American lobsters (Homarus americanus) imported live into Europe as a seafood commodity have occasionally been released or escaped into the wild, within the range of an allopatric congener, the European lobster (H. gammarus). In addition to disease and competition, introduced lobsters threaten native populations through hybridisation, but morphological discriminants used for species identification are unable to discern hybrids, so molecular methods are required. We tested an array of 79 single nucleotide polymorphisms (SNPs) for their utility to distinguish 1,308 H. gammarus from 38 H. americanus and 30 hybrid offspring from an American female captured in Sweden. These loci provide powerful species assignment in Homarus, enabling the robust identification of hybrid and American individuals among a survey of European stock. Moreover, a subset panel of the 12 most powerful SNPs is sufficient to separate the two pure species, even when tissues have been cooked, and can detect the introduced component of hybrids. We conclude that these SNP loci can unambiguously identify hybrid lobsters that may be undetectable via basic morphology, and offer a valuable tool to investigate the prevalence of cryptic hybridisation in the wild. Such investigations are required to properly evaluate the potential for introgression of alien genes into European lobster populations.
Genetic introgression due to hybridisation with non-native species is a major consequence of human-facilitated introductions that threatens endemic species with reduced fitness and local replacement1,2. Even where hybridisation between native and introduced species is rare, rapid and extensive genetic introgression can arise3. Population-level introgression and genetic admixture are not necessarily undesirable—managed interbreeding has been proposed to enhance resilience in key species threatened with extinction4—but invasive hybridisation is often associated with profoundly harmful effects, including loss of genetic variation and adaptation1,5. Nevertheless, assessing the conservation threat posed by hybridisation is not straightforward, and is especially challenging when hybrids themselves are fundamentally difficult to identify5,6. Investigation of hybridisation and management of its impacts require tools to distinguish hybrids from pure species strains, and the increasing availability of molecular markers presents a powerful resource to assess the extent of crossbreeding and introgression in wild populations subject to introductions7,8.
The European lobster (Homarus gammarus) is renowned for its high value as a seafood commodity, but stock collapses have severely diminished the productivity of fisheries throughout extensive portions of the species’ range9. In Europe, recent annual landings of H. gammarus of ~5,000 tonnes are dwarfed by those of its transatlantic congener, the American lobster (H. americanus), which supports vast harvests of >150,000 tonnes per year10. To satisfy European demand for lobster that native H. gammarus landings cannot fulfil, ~17,000 tonnes of whole American lobsters are imported annually, around three quarters of which are imported live11, with the remainder comprised mainly of whole animals which are shipped frozen having already been cooked12,13.
Perhaps inevitably, the import of live H. americanus has led to their introduction into European waters via their escape or release14,15. In one high-profile case from 2015, some 361 American lobsters were released during a religious ceremony in Brighton, UK, with some introduced females later recovered bearing fertilised eggs and hundreds more never recovered at all16. Lobsters captured in European habitats and reported as H. americanus by fishers, managers or scientists have led to concerns that its presence poses a threat to native species, especially H. gammarus14,17. Citing their potential for invasiveness and to threaten native lobsters via competition and genetic introgression, Norway banned the import of live H. americanus in 2016, while the same concerns led Swedish officials to launch a bid, ultimately unsuccessful, to add the species to the European Union’s list of invasive alien species11, which would have prohibited the live trade of American lobsters throughout the EU bloc.
Although concerns that pathogenic syndromes of H. americanus may spread to devastate H. gammarus stocks have been tempered by the latter’s apparent disease resilience18,19, fears that American lobster introductions may impact European populations through competition and genetic introgression have not been so readily mitigated, particularly following recent observations that Americans both predate on and interbreed with European counterparts20. Reports of wild hybridisation are concerning as well as surprising; while long established that Homarus hybrids could be bred in captivity through induced fertilisation21, it was also proposed that behavioural characteristics of sexual selection should constrain interspecific mating in the wild22. However, this appears not to be the case20. Molecular identification of suspected Homarus hybrids has to date been reliant on the microsatellite method of Jørstad et al. (23, cited in20), though neither the sequences nor assignment power of these three loci have ever been published. Reliance on so few loci can also limit power for the detection of hybridisation and introgression following backcrossing8.
Most lobsters reported as alien H. americanus in Europe are taxonomically identified on the basis of heterospecific morphological indicators, especially differences in exoskeleton pigmentation and the presence of a spine on the ventral surface of the rostrum that is absent in H. gammarus23,24. Hybrids, however, cannot be visually identified since they may display characteristics of either parent or a mixture of both, and these methods also have limitations in distinguishing the pure species; the ventral rostral spine is unreliable, with both species capable of displaying the supposedly diagnostic characteristic of the other, and exoskeletal colouration can vary broadly14,15,23. Moreover, colouration is effectively invalidated as a determinant by cooking, which turns the shell of both species red. This presents another means by which H. americanus may impact H. gammarus as a seafood commodity, albeit through economic, rather than ecological, competition. In the UK, a 400 g cooked native European lobster typically costs in excess of £2025, whereas an equivalent H. americanus imported from Canada retails at about half this price (e.g. £1126). Given this difference in value, the limitations of morphologically diagnostic characteristics present clear opportunity for exploitative mislabelling27, a widespread traceability issue impacting seafood supply chains.
The development of modern molecular resources is required to enable comprehensive assessments of the potential for H. americanus to threaten its congener in Europe through hybridisation in the wild, and to undermine market traceability via mislabelling. In this study, we tested single nucleotide polymorphism (SNP) markers, recently developed to assess H. gammarus population structure28,29, for their application in discerning the two Homarus species and their hybrids, including their utility with pre-cooked material, with the aim of developing universal and accurate molecular tools for species and hybrid assignment.
Clawed lobster tissues were all stored in 95–100% ethanol at −20 °C between sampling and DNA extraction. Tissue samples obtained were as follows:
pleopods of 20 cooked H. americanus, described as originating from Maine, USA, sourced frozen from a UK-based seafood importer and sampled in 2019.
pleopods of 30 cooked H. gammarus, originating from Cornwall, UK, sourced frozen from UK-based seafood suppliers and sampled in 2019.
thirty whole zoeal stage I larvae, expected H. americanus x gammarus hybrids, hatched and sampled at Kristineberg Marine Research Station in Fiskebäckskil, western Sweden, in May and June 2017 from an ovigerous female H. americanus (92 mm carapace length) caught near Bornö Island in Gullmar fjord in October 2016. Siblings from this clutch had previously been reported as hybrids using three unpublished microsatellites (20, using the method in23).
pereiopods of 18 live egg-bearing female H. americanus, comprising three individuals from each of six locations encompassing most of the species’ range (New Brunswick, Newfoundland, Prince Edward Island and Nova Scotia in Canada, and Massachusetts and New Hampshire in the USA). Tissues were collected by collaborating researchers.
Extraction of genomic DNA from these tissues was conducted using the salting-out technique of Jenkins et al.28. DNA yields were assessed using a Nanodrop One spectrophotometer and standardised to concentrations of 50–100 ng/μl, before electrophoresis on 1% agarose gels to check fragment quality. As expected, DNA from cooked samples frequently evidenced degradation via electrophoresis. Genotyping and scoring of the 96 SNP loci of Jenkins et al.28 was carried out on the Fluidigm EP1 system, following the protocols outlined in Jenkins et al.29. Two separate genotyping runs were conducted on all samples from cooked lobsters and expected hybrids to test repeatability of results. To provide reference samples and test the potential occurrence of hybrids among wild European lobster populations, these new data were added to a data set comprised of 1,278 H. gammarus genotyped by Jenkins et al.29. Quality control and filtering of genotype data followed the protocols outlined by Jenkins et al.29, except that the permissible missing data threshold was relaxed from 20% to 40% to ensure retention of three cooked samples that failed the stricter criteria.
Genetic divergence was initially explored using discriminant analysis of principal components (DAPC), executed in the adegenet v2.1.1 package30 in R31. Cross-validation to select the optimal number of principal components to retain was conducted using the xvalDapc function in adegenet. Two analyses were used to test the assignment of species and the detection of hybrids. Firstly, adegenet was used to run snapclust32 which uses the Expectation–Maximization algorithm to calculate maximum-likelihood estimations of genetic clustering and admixture. The number of expected genetic clusters (k) was set to 2, with explicit modelling for the presence of hybrids between these two clusters (hybrids = TRUE) and all other parameters run at default. Secondly, the program StrAuto v1.033 was used for parallel processing of the Bayesian clustering algorithm STRUCTURE v2.3.434, implemented across 10,000 MCMC repetitions following a burn-in of 10,000, assuming an admixture model but without prior sampling information (locprior off) and all other parameters run at default. STRUCTURE run replicates were merged using CLUMPP v1.1.235, and the optimal number of genetic groups (K) was defined using ΔK36 and mean L(K)34 with the pophelper v220.127.116.11 package37 in R. In addition, to test the performance of a reduced marker panel that could facilitate cheaper genotyping, the DAPC and snapclust analyses were repeated with a subset of the 12 most informative SNPs, as selected by DAPC loading contributions and inspection of allele frequencies (Fig. 1A). For this subset of loci, snapclust was run as above, both with and without expectations of hybrids being present.
As per Jenkins et al.29, filtering of the full 96-SNP data set removed 17 SNPs, leaving a panel of 79 SNPs (Supplementary Table S1), across which the mean level of missing data for all new samples was 3.0%. Among sample types, the mean level of missing data was 3.0% for hybrid larvae, 3.8% for cooked lobster samples, and 1.0% for live-sampled H. americanus. Aside from disparities caused by missing data, only 25 allele scores (0.4%) were mismatched between genotyping runs, all but three of which were attributed to inconsistent scoring at one locus.
Of the 79 SNP loci known to be highly polymorphic in H. gammarus29, only 12 were polymorphic across all H. americanus samples (n = 38), with 45 polymorphic among the clutch of hybrid siblings (n = 30). All except one of the 12-SNP subset were monomorphic in H. americanus, and the single locus to show heterozygosity did so in only one individual (Fig. 1A). DAPC showed clear and extensive separation of the two Homarus species across all 79 SNPs, with hybrids clustering together in a discrete group between these clades (Fig. 2). Cooked individuals clustered with the correct species clade, while there was also segregation between H. gammarus of Atlantic and Mediterranean origins, as demonstrated by Jenkins et al.29.
Using all 79 SNPs, snapclust successfully detected all individuals of H. americanus and hybrid lineages (Fig. 2; Supplementary Fig. S1), with membership probabilities >0.999 (Supplementary Table S2). All cooked lobsters also assigned to the correct species at probabilities >0.999. Only one of the 1,308 assumed European lobsters—an individual from Kåvra in Sweden—was assigned majority hybrid membership by snapclust (to a component of 68%), and was also allocated a pronounced H. americanus component (~25%) using STRUCTURE (Fig. 3). Analysis of STRUCTURE outputs confirmed that K = 3 was the best-supported number of clades, although STRUCTURE placed greater emphasis on the intraspecific differentiation between H. gammarus of Atlantic and Mediterranean origins, so did not allocate a specific cluster to hybrids. Nevertheless, all hybrid individuals were still differentiated, with clade composition defined as approximately half to the H. americanus cluster, and half among the two H. gammarus clusters (Fig. 3).
Using only genotypes from the 12-SNP subset, snapclust assigned all H. gammarus and H. americanus (including cooked samples) to the correct species clade when run without assumptions of hybridisation (Fig. 1B; Supplementary Fig. S1). Only four H. gammarus individuals were assigned to probabilities <0.995, and only one of these was <0.95 (Supplementary Table S2). Notably, majority H. americanus composition was also assigned to all known hybrids via this analysis (Fig. 1B). When snapclust assumed the presence of hybridisation, known hybrids were detected and effective assignment of H. americanus was maintained, but 37 H. gammarus individuals (2.8% of the total) were incorrectly assigned with a majority component to the hybrid clade. These results were supported by DAPC, which showed little separation between some outlying H. gammarus individuals and the hybrid cluster using just 12 SNPs (Supplementary Fig. S2).
Using 79 SNPs originally developed to investigate intraspecific genetic differentiation among H. gammarus populations, we were instead able to highlight interspecific differences between H. gammarus, its congener H. americanus, and hybrids of the two species. Effective determination of both species and their hybrids were confirmed with the full 79-SNP panel using the snapclust software. Robust assignment was maintained using a subset of the 12 most powerful SNPs when assumptions of hybridisation were omitted; all individuals of both pure species were assigned correctly, and the introduced genetic component of hybrids was still detected, such that hybrids were assigned as H. americanus rather than H. gammarus. These biallelic loci can define species in Homarus because, while highly polymorphic in H. gammarus, the majority (85%) were monomorphic in the 38 H. americanus individuals we tested.
Our results show that these SNP loci present useful genetic tools for accurate taxonomic determination of Homarus lobsters. A subset of just 12 SNPs discriminates H. americanus from H. gammarus, facilitating the use of a genotyping chip such as the Fluidigm Flex Six Genotyping IFC, which can screen 72 individuals across 12 SNPs, but which can be loaded in six discrete sections of only 12 individuals at a time. This technology is therefore useful for processing small sample runs quickly and relatively cheaply (~£4 per individual), so is well suited to applications of routine screening or opportunistic checks of specimens caught and/or sold in Europe which are suspected to be introduced and/or mis-sold H. americanus. We have also demonstrated that this method can successfully genotype and determine species of pre-cooked lobsters, which few consumers or industry personnel could identify visually, even when sold whole. In practical terms, this tool could be used to screen for the genetic signature of H. americanus among putatively European lobsters captured or marketed in Europe. Although this 12-SNP tool lacks the resolution to reliably identify hybrids without generating false positives among pure H. gammarus specimens, it could still be useful in identifying samples warranting further analysis with the full SNP panel to test for hybridisation, since all the hybrids we tested assigned as H. americanus when analytical expectations were for the two pure species only. If necessary, Homarus hybrids could then be distinguished from specimens of the two pure species using all 79 SNPs.
All 30 H. americanus x gammarus offspring from Sweden were assigned hybrid status by snapclust using our 79-SNP tool. Only one assumed H. gammarus (out of 1,306) assigned as a majority hybrid by snpaclust. This individual (‘Kav26’) was a 103 mm CL male sampled in 2007 from the Kåvra lobster reserve in western Sweden38, approximately 20 km from the capture location of the introduced H. americanus female which carried the hybrid larval clutch tested in this study, and in an area which has experienced frequent introductions of American lobsters in recent years20. As such, although it seems plausible that this individual’s assignment as a hybrid represents an anomalous false positive, we cannot rule out the possibility that this specimen had some H. americanus ancestry via interspecific breeding; that this individual originated from a designated conservation reserve may warrant further investigation. Our study includes only one hybrid sibling cohort, so it would be beneficial to confirm the power of this 79-SNP tool on other suspected hybrid clutches, although the extent of interspecific differentiation we revealed suggests it should be universally applicable for identifying first-generation Homarus crosses.
Currently, it is difficult to ascertain the threat posed to European lobster stocks from hybridisation with H. americanus, since conflicting information exists as to both the likelihood of interspecific mating and the fertility of resultant hybrids. Talbot et al.21 found that the spermatophores of captive-reared hybrid males lacked sperm, suggesting that they were infertile, but Kitaka (pers comm., as cited by22) achieved interspecific mating in captivity and asserted that both sexes of the resultant hybrid offspring were fertile. Fertile hybrids enable backcrossing and pervasive population-level introgression, although hybridisation is still a conservation threat even where hybrids are infertile, since it represents wasted reproductive effort5,6. Mate-choice of H. gammarus females is to favour conspecific males regardless of their size and dominance status22, so the discovery of female H. americanus bearing hybrid clutches in the wild in Europe suggests that either American females more readily mate interspecifically than their European counterparts, or that behavioural barriers are not sufficient to prevent interspecific mating where no conspecific mate can be found. Accordingly, we recommend investigation of the propensity for interspecific mating, between both sexes of both species, and of the fertility status of hybrids of both sexes.
The recent motion from some European scientists and regulators to take a conservative and proactive approach to the threats posed by introduced H. americanus by outlawing its live-trade was met with robust resistance from advocates of commerce initially valued at US$260 million a year to North American exporters12,13. Indeed, instead of restricting live-trade, a newly-adopted EU trade agreement eliminates 6–20% tariffs on Canadian exporters, providing them with duty-free access to European markets39. Although existing regulations prohibit the release of non-native lobsters across most of Europe, this has not been sufficient to prevent increasing numbers of both inadvertent and deliberate introductions of H. americanus, heightening the likelihood of it becoming permanently established and/or damage from hybridisation14,15,16. In the wake of our confirmation that interspecific crossbreeding between native and introduced lobsters has occurred in the wild, greater effort should be made to educate regulators, commercial traders and the general public as to the potential conservation threats posed by hybridisation between endemic and introduced lobsters.
Although clear opportunity exists to profit from the mislabelling of cheaper H. americanus as the more expensive H. gammarus, the extent to which erroneous species designation—whether inadvertent or fraudulent—currently impacts lobster supply chains is unknown, as is the extent to which hybridisation with introduced H. americanus may be impacting wild European lobster populations. Such investigations can now be implemented using these SNP resources. The cost of genetic techniques is often cited to justify why they are not more widely used to enforce regulatory standards in the seafood sector; in practice, however, the expense of molecular testing is typically far exceeded by financial penalties levied against uncovered incidences of malpractice40. Overall, the approaches we have tested offer more powerful assignment of species and hybridisation in clawed lobsters than existing methods based on allozymes41, random amplified polymorphic DNA42 or microsatellites23, and enable accurate and rapid sample processing via high-throughput SNP genotyping technology. These assignment techniques can be applied in forensic assessments to address the extent that introduced H. americanus threaten populations of H. gammarus via hybridisation and invasive introgression, and whether fraudulent mislabelling affects clawed lobster seafood products in Europe.
Muhlfeld, C. C. et al. Hybridization rapidly reduces fitness of a native trout in the wild. Biol. Lett. 5, 328–331 (2009).
Muhlfeld, C. C. et al. Legacy introductions and climatic variation explain spatiotemporal patterns of invasive hybridization in a native trout. Global Change Biol. 23, 4663–4674 (2017).
Goodman, S. J., Barton, N. H., Swanson, G., Abernethy, K. & Pemberton, J. M. Introgression through rare hybridization: a genetic study of a hybrid zone between red and sika deer (genus Cervus) in Argyll, Scotland. Genetics 152, 355–371 (1999).
Chan, W. Y., Peplow, L. M., Menéndez, P., Hoffmann, A. A. & van Oppen, M. J. Interspecific hybridization may provide novel opportunities for coral reef restoration. Front. Mar. Sci. 5, 160 (2018).
Rhymer, J. L. & Simberloff, D. Extinction by hybridization and introgression. Annu. Rev. Ecol. Syst. 27, 83–109 (1996).
Allendorf, F. W., Leary, R. F., Spruell, P. & Wenburg, J. K. The problems with hybrids: setting conservation guidelines. Trends Ecol. Evol. 16, 613–622 (2001).
Barilani, M. et al. Detecting hybridization in wild (Coturnix c. coturnix) and domesticated (Coturnix c. japonica) quail populations. Biol. Conserv. 126, 445–455 (2005).
Senn, H. V. et al. Distinguishing the victim from the threat: SNP-based methods reveal the extent of introgressive hybridization between wildcats and domestic cats in Scotland and inform future in situ and ex situ management options for species restoration. Evol. Apps. 12, 399–414 (2019).
Kleiven, A. R., Olsen, E. M. & Vølstad, J. H. Total catch of a Red-Listed marine species is an order of magnitude higher than official data. PLoS ONE 7, e31216, https://doi.org/10.1371/journal.pone.0031216 (2012).
FAO. Fisheries and Aquaculture Department, FIGIS Global Capture Statistical Query. Food and Agriculture Organisation of the United Nations, http://fao.org/fishery/statistics/global-capture-production/ query/en (2019).
Brockmark, S. Risk assessment of American lobster (Homarus americanus). Swedish Agency for Marine and Water Management, Report 2016:4 (2016).
Statistics Canada. Canadian Domestic Exports of Lobster, 2011–2016. CATSNET Analytics, http://lobstercouncilcanada.ca/wp-content/uploads/2017/03/Lobster-Exports.pdf (2017).
NOAA. Lobster, 2015-2016: Cumulative US Trade Data by Product. National Marine Fisheries Service, https://www.st.nmfs.noaa.gov/commercial-fisheries/foreign-trade/applications/trade-by-product (2019).
Jørstad, K.E., Agnalt, A.-L. & Farestveit, E. The introduced American lobster, Homarus americanus in Scandinavian waters, in In the Wrong Place-Alien Marine Crustaceans: Distribution, Biology and Impacts (eds. Galil, B., Clark, P. & Carlton, J.), 625-638. (Springer, 2011).
Stebbing, P. et al. Reports of American lobsters, Homarus americanus (H. Milne Edwards, 1837), in British waters. BioInvasions Records 1, 17–23 (2012).
Guardian Media Group. Two Buddhists fined £15,000 for releasing crustaceans into sea. The Guardian, Sept 23, 2017, https://theguardian.com/uk-news/2017/sep/23/two-buddhists-fined-15000-releasing-non-native-crustaceans-sea-brighton (2017)
Stebbing, P. Risk Assessment Summary Sheet: American Lobster. Great Britain Non-Native Species Secretariat, http://nonnativespecies.org/downloadDocument.cfm?id=1379 (2016a).
Davies, C. E. et al. A comparison of the structure of American (Homarus americanus) and European (Homarus gammarus) lobster cuticle with particular reference to shell disease susceptibility. J. Invertebr. Path. 117, 33–41 (2014).
Whitten, M. M. et al. Cuticles of European and American lobsters harbour diverse bacterial species and differ in disease susceptibility. Microbiol. Open 3, 395–409 (2014).
Øresland, V., Ulmestrand, M., Agnalt, A.-L. & Oxby, G. Recorded captures of American lobster (Homarus americanus) in Swedish waters and an observation of predation on the European lobster (Homarus gammarus). Can. J. Fish. Aquat. Sci. 74, 1503–1506 (2017).
Talbot, P., Hedgecock, D., Borgeson, W., Wilson, P. & Thaler, C. Examination of spermatophore production by laboratory-maintained lobsters (Homarus). J. World Mariculture Soc. 14, 269–278 (1983).
van der Meeren, G. I., Chandrapavan, A. & Breithaupt, T. Sexual and aggressive interactions in a mixed species group of lobsters Homarus gammarus and H. americanus. Aquat. Biol. 2, 191–200 (2008).
Jørstad, K. E. et al. Comparison of genetic and morphological methods to detect the presence of American lobsters, Homarus americanus (H. Milne Edwards, 1837; Astacidea: Nephropidae) in Norwegian waters. Hydrobiologia 590, 103–114 (2007).
Stebbing, P. Species Description and Identification Summary Sheet: American Lobster. Great Britain Non-Native Species Secretariat, http://nonnativespecies.org/downloadDocument.cfm?id=1177 (2016b).
Fish Society, UK. Shellfish products: ‘Cooked native lobster’, https://www.thefishsociety.co.uk (2019).
Waitrose, UK. Frozen Fish & Seafood: ‘Whole cooked Canadian lobster’, https://www.waitrose.com (2019).
Jacquet, J. L. & Pauly, D. Trade secrets: renaming and mislabelling of seafood. Mar. Policy 32, 309–318 (2008).
Jenkins, T. L., Ellis, C. D. & Stevens, J. R. SNP discovery in European lobster (Homarus gammarus) using RAD sequencing. Conserv. Genet. Res. 11, 253–257 (2018).
Jenkins, T. L., Ellis, C. D., Triantafyllidis, A. & Stevens, J. R. Single nucleotide polymorphisms reveal a genetic cline across the northeast Atlantic and enable powerful population assignment in the European lobster. Evol. Apps. 12, 1881–1889 (2019).
Jombart, T. & Ahmed, I. adegenet 1.3-1: New tools for the analysis of genome-wide SNP data. Bioinformatics 27, 3070–3071 (2011).
R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria, http://R-project.org/ (2019).
Beugin, M. P., Gayet, T., Pontier, D., Devillard, S. & Jombart, T. A fast likelihood solution to the genetic clustering problem. Methods Ecol. Evol. 9, 1006–1016 (2018).
Chhatre, V. E. & Emerson, K. J. StrAuto: automation and parallelization of STRUCTURE analysis. BMC Bioinformatics 18, 192 (2017).
Pritchard, J. K., Stephens, M. & Donnelly, P. Inference of population structure using multilocus genotype data. Genetics 155, 945–959 (2000).
Jakobsson, M. & Rosenberg, N. A. CLUMPP: A cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure. Bioinformatics 23, 1801–1806 (2007).
Evanno, G., Regnaut, S. & Goudet, J. Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study. Mol. Ecol. 14, 2611–2620 (2005).
Francis, R. M. pophelper: An R package and web app to analyse and visualize population structure. Mol. Ecol. Res. 17, 27–32 (2017).
Huserbråten, M. B. O. et al. Conservation, Spillover and Gene Flow within a Network of Northern European Marine Protected Areas. PLoS ONE 8, e73388, https://doi.org/10.1371/journal.pone.0073388 (2013).
Lobster Council of Canada. Canada-European Union Comprehensive Economic and Trade Agreement (CETA), http://lobstercouncilcanada.ca/market-access/canada-european-union-comprehensive-economic-and-trade-agreement-ceta (2019).
Martinsohn, J. T. et al. DNA-analysis to monitor fisheries and aquaculture: Too costly? Fish Fish. 20, 391–401 (2019).
van der Meeren, G. I., Ekeli, K. O., Jørstad, K. E. & Tveite, S. Americans on the wrong side – the lobster Homarus americanus in Norwegian waters. ICES CM 2000 / U 20, 1–15 (2000).
Hughes, G. & Beaumont, A. R. A potential method for discriminating between tissue from the European lobster (Homarus gammarus) and the American lobster (H. americanus). Crustaceana 77, 371–376 (2004).
We are indebted for the provision of lobster tissue samples to: Laura Benestan, Yann Dorant and Louis Bernatchez of Université Laval, for live H. americanus; Matt Sankey of Sankey’s Fishmongers Ltd., Tunbridge Wells, and Prawn on the Lawn Ltd., Highbury, London, for cooked H. gammarus; S & J Fisheries Ltd., Ivybridge for cooked H. americanus. We are also grateful to Ann-Lisbeth Agnalt of the Institute of Marine Research, Bergen, for helpful comments during the preparation of the manuscript, and to the National Lobster Hatchery, Padstow, and Wing of St Mawes Ltd., Indian Queens, for their collaboration throughout this research. The work underpinning this paper was supported through the Agri-tech Cornwall & the Isles of Scilly Research, Development and Innovation programme. Agri-tech Cornwall is funded by the European Regional Development Fund (ERDF), Cornwall Council and the Council for the Isles of Scilly (award number: 05R16P00366).
The authors declare no competing interests.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Ellis, C.D., Jenkins, T.L., Svanberg, L. et al. Crossing the pond: genetic assignment detects lobster hybridisation. Sci Rep 10, 7781 (2020). https://doi.org/10.1038/s41598-020-64692-z