Abstract
The thalamic reticular nucleus (TRN) is a brain region that influences vital neurobehavioral processes, including executive functioning and the generation of sleep rhythms. TRN dysfunction underlies hyperactivity, attention deficits, and sleep disturbances observed across various neurodevelopmental disorders. A specialized sarco-endoplasmic reticulum calcium (Ca2+) ATPase 2 (SERCA2)-dependent Ca2+ signaling network operates in the dendrites of TRN neurons to regulate their bursting activity. Phospholamban (PLN) is a prominent regulator of SERCA2 with an established role in myocardial Ca2+-cycling. Our findings suggest that the role of PLN extends beyond the cardiovascular system to impact brain function. Specifically, we found PLN to be expressed in TRN neurons of the adult mouse brain, and utilized global constitutive and innovative conditional genetic knockout mouse models in concert with electroencephalography (EEG)-based somnography and the 5-choice serial reaction time task (5-CSRTT) to investigate the role of PLN in sleep and executive functioning, two complex behaviors that map onto thalamic reticular circuits. The results of the present study indicate that perturbed PLN function in the TRN results in aberrant TRN-dependent phenotypes in mice (i.e., hyperactivity, impulsivity and sleep deficits) and support a novel role for PLN as a critical regulator of SERCA2 in the TRN neurocircuitry.
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Introduction
The thalamic reticular nucleus (TRN) is a critical but poorly understood structure in the mammalian forebrain involved in regulating complex behaviors, including sleep and executive functioning1. The TRN is uniquely situated between the cortex and the thalamus, and consists of a thin layer of gamma-aminobutyric acid (GABA)-ergic neurons that inhibit thalamic relay cells2. Interestingly, the activity of TRN neurons relies heavily on calcium (Ca2+) regulation. Depending on their resting membrane potential, TRN neurons fire in two distinct modes; at depolarized states, they generate sodium (Na+) discharges, but when hyperpolarized they give rise to repetitive “low-threshold” Ca2+ transients crowned by high-frequency Na+ bursts3,4,5. Notably, a highly specialized sarco-(SR)-endoplasmic reticulum (ER) Ca2+ ATPase2 (SERCA2)-dependent Ca2+ signaling network operates in the dendrites of TRN neurons to generate and regulate the strength of their high-frequency bursting activity3,6.
In mammals, three differentially expressed SERCA genes (known as ATP2A1-3 in humans) encode at least ten SERCA isoforms7. Of all SERCA proteins, SERCA2 is the most widely distributed, with the SERCA2b isoform being ubiquitously expressed in nerve cells8. Given its critical role in ensuring appropriate ER Ca2+ store filling9,10, perturbed function of the SERCA2 may result in aberrations in intracellular Ca2+-signaling cascades that may in turn impact neuronal function11. Indeed, SERCA2-dependent dysregulation of neuronal Ca2+ homeostasis has been implicated in the pathophysiology of devastating disorders that affect cognition, including schizophrenia, Alzheimer’s disease, and Darier’s disease12,13,14,15,16,17. Of note, Phospholamban (PLN) is a critical regulator of the SERCA2 with an established role in maintaining Ca2+ homeostasis in cardiac, skeletal and smooth muscle cells18,19,20. Specifically, in myocardial cells dephosphorylated PLN binds to SERCA2 and reduces its affinity for Ca2+, thus decreasing Ca2+ uptake into the SR18. The role of PLN in SR Ca2+ cycling and cardiomyocyte contractility is well defined; indeed, increased PLN inhibition of SR Ca2+ cycling is a major characteristic of human and experimental heart failure18,21,22,23,24. PLN is thought to serve as an inhibitor of SERCA2 in the heart and phosphorylation of PLN by b-adrenergic stimulation relieves this inhibitory action18. Indeed, genetic ablation of Pln in mice (i.e., Pln−/−) results in phenotypically normal animals with hyperdynamic hearts, as assessed in isolated cardiomyocytes and in vivo21. Minor compensatory mechanisms include downregulation of the ryanodine receptor (RyR) to compensate for the increased Ca2+ content in the SR and metabolic adaptations to establish a new energetic steady state to meet in the hyperdynamic PLN-deficient hearts25. Overall, Pln−/− mice do not show gross developmental abnormalities or significant reduction in lifespan, they have normal body weight and heart rate, and systolic blood pressure similar to their wild type littermates, as assessed by tail-cuff measurements in unanesthetized mice25. In isolated work-performing heart preparations, Pln deletion was found to enhance several cardiovascular and contractile parameters including cardiac contractility, intraventricular pressure, and aortic blood pressure25. In this context, Pln−/− mice have historically served as an invaluable model system for elucidating the Ca2+-handling role of PLN in myocardial cells and the cardiovascular system21,26,27,28.
Even though SERCA2 is ubiquitously expressed in neurons8,29,30, a number of early Northern blotting studies had failed to detect Pln mRNA expression in the brain of humans or mice31,32. Excitingly, we found that the Pln gene is selectively expressed at the protein level in the mouse GABAergic TRN neurons, and thus hypothesized that PLN may be necessary for modulating critical behaviors that map onto thalamic reticular circuits. In order to address this hypothesis, we employed a combination of existing global (i.e., Pln−/−) and novel conditional genetic knockout mouse models. Given the TRN-specific expression profile of PLN, we developed a novel GABAergic Cre-conditional Pln knockout mouse line (cKO) in which PLN expression is selectively ablated in the GABAergic TRN neurons, and further assessed how PLN deficiency in the TRN impacts locomotor activity, executive functioning and sleep architecture, three vital behaviors that map onto thalamic reticular circuits. Overall, our findings provide the first evidence that the PLN/SERCA2-mediated Ca2+ signaling is critical for information gating by the TRN and is required for appropriate elaboration of sleep, cognitive and locomotor behaviors.
Materials and methods
Animals
Animals were bred in the animal facility of the University of Dayton (Dayton, OH) under standard 12 h light/dark schedule and housed in groups of 3–4 of the same genetic background and sex after weaning, unless otherwise stated, in standard transparent mouse cages (300 mm × 160 mm × 110 mm; Alternative Design, AR, USA). Adult (i.e., 8–12 month old) male and female Pln−/− and wild type Pln+/+ 129SvJ littermate mice derived from breeders provided by Dr. Evangelia G. Kranias (University of Cincinnati, Cincinnati, OH, USA; Biomedical Research Foundation of the Academy of Athens, Athens, Greece)33,26. The generation of the new floxed Pln mouse line is described below. Mice were housed under standard conditions (i.e., 22 °C ± 2 °C; 30–60% humidity; 12-h light/dark cycle, lights on at 7:00 AM), and given access to food and water ad libitum, unless otherwise stated. Mouse genotypes were independently determined in littermates derived from heterozygote crosses using standard genotyping procedures. All animals were habituated to the experimental conditions for at least one week prior to any experiments, while behavioral testing occurred during the light cycle, unless otherwise stated. All experiments were conducted in accordance with the National Institute of Health (NIH) Guide for the Care and Use of Laboratory Animals (NIH Publications No. 80-23; revised 1978) and were approved by the University of Dayton Animal Care and Use Committee (IACUC). Methods of euthanasia were consistent with the American Veterinary Medical Association (AVMA) Guidelines for the Euthanasia of Animals, and included carbon dioxide (CO2) inhalation, cardiac perfusion in anesthetized animals using 4% paraformaldehyde (PFA) fixative solution, cervical dislocation, or pentobarbital overdose (150 mg/kg; ip) followed by decapitation in accordance with IACUC-approved protocols. All experiments in this study are reported according to ARRIVE guidelines.
Construction of the Pln LoxP-Cre conditional knockout mouse line (cKO)
The construction of the floxed Pln (i.e., Plnlox/lox) mouse line was funded by an inaugural STEM Catalyst grant from the University of Dayton to Dr. Pitychoutis and service was provided by the Transgenic Animal and Genome Editing Core at Cincinnati Children’s Hospital Medical Center (CCHMC; Cincinnati, OH) (Fig. 1). Specifically, the floxed Pln allele was generated by CRISPR/Cas9 using a dual-sgRNA strategy to cut the genomic DNA fragment containing the entire coding sequence and repair it with a sgRNA-resistant long ssDNA donor with two loxP sites, the left homologous arm at 76nt and the right at 109nt, the so-called Easi-CRISPR method34 (Fig. 1a). The sgRNA target sequences were selected according to the on- and off-target scores from the web tool CRISPOR (http://crispor.tefor.net)35. sgRNAs were transcribed in vitro using the MEGAshorscript T7 kit (ThermoFisher) and purified by the MEGAclear Kit (ThermoFisher) and stored at − 80 °C. To validate their activity, we incubated sgRNAs and Cas9 protein (IDT) at 37 °C for 10 min to form ribonucleoproteins (RNP). The final concentrations were 50 ng/ul of each sgRNA and 150 ng/ul Cas9 protein. The RNP was delivered into a small batch of mouse zygotes by electroporation using a Genome Editor electroporator (BEX; 30 V, 1 ms width, and 5 pulses with 1 s interval). Zygotes were cultured for 4 days to blastocysts and genotyped individually, where we found that 50% embryos showed the large deletion between two cut sites. The long ssDNA donor was produced in-house by releasing it from a dsDNA plasmid by double nickase digestion (Nb.BsmI), followed by denaturing in the RNA-loading buffer at 70 °C for 5 min then on ice for 1 min, electrophoresis, and gel extraction with QIAquick Gel Extraction Kit (Qiagen). The mosaic floxed mice were created by electroporating zygotes on the C57BL/6 genetic background with the RNP and long ssDNA donor at the concentration of 50 ng/uL. Electroporated zygotes were transferred into the oviductal ampulla of pseudopregnant CD-1 females on the same day. Pups were born and genotyped by PCR and Sanger sequencing. One mouse (i.e., Mouse #5040) containing the LoxP sites was then selected as confirmed by primer pairs P1-P3 and P4-P6 for 5’ and 3’ end (Fig. 1b,c). The selected floxed Pln (Plnlox/lox) mosaic female mouse was backcrossed to a wild type male mouse; the resulting pups were genotyped by PCR and Sanger sequencing to confirm the presence of both 5’- and 3’-LoxP sites (Fig. 1d). Upon expansion of the colony, homozygote floxed Plnlox/lox mice were crossed with another transgenic mouse line expressing the Cre recombinase in GABAergic GAD65+ neurons, Gad2-Cre+/+;Pln+/+ (i.e., B6J.Cg-Gad2tm2(cre)Zjh/MwarJ mice; The Jackson Laboratory, stock No. 028867). Floxed PLN heterozygotes carrying the Cre recombinase were selected with PCR genotyping and crossed again with Plnlox/lox mice to generate litters containing the Gad2-Cre+/0;Plnlox/lox (cKO) and their Gad2-Cre0/0; Plnlox/lox (Control) littermates (Fig. 1e).
PLN mRNA and protein expression assessments
Total RNA was isolated from snap-frozen whole mouse brain with the Trizol protocol (Life Technologies) and cDNA was synthesized with the Superscript-RT kit (Invitrogen) using oligo-dT primers. PLN mRNA expression in the whole mouse brain was assessed using RT-PCR procedures and the following primers: Forward T1T2: CCCATAAACCTGGGAACAGA; Reverse T1T2: TAGCCGAGCGAGTGAGGTAT, yielding a transcript variant of 235 bp. PLN protein expression was detected in the mouse heart using standard immunoblotting procedures. Briefly, whole heart tissue was collected from Pln+/+, Pln−/−, Control and cKO mice and sonicated in RIPA buffer supplemented with protease and phosphatase inhibitors (150 nM NaCl, 1.0% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0). Total protein quantification was assessed via the BCA assay (Pierce BCA Protein Assay Kit #23227). For each sample, equal amounts of protein (i.e., 40ug) were loaded onto a 10% SDS-PAGE gel for electrophoresis and proteins were transferred onto a nitrocellulose membrane. Upon transferring, the blots were cut and probed simultaneously with primary antibodies for the detection of PLN (Invitrogen #MA3-922; 1:500) and GAPDH (Novus Biologicals #NB100-56875; 1:200 or Invitrogen #PA1987, 1:500) overnight (O/N) at 4 °C. Upon incubation with alkaline phosphatase-conjugated secondary antibodies (Invitrogen #A16014, #A16002; 1:5000), membranes were exposed to ECF substrate (GE Healthcare #1067873) for visualization in a UVP BioSpectrum 500 Imaging System.
Free-floating fluorescent immunohistochemistry (IHC) for detection of PLN immunoreactivity
Anesthetized mice were transcardially perfused with ice-cold 1xPBS followed by 4% paraformaldehyde (PFA) fixative solution. Isolated mouse brains were then post-fixed in 4%PFA O/N at 4 °C prior to sectioning using a Vibratome (Lafayette Instruments; IN, USA). 40 μm free-floating coronal Vibratome-cut sections were first incubated in a 10% normal goat serum (NGS) blocking solution in 1 × TBS-0.1% Triton X-100 for 1 h at room temperature (RT) and were then incubated O/N at 4 °C in primary antibody solution in 3% NGS. The next day sections were washed 3 × 15 min in 1xTBS, incubated in blocking solution for 1 h at RT, and were then incubated for 1 h at RT in the secondary antibody solution in 3% NGS in the dark. Following 3 × 15 min in 1xTBS washes, sections were mounted onto positively charged glass slides and coversliped using DAKO fluorescent mounting medium (Agilent Technologies S3023). A negative control (i.e., sections incubated in 3% NGS solution in the absence of primary antibody solution) was included in each IHC experiment. Images were taken with an Olympus Fluoview 3000 confocal microscope (PA, USA). Brain sections from the different mouse genotypes were processed concurrently on each IHC experiment, while imaging conditions (i.e., laser power, HV, gain and offset) were kept consistent throughout the samples within the same brain region and magnification of the respective experiment. Image processing (i.e., brightness and contrast), if needed, was kept to minimum and was equally applied to all images. The primary antibodies used in our single and/or double IHC experiments included mouse monoclonal PLN (Invitrogen #MA3-922; 1:100), rabbit polyclonal anti-GAD67 (Invitrogen #PA5-21397; 1:250), rabbit polyclonal anti-SERCA2 (Abcam #ab150435; 1:500), and rabbit polyclonal anti-GFAP (Abcam #ab7260, 1:500) and secondary antibodies goat anti-mouse Alexa Fluor 488 (Invitrogen #A32723TR; 1:500), goat anti mouse IgG2a Alexa Fluor 647 (Invitrogen #A21241, 1:500), and goat anti-rabbit Alexa Fluor 568 (Invitrogen #A21428; 1:500). IHC immunoreactivity for PLN, GAD-67 and SERCA2 was first assessed in single IHC experiments, and their co-localization in TRN neurons was further confirmed in double IHC experiments. The specificity of the primary PLN Ab used for IHC experiments was also validated using western blotting by the detection of PLN signal in the heart of Pln+/+ but not in Pln−/− mice (Fig. 1f).
Behavioral testing
Open field test (OFT)
Spontaneous locomotor activity and anxiety-like behavior were assessed by individually placing animals in the center of a novel OFT arena (45 cm × 45 cm × 40 cm). Both the horizontal distance traveled, and the time mice spent in the center of the arena were recorded and analyzed using the SMART v3.0 video tracking software (Panlab, Coulbourn Instruments, Harvard Apparatus, MA, US), as in previous studies from our lab36,37,38,39. Horizontal activity is reported as both total distance traveled during the entire session, as well as in separate 5-min bins that allow for the detailed investigation of the effects of PLN ablation on locomotor activity36,39,40.
Y-maze test
Spatial working memory was assessed using the Y-maze task, as previously described41,42. Briefly, mice were individually placed in the center of a Y-shaped fiber plastic light-colored opaque maze comprised of three identical arms (A, B and C), measuring 22 cm × 6.4 cm × 15 cm (120° from each other), and were allowed to freely explore all three arms over a 10-min period. A CCD camera fixed above the center of the maze allowed for digital video output and performance in the Y-maze task was blindly scored. Spontaneous alternation (per cent) was measured as an index of spatial working memory driven by the animal’s innate preference to explore novel areas43. Spontaneous alternations (SA) were defined as successful entries to three separate arms consecutively. Subsequently the SA% score was calculated using the formula below:
Object recognition and locational memory
Long-term object recognition memory (ORM) and object locational memory (OLM) were assessed using a two part protocol, as previously described44. The same mouse cohort first completed the OLM testing, followed by the ORM testing with 7 days (d) apart. Briefly, mice were subjected to 3 consecutive phases: (i) habituation (6d), (ii) training (1d), and (iii) testing (1d). All phases were conducted at dim light conditions (~ 47 Lux). During habituation, each mouse was introduced to an open non-translucent arena measuring 45 cm × 22.5 cm × 40 cm for 5 min per day and for 6 consecutive days. During the OLM training, two identical objects were placed in the arena in symmetrical positions. Each mouse was allowed to freely explore the arena for 10 min. The OLM test was conducted at 24 h following the training phase, and one object was moved to the opposite side of the arena. Mice were allowed to explore the arena for 5 min. At 7d following completion of OLM testing, mice were habituated to the circular ORM arena (24 cm in diameter). In the ORM training, two identical objects were placed into the arena in symmetrical locations and mice were allowed to explore the arena for 10 min. Two sets of objects were used in the ORM test to avoid innate preference for one object; familiar and novel objects were counterbalanced across groups. In the ORM testing, one object was replaced with a novel object of different shape and color, and mice were allowed to explore the arena for 5 min. All sessions were video-recorded and blindly scored for time spent interacting with each object.
Three-chamber social interaction test
Sociability and social memory were assessed as previously described45,46. Briefly, mice were individually introduced to a partitioned box and upon habituation underwent a two-session social test protocol. The first session assessed sociability, while the second session tested for preference for social novelty, that is indicative of social memory, as in our previous study46. The social test apparatus consisted of a transparent Plexiglass box with removable partitions, divided into three chambers; two equally sized distal chambers (40 cm × 20 cm, each) and one middle chamber (40 cm × 18 cm), with 5 cm openings between each chamber. Two cylindrical wire cages (10 cm in height, 9 cm bottom diameter) were used to contain the social stimuli. For the sociability test, the test animal was habituated to the middle chamber for 5 min. Subsequently, an unfamiliar wild-type mouse of the same sex (i.e., Stranger I) was positioned under one of the wire cages located in one of the distal chambers, while an empty cage was inserted in the other distal chamber. The dividers were then raised, and the test animal was allowed to freely explore all three chambers for 10 min. Following this session, a second unfamiliar mouse of the same sex (i.e., Stranger II) was inserted into the previously empty wire cage, and the test animal was left to continue exploring all chambers for 10 min. Time spent in close interaction with both cages was recorded. The release of the animals and the relative positioning sequence of the stranger mice were counterbalanced across different animals but were kept consistent for each individual test animal. All sessions were video-recorded and blindly scored for time spent interacting with each object.
EEG-based analysis of sleep architecture
Sleep architecture was assessed using electroencephalogram (EEG)/electromyogram (EMG)-based polysomnography, as previously described46,47,48. Briefly, anesthetized mice underwent aseptic stereotaxic surgery for the implantation of epidural EEG electrodes in the format of a commercially available prefabricated head mount (Pinnacle Technology, Lawrence, KS). The headmount consisted of a plastic 6-pin connector glued to a printed circuit board (PCB), where three EEG electrodes and two EMG electrodes were affixed. Four stainless-steel screws secured the head mount on the skull and served as the EEG electrodes. The two anterior screws (0.1 inches length) were placed 1.5 mm lateral to the midline and 1 mm anterior to the bregma, while the two parietal screws (0.125 inches length) were placed 1.5 mm lateral to the midline and approximately 2 mm anterior to lambda. Conductivity with the PCB was secured by silver epoxy application. In addition, two 1.5 cm long stainless-steel wires served as EMG electrodes and were embedded in the neck musculature. Upon fixation of the structure on the skull with dental cement, the neck incision was closed with sterile non-absorbing acrylic sutures and a topical analgesic was applied. Buprenorphine analgesia was provided, as needed. The mice were then left to recover and habituate in the circular recording cages for 7–10 days prior to being connected to the recording cable (i.e., pre-amplifier). Two days later, a polygraphic recording of the spontaneous sleep–wakefulness states was performed during 48 h, beginning at 7:00 pm. Subsequently, EEG/EMG recordings were blindly scored in 10 s epochs as wakefulness, non-rapid eye movement (NREM) sleep, or rapid eye movement (REM) sleep, following classical criteria49,50 using the Sirenia® analysis package (Pinnacle Technology, Inc. KS, USA). Spontaneous sleep–wakefulness patterns and the amounts of vigilance states were determined for each mouse throughout 48 h and summed over 24 h, as in our previous studies46. For bout analysis, a bout was defined as 3 or more consecutive epochs of one vigilance state. Total bout number and average bout length for wakefulness, NREM, and REM sleep were reported for the total recording period, dark period, and light period. Arousals during NREM sleep were defined as 1–2 epochs (i.e. below the bout threshold) of wakefulness during a period of NREM sleep. Sleep spindle analysis was not conducted as it is not a feature of the Sirenia® analysis package. Spectral analysis was conducted using the Fast Fourier Transform (Sirenia Sleep Pro, Pinnacle Technology Inc) to calculate the power (μV2) of delta (δ; 0.5–4 Hz), theta (θ; 4–8 Hz), alpha (α; 8–13 Hz), beta (β; 13–30 Hz), and gamma (γ; 30–40 Hz), frequency bands during each of the vigilance states. Total power of each sleep state was summed and normalized as a percentage of total power across all frequency bands.
5-Choice serial reaction time task (5-CSRTT) for the assessment of executive functioning
Impulsivity and attention were assessed using the 5-CSRTT in a 9-hole operant chamber using protocols provided by Lafayette Instrument Co (Lafayette Instrument Co., IN, USA) and based on previous studies from our lab and others51–53. Male and female control and cKO were housed throughout a reverse 24/h light/dark cycle, and all testing was performed during dark hours. Prior to training, mice were food restricted to 90% of free-feeding body weight. Following food restriction, mice were pre-exposed to the liquid reward (Yoohoo; Mott’s, Plano, TX, USA) at 24 h before magazine training to reduce neophobic reactions to the reward. The 5-CSRTT chamber features on one side a reward tray where liquid reward is dispensed through a pump, and on the other side 9 nose-poke holes with recessed lights. Nose-pokes were detected via infrared beam break and recorded automatically. Each hole and the reward tray have a light that illuminates conditionally. For this experiment, only nose-pokes in holes 1, 3, 5, 7, and 9 were recorded. Holes 2, 4, 6, and 8 remained inactive throughout all 5-CSRTT training and testing. Following pre-exposure to reward, mice began the magazine training schedule. During magazine training, the center nose-poke hole illuminated for 8 s, followed by dispensal of reward solution. Upon collecting the reward solution, a 5 s inter-trial interval (ITI) occurred prior to the next trial. Mice were required to complete 30 trials within the 30 min session to progress to the next phase. After magazine training, all mice were moved into a fixed ratio schedule (FR1). In this schedule, the center nose-poke hole remained lit until a nose-poke response was made, at which point it terminated and reward was dispensed. A magazine entry was required for the next trial to start. Mice completed a single session (30 min/50 trials, whichever was reached first) each day for 17 days (d). Criterion was 50 completed trials; all mice that reached criterion within 17d of FR1 training passed onto a progressive ratio (PR) session. Following the FR1, all mice completed a single PR session. This session was identical to the FR1, except that the number of nose-pokes required to dispense reward increased every third trial (i.e., 1,1,1,2,2,2,4,4,4,7,7,7…). The PR session was conducted for 60 min, or until no response was made for 5 min, defined as the breakpoint. After completion of the PR session, mice progressed to the basic 5-CSRTT testing paradigm. In this paradigm, a session begins with a dispensal of reward. After entry into the reward magazine, a 5 s ITI is initiated, during which all lights are off and the animal is required to inhibit its response. After the ITI, one of the five odd-numbered holes illuminated for a duration that remained constant for that session, followed by a 3 s limited hold where the light turned off but responses were still accepted. A nose-poke to the illuminated hole was recorded as a correct response, whereas a nose-poke to another hole was recorded as an incorrect response. If no response was made to any hole during the stimulus duration or the 3 s limited hold, an omission was recorded. Finally, nose-pokes during the ITI were recorded as premature responses. Reward was dispensed only after correct responses were made. All animals completed a single 30 min session each day, and performance measures were reported for the first day criteria was met (> 20 responses, with 50% response accuracy). Basic 5-CSRTT experiments were completed in 3 sub-phases with progressively decreasing stimulus duration (32 s, 16 s, 8 s). In order to progress in these sub-phases, mice needed to reach criteria for two consecutive sessions. Mice progressed at their own pace and were subjected to a single titration 5-CSRTT session following completion of basic 5-CSRTT testing. In the titration session, mice were subjected to a more difficult version of the basic 5-CSRTT. In this session, stimulus duration was titrated based upon an animal’s performance within that session. The stimulus duration was set at 3 s for the first 3 trials; upon a correct response, the stimulus duration for the following trial was shortened one step in the series down to a minimum of 0.1 s, whereas an incorrect response or omission lengthened the stimulus duration one step. Premature responses did not change the stimulus duration on the subsequent trial. A limited hold was present such that the animal always had 3 s to make a response (i.e., 0.5 s stimulus duration + 2.5 s limited hold). The ITI remained constant (5 s) for all trials. Each animal completed a single 30 min session of the titration 5-CSRTT. For each session, the following behavioral endpoints were calculated and defined as follows: (i) Accuracy (i.e., correct responses/total trials); (ii) Omissions (i.e., omitted trials/total trials); (iii) Correct Response Latency (i.e., latency to make a correct response following stimulus presentation); (iv) Response Accuracy (i.e., correct responses/correct + incorrect responses) and (v) Premature Response Rate (i.e., premature responses/correct + incorrect + premature responses).
Statistical analysis
All data are presented as means ± SEM. Data were analyzed using two-way analysis of variance (ANOVA) with genotype and sex as independent factors, followed by appropriate Bonferroni post-hoc analysis in view of statistically significant interactions. In cases where the Levene’s test of homogeneity was significant the effects of genotype were assessed using non-parametric Kruskal–Wallis tests. Temporal locomotor and sleep data were analyzed using three-way repeated measures (RM) ANOVA (i.e., factors: genotype, sex and time). Homogeneity of covariance was assessed using the Mauchly’s test of sphericity; in cases where sphericity was violated the RM ANOVA F was modified according to the Greenhouse–Geisser correction to make it more conservative. Performance in the FR1 Session of the 5-CSRTT was analyzed using Kaplan-Maier survival analysis followed by Mantel-Cox Log Rank pairwise comparisons. The IBM SPSS Statistics for Windows, Version 24.0 (Armonk, NY: IBM Corp.) and the GraphPad Prism version 8.0.0 for Windows (GraphPad Software, La Jolla, CA USA) were used for statistical analysis and graph plotting. Statistical significance was defined as p ≤ 0.05. Detailed statistical analyses are reported in Supplementary Table S1.
Results
PLN is expressed in the GABAergic TRN neurons
In a first set of experiments, we investigated the expression of PLN in the mouse brain (Fig. 2). We found Pln mRNA to be expressed at relatively low levels in the adult mouse brain using RT-PCR (Fig. 2a). Follow-up IHC analysis showed PLN protein expression to be confined in the GABAergic GAD67+ neurons of the TRN (Fig. 2bc) and for PLN to colocalize with SERCA2 in the adult mouse TRN (Fig. 2d). In our experimental setup we did not detect PLN immunoreactivity in other brain regions, including the striatum (STR), thalamus (TH), prefrontal cortex (PFC), hippocampus (HIPP), and cerebellum (CER) (Fig. 3), or in other cell types (e.g., astrocytes; Supplementary Fig. S1) indicating that PLN may possibly play a unique role in the GABAergic TRN neurons of the mouse brain.
Constitutive global loss of PLN induces behavioral deficits in mice
Upon confirming that PLN is expressed at the protein level in the TRN, we employed an existing global Pln−/− knockout mouse model to assess whether genetic ablation of Pln induces gross behavioral effects at the organismal level. Locomotor analysis in the OFT revealed that Pln−/− mice were hyperactive, as compared to their Pln+/+ littermates (Fig. 4a) and spent significantly more time traveling in the center of the OFT arena, indicative of anxiolysis (Fig. 4b). Moreover, genetic Pln ablation affected mouse performance in the Y-maze test indicating that Pln−/− mice present a deficit in spatial working memory (Fig. 4c). Even though global Pln deletion did not significantly affect object location memory (OLM), as both Pln−/− and Pln+/+ littermates spent significantly more % time interacting with the displaced object during the testing phase of the OLM test (Fig. 4d), it significantly impacted object recognition memory (ORM), as Pln−/− mice spent almost equal time exploring the novel and the familiar objects during the testing phase of the ORM (Fig. 4e). Analysis of social exploratory behavior showed that overall global Pln deletion did not significantly affect sociability (Fig. 4f) and preference for social novelty in mice (Fig. 4g). The results of the statistical analyses are reported in Supplementary Table S1.
Conditional Pln deletion in TRN neurons results in hyperactivity
To further explore the TRN-dependent functions of PLN, we generated (Fig. 1) and validated a novel Cre-LoxP conditional knockout (cKO) mouse model with a targeted deletion of Pln in GABAergic TRN neurons (Fig. 5). Given that our immunohistochemical data revealed that expression of the PLN protein in the mouse brain is restricted to GABAergic neurons of the TRN, we selected a well-defined GABAergic marker of TRN neurons (i.e., GAD2), as our Cre driver mouse line in order to selectively ablate PLN protein expression in TRN neurons while leaving expression in other tissues such as cardiac, skeletal, and smooth muscle intact18,21. Using double fluorescent IHC we confirmed that the Pln gene is effectively deleted in the brain’s TRN, as assessed by the absence of PLN immunoreactivity in the GAD67+ TRN neurons in cKO mice (Fig. 5a). We also confirmed that conditional deletion of PLN in the GABAergic TRN neurons was restricted to the TRN of the mouse brain, as peripheral PLN protein expression in the heart of cKO mice was similar to their control littermates (Fig. 1g). Notably, analysis of locomotor activity in the OFT showed that the conditional loss of PLN function in the TRN induces hyperactivity in cKO mice (Fig. 5b,c), further confirming our findings in Pln−/− mice and consolidating a role for PLN in regulating locomotor activity. Mouse performance in the Y-maze was not affected in cKO mice suggesting that unlike global Pln deletion, TRN-specific loss of PLN function does not impact spatial working memory (Fig. 5d). The results of the statistical analyses are reported in Supplementary Table S1.
Loss of PLN function in the TRN affects sleep architecture and impulsivity in cKO mice
EEG/EMG polysomnography for the analysis of sleep architecture revealed that conditional deletion of Pln in the TRN resulted in pronounced alterations in sleep architecture that were confined in the dark period of the cycle (Fig. 6). Specifically, cKO mice spent less time in wakefulness (Fig. 6d) and greater time in NREM (Fig. 6e) and REM sleep (Fig. 6f) during the dark period, as compared to their control littermates. Moreover, cKO mice also had shorter wakefulness bouts in the dark period (Fig. 6d), a higher number of NREM bouts in the dark period (Fig. 6e), and longer REM bouts in the dark period as compared to the control mice (Fig. 6f). cKO mice also exhibited increased transitions between wakefulness and NREM, (Fig. 6g,h) and a trend towards increased incidence of arousals during NREM sleep (Fig. 6i) during the dark period. The same analysis for REM sleep in the dark period revealed that cKO mice spent more time in REM sleep as compared to their control counterparts; post-hoc analysis indicated that the prolonged REM sleep duration during the dark period was more pronounced in female mice. Sirenia EEG spectral analysis did not reveal statistically significant differences between the normalized EEG power density spectral profiles of control and cKO mice (Supplementary Table S2). Behavioral analysis in the 5-CSRTT indicated that conditional loss of PLN function in the TRN affects executive functioning and results in impulsivity. Specifically, in the FR1 session, a Kaplan-Maier survival analysis followed by Mantel-Cox Log Rank pairwise comparisons revealed that female cKO mice presented a delay in reaching the FR1 criterion as compared to the other genotypes indicating that conditional loss of PLN in the TRN affects the initial learning acquisition rate of female mice during FR1 testing of the 5-CSRTT; however, despite this initial deficit, all mice eventually acquired the task (Fig. 7a,b). No statistically significant differences were observed in the PR (Fig. 7c) and the main 5-CSRTT sessions (data not shown). Most importantly, in the final and more challenging “Titration” 5-CSRTT session, attention was not affected in cKO mice (Fig. 7d–i), but overall cKO mice displayed an impulsive phenotype as evidenced by a trend for increased premature response rate (Fig. 7j) and increased number of premature responses (Fig. 7k). The results of the statistical analyses are reported in Supplementary Table S1.
Discussion
This is the first study to investigate the role of PLN in brain and behavior. Herein, we report that PLN is expressed in the TRN of the adult mouse brain and that loss of PLN function results in robust behavioral deficits, including hyperactivity, impulsivity and alterations sleep architecture in mice.
Despite the fact that the SERCA2 is ubiquitously expressed in neurons8,29,30, earlier Northern blotting studies had reported that the Pln gene is not expressed at the mRNA level in the brain of humans, pigs, or mice31,32,54. More recently, a number of high-throughput global RNA-seq and genome-wide microarray gene expression profiling studies have detected Pln mRNA in individual mouse dorsal root ganglion (DRG) neurons, immunopanning-isolated mouse and human cortical neural cells, including astrocytes and neurons, as well as in fetal human primary astrocytes55,56,57,58, supporting the expression of PLN in neural tissues. In our experimental setup, low Pln mRNA levels were detected in the whole mouse brain (Fig. 2a), whereas detection of PLN protein expression was exclusively confined in the GABAergic neurons of the TRN in wild-type Pln+/+ mice (Fig. 2b), but not in their Pln−/− knockout counterparts (Fig. 2c). As theoretically expected, we also confirmed that PLN protein expression co-localizes with SERCA2 in the mouse TRN neurons (Fig. 2d). Importantly, PLN immunoreactivity was exclusively observed in GABAergic TRN neurons but not in other brain regions studied (i.e., the striatum, the prefrontal cortex, the thalamus, the hippocampus, and the cerebellum) (Fig. 3) or in other cell types, (i.e., astrocytes; Fig. S1). Of note, our fluorescent immunohistochemistry data corroborate recent bulk RNA-seq studies reporting higher Pln mRNA expression in the mouse thalamus59, and the TRN-specific PLN mRNA expression pattern observed by in situ hybridization histochemistry (ISH) in the Allen Mouse Brain Atlas [e.g., Exp: 69116799 (sagittal) & Exp: 74357563 (coronal)]60. Taken together, our findings indicate that PLN protein is selectively expressed in the GABAergic TRN neurons and provide first evidence that the PLN/SERCA2 pathway operates in the central nervous system where it possibly plays a unique role in regulating intracellular Ca2+-handling in the TRN neurocircuitry.
TRN dysfunction has been proposed to underlie hyperactivity, deficits in executive functioning, and sleep disturbances5 and to comprise a circuit endophenotype in neurodevelopmental disorders, including autism spectrum disorder (ASD), schizophrenia and attention-deficit/hyperactivity disorder (ADHD) (for review see61). Thus, selective expression of PLN in the TRN further suggests that the PLN/SERCA2 pathway is of critical importance in regulating the dynamics of the TRN neurocircuitry and fundamental behavioral processes of great biological significance. Indeed, compelling evidence suggests that SERCA plays a critical role in brain physiology and pathophysiology; SERCA2 dysregulation has been associated with several neuropsychiatric disorders, including schizophrenia, bipolar disorder, cerebral ischemia, alcoholism, as well as Alzheimer’s and Parkinson’s diseases (for review see17,62). Interestingly, recent studies from our group have revealed that chronic pharmacological stimulation of SERCA induces anxiety- and depressive-like behaviors, memory deficits, as well as sustained brain region-specific noradrenergic and serotonergic neurochemical alterations in mice, further highlighting the importance of dissecting the neurobiological implications of SERCA2-dependent Ca2+ regulatory mechanisms in the brain51,39.
Interestingly, TRN-specific dysfunction has been linked to an ADHD-like hyperactive phenotype in mice as TRN-targeted genetic ablation of the patched domain containing 1 (Ptchd1) gene results in locomotor hyperactivity63. Thus, upon confirming the expression of PLN in the mouse TRN, we further sought to assess the gross behavioral consequences of global genetic ablation of Pln using an existing Pln knockout mouse model26,33. Interestingly, constitutive deletion of the Pln gene resulted in hyperactivity and cognitive deficits in Pln−/− mice as they traveled a greater distance in the OFT (Fig. 4a), and were outperformed by their Pln+/+ counterparts in the Y-maze and the NOR tests, with these behavioral deficits being indicative of impaired spatial working memory and long-term object recognition memory, respectively (Fig. 4c–e). The behavioral deficits observed upon global genetic Pln ablation were not accompanied by alterations in sociability, as assessed in the three-compartmental sociability test (Fig. 4fg), supporting the notion that PLN is not strongly implicated in this behavioral domain.
Upon finding that PLN immunoreactivity is restricted to the GABAergic TRN neurons in the adult mouse brain and conducting an initial behavioral screening of global Pln−/− mice, we employed a TRN-specific cKO model to assess how loss of PLN function affects behaviors that specifically map onto thalamic reticular circuits (i.e., locomotor activity, sleep and executive functioning). It is well established that the TRN regulates important brain-wide functions, including sensorimotor processes64, executive functioning63 and the generation of sleep rhythms65. To further explore the TRN-dependent functions of PLN, we generated and validated a novel conditional cKO mouse line with diminished PLN expression in the GABAergic TRN neurons. Indeed, we found that conditional deletion of Pln in the TRN disturbed thalamic reticular-dependent behaviors. Specifically, much like their Pln−/− 129 Sv counterparts, cKO C57 mice were hyperactive (Fig. 5bc), while deletion of the Pln gene in TRN neurons was associated with impulsivity, as observed in the titration session of the 5-CSRTT (Fig. 7j,k). As no differences were observed in reward collection latency (data not shown) nor correct response latency (Fig. 7g), the hyperactive phenotype of cKO mice does not significantly confound the interpretation of the 5-CSRTT data66. Despite the fact that cKO mice did not exhibit deficits in spatial working memory in the Y-maze, female cKO mice presented a delay in reaching the FR1 criterion in the 5-CSRTT (Fig. 7ab) indicating that conditional loss of PLN function in the TRN may result in an initial sex-specific associative learning deficit in acquiring this operant conditioning task, without this deficit however significantly affecting attentional processing in the subsequent phases of the 5-CSRTT52. Notably, the TRN is critical for the generation of sleep spindles and NREM sleep65,67,68. Thus, it is conceivable that deregulation of SERCA2-dependent Ca2+ signaling mechanisms may impact thalamocortical network oscillations relevant for NREM sleep and overall sleep architecture. Present data revealed that cKO mice exhibited significantly increased amounts of NREM and REM sleep, at the expense of wakefulness, throughout the dark period (Fig. 6d–f). In line with these findings, bout analysis of the dark period revealed cKO mice exhibited more NREM and REM bouts, enhanced REM bout length, and reduced wakefulness bout length (Fig. 6d–f). Interestingly, previous studies have reported fragmented sleep architecture in mice with ADHD-like behavioral features63, while genetic ablation of CaV2.3 Ca2+ channels, that are predominantly expressed in the TRN, has been reported to result in increased NREM sleep duration at the expense of wakefulness in CaV2.3KO mice69. As TRN neuronal firing patterns have been shown to directly mediate the transition between wakefulness and NREM70, we hypothesize that a potential mechanism for these sleep abnormalities in cKO mice may include aberrations in Ca2+-mediated TRN neuronal firing due to loss of PLN, enhancing wakefulness-NREM transition-promoting TRN circuits.
Notably, locomotor hyperactivity upon loss of PLN function was a robust behavioral finding that was evidenced in both global 129S Pln−/− and conditional C57 cKO mice underscoring the role of PLN in regulating locomotor activity though the thalamic reticular neurocircuit. However, in our experimental setup the spatial working memory Y maze deficits observed in Pln−/− mice were not recapitulated in cKO mice. It is noteworthy that differences between the behavioral profiles observed between the global Pln−/− and the conditional cKO mice could be attributed, at least in part, to the peripheral effects of PLN in other PLN-expressing tissues and/or cell types (e.g., myocardium, skeletal and smooth muscle cells), and/or to the different mouse strains used for Pln−/− and cKO mice; indeed, there are noted behavioral differences between the C57 and the 129S strains71,72 that could differentially impact mouse behavior upon loss of PLN. Interestingly, a novel subcortical GABAergic neural input pathway was recently discovered that originates in the TRN and projects to striatal parvalbumin (PV) interneurons73. Theoretically, excitation of this TRN projection would result in inhibition of striatal PV interneurons and concomitant disinhibition of the striatal spiny projection neurons (SPNs)73 that comprise as much as 95% of the total striatal neuron population74, thus providing a putative subcortical pathway by which the TRN could directly affect striatum-dependent behaviors, such as locomotor activity and executive functioning. Interestingly, in our experimental setup the core behavioral consequences observed upon loss of PLN function were not sex-differentiated; however, two peripheral behavioral measures (i.e., time to reach FR1 criterion in the 5-CSRTT and REM sleep) appeared to be differentially affected between male and female cKO mice underscoring the need for future studies to employ both sexes when assessing the effects of PLN in the brain. Studies from our lab are underway to interrogate the role of the PLN/SERCA2 pathway in specific components of the TRN neurocircuitry, to investigate the effect of Pln ablation on thalamic reticular Ca2+ dynamics and electrophysiological neuronal properties in order to dissect the cellular and molecular mechanisms pertaining to the role of PLN in the TRN.
Interestingly, in contrast to Pln−/− mice, human PLN-null mutation carriers eventually develop cardiac pathology suggesting that the function of PLN may be different in the heart of mice and humans, or that PLN’s cardiac function is similar in mice and humans but loss-of-function PLN mutations, that are deleterious for humans, are not for mice; the latter could be partly attributed to species-specific differences in cardiac reserve and regulation of Ca2+-homeostasis in murine and human cardiomyocytes21. Thus, our findings may also have clinical neurobiological implications for carriers of human PLN mutations, including the L39stop PLN null mutation that leads to lethal dilated cardiomyopathy18,21,75.
Conclusions
Overall, our study shows that loss of PLN function in the TRN results in aberrant thalamic reticular behavioral phenotypes in mice (i.e., hyperactivity, impulsivity and sleep deficits). Present data exposed a novel role for PLN in the brain, and further suggest that PLN is a critical regulator of SERCA2 in the TRN neurocircuitry; the intricate molecular mechanisms orchestrated by the PLN/SERCA2 pathway in the TRN are currently under investigation by our lab. As disruption of the TRN-thalamocortical circuits have been implicated in the pathophysiology of debilitating brain diseases, including schizophrenia, ADHD and ASD61,63,76, gaining insights into the regulation of Ca2+ homeostasis in the TRN may hold great promise for the discovery of innovative pharmacotherapeutic targets for the prevention and treatment of these devastating neurodevelopmental disorders.
References
Pinault, D. The thalamic reticular nucleus: Structure, function and concept. Brain Res. Brain Res. Rev. 46, 1–31 (2004).
Houser, C. R., Vaughn, J. E., Barber, R. P. & Roberts, E. GABA neurons are the major cell type of the nucleus reticularis thalami. Brain Res. 200, 341–354 (1980).
Cueni, L. et al. T-type Ca2+ channels, SK2 channels and SERCAs gate sleep-related oscillations in thalamic dendrites. Nat. Neurosci. 11(6), 683–692 (2008).
Huguenard, J. R. & Prince, D. A. A novel T-type current underlies prolonged Ca(2+)-dependent burst firing in GABAergic neurons of rat thalamic reticular nucleus. J. Neurosci. 12, 3804–3817 (1992).
Wells, M. F., Wimmer, R. D., Schmitt, L. I., Feng, G. & Halassa, M. M. Thalamic reticular impairment underlies attention deficit in Ptchd1(Y/-) mice. Nature 532, 58–63 (2016).
Coulon, P. et al. Burst discharges in neurons of the thalamic reticular nucleus are shaped by calcium-induced calcium release. Cell Calcium 46, 333–346 (2009).
Berridge, M. J., Bootman, M. D. & Roderick, H. L. Calcium signalling: Dynamics, homeostasis and remodelling. Nat. Rev. Mol. Cell Biol. 4, 517–529 (2003).
Baba-Aissa, F., Raeymaekers, L., Wuytack, F., Dode, L. & Casteels, R. Distribution and isoform diversity of the organellar Ca2+ pumps in the brain. Mol. Chem. Neuropathol. 33, 199–208 (1998).
MacLennan, D. H., Brandl, C. J., Korczak, B. & Green, N. M. Amino-acid sequence of a Ca2+ + Mg2+-dependent ATPase from rabbit muscle sarcoplasmic reticulum, deduced from its complementary DNA sequence. Nature 316, 696–700 (1985).
Pozzan, T., Rizzuto, R., Volpe, P. & Meldolesi, J. Molecular and cellular physiology of intracellular calcium stores. Physiol. Rev. 74, 595–636 (1994).
Berridge, M. J., Bootman, M. D. & Lipp, P. Calcium: A life and death signal. Nature 395, 645–648 (1998).
Earls, L. R. & Zakharenko, S. S. A synaptic function approach to investigating complex psychiatric diseases. Neuroscientist 20, 257–271 (2014).
Earls, L. R. et al. Age-dependent microRNA control of synaptic plasticity in 22q11 deletion syndrome and schizophrenia. J. Neurosci. 32, 14132–14144 (2012).
Cederlöf, M. et al. The association between Darier disease, bipolar disorder, and schizophrenia revisited: A population-based family study. Bipolar Disord. 17, 340–344 (2015).
Nensa, F. M. et al. Amyloid beta a4 precursor protein-binding family B member 1 (FE65) interactomics revealed synaptic vesicle glycoprotein 2A (SV2A) and sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) as new binding proteins in the human brain. Mol. Cell Proteomics 13, 475–488 (2014).
Klocke, B. et al. Insights into the role of intracellular calcium signaling in the neurobiology of neurodevelopmental disorders. Front. Neurosci. 17, 1093099 (2023).
Britzolaki, A., Saurine, J., Klocke, B. & Pitychoutis, P. M. A role for SERCA pumps in the neurobiology of neuropsychiatric and neurodegenerative disorders. Adv. Exp. Med. Biol. 1131, 131–161 (2020).
MacLennan, D. H. & Kranias, E. G. Phospholamban: A crucial regulator of cardiac contractility. Nat. Rev. Mol. Cell Biol. 4, 566–577 (2003).
Paul, R. J., Shull, G. E. & Kranias, E. G. The sarcoplasmic reticulum and smooth muscle function: Evidence from transgenic mice. Novartis Found Symp. 246, 228–38 (2002).
Kranias, E. G. & Solaro, R. J. Phosphorylation of troponin I and phospholamban during catecholamine stimulation of rabbit heart. Nature 298, 182–184 (1982).
Kranias, E. G. & Hajjar, R. J. The phospholamban journey 4 decades after setting out for Ithaka. Circ. Res. 120, 781–783 (2017).
Vafiadaki, E., Haghighi, K., Arvanitis, D. A., Kranias, E. G. & Sanoudou, D. Aberrant PLN-R14del protein interactions intensify SERCA2a inhibition, driving impaired Ca(2+) handling and arrhythmogenesis. Int. J. Mol. Sci. 23, 6947 (2022).
Wang, H. S. et al. SERCA2a superinhibition by human phospholamban triggers electrical and structural remodeling in mouse hearts. Physiol. Genom. 43, 357–364 (2011).
Rogalska, M. E. et al. Isoform changes of action potential regulators in the ventricles of arrhythmogenic phospholamban-R14del humanized mouse hearts. Metabolism 138, 155344 (2023).
Chu, G. et al. Compensatory mechanisms associated with the hyperdynamic function of phospholamban-deficient mouse hearts. Circ. Res. 79, 1064–1076 (1996).
Luo, W. et al. Targeted ablation of the phospholamban gene is associated with markedly enhanced myocardial contractility and loss of beta-agonist stimulation. Circ. Res. 75, 401–409 (1994).
Chu, G. et al. Proteomic analysis of hyperdynamic mouse hearts with enhanced sarcoplasmic reticulum calcium cycling. FASEB J. 18, 1725–1727 (2004).
Kranias, E. G. & Hajjar, R. J. Modulation of cardiac contractility by the phospholamban/SERCA2a regulatome. Circ. Res. 110, 1646–1660 (2012).
Baba-Aissa, F., Van den Bosch, L., Wuytack, F., Raeymaekers, L. & Casteels, R. Regulation of the sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA) 2 gene transcript in neuronal cells. Brain Res. Mol. Brain Res. 55, 92–100 (1998).
Baba-Aissa, F. et al. Distribution of the organellar Ca2+ transport ATPase SERCA2 isoforms in the cat brain. Brain Res. 743, 141–153 (1996).
McTiernan, C. F. et al. The human phospholamban gene: Structure and expression. J. Mol. Cell Cardiol. 31, 679–692 (1999).
Ganim, J. R. et al. Mouse phospholamban gene expression during development in vivo and in vitro. Circ. Res. 71, 1021–1030 (1992).
Bibli, S.-I. et al. Cardioprotection by H2S engages a cGMP-dependent protein kinase G/phospholamban pathway. Cardiovasc. Res. 106, 432–442 (2015).
Quadros, R. M. et al. Easi-CRISPR: A robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins. Genome Biol. 18, 92 (2017).
Haeussler, M. et al. Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR. Genome Biol. 17, 148 (2016).
Franceschelli, A., Sens, J., Herchick, S., Thelen, C. & Pitychoutis, P. Sex differences in the rapid and the sustained antidepressant-like effects of ketamine in stress-naive and “depressed” mice exposed to chronic mild stress. Neuroscience 290, 49–60 (2015).
Sens, J. et al. Lipopolysaccharide administration induces sex-dependent behavioural and serotonergic neurochemical signatures in mice. Pharmacol. Biochem. Behav. 153, 168–181 (2017).
Thelen, C., Sens, J., Mauch, J., Pandit, R. & Pitychoutis, P. M. Repeated ketamine treatment induces sex-specific behavioral and neurochemical effects in mice. Behav. Brain Res. 312, 305–312 (2016).
Britzolaki, A., Cronin, C. C., Flaherty, P. R., Rufo, R. L. & Pitychoutis, P. M. Chronic but not acute pharmacological activation of SERCA induces behavioral and neurochemical effects in male and female mice. Behav. Brain Res. 399, 112984 (2021).
Pitychoutis, P. M., Pallis, E. G., Mikail, H. G. & Papadopoulou-Daifoti, Z. Individual differences in novelty-seeking predict differential responses to chronic antidepressant treatment through sex- and phenotype-dependent neurochemical signatures. Behav. Brain Res. 223, 154–168 (2011).
Belarbi, K. et al. Beneficial effects of exercise in a transgenic mouse model of Alzheimer’s disease-like Tau pathology. Neurobiol. Dis. 43, 486–494 (2011).
Kraeuter, A.-K., Guest, P. C. & Sarnyai, Z. The Y-maze for Assessment of Spatial Working and Reference Memory in Mice. Pre-Clinical Models 105–111 (Springer, 2019).
Lalonde, R. The neurobiological basis of spontaneous alternation. Neurosci. Biobehav. Rev. 26, 91–104 (2002).
Vogel-Ciernia, A. & Wood, M. A. Examining object location and object recognition memory in mice. Curr. Protoc. Neurosci. 69, 831 (2014).
Peça, J. et al. Shank3 mutant mice display autistic-like behaviours and striatal dysfunction. Nature 472, 437–442 (2011).
Pitychoutis, P. M., Belmer, A., Moutkine, I., Adrien, J. & Maroteaux, L. Mice lacking the serotonin Htr2B receptor gene present an antipsychotic-sensitive schizophrenic-like phenotype. Neuropsychopharmacology 40, 2764–2773 (2015).
Boutrel, B., Franc, B., Hen, R., Hamon, M. & Adrien, J. Key role of 5-HT1B receptors in the regulation of paradoxical sleep as evidenced in 5-HT1B knock-out mice. J. Neurosci. 19, 3204–3212 (1999).
Wang, Y., He, J., Kastin, A. J., Hsuchou, H. & Pan, W. Hypersomnolence and reduced activity in pan-leptin receptor knockout mice. J. Mol. Neurosci. 51, 1038–1045 (2013).
Léna, C. et al. β2-Containing nicotinic receptors contribute to the organization of sleep and regulate putative micro-arousals in mice. J. Neurosci. 24, 5711–5718 (2004).
Tobler, I., Deboer, T. & Fischer, M. Sleep and sleep regulation in normal and prion protein-deficient mice. J. Neurosci. 17, 1869–1879 (1997).
Klocke, B., Moore, C., Ott, H. & Pitychoutis, P. M. Chronic pharmacological activation of SERCA with CDN1163 affects spatial cognitive flexibility but not attention and impulsivity in mice. Behav Pharmacol. 34(8), 477–487 (2023)
Smith, B. L., Laaker, C. J., Lloyd, K. R., Hiltz, A. R. & Reyes, T. M. Adolescent microglia play a role in executive function in male mice exposed to perinatal high fat diet. Brain Behav. Immun. 84, 80–89 (2020).
McKee, S. E., Grissom, N. M., Herdt, C. T. & Reyes, T. M. Methyl donor supplementation alters cognitive performance and motivation in female offspring from high-fat diet-fed dams. FASEB J. 31, 2352–2363 (2017).
Plessers, L., Eggermont, J. A., Wuytack, F. & Casteels, R. A study of the organellar Ca2(+)-transport ATPase isozymes in pig cerebellar Purkinje neurons. J. Neurosci. 11, 650–656 (1991).
Zhang, Y. et al. An RNA-sequencing transcriptome and splicing database of glia, neurons, and vascular cells of the cerebral cortex. J. Neurosci. 34, 11929–11947 (2014).
Zhang, Y. et al. Purification and characterization of progenitor and mature human astrocytes reveals transcriptional and functional differences with mouse. Neuron 89, 37–53 (2016).
Sengupta, A. et al. Gene expression profiling of human primary astrocytes exposed to manganese chloride indicates selective effects on several functions of the cells. Neurotoxicology 28, 478–489 (2007).
Li, C. L. et al. Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity. Cell Res. 26, 967 (2016).
Uhlén, M. et al. Tissue-based map of the human proteome. Science 347, 1260419 (2015).
Allen Institute for Brain Science. Allen Mouse Brain Atlas. mouse.brain-map.org (Allen Institute for Brain Science, 2004).
Krol, A., Wimmer, R. D., Halassa, M. M. & Feng, G. Thalamic reticular dysfunction as a circuit endophenotype in neurodevelopmental disorders. Neuron 98, 282–295 (2018).
Britzolaki, A., Saurine, J., Flaherty, E., Thelen, C. & Pitychoutis, P. M. The SERCA2: A gatekeeper of neuronal calcium homeostasis in the brain. Cell Mol. Neurobiol. 38, 981–994 (2018).
Wells, M. F., Wimmer, R. D., Schmitt, L. I., Feng, G. & Halassa, M. M. Thalamic reticular impairment underlies attention deficit in Ptchd1Y/− mice. Nature 532, 58–63 (2016).
Erlij, D. et al. Dopamine D4 receptor stimulation in GABAergic projections of the globus pallidus to the reticular thalamic nucleus and the substantia nigra reticulata of the rat decreases locomotor activity. Neuropharmacology 62, 1111–1118 (2012).
Halassa, M. M. et al. State-dependent architecture of thalamic reticular subnetworks. Cell 158, 808–821 (2014).
Fizet, J., Cassel, J. C., Kelche, C. & Meunier, H. A review of the 5-Choice Serial Reaction Time (5-CSRT) task in different vertebrate models. Neurosci. Biobehav. Rev 71, 135–153 (2016).
Astori, S., Wimmer, R. D. & Luthi, A. Manipulating sleep spindles–expanding views on sleep, memory, and disease. Trends Neurosci. 36, 738–748 (2013).
Dang-Vu, T. T., McKinney, S. M., Buxton, O. M., Solet, J. M. & Ellenbogen, J. M. Spontaneous brain rhythms predict sleep stability in the face of noise. Curr. Biol. 20, R626–R627 (2010).
Siwek, M. E. et al. The CaV2.3 R-type voltage-gated Ca2+ channel in mouse sleep architecture. Sleep 37, 881–92 (2014).
Liu, H. et al. Microglia modulate stable wakefulness via the thalamic reticular nucleus in mice. Nat. Commun. 12, 4646 (2021).
Wolff, M., Savova, M., Malleret, G., Segu, L. & Buhot, M. C. Differential learning abilities of 129T2/Sv and C57BL/6J mice as assessed in three water maze protocols. Behav. Brain Res. 136, 463–474 (2002).
Paulus, M. P., Dulawa, S. C., Ralph, R. J. & Mark, A. G. Behavioral organization is independent of locomotor activity in 129 and C57 mouse strains. Brain Res. 835, 27–36 (1999).
Klug, J. R. et al. Differential inputs to striatal cholinergic and parvalbumin interneurons imply functional distinctions. Elife 7, 35657 (2018).
Gerfen, C. R. & Surmeier, D. J. Modulation of striatal projection systems by dopamine. Annu. Rev. Neurosci. 34, 441–466 (2011).
Haghighi, K. et al. Human phospholamban null results in lethal dilated cardiomyopathy revealing a critical difference between mouse and human. J. Clin. Invest. 111, 869–876 (2003).
Young, A. & Wimmer, R. D. Implications for the thalamic reticular nucleus in impaired attention and sleep in schizophrenia. Schizophr. Res. 180, 44–47 (2017).
Acknowledgements
The authors would like to thank the undergraduate student members of the Pitychoutis lab, and specifically Emily Flaherty, Kaitlyn Martin, Augustine Miller and Jason Tornes, for assisting with preliminary behavioral experiments and/or with mouse colony maintenance, and Dr. Dimitrios Arvanitis (Biomedical Research Foundation of the Academy of Athens, Athens, Greece) for his insights into the mRNA assessments. We would like to thank Dr. Hu and the members of the Transgenic Animal and Genome Editing Core at Cincinnati Children’s Hospital Medical Center (CCHMC, Cincinnati, OH) for their service in generating the floxed PLN mouse. The authors would also like to thank Dr. James P. Herman (University of Cincinnati, Cincinnati, OH) for his valuable advice and support with the construction of the conditional mouse line, as well as Dr. Teresa Reyes (University of Cincinnati, Cincinnati, OH) and the staff at Lafayette Instrument (IN, USA) for their support with the 5-CSRTT task.
Funding
BK, AB and CTh were supported by the University of Dayton (UD) Graduate School and by the UD Office for Graduate Affairs through the Graduate Student Summer Fellowship (GSSF) Program. KK was supported by the UD Graduate School and by the Department of Biology. JS was supported by a Barry Goldwater Scholarship Award, by the UD Honors Program, by a Lancaster-McDougall award from the Department of Biology, a College of Arts & Sciences (CAS) Dean’s Summer Research fellowship and by UD’s STEM Catalyst Grant program. KB was supported by CAS Dean’s Summer Research fellowships. HO was supported by the UD Honors Program, a CAS Dean’s Summer Research fellowship, and a Lancaster-McDougal departmental fellowship. DS and EGK were supported by a grant from the Leducq Foundation for Cardiovascular Research (CURE-PLaN, 18CVD01). This work was supported by funding from the National Institute of Neurological Disorders and Stroke (NINDS) of the National Institutes of Health (NIH) under award number R03NS109836, as well as by a UD inaugural STEM Catalyst grant, UD Research Council SEED Grants and start-up funding from UD to PMP. Funding sponsors had no further role in study design, in the collection, analysis and interpretation of data, in the writing of the report, and in the decision to submit the article for publication. The construction of the floxed PLN mouse line was funded by an Inaugural STEM Catalyst grant from the University of Dayton to Dr. Pitychoutis.
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B.K. and A.B. contributed equally. Drafting of the first manuscript: B.K., P.M.P. Acquisition, analysis, and/or interpretation of data: B.K., A.B., J.S., H.O., K.K., K.B., C.Th., C.Tz., D.S., E.G.K., P.M.P. Study design, supervision and funding acquisition: P.M.P. All authors reviewed and approved the final version of the manuscript.
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Klocke, B., Britzolaki, A., Saurine, J. et al. A novel role for phospholamban in the thalamic reticular nucleus. Sci Rep 14, 6376 (2024). https://doi.org/10.1038/s41598-024-56447-x
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DOI: https://doi.org/10.1038/s41598-024-56447-x
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