Abstract
The invasion of ecosystems by non-native species is recognized as one of the most significant global challenges, particularly in semiarid regions where native biodiversity is already under stress from drought and land degradation. The implicit assumption is that invaders are strong competitors, but a greenhouse pairwise experiment conducted to examine intraspecific and interspecific competition effects of Opuntia ficus-indica, a widespread invader in semiarid ecosystems, with two species native to the highlands of Eritrea, Ricinus communis and Solanum marginatum, revealed that O. ficus-indica is a weak competitor. The unique ability of O. ficus-indica’s fallen cladodes to undergo vegetative growth becomes a fundamental trait contributing to its spread. This growth strategy allows O. ficus-indica to outgrow native species and establish a significant presence. In direct interaction, the competition in aboveground productivity measured by the logarithmic response ratio for O. ficus-indica was 3.4-fold and 5.9-fold higher than for R. communis and S. marginatum, respectively. Belowground, the native R. communis was facilitated (− 1.00 ± 0.69) by O. ficus-indica which itself suffered from high competition. This pattern became even more evident under water shortage, where aboveground competition for S. marginatum decreased 5.7-fold, and for O. ficus-indica, it increased 1.4-fold. Despite being a poor competitor, O. ficus-indica outperformed R. communis and S. marginatum in both aboveground (4.3 and 3.8 times more) and belowground (27 and 2.8 times more) biomass production, respectively. The findings of this study challenge the common interpretation that invasive species are strong competitors and highlight the importance of considering other factors, such as productivity and tolerance limits when assessing the potential impacts of invasive species on semiarid ecosystems.
Similar content being viewed by others
Introduction
Invasive plants are often found to outcompete native species during their successful invasions of many ecosystems1. Thereby, invasive species can endanger populations of native species2,3,4, reduce spatial diversity5, or negatively affect crop yields6. All of these processes are commonly explained by the superior competitive abilities of the invasive species7. Moreover, the ability of the invasive species to adapt to a new environment can be influenced, either positively or negatively, by the presence of other species8. Some argue that only highly competitive species can spread in a new environment9 after successfully overcoming biotic and abiotic barriers10.
Field observations show that invasive species outcompete and limit the abundance of native species7,11,12 and direct paired competition experiments generally support this finding7. Exceptions with highly competitive native species limiting the exotic invaders mainly stem from temperate grasslands7. Here, competition by native species has been shown to reduce invasive plant growth considerably and has an even stronger effect than herbivory. Accordingly, the high competitive abilities of native species can effectively reduce invasion13,14,15. Although some communities or ecosystems are more susceptible to invasions than others, there is limited understanding of the competitive balance between invaders and native species in subtropical semiarid shrublands7, where unpredictable precipitation can cause strong abiotic stress as well as temporarily abundant available resources.
The susceptibility of communities to invasion is often increased by the presence of available resources due to fluctuating environments in time or disturbances in space16,17. The probability of an invasion occurring is closely linked to the resource availability during the invasion period, and this availability is, in turn, impacted by the degree of disturbance in that particular ecosystem16. Consequently, ecosystems in unpredictable climates where a limiting resource, here water, creates chronic stress but intermittent rainfall events causing unlimited availability at hardly predictable points in time, can be expected to offer little resistance to invasions. Subtropical semiarid drylands are such ecosystems, but little is known about invasion processes and competitive balances between invaders and native species in these ecosystems7.
Another factor that plays an important role in invasion success is stress tolerance, the ability of a species to withstand and cope with various abiotic and biotic stress factors, such as drought, temperature extremes, or competition for resources18. As a result, this tolerance has the potential to further affect the competitive balances, particularly in resource-limited ecosystems such as the water-limited semi-arid subtropical shrublands. In the context of plant competition, water availability plays an important role in affecting the level of competition and the mechanisms plants use to compete for resources19. For instance, limitation of available water may cause plants to increase their root growth to access more water, which can eventually intensify competition for water and nutrients in the soil20. Additionally, water stress can impair plant growth and increase the proportion of visibly wilted leaves, affecting the overall health and competitive ability of the plants21,22. The highly water-efficient CAM (Crassulacean Acid Metabolism) photosynthetic strategy could benefit invasive species in such an environment as a so-called ‘novel weapon’23,24.
Opuntia ficus-indica (prickly pear) is a cactus native to Mexico which is exceptionally successful in invading arid and semi-arid ecosystems, e.g., in Australia, South Africa, Kenya, Tanzania, Ethiopia, Eritrea, Somalia, Yemen, North America, and Hawaii25,26,27. Moreover, it is reported that O. ficus-indica is the most widespread invasive cactus, which has been found nowadays in 22 different countries on all continents except Antarctica outside its native range28. It is reported to alter the composition of the indigenous plant and animal communities29 and to reduce spatial diversity5. Its invasion has economic effects by impeding the movement of livestock and humans, as it forms impenetrable thickets and thereby threatens large-scale cattle ranching26. The spiny nature of O. ficus-indica impedes browsing and grazing, and in heavily infested areas, livestock struggle to access grazing areas. This negatively impacts cattle ranching, reducing livestock numbers and harming the local economy30,31. Despite studies recognizing the utilization and grazing barriers of the cactus32,33, we are unaware of clear data on the extent of the economic effects. Furthermore, O. ficus-indica capitalizes on its important high water use efficiency as a CAM plant. This adaptation not only grants drought tolerance but also empowers the plant to thrive in arid conditions, enabling survival with minimal precipitation34,35.
Based on its invasion success, O. ficus-indica is assumed to outcompete the neighbouring native species36 and, due to its strong drought tolerance37,38, can become even more competitive when water availability is scarce. Coincidentally, projections of precipitation in vast parts of the areas invaded by O. ficus-indica, with ongoing climate change, will face increasing droughts in future, leading to water scarcity39. The competitive abilities of O. ficus-indica, however, have not yet been tested experimentally, neither under water-limited nor under wetter conditions.
Accordingly, this paper deals with pairwise competition experiments in two different water availabilities between the invasive plant O. ficus-indica and two typical and common native plant species of invaded areas in Eritrea, Ricinus communis and Solanum marginatum. Considering its global invasion success in dry ecosystems, we hypothesized that O. ficus-indica has a high competitive power and outcompetes the native species irrespective of water availability. We furthermore expected that its competitive superiority increases under water stress.
Materials and methods
Competitive balance between the invasive O. ficus-indica and two species native to the highlands of Eritrea, Ricinus communis and Solanum marginatum, was experimentally evaluated. All three species grow together in the highlands of Eritrea5 at altitudes above 1500 m with a mean annual rainfall of 500 mm. It is an area with a warm to cool semiarid climate and potential evapotranspiration ranging between 1300 and 1800 mm. In this area, the rainy season normally lasts about three months, beginning in June and ending in August. Besides heavy rain, occasional showers come in March and April5,46. All three plant species/ seeds were collected from the highlands of Eritrea in 2018 with the permission obtained from the Ministry of Agriculture, Regulatory Services Department with a certificate issue number ER-PSC-00026. The species were identified following Hedberg and Edwards47, Edwards et al.48,49,50, Hedberg et al.51,52 Mesfin53, Bein et al.54, and by comparing the collected specimens at the Herbarium of the Eritrea Institute of Technology. The plant collection and use were in accordance with all the relevant guidelines.
Study species
Vilà et al.6 and Vilà and Weiner7 criticise pairwise competition experiments between invasive and native species for selecting highly competitive and aggressive invaders and comparing them to rare and threatened native species, which, per se, are poor competitors. Here, we avoid this bias by comparing a globally successful invasive species to two common and widespread native species that overlap in range and are known to be tolerant to disturbance.
Opuntia ficus-indica (L.) Mill. (Cactaceae) is an evergreen perennial plant that can grow up to 5 m in height. The species has succulent stems that are formed as a sequence of flattened segments, the cladodes, which generally have elliptical bases that supports the greatly enlarged, flattened upper portions. O. ficus-indica has spines, morphologically corresponding to leaves. Its flowers (5–10 cm in diameter) are sessile and solitary, and the fruits are berries that are 4–8 cm in diameter55,56. Nieddu and Chessa56 found the germination of O. ficus-indica seeds reaching up to 90% in growth chambers with a day/night temperature of 30/20 °C, but only reaching 55% when seeds were kept at room temperature and 43% when seeds were placed outdoors. The seeds are usually dispersed after the consumption of the fruits by humans, birds, and other animals (endozoochory). The seeds require comparatively long time for germination due to their hard, lignified integuments which need to be overcome by physical or chemical reactions57. Furthermore, vegetative propagation occurs through cladodes readily taking root upon falling to the ground and conspicuous patch formation is an important factor in the persistence of local populations of the plant, although seedling recruitment is essential for expanding the geographic range and establishment in new areas55.
Ricinus communis L. (Euphorbiaceae) is a fast-growing, soft woody shrub or small tree (up to 5 m tall) and utilizes the C3 photosynthetic pathway. R. communis is indigenous to eastern Africa, the south-eastern Mediterranean Basin, and India, and it is commonly distributed throughout the tropics and warm temperate regions. It has developed various strategies, such as rapid growth, allelopathy, thriving in a wide range of soil conditions, and high seed production, to adapt to the conditions of disturbed areas58. R. communis is also known as a poisonous plant due to the presence of toxic ricin and ricinine in its seeds and other parts, however, R. communis is still commonly used as an ornamental plant and for its antimicrobial features, it is used as a medicinal plant to treat several ailments59.
Solanum marginatum L. (Solanaceae), native to the highlands of Eritrea and Ethiopia, is a perennial shrub that follows the C3 photosynthetic pathway. It can grow up to 2 m tall and its leaves are densely covered in white stellate hairs and armed on both the upper and lower surfaces with prickles60. In its native range, it usually occurs in disturbed areas between altitudes of 2000 m and 3000 m above sea level61,62. S. marginatum is usually unpalatable to herbivores mainly due to the presence of a poisonous alkaloid chemical compound63,64.
These three species have all been referred to as weedy, thrive in disturbed areas58,62,65, and possess chemical and/or physical defences against herbivory. They differ little in their potential plant heights, but in some morphological features; O. ficus-indica, as a cactus is a succulent plant that can store water, unlike the other two native species. Sharing a similar life strategy and avoiding the common bias of choosing rare native species6,7 were the important considerations for choosing these two native species.
Experimental design
The competition between species can be quantified experimentally using indices based on pairwise experiments which express competition intensity, effects, and response3,40. Competition indices help to quantify the proportional decrease in native plant performance due to the competing effects of invasive species and compare the effects on different species or under different environmental conditions40,41,42,43. When interspecific competition is weaker than the intraspecific competition in an invaded ecosystem, each native species in that community limits its own population growth more than it limits that of the competitive invader44,45.
The greenhouse competition experiment was carried out from February 2020 to May 2021 at Greifswald, Germany. Two species native to the highlands of Eritrea, Ricinus communis and Solanum marginatum, were selected to test the competitive potential of the invasive cactus, O. ficus-indica in a common-garden pairwise competition experiment. Two different treatments were set up based on resource availability (water), depicting dry and wet environments. Each treatment was prepared with fifty pots with a volume of one litre. The substrate was a mixture of 75% loamy forest soil and 25% quartz sand. All plants were raised from seeds. Since O. ficus-indica needed more time to germinate, it was sown in January 2019, eight months ahead of the other two plants, which were sown in August 2019. After being transplanted into their respective target pots on February 10th, 2020, all plants, regardless of the species, exhibited similar heights of approximately 15 cm and no conspicuous difference in belowground biomass (personal observation). The plants were categorized into monocultures of each species (intraspecific competition) and polycultures of each native together with the invasive (interspecific competition). The intraspecific category had a pair of R. communis plants per pot (20 replicates), a pair of S. marginatum plants per pot (20 replicates), and a pair of O. ficus-indica plants per pot (20 replicates). The interspecific category had O. ficus-indica and R. communis plants per pot (20 replicates) and O. ficus-indica and S. marginatum plants per pot (20 replicates). Half of the pots from each category were distributed to each condition of the wet and dry environment. The pots in the wet environment were watered twice per week with 100 ml of water, simulating a wet condition, the other half of the pots were watered only once per week with 100 ml. The watering regimes were based on pre-trials with all three species. The dry variant was set right above the limit at which the native species showed strongly increased mortality. The invader O. ficus-indica proved to be remarkably resilient, surviving for nine months without any watering. To assess the plant's water tolerance limits, we conducted a pot experiment, exposing 16 plants to a gradient of water availability ranging from no water up to 260 ml twice a week over a period of nine months. While the growth of the plants exposed to drought on the lower end of the gradient was impeded, all plants survived the experiment, even the one not receiving any water for 9 months. In a subsequent recovery experiment, the plants that were previously subjected to drought showed rapid recovery within 5 days. Additionally, the plant that received the highest amount of water was submerged in a bucket of water for three months and displayed no signs of stress; instead, its roots grew upward and out of the water (see Supplementary 1 online for details on the pretrial).
The effects of the invader in the main experiment were assessed by comparing the native species growing alone (intraspecific, i.e., the average of the two plants per pot) with those that were growing with the invader (interspecific) for the wet and dry treatment, respectively. The positions of the pots within the greenhouse were frequently interchanged to ensure similar environmental conditions and reduce edge effects of the glasshouse or general heterogeneity of environmental conditions. The plants were kept at an average of 40% humidity and in a 12-h day and night cycle, at temperatures of 20 °C and 12 °C, respectively.
Response parameters
We quantified above- and belowground net primary production at the end of the experiment (after 15 months of growth in competition), hereafter ANPP (Aboveground Net Primary Productivity) and BNPP (Belowground Net Primary Productivity). Belowground biomass was gently washed free from the substrate by rinsing it into a coarse sieve so that the substrate was washed away, and the roots and rootlets could all be collected. The above- and belowground biomasses were dried for five days at 60 °C and 100% ventilation and weighed.
Statistical analyses
The growth parameters (ANPP and BNPP) were analysed using a two-factorial analysis of variance (ANOVA66) with the explanatory factors being water regime (wet/dry) and competition (intraspecific/interspecific for both the native species and the invader, i.e., a factorial variable with four levels: native–native, native–invasive, invasive–native, and invasive–invasive, with the biomass value of the first named in each pair in interspecific competition and the average biomass of both individuals per pot in case of intraspecific competition) including their interaction. Single models were run for each native species (S. marginatum and R. communis) and each response parameter (ANPP and BNPP) for a total of four ANOVA analyses. Tukey’s HSD post hoc tests67 were used to assess the significance of differences in pairwise comparisons for significant interaction terms. Similarly, the same representation based on the total biomass production is also provided in the Supplementary 2 online.
Furthermore, two different competition indices were implemented; Logarithmic Response Ratio (lnRR) and Relative Neighbour Effect (RNE)3,68,69. As both indices yielded highly similar patterns, we present the results based on lnRR in the main text (Fig. 2) and based on RNE in the Supplementary 2 online.
The Logarithmic Response Ratio (lnRR) is computed by the natural log of the ratio between the mean value of the respective control treatment (intraspecific without a second species) and the value of each treatment growing in competition with a second species (interspecific). Smaller values indicate weaker competition with negative values showing facilitation, while larger values indicate intense competition between the species3,43. lnRR is expressed as:
where ln is the natural logarithm, \(P_{contr}\) is the performance of the plant growing in a monoculture and \(P_{mix}\) is the performance of a plant growing in a mixture.
The effect of the different water regimes on the competition index data was examined using a one-way analysis of variance with water regime (dry/wet) as explanatory factor. Single models were run for each native species (S. marginatum and R. communis) and each response parameter (ANPP and BNPP) for a total of four ANOVA analyses.
Parametric assumptions were checked for all ANOVA models by examining the diagnostic plots (residuals versus fitted plots for homoscedasticity of the residuals and normal qq-plots for normal distribution of residuals70. According to the diagnostic plots, the ANPP and BNPP for both the R. communis and the S. marginatum datasets were log(x + 1)-transformed. The competition index datasets did not require transformations. For graphical visualizations, the function bar graph.CI in the R package sciplot71 was used. All statistical analyses were done in R version 4.272.
Results
ANPP of O. ficus-indica was 4.3-fold higher under intraspecific competition than when competing with R. communis (Fig. 1a). In comparison, the native R. communis was 14-fold less productive aboveground than O. ficus-indica, but its ANPP was unaffected by the identity of its neighbour as its production did not differ between intra- and interspecific competition. O. ficus-indica was unaffected by the water regime in ANPP while the native R. communis produced 2.2 times more aboveground biomass under wet conditions and intraspecific competition than under dry conditions and interspecific competition (Fig. 1a).
BNPP of O. ficus-indica was 27-fold higher under intraspecific competition than when competing with R. communis (Fig. 1b). In comparison, the native R. communis was 17-fold less productive belowground under intraspecific competition than O. ficus-indica, but its BNPP even increased 3.1 times under interspecific as compared to intraspecific competition. O. ficus-indica doubled its BNPP under the dry as compared to the wet water regime when growing with itself but produced very little BNPP and showed no water effect when grown under interspecific competition. R. communis, in contrast, increased its BNPP in dry conditions 1.9 times over wet conditions only when growing in interspecific competition with O. ficus-indica, but not when growing with itself (Fig. 1b).
ANPP of O. ficus-indica was 3.8-fold higher under intraspecific competition than when competing with S. marginatum (Fig. 1c). In comparison, the native S. marginatum was 11-fold less productive aboveground than O. ficus-indica, but its ANPP was unaffected by the identity of its neighbour as its production did not differ between intra- and interspecific competition. In interspecific competition with S. marginatum, O. ficus-indica increased its ANPP 1.7-fold under wet as compared to dry conditions, while it did not show an aboveground growth response to the water regime when growing with itself. The ANPP of the native S. marginatum was unaffected by the water regime, irrespective of competition (Fig. 1c).
BNPP of O. ficus-indica was 2.8-fold higher under intraspecific competition than when competing with S. marginatum (Fig. 1d). In comparison, the native S. marginatum was only half as productive belowground as O. ficus-indica under intraspecific competition but showed a pattern similar to the latter as its BNPP was 3.0-fold higher when grown with itself than under interspecific competition. O. ficus-indica doubled its BNPP under the dry as compared to the wet water regime when growing with itself but produced less BNPP and showed no water effect when grown under interspecific competition. S. marginatum showed no significant effect in its BNPP on the water treatment (Fig. 1d).
Competition index
Ricinus communis and O. ficus-indica showed aboveground competition in their direct interaction, with the lnRR for ANPP being 3.4-fold higher for O. ficus-indica than for R. communis (1.51 ± 0.31 mean ± SD versus 0.45 ± 0.23, respectively; Fig. 2a). This pattern of aboveground competition was unaffected by the water regime. Belowground, the native R. communis was facilitated (− 1.00 ± 0.69, Fig. 2b) by O. ficus-indica which itself suffered strongly from high competition by the native species (3.35 ± 0.65; Fig. 2b). This pattern was significantly stronger for both species under dry than under wet conditions.
Solanum marginatum and O. ficus-indica showed aboveground competition in their direct interaction, which was 5.9-fold higher for O. ficus-indica than for S. marginatum (1.45 ± 0.44 versus 0.24 ± 0.33, respectively; Fig. 2c). This aboveground competition was 5.7-fold stronger for S. marginatum under wet as compared to dry conditions and 1.4-fold weaker for O. ficus-indica under wet as compared to dry conditions. Belowground, S. marginatum and O. ficus-indica showed about equal competition in their direct interaction (1.20 ± 0.46 versus 1.04 ± 0.52; Fig. 2d). For S. marginatum, belowground competition was unaffected by the water regime while competition increased 1.9-fold under dry as compared to wet conditions for O. ficus-indica.
Discussion
Opuntia ficus-indica, a highly successful invader of semiarid and arid ecosystems on all continents containing such conditions28, was an inferior competitor to Ricinus communis and Solanum marginatum, two species native to the highlands of Eritrea where O. ficus-indica is also spreading vigorously. These findings stem from our greenhouse pairwise competition experiment offering valuable insights to a possible explanation of potential outcomes in a field setting. O. ficus-indica dropped in ANPP and BNPP about fourfold and 3 to 27-fold, respectively, when growing together with R. communis and S. marginatum. Competition indices indicated high competition for O. ficus-indica irrespective of the competing native, while the natives experienced less competition or even facilitation by O. ficus-indica. These findings reject the hypothesis that the successful invader is a superior competitor to the native species73,74. The majority of pairwise competition trials between invasive and native species supported that hypothesis7. The notable exceptions in that meta-analysis stem mainly from temperate grasslands, which are known to harbour native species with high competitive abilities that can resist invasions75. Based on this and the competitive abilities found in our study, our invasive species should face strong resistance and have little success in invading native communities containing the two competitively superior native focal species. Surprisingly, that is not the case. O. ficus-indica spreads vigorously into areas containing those two native species5. So, the question arises: how can an inferior competitor be a successful invader?
The invasive O. ficus-indica produced several times more biomass than the natives, even under competition with the natives where it is doing much worse than on its own. Therefore, it ‘outproduces’ the competitively stronger natives in all cases except for BNPP with R. communis. Although we didn't quantify belowground biomass at transplantation to keep root systems intact, visual assessment revealed no significant differences among the three plant species. Thus, to better understand the role of competition in plant invasion, the relative biomass production of an invasive species compared to the native species should therefore be taken into consideration. Based on a meta-analysis of interspecific and intraspecific competition trials, Vilà and Weiner7 found that mixtures of native and invasive species are less productive than native monocultures but not less productive than monocultures of the invasive plants. In our study, the interspecific mixtures produced, on average, 13.9 g aboveground biomass per pot, which is more than twice the production of the native species in monoculture (5.7 g) but nearly seven times less than the invasive species in monoculture (97.1 g). The numbers for BNPP followed a similar pattern, being 2.9 g for the mixture, 3.3 g for the native monocultures, and 14.1 g for the invasive monocultures, respectively. Comparing these numbers to the meta-analysis implies that our finding of a highly productive but weakly competitive invasive species might be an interesting exception rather than the rule.
The results of our experiment showed that competition by the native species generally increased for O. ficus-indica under the dry as compared to the wet treatment (Fig. 2). At first sight, this finding contradicts our second hypothesis which expected higher competitive power of O. ficus-indica under dry conditions. However, this weak competition should not be interpreted as a sign of reduced invasion pressure on the community. O. ficus-indica is a plant that has adapted to endure extreme environmental conditions and flourish in arid environments. By utilizing Crassulacean Acid Metabolism (CAM) photosynthesis, O. ficus-indica strategically minimizes moisture loss and enhances water-use efficiency, making it a highly drought-tolerant species that can cause pressure on the native community76. Moreover, the unique ability of O. ficus-indica's fallen cladodes to undergo vegetative growth is a fundamental trait that contributes to its spread. This growth strategy enables O. ficus-indica to outgrow native species and establish a significant presence.
We based the water regimes of our experiment on pretrials that tested the water tolerance limits of the target species and found increased mortality of the native species right below our dry water regime. The ability of O. ficus-indica to survive well below this limit probably contributes greatly to its invasion success in dry ecosystems. Moreover, within the dry ecosystems, water availability becomes a major source of competition, favouring plants like O. ficus-indica which are capable of enduring the water shortages19, but readily taking up large amounts of water as soon as it becomes available with its rapidly forming rain roots and storage in its succulent tissue77. Our pretrial showed that it even survived nine months without any water addition in our experimental setup (see Supplementary 1 online). This extreme drought tolerance is an important advantage for O. ficus-indica, as drought is frequent in the highlands of Eritrea78. The native species are expected to be adapted to what was the norm frequency and magnitude of drought, but climate change will result in stronger and more frequent drought events39, which will challenge their survival. Disturbance in the native vegetation due to drought might therefore create gaps with little to no interspecific competition in which the invasive species can spread even more rapidly17. Notably, O. ficus-indica was also able to survive very wet periods according to our pretrials. Even complete submergence of the pots for three months did not kill the plants but resulted in roots growing on the surface of the water, probably seeking oxygen access (see Supplementary 1 online). Besides ‘outproducing’ native species by high biomass production, these extreme tolerance limits are another potential explanation for the global invasion success of O. ficus-indica.
The two native species showed interesting differences in their response to competition by O. ficus-indica. R. communis increased its BNPP under interspecific competition with O. ficus-indica when stressed with water shortage (dry water regime). This is reflected in the competition index indicating facilitation for R. communis by O. ficus-indica (Fig. 2). Fighting against O. ficus-indica belowground, however, did not help its general case as it was still ‘outproduced’ by O. ficus-indica aboveground. The ANPP of O. ficus-indica in interspecific competition with R. communis is quite remarkable when taking the very low BNPP due to high interspecific competition into consideration. S. marginatum, on the other hand, appeared to give in to the interspecific competition by O. ficus-indica belowground, as it showed decreased BNPP compared to the intraspecific interaction. O. ficus-indica is able to produce about 10 times as much root biomass in the presence of S. marginatum than in the presence of R. communis. Still, S. marginatum was successful in competing with O. ficus-indica aboveground, where it showed low competition, especially under dry conditions. Despite the efforts to compete with O. ficus-indica aboveground, it did not alter the overall outcome, as it was still ‘outproduced’ by O. ficus-indica aboveground. Potential explanations for the different behaviour of the native species in response to competition by O. ficus-indica might be that S. marginatum unlike R. communis is rather a drought-tolerant species that can better survive longer periods without water62. In any case, neither strategy appeared to be successful as both natives are clearly outperformed by O. ficus-indica in ANPP, irrespective of the water regime and despite them being superior competitors to O. ficus-indica.
Conclusion
A species successfully invading semiarid to arid ecosystems across the globe, O. ficus-indica, is an inferior competitor to two common and widespread native species of the highlands of Eritrea. One of the natives, R. communis, successfully competed with O. ficus-indica belowground, and the other native, S. marginatum, showed superior competition mainly aboveground. This finding contradicts the common implicit interpretation that successful invasive species have overall advantageous traits that make them stronger competitors than native species. Being vastly more productive, even under interspecific competition, and considerably more tolerant against water stress than the native species appears more important than competitive power, at least for invasions in semiarid to arid, open vegetation. Stronger and more frequent disturbance of the native vegetation by drought due to climate change will further accelerate the success of O. ficus-indica, as it is extremely drought tolerant and produces considerably more biomass in the absence of interspecific competition.
Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author upon reasonable request.
References
Gioria, M. & Osborne, B. A. Resource competition in plant invasions: Emerging patterns and research needs. Front. Plant Sci. 5, 501. https://doi.org/10.3389/fpls.2014.00501 (2014).
Parker, I. M. & Reichard, S. H. Critical issues in invasion biology for conservation science. In Conservation Biology (eds Fiedler, P. L. & Kareiva, P. M.) 283–305 (Springer, 1998).
Weigelt, A. & Jolliffe, P. Indices of plant competition. J. Ecol. 91, 707–720. https://doi.org/10.1046/j.1365-2745.2003.00805.x (2003).
Maron, J. L. & Marler, M. Field-based competitive impacts between invaders and natives at varying resource supply. J. Ecol. 96, 1187–1197. https://doi.org/10.1111/j.1365-2745.2008.01440.x (2008).
Tesfay, Y. B. & Kreyling, J. The invasive Opuntia ficus-indica homogenizes native plant species compositions in the highlands of Eritrea. Biol. Invasions 23, 433–442. https://doi.org/10.1007/s10530-020-02373-8 (2021).
Vilà, M., Williamson, M. & Lonsdale, M. Competition experiments on alien weeds with crops: Lessons for measuring plant invasion impact?. Biol. Invasions 6, 59–69. https://doi.org/10.1023/b:binv.0000010122.77024.8a (2004).
Vilà, M. & Weiner, J. Are invasive plant species better competitors than native plant species? Evidence from pair-wise experiments. Oikos 105, 229–238. https://doi.org/10.1111/j.0030-1299.2004.12682.x (2004).
Pyšek, P. & Richardson, D. M. Invasive species, environmental change and management, and health. Annu. Rev. Environ. Resour. 35, 25–55. https://doi.org/10.1146/annurev-environ-033009-095548 (2010).
Roy, J. In search of the characteristics of plant invaders. In Biological Invasions in Europe and the Mediterranean Basin 335–352 (Springer, 1990).
Wilson, J. R. U. et al. Residence time and potential range: Crucial considerations in modelling plant invasions. Divers. Distrib. 13, 11–22. https://doi.org/10.1111/j.1366-9516.2006.00302.x (2007).
MacDougall, A. S. & Turkington, R. Are invasive species the drivers or passengers of change in degraded ecosystems?. Ecology 86, 42–55. https://doi.org/10.1890/04-0669 (2005).
Levine, J. M. et al. Mechanisms underlying the impacts of exotic plant invasions. Proc. Biol. Sci. 270, 775–781. https://doi.org/10.1098/rspb.2003.2327 (2003).
Lonsdale, W. M. & Farrell, G. S. Testing the effects on Mimosa pigra of a biological control agent Neurostrota gunniella (Lepidoptera: Gracillaridae), plant competition and fungi under field conditions. Biocontrol Sci. Technol. 8, 485–500. https://doi.org/10.1080/09583159830009 (1998).
Willis, A. J., Groves, R. H. & Ash, J. E. Interactions between plant competition and herbivory on the growth of hypericum species: A comparison of glasshouse and field results. Aust. J. Bot. 46, 707. https://doi.org/10.1071/BT97025 (1998).
Müller-Schärer, H. The impact of root herbivory as a function of plant density and competition: Survival, growth and fecundity of Centaurea maculosa in field plots. J. Appl. Ecol. 28, 759. https://doi.org/10.2307/2404206 (1991).
Davis, M. A., Grime, J. P. & Thompson, K. Fluctuating resources in plant communities: A general theory of invasibility. J. Ecol. 88, 528–534. https://doi.org/10.1046/j.1365-2745.2000.00473.x (2000).
Hughes, A. R., Byrnes, J. E., Kimbro, D. L. & Stachowicz, J. J. Reciprocal relationships and potential feedbacks between biodiversity and disturbance. Ecol. Lett. 10, 849–864. https://doi.org/10.1111/j.1461-0248.2007.01075.x (2007).
Yin, W. et al. Rapid evolutionary trade-offs between resistance to herbivory and tolerance to abiotic stress in an invasive plant. Ecol. Lett. 26, 942–954. https://doi.org/10.1111/ele.14221 (2023).
Craine, J. M. & Dybzinski, R. Mechanisms of plant competition for nutrients, water and light. Funct. Ecol. 27, 833–840. https://doi.org/10.1111/1365-2435.12081 (2013).
Foxx, A. J. & Fort, F. Root and shoot competition lead to contrasting competitive outcomes under water stress: A systematic review and meta-analysis. PLoS One 14, e0220674. https://doi.org/10.1371/journal.pone.0220674 (2019).
Chen, J.-J. et al. Effects of water availability on leaf trichome density and plant growth and development of Shepherdia xutahensis. Front. Plant Sci. 13, 855858. https://doi.org/10.3389/fpls.2022.855858 (2022).
Brendel, O. The relationship between plant growth and water consumption: a history from the classical four elements to modern stable isotopes. Ann. For. Sci. https://doi.org/10.1007/s13595-021-01063-2 (2021).
Nobel, P. S. Cacti. In Biology and Uses (ed. Nobel, P. S.) (University of California Press, 2002).
Masrahi, Y., Al-Namazi, A., Alammari, B. & Alturki, T. Adaptations facilitate the invasion of Cylindropuntia rosea (DC.) Backeb. (Cactaceae) in the highlands of southwestern Saudi Arabia. Plant Signal. Behav. 17, 2144593. https://doi.org/10.1080/15592324.2022.2144593 (2022).
Brutsch, M. O. & Zimmermann, H. G. Control and utilization of wild opuntias. FAO Plant Prod. Prot. Pap. 132, 155–166 (1995).
Obiri, J. F. Invasive plant species and their disaster-effects in dry tropical forests and rangelands of Kenya and Tanzania. Jàmbá J. Disaster Risk Stud. https://doi.org/10.4102/jamba.v3i2.39 (2011).
Oduor, A. M. O., Long, H., Fandohan, A. B., Liu, J. & Yu, X. An invasive plant provides refuge to native plant species in an intensely grazed ecosystem. Biol. Invasions 20, 2745–2751. https://doi.org/10.1007/s10530-018-1757-5 (2018).
Novoa, A., Le Roux, J. J., Robertson, M. P., Wilson, J. R. U. & Richardson, D. M. Introduced and invasive cactus species: A global review. AoB Plants https://doi.org/10.1093/aobpla/plu078 (2015).
Jones, C. G., Lawton, J. H. & Shachak, M. Organisms as ecosystem engineers. Oikos 69, 373. https://doi.org/10.2307/3545850 (1994).
Shackleton, R. T., Witt, A. B. R., Piroris, F. M. & van Wilgen, B. W. Distribution and socio-ecological impacts of the invasive alien cactus Opuntia stricta in eastern Africa. Biol. Invasions 19, 2427–2441. https://doi.org/10.1007/s10530-017-1453-x (2017).
Hussein, A. & Estifanos, S. Modeling impacts of climate change on the distribution of invasive Opuntia ficus-indica (L.) Mill. in Ethiopia: Implications on biodiversity conservation. Heliyon 9, e14927. https://doi.org/10.1016/j.heliyon.2023.e14927 (2023).
Sipango, N. et al. Prickly pear (Opuntia spp.) as an invasive species and a potential fodder resource for ruminant animals. Sustainability 14, 3719. https://doi.org/10.3390/su14073719 (2022).
Abay, N. G. Cactus (Opuntia ficus-indica): Current utilization and future threats as cattle forage in Raya-Azebo, Ethiopia. EMSD 7, 1. https://doi.org/10.5296/emsd.v7i3.12806 (2018).
Snyman, H. A. Growth rate and water-use efficiency of cactus pears Opuntia ficus-indica and O. robusta. Arid Land Res. Manag. 27, 337–348. https://doi.org/10.1080/15324982.2013.771232 (2013).
Fonseca, V. A. et al. Morpho-physiology, yield, and water-use efficiency of Opuntia ficus-indica irrigated with saline water. Acta Sci. Agron. 41, 42631. https://doi.org/10.4025/actasciagron.v41i1.42631 (2018).
Shackleton, C. M. et al. Assessing the effects of invasive alien species on rural livelihoods: Case examples and a framework from South Africa. Hum. Ecol. 35, 113–127. https://doi.org/10.1007/s10745-006-9095-0 (2007).
Luo, Y. & Nobel, P. S. Growth characteristics of newly initiated cladodes of Opuntia ficusindica as affected by shading, drought and elevated CO2. Physiol. Plant 87, 467–474. https://doi.org/10.1111/j.1399-3054.1993.tb02495.x (1993).
Ali, N., Mounir, L. & Hichem, B. S. Cactus as a tool to mitigate drought and to combat desertification. J. Arid Land Stud. 24, 121–124 (2014).
IPCC Climate change 2022: Impacts, adaptation, and vulnerability. In Contribution of Working Group II to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change (eds Pörtner, H.-O. et al.) (Cambridge University Press, 2022).
Goldberg, D. E. & Werner, P. A. Equivalence of competitors in plant communities: A null hypothesis and a field experimental approach. Am. J. Bot. 70, 1098–1104. https://doi.org/10.1002/j.1537-2197.1983.tb07912.x (1983).
Grace, J. B. On the measurement of plant competition intensity. Ecology 76, 305–308. https://doi.org/10.2307/1940651 (1995).
Gurevitch, J., Morrow, L. L., Wallace, A. & Walsh, J. S. A meta-analysis of competition in field experiments. Am. Nat. 140, 539–572. https://doi.org/10.1086/285428 (1992).
Goldberg, D. E., Rajaniemi, T., Gurevitch, J. & Stewart-Oaten, A. Equivalence of competitors in plant communities: A null hypothesis and a field experimental approach. Ecology 80, 1118–1131. https://doi.org/10.1890/0012-9658(1999)080[1118:EATQII]2.0.CO;2 (1999).
Adler, P. B. et al. Competition and coexistence in plant communities: Intraspecific competition is stronger than interspecific competition. Ecol. Lett. 21, 1319–1329. https://doi.org/10.1111/ele.13098 (2018).
Chesson, P. Mechanisms of maintenance of species diversity. Annu. Rev. Ecol. Syst. 31, 343–366. https://doi.org/10.1146/annurev.ecolsys.31.1.343 (2000).
Ogbazghi, W. & Stillhardt, B. Sustainable Land Management—A textbook with a focus on Eritrea (Bern, Geographica Bernensia and Hamelmalo Agricultural College, Keren, 2011).
Hedberg, I. & Edwards, S. Flora of Ethiopia and Eritrea. Pittosporaceae to Araliaceae Vol. 3 (National Herbarium, 1989).
Edwards, S., Mesfin, T. & Hedberg, I. Flora of Ethiopia and Eritrea. Canellaceae to Euphorbiaceae (Addis Abeba University, 1995).
Edwards, S., Sebsebe, D. & Hedberg, I. Flora of Ethiopia and Eritrea, Hydrocharitaceae to Arecaceae (Addis Abeba University, 1997).
Edwards, S., Mesfin, T., Sebsebe, D. & Hedberg, I. Flora of Ethiopia and Eritrea, Magnoliaceae to Flacourtiaceae (Addis Abeba University, 2000).
Hedberg, I., Edwards, S. & Sileshi, N. Flora of Ethiopia and Eritrea, Apiaceae to Dipsacaceae (Addis Abeba University, 2003).
Hedberg, I., Ensermu, K., Edwards, S., Sebsebe, D. & Persson, E. Flora of Ethiopia and Eritrea, Gentianaceae to Cyclocheilaceae (Addis Abeba University, 2006).
Mesfin, T. Flora of Ethiopia and Eritrea, Asteraceae (Compositae) (Addis Abeba University, 2004).
Bein, E., Habte, B., Jaber, A., Birnie, A. & Tengnas, B. Useful Trees and Shrubs in Eritrea (Regional Soil Conservation Unit (RSCU), 1996).
Gimeno, I. & Vilà, M. Recruitment of two Opuntia species invading abandoned olive groves. Acta Oecologica 23, 239–246. https://doi.org/10.1016/S1146-609X(02)01143-8 (2002).
Nieddu, G. & Chessa, I. Distribution of phenotypic characters within a seedling population from Opuntia ficus-indica. Acta Hortic. https://doi.org/10.17660/ActaHortic.1997.438.4 (1997).
Altare, M., Trione, S., Guevara, J. & Cony, M. Stimulation and promotion of germination in Opuntia ficus-indica seeds. J. Prof. Assoc. Cactus Dev. 8, 91–100 (2016).
Gordon, D. R., Tancig, K. J., Onderdonk, D. A. & Gantz, C. A. Assessing the invasive potential of biofuel species proposed for Florida and the United States using the Australian Weed Risk Assessment. Biomass Bioenergy 35, 74–79. https://doi.org/10.1016/j.biombioe.2010.08.029 (2011).
Zarai, Z. et al. Essential oil of the leaves of Ricinus communis L.: In vitro cytotoxicity and antimicrobial properties. Lipids Health Dis. 11, 102. https://doi.org/10.1186/1476-511X-11-102 (2012).
Hedberg, I. (ed.). Flora of Ethiopia (National Herbarium [etc.], Addis Ababa [etc.], 2006).
Fichtl, R. & Adi, A. Honeybee Flora of Ethiopia (Margraf, 1994).
Asefa, A. & Gashe, G. Role of native herbivores in the increasing abundance of Solanum marginatum L.F. (Solanaceae) in the northern Bale mountains, Ethiopia. SINET Ethiop. J. Sci. 40, 74–87 (2017).
Williams, S. Bale Mountains: A Guide Book (Ethiopian Wolf Conservation Programme, 2002).
Abebe, D., Debella, A. & Urga, K. Illustrated Checklist, Medicinal Plants and Other Useful Plants of Ethiopia (Ethiopian Health and Nutrition Research Institute, 2003).
Aynekulu, E. et al. Plant diversity and regeneration in a disturbed isolated dry Afromontane forest in northern Ethiopia. Folia Geobot. 51, 115–127. https://doi.org/10.1007/s12224-016-9247-y (2016).
Kuznetsova, A., Brockhoff, P. B. & Christensen, R. H. B. lmerTest package: Tests in linear mixed effects models. J. Stat. Soft. https://doi.org/10.18637/jss.v082.i13 (2017).
Lenth, R. _emmeans: Estimated Marginal Means, aka Least-Squares Means. https://CRAN.R-project.org/package=emmeans (2022).
Oksanen, L., Sammul, M. & Mägi, M. On the indices of plant–plant competition and their pitfalls. Oikos 112, 149–155. https://doi.org/10.1111/j.0030-1299.2006.13379.x (2006).
Markham, J. H. & Chanway, C. P. Measuring plant neighbour effects. Funct. Ecol. 10, 548–549 (1996).
Faraway, J. J. Linear Models with R (Chapman and Hall/CRC, 2004).
Morales, M. sciplot: Scientific Graphing Functions for Factorial Designs https://CRAN.R-project.org/package=sciplot (2020).
R Core Team. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/ (2022).
D’Antonio, C. Impacts and extent of biotic invasions in terrestrial ecosystems. Trends Ecol. Evol. 17, 202–204. https://doi.org/10.1016/s0169-5347(02)02454-0 (2002).
Zheng, Y. et al. Are invasive plants more competitive than native conspecifics? Patterns vary with competitors. Sci. Rep. 5, 15622. https://doi.org/10.1038/srep15622 (2015).
Thomsen, M. A. & D’Antonio, C. M. Mechanisms of resistance to invasion in a California grassland: The roles of competitor identity, resource availability, and environmental gradients. Oikos 116, 17–30. https://doi.org/10.1111/j.2006.0030-1299.14929.x (2007).
de La Barrera, E. & Nobel, P. S. Carbon and water relations for developing fruits of Opuntia ficus-indica (L.) Miller, including effects of drought and gibberellic acid. J. Exp. Bot. 55, 719–729. https://doi.org/10.1093/jxb/erh084 (2004).
Snyman, H. A. Root studies on cactus pears Opuntia ficus-indica and O. robusta along a soil–water gradient. Haseltonia 13, 64–75. https://doi.org/10.2985/1070-0048(2007)13[64:RSOCPO]2.0.CO;2 (2007).
Funk, C. et al. The centennial trends greater horn of Africa precipitation dataset. Sci. Data 2, 150050. https://doi.org/10.1038/sdata.2015.50 (2015).
Acknowledgements
We thank Ilka Beil and Andrey Malyshev for their helpful input and greenhouse assistance. Yohannes B. Tesfay was supported by the DFG research training group RESPONSE (RTG 2010) and a scholarship from the German Academic Exchange Service (DAAD).
Funding
Open Access funding enabled and organized by Projekt DEAL. YT was supported by the DFG research training group RESPONSE (RTG 2010) and a scholarship from the German Academic Exchange Service (DAAD).
Author information
Authors and Affiliations
Contributions
Y.T. and J.K. contributed to the study conception and design. Material preparation, data collection and analysis were performed by Y.T. The pretrial experiment, design, data collection and analysis were performed by A.B. The first draft of the manuscript was written by Y.T. and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Tesfay, Y.B., Blaschke, A. & Kreyling, J. An inferior competitor is a successful invader due to its stress tolerance and productivity. Sci Rep 13, 20694 (2023). https://doi.org/10.1038/s41598-023-48152-y
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-023-48152-y
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.