Volume 50 Issue 1, January 2021

New wrinkles on the Nobel prize-winning method are creating new efficiencies, and new research opportunities.

In this Perspective, the authors highlight the importance of understanding the background genetics of the zebrafish, Danio rerio, and suggest ways that the field might better report genetic information and maintain breeding colonies to improve the transparency and reproducibility of zebrafish research.

Poon, Y-Y et al. re-visit the effects of isoflurane on cardiovascular functions by measuring temporal changes in blood pressure, heart rate, cardiac performance, baroreflex-mediated sympathetic vasomotor tone, cardiac vagal baroreflex, functional connectivity within baroreflex neural circuits, carotid or cerebral blood flow, respiratory rate and blood gas in mice exposed to 1.5% (vol/vol) isoflurane.