Cardiac automaticity is set by pacemaker activity of the sinus node (SAN). In addition to the ubiquitously expressed cardiac voltage-gated L-type Cav1.2 Ca2+ channel isoform, pacemaker cells within the SAN and the atrioventricular node co-express voltage-gated L-type Cav1.3 and T-type Cav3.1 Ca2+ channels (SAN-VGCCs). The role of SAN-VGCCs in automaticity is incompletely understood. We used knockout mice carrying individual genetic ablation of Cav1.3 (Cav1.3−/−) or Cav3.1 (Cav3.1−/−) channels and double mutant Cav1.3−/−/Cav3.1−/− mice expressing only Cav1.2 channels. We show that concomitant loss of SAN-VGCCs prevents physiological SAN automaticity, blocks impulse conduction and compromises ventricular rhythmicity. Coexpression of SAN-VGCCs is necessary for impulse formation in the central SAN. In mice lacking SAN-VGCCs, residual pacemaker activity is predominantly generated in peripheral nodal and extranodal sites by f-channels and TTX-sensitive Na+ channels. In beating SAN cells, ablation of SAN-VGCCs disrupted late diastolic local intracellular Ca2+ release, which demonstrates an important role for these channels in supporting the sarcoplasmic reticulum based “Ca2+ clock” mechanism during normal pacemaking. These data implicate an underappreciated role for co-expression of SAN-VGCCs in heart automaticity and define an integral role for these channels in mechanisms that control the heartbeat.
Heart automaticity is reliant on the primary pacemaker activity of the sino-atrial node (SAN) and impulse conduction through the principal components of the conduction system: the atrioventricular node (AVN), the His bundle and the Purkinje fibres network1. Under physiological conditions, the SAN generates cardiac automaticity whereas the AVN and the Purkinje fibres network initiate automaticity in case of SAN failure. Automaticity in cardiac cells is due to diastolic depolarization, a slow phase of the action potential cycle driving the membrane voltage from the end of the repolarization to the threshold of the following action potential2. While several aspects of SAN pacemaker mechanisms are still incompletely understood, there is general agreement that diastolic depolarization is generated by an interplay between the activity of ion channels of the plasma membrane and local diastolic intracellular Ca2+ release (LCR) from ryanodine receptors (RyRs) of the sarcoplasmic reticulum (SR)3,4,5.
Among plasmalemmal ion channels, hyperpolarization-activated “funny” f-channels of the HCN family6, voltage-gated Ca2+ channels (VGCCs)7, voltage-dependent tetrodotoxin (TTX)-sensitive Na+ channels mediating the neuronal Na+ current (INa(TTX))8,9,10,11 and transient receptor potential (TRPM and TRPC)12,13,14 channels have been shown to contribute to automaticity in the SAN or in the AVN. The contribution of RyR-dependent Ca2+ release to automaticity is currently interpreted in the framework of the “coupled clock” model of pacemaking3,15,16. Two functional components form the coupled clock: the spontaneous SR-based “Ca2+ clock” mechanism and the “membrane clock” (or voltage clock)15. A cyclical phenomenon of spontaneous voltage-independent LCR events generates the Ca2+ clock. Indeed, spontaneous LCRs can be observed in the absence of changes in membrane voltage and constitute the hallmark of the Ca2+ clock17. During pacemaking, spontaneous LCRs are generated by early diastolic openings of RyRs that activate the cardiac Na+–Ca2+ exchanger (NCX1)17,18. This electrogenic transport generates an inward current that contributes to diastolic depolarization19. Sequential and cyclical activation of plasmalemmal ion channels during pacemaking forms the membrane clock15,16. In the coupled-clock model of pacemaking, f-channels are important activators of the membrane clock at the beginning of diastolic depolarization15. In the last fraction of diastolic depolarization, T- and L-type Ca2+ channels activate in cooperation with NCX to drive the membrane voltage to the action potential threshold16. During the action potential phase, Ca2+ entry via L-type VGCCs refills the SR Ca2+ load, allowing new diastolic LCRs to occur in the following cycle. Therefore, under normal conditions, the Ca2+ clock and the membrane clock mutually entrain and synchronize to generate robust physiological pacemaking15,16 (see Refs.3,4,5 for review).
The adult mammalian heart expresses three VGCCs isoforms7: L-type Cav1.2 and Cav1.3 channels, as well as T-type Cav3.1 channels. These isoforms show differential expression in the working myocardium and in automatic tissue. In particular, adult ventricular myocytes express L-type Cav1.2 channels20, which couple excitation to contraction in the myocardium21. Cav1.2 channels are also ubiquitously expressed in the SAN region and in the conduction system20. In contrast, L-type Cav1.3 channels are highly expressed in automatic tissue20 and, in lower densities, in atria20,22. Finally, T-type Cav3.1 channels are not functionally expressed in the adult working myocardium but show strong expression in the SAN and in the AVN23. Cav1.3 functionally differs from the other cardiac L-type isoform. Under basal conditions, the threshold for activation of Cav1.3-mediated L-type Ca2+ current (ICaL) in SAN cells is significantly more negative (− 50 mV) than that of Cav1.2-mediated ICaL (− 30 mV)24, which controls the excitation–contraction coupling mechanism in the working myocardium. β-adrenergic activation shifts the threshold of both Cav1.3- and Cav1.2-mediated ICaL by ~ 5 mV to more negative voltages24. T-type Cav3.1 channels activate at more negative voltages than Cav1.3. In SAN cells, the threshold for activation of Cav3.1-mediated T-type Ca2+ current (ICaT) is more negative than that of Cav1.3-mediated ICaL (− 55 mV) and its half-inactivation voltage is more negative (− 70 mV)23,24. Previous studies showed that L-type Cav1.3 channels contribute to the generation of diastolic depolarization in the SAN24 and AVN25,26. In addition, we have showed that Cav1.3 channels contribute to the generation of diastolic LCRs and are necessary to synchronize LCRs events under β-adrenergic activation of pacemaking27. T-type Cav3.1 channels have also been shown to contribute to automaticity in the SAN24,28 and in the AVN25. Importantly, loss-of-function of Cav1.3 and Cav3.1 channels underlies congenital29 and autoimmune30 forms of SAN dysfunction associated with disturbances of atrioventricular conduction.
Despite this physiological relevance, it is currently unknown whether normal automaticity, heart rate and rhythm are contingent on the co-expression of L-type Cav1.3 and T-type Cav3.1 channels (hereby named SAN-VGCCs). In addition, genetic and functional evidence of the impact of co-expression of SAN-VGCCs within the coupled clock model are currently lacking. Here we hypothesised that co-expression of SAN-VGCCs is necessary to generate normal cardiac automaticity and to ensure synchrony between atrial and ventricular rhythmicity. We also hypothesised that, because of their negative voltage threshold for activation, SAN-VGCCs may constitute an important factor for generating the coupled clock pacemaker mechanism. To address this, we created a mouse model in which the two SAN-VGCCs expressed in the SAN and the AVN but not in the ventricle have been ablated (Cav1.3−/−/Cav3.1−/−). We demonstrate that co-expression of SAN-VGCCs is necessary to maintain pacemaking in the SAN and for proper impulse propagation, as well as to maintain normal impulse conduction and ventricular rhythmicity. We also show that SAN-VGCCs are necessary to generate late diastolic LCRs involved in the coupled clock pacemaker mechanism, a function that Cav1.2 channels cannot maintain in the absence of SAN-VGCCs.
Concomitant ablation of SAN-VGCCs abolishes VGCCs-mediated Ca2+ current in the voltage range of diastolic depolarization
We studied the consequences of concomitant ablation of SAN-VGCCs on total VGCCs-mediated current (ICa) in isolated SAN pacemaker cells from control and mutant (Cav3.1−/−, Cav1.3−/− and Cav1.3−/−/Cav3.1−/−) mice. ICa was first recorded from a holding potential (HP) of − 80 mV to record ICaL and ICaT23,24. Samples traces of total Ca2+ current (ICa) are reported in Fig. 1A for wild-type, Cav3.1−/−, Cav1.3−/− and Cav1.3−/−/Cav3.1−/− SAN cells. To separate ICaL from ICaT, we switched the HP to − 55 mV to inactivate ICaT (Fig. 1A). This HP inactivates about 15–20% of Cav1.3-mediated ICaL, but completely inactivates Cav3.1-mediated ICaT7. In wild-type SAN cells, net ICaT was calculated as the difference between traces obtained at HP of − 55 mV from those at HP − 80 mV (Fig. 1B-a, dashed line). We recorded only ICaL flowing through Cav1.3 and Cav1.2 isoforms in Cav3.1−/− SAN cells, whereas ICaT was undetectable (Fig. 1B-b). In Cav1.3−/− SAN cells, total ICa (black dots, Fig. 1B-c) was generated by Cav3.1-mediated ICaT and Cav1.2-mediated ICaL (red dots, Fig. 1B-c). We quantified ICaT by subtracting traces obtained at the two different HPs (Fig. 1B-c, dashed line). In Cav1.3−/−/Cav3.1−/− SAN cells the residual ICa recorded was Cav1.2-mediated ICaL, which was recognizable by its activation at positive membrane voltages, and faster inactivation kinetics than Cav1.3-mediated ICaL recorded in wild-type cells (Fig. 1B-d). No ICa could be recorded at voltages negative to − 35 mV in Cav1.3−/−/Cav3.1−/− SAN cells. A comparison between ICa densities recorded in SAN cells from wild-type and mutant animals is depicted in Fig. 1C (HP = − 80 mV) and Fig. 1D (HP = − 55 mV). In conclusion, concomitant ablation of SAN-VGCCs abolished diastolic ICa in Cav1.3−/−/Cav3.1−/− SAN cells.
Deep bradycardia and heart block in Cav1.3−/−/Cav3.1−/− mice
To evaluate SAN pacemaking and atrioventricular (AV) conduction, we recorded in vivo atrial rates (P–P interval) and ventricular rates (HRs) in mutant mice, under control conditions (Fig. 2A and Supplementary Fig. 1, ANS+) and following combined injection of atropine and propranolol (Fig. 2B, ANS−) to inhibit autonomic input. All mutant mice displayed significant slowing of atrial and ventricular rates: Cav3.1−/− (~ 10%), Cav1.3−/− (~ 30%) and Cav1.3−/−/Cav3.1−/− (~ 35%) in comparison to wild-type counterparts under control conditions (Fig. 2A,C,E and Supplementary Fig. 1). We recorded deep bradycardia in Cav1.3−/− and Cav1.3−/−/Cav3.1−/− mice under inhibition of autonomic nervous system (~ 310 bpm, Fig. 2B–D). The averaged atrial rates of Cav1.3−/− and Cav1.3−/−/Cav3.1−/− mice did not differ significantly, either under control conditions or following inhibition of autonomic nervous system input, suggesting that Cav3.1 channels may share a common downstream effector with Cav1.3 to generate pacemaking. Both Cav1.3−/− and Cav1.3−/−/Cav3.1−/− mice showed high incidence of 2nd degree AV block (Fig. 2A). None of the Cav1.3−/− mice studied presented with complete 3rd degree AV block (complete heart block). In contrast, 25% of Cav1.3−/−/Cav3.1−/− mice presented complete 3rd degree AV block (Fig. 2A), indicating additive effects of SAN-VGCCs ablation on AV conduction. Inhibition of the autonomic nervous system input reduced atrial rates in all genotypes tested and abolished 2nd and 3rd degree AV blocks in mutant mice (Fig. 2F). The AV conduction time (PR interval) was increasingly prolonged in mutant Cav3.1−/−, Cav1.3−/− and Cav1.3−/−/Cav3.1−/− mice (Supplementary Fig. 1). The uncorrected QT interval was also significantly prolonged in Cav1.3−/− and Cav1.3−/−/Cav3.1−/− mice (Supplementary Fig. 1). Taken together, these results indicate additive effects of Cav1.3 and Cav3.1 loss-of-function on AV conduction.
Rhythm dissociation and ventricular arrhythmia in Cav1.3−/−/Cav3.1−/− hearts
Because we observed complete 3rd degree AV block in Cav1.3−/−/Cav3.1−/− mice, we addressed the consequences of concomitant ablation of SAN-VGCCs on intrinsic heart automaticity, in the absence of autonomic input, in Langendorff-perfused hearts (Fig. 3). The spontaneous atrial rates of mutant hearts were lower than that of wild-type counterparts. Contrary to what we observed in vivo, all Cav1.3−/−/Cav3.1−/− hearts investigated displayed complete 3rd degree AV block and rhythm dissociation (Fig. 3A,B). Rhythm dissociation was evidenced by the difference between measured atrial and ventricular rates in Cav1.3−/− and Cav1.3−/−/Cav3.1−/− hearts (Fig. 3C). Furthermore, concomitant ablation of the two SAN-VGCCs was highly proarrhythmic. Indeed, Cav1.3−/−/Cav3.1−/− hearts showed atrial and ventricular escape rhythms, suggesting extranodal impulse generation, as well as episodes of ventricular tachycardia (Supplementary Fig. 2). Taken together, these observations indicated that concomitant ablation of SAN-VGCCs disrupted normal automaticity of intact hearts by impairing impulse generation in the SAN, as well as by inducing complete block of AV conduction and ventricular arrhythmia.
Concomitant ablation of SAN-VGCCs disrupts SAN automaticity and shifts the pacemaker leading sites to peripheral SAN or to extranodal locations
Since we recorded limited residual SAN driven heart automaticity in isolated Cav1.3−/−/Cav3.1−/− hearts we attempted to study SAN impulse formation and localization using optical mapping (OM) of membrane voltage in isolated SAN/atria preparations. All wild-type SAN/atria preparations consistently displayed the pacemaker leading site within a limited region of the central cranial side of the SAN at the entry of superior vena cava in the right atrium and close to the border of the Crista Terminalis (Fig. 4A). In contrast, we found that automaticity in Cav3.1−/− and Cav1.3−/− SAN/atria preparations was characterized by the presence of at least two leading sites with alternating dominance in 5 out of 8 and in 4 out of 7 tissues tested, respectively (Fig. 4A). In addition, 2 out of 7 Cav1.3−/− SAN/atria preparations showed 3 alternating leading sites. Finally, all Cav1.3−/−/Cav3.1−/− preparations studied displayed alternating automaticity between two or three leading sites (Fig. 4A). In Cav3.1−/− preparations, secondary leading sites appeared in the correspondence of the nodal extension caudally to the inferior vena cava31. In Cav1.3−/− and Cav1.3−/−/Cav3.1−/− SAN/atria we observed secondary leading sites also in the right or left atrium (Fig. 4A). Emergence of multiple extranodal leading sites was accompanied by increasingly slow automaticity in Cav3.1−/−, Cav1.3−/− and Cav1.3−/−/Cav3.1−/− SAN/atria preparations (Fig. 4B). Consistent with slowing of automaticity, the coefficient of variability of atrial rate also increased with concomitant ablation of SAN-VGCCs in comparison to wild-type counterparts (Fig. 4C). The rate of atrial impulse was negatively correlated with the distance between the alternating leading sites, suggesting increasing dominance of peripheral SAN or extranodal sites in Cav1.3−/−/Cav3.1−/− SAN/atria preparations (Fig. 4D). Taken together, these observations indicate that concomitant ablation of SAN-VGCCs disrupted primary SAN automaticity and shifted pacemaker activity to peripheral SAN or to extranodal sites resulting in slow atrial rates.
If and INaTTX sustain automaticity in Cav1.3−/− and Cav1.3−/−/Cav3.1−/− mice in vivo
Since we observed residual automaticity in hearts of Cav1.3−/− and Cav1.3−/−/Cav3.1−/−, originating either in the peripheral SAN or in extranodal regions (Fig. 4), we attempted to identify the mechanisms underlying residual pacemaking. f-(HCN) channels underlie cardiac If32,33. Because of their direct sensitivity to intracellular cAMP, f-channels are targets of opposite regulation of HR by catecholamines and acetylcholine2. We thus hypothesized that the decrease in HR observed in Cav1.3−/− and Cav1.3−/−/Cav3.1−/− mice following inhibition of the autonomic nervous system was generated by concomitant atropine induced IKACh inhibition34,35 and propranolol induced shift of If activation to more negative voltages (Fig. 2B). We thus administered selective If blocker ivabradine (6 mg/kg) to wild-type and mutant mice and recorded HR under these conditions (Fig. 5). Ivabradine decreased the HR in all genotypes and reduced atrioventricular blocks in in Cav1.3−/− and Cav1.3−/−/Cav3.1−/− mice (Fig. 5A–D). The HR recorded in Cav1.3−/−/Cav3.1−/− mice under ivabradine administration was similar to that observed in the same mice under atropine and propranolol, suggesting that If was contributing to set the HR in Cav1.3−/− and Cav1.3−/−/Cav3.1−/− mice in vivo under the action of the autonomic nervous system. We did not observe episodes of spontaneous atrial or ventricular arrhythmia in wild-type or mutant mice upon administration of ivabradine. The difference in HR before and after ivabradine administration was similar among wild-type and Cav3.1−/− mice, but was increased in Cav1.3−/− and Cav1.3−/−/Cav3.1−/−mice (Fig. 5D), showing that individual deletion of Cav1.3 or concomitant ablation of SAN-VGCCs increased the relative importance of If to residual HR.
We then investigated the origin of the residual SAN automaticity following inhibition of If under ex vivo conditions, to prevent potentially lethal bradycardia in vivo. Similarly to in vivo conditions, ivabradine (10 µM) induced low HRs in isolated hearts from mutant Cav1.3−/− and Cav1.3−/−/Cav3.1−/− mice (< 100 bpm; Fig. 6A). Previous work showed that INa(TTX) contributes to the basal HR and to SAN pacemaker activity in mammals8,9,10,11. We thus employed a moderate concentration of TTX (100 nM) to investigate the contribution of this current to the residual automaticity of Cav1.3−/− and Cav1.3−/−/Cav3.1−/− hearts under conditions of If block by ivabradine. TTX further reduced HR in Cav1.3−/− and Cav1.3−/−/Cav3.1−/− hearts to very low levels (< 50 bpm; Fig. 6B). Since part of this residual automaticity could come from impulse generation in extranodal sites (Fig. 5), we directly investigated SAN automaticity by OM of the pacemaker impulse following pharmacologic inhibition of If (Fig. 6C), or concomitant If and INa(TTX) blockade (Fig. 6D). Blockade of INa(TTX) in SANs perfused with ivabradine arrested impulse generation in 4/6 Cav1.3−/− and 3/6 Cav1.3−/−/Cav3.1−/− preparations (Fig. 6D). Very low rates of spontaneous depolarization were recorded in 5 SAN/Atria preparations from Cav1.3−/− and Cav1.3−/−/Cav3.1−/− mice still presenting residual automaticity (< 40 bpm, Fig. 6D). In contrast, ablation of SAN-VGCCs did not affect the impulse conduction time to the atria (Supplementary Fig. 3). Similarly, ivabradine and TTX did not prolong the impulse conduction times to the right and left atria (Supplementary Fig. 3). These data indicate that while SAN-VGCCs strongly affected SAN automaticity, they were not directly involved in impulse conduction from the SAN to atria.
Concomitant ablation of SAN-VGCCs impaired the generation of SAN impulse in the SAN (Figs. 3, 4). Hence, we recorded automaticity of isolated SAN cells from wild-type and mutant mice (Fig. 7). Individual Cav1.3 knockout and concomitant ablation of the two SAN-VGCCs similarly slowed basal automaticity of isolated SAN cells and reduced the slopes of linear and exponential phases of the diastolic depolarization (Fig. 7A–D, Supplementary Table 1). In addition, individual Cav1.3 or concomitant SAN-VGCCs ablation hyperpolarized the action potential threshold to more negative voltages in comparison to wild-type and Cav3.1−/− SAN cells, without affecting the cells’ maximum diastolic potential (Supplementary Table 1). Superfusion of ivabradine alone significantly slowed automaticity of SAN cells isolated from all genotypes (Fig. 7A–D). Ivabradine also significantly hyperpolarized the maximum diastolic potential in SAN cells (Supplementary Table 2), indicating that If contributed to set the equilibrium between inward and outward currents at the end of the repolarization phase. Concomitant perfusion of ivabradine and TTX further slowed automaticity in comparison to ivabradine alone in SAN cells from all genotypes (Fig. 7A–D). Concomitant perfusion of ivabradine and TTX slowed but did not arrest automaticity of wild-type or Cav3.1−/− SAN cells (Fig. 7A,B). In contrast with Cav3.1−/− SAN cells and consistently with OM recordings, concomitant inhibition of If and INa(TTX) slowed the rate of spontaneous action potentials by ~ 80% and arrested automaticity in 2 out of 9 of Cav1.3−/− and in 3 out of 9 Cav1.3−/−/Cav3.1−/− isolated SAN cells (Fig. 7C,D). We tested also if concomitant selective pharmacologic inhibition of SAN-VGCCs affected automaticity of SAN cells similarly to genetic ablation. To obtain selective inhibition of Cav1.3 channels, we employed SAN cells from mice carrying dihydropyridine (DHP) resistant Cav1.2 channels (Cav1.2DHP−/−, see Ref.36). In Cav1.2DHP−/− mice, Cav1.2 channels have been rendered insensitive to DHP by knock-in a point mutation in the channel DHP binding site36. Concomitant perfusion of the T-type selective inhibitor TTA-A2 and nifedipine slowed the rate of spontaneous action potentials by ~ 90% (Supplementary Fig. 4 and Supplementary Table 3). These data indicate that the strong reduction of SAN automaticity following ablation of SAN-VGCCs could not be explained by a remodeling phenomenon of the pacemaker mechanism in mice globally lacking SAN-VGCCs.
Concomitant ablation of SAN-VGCCs reduced late diastolic local Ca2+ release linked to the coupled clock pacemaker mechanism
Experiments on SAN/Atria preparations and recordings of isolated SAN cells from Cav1.3−/− and Cav1.3−/−/Cav3.1−/− mice under concomitant inhibition of If and INa(TTX) showed arrest of automaticity. This observation suggests that, following ablation of SAN-VGCCs, functional association between RyR-dependent LCRs underlying the Ca2+ clock and L-type Cav1.2 channels could not consistently generate residual pacemaking under these conditions. We previously showed that Cav1.3 channels positively regulate the frequency and synchrony of LCRs during pacemaking27. Therefore, we recorded RyR-dependent Ca2+ release and [Ca2+]i dynamics in wild-type and mutant SAN cells to investigate the consequences of SAN-VGCCs ablation on late diastolic LCRs, which constitute one hallmark of the coupled clock pacemaker mechanism15. In line with recordings of spontaneous action potentials, the frequency of spontaneous [Ca2+]i transients was similar in Cav1.3−/− and Cav1.3−/−/Cav3.1−/− SAN cells, but lower than in wild-type and Cav3.1−/− counterparts (Fig. 8A–D). Concomitant blockade of If and INa(TTX) reduced the frequency of spontaneous [Ca2+]i transients by 62% in Cav1.3−/− and by 74% in Cav1.3−/−/Cav3.1−/− SAN cells, leading to very low averaged rates of automaticity (< 15 bpm; Fig. 8C,D).
We then analyzed LCRs events occurring in the late diastolic interval3. These late diastolic LCRs generate an ascending phase (ramp) of the average fluorescence ratio (F/F0) signal (Fig. 9A)27,37. We observed reduced events of late diastolic LCRs in Cav1.3−/− and Cav1.3−/−/Cav3.1−/− SAN cells (Fig. 9A). The reduction in LCRs was reflected in diminished time integral of the ramp phase in comparison to wild-type cells (Fig. 9B). The slope of the ramp phase is indicative of the degree of synchronization of late LCRs and constitute an important parameter of the capability of the coupled clock to promote diastolic NCX1 activity hence pacemaking15,16,38. The slope of the ramp phase was strongly decreased in Cav1.3−/− and Cav1.3−/−/Cav3.1−/− SAN cells (Fig. 9C). We did not record significant differences in the time integral or ramp slope between Cav1.3−/− and Cav1.3−/−/Cav3.1−/− SAN cells. In conclusion, these observations indicated that concomitant ablation of SAN-VGCCs disrupted late diastolic LCRs, which constitute an important component of the coupled clock pacemaker mechanism.
We previously showed that ablation of Cav1.3 channels preserves the SR Ca2+ load27. Therefore, we challenged wild-type and mutant SAN cells by rapid dumping of [Ca2+]i using caffeine (10 mM). The caffeine-evoked [Ca2+]i transient of wild-type and Cav3.1−/− SAN cells showed comparable amplitudes (F/F0, Supplementary Fig. 5A). In contrast, Cav1.3−/− and Cav1.3−/−/Cav3.1−/− SAN cells showed increased F/F0 value in comparison to wild-type counterparts (Supplementary Fig. 5A). The decay time (τ) of the caffeine-evoked [Ca2+]i transient did not differ between wild-type and mutant SAN cells, indicating similar activities of SERCA and NCX in all genotypes tested (Supplementary Fig. 5B). Finally, we compared the total number of LCRs, irrespectively from their relative position in diastolic interval, as we described previously27. We did not record significant differences in the total number of diastolic LCRs under perfusion of ivabradine (3 µM) or concomitant perfusion of ivabradine (3 µM) and TTX (50 nM) in comparison to control conditions in all the genotypes tested (Supplementary Fig. 5C), indicating that inhibition of If or INa(TTX) did not alter the function of RyRs. Taken together, these results show that concomitant ablation of SAN-VGCCs disrupts late diastolic LCRs underlying the SAN coupled clock mechanism of pacemaking.
Impact of the study
In this study, we show for the first time that concomitant ablation of SAN-VGCCs disrupts heart automaticity and suppresses late diastolic LCRs, which are an important factor of the coupled clock pacemaker mechanism in SAN cells. SAN-VGCCs ablation compromised SAN impulse generation, impaired atrioventricular conduction and destabilized ventricular rhythmicity. Particularly, we show that Cav3.1 channels play an important role in stabilizing atrial and ventricular rhythmicity in isolated Cav1.3−/− hearts. We also report that in both Cav1.3−/− and Cav1.3−/−/Cav3.1−/− SAN/atria preparations, residual pacemaker activity is predominantly generated in peripheral nodal and extranodal sites by f-channels and TTX-sensitive Na+ channels.
The physiological role of SAN-VGCCs in orchestrating heart automaticity has been long overlooked. Therefore, designs of many currently published studies and review articles on physiopathology of heart automaticity do not still properly factor SAN-VGCCs. The original formulation of the coupled clock model of pacemaking ascribed predominant roles for f-channels and spontaneous voltage-independent diastolic LCRs, respectively as key components of the membrane clock and of the Ca2+ clock in the generation of the diastolic depolarization3,5,15. In this model, cooperation between f-channels and NCX1 was the dominant electrogenic mechanism in the generation of diastolic depolarization. The contribution of ICaT was predicted to be reduced in comparison to that of If and NCX115. Another important factor in the coupled clock is high basal intracellular cAMP concentrations and elevated activity of protein kinases that contribute to mutual entrainment between diastolic RyR-dependent Ca2+ release underlying the Ca2+ clock and the membrane clock. The earlier coupled clock model by Maltsev and Lakatta15 also included activation of ICaL at positive voltages corresponding to the action potential threshold15. Thus, while ICaL did not directly contribute to the generation of diastolic depolarization per se, Ca2+ entry via ICaL during the action potential phase was necessary to restore SR Ca2+ load to prime a new cycle of diastolic LCRs15. However, this coupled clock model did not include evidence showing that SAN cells express both Cav1.2-mediated ICaL, activating at positive systolic voltages, as well as Cav1.3-mediated ICaL, activating at diastolic voltages and supplying Ca2+ entry during diastolic depolarization24. In addition, further work from our group showed that Ca2+ entry via Cav1.3 channels control RyR-dependent Ca2+ release by triggering and synchronizing diastolic LCRs events27. The most recent version of the coupled clock model of pacemaking by Lyaskov et al.16 includes a more negative activation threshold for activation of ICaL16 (− 40 mV, see Ref.39), than in the previous version of the model15. Numerical simulations using the Lyaskov et al. coupled clock model16 predict that this negative shift of ICaL activation induces functional cooperativity, between LCRs, NCX1 and ICaL to generate exponential phase of diastolic depolarization (referred to as “ignition phase”). The present study is consistent with this model. However, and importantly for the current models of pacemaking, we now provide direct genetic and functional evidence showing that the mechanism of SAN dysfunction following ablation of SAN-VGCCs involves suppression of Ca2+ entry in the voltage range spanning the diastolic depolarization and impairment of generation of late diastolic LCRs. Thus, SAN-VGCCs provides an important regulatory mechanism in sustaining the Ca2+ clock during normal pacemaking by promoting the coupled clock. In addition, our study shows that coupling between Cav1.2 and random LCRs generated by RyR-dependent Ca2+ release cannot consistently generate viable SAN automaticity following ablation of SAN-VGCCs and inhibition of f-channels. In this context, our data indicate that future models of pacemaking should include Cav1.3 and Cav3.1 channels as additional mechanisms of coupling between the Ca2+ clock and the membrane clock, and distinguish between functional roles of Cav1.3 from Cav1.2 channel isoforms.
Role of SAN-VGCCs in Ca2+ entry in SAN diastolic depolarization
Concomitant ablation of SAN-VGCCs abolished ICa at voltages spanning the range between ~ − 60 mV and ~ − 35 mV corresponding to diastolic depolarization in wild-type cells (Fig. 1, Supplementary Table 1). Previous work showed that Cav3.1 channels activate at more negative voltages than Cav1.3 channels7,24. However, their limited steady-state availability at the maximum diastolic potential (~ 90% steady-state inactivation at − 60 mV)24 predicts smaller Cav3.1-mediated ICaT than Cav1.3-mediated ICaL23. This suggests more limited contribution of Cav3.1-mediated ICaT to SAN diastolic depolarization than of Cav1.3-mediated ICaL. Furthermore, beside the higher steady-state availability of Cav1.3 than Cav3.1 at diastolic voltages, Cav1.3 channels are also an essential molecular component of the sustained inward Na+ current (Ist)40, which constitute an additional contributor to diastolic depolarization41. It is thus possible that, while Cav1.3 channels contribute to the pacemaker mechanisms via ICaL/Ist inward currents24,40 and generation of LCRs (Ref.35 and this study), the predominant contribution of Cav3.1 channels to SAN pacemaking is regulation of LCRs connected to the coupled clock mechanism. In line with this hypothesis, we did not observe additivity between individual ablation of Cav1.3 and concomitant ablation of SAN-VGCCs on atrial rate in vivo (Fig. 2), as well as in Langendorff-perfused intact hearts (Fig. 3). Thus, another important factor determining similar HRs in Cav1.3−/− and Cav1.3−/−/Cav3.1−/− mice is the convergence of SAN-VGCCs to a common downstream intracellular effector: the generation of diastolic LCRs (Fig. 9). This hypothesis is also consistent with previous evidence on the role of Cav3.1-mediated ICaT in generating diastolic LCRs in atrial subsidiary pacemaker myocytes37. However, isolated SAN/atria from Cav1.3−/−/Cav3.1−/− hearts showed significantly slower intrinsic automaticity than that of Cav1.3−/− counterparts (Fig. 4). The discrepancy between the additivity of the effect of SAN-VGCCs in isolated Cav1.3−/−/Cav3.1−/− hearts and SAN/atria preparations and the observations in vivo could be explained by the action of the autonomic nervous system and by physiological mechanoelectrical feedback stimulating pacemaking in working hearts in vivo.
Role of SAN-VGCCs in SAN automaticity
The observation that Cav1.3−/−/Cav3.1−/− hearts ex vivo present with junctional atrial and ventricular rhythmicity rather than SAN rhythm led us to investigate automaticity using isolated SAN/atria preparations. Both and Cav3.1−/− and Cav1.3−/− presented with shift of the leading pacemaker sites from central to peripheral SAN areas (Fig. 4). Pacemaker shift is a well-known phenomenon in which groups of automatic cells eventually become dominant following manoeuvres that reduce intrinsic spontaneous activity of previously dominant leading sites (see Ref.42, for review). Shift is due to heterogeneity of expression of ion channels42 and proteins regulating the Ca2+ clock in the SAN43,44. Recent work has shown that normal SAN impulse is initiated in the leading site by HCN4-expressing cells showing heterogeneous Ca2+ signals45. Indeed, these cells present with spontaneous LCRs, or LCRs followed by cell-wide Ca2+ transients. These cells can entrain the activity of neighbouring cells or cluster of cells. Different clusters can synchronize their activity to promote the emergence of the SAN impulse45. It is thus tempting to speculate that deletion of SAN-VGCCs disrupts LCRs in these clusters shifting the pacemaker leading site towards peripheral or extranodal regions that are less sensitive to Cav1.3 and Cav3.1 deletion and LCRs suppression. These secondary pacemaker regions, such as the peripheral SAN or the posterior nodal extension close to the inferior vena cava31 express high levels of the f-channel protein HCN4, which would constitute an effective backup pacemaker mechanism. This hypothesis is consistent with our observation that, in comparison with Cav1.3−/−, Cav1.3−/−/Cav3.1−/− SAN-atria preparations more frequently showed alternating impulse initiation in the SAN and in extranodal sites (Fig. 4), and with our previous observations that pacemaker cells of the mouse peripheral SAN show strong HCN4 positive immunostaining34. In addition, both cardiac Nav1.5 and TTX-sensitive Nav isoforms have been shown to participate to pacemaking in the peripheral SAN and to intranodal conduction in mice8 and humans10. It is thus possible that peripheral leading sites express elevated densities of INa necessary for impulse conduction within the SAN or from the SAN to the atria10. Consistent with this hypothesis, ablation of SAN-VGCCs did not affect the SAN-to-atria conduction time (Supplementary Fig. 3). Shift of pacemaker site to peripheral SAN could explain why concomitant inhibition of If and TTX-sensitive INa arrests automaticity in the majority of Cav1.3−/− and Cav1.3−/−/Cav3.1−/− SAN-atria preparations (Fig. 6).
In conclusion, our data show for the first time that SAN-VGCCs are necessary to maintain atrial automaticity in the central SAN region. Concomitant ablation of SAN-VGCCs reduced the HR in vivo by ~ 200 bpm, which corresponds to ~ 30% of the HR measured in wild-type mice under control conditions (Fig. 2). This value is similar to that recorded in wild-type mice under If inhibition by ivabradine (~ 200 bpm). However, the HR in some Cav1.3−/−/Cav3.1−/− mice under If inhibition was lower than 100 bpm, which corresponds to a reduction of more than 85%, in comparison to the initial HR measured in wild-type mice under control condition. Nevertheless, this residual pacemaking was sufficient to sustain heart function in mice. These data underscore the importance of SAN-VGCCs in the generation of SAN automaticity.
Role of SAN-VGCCs in impulse conduction and ventricular rhythmicity
We found a clearly additive impact on AV conduction between individual Cav1.3 and concomitant SAN-VGCCs ablation (Figs. 2, 3). More hyperpolarized diastolic voltages in some AVN cells and in myocytes of His’–Purkinje system than in the SAN could explain this additive effect. This would increase the steady-state availability of Cav3.1-mediated ICaT in the conduction system. Consequently, dissociated rhythms observed in Cav1.3−/−/Cav3.1−/− mice can be explained also by loss of depolarization reserve provided by ICaT in Cav1.3−/− mice. The high incidence of complete heart block with dissociated atrial and ventricular rhythms in Cav1.3−/−/Cav3.1−/− hearts underscores the importance of Cav3.1 channels in atrioventricular conduction (Fig. 3). Shifting of leading pacemaker sites from primary sites to extranodal locations can explain atrial ectopic and atrial rhythmicity observed in Langendorff-perfused Cav1.3−/−/Cav3.1−/− hearts (Supplementary Fig. 2). This impairment of primary SAN automaticity by ablation of SAN-VGCCs could promote ventricular arrhythmia by unmasking the activity of automatic junctional proarrhythmic sites. In particular, complete heart block in Cav1.3−/−/Cav3.1−/− hearts may favor ventricular arrhythmia by unmasking junctional or ventricular ectopic sites. Indeed, we showed previously that episodes of SAN pauses or AV block precede spontaneous initiation of ventricular tachycardia in mice carrying heart specific expression of non-conductive f-channels (HCN4-AYA mice)34. A compensatory effect by autonomic tone may explain the absence of ventricular arrhythmia in Cav1.3−/−/Cav3.1−/− mice in vivo. In this respect, we showed that atropine suppressed ventricular tachycardia in HCN4-AYA mice34. Thus, blockade of IKACh activation by atropine could be an additional factor suppressing ventricular arrhythmia in Cav1.3−/−/Cav3.1−/− mice in vivo under inhibition of the autonomic nervous system (Fig. 1). In conclusion, our data show that expression of Cav3.1 channels is essential to maintain normal atrial and ventricular rhythmicity in hearts lacking Cav1.3.
Role of SAN-VGCCs in the pacemaker mechanism
Beside SAN-VGCCs and If, other plasmalemmal ion channels, as well as RyR-dependent Ca2+ release can contribute to the generation of diastolic depolarization and pacemaker activity. We thus hypothesized that the residual pacemaker activity observed in vivo in Cav1.3−/− and Cav1.3−/−/Cav3.1−/− mice or in ex vivo mutant hearts and SAN/atrial preparations under ivabradine was sustained by TTX-sensitive Nav1 channels and RyR-dependent Ca2+ release.
Previous work has showed that If inhibition by ivabradine reduces diastolic LCRs, by slowing the coupled clock in a rate-dependent way46. In particular, the coupled clock model of pacemaking predicts that If inhibition will slow pacemaker activity, which in turn, will reduce LCRs via the coupled clock mechanism16,46. In relation to the present study, it is possible that slowing of diastolic depolarization by If inhibition delays activation of SAN-VGCCs contributing to reduction in diastolic LCRs in wild-type SAN cells. However, our data show that deletion of SAN-VGCCs leads already to strong reduction in late diastolic LCRs with consequent disruption of the coupled clock mechanism (Fig. 9). The evidence that inhibition of If and INa(TTX) does not reduce the total LCRs number in mutant cells despite further slowing of pacemaking is consistent with our hypothesis that ablation of SAN-VGCCs disrupts the normal functioning of the coupled clock. Consequently, it is possible that the role of If in residual pacemaker activity observed following concomitant ablation of SAN-VGCCs differs from that observed in wild-type cells, in which the coupled clock mechanism is intact before If blockade. This hypothesis is in line with our observation that the relative heart rate slowing induced by If inhibition is higher in Cav1.3−/− and Cav1.3−/−/Cav3.1−/− than in wild-type mice (Fig. 5). This differential contribution of If to pacemaking in wild-type and mutant hearts underscores the importance of the depolarization reserve in the intact SAN, in which If, beside contributing to cellular automaticity, prevents tissue hyperpolarization imposed by atrial electrotonic load6,47.
Our data show that inhibition of INa(TTX) arrests automaticity in 50% of isolated SAN/atria preparations and in 40% individual Cav1.3−/− and Cav1.3−/−/Cav3.1−/− SAN cells. This observation indicates that TTX-sensitive Nav1 channels are the predominant mechanisms sustaining pacemaker activity after ablation of SAN-VGCCs and pharmacologic inhibition of If (Figs. 6, 7). We may expect that spontaneous RyR-dependent Ca2+ release could remain viable under these conditions, as predicted by the coupled clock model3. Indeed, Cav1.3−/− and Cav1.3−/−/Cav3.1−/− hearts express functional Cav1.2 channels and could ensure proper SR Ca2+ load to activate spontaneous Ca2+ clock mechanism3. This hypothesis would be in line with our observation that deletion of Cav1.3 channels does not reduce the SR Ca2+ load27. However, our data show that SAN-VGCCs are a key factor to generate and control diastolic LCRs underlying the coupled clock mechanism of pacemaking. Several combined factors may explain the poor efficiency of Cav1.2 channels in maintaining proper pacemaking, when functionally associated with If and spontaneous RyR-dependent Ca2+ release. Beside the more positive threshold for activation of Cav1.2 in comparison to Cav1.3 channels, Cav1.3-mediated ICaL activation kinetics is faster and inactivation is lower than that of Cav1.2-mediated ICaL in SAN cells24. Differences in channels’ kinetics probably render Cav1.3 more adaptable to generation of rhythmic activity in SAN cells than Cav1.2 channels. Moreover, the membrane distribution of Cav1.2 channels differs from that of Cav1.3 in mouse SAN cells. Indeed, we showed previously that while Cav1.2 immunoreactivity is evenly distributed along the plasma membrane, Cav1.3 immunoreactivity shows well-defined co-staining with RyR248. This differential subcellular distribution of Cav1.3 channels may facilitate control of LCRs in the diastolic phase in comparison to Cav1.2 channels that reside in a less-favorable spatial position to generate LCRs. In line with this hypothesis, we previously showed that ablation of Cav1.3 channels abolishes synchronous recruitment of diastolic RyR-dependent Ca2+ releasing sites under adrenergic activation of pacemaker activity27. Loss of synchronous recruitment intervenes despite the significant negative shift in the threshold for activation of Cav1.2-mediated ICaL induced by adrenergic activation (~ 5 mV)27.
It is possible that residual RyR-dependent Ca2+ release was effective in the fraction of SAN/atria preparations still generating automaticity at very low rates, probably in cooperation with TRPC14 and TRPM412 channels. Nevertheless, our results show that expression of SAN-VGCCs is necessary to ensure pacemaking, via Ca2+ entry and efficient generation of diastolic LCRs in diastolic depolarization; two functions that sole expression of Cav1.2 channels could not facilitate.
Our study demonstrates that co-expression of functional Cav1.3 and Cav3.1 SAN-VGCCs is essential for generating normal heart automaticity and atrioventricular conduction, as well as to stabilize atrial and ventricular rhythmicity and generating SAN pacemaker activity. We show that normal heart automaticity is reliant on expression of SAN-VGCCs to generate diastolic inward current and Ca2+ entry to activate the Ca2+ clock pacemaker mechanism during normal pacemaking. As a corollary, our study indicates that functional association of L-type Cav1.2 channels and f-channels is not sufficient to maintain normal SAN pacemaking and heart automaticity. Finally, our study provides new mechanistic insight into congenital and autoimmune SAN dysfunction and atrioventricular block based on SAN-VGCC loss-of-function29,30,49.
Mutant mice harboring genetic ablation of VGCCs (Cav3.1−/−, Cav1.3−/− and Cav1.3−/−/Cav3.1−/−) were generated in the IGF animal facility from C57B/6J genetic background. Cav1.3−/−/Cav3.1−/− mice were obtained by crossing C57B/6J Cav1.3−/− 27 with C57B/6J Cav3.1−/−25 mice. The investigation conforms to the Guide for the Care and Use of Laboratory Animals published by the US national Institute of Health (NIH Publication No. 85–23, revised 1996) and European directives (2010/63/EU). The experimental procedure was approved by the Ethical committee of the University of Montpellier and the French Ministry of agriculture (protocol no: 2017010310594939). Animals were housed in individual cages with free access to food and water and were exposed to 12-h light/dark reverse cycles (light, 20:00 h to 8:00 h) in a thermostatically controlled room.
ECG recordings in mice
Telemetric ECG recordings in freely-moving mice were performed as previously described23,34,35. Briefly, mice were anesthetized using gas anesthesia with 2% isoflurane (Forene, Abbott, UK). An ETA-F10 telemetric transmitter (Data Sciences International) was placed subcutaneously along the animal’s back. Transmitter’s wire electrodes were placed in DII derivation against the heart axis. Advil (ibuprofen, 7 mL/L) was added to the drinking water for 4 days after implantation to prevent post-operative pain. Mice were left to recover for 10 days before experiments. ECG signals were recorded by employing a telemetric receiver connected to an analog-to-digital conversion acquisition system. Analysis was performed off-line using the Dataquest A.R.T. software (Data Sciences International). Heart rates (HR) were determined from RR intervals and atrial rates from PP intervals, under control conditions or following recording of 4-h baseline to evaluate drugs effects. All drugs were administered by intraperitoneal (IP) injection. Mean HR values were calculated by analyzing periods of 5 min at different time points corresponding to the peak effect of the drug. ECG parameters were measured by employing ECG Auto 1.5.7 software (EMKA Technologies).
ECG recordings in Langendorff perfused mouse hearts were performed as previously described35. Mice were deeply anesthetized by IP injection of 0.3 mL of solution constituting Ketamine (0.1 mg/g, Imalgène) and Xylazine (0.01 mg/g, Rompun 2%, Bayer), followed by a second injection of Pentobarbital (150 µL Euthasol Vet in 10 mL NaCl physiological solution). To avoid blood clots in the heart, we injected 0.5 mL of NaCl solution containing Heparin (25,000 U.I). Hearts were removed via thoracotomy when the tail sensitivity test become negatives. Excised hearts with aortic cannula were quickly mounted on a Langendorff apparatus (Isolated heart system; EMKA Technologies) at a pressure of 70–80 mm Hg imposed by the working heart with normal Tyrode’s solution containing (mM): NaCl, 140; KCl, 5.4; MgCl2, 1; CaCl2, 1.8; Hepes, 5 and glucose, 5.5 (pH adjusted to 7.4 with NaOH). Perfused hearts were immersed in the water-jacked bath and maintained at 36 °C. The ECG was continuously recorded by two Ag–AgCl electrodes; the first was placed on the epicardial side of the right atrium close to the SAN area and the second near the ventricular apex. The heart rate was allowed to stabilize for at least 30 min before perfusion of drugs. ECG parameters were measured with ECG Auto 184.108.40.206 software (EMKA Technologies).
Intact SAN/atria preparations
We obtained SAN-atria preparations from excised hearts as previously described50. Briefly, we placed the entire SAN/atrial preparation in pre-warmed (36 °C) Tyrode’s solution containing heparin (10 U/mL). We used a stereomicroscope (SZX16; Olympus) with low magnification (7 ×) to transilluminate and visualize directly the isolated SAN/atria preparation. We identified the SAN region using the superior and inferior vena cava, the crista terminalis, and the interatrial septum as landmarks. The SAN/atrial preparation including right and left atria (RA and LA) was pinned to the bottom of an optical chamber (Fluorodish, FD35PDL-100; WPI) coated with ~ 2 mm of clear Sylgard (Sylgard 184 Silicone elastomer kit; Dow Corning). To maintain the SAN in a flat plane, we pinned the right and left atrium using entomology needles.
Optical mapping of membrane voltage in SAN/atria preparations
Optical mapping of SAN impulse was performed as described previously51. SAN/atria preparation was loaded with the voltage-sensitive dye indicator Di-4-ANEPPS in Tyrode’s solution with 10 μM Biotium for at least 30 min at room temperature (20–22 °C). The preparation was placed on an agitated plate to maintain proper oxygenation of the tissue and load it uniformly. The tissue was then washed 3 times in dye-free Tyrode’s solution. The SAN/atria preparation was continously perfused at 34–36 °C. A MICAM Ultima-L complementary metal oxide semiconductor (CMOS) camera (100 × 100-pixel CMOS sensor, 10 × 10 mm, SciMedia) was used to achieve high speed optical mapping of membrane voltage (2 ms/frame). The camera was mounted on a THT microscope, with two objectives (2 × and 1.6 ×) to generate a field view of 12.5 × 12.5 mm. The excitation light source consisted in a 150-W halogen light system with built-in shutter (SciMedia). The filter set included a 531/50-nm excitation filter, 580-nm dichroic mirror, and 580 long-pass emission filter. Blebbistatin (1.5–5 μM; Tocris Bioscience) was used to prevent imaging artifacts due to tissue contraction. We usually limited our recording times to 32.768 s (16,384 frames at 2 ms per frame) to preserve the tissue from dye phototoxicity. Data were analyzed using the BrainVision Analyzer software (BrainVision).
Isolation of SAN cells
SAN pacemaker cells were isolated as previously described52. Briefly, SAN tissue was immersed into solution containing 140 mM NaCl, 5.4 mM KCl, 0.5 mM MgCl2, 0.2 mM CaCl2, 1.2 mM KH2PO4, 50 mM taurine, 5.5 mM D-glucose, 1 mg/mL BSA, and 5 mM Hepes–NaOH (adjusted to pH 6.9 with NaOH) for 4 min. The tissue was then transferred into the low-Ca2+ solution containing purified collagenase and protease (Liberase medium Thermolysin concentration; 229 U/mL; Roche). 1.9 U/mL elastase (Boehringer Mannheim). Digestion was carried out for 15–20 min at 36 °C. To stop the digestion process, the SAN was washed in a medium containing 70 mM l-glutamic acid, 20 mM KCl, 80 mM KOH, 10 mM KH2PO4, 10 mM taurine, 1 mg/mL BSA, and 10 mM Hepes–KOH (adjusted to pH 7.4 with KOH). Single cells were isolated from the SAN tissue by manual agitation using a flame-forged Pasteur’s pipette in KB solution at 36 °C. To recover the automaticity of the SAN cells, Ca2+ was gradually reintroduced in the cells’ storage solution to a final concentration of 1.8 mM52.
Patch-clamp recordings of SAN cells
Patch-clamp recordings of SAN cells was carried out as previously described52. Cells were harvested in custom-made chambers with glass bottoms for cell attachment. Cells were then superfused with Tyrode’s solution warmed at 36 °C before recording. Voltage-gated Ca2+ currents (ICa) were recorded using standard whole cell patch-clamp configuration as previously described23,24. Extracellular recording solution contained (mM): 135 tetraethylammonium chloride (TEA-Cl), 10 4-aminopyridine (4-AP), 1 MgCl2, 0.03 tetrodotoxin (TTX), 1 g/L Glucose, 2 CaCl2, 10 Hepes, (adjusted to pH = 7.2 with TEAOH). Electrodes had a resistance of 3 MΩ and were filled with a solution containing (in mM): 125 Cs+-aspartate, 20 TEA-Cl, 1.2 CaCl2, 5 Mg-ATP, 0.1 Li2-GTP, 5 EGTA, and 10 HEPES (pH adjusted to 7.2 with CsOH)23,24. Seal resistances were in the range of 2–5 GΩ. Pacemaker activity of SAN cells was recorded under perforated patch conditions by adding 50 μM β-Escin to the pipette intracellular solution23,35. Patch-clamp electrodes had a resistance of 3–5 MΩ when filled with an intracellular solution containing (in mM): 130 K+-aspartate, 10.0 NaCl; 2 ATP-Na+ salt, 6.6 creatine phosphate, 0.1 GTP-Mg2+, 0.04 CaCl2 (pCa = 7.0), and 10.0 HEPES–KOH (adjusted to pH 7.2 with KOH). Perfusion of pre-warmed (36 °C) experimental solutions was performed by using a multi-MPRE8 heating pen (Cell Micro Controls,). Data acquisition was performed using a Multiclamp 700A patch-clamp amplifier connected to Digidata 1550B interface (Molecular Devices).
Ca2+ imaging in isolated SAN cells
Confocal Ca2+ imaging of SAN cells was performed as previously described27. SAN cells were harvested in a glass Fluorodish (FD3510-100, WPI), coated overnight with laminin (1 mg/mL; Sigma-Aldrich) for 1 h before recordings. SAN cells loading was performed by removing the bath solution and replacing it by Tyrode’s solution containing the Ca2+ indicator CAL-520 (1 µM, from a stock solution containing DMSO/Pluronic F-127 0.13%; Invitrogen) during 25 min at room temperature. Images were obtained with confocal microscopy (Zeiss LSM 780), by scanning SAN cells with an Argon laser in line-scan configuration. To avoid phototoxicity we generally recorded the SAN cells at a line rate of 1.53 or 3.78 ms to obtain 10,000 lines. Fluorescence was excited at 488 nm and emissions were collected at > 505 nm. A 63 × oil immersion objective was used to record [Ca2+]i in isolated SAN myocytes. We suppressed the background noise and analyzed the time-courses of Ca2+ fluorescence by pClamp software (ver.10.6.2.2, Molecular Devices). [Ca2+]i transients and LCRs were analyzed using ImageJ software 8. To identify the ramp phase of the [Ca2+]i transient, we integrated the F/F0 signal 150 ms before the peak of the cell-wide transient and calculated the slope of this phase using Clampfit (ver. 10.7).
Statistical analysis was performed using Prism 8.0 (GraphPad Software). Data are represented as mean ± the standard error of the mean (SEM) or the standard error (SD) as indicated. Statistical tests used in each experiment are specified throughout the figure legends. Statistical significance was defined as: *p < 0.05, **p < 0.05, p < 0.01, ***p < 0.001 and ****p < 0.0001.
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We thank Ira Cohen (University of New York, Stony Brook NY), Alicia D’Souza (University of Manchester, UK) and Jean-Yves Le Guennec (University of Montpellier) for critical reading of the manuscript. We thank Jörg Striessnig (University of Innsbruck, Austria) for support and Hee-Sup Shin (Institute for Basic Science, South Korea), for sharing the original Cav3.1−/− genotype. We also thank the staff of the iExplore BioCampus Facility of Montpellier for breeding the mouse lines.
The IGF group is a member of the Laboratory of Excellence Ion Channel Science and Therapeutics supported by a grant from ANR (ANR-11-LABX-0015). Research supported by the Fondation pour la Recherche Medicale “Physiopathologie Cardiovasculaire” (DPC20171138970 to M.E.M.), by the Agence Nationale de Recherche (ANR-15-CE14-0004-01 to M.E.M.) and the Fondation Leducq (TNE FANTASY 19CVD03 to Matteo E. Mangoni and Peter J. Mohler).
The authors declare no competing interests.
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Baudot, M., Torre, E., Bidaud, I. et al. Concomitant genetic ablation of L-type Cav1.3 (α1D) and T-type Cav3.1 (α1G) Ca2+ channels disrupts heart automaticity. Sci Rep 10, 18906 (2020). https://doi.org/10.1038/s41598-020-76049-7