Abstract
Plant autotrophic respiration is responsible for the atmospheric release of about half of all photosynthetically fixed carbon and responds to climate change in a manner different from photosynthesis. The plant mass-specific respiration rate (rA), a key parameter of the carbon cycle, has not been sufficiently constrained by observations at ecosystem or broader scales. In this study, a meta-analysis revealed a global relationship with vegetation biomass that explains 67–77% of the variance of rA across plant ages and biomes. rA decreased with increasing vegetation biomass such that annual rA was two orders of magnitude larger in fens and deserts than in mature forests. This relationship can be closely approximated by a power-law equation with a universal exponent and yields an estimated global autotrophic respiration rate of 64 ± 12 Pg C yr−1. This finding, which is phenomenologically and theoretically consistent with metabolic scaling and plant demography, provides a way to constrain the carbon-cycle components of Earth system models.
Similar content being viewed by others
Introduction
The amount of carbon dioxide (CO2) assimilated by photosynthesis is nearly equaled by the amount released back into the atmosphere by ecosystem respiration (defined as the sum of autotrophic plant respiration and heterotrophic microbial and animal respiration). Approximately half of the assimilated CO2 is released by autotrophic (mostly dark) respiration (RA), which varies with biotic and abiotic factors1,2. Autotrophic respiration, which involves complicated metabolic pathways, is a key determinant of carbon production, use efficiency, turnover, and ecosystem net carbon balance3,4. Although few observational and modeling studies have quantified RA at the global scale, many studies have estimated gross and net primary production (GPP and NPP, typically in Mg C ha−1 yr−1 at the ecosystem scale) and soil respiration (ground surface efflux of CO2 from plant roots and microbes)5. Studies of plant carbon-use efficiency (i.e., = NPP/GPP, or 1 − RA/GPP; empirically 0.22–0.79) have indicated that RA is not a constant fraction of GPP3,6. Most terrestrial carbon cycle and vegetation models estimate RA in a more simplified form than photosynthesis with respect to both biochemistry and empirical parameterization7. Specifically, most of these models parameterize RA on the basis of the growth–maintenance respiration paradigm8,9 (Supplementary Fig. S1). This scheme is phenomenological and practical, and it allows analysis of how respiration is regulated by cost-based components. However, its key coefficients (mass-specific plant organ respiration rates and environmental responsiveness) are poorly constrained by observations and are arbitrarily calibrated, leading to large carbon cycle uncertainties in Earth system models10,11. At present, many land models include a vegetation dynamic in which plant age and mass classes and competitive dynamics between then are explicitly simulated. Therefore, there is a clear need to devise a practical constraint for the behavior of these large-scale models.
Ecological theories and hypotheses
Here, it is hypothesized that the total plant autotrophic respiration rate (i.e.,including growth and maintenance, from roots to leaves) is size-dependent and should be based on biological constraints. Intuitively, large individuals, which tend to have lower specific surface areas, more inactive organs (woody stems and coarse roots), lower nitrogen concentrations, and slower growth rates (Supplementary Fig. S1), may be expected to have lower respiration rates. A more theoretical and quantitative interpretation is proposed here.
First, the accumulation of organ- and individual-scale measurements of plant gas fluxes and mass balances has led to a metabolic scaling theory of plant respiration12,13,14. These studies have demonstrated a power-law relationship between individual weight (WI, kg C per individual) and respiration rate (rI, g C per individual yr−1) across a wide range of plant sizes:
where a is a coefficient and α is the scaling exponent. Despite the increasing availability of global vegetation data (e.g., forest density15) thanks to satellite observations and dataset compilation, predicting large-scale (e.g. regional) RA from this relationship alone is highly challenging.
Meanwhile, at the population level, competition and self-thinning account for a negative relationship between plant density (N, individuals ha−1) and mean individual weight (WI) according to the following power law16:
where b is a coefficient and the exponent β reflects plant demographic effects. This power-law relationship was first established in studies of ideal monocultures, and the mechanisms underlying the density effect such as resource competition have been investigated16,17. Moreover, the relationship has been critically evaluated through studies on real-world vegetation and has now become a recognized ecological principle17. When combined with Eq. (1), the relationship between vegetation biomass (WV = N · WI, kg C ha−1 or Mg C ha−1) and mass-specific respiration rate (rA = RA / WV, g C kg C−1 yr−1) can be expressed by the following power-law:
where c is a coefficient. See Supplementary Information for a detailed derivation of Eq. (3).
Results and Discussion
Meta-analysis of scaling relationship
To determine the scaling relationship at larger spatial scales, I conducted a meta-analysis of vegetation biomass and RA at the ecosystem scale (typically 102–106 m2; see Methods for details). I used observational datasets from the literature from a variety of ecosystems ranging from infertile fens and deserts to mature tropical forests (148 records from 73 studies; Supplementary Table S1; Supplementary Fig. S2). The studies used a range of observational methods, including chamber measurements and biometric mass balances, which are each subject to certain errors and biases. Mass-specific respiration rates, rA, ranged from 14.6 g C kg−1 C yr−1 in a mature temperate conifer forest to 2588 g C kg−1 C yr−1 in a boreal peatland. Overall, rA was closely correlated with vegetation biomass by a power-law equation (Fig. 1) at both in situ and standardized temperatures:
where c′ is a coefficient. γ corresponds to the exponent in Eq. (3) and was estimated as −0.535 (95% confidence interval, −0.465 to −0.605) at in situ temperatures and −0.630 (−0.565 to −0.694) at 15 °C. This relationship covers a wide range of vegetation types (e.g., forests and non-forests), ages, and densities. The data deviate from the log–log relationship by up to a few orders of magnitude, and the coefficient of determination (R2) is lower than that for individual-scale studies on metabolic scaling (about 0.913,14). Nevertheless, Eq. (4) explains a remarkable 67–77% of the variance in vegetation rA, a much higher percentage than explained by latitude and temperature (Fig. 2). As discussed later, data obtained from multiple nearby sites with different disturbance histories (i.e., at at different points in a chronosequence) follow this scaling relationship. Also, the scaling exponent differed substantially from −1 (the exponent of the null model, where RA is independent of WV; see gray lines in Fig. 1), which implies that the relationship is biologically meaningful and does not merely reflect autocorrelation.
The derived relationship is consistent with biological constraints. Previously proposed values of α are 2/3 (surface-area to mass scaling), 3/4 (Kleiber’s law, mass–vascular branching), and 1.0 (isometric scaling)12,13. Typical values of the demographic coefficient β have been posited to be −1.605 (Reineke’s rule), −3/2 (Yoda’s rule), and −4/3 (fractal scaling)17. The value of the exponent in Eq. (4) obtained in the meta-analysis is consistent with values obtained using these α and β values; for example, γ = −0.535 (the value for in situ temperatures) can be obtained if α = 0.822 and β = −1.5; γ = −0.63 (the value at 15 °C) can be obtained if α = 0.75 and β = −1.66 (Supplementary Fig. S3). The consistency of the relationship is an encouraging sign, and warrants consideration of the mechanisms underlying the relationship and its usefulness for further study.
Comparison with vegetation models
The negative relationship between organism size and the mass-specific metabolic rate is well known18 and is represented phenomenologically in vegetation and carbon cycle models10,11. These models use different rA values for construction (growth-rate-dependent) and maintenance (standing-mass-dependent) respiration rates of leaves, stems, and roots. Thus, the allometric vegetation growth relationships assumed in models (e.g., the accumulation of woody tissues in mature forests) can be expected to simulate the size dependence of apparent rA. Several models also consider shifts in nitrogen concentrations, which are expected to correlate with synthetic activity, and the associated respiration rates of plant organs. I examined the mass–rA relationship in contemporary models using simulations from the Multi-scale Terrestrial Model Intercomparison Project (MsTMIP)19 (Supplementary Figs. S4 and S5).
Analyses of global data with 14 models revealed a negative power-law relationship between grid-cell biomass and rA (derived from RA and WV), with exponents ranging from −0.662 to −0.167 at 15 °C (Fig. 3). These values are mostly less negative than the value of exponent γ obtained from the meta-analysis, implying a weaker mass-dependence of rA in the models. Moreover, correlations between biomass and rA were also weaker (R2 was 0.055–0.534 in the models and 0.765 in the meta-analysis). The mass–rA relationship differed greatly among individual models, leading to different estimates of plant respiration rate at grid to global scales. Therefore, there is a clear need for an empirical relationship that could be used to constraint model parameterizations.
Estimation of global R A
To quantify the predictability of the mass–rA relationship (Fig. 1a; rA = 1194 · WV−0.535), I calculated RA globally using a 1-km mesh map of plant biomass (WV). Annual global RA was estimated to be 64.0 ± 12.0 Pg C yr−1 from 500 Pg C of plant biomass (Fig. 4), considering the uncertainty range of parameters. Assuming a typical value of global terrestrial GPP (120 Pg C yr−1)20, global NPP and carbon-use efficiency were estimated as 56 Pg C yr−1 and 0.467, respectively; these values are quite consistent with previous studies6. Although the RA map is derived solely from biomass data, the distribution of estimated RA, which ranges from <1 Mg C ha−1 yr−1 in dry and cold climates to >20 Mg C ha−1 yr−1 in tropical forests, appears reasonable. Because of the size dependence of rA, forests account for only 58.1% of global RA despite contributing 75.3% of global plant biomass. The mean and range of estimated RA is comparable to estimates from previous studies. For example, in MsTMIP model results, RA averaged 73.5 ± 20.0 (range, 48.2 to 120.8) Pg C yr−1 in 1991–2010. The inclusion of an independent estimate of heterotrophic respiration (51 Pg C yr−1) based on soil chamber observation data21 gives a total ecosystem respiration rate (the sum of autotrophic and heterotrophic respiration) of 115 Pg C yr−1, which is comparable to previous observation-based estimates and global CO2 syntheses (103–120 Pg C yr−1)20,22. The spatial distribution of RA is similar to that of photosynthetic productivity, which is closely correlated with RA (Supplementary Fig. S6), as estimated by up-scaling of flux measurements and remote sensing data. The difference in estimated RA between the present study and the MsTMIP models implies that the models may overestimate RA in the tropics (Fig. 4c), where high-biomass tropical rain forests predominate. Inadequate constraints on vegetation rA, perhaps caused by a failure to account for thermal acclimation in the models, may account for the difference.
Biological implications
Variations in respiration or metabolic rate are likely to have profound biological implications that go beyond what us captured by simple gain–loss carbon accounting1,23. In the relationship presented in this study, rA is determined by biomass and is independent of productivity. In reality, however, the lower rA of developed ecosystems with high biomass is not only a consequence of senescence but also an adaptive strategy in resource-limited environments. In this study, I tested the power-law relationship and the behavior of its exponent in terms of plant metabolic scaling and demography, but my interpretation does not fully account for other factors such as temperature and nitrogen availability. Thermal acclimation of plant respiration may to some extent account for the large variations in plant respiration seen in the meta-analysis (e.g., the lower rA of tropical forests than on boreal forests)24,25. See Supplementary Fig. S7 for a meta-analysis using a response function that includes thermal acclimation26. Notably, the regression line estimated using this response function has a larger exponent (i.e., a steeper biomass dependence of rA) but a lower coefficient of determination. In this regard, several chronosequence studies conducted at multiple nearby sites with different disturbance histories (e.g., elapsed time since the last fire) are useful to specify the scaling relationship, irrespective of temperature conditions. These studies have indicated that mass-specific respiration clearly decreases with ecosystem development (i.e., increasing total and mean individual biomass) (Supplementary Fig. S8). Nutrient limitation (e.g., nitrogen stoichiometry) may also affect this relationship through co-limitation and isometric scaling with plant nutrient content27. For example, plants subject to more severe nutrient limitation have a smaller biomass stock and need to invest more metabolic energy (associated with respiratory CO2) to extract and assimilate nutrients from the soil.
Despite the fact that RA accounts for a large fraction of ecosystem CO2 emissions, it has not been adequately quantified to date. Total ecosystem respiration and soil respiration have been measured5,28, but their separation into emission components remains difficult. Most measurements of RA in large plants, except for those using individual-tree chambers14 rely on indirect or destructive methods. Inadequate data quality and quantity have thus made examining RA difficult. The recent compilation of various plant trait measurements into databases has facilitated global analyses of functional properties. Relevant data on plant leaf, stem, and root rA in the public TRY database29 showed the range to be comparable to the estimate obtained in my meta-analysis (Fig. 3). The rA values in the TRY database clearly differ among plant organs (from 4.5 ± 6.9 kg C kg−1 C yr−1 for stems to 11.1 ± 11.5 kg C kg−1 C yr−1 for leaves). Although this could enable a trait-based way to analyze plant properties globally10, mass-based information on plant respiration remains limited. The data cover only a fraction of plant diversity (1453 species for leaf respiration), and there are many fewer mass-based measurements than surface area-based measurements (e.g., for stem respiration, n = 26756 surface area-based measurements and 920 mass-based measurements). In addition to the expansion of trait-based databases, developments in remote sensing have provided more data that is relevant to terrestrial carbon budgets such as aboveground biomass30. Direct measurements of RA from remote sensing platforms, however, remain out of reach.
Conclusion
The present study provides an effective basis for reducing uncertainties in RA values simulated in carbon cycle and Earth system models. As reported by previous global carbon cycle syntheses22,31, current evaluations of the global carbon cycle are still subject to considerable uncertainty. The present study may help constrain terrestrial ecosystem models, which have among the largest uncertainties. In practice, model parameters should be constrained or optimized so that the simulated rA and RA come close to the likely range expected from the empirical relationships. The non-linear relationship between vegetation biomass and rA also highlights the necessity of high-resolution method to obtain accurate estimates of RA in heterogeneous areas. The prospect of applying empirical constraints to dynamic vegetation models, which are being implemented in Earth system models, is especially promising. The RA model described in this paper is certainly applicable to transitional states of vegetation associated with disturbance and climate change, and other mechanisms could be added to account for RA responses to pollutants and extreme weather (e.g., droughts and heat waves) to fully explain variations in rA. Moreover, plant leaves have a second respiratory mechanism, photorespiration, which is regulated by different factors from the dark respiration that is the focus here. Integrating biological factors such as nitrogen dependence and thermal acclimation, in conjunction with empirical constraints as presented here, may further improve the parameterization of respiration.
Methods
Data collection for meta-analysis
Data used in the meta-analysis were obtained from two main sources – Internet searches on (1) Web of Science (Clarivate Analytics, Philadelphia, PA, USA) and (2) Google Scholar (Alphabet, Mountain View, CA, USA) – using keywords such as “autotrophic respiration”, “ecosystem”, “forest”, and “carbon cycle”. I collected original papers as much as possible and looked for data on ecosystem-scale autotrophic respiration, heterotrophic respiration, phytomass (plant biomass carbon stock), and soil organic carbon stock. I used observed annual values for respiration rates; to avoid extrapolation biases, daily to seasonal values were excluded. I also collected supplementary records from open-access datasets provided as part of several syntheses on the terrestrial carbon cycle32,33,34. Here again, I consulted original papers as much as possible to reduce data-extraction errors.
I then developed a database comprising records from the literature (Supplementary Table S1). Several sites reported multiple values derived by using different assumptions and correction methods; these were included in the analyses to assess the range of uncertainty caused by data handling. For each record, I collected site information for ancillary analyses: site latitude, land-cover type, annual mean temperature, annual precipitation, plant individual density, stand age (mostly for forests), basal area, canopy height, leaf area index, and so on. For ecosystem-scale carbon stock and respiration, units were standardized to Mg C ha−1 and Mg C ha−1 yr−1, respectively. Dry weight was converted to carbon weight by multiplying by a coefficient of 0.45; conversion from CO2 weight to carbon weight was done by multiplying by 12/44. For several studies, total autotrophic respiration rate was obtained by summing component fluxes from plant organs; data that lacked major components (e.g., only aboveground respiration) were therefore not used.
Most respiration measurements in these studies were conducted by the chamber method. Specific respiration rates of vegetation components (e.g., leaf, stem, and root) were measured with cuvettes and then scaled up to ecosystem scale. Few direct measurements of whole-ecosystem autotrophic and heterotrophic respiration have been conducted at ecosystem scale because of practical constraints. Note that ecosystem-scale fluxes measured by the eddy-covariance method quantify net ecosystem CO2 exchange only; photosynthetic assimilation and ecosystem respiration were then estimated from net fluxes by using appropriate separation methods such as non-linear regression.
Statistical analyses were conducted in SPSS Statistics v. 25 (IBM Inc., Armonk, NY, USA). To obtain 95% confidence intervals for the regression coefficients (e.g., scaling exponents in the form of power laws), bootstrapping was conducted 1000 times. The null model was based on the null hypothesis that vegetation respiration rate is independent of biomass (Supplementary Fig. S9).
Temperature correction of plant respirationrates
In general, the temperature response function, f(T), is described as:
where Ea is the activation energy (0.6 eV for metabolic rate), and k is Boltzmann’s constant (8.62 × 10−5 eV k−1). The temperature response of plant (and microbial) respiration is often parameterized as an exponential function with a parameter Q10 (increase per 10 °C temperature rise) as:
where T0 is the base temperature (for example, 15 °C) at which f(T) takes the value 1. In many models, this function is applied to maintenance respiration, whereas the construction respiration is assumed to be proportional to growth rate. Thus, as a result of changes in maintenance and growth components, the apparent f(T) can change through time and between places. When standardizing the respiration rates obtained under different temperature conditions, the data were divided by f(T) values to convert them into the value at the base temperature, for example:
In Fig. 1b, a Q10 value of 2.0 was used for this conversion. Moreover, as a result of thermal acclimation, Q10 varies seasonally and geographically. The relationship between temperature and foliar respiration Q10 has been summarized as follows26:
Terrestrial model simulation outputs
Global simulation outputs of RA and WV were derived from the MsTMIP35 dataset, available from https://daac.ornl.gov/cgi-bin/dsviewer.pl?ds_id=1225. This study uses outputs of 14 models, which provide data on autotrophic respiration and plant biomass at a spatial resolution of 0.5° × 0.5° in latitude and longitude. For carbon cycle models (GTEC, LPJ-wsl, ORCHIDEE, SiBCASA, VEGAS2.1, and VISIT), the results of the MsTMIP SG3 experiment were used. The models were driven by time-series data on atmospheric CO2, climate, and land-use change. For carbon–nitrogen models (BIOME-BGC, CLASS-CTEM-N, CLM4, CLM4VIC, DLEM, ISAM, TEM6, and TRIPLEX-GHG), the results of the MsTMIP BG1 experiment were used. The models were driven by time-series data on atmospheric CO2, nitrogen deposition, climate, and land-use change. For each model and cell, values of RA and WV were averaged for the period 1991–2010. When standardizing temperature at 15 °C, grid temperature was obtained from CRU TS3.236.
Plant trait TRY database
Values of rA for different plant organs were downloaded from the TRY database29 (https://www.try-db.org/TryWeb/Home.php; accessed 30 July 2019). This study used the following open access datasets: “Leaf respiration rate in the dark per leaf dry mass (trait no. 41)” (n = 10,719), “Root respiration rate per root dry mass (trait no. 514)” (n = 1161), and “Stem respiration rate per stem dry mass (trait no. 519)” (n = 540). These data were obtained by many different researchers for various plant species under different observational conditions. For each organ, the average, standard deviation, median, and 25% and 75% quartiles were calculated.
Global R A estimation
The global value of RA and its estimation range were obtained by applying the meta-analysis regression equation to the global map of vegetation biomass37 (Fig. 4a). The calculation was conducted at approximately 1 km (30″ in latitude and longitude) resolution. Global total RA was estimated as 64.0 Pg C yr−1 by using the equation in Fig. 1a; a sensitivity analysis showed that it varies from 60.4 to 66.4 Pg C yr−1 with a ± 10% change in biomass at each grid. The range of estimation uncertainty was obtained by perturbing coefficients in the regression equation: from the meta-analysis, standard deviations were 159.0 for the multiplier coefficient and 0.0358 for the exponent. Also, vegetation biomass was perturbed by ±10% standard deviation in each cell. Equation coefficients and biomass were randomly sampled 1000 times, and used independently for estimation of global RA. Finally, the average and standard deviation were calculated.
Data availability
The meta-analysis dataset used in this study is available from the Figshare repository (https://doi.org/10.6084/m9.figshare.10252694.v1) and is attached as Supplementary Table S1.
References
Amthor, J. S. Respiration and Crop Productivity. (Springer-Verlag, 1989).
Ryan, M. G. Effects of climate change on plant respiration. Ecol. Appl. 1, 157–167 (1991).
Piao, S. et al. Forest annual carbon cost: a global-scale analysis of autotrophic respiration. Ecology 91, 652–661 (2010).
Heskel, M. A. Small flux, global impact: Integrating the nuances of leaf mitochondrial respiration in estimates of ecosystem carbon exchange. Am. J. Bot. 105, 815–818, https://doi.org/10.1002/ajb2.1079 (2018).
Bond-Lamberty, B. & Thomson, A. Temperature-associated increases in the global soil respiration record. Nature 464, 579–582, https://doi.org/10.1038/nature08930 (2010).
Collalti, A. & Prentice, I. C. Is NPP proportional to GPP? Waring’s hypothesis 20 years on. Tree Physiol. 39, 1473–1483, https://doi.org/10.1093/treephys/tpz034 (2019).
Gifford, R. M. Plant respiration in productivity models: conceptualisation, representation and issues for global terrestrial carbon-cycle research. Func. Plant Biol. 30, 171–186 (2003).
McCree, K. J. Equations for the rate of dark respiration of white clover and grain sorghum, as functions of dry weight, photosynthetic rate, and temperature. Crop Sci. 14, 509–514 (1974).
Thornley, J. H. M. & Cannell, M. G. R. Modelling the components of plant respiration: representation and realism. Ann. Bot. 85, 55–67 (2000).
Atkin, O. K. et al. Global variability in leaf respiration in relation to climate, plant functional types and leaf traits. New Phytol. 206, 614–636, https://doi.org/10.1111/nph.13253 (2015).
Huntingford, C. et al. Implications of improved representations of plant respiration in a changing climate. Nature Comm. 8, https://doi.org/10.1038/s41467-41017-01774-z (2017).
Enquist, B. J. et al. Scaling metabolism from organisms to ecosystems. Nature 423, 639–642 (2003).
Reich, P. B., Tjoelker, M. G., Machado, J.-L. & Oleksyn, J. Universal scaling of respiratory metabolism, size and nitrogen in plants. Nature 439, 457–461 (2006).
Mori, S. et al. Mixed-power scaling of whole-plant respiration from seedlings to giant trees. Proc. Nat. Acad. Sci. USA 107, 1447–1451, https://doi.org/10.1073/pnas.0902554107 (2010).
Crowther, T. W. et al. Mapping tree density at a global scale. Nature 525, 201–205, https://doi.org/10.1038/nature14967 (2015).
Yoda, K., Kira, T., Ogawa, H. & Hozumi, K. Self-thinning in overcrowded pure stands under cultivated and natural conditions (intraspecific competition among higher plants XI). J. Biol. Osaka City Univ. 14, 107–129 (1963).
Weller, D. E. A reevaluation of the −3/2 power rule of plant self-thinning. Ecol. Monogr. 57, 23–43 (1987).
Makarieva, A. M. et al. Mean mass-specific metabolic rates are strikingly similar across life’s major domains: Evidence for life’s metabolic optimum. Proc. Nat. Acad. Sci. USA 105, 16994–16999, https://doi.org/10.1073/pnas.0802148105 (2008).
Huntzinger, D. N. et al. Uncertainty in the response of terrestrial carbon sink to environmental drivers undermines carbon-climate feedback predictions. Sci. Rep. 7, https://doi.org/10.1038/s41598-017-03818-2 (2017).
Le Quéré, C. et al. Global carbon budget 2018. Earth Sys. Sci. Data 10, 2141–2194, https://doi.org/10.5194/essd-10-2141-2018 (2018).
Hashimoto, S. et al. Global spatiotemporal distribution of soil respiration modeled using a global database. Biogeosci. 12, 4121–4132, https://doi.org/10.5194/bg-12-4121-2015 (2015).
Yuan, W. et al. Redefinition and global estimation of basal ecosystem respiration rate. Global Biogeochem. Cycles 25, https://doi.org/10.1029/2011GB004150 (2011).
O’Leary, B. M. et al. Variation in leaf respiration rates at night correlates with carbohydrate and amino acid supply. Plant Physiol. 174, 2261–2273, https://doi.org/10.1104/pp.17.00610 (2017).
Heskel, M. A. et al. Convergence in the temperature response of leaf respiration across biomes and plant functional types. Proc. Nat. Acad. Sci. USA 113, 3832–3837, https://doi.org/10.1073/pnas.1520282113 (2016).
Tjoelker, M. G. The role of thermal acclimation of plant respiration under climate warming: Putting the brakes on a runaway train? Plant Cell Environ. 41, 501–503, https://doi.org/10.1111/pce.13126 (2018).
Atkin, O. K. & Tjoelker, M. G. Thermal acclimation and the dynamic response of plant respiration to temperature. Tr. Ecol. Evol. 8, 343–351, https://doi.org/10.1016/S1360-1385(03)00136-5 (2003).
Reich, P. B. et al. Scaling of respiration to nitrogen in leaves, stems and roots of higher land plants. Ecol. Lett. 11, 793–801, https://doi.org/10.1111/j.1461-0248.2008.01185.x (2008).
Falge, E. et al. Seasonality of ecosystem respiration and gross primary production as derived from FLUXNET measurements. Agr. For. Meteorol. 113, 53–74 (2002).
Kattge, J. et al. TRY plant trait database – enhanced coverage and open access. Global Change Biol. 26, 119–188, https://doi.org/10.1111/gcb.14904 (2020).
Ciais, P. et al. Current systematic carbon-cycle observations and the need for implementing a policy-relevant carbon observing system. Biogeosci. 11, 3547–3602, https://doi.org/10.5194/bg-11-3547-2014 (2014).
Ciais, P. et al. Carbon and other biogeochemcial cycles. In: Climate Change 2013: The Physical Science Basis. The Fifth Assessment Report of the Intergovernmental Panel on Climate Change. (Cambridge University Press, 2013).
McGuire, A. D. et al. Interactions between carbon and nitrogen dynamics in estimating net primary productivity for potential vegetation in North America. Global Biogeochem. Cycles 6, 101–124 (1992).
Luyssaert, S. et al. CO2 balance of boreal, temperate, and tropical forests derived from a global database. Global Change Biol. 13, 2509–2537, https://doi.org/10.1111/j.1365-2486.2007.01439.x (2007).
Bond-Lamberty, B. & Thomson, A. A global database of soil respiration data. Biogeosci. 7, 1915–1926, https://doi.org/10.5194/bg-7-1915-2010 (2010).
Huntzinger, D. N. et al. The North American Carbon Program Multi-scale Synthesis and Terrestrial Model Intercomparison Project: Part 1: Overview and experimental design. Geosci. Model Dev. 6, 2121–2133, https://doi.org/10.5194/gmd-6-2121-2013 (2013).
Harris, I., Jones, P. D., Osborn, T. J. & Lister, D. H. Updated high-resolution grids of monthly climatic observations – the CRU TS3.10 Dataset. Int. J. Climatol. 34, 623–642, https://doi.org/10.1002/joc.3711 (2014).
Aaron, R. & Gibbs, H. K. (ed. Oak Ridge National Laboratory Carbon Dioxide Information Analysis Center, Oak Ridge, Tennessee, USA.) (2008).
Acknowledgements
This work was supported by a Japan Society for Promotion of Science KAKENHI grant (No. 17H01867). MsTMIP data were obtained from the Oak Ridge National Laboratory data server. TRY data were obtained from the TRY project home page.
Author information
Authors and Affiliations
Contributions
AI designed the study, conducted analyses, and drafted this manuscript.
Corresponding author
Ethics declarations
Competing interests
The author declares no competing interests.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Ito, A. Constraining size-dependence of vegetation respiration rates. Sci Rep 10, 4304 (2020). https://doi.org/10.1038/s41598-020-61239-0
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-020-61239-0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.