Type2 diabetes-associated nephropathy is the commonest cause of renal failure. Mechanisms responsible are controversial. Leptin-deficient hyperphagic Zucker (fa/fa) rats were modeled to test the hypothesis that glomerular enlargement drives podocyte hypertrophic stress leading to accelerated podocyte detachment, podocyte depletion, albuminuria and progression. By 6weeks, prior to development of either hyperglycemia or albuminuria, fa/fa rats were hyperinsulinemic with high urinary IGF1/2 excretion, gaining weight rapidly, and had 1.6-fold greater glomerular volume than controls (P < 0.01). At this time the podocyte number per glomerulus was not yet reduced although podocytes were already hypertrophically stressed as shown by high podocyte phosphor-ribosomal S6 (a marker of mTORC1 activation), high urinary pellet podocin:nephrin mRNA ratio and accelerated podocyte detachment (high urinary pellet podocin:aquaporin2 mRNA ratio). Subsequently, fa/fa rats became both hyperglycemic and albuminuric. 24 hr urine albumin excretion correlated highly with decreasing podocyte density (R2 = 0.86), as a consequence of both increasing glomerular volume (R2 = 0.70) and decreasing podocyte number (R2 = 0.63). Glomerular podocyte loss rate was quantitatively related to podocyte detachment rate measured by urine pellet mRNAs. Glomerulosclerosis occurred when podocyte density reached <50/106um3. Reducing food intake by 40% to slow growth reduced podocyte hypertrophic stress and “froze” all elements of the progression process in place, but had small effect on hyperglycemia. Glomerular enlargement caused by high growth factor milieu starting in pre-diabetic kidneys appears to be a primary driver of albuminuria in fa/fa rats and thereby an under-recognized target for progression prevention. Progression risk could be identified prior to onset of hyperglycemia or albuminuria, and monitored non-invasively by urinary pellet podocyte mRNA markers.
Obesity and type2 diabetes mellitus prevalence continues to increase worldwide reflecting population access to high energy food and reduced calorie expenditure necessary to sustain life1,2,3. Diabetes-associated end stage kidney disease (ESKD) is the commonest cause of renal mortality with attendant human and monetary costs1,2,3,4.
Micro-albuminuria is recognized as an early marker for both obesity and diabetes-associated nephropathy (DAN), with progressive disease being associated with increasing albuminuria, progressive glomerulosclerosis, and accumulating nephron loss eventually culminating in ESKD5. Diverse mechanisms contribute to nephron loss including injuries to glomerular, tubule-interstitial and vascular compartments6,7.
Normal podocyte density (number per glomerular tuft volume) is necessary to maintain high resolution glomerular filtration through complete coverage of the filtration surface area by foot processes8. At the same time podocytes have limited capacity for replacement such that critical reduction of podocyte density (caused by increased glomerular volume and/or reduced podocyte number) is associated with proteinuria, glomerulosclerosis and ESKD in all progressive glomerular diseases so far examined8,9,10,11,12,13,14,15,16.
In type1 and type2 diabetes in man reduced podocyte density is associated with both glomerular enlargement and reduced podocyte number17,18,19. Podocyte injury is present at an early stage and podocyte depletion occurs with advanced DAN17,18,19. Animal model studies implicate a role for mTORC1, the major integrator of growth signaling, in driving podocyte and glomerular injury that reproduces many features associated with progressive DAN in man20,21.
We recently reported that increased glomerular volume and impaired podocyte capacity to adapt to hypertrophic stress are both necessary to cause proteinuria and progressive glomerulosclerosis in a non-diabetic rat model12,15. Furthermore, reducing the rate of glomerular growth by calorie intake reduction, even when started late and by modest amounts, prevented downstream consequences manifest by podocyte depletion, proteinuria and glomerulosclerosis. Since glomerular enlargement is well-documented in diabetes, and reducing food intake is a therapy available worldwide, we tested the hypothesis that glomerular enlargement could also drive albuminuria and progression in the Zucker rat model of type2 diabetes. In this model homozygous (fa/fa) leptin deficiency causes loss of inhibitory feedback for satiety thereby resulting in primary hyperphagia that drives the cardinal features of type2 diabetes including body weight gain, hyperinsulinemia, peripheral insulin resistance, hyperglycemia and glycosuria. The model also develops progressive albuminuria and glomerulosclerosis, and thereby parallels many aspects of both obesity and type2 DAN in man22,23,24.
Homozygous fa/fa rats were already significantly heavier than Fa/fa controls by 6weeks of age and continued to gain weight faster than control rats prior to onset of overt hyperglycemia (Fig. 1A). In association with persistent high level glycosuria and by 18weeks fa/fa rat weight had plateaued and by 30weeks body weight had decreased to below that of Fa/fa control which continued weight gain through 46weeks of observation. By 10weeks fa/fa rats had developed overt hyperglycemia (Fig. 1B) associated with glycosuria (Fig. 1C), and increased urine volume due to glycosuria-associated osmotic diuresis (Fig. 1D). 24 hr urine albumin excretion in fa/fa rats became significantly increased by 10weeks, continued to increase to high levels by 38weeks and plateaued thereafter (Fig. 1E,F). Significant albuminuria did not develop in Fa/fa control rats. Blood pressure measured by tail cuff remained in the normal range (systolic 110–130 mmHg) with no significant difference between fa/fa and Fa/fa controls at any time point (data not shown).
Histologic changes and morphometry
Fig. 1G,H, Supplemental Fig. 1A,B and Fig. 2 shows representative histological findings and morphometry at 6, 15, 30 and 46weeks in fa/fa and Fa/fa controls. Glomeruli of fa/fa rats appeared larger than those of Fa/fa rats at each time point. As shown in Fig. 2A, by 6weeks (prior to development of overt diabetes or albuminuria) glomerular volume of fa/fa rats was already 1.6-fold greater than glomerular volume of Fa/fa rats (P < 0.01). The podocyte number per glomerulus in fa/fa rat glomeruli was slightly but significantly higher than in control littermates at 6weeks (P = 0.04) (Fig. 2B). Podocyte number per glomerulus decreased throughout the time course in fa/fa rats with a slope of −1.49/week (P < 0.01), but did not decrease significantly in Fa/fa controls. Podocyte density (number per volume) in fa/fa rats was decreased compared to Fa/fa controls by 6weeks (P < 0.01) and continued to decrease so that by 46weeks the podocyte density had reached low levels of 50 × 106/um3 associated with development of glomerulosclerosis (Fig. 2C). Glomerular volume per podocyte in fa/fa rats was increased compared to Fa/fa controls by 6weeks (P < 0.01) and continued to increase over the 46week observation period (Fig. 2D). Total podocyte cell volume per glomerulus and average individual podocyte cell volume were already 1.5-fold and 1.3-fold respectively above control at 6weeks (P < 0.01) and became increasingly different from control over the 46week observation period (Fig. 2E,F). In fa/fa rats by 30weeks PAS-positive expanded mesangial matrix was present (Fig. 1G) as also indicated by the increasing non-podocyte glomerular volume (Fig. 2G). By 46weeks expanded PAS-positive mesangial matrix was present in glomeruli (Fig. 1G) in association with segmental sclerosis lesions in 47 ± 28% of glomeruli of fa/fa as assessed by Glepp1 peroxidase and AZAN staining (Fig. 1H and Supplemental Fig. 1A).
Relationship of morphometric parameters to albuminuria
24 hr urine albumin excretion was plotted against glomerular morphometric parameters (Fig. 3). There was a very strong relationship between podocyte nuclear density and 24 hr albuminuria (R2 = 0.86, P < 0.01) (Fig. 3A). Since podocyte density is the ratio of glomerular volume to podocyte number per glomerulus these parameters were separately plotted (Fig. 3B,C). The relationship of 24 hr urine albumin to glomerular volume showed an R2 = 0.70 (P < 0.01). Similarly, there was a strong relationship between 24 hr urine albumin and podocyte number per glomerulus (R2 = 0.63, P < 0.01). Thus decreased podocyte density caused initially by increased glomerular volume alone, and subsequently by a combination of decreasing podocyte number compounded by increasing glomerular volume is strongly associated with albuminuria. However, fa/fa rat podocytes also appeared to be more susceptible than Fa/fa podocytes to hypertrophic stress as shown by the higher level of albuminuria at a density of 100 podocytes/106um3 (Fig. 3A), implying that a podocyte susceptibility factor was also required for albuminuria to be present.
High growth milieu and podocyte hypertrophic stress
Fig. 4A–C show that at 6weeks, prior to onset of glycosuria or albuminuria, blood insulin levels were 6.4-fold higher in fa/fa rats than in Fa/fa controls, and IGF1 and IGF2 (measured as 24 hr urine excretion) were 3.2-fold and 2.1-fold increased respectively. These data reflect an increased growth milieu in fa/fa kidneys. At this time, glomerular volume and average podocyte volume in fa/fa rats were already 1.6-fold and 1.3-fold above control respectively (Fig. 2A,F). Furthermore, the ratio of glomerular volume to total podocyte volume was higher in fa/fa than in Fa/fa controls (P < 0.01) (Fig. 2D) demonstrating that podocyte hypertrophy had not kept pace with glomerular volume increase. Growth factors and nutrients drive cell growth via the mTORC1 pathway in part by phosphorylation of ribosomal protein S6. As shown in Fig. 4D,E, by 6weeks podocyte ribosomal S6 phosphorylation was already significantly increased in fa/fa rat versus controls, compatible with hypertrophic podocyte stress being already present by 6weeks.
Urinary mRNA markers of podocyte hypertrophic stress
Hypertrophic podocyte stress causes accelerated podocyte detachment which can be monitored non-invasively by measuring podocyte mRNAs in the urine pellet12,15. Figure 5A–D shows 24 hr urinary pellet mRNAs for two podocyte markers (NPHS2/podocin and NPHS1/nephrin) and a distal tubular/collecting duct marker (aquaporin2). Also shown is the podocin:aquaporin2 mRNA ratio as a measure of the relative rate of podocin mRNA excretion in relation to a tubular mRNA marker (aquaporin2). Figure 5C confirms that 24 hr urinary pellet aquaporin2 mRNA levels were comparable between fa/fa and Fa/fa rats over the time-course in spite of large differences in urine volume between fa/fa and Fa/fa rats. By 6weeks, prior to onset of hyperglycemia or albuminuria, both the urinary pellet podocin mRNA and podocin:aquaporin2 mRNA ratio in fa/fa rat were already significantly increased above that of controls (P = 0.04), compatible with hypertrophic podocyte stress causing accelerated podocyte detachment at this early time point. Urinary podocin mRNA and podocin:aquaporin2 mRNA ratio subsequently increased reaching a plateau by 22weeks and persisted throughout the 46weeks of study at an average 88-fold higher levels in fa/fa rat urine compared to Fa/fa rat controls over the period of observation. The relative level of two podocyte-specific mRNAs (podocin and nephrin) is a non-invasive measure of podocyte stress caused by relative down-regulation of nephrin versus podocin10,25. Figure 5E shows that by 8weeks, prior to development of hyperglycemia or albuminuria, the urinary podocin:nephrin mRNA ratio was already significantly increased above control (P < 0.01). Figure 5F–J show that the changes observed in the urinary pellet were also present in renal cortex at the 30week time point.
The high nutrient and growth factor milieu in fa/fa rats might be expected to facilitate accelerated cell cycling as a part of the growth process. To assess this possibility, we used Ki67 as a cell cycle marker and WT1 and PAX8 as podocyte and parietal epithelial cell (PEC) markers respectively. As shown in Supplemental Table 1 although Ki67 labelling was present in glomeruli, interstitial and tubular compartments in both fa/fa and Fa/fa rats, there was no statistically significant difference between these groups. Furthermore, we were not able to identify double-label WT1/Ki67 positive cells (or double label PAX8/Ki67 PECs) in fa/fa rat kidney although double labelled cells were detectable in 2day old kidneys (Supplemental Fig. 2), suggesting that if de novo podocyte or PEC replenishment was occurring it was below the threshold of detection.
Food intake reduction (CR) “freezes” the progression process in place
If excess nutrient intake causes the altered kidney milieu that in turn causes podocyte hypertrophic stress and accelerated detachment leading to proteinuria and glomerulosclerolsis, then reducing nutrient intake should halt this process. To test this hypothesis rats were allowed to develop diabetes up to 15weeks on an ad-libitum diet and were then placed on a 40% reduced food intake (CR) diet in comparison to an ad-libitum fed group. As shown in Fig. 6 and Supplemental Fig. 3, CR had the effect of stabilizing (“freezing”) all parameters at the level they were at when CR was initiated at 15weeks of age. Thus, although CR rats had statistically significantly less weight gain and lower blood glucose levels, hyperglycemia persisted at high levels over the 15weeks of observation (from week15 to week30). Ad libitum fed fa/fa rat 24 hr urinary albumin excretion continued to increase to 100 mg/day, while CR rats maintained stable albuminuria at about 20 mg/day. Histology showed increased PAS-positive mesangial matrix in ad-libitum fed but not CR rats (Fig. 6F,G). Morphometry (Fig. 7A–E) showed that CR rats had lower glomerular volume (P = 0.05), lower glomerular volume per podocyte and non-podocyte glomerular volume (P < 0.01) and a higher podocyte nuclear density (P < 0.05), although podocyte number per glomerulus was not significantly different (P = 0.30). Thus CR rats at 30weeks had similar morphometric parameters to the ad-libitum fed rats at 15weeks (see Fig. 2), consistent with CR slowing or halting growth-induced processes.
Figure 7C shows that the effect of CR was to stabilize podocyte density at a level of 82 podocytes/106um3 which, from Fig. 3A, would be predicted to correspond to a 24 hr urinary albumin excretion of about 20 mg/day similar to that observed in Fig. 6E. In contrast, the ad-libitum fed rat podocyte nuclear density decreased to 65/106um3 primarily as a result of continued glomerular growth. From Fig. 3A a podocyte density of 65/106um3 is predicted to correspond to a 24 hr urinary albumin excretion rate of close to 100 mg/day as observed in Fig. 6E. Thus the observed 24 hr urinary albumin excretion values correspond closely to the values predicted by the podocyte density measurements thereby providing additional support for the concept that podocyte density is the major determinant of albuminuria.
Effect of CR on the growth milieu and podocyte hypertrophic stress
Fig. 7F–I shows that CR was associated with lower urinary IGF1/2 excretion. Furthermore, after 15weeks of CR glomerular ribosomal phosphor-S6 positive podocytes were reduced from 78% to 28% (P < 0.01) demonstrating that CR also reduced mTORC1-induced hypertrophic podocyte stress.
Reduced podocyte stress on the CR diet could be monitored by reduced urine podocyte mRNA markers
Figure 8 shows that CR was associated with a reduced rate of podocyte detachment as measured in both urinary pellet and in renal cortex (P < 0.01). Although the cortical podocin:nephrin mRNA ratio was reduced by CR (P < 0.05), it was not reduced in the urinary pellet (Supplemental Fig. 3), suggesting that detaching podocytes were those that were stressed and had a high urinary podocin:nephrin mRNA ratio.
Insulin and insulin-like growth factor (IGF-1/2) signaling (IIS pathway) in mammals, characteristically activated by nutrients and inhibited by starvation, is the paralogue of the insulin-like receptor daf-2 in the nematode C. elegans that has provided remarkable insights into relationships between nutrition, growth and longevity26,27,28. IGF-1 under the influence of both growth hormone and local factors is a major driver of kidney growth29. Cell growth is coordinated through mTORC1 activation driven both by growth factors binding to cell surface receptors and nutrient sensing of amino acids and glucose30,31. Hyperphagic fa/fa rats with secondary hyperinsulinemia, high IGF1/2, and large nutrient intake are therefore predisposed to activate mTORC1 and thereby to grow rapidly, including rapid glomerular growth. At the same time the podocyte is a structurally complex cell with limited capacity to divide or hypertrophy rapidly. Furthermore, primary dysregulation of the mTORC1 pathway in podocytes is proven to cause thickened GBM, proteinuria and other diabetes-like pathologic changes in the absence of hyperglycemia12,15,20,21,32. Thus, in fa/fa rats a high growth milieu can drive both glomerular volume enlargement and podocyte mTORC1 activation. In this setting the stressor (glomerular enlargement) and the susceptibility factor (podocyte mTORC1 activation) combine to cause accelerated podocyte detachment, albuminuria, glomerulosclerosis, and ultimately progression to ESKD, as previously reported for a non-diabetic model12,15.
Kriz and Lemley have previously emphasized that podocyte loss from glomeruli occurs primarily by detachment33. We therefore evaluated whether podocyte mRNA markers detected in the urinary pellet could quantitatively account for the number of podocytes lost from fa/fa rat glomeruli over the 40week period of observation. As shown in Supplemental Table 2, we estimate that >70% of podocytes lost from glomeruli could in fact be accounted for in the urinary pellet. Non-invasive markers that quantitatively reflect podocyte stress prior to hyperglycemia or albuminuria onset and that non-invasively report the rate of podocyte detachment from glomeruli have potentially clinical utility. We have previously reported similar associations in other models10,11,12,15 as well as in human Alport syndrome14,34, IgA nephropathy35, anti-GBM disease16 and allograft failure36. Other investigators have reported similar findings37,38,39,40,41,42.
By 8–10weeks in homozygous fa/fa rats overt diabetes reflected by hyperglycemia, glycosuria and polyuria was present. Increased albumin excretion was also present by 10weeks, and reached high levels by 38weeks. At first sight albuminuria might seem attributable to hyperglycemia itself, however, decreasing podocyte density is an alternative explanation for the progressive increase in albuminuria. This is an important distinction because if proteinuria and progressive glomerulosclerosis are significantly caused by glomerular volume enlargement this would identify an under-recognized therapeutic target for preventing progression. If glomerular enlargement causing podocyte hypertrophic stress is indeed a major driver of albuminuria in the fa/fa rat then one would expect that there would be a direct relationship between the 24 hr urinary albumin excretion and glomerular volume itself, as was observed (R2 = 0.70, P < 0.001). Furthermore, if the effect of glomerular volume enlargement in causing albuminuria was through podocyte hypertrophic stress then one would expect that podocyte density (glomerular volume in relation to podocyte number) would show an even stronger relationship with albuminuria than did glomerular volume alone, as was observed (R2 = 0.86, P < 0.001). Glomerulosclerosis was present in fa/fa rat glomeruli by 46weeks when podocyte density had reached low levels (50/106um3) as a result of the combination of glomerular volume enlargement and podocyte depletion. Podocyte density at this level is associated with glomerulosclerosis in all models and human glomerular diseases so far examined12,13,14,15,16,17,18,19. These data are therefore compatible with prior work showing that glomerular volume increase per se in the setting of podocyte susceptibility to hypertrophic stress drives albuminuria and glomerulosclerosis12,13,14,15,16.
Further support for this concept is provided by the food reduction (CR) study initiated at 15weeks after hyperglycemia and albuminuria were already established. In spite of persistent hyperglycemia, CR essentially “froze” the progression process in place such that all parameters including urinary IGF1/2, glomerular enlargement, podocyte density reduction, podocyte mTORC1 activation (ribosomal phosphor-S6), podocyte detachment rate, urinary podocyte markers and albuminuria remained at the stage they were at when CR was initiated. This suggests that podocytes were able to adapt to a lower stress level over the 15weeks of CR in spite of persistent hyperglycemia. Therefore, in the fa/fa rat hyperglycemia per se did not appear to be either necessary to initiate progression nor sufficient to drive the progression process once established.
Translation of fa/fa rat findings to man has important caveats. First, is the fa/fa rat a model of human obesity-associated nephropathy or type2 DAN or both? Obesity in the absence of overt diabetes is well-known to be associated with glomerular enlargement, proteinuria and development of FSGS lesions43. Furthermore, in this setting reducing food intake through bariatric surgery and other approaches ameliorates proteinuria44. On the other hand, type2 DAN also results from excessive food intake causing weight gain/obesity and hyperinsulinemia with superimposition of insulin resistance sufficient to cause pancreatic islet beta cell failure resulting in overt diabetes, as also occurs in the fa/fa rat model. Therefore, the fa/fa rat also resembles type2 DAN, particularly with respect to childhood type2 diabetes45. A second related question is that although the fa/fa rat replicates morphologic characteristics found in association with type2 DAN including basement membrane thickening, PAS-positive mesangial expansion and high level proteinuria22, like most but not all other rodent models, it does not exactly replicate major nodular glomerulosclerosis considered to be a characteristic pathologic phenotype of DAN23,24. However, in reality, there is a wide range of pathology observed in biopsies performed on proteinuric diabetics with the proportion of biopsies showing non-classical diabetic features ranging from 14–83%46 with 22% having FSGS47. The data in this report taken together with previous morphometric studies using a model of growth-induced glomerular failure12,15 and model of both type1 and type2 DAN in man17,18,19,48,49,50, would be consistent with an underlying process initiated by a combination of glomerular enlargement with podocyte susceptibilities. Podocyte susceptibilities could result from the growth factor milieu triggering podocyte mTORC1 activation, hyperglycemia level and exposure time (Fig. 9), metabolic factors including reactive oxygen species, mutations in podocyte-expressed genes such as the Col4α3 gene recently reported to be associated with MODY51, superimposed hypertension and other factors. According to this perspective the fa/fa rat models key elements common to both obesity and type2 diabetes-associated nephropathies. Importantly, these events can be monitored non-invasively using urine pellet podocyte mRNAs from an early stage prior to onset of hyperglycemia or albuminuria.
Materials and Methods
The study was approved by the University of Miyazaki Animal Research Committee (approval numbers: 2011-539 and 2017-501). Authors confirm that all the experiments are performed in accordance with approved guidelines.
Rat model of diabetic nephropathy
Male leptin-deficient Zucker (fa/fa) rats that develop type2 diabetes (n = 6) were compared with heterozygous Fa/fa rat littermates (n = 7) as non-diabetic controls. Rats were purchased from Charles River Breeding, Yokohama, Japan at 5weeks old. Open kidney wedge biopsy was performed with removal of about 1/10th of the kidney at 15 and 30 weeks. Kidneys were harvested at 46weeks at euthanasia when kidneys were perfusion-fixed with paraformaldehyde/lysine/periodate (PLP) and paraffin-embedded prior to sectioning.
Food intake reduction study (calorie reduction, CR)
5 weeks old male fa/fa rats (150 g) were fed ad-libitum until 15weeks of age when they were randomly assigned to either: (i) ad-libitum diet (n = 5), or (ii) 40%CR (n = 6). Food delivered to the 40%CR group was calculated by measuring the food eaten by the ad-libitum group at the same age. Rats were euthanized at 30weeks when kidneys were perfusion-fixed for analysis.
RNA analysis and qRT-PCR assay
Rats were housed in metabolic cages for overnight timed urine collection (average 17 hr) every 4weeks to measure urine volume, albuminuria, urinary glucose and urinary podocyte mRNA markers (podocin and nephrin) and aquaporin2 mRNA excretion. Urine was centrifuged at 4 °C for 15 min at 3200 × g on a tabletop centrifuge. The supernatant was removed, the pellet suspended in 1.5 mL diethyl pyrocarbonate-treated phosphate-buffered saline, and then centrifuged at 12,000 × g for 5 min at 4 °C. Diced rat cortex from kidney biopsy samples and washed urine pellets were suspended in RLT/β-mercaptoethanol buffer (RNeasy kit; Qiagen, Germantown, MD, USA) and then frozen at −80 °C11,16. RNA from the urinary pellet and kidney cortex were purified using an RNeasy mini kit (cat. No. 74106; Qiagen). cDNAs were reverse-transcribed from total RNA (~1 μg) using a high-capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA, USA). Quantitation of podocin, nephrin, aquaporin2 and glyceraldehyde-3-phospate dehydrogenase (GAPDH) mRNA abundance was performed with a LightCycler 96® system (Roche Molecular System, Mannheim, Germany) using FastStart Essential DNA Probe Master Mix (Roche Molecular System, Inc.) in a final volume of 10 μL per reaction. TaqMan probes (Applied Biosystems) used were: rat NPHS1 (nephrin) (cat. No. Rn00575235_m1); rat NPHS2 (podocin) (cat. No. Rn00709834_m1); rat aquaporin 2 (cat. No. Rn00563755_m1); rat GAPDH (cat. No. Rn01775763_m1). cDNA standard curves were constructed using these serially-diluted standards11,16. For analysis all urine measurements were adjusted to 24 hours.
Podometric analysis and phosphor-S6 immunostaining
Immunofluorescence and immunoperoxidase staining used PLP-perfused paraffin-embedded kidney sections. Mean glomerular radius (r) for 30–50 consecutive glomerular profiles was measured moving systematically from outer cortex to juxtamedullary glomeruli using the WinROOF imaging software (Mitani Corp., Tokyo, Japan). Mean maximal R = 4/πr and mean glomerular volume = 4/3πR3 were calculated16,52. Podocyte nuclear number was measured using Wilms’ Tumor 1 (WT1) immunofluorescence staining using 1.5um thick sections by ImagePro software (Media Cybernetics, Rockville, MD, USA) in 30–50 glomerular tufts52. Podocyte nuclear density was estimated using a quadratic equation developed to correct for section thickness and nuclear shape52. Average podocyte nuclear number per glomerular tuft = average glomerular volume x podocyte nuclear density. GLEPP1-positive tuft area was estimated from 30–50 consecutive glomerular tuft areas as the %glomerular tuft area staining positive by GLEPP1 immunoperoxidase using the WinROOF imaging software16,52. Average total podocyte volume was estimated by multiplying the mean glomerular volume by the mean %GLEPP1-positive area. The non-podocyte glomerular volume was estimated by subtracting total GLEPP1 podocyte volume from glomerular volume. Reagents: WT1 primary antibody (SC-7385 monoclonal IgG1; Santa Cruz Biotechnology, USA) 1:50 with Cy3-labeled secondary antibody (Jackson ImmunoResearch Laboratories, USA) 1:100. Phospho-S6 primary antibody (#2211 polyclonal; Cell Signaling Technology, USA) 1:400 with FITC-labeled secondary antibody (Jackson ImmunoResearch Laboratories, USA) 1:100. Slides were mounted using SlowFade Gold antifade reagent with DAPI (S36939; Invitrogen, USA). Anti-GLEPP1 mouse monoclonal antibody (1B4) at 1:100 (Wiggins laboratory) with immunoperoxidase Vectastain Elite ABC kit (PK-6100; Vector Laboratories Inc, USA).
WT1 or PAX8/Ki67 double label analysis
In each section cells were characterized as to whether they were podocytes (WT1 positive nuclei) or PECs (PAX8 positive nuclei) according to specific markers and whether they were non-podocyte glomerular tuft cells, periglomerular or interstitial cells or tubular cells according to their position in relation to other cortical structures. Within these compartments cells whose nuclei were Ki67 positive were counted for Fa/fa (6week), fa/fa (6week), Fa/fa (30week), fa/fa (30week), Fa/fa (45week) and fa/fa (45week) groups (n = 5–6). Averaged values for the 12 consecutive images were used for each rat. Thus in the case of WT1 and PAX8 cell nuclei would be double-labelled for WT1/Ki67 or PAX8/Ki67 respectively, or in the case of other compartments would be labelled by Ki67 alone. Double immunofluorescent labeling was performed on 1.5um thick sections using the cell cycle marker Ki67 (Cat # NB600-1252, Novus Biologicals, USA) and either the podocyte nuclear marker WT1 or the parietal cell (PEC) marker PAX8 (Cat # ab53490, Abcom, USA) with DAPI (blue) staining. Compound images from the red, green, and blue channels for 12 consecutive glomerular profiles were made for each rat at 200x magnification with a glomerulus in the center of the field.
Estimated by a blinded observer using AZAN-stained histologic sections as %glomerulosclerosis for 30–50 consecutive glomeruli.
Urinary IGF1, IGF2 and serum insulin
24 hr urinary IGF1 and IGF2 excretion were measured using Rat IGF1 and IGF2 ELISA kit (Cat No. MBS824704 and MBS824828, MyBioSource.com, USA). Serum insulin was evaluated using Mercodia Rat Insulin ELISA kit (Mercodia AB, Sweden).
GraphPad PRISM software, version 6.0 (GraphPad Software, Inc., USA) was used. Supplemental Table 1 data are expressed as mean ± SD, other data as mean ± SEM. Differences among two groups were tested using the Mann–Whitney U test, and more than two groups were tested using the Kruskal–Wallis test. When the result of the Kruskal–Wallis test was significant, a Dunn test was performed for post hoc analysis. Correlations between parameters were compared using Spearman’s rank correlation coefficient analysis. Correlation P values were calculated using Stata 15 I/C (College Station, TX). A P-value < 0.05 was considered statistically significant.
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We thank Su Q. Wang and Mahboob Chowdhury for technical assistance. We acknowledge the research helping by Ms. Ofuku, Kawano, Otsu, Kawagoe, Nagata and Mr. Yamasaki, and the secretarial assistance by Ms. Tahara. A.F. acknowledges the support of research grant from the JSPS KAKENHI (Grant Number JP16K19198), the Japan Foundation for Applied Enzymology (Grant Number 15V005), the Mitsubishi Tanabe Pharma Corporation (Grant Number MTPS20160507013) and Takeda Science Foundation. R.W. was supported by the National Institutes of Health (grants R01 DK46073 and 102643).
The authors declare no competing interests.
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Minakawa, A., Fukuda, A., Sato, Y. et al. Podocyte hypertrophic stress and detachment precedes hyperglycemia or albuminuria in a rat model of obesity and type2 diabetes-associated nephropathy. Sci Rep 9, 18485 (2019). https://doi.org/10.1038/s41598-019-54692-z
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