–Cas9-based system in which sgRNA production is controlled by the endogenous promoter to monitor the expression of weakly expressed genes and long non-coding RNAs in mammalian cells.