Polypropylene-based products are commonly used for food preparation and storage, but their capacity to release microplastics is poorly understood. We investigated the potential exposure of infants to microplastics from consuming formula prepared in polypropylene (PP) infant feeding bottles (IFBs). Here, we show that PP IFBs release microplastics with values as high as 16,200,000 particles per litre. Scenario studies showed that PP IFB sterilization and exposure to high-temperature water significantly increase microplastic release. A 21-d test of PP IFBs showed periodic fluctuations in microplastic release. To estimate the potential global exposure to infants up to 12 months old, we surveyed 48 regions, finding values ranging from 14,600–4,550,000 particles per capita per day, depending on the region. We demonstrate that infant exposure to microplastics is higher than was previously recognized due to the prevalence of PP-based products used in formula preparation and highlight an urgent need to assess whether exposure to microplastics at these levels poses a risk to infant health.
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All data analysed in this study are contained within the Supplementary Information. The raw data that support the findings of this study are available from the corresponding author upon request to J.J.W. or from the Figshare repository at https://figshare.com/account/home#/projects/88406. Source data are provided with this paper.
The mathematical algorithm used for MP exposure assessment has been included in the Methods. The sales data mining software Jungle Scout is available at https://www.junglescout.com/.
Thompson, R. C. et al. Lost at sea: where is all the plastic? Science 304, 838 (2004).
Efferth, T. & Paul, N. W. Threats to human health by great ocean garbage patches. Lancet Planet. Health 1, e301–e303 (2017).
Gupta, J. et al. Communicating the health of the planet and its links to human health. Lancet Planet. Health 3, e204–e206 (2019).
De Sá, L. C., Oliveira, M., Ribeiro, F., Rocha, T. L. & Futter, M. N. Studies of the effects of microplastics on aquatic organisms: what do we know and where should we focus our efforts in the future? Sci. Total Environ. 645, 1029–1039 (2018).
Wright, S. L. & Kelly, F. J. Plastic and human health: a micro issue? Environ. Sci. Technol. 51, 6634–6647 (2017).
Meissner, R. Ocean governance for human health and the role of the social sciences. Lancet Planet. Health 2, e275–e276 (2018).
The Lancet Planetary Health Microplastics and human health—an urgent problem. Lancet Planet. Health 1, e254 (2017).
Smith, M., Love, D. C., Rochman, C. M. & Neff, R. A. Microplastics in seafood and the implications for human health. Curr. Environ. Health Rep. 5, 375–386 (2018).
Lehner, R., Weder, C., Petri-Fink, A. & Rothen-Rutishauser, B. Emergence of nanoplastic in the environment and possible impact on human health. Environ. Sci. Technol. 53, 1748–1765 (2019).
Cox, K. D. et al. Human consumption of microplastics. Environ. Sci. Technol. 53, 7068–7074 (2019).
Schwabl, P. et al. Detection of various microplastics in human stool: a prospective case series. Ann. Intern. Med. 171, 453–457 (2019).
Jin, Y., Lu, L., Tu, W., Luo, T. & Fu, Z. Impacts of polystyrene microplastic on the gut barrier, microbiota and metabolism of mice. Sci. Total Environ. 649, 308–317 (2019).
Lu, L., Wan, Z., Luo, T., Fu, Z. & Jin, Y. Polystyrene microplastics induce gut microbiota dysbiosis and hepatic lipid metabolism disorder in mice. Sci. Total Environ. 631, 449–458 (2018).
Mattsson, K. et al. Brain damage and behavioural disorders in fish induced by plastic nanoparticles delivered through the food chain. Sci. Rep. 7, 11452 (2017).
Microplastics in Drinking-Water (World Health Organization, 2019).
Oßmann, B. E. et al. Small-sized microplastics and pigmented particles in bottled mineral water. Water Res. 141, 307–316 (2018).
Schymanski, D., Goldbeck, C., Humpf, H.-U. & Fürst, P. Analysis of microplastics in water by micro-Raman spectroscopy: release of plastic particles from different packaging into mineral water. Water Res. 129, 154–162 (2018).
Hernandez, L. M. et al. Plastic teabags release billions of microparticles and nanoparticles into tea. Environ. Sci. Technol. 53, 12300–12310 (2019).
Geyer, R., Jambeck, J. R. & Law, K. L. Production, use, and fate of all plastics ever made. Sci. Adv. 3, e1700782 (2017).
Tripathi, D. Practical Guide to Polypropylene (Rapra Technology, 2002).
Zimmermann, L., Dierkes, G., Ternes, T. A., Völker, C. & Wagner, M. Benchmarking the in vitro toxicity and chemical composition of plastic consumer products. Environ. Sci. Technol. 53, 11467–11477 (2019).
Zhao, S. et al. Analysis of suspended microplastics in the Changjiang Estuary: implications for riverine plastic load to the ocean. Water Res. 161, 560–569 (2019).
Pan, Z., Liu, Q., Sun, Y., Sun, X. & Lin, H. Environmental implications of microplastic pollution in the Northwestern Pacific Ocean. Mar. Pollut. Bull. 146, 215–224 (2019).
Efimova, I., Bagaeva, M., Bagaev, A., Kileso, A. & Chubarenko, I. P. Secondary microplastics generation in the sea swash zone with coarse bottom sediments: laboratory experiments. Front. Mar. Sci. 5, 313 (2018).
Klein, S., Dimzon, I. K., Eubeler, J. & Knepper, T. P. in Freshwater Microplastics—Emerging Environmental Contaminants? 51–67 (Springer, 2018).
How to Prepare Formula for Bottle-Feeding at Home (World Health Organization, 2007).
Zhao, S., Danley, M., Ward, J. E., Li, D. & Mincer, T. J. An approach for extraction, characterization and quantitation of microplastic in natural marine snow using Raman microscopy. Anal. Methods 9, 1470–1478 (2017).
Simpson, R. J. & Selke, S. E. in Emerging Technologies in Plastics Recycling (ed. Andrews, G. D.) Ch. 18, 232–240 (ACS Publications, 1992).
Longo, C., Savaris, M., Zeni, M., Brandalise, R. N. & Grisa, A. M. C. Degradation study of polypropylene (PP) and bioriented polypropylene (BOPP) in the environment. Mater. Res. 14, 442–448 (2011).
Victora, C. G. et al. Breastfeeding in the 21st century: epidemiology, mechanisms, and lifelong effect. Lancet 387, 475–490 (2016).
Neves, P. A. et al. Infant formula consumption is positively correlated with wealth, within and between countries: a multi-country study. J. Nutr. 150, 910–917 (2020).
Gallego-Schmid, A., Jeswani, H. K., Mendoza, J. M. F. & Azapagic, A. Life cycle environmental evaluation of kettles: recommendations for the development of eco-design regulations in the European Union. Sci. Total Environ. 625, 135–146 (2018).
Sturm, M. T., Kluczka, S., Wilde, A. & Schuhen, K. Determination of particles produced during boiling in differenz plastic and glass kettles via comparative dynamic image analysis using FlowCam®. Analytik NEWS (14 February 2019).
Safe Preparation, Storage and Handling of Powdered Infant Formula: Guidelines (World Health Organization, 2007).
Guidance for Health Professionals on Safe Preparation, Storage and Handling of Powdered Infant Formula (Food Standards Agency & Department of Health, 2005).
Angulo, F. J., Cahill, S. M., Wachsmuth, I. K., de Lourdes Costarrica, M. & Embarek, P. K. B. Powdered infant formula as a source of Salmonella infection in infants. Clin. Infect. Dis. 46, 268–273 (2008).
National Health Service, UK. Infant Feeding Survey—UK, 2010 (NHS Digital, 2012).
Breastfeeding and Infant Feeding Practices, Infant Feeding Practices Study II (Centers for Disease Control and Prevention, 2014).
Morishita, Y. et al. Distribution of silver nanoparticles to breast milk and their biological effects on breast-fed offspring mice. ACS Nano 10, 8180–8191 (2016).
Melnik, E. et al. Transfer of silver nanoparticles through the placenta and breast milk during in vivo experiments on rats. Acta Naturae 5, 107–115 (2013).
Gao, X. et al. Effects of developmental exposure to TiO2 nanoparticles on synaptic plasticity in hippocampal dentate gyrus area: an in vivo study in anesthetized rats. Biol. Trace Elem. Res. 143, 1616–1628 (2011).
Zhang, C. et al. Induction of size-dependent breakdown of blood–milk barrier in lactating mice by TiO2 nanoparticles. PLoS ONE 10, e0122591 (2015).
Cai, J., Zang, X., Wu, Z., Liu, J. & Wang, D. Translocation of transition metal oxide nanoparticles to breast milk and offspring: the necessity of bridging mother–offspring-integration toxicological assessments. Environ. Int. 133, 105153 (2019).
Oßmann, B. E. et al. Development of an optimal filter substrate for the identification of small microplastic particles in food by micro-Raman spectroscopy. Anal. Bioanal. Chem. 409, 4099–4109 (2017).
This work was supported by Enterprise Ireland (grant number CF20180870), Science Foundation Ireland (grants numbers 12/RC/2278, 16/IA/4462 and 16/RC/3889), the School of Engineering Scholarship at Trinity College Dublin, and the China Scholarship Council (201506210089 and 201608300005). We also thank Keyence for help. The presentation of the material in this publication does not imply the expression of any opinion whatsoever on the part of Trinity College Dublin about specific companies or of certain manufacturers’ products and does not imply that they are endorsed, recommended, criticised or otherwise by Trinity College Dublin in preference to others of a similar nature. Errors and omissions excepted. All reasonable precautions have been taken to verify the information contained in this publication. However, the published material is being distributed without warranty of any kind, either expressed or implied. The responsibility for the interpretation and use of the material lies with the reader. In no event shall Trinity College Dublin be liable for damages arising from its use.
The authors declare no competing interests.
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Li, D., Shi, Y., Yang, L. et al. Microplastic release from the degradation of polypropylene feeding bottles during infant formula preparation. Nat Food 1, 746–754 (2020). https://doi.org/10.1038/s43016-020-00171-y
Nature Food (2020)
International Journal of Environmental Research and Public Health (2020)