Abstract
Understanding the Earth’s climate system during past periods of high atmospheric CO2 is crucial for forecasting climate change under anthropogenically-elevated CO2. The Mesozoic Era is believed to have coincided with a long-term Greenhouse climate, and many of our temperature reconstructions come from stable isotopes of marine biotic calcite, in particular from belemnites, an extinct group of molluscs with carbonate hard-parts. Yet, temperatures reconstructed from the oxygen isotope composition of belemnites are consistently colder than those derived from other temperature proxies, leading to large uncertainties around Mesozoic sea temperatures. Here we apply clumped isotope palaeothermometry to two distinct carbonate phases from exceptionally well-preserved belemnites in order to constrain their living habitat, and improve temperature reconstructions based on stable oxygen isotopes. We show that belemnites precipitated both aragonite and calcite in warm, open ocean surface waters, and demonstrate how previous low estimates of belemnite calcification temperatures has led to widespread underestimation of Mesozoic sea temperatures by ca. 12 °C, raising estimates of some of the lowest temperature estimates for the Jurassic period to values which approach modern mid-latitude sea surface temperatures. Our findings enable accurate recalculation of global Mesozoic belemnite temperatures, and will thus improve our understanding of Greenhouse climate dynamics.
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Introduction
Accurately reconstructing the Earth’s climate through geological time is important for understanding Earth system feedbacks and for forecasting future climate change1. In particular, past periods of highly elevated atmospheric CO2, where Greenhouse conditions prevailed, may provide important insights into climate processes operating under anthropogenically elevated CO2. The middle and late Mesozoic (Jurassic and Cretaceous; 201–66 Ma) saw such warm-climate processes, wherein polar temperatures were so high that polar ice-caps were absent most of the time2. Reconstructing sea and land temperatures during this interval remains challenging. Climate proxies applied to younger sediments (e.g. ice-core and tree-ring records, alkenone biomarkers) are unavailable this far back in time, and many Mesozoic sediments have undergone significant post-depositional thermal and diagenetic alteration, modifying the original composition of fossils. Nonetheless, biogenic calcite from organisms such as brachiopods, bivalves, and particularly belemnites is frequently preserved in such sediments, enabling the application of oxygen isotope thermometry for sea-water temperature reconstructions. Belemnites, emerging in the earliest Jurassic, were squid-like cephalopods (Mollusca) that built their internal skeleton from calcite and aragonite, and went extinct at the end of the Cretaceous. Their ubiquity within Jurassic and Cretaceous seas, and the high preservation potential of their low-Mg calcite skeleton (rostra), make them a favoured target for temperature reconstructions via oxygen isotope thermometry3,4,5,6,7,8,9,10,11,12. However, two major limitations to this method have led to uncertainties in Mesozoic temperature reconstructions. The first is that the oxygen isotope composition of skeletal carbonates in marine systems vary as a function of both the ambient temperature and oxygen isotope composition of the seawater (δ18Osw). Mesozoic δ18Osw cannot be measured directly, and is usually assumed to be the average value for seawater in an ice-free world (-1‰ SMOW13) ; yet the δ18Osw value at a given locality and depth in the ocean may deviate from this average by as much as 4‰14,15. The second limitation is that numerous equations have been determined for the relationship between temperature and δ18O in different calcite types, e.g. molluscan calcite16,17,18, brachiopod calcite19,20, foraminifera21,22, barnacle calcite23, meteoric speleothems and cements24,25,26,27,28, and synthetic calcite29,30. It is not known if belemnites fractionated 18O to the same extent as modern biotic carbonates, and therefore which equation, if any, is appropriate for belemnite calcite. The molluscan equation of Anderson and Arthur18 or the general synthetic calcite equation of Kim and O'Neil30 are most commonly used for belemnite calcite temperature reconstructions, yet it is observed that belemnites often give similar, or, in many cases, lower temperatures (i.e. higher δ18Ocalcite) than co-occurring benthic biotic calcites3,4,5,6,8,11,31. It has been hypothesised that belemnites may have been migratory to areas of colder and/or isotopically distinct waters31,32, or that early diagenetic infill (i.e. as the belemnite skeleton lay on the seafloor) biases belemnite calcite to colder temperatures33, although this is disputed by other geochemical studies34,35. Furthermore, clumped isotope studies comparing the (visibly) porous apical area to the (less visibly porous) rest of the belemnite rostrum return colder temperatures for the apical area, contradicting a diagenetic cause35. Biomarker-based (TEX86) temperature reconstructions for shallow-buried (i.e. especially immature) Mesozoic sections similarly give much warmer temperatures than belemnite rostra7,36. As the TEX86 method is thought to record sea surface temperatures only, it was argued that the belemnites may have been nektonic or nektobenthic, living mostly below the thermocline in colder waters than the surface7,31. Yet this does not explain how belemnites may give colder temperatures than co-occurring benthic biotic calcites3,6,8,11,31. Clumped isotope thermometry reconstructs belemnite calcite temperatures closer to sea surface temperatures than bottom water temperatures, considerably warmer than oxygen isotope thermometry35,37,38,39,40,41. This suggests that either belemnites inhabited highly saline waters (with δ18Osw elevated by evaporation), or that the standard calcite thermometry equations are inappropriate for belemnite calcite35,37,38,39,40,41.
Here we present new clumped isotope data derived from co-occurring calcite and aragonite in individual exceptionally well preserved belemnites (Cylindroteuthis)42 from the Callovian-aged Christian Malford Lagerstätte (Fig. 1). These samples are compared to other (non-belemnite) aragonites from the same horizon in order to evaluate the putative temperature record based upon the oxygen isotopic composition of well-preserved marine fossils, and thereby to resolve which oxygen thermometry equation is most appropriate for belemnite rostra in Mesozoic paleoclimate studies. In doing so, we highlight significant systematic underestimations of global sea temperature in Mesozoic reconstructions. Our warm temperatures are more in line with the geographical distributions of temperature-sensitive fossil flora and fauna, as well as the results of climate models with increased CO2 levels, and demonstrate that at least several common belemnite genera lived neither deep in the water column nor in hypersaline conditions.
Results
Preservation
The Christian Malford Lagerstätte is known for its exceptional preservation of biomaterials in fossil marine organisms42,43,44. The host sediments have experienced minimal burial and thermal maturation as indicated by the immaturity of their organic matter43,45,46. Powder X-Ray diffraction (PXRD) confirms the preservation of original aragonite (Supplementary Fig. S3), which is uncommon in sediments this old due to the metastability of aragonite at Earth surface temperatures and pressures47, and further confirms the extremely low thermal maturity. Belemnite rostral calcite shows extremely low Mn/Ca and Fe/Ca values (Supplementary Fig. S12), in agreement with Price et al.42, and electron backscatter diffraction (EBSD) and scanning electron microscope (SEM) electron dispersive spectra (EDS) element maps show that no perceptible diagenetic alteration occurred in the non-apical areas of the belemnite rostra (Supplementary Figs. S7, S8, S9, S10, S11).
Whilst the aragonite and calcite appear texturally and geochemically pristine, studies have shown that clumped isotope (Δ47) temperatures may be increased by re-ordering of the 13C-18O bonds (‘solid state reordering’), a process by which no minor element or visible change, even at the microscopic level, occurs in the carbonate48,49,50. For calcite formed at ambient temperatures, this re-ordering may occur where the samples experience temperatures above 80–100 °C over geological timescales (millions of years)49,50,51. Aragonite is much more prone to alteration and its reordering kinetics are much faster than those of calcite48; e.g. Ritter et al.52 showed that such reordering may occur after 20 weeks of the aragonite being held at 100 °C. However, for the Callovian sediments at Christian Malford, burial estimates, maturity indices and diagenetic carbonate clumped isotope data suggest that such temperatures were not reached43,45,46, negating this effect. In combination, these conditions provide a unique opportunity to determine the accuracy with which belemnite rostra record original calcification temperatures and can be relied upon to faithfully record ambient marine conditions in the Mesozoic. If aragonite and calcite from the same organism yield the same temperature, we can be very confident that they represent true original temperatures, as any alteration would preferentially disrupt the values for the aragonite over the calcite.
Clumped isotope palaeothermometry
Clumped isotope compositions for the analysed calcites and aragonites range from 0.580 to 0.609 (I-CDES53; excluding separated diagenetic calcites from the apical area and spar cements), which correspond to temperatures ranging from 20.0 to 29.5 °C based on the Anderson et al.54 equation. Anderson et al.54 found that inorganic, and most biogenic, carbonates (including aragonite) show the same temperature dependence; thus, their equation can confidently be applied to reconstruct palaeotemperatures for aragonites and calcites. Reconstructed aragonite and calcite Δ47 palaeotemperatures from the same belemnite are consistently within error of each other, and are in agreement with other biotic aragonites from the sample (Fig. 2A). This supports that the observed Δ47 temperatures are pristine. This is therefore the first study that provides seawater temperatures and oxygen isotope compositions which are proven to be unaffected by reordering.
Discussion
This study, along with other clumped isotope studies of belemnites35,37,38,39,40,41 finds that belemnites grew in much warmer (i.e. near-surface) waters than previous stable isotope studies have suggested3,4,5,6,7,8,9,10,11,12. Clumped isotope records show that belemnites record warmer temperatures than early (seafloor) diagenetic cements35 (Fig. 2A), consistent with recent palaeontological work that shows that some belemnites lived in the top 200 m of the water column55.
Traditional stable oxygen isotope thermometry (e.g. Anderson and Arthur18 equation, assumed δ18Osw = − 1 ‰ for an ice-free world13) consistently returns temperatures for rostral calcite that are, on average, 9 °C colder than for phragmocone aragonite (using the Grossman and Ku56 aragonite equation) from the same individual belemnite (Fig. 2). Whilst this phenomenon has previously been observed42, it was not possible to determine if the aragonite temperatures were too warm or if the Anderson and Arthur18 equation gives temperatures that are too cold. It is believed that belemnite calcite precipitated near Δ47 isotopic equilibrium41,57, and the consistency between belemnite calcite and aragonite clumped isotope temperatures (Fig. 2) suggests that belemnite aragonite also does not exhibit strong kinetic disequilibrium effects with respect to Δ47, despite this phenomenon having been observed in modern cephalopod aragonite58,59. In modern cephalopods, disequilibrium effects lower Δ47 values, i.e. yielding temperatures that are warmer than the true growth temperature, by as much as 8 °C58,59, yet belemnite aragonite in this study does not yield warmer temperatures than the rostral calcite (Fig. 2A). Thus we proceed under the assumption that both calcite and aragonite from belemnites precipitated in Δ47 equilibrium.
Several more recent studies present equations determined for natural calcite δ18O grown in equilibrium. Two studies present equations derived from very slow-growing subaqueous calcites26,28. Daëron et al.28, being the most recent, is given below (Eq. 1). Another stable isotope equation derived for fast-growing travertines, is believed to reflect (near) equilibrium conditions27, yet differs from Daeron et al.28 in having a slightly steeper slope (Eq. 2):
where α is the calcite/water oxygen-18 fractionation factor and T is the absolute temperature in Kelvin.
We do not know the δ18O aragonite-water fractionation for belemnite phragmocone, yet for modern aragonitic cephalopods this effect is negligible, and closely approximates both the equilibrium calibration of Daëron et al.28, and the biogenic calibrations of White et al.60 and Grossman and Ku56,58,59. However, calcites may show near-equilibrium Δ47 yet far-from-equilibrium δ18O61, and as there are no studies on calcifying cephalopods, we do not know if the biotically-mediated precipitation of rostral calcite may have been fractionated with respect to seawater. As clumped isotope thermometry gives both the temperature and δ18Ocarbonate, these can be used together to back-calculate the δ18Osw, using one of the stable isotope equations. It is hard to reconcile two carbonate phases from the same organism growing in different δ18Osw; thus, we can select the equations that yield the most similar δ18Osw for belemnite calcite and aragonite pairs (Fig. 2D). The closest matches are between the Kele et al.27 equation for the rostra and any of the aragonite equations28,56,60 (Fig. 2D) for the phragmocone; closely followed by Daëron et al.28 for the rostral calcite with Grossman and Ku56 for aragonite (Fig. 2D and Supplementary data). Interestingly, applying Daëron et al.28 to both calcite and aragonite gives a significant offset between reconstructed δ18Osw—the aragonite is c. 1.5 ‰ lighter than the calculated calcite average δ18Osw (Fig. 2D and Supplementary data). The Kele et al.27 equation, derived for fast-growing travertines, is believed to reflect (near) equilibrium conditions, yet differs from Daëron et al.28 in having a slightly steeper slope (Eq. 2). Kele et al.27 could not explain the different slope of the travertine curve by any physical or chemical parameter (including growth kinetics). For belemnite calcite, the close fit to the Kele et al.27 equation may imply that some biotically-driven fractionation of 18O occurred during precipitation of the calcite from the belemnite body fluid (since the aragonite was precipitated from body fluid in equilibrium with seawater28).
At Christian Malford (Phaeinum subzone), it happens that the δ18Osw average that is calculated for both aragonite and calcite is approximately − 1‰, the average expected ocean value for an ice-free world13. When using clumped temperatures and measured δ18O carbonate at other sites (Fig. 3 and references therein), we see greater deviations from this global average value, indicating that using a global average value is not representative of all localities, particularly at high and low latitudes and in semi-enclosed basins. Indeed, if another stable isotope equation is used for the belemnite calcites, we still see the large spread in reconstructed δ18Osw (as great as 7‰35,38,40, Fig. 3). It is clear from the present variation in oxygen isotope composition of surface waters, and from GCM models of δ18Osw, that applying a global average is unlikely to be representative of a particular location or water depth14,15. Alberti et al.62 proposed using an empirically derived equation for modern oceans, − 1‰ to account for the absence of Polar ice, to estimate latitudinal δ18Osw trends. However, as highlighted by Alberti et al.62, this does not take into account paleogeography, therefore it is unlikely to capture local δ18Osw variations, particularly for the semi-enclosed basins that characterised the Mesozoic of Europe during the Jurassic (Fig. 1B). Using clumped isotopes temperatures to back-calculate δ18Osw reveals that there are large variations in local δ18Osw that are not captured using either a global average or empirical gradients based on modern geography (Fig. 3). The studies of Wierzbowski et al.38 (Russian Platform) and Vickers et al.35 (Hebrides Basin) span broad age ranges and encompass changes in circulation patterns, as reflected in their very large ranges in δ18Osw values (Fig. 3). The study of Price et al.40 demonstrates that for a single time-slice, latitudinal δ18Osw as reconstructed by clumped isotope thermometry does show higher values at the equator than the poles (i.e. more similar to that proposed by Alberti et al.62), yet there is a broad and varied spread in the mid-latitudes. In the study of Price et al.40 some of the spread may arise from analysing different belemnite genera and species, which may have inhabited different depth habitats. Yet, since large ranges are observed in studies that use only one or two belemnite genera or families35,38,39, we believe that a significant portion of the variation arises from local δ18Osw fluctuations. Thus, we anticipate that the majority of published values are still likely to under- or over-estimate local δ18Osw, leading to an over- or under-estimation in seawater temperatures, even if using the Kele et al.27 equation to calculate sea temperatures.
To conclude, we provide the first seawater Δ47 temperatures for the Jurassic based on samples that most likely have not been modified by diagenetic processes nor by 13C-18O bond reordering. We show that the palaeothermometry equations that have traditionally been applied to belemnite calcite are inappropriate, and, therefore, that reconstructions based on them grossly underestimate palaeotemperatures for the Mesozoic. The present study implies that ca. 11–12 °C needs to be added to the temperature estimates for the Jurassic and Cretaceous that were based on belemnites4,5,6,7,8,9,10,11,12 taking the average δ18Ocalcite of combined belemnite datasets and the 2 standard deviation range12. This has substantial implications for our understanding of the Mesozoic world and Greenhouse Earth-system states. It raises some of the lowest temperature estimates for the Jurassic period (such as the Bajocian “cold mode”; ~ 7 °C) to values which approach modern mid-latitude sea surface temperatures, thereby undermining previously speculated “icehouse” phases9. The warm belemnite temperatures do not contradict cooler temperature estimates from co-occurring benthic organisms; rather, they may be used to understand vertical temperature profiles in the oceans.
Methods
The carbonate material used in this study all comes from a 2 m thick interval in the Callovian-aged Peterborough Member of the Oxford Clay Formation (Athleta Zone, Phaeinum subzone). The Peterborough Member consists of alternating organic-poor, shell-rich massive clay and organic-rich, variably shelly, fissile clay42,66 All material was collected from an excavation site at Christian Malford, Wiltshire, U.K. (Fig. 1). Published ICP-MS, SEM and CL work on selected Cylindroteuthis belemnite aragonite and calcite material indicates the exceptional quality of preservation of these carbonates42, as does the PXRD, ICP-OES, SEM, EBSD and EDS element maps presented in this study (Supplementary material Figs. S2, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12). Estimates suggest a maximum burial depth of only c. 500 metres45, indicating that significant post-depositional heating did not occur. Due to the limited amount of some of the aragonitic material (particularly phragmocones) it was not possible to analyse all samples for ICP-OES, SEM and PXRD, yet it is assumed that the analysed samples are representative for all samples used in this study because minimal variability was observed.
PXRD
Powder X-ray diffraction (PXRD) was carried out using a Stoe StadiP transmission (capillary) diffractometer with a copper anode at 30 mA, 40 kV and a germanium 111 monochromator to produce Kα1 X-rays. The diffracted beam was collected by an 18° 2θ Dectris Mythen1K silicon strip detector. Samples were loaded in 0.3 mm borosilicate glass capillaries, mounted and aligned on the goniometer head and set to spin continuously during data collection. Both data sets were scanned from 10 to 55° 2θ stepping at 0.5° and 5 s/step. The resultant raw data has a step of 0.015° 2θ. Machine alignment was monitored using an NBS silicon standard. Phase analysis was done using Bruker’s “Eva” program67 interfaced with the Powder Diffraction File provided by the International Centre for Diffraction Data.
Microscopy
Scanning electron microscopy was undertaken on selected samples, in order to assess whether the original biomineral crystal habits are preserved, and to identify the best-preserved regions within the belemnite rostra. Analyses were performed using secondary electrons on an FEI Quanta Inspect 250 Scanning Electron Microscope under a high vacuum of 2.40 to 2.93·10–4 Pa and an electron beam of 95–97 μA at the Geological Museum in Copenhagen, out on selected aragonites from phragmocones, ammonites and bivalves analysed in this study.
For SEM–EDS and EBSD analysis, cross- and longitudinal- sections of a selected rostra were mounted in epoxy and mechanically polished down to a 0.25 µm diamond solution grain size, followed by chemical–mechanical polishing using an alkaline solution of colloidal silica in a neoprene substrate. EBSD orientation mapping was performed on the coated sample (~ 2.5 nm carbon) in a Thermo Fischer—FEI Quanta 200F equipped with an EDAX Hikari EBSD camera and TEAM software for data acquisition at the Scientific Center for Optical and Electron Microscopy (ScopeM) at ETH Zurich. Acquisition was performed with an accelerating voltage of 20 kV, beam current of 8 nA, working distance of 17 mm, and mapping step size of 1 µm. Post-acquisition cleaning included grain confidence index (CI) standardization followed by one step of grain CI correlation. All points with CI < 0.1 and grains with less than 10 pixels were removed to prevent artifacts in the calculations.
The SEM–EDS element maps were undertaken at the SEM laboratory at the Geological Survey of Denmark and Greenland (GEUS), which hosts a ZEISS sigma 300VP field emission scanning electron microscope that is equipped with 2 Bruker Xflash 6|30 129 eV EDS detectors and a Bruker e-Flash FS EBSD detector. Element maps were acquired from infilled apical area to the outermost pyritised rim of the rostrum, covering the changes of growth ring density and mineralogical changes. The sets of transect section were obtained for the cross- and the longitudinal-section. Elements mapped (Ca, Mg, Fe, Mn, O) cover the range of possible carbonates, with aragonite distinguished by trace Sr; the potential occurrence of apatite (P), pyrite (Fe, S), clays (Al, Si, Ba, K) and quartz (Si) was also tested.
ICP-OES
Minor element analyses were performed using an Agilent 5110 VDV ICP-OES at the Camborne School of Mines, University of Exeter, following methods laid out in detail in Ullmann et al.68. The minor element data are expressed as ratios to Ca. Fossil samples were dissolved in 2% v/v HNO3 with a dilution factor of ~ 16,000, yielding a nominal Ca concentration of 25 µg/g in solution. Signal quantification was carried out using a four point calibration using a blank solution and three matrix matched calibration solutions made up from certified single element standards mixed to match the chemical composition of the analysed samples. Accuracy and precision of the analyses was controlled by multiple measurements of interspersed international reference materials (JLs-1 and AK) and a quality control solution (BCQ2). Repeatability of the measurements is generally better than 1% (2 relative standard deviations) unless affected by limited count rates (el/Ca < c. 100 times the quantification limit). Repeatability of element/Ca ratios in the latter case are 1 µmol/mol for Mn/Ca, 3 µmol/mol for Fe/Ca, 0.3 mmol/mol for S/Ca and 0.2 mmol/mol for P/Ca. Quantification limits for the measurements computed as six times the standard deviation of the measurement blank are 9 µmol/mol for Mg/Ca, 0.2 µmol/mol for Sr/Ca, 1 µmol/mol for Mn/Ca, 4 µmol/mol for Fe/Ca, 0.4 mmol/mol for S/Ca and 0.2 mmol/mol for P/Ca. Due to the generally limited quantity of phragmocone material available, it was not possible to analyse all phragmocone samples for ICP-OES. ICP-OES data are reported and plotted in the Supplementary Material.
Clumped isotope thermometry
Powdered homogenized rostrum samples were collected from across the middle chambers (apex and outer edge avoided), away from the tip of the rostrum, using a Dremel drill. For the aragonitic material (phragmocones and ammonites), small pieces were picked off using tweezers, and powdered using an agate mortar and pestle. With the phragmocones, it was not possible to select specific chambers or homogenize across many chambers, due to limited aragonitic belemnite material remaining.
Clumped isotope measurements were carried out at the ETH Zurich using a ThermoFisher Scientific MAT253 mass spectrometer coupled to a Kiel IV carbonate preparation device, following the methods described in Müller et al.69. The Kiel IV device included a PoraPakQ trap kept at -40 °C to eliminate potential organic contaminants. Samples were measured between May 2019 and December 2020 by measuring maximum 3 replicates of each sample per run which consists generally of 24 samples of 130–150 µg interspersed with 20 replicates of each of the three carbonate standards ETH-1, ETH-2 and ETH-370. The samples were analysed in LIDI mode with 400 s of integration of sample and reference gas. The calculations and corrections were done with the software Easotope71 using the revised “Brand parameters” for 17O correction as suggested by Daëron et al.64. The data are reported with respect to the Intercarb carbon dioxide equilibration scale (I-CDES)53. Temperatures were calculated using the Anderson et al.54 calibration which is based on the re-measurement of a series of samples that were used for previous calibrations. Their consistency with calibrations based on biogenic carbonates72,73,74 suggest that it is appropriate for marine biogenic carbonates. Corrections for aragonite were the same as for calcite except that we used the phosphoric acid oxygen isotope correction for aragonite of 1.0090975.
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Acknowledgements
Funding for this study was gratefully received from the Danish Council for Independent Research -Natural Sciences grant DFF-7014-00142 to C. Korte. We extend thanks to Martin Vickers, Department of Chemistry, UCL, London, for overseeing the Powder X-Ray Diffraction analysis on the carbonates. We would like to thank Madalina Jaggi at ETH Zurich for running the clumped isotope analyses on the carbonate samples. We thank our reviewers, Matthias Alberti and anonymous, and editorial board member Slah Boulila, for their constructive feedback reviewing the manuscript.
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M.L.V. contributed the ideas, the main writing of the manuscript, preparation of all figures, and took part in sample preparation, analysis and interpretation for all methods. S.M.B. ran and interpreted the clumped isotope analysis at ETH Zurich, and wrote the methods and parts of the interpretation of the clumped isotope data in the manuscript. C.V.U. ran ICP-OES analysis at CSM, Penryn, and assisted in the interpretation of the data, and writing of the methods. S.L. undertook the preparation of the belemnite thick sections, and SE and EDS SEM mapping, with interpretation thereof. N.L. and L.G.M. undertook the EBSD analysis and interpretation of the EBSD data, and writing of the methods. G.D.P. and P.R.W. collected the samples and ran preliminary preservational quality checks on the material, and wrote the geological setting. I.W.H. undertook back-scattered electron SEM analysis of selected samples and interpretation thereof. S.P.H. provided interpretation of the data and contributed to the writing of the manuscript. C.K. provided the funding for the research, and contributed to the interpretation of the data. All authors reviewed the manuscript.
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Vickers, M.L., Bernasconi, S.M., Ullmann, C.V. et al. Marine temperatures underestimated for past greenhouse climate. Sci Rep 11, 19109 (2021). https://doi.org/10.1038/s41598-021-98528-1
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DOI: https://doi.org/10.1038/s41598-021-98528-1
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