Abstract
The screening of siRNAs targeting 390 human G protein-coupled receptors (GPCRs) was multiplexed in combination with cisplatin against lung cancer cells. While the cell viability measure hardly captured the anticancer effect of siGPCRs, the direct cell count revealed the anticancer potential of diverse GPCRs (46 hits with > twofold growth inhibition, p-value < 0.01). In combined treatment with cisplatin, siRNAs against five genes (ADRA2A, F2RL3, NPSR1, NPY and TACR3) enhanced the anti-proliferation efficacy on cancer cells and reduced the self-recovery ability of surviving cells after the removal of the combined treatment. Further on-target validation confirmed that the knockdown of TACR3 expression exhibited anticancer efficacy under both single and combined treatment with cisplatin. Q-omics (http://qomics.io) analysis showed that high expression of TACR3 was unfavorable for patient survival, particularly with mutations in GPCR signaling pathways. The present screening data provide a useful resource for GPCR targets and biomarkers for improving the efficacy of cisplatin treatment.
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Introduction
G protein-coupled receptors (GPCRs) are major targets of therapeutic drugs against diverse diseases1. Their roles and functions have been recently reported in the cancer context, providing new strategies for cancer drug development. Several drugs or drug candidates targeting GPCRs are already in clinical use or in trials. For example, there are a few FDA-approved cancer drugs available, such as cabergoline, lanreotide, degarelix, plerixafor, vismodegib and raloxifene, that target DRD1 (dopamine receptor D1), SSTR (somatostatin receptor), GnRH (gonadotropin-releasing hormone receptor), CXCR4 (C-X-C chemokine receptor type 4), SMO (smoothened homolog) and ES (estrogen receptor)2,3. More compounds targeting GPCRs, such as CCR2/CCR4 (C–C chemokine receptor type 2/4), FZD4 (frizzled class receptor 4), and PE2/PE4 (prostaglandin receptors), are under clinical trials4,5,6. However, GPCRs have not been widely investigated as cancer targets, partly because of their low inhibitory outcome from conventional in vitro cytotoxicity (i.e., cell viability) assays6.
Cell viability measures based on mitochondrial activity have been widely used as surrogates for the mass change of cancer cells in screens. However, recent studies showed that cell viability measures from surviving cells also represent the resistant phenotype overcoming the effect of anticancer treatments; thus, the measure is not directly correlated with the cell number, particularly in the screen with the treatment of stress-inducing apoptotic anticancer agents7,8,9. In our previous study, we observed negative correlations between the total cell count and the viability of surviving single cells after treatment with anti-proliferative short interfering RNAs (siRNAs)9. However, the image-based direct cell count provided greater resolution than conventional viability measures for the quantification of the anticancer efficacy of siRNAs.
Here, we carried out multiplexed screening of siGPCRs by using simultaneous measures of cell viability (mitochondrial activity) and image-based cell counts (Fig. 1). This multiplexing might effectively dissect the sensitive and resistant responses of cells against anticancer treatment, revealing further clues on the self-renewal ability of surviving cancer cells. Significant amounts of data have accumulated from forward screening campaigns using pooled short hairpin RNA (shRNA) or single-guide RNA (sgRNA) libraries against diverse cancer cell lines10,11. Data from well-based reverse screening using a siRNA library have limited availability from public domains but have advantages in compatibility with drug screen data and in employing diverse (or multiplexed) phenotypic assays9,12,13.
Cisplatin is a first-line chemotherapeutic against lung cancers and many other cancer types7,8,14,15. Although many combination targets for cisplatin have been reported, GPCRs have not been systematically investigated as potential targets for cisplatin combination therapy or for overcoming cisplatin resistance in cancers. Together with a single treatment of siGPCRs, we comparatively screened the combined treatment of siGPCRs with cisplatin against lung cancer cells. We expect that the present multiplexed screening provides sufficient resolution to capture the additive or synergistic effect of siGPCRs on cisplatin treatment.
Results
Data production from multiplexed screening of siGPCRs
A total of 390 siGPCRs were screened in both single and combined treatment with cisplatin against the A549 cell line (Supplementary Fig. S1). In this study, we carried out each of three screening repeats as a separate batch using independently cultured cells, thus improving the reproducibility of the outcome. The measure of the anticancer effect was multiplexed by adapting the cell viability assay together with simultaneous direct cell counting (see “Methods” for details). The z’-factor provides a quantitative measure of the data quality of the whole screening by measuring the resolution between positive and negative controls in the screening16 (Supplementary Fig. S2). Overall, the readout (Hoechst) of cell counting exhibited greater z’-factors than the cell viability measure (TiterBlue) in this screening. Most Z’ factors from the cell counting were above 0.5, while many Z’ factors from the cell viability assay did not reach 0.5. Consistent with the finding of our previous screening study12, this result shows that image-based cell counting provides greater resolution in the measure than the fluorescence-based cell viability assay, although the cell count measure has limited application to cells with minimal self-aggregation propensity in the course of proliferation. The complete list of screening results are available in Supplementary Table S1.
Anticancer efficacy of siGPCRs in single and combined treatment with cisplatin
The anticancer efficacy of 390 siGPCRs in the single treatment exhibited a wider distribution in the cell count assays than in the cell viability assay (Fig. 2A), although there was a general correlation in the results between the two assays. siGPCR hits with significant (p-value < 0.01) efficacy were found to have up to a fourfold (log2-fold) difference from siNC in the cell count assay, while the difference was up to twofold in the cell viability assay. The result of siGPCR screening in combination with cisplatin treatment also showed that the cell count provided a wider distribution of efficacy than the cell viability assay (Fig. 2B).
From the three repeats of screening, we identified a total of 46 siGPCRs as significant (p-value < 0.01) hits from cell count assays (blue and red dots in Fig. 2C). The complete list of hit genes is available in Table 1. Most of them showed prognostic RNA expression in LUAD patient samples (prognostic hit genes are colored with blue and red in Table 1). Their expression was significantly associated with patient survival in the Kaplan–Meier plot analysis. The common 37 siGPCR hits showed consistent cell growth inhibition (i.e., > twofold change) in both single and combined treatment with cisplatin (red dots in Fig. 2C). Furthermore, we identified nine siGPCRs exhibiting a significant (p-value < 0.01) increase in inhibition when combined with cisplatin (blue dots in Fig. 2C). However, most of the cell count hits were not recognized in the cell viability assay (Fig. 2D). Only a few hits were identified from the viability measure, and most siGPCRs showed nonsignificant outcomes.
From the simultaneous multiplexed assays, we also measured the “viability per cell”, which was derived by dividing the well-based viability score by the cell count (i.e., the total number of cells in the well) (Fig. 2E). The results showed that the viability per cell generally increased as the cell count decreased. This implies that surviving cells from anti-proliferative treatments generally have higher mitochondrial activity (i.e., viability) than cells under normal conditions. siGPCR hits that significantly decreased the cell count exhibited a diverse range of viability per cell in surviving cells. Interestingly, the positive control, siPLK1 treatment, reduced the cell number without increasing the viability per cell in surviving cells.
Validation of siGPCR hits for synergy with cisplatin treatment
Among 46 siGPCR hits, we selected seven siGPCRs (siADRA2A, siEMR3, siF2RL3, siGPC108, siNPSR1, siNPY and siTACR3) for further validation (Fig. 3A). Their anticancer effects in single and combined treatment with cisplatin were measured by cell counting. Consistent with the primary screening result shown in Fig. 2C, they showed a marginal (but significant) anti-proliferation effect in the single treatment, except that siTACR3 failed to reproduce the significant effect. When combined with cisplatin, five of them, except siEMR3 and siGPR108, reproduced the significant enhancement in the anti-proliferation effect of the combined treatment in comparison to cisplatin treatment alone.
We also analyzed the self-renewal ability of surviving cells after siGPCR and/or cisplatin treatments (Fig. 3B). Surviving cells were transferred to fresh media, and their proliferation ability was measured by cell counting. We observed that all five siGPCRs (siEMR3, siF2RL3, siGPR108, siNPSR1 and siTACR3) markedly decreased the self-renewal ability of cancer cells when they were treated with cisplatin. Although they induced minimal change in the self-renewal ability by single treatment, they showed synergistic effects when combined with cisplatin (Supplementary Table S2 and Fig. 3B). The cell count decrease from the combination of siRNAs with Cisplatin was greater than the sum of decreased cell counts of single treatments of siRNA and Cisplatin (i.e., Red additive line in the Fig. 3B).
To evaluate the off-target contribution to the anti-proliferation effect of siGPCRs, we carried out the knockdown experiment again with the increased number of diversified siRNA probe sequences per gene. For the primary screening, we used a pool of five siRNA probes per gene and treated them at a final concentration of 10 nM. Here, a total of 20 different probe sequences were pooled against a given target gene, and a 2.5-fold lower final concentration (4 nM) of the treatment was used for the second validation (Fig. 4). As a result, their marginal anti-proliferation effect in the single treatment was consistently reproduced (Fig. 4A). siTACR3 also showed significant efficacy. Synergistic inhibition with the cisplatin combination was only significantly observed with siTACR3 treatment. The combination of the other four siGPCRs with cisplatin showed similar efficacy to cisplatin single treatment. We also investigated the anti-proliferation effect of the serial treatment of cisplatin and newly designed siGPCRs (Fig. 4B). Surviving cells from pretreated cisplatin were compared with fresh cells under siGPCR treatment. All five siGPCRs showed greater anti-proliferation effects on cisplatin-pretreated cells than on fresh cells. In these secondary validations with newly designed siRNAs (Figs. 4A and 2D), siTACR3 exhibited a greater inhibitory effect than the other siGPCRs in both single and combined treatment with cisplatin.
Prognostic RNA expression of TACR3 and associated mutations in patient samples
We investigated whether the RNA expression of TACR3 was associated with the survival rate of LUAD patients. In the extensive analysis of 490 patient samples derived from TCGA (https://portal.gdc.cancer.gov/) using Q-omics software (http://qomics.io)17,18, TACR3 expression was not associated with patient survival in any sample filtering conditions (OS/DFS, Median/Quartile, Stage-I/II/III/IV, Male/Female, Diverse subtypes, etc.) (Data not shown, see “Methods” for details). However, Q-omics software showed that overexpression of TACR3 was significantly associated with the survival rate in patient samples harboring one or more of 15 mutations (Supplementary Table S3). These mutations were found in GCPR signaling, immune response and transport functions. Under one or more mutations in these genes, TACR3 overexpression was unfavorable for LUAD patient survival (Fig. 5A). These mutations were found in 109 patient samples and were not specifically associated with other major oncogenic mutations (Fig. 5B). Together with the significant anti-proliferation effect of TACR3 knockdown (Fig. 3), the prognostic overexpression of TACR3 in patients harboring given mutations provides promising target-biomarker pairs for improved anticancer therapies.
Discussion
The anti-proliferation potential of GPCRs has not been widely recognized in screening campaigns against cancers. Anticancer screening typically adapts cell viability assays measuring mitochondrial activity or ATP levels in live cells after treatment19,20,21. These viability measures are assumed to be correlated with the mass change of cancer cells in the screen, thus representing the anticancer efficacy of the treated agent. However, cell viability measures are not directly correlated with the change in cell count due to the increased mitochondrial activity in surviving cells after anticancer treatment9. Thus, when the image-based direct cell count was multiplexed with a cell viability assay, it provided improved resolution in identifying the anticancer potential of treatments9. The present screenings also showed that the cell viability measure provided poor resolution in quantifying the anticancer effect of 390 siGPCRs (Fig. 2). Over 10% of GPCRs exhibited significant anticancer potential from the direct cell count assay, while the cell viability measure showed only a few significant hits.
The viability per cell measure (Fig. 2E) might represent the resistance potential in surviving cells. A previous study showed that the viability per cell measure was correlated with the self-renewal ability of surviving cells after anticancer siRNA treatment9. We will further investigate whether this measure is correlated with the self-renewal ability of cells after the combined treatment of siGPCRs and cisplatin.
In the present screening, most of the primary hits were successfully reproduced in the validation steps (Fig. 3). We carried out three independent batches of screening using cells from different passages. The treatment concentration of siRNA was minimal at 10 nM and 4 nM for the Dharmacon and siPools siRNAs, respectively. We believe that the low concentration pooling of diverse siRNA probes (i.e., 20 probes per gene from siPools) effectively compromised the sensitivity and specificity against the target gene, thus minimizing the off-target effect.
Although TACR3 plays significant roles in physiological development and specifically in the human reproductive system, its role in carcinoma is unknown22. The present study showed that TACR3 might be a noble anticancer target, particularly with combined or serial treatment with cisplatin, one of the first-line drugs. The expression level of TACR3 was low in the normal epithelium and was highly elevated in tumor cells22. Interestingly, TACR3 overexpression was significantly associated with a poor survival rate when combined with mutations in other GPCR genes (Fig. 5). Although GPCR somatic mutations are widely present in LUAD samples, they have not yet provided therapeutic strategies. The present observation provides new insights into targeting GPCRs with GPCR mutations as patient stratification biomarkers.
Methods
Cell culture conditions
The non-small-cell lung cancer cell lines used in experiments included A549, EKVX (National Institutes of Health, National Cancer Institute, Frederick, MD, USA), H1793, and H1437(Korean Cell Line Bank, Korean Cell Line Research Foundation, Seoul, Korea). The cell lines were maintained in RPMI 1640 medium containing 10% fetal bovine serum (HyClone Laboratories, Logan, UT, USA) and 1% penicillin. For H1437, RPMI 1640, HEPES (GIBCO Laboratories, Grand Island, New York) was used. The cells were cultured in a humidified atmosphere of 5% CO2 and 95% air at 37 °C, and the culture medium was refreshed every two to three days.
High-throughput siRNA screening
The siRNA screen was performed by using four pooled On-Target Plus siRNAs to target each of the 390 genes in the human GPCR siRNA library. Screening was performed in triplicate for four days in a 384-well plate format (400 cells per well). Each plate was supplied with a negative control siRNA (siNC; GE Dharmacon) and positive control siRNA (PLK1; GE Dharmacon) to distinguish sequence-specific silencing from nonspecific efficiency and the effects of knockdown. Reverse transfection was performed using a MultiFlo microplate dispenser (BioTek Instruments) with siRNAs (final concentration of 10 nM) and Lipofectamine RNAiMAX (Thermo Fisher Scientific, Waltham, MA, USA; 0.05 µl per well) diluted Opti-MEM (Thermo Fisher Scientific; 15 µl per well) in a black 384-well plate (Corning, Corning, NY, USA). After 24 h, each set was treated with 0.1% dimethylformamide (DMF) and cisplatin (final concentration of 1 µM). Cisplatin was obtained from Abmole Bioscience and dissolved in 100% DMF at a final concentration of 50 µM. Aliquots were stored at -20 °C and thawed and diluted (1 µM) immediately before use. After 72 h, the cells were assayed using the CellTiter-Blue Cell Viability Assay Kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol, and the fluorescence produced was proportional to the number of viable cells. Plates were read on a SYNERGY H1 microplate reader (BioTek Instruments). After the cell viability assay, the cells were stained with Hoechst 33342 (Sigma–Aldrich, St. Louis, MO, USA), and the plates were imaged on a CYTATION3 imaging reader (BioTek Instruments) in 2 × 2 montage mode.
Screening data analysis and hit selection
For the quality control metric in siRNA screening, Z’ factor is used which is defined as follows16:
where SD is for standard deviation and AVG is for average.
The range of z’-factor is negative infinity to one, with > 0.5 indicating an excellent assay and > 0 a borderline assay.
Raw data were converted into log2 scale to calculate the inhibitory effect of each siRNA: cell count and cell viability compared to the negative controls per plate. After normalization, negative values indicate that treating the target gene has a lower number of cells than the negative controls. All data were on a log2 scale, unless stated otherwise. Statistical significance was calculated by the independent 2-sample Student’s t test. Inhibitory siRNA hits were selected by combination of cisplatin and siGPCR transfection. The cisplatin-sensitive hits were selected with a p-value < 0.01 in combined treatment with cisplatin. The common hits were selected by p-value < 0.01 in both single and combined treatment with cisplatin. We calculated the cell counting value as a percentage for siNC-DMF. All data analysis including t test is done using Excel.
Validation experiments of hit genes
Validation was performed to identify false positives. siRNAs from On-Target Plus siRNAs (siADRA2A, siEMR3, siF2RL3, siGPR108, siNPSR1, siNPY and siTACR3) were rescreened with A549 using the same transfection protocol of 10 nM by reverse transfection using Lipofectamine RNAi Max and Opti-MEM with DMF and a concentration of 1 µM cisplatin according to the manufacturer’s protocols. Repeated experiments were performed twice. Another validation experiment was conducted on five siRNAs (siEMR3, siF2RL3, siGPR108, siNPSR1 and siTACR3) from siTOOLs Biotech with A549, EKVX, H1437, H1793. siRNAs were transfected (final concentration of 4 nM) by reverse transfection using Lipofectamine RNAi Max and Opti-MEM and a concentration of 1 µM cisplatin with DMF. The experiment was repeated four times. For the anticancer effect, % Growth inhibition was defined as follows:
Self-renewal experiment
A self-renewal experiment was conducted to identify how the pretreated chemical can affect the regeneration and growth of cancer cell lines even though it was replaced with fresh medium. We seeded the A549 cell line in a 96-well plate (Thermo Fisher) (2500 cells per well) for pretreatment. Transfection of siNC and siGPCRs was performed. After 24 h, the cells were treated with 1 µM cisplatin and DMF to identify four conditions (siNC-DMF, siNC-cisplatin, siGPCR-DMF and siGPCR-cisplatin). After a total of 96 h, the cells were harvested and seeded in 384-well plates by seeding number (40, 80, 120, 200, 400, 800, 1200 and 2000 cells per well) in fresh medium. After 96 h, the cells were assayed using Hoechst 33,342 (Sigma–Aldrich) to count the cells.
siRNA transfection of cisplatin-pretreated A549 cells
For transfection of cisplatin-pretreated A549, we performed the same protocol with the A549 cell line prepared in advance. Cisplatin (1 µM) in the culture media was added to prepare cisplatin-pretreated A549 cells. We normalized the cell counting value as a percentage for siNC. The experiment was conducted with three repeats.
Survival analysis
RNA sequencing data, mutation data and clinical data were obtained from The Cancer Genome Atlas (TCGA) GDC data portal23. The RNA sequencing data in FPKM (Fragments Per Kilobase Million) were converted to log2 (TPM + 1) (Transcripts Per Kilobase Million). Both FPKM and TPM indicate expression levels of RNA transcript. TPM was introduced as a measurement to correct the inconsistences among independent samples24. For calculation of survival analysis, the following patient details were obtained: vital status, gender, days to death, days to last follow-up and days to new tumor event after initial treatment from clinical data.
The genes were analyzed using the Kaplan–Meier method25 and the log-rank test26 via R. Gender (female, male and both) and stage (I, II, III and IV) were divided by TCGA clinical data. The patients were divided into high expression and low expression groups based on the median and quartile (high group > top 25%, low group < bottom 25%) gene expression among all patients for each gene. Overall survival (OS) was defined as the length of time from either the date of diagnosis or the start of treatment for a disease, such as cancer, to the date of death, and patients diagnosed with the disease who were still alive were censored. Disease-free survival (DFS) was defined from randomization to the first evidence of recurrence, second primary malignancy or death, whichever occurred first. To obtain a p-value indicating the significance of this analysis, the log-rank test26 was performed.
Prognostic RNA expression
Survival analysis was calculated for 48 different measures using the TCGA dataset. The patients were divided according to the clinical data, including gender (female, male, and both) and stage (I, II, III, and IV). Gene expression groups were divided into medians or quartiles and calculated according to OS and DFS. Among the 48 survival p-values calculated in this way, the best p-value was used. We analyzed favorable and unfavorable prognostic gene expression27. Favorable prognostic gene expression was associated with a lower survival rate in the group with low gene expression, and unfavorable prognostic gene expression was associated with a lower survival rate in the group with high gene expression.
Gene ontology database
We used GO (Gene Ontology) term to find out with which function each group of genes is associated. Among them, we focused on the biological process term. GO database were obtained from msigdb (https://www.gsea-msigdb.org/gsea/msigdb/).
Oncoprint
Oncoprint was used to display mutation information for LUAD (Lung Adenocarcinoma) patients whose mutation information were obtained from TCGA. Data processing was done via R.
Data availability
All data generated or analyzed during this study are included in this published article and its supplementary information files.
References
Yang, D. et al. G protein-coupled receptors: structure- and function-based drug discovery. Signal Transduct. Target Ther. 6, 7. https://doi.org/10.1038/s41392-020-00435-w (2021).
Hauser, A. S., Attwood, M. M., Rask-Andersen, M., Schioth, H. B. & Gloriam, D. E. Trends in GPCR drug discovery: New agents, targets and indications. Nat. Rev. Drug Discov. 16, 829–842. https://doi.org/10.1038/nrd.2017.178 (2017).
Sriram, K. & Insel, P. A. G Protein-coupled receptors as targets for approved drugs: How many targets and how many drugs?. Mol. Pharmacol. 93, 251–258. https://doi.org/10.1124/mol.117.111062 (2018).
Saikia, S., Bordoloi, M. & Sarmah, R. Established and in-trial GPCR families in clinical trials: A review for target selection. Curr. Drug Targets 20, 522–539. https://doi.org/10.2174/1389450120666181105152439 (2019).
Yang, S. et al. Crystal structure of the Frizzled 4 receptor in a ligand-free state. Nature 560, 666–670. https://doi.org/10.1038/s41586-018-0447-x (2018).
Chaudhary, P. K. & Kim, S. An insight into GPCR and G-proteins as cancer drivers. Cells 10. https://doi.org/10.3390/cells10123288 (2021).
Fournel, L. et al. Cisplatin increases PD-L1 expression and optimizes immune check-point blockade in non-small cell lung cancer. Cancer Lett. 464, 5–14. https://doi.org/10.1016/j.canlet.2019.08.005 (2019).
Oliver, T. G. et al. Chronic cisplatin treatment promotes enhanced damage repair and tumor progression in a mouse model of lung cancer. Genes Dev. 24, 837–852. https://doi.org/10.1101/gad.1897010 (2010).
Jeong, E. et al. Dissecting phenotypic responses of the druggable targetome in cancers. Sci. Rep. 9, 12513. https://doi.org/10.1038/s41598-019-48989-2 (2019).
Meyers, R. M. et al. Computational correction of copy number effect improves specificity of CRISPR-Cas9 essentiality screens in cancer cells. Nat Genet. 49, 1779–1784. https://doi.org/10.1038/ng.3984 (2017).
McDonald, E. R., 3rd et al. Project DRIVE: A compendium of cancer dependencies and synthetic lethal relationships uncovered by large-scale, deep RNAi screening. Cell 170, 577–592 e510. https://doi.org/10.1016/j.cell.2017.07.005 (2017).
Song, M. et al. Analysis of image-based phenotypic parameters for high throughput gene perturbation assays. Comput. Biol. Chem. 58, 192–198. https://doi.org/10.1016/j.compbiolchem.2015.07.005 (2015).
Song, M. et al. Loss-of-function screens of druggable targetome against cancer stem-like cells. FASEB J. 31, 625–635. https://doi.org/10.1096/fj.201600953 (2017).
Teng, J. P. et al. Gemcitabine and cisplatin for treatment of lung cancer in vitro and vivo. Eur. Rev. Med. Pharmacol. Sci. 22, 3819–3825. https://doi.org/10.26355/eurrev_201806_15266 (2018).
Shi, S. et al. ER stress and autophagy are involved in the apoptosis induced by cisplatin in human lung cancer cells. Oncol. Rep. 35, 2606–2614. https://doi.org/10.3892/or.2016.4680 (2016).
Mazur, S. & Kozak, K. Z’ factor including siRNA design quality parameter in RNAi screening experiments. RNA Biol. 9, 624–632. https://doi.org/10.4161/rna.19759 (2012).
Lee, J. et al. Q-omics: smart software for assisting oncology and cancer research. Mol. Cells 44, 843–850. https://doi.org/10.14348/molcells.2021.0169 (2021).
Jeong, E., Lee, Y., Kim, Y., Lee, J. & Yoon, S. Analysis of cross-association between mRNA expression and RNAi efficacy for predictive target discovery in colon cancers. Cancers (Basel) 12. https://doi.org/10.3390/cancers12113091 (2020).
Brown, S. V., Hosking, P., Li, J. & Williams, N. ATP synthase is responsible for maintaining mitochondrial membrane potential in bloodstream form Trypanosoma brucei. Eukaryot Cell 5, 45–53. https://doi.org/10.1128/EC.5.1.45-53.2006 (2006).
Ni, Z. et al. CLPTM1L is overexpressed in lung cancer and associated with apoptosis. PLoS ONE 7, e52598. https://doi.org/10.1371/journal.pone.0052598 (2012).
Nowak, G. & Bakajsova, D. Assessment of mitochondrial functions and cell viability in renal cells overexpressing protein kinase C isozymes. J. Vis. Exp. https://doi.org/10.3791/4301 (2013).
Obata, K. et al. Tachykinin receptor 3 distribution in human oral squamous cell carcinoma. Anticancer Res. 36, 6335–6341. https://doi.org/10.21873/anticanres.11230 (2016).
Cancer Genome Atlas Research, N. et al. The Cancer Genome Atlas Pan-Cancer analysis project. Nat Genet 45, 1113–1120. https://doi.org/10.1038/ng.2764 (2013).
Wagner, G. P., Kin, K. & Lynch, V. J. Measurement of mRNA abundance using RNA-seq data: RPKM measure is inconsistent among samples. Theory Biosci. 131, 281–285. https://doi.org/10.1007/s12064-012-0162-3 (2012).
Bland, J. M. & Altman, D. G. Survival probabilities (the Kaplan-Meier method). BMJ 317, 1572. https://doi.org/10.1136/bmj.317.7172.1572 (1998).
Bland, J. M. & Altman, D. G. The logrank test. BMJ 328, 1073. https://doi.org/10.1136/bmj.328.7447.1073 (2004).
Uhlen, M. et al. A pathology atlas of the human cancer transcriptome. Science 357. https://doi.org/10.1126/science.aan2507 (2017).
Acknowledgements
This research was funded by the National Research Foundation of Korea (KRF) [NRF-2018R1A2B6009313 to EJ and NRF-2022R1F1A1062559 and NRF-2016R1A5A1011974 to SY].
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Y.K. and J.L.—performed screening and validation experiment, prepared the manuscript. S.J.—contributed data and analysis tools. W.K.—analyzed data and interpreted results. E.J.—conceived and designed the analysis, contributed data and analysis tools, and participated in writing of the paper. S.Y.—conceived and designed the whole study, interpreted results, and wrote the paper. All authors read and approved the final version.
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Kim, Y., Lee, J., Jeong, S. et al. Screening of the siGPCR library in combination with cisplatin against lung cancers. Sci Rep 12, 17358 (2022). https://doi.org/10.1038/s41598-022-21063-0
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DOI: https://doi.org/10.1038/s41598-022-21063-0
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