Abstract
α-AMYLASE is synthesised de novo in response to the plant hormone gibberellic acid (GA3) in the aleurone layers of barley grains1. This enzyme induction depends on RNA and protein synthesis, as determined by the use of inhibitors2–4, and it occurs after a temperature-dependent lag period5. The biochemical events of the lag period have been studied with respect to ionic requirements5–7, early protein synthesis8, RNA synthesis9–12, polysome formation13 and lipid synthesis and turnover14–17. It has been postulated that new mRNA for α-amylase synthesis might be produced in response to the hormone2,3 and there is evidence that GA3 enhances the incorporation of labelled ribonucleosides into poly(A)-containing RNA of barley aleurone layers18–20. Stimulation could be detected about 4 h after hormone treatment and although GA3 probably stimulates synthesis of a limited number of proteins, there was stimulation of a wide size range of poly(A)-containing RNAs. Thus it was not possible to say that GA3 had a selective effect on the synthesis of the mRNA for α-amylase.
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HIGGINS, T., ZWAR, J. & JACOBSEN, J. Gibberellic acid enhances the level of translatable mRNA for α-amylase in barley aleurone layers. Nature 260, 166–169 (1976). https://doi.org/10.1038/260166a0
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DOI: https://doi.org/10.1038/260166a0
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