Mannose phosphotransferase system (Man-PTS) is the main mannose permease in bacteria but it is also a known receptor for subclass IIa bacteriocins (pediocin-like group) as well as subclass IId lactococcin A (LcnA) and lactococcin B (LcnB) (LcnA-like group). Subclass IIa bacteriocins exhibit a strong activity against Listeria spp. but they are not against Lactococcus spp. In contrast, the LcnA-like bacteriocins act only against Lactococcus lactis strains. Garvicin Q (GarQ) is a subclass IId bacteriocin with minor similarity to LcnA-like bacteriocins and a relatively broad antimicrobial spectrum including, among others, Listeria and Lactococcus spp. To identify the GarQ receptor, we obtained GarQ-resistant mutants of Lactococcus garvieae IBB3403 and L. lactis IL1403 and sequenced their genomes that revealed mutations in genes encoding the membrane-bound Man-PTS IIC or IID subunits encoded by ptnCD in L. lactis and manCD in L. garvieae. This is the first time that a bacteriocin outside the pediocin- and LcnA-like groups is shown to target Man-PTS. The interaction between GarQ and Man-PTS may occur through a new binding pattern involving specific amino acids highly conserved among the GarQ-sensitive bacterial species located in the N-terminal part and extracellular loops of subunit IID and in transmembrane region of IIC.
The emergence of antibiotic-resistant pathogens and an increasing consumer demand for more natural food preservatives have recently created an urgent need for new effective antimicrobials. One of the most promising groups of such compounds are bacteriocins, ribosomally synthesized antimicrobial peptides or proteins with a bactericidal or bacteriostatic activity. They are produced by both Gram-positive and Gram-negative bacteria, but those produced by Gram-positive bacteria, particularly lactic acid bacteria (LAB), show the greatest potential for application since bacteriocin-producing LAB strains can be used as starter and bioprotective cultures in the food industry1. The current classification of bacteriocins from Gram-positive bacteria divides them into two major classes: class I bacteriocins known as lantibiotics, which undergo post-translational modifications such as the generation of the thioether amino acids lanthionine and 3-methyllanthionine, and class II bacteriocins, so-called nonlantibiotics, which contain non-modified peptides or peptides with only minor modifications such as disulfide bridge or end-to-end circularization2, 3. The latter class has gained substantial interest due to their ubiquitous production by bacteria, great diversity in terms of amino acid sequence, physicochemical properties and target specificity, as well as width of inhibitory spectrum. The nonlantibiotics are further divided into (i) subclass IIa - pediocin-like bacteriocins, which are divided further into four (I-IV) groups and contain a conserved N-terminal amino acid sequence YGNGVXC, (ii) subclass IIb of two-peptide bacteriocins, (iii) subclass IIc of cyclic bacteriocins, and (iv) subclass IId of non-pediocin-like one-peptide bacteriocins4, 5.
The bacteriocins differ from each other by their inhibitory spectra, each targeting a defined set of bacteria, which suggests that they recognize specific receptors on the target cells6. However, the exact targets and molecular mechanisms underpinning this process are still almost unknown for most bacteriocins2. To date, only five bacteriocin receptors have been described for class II bacteriocins: (i) mannose phosphotransferase system (Man-PTS) recognized by all subclass IIa and a few subclass IId bacteriocins7; (ii) maltose ATP-Binding Cassette (ABC) transporter for garvicin ML, a circular subclass IIc bacteriocin8, (iii) the Zn-dependent metallopeptidase YvjB for the subclass IId LsbB9, (iv) undecaprenyl pyrophosphate phosphatase (UppP) for the subclass IIb lactococcin G10, and (v) a putative amino acid transporter for the subclass IIb plantaricin JK11.
Man-PTS belongs to the family of phosphoenolpyruvate dependent sugar transporters and can simultaneously import and phosphorylate selected monosaccharides, such as mannose, glucose, glucosamine or fructose. Man-PTS consists of the cytoplasm-located components IIA and IIB, which often occur as a single polypeptide chain, and transmembrane subunits IIC and IID, through which the sugars are transported12. Early studies showed that Man-PTS, apart from its principal sugar-transporter function, could also serve as a receptor for subclass IIa bacteriocins. For example, Listeria monocytogenes mutants resistant to pediocin-like bacteriocins (pediocin PA-1, leucocin A) displayed reduced expression of the Man-PTS-encoding mptACD operon13, 14. Moreover, inactivation of the mptACD operon in L. monocytogenes and Enterococcus faecalis caused resistance to mesentericin Y10515, 16. Conversely, the heterologous expression of the mptACD operon in insensitive Lactococcus lactis strains conferred sensitivity to leucocin A, pediocin PA-1 and enterocin A17. The studies above provide strong genetic evidence between the receptor genes and the associated sensitivity to the bacteriocins when the receptor genes are intact and expressed. However, a direct evidence of a contact between the receptor and the bacteriocin was still missing in these studies. This conclusive piece of evidence came from a study by Diep et al., 2007 who showed by immuno-precipitation, a direct interaction between the Man-PTS complex and a bacteriocin upon its binding to target cells, and further delimited the receptor functions to the membrane-located components IIC and IID of the complex7. Surprisingly, also two subclass IId bacteriocins, LcnA and LcnB, unrelated to the subclass IIa bacteriocins, have been reported to use Man-PTS subunits IIC and IID as a receptor7.
The Man-PTSs can be divided into several phylogenetic groups and so far only members of phylogenetic group I have been shown to serve as receptors for class II bacteriocins. The group I Man-PTSs contain three distinct regions termed α, β and γ localized in the IIC (α and β) and IID (γ) subunits6. Region α is indispensable for specific binding of subclass IIa bacteriocins and has been found to be present in the sensitive L. monocytogenes strains and absent in the resistant L. lactis strains. A distinct spectrum of activity has been observed for Man-PTS-targeting subclass IId bacteriocins LcnA and LcnB active only against L. lactis species18.
GarQ is a subclass IId bacteriocin produced by Lactococcus garvieae BCC 43578 isolated from fermented-pork sausage19. It uses a so called double-glycine leader for secretion and the mature peptide is 50 amino acids long. GarQ has a fairly broad antimicrobial spectrum being active against several species from the genera Bacillus, Enterococcus, Lactobacillus, Lactococcus and Pediococcus. Despite lacking the YGNGVXC motif characteristic for pediocin-like bacteriocins, GarQ is also active against L. monocytogenes 19.
In this study we report the identification of the receptor for garvicin Q, which turned out to be the IIC and IID subunits of Man-PTS. We show that despite recognizing the same receptor on the same host cells (L. lactis), GarQ and LcnA-group bacteriocins differ in their mode of interaction with Man-PTS. This difference may involve specific interactions between the bacteriocin and individual amino acids localized in the Man-PTS subunits IIC and IID. We experimentally identify the amino acids of Man-PTS from L. lactis and L. garvieae potentially responsible for GarQ binding and show that they are not involved in the binding of LcnA-group bacteriocins. The same seven amino acids are required for GarQ binding in both L. garvieae and L. lactis Man-PTS. Most of these amino acids are conserved in Man-PTSs from other GarQ-sensitive species, suggesting a similar mode of interaction with GarQ. These data indicate that across bacterial species Man-PTS may constitute an important target specifically recognized by multiple non-homologous bacteriocins.
Materials and Methods
Bacterial strains and culture conditions
The bacterial strains used in this study are listed in Table S1 in the Supplementary File. Indicator strains used to determine the activity spectrum of a bacteriocin, strains used to obtain spontaneous GarQ-resistant mutants, and L. lactis IL1403 with a deletion or complementation of the Man-PTS-encoding operon were grown in brain heart infusion (BHI) medium (Oxoid, Hampshire, United Kingdom) at 30 °C. Lactococcus spp. used to obtain spontaneous GarQ-resistant mutants with missense mutations were grown in chemically defined medium (CDM)20 supplemented with 1% mannose (man-CDM) at 30 °C. Escherichia coli EC1000 was grown in Luria-Bertani (LB) medium (Becton, Dickinson and Company, New Jersey, United States) at 37 °C. To grow E. coli EC1000 with pNZ8037 plasmid, chloramphenicol was added to 20 µg/ml and to grow L. lactis IL1403 with pNZ9530 and pNZ8037 plasmids, erythromycin and chloramphenicol to 5 µg/ml each were added to the growth medium. Transcription of the ptnCD genes cloned in pNZ8037 under the nisin-responsive promoter (Supplementary Table S1) was induced by the addition of nisin to between 0.1 and 10 ng/ml. Sensitivity to bacteriocins was determined by an inhibitory spectrum assay as described below. Soft agar (soft BHI-agar, soft man-CDM-agar) and agar plates (BHI-agar, man-CDM-agar) were prepared by adding agar (Merck, Darmstadt, Germany) to the final concentration of 0.75% and 1.5%, respectively, to appropriate liquid media.
Bacteriocin in the form of lyophyllisate with a purity of over 95% was obtained by a commercial chemical synthesis (PepMic, Suzhou, China). Before use, peptide was dissolved to the required concentration in 0.1% trifluoroacetic acid (TFA) (Sigma, Darmstadt, Germany).
Inhibitory spectrum assay
To assess the activity spectrum of a bacteriocin, indicator bacteria were inoculated and grown overnight (o/n) in BHI liquid medium, then 100 µl of the o/n culture was used to inoculate 5 ml of soft BHI-agar pre-heated to 55 °C. The molten agar was immediately poured onto a BHI-agar plate and distributed evenly. Following agar setting, 5 µl of suitable bacteriocin dilutions (0.03; 0.06; 0.125; 0.25; 0.5; 1 mg/ml) was spotted on selected locations on the plate. The plates were allowed to dry and were incubated o/n at 30 °C.
Selection of GarQ-resistant mutants
GarQ-resistant mutants of L. garvieae IBB3403 and L. lactis IL1403 were collected from small colonies that occasionally appeared in the inhibition zones during inhibitory spectrum assay or were selected in a dedicated experiment. For this purpose to 5 ml of soft BHI-agar or soft man-CDM-agar containing 100 µl of an indicator o/n-culture, 100 µl of suitable bacteriocin dilutions (as above) was added and the plates were incubated for single colonies to appear. Bacterial species identity was confirmed by 16 S rDNA sequencing using primers 27 F and 1492 R (Supplementary Table S1). To determine the level of resistance of the isolated GarQ-resistant mutants they were grown on microtiter plates with serial two-fold bacteriocin dilutions. The assays were performed in triplicate. The minimum inhibitory concentration value (MIC50) was determined as the lowest concentration of bacteriocin that resulted in reduction of bacterial growth by more than 50%. Stability of the resistance phenotype was confirmed by passaging the bacteria on plates without bacteriocin and then repeating the microtiter plate assay.
Genomic DNA isolation, DNA sequencing and data analysis
DNA was isolated using Genomic Mini kit (A&A Biotechnology, Gdynia, Poland). Samples for genome sequencing were prepared using Nextera XT DNA Sample Preparation kit, Nextera XT Indexing kit (96-indexes) and PhiX control V3 kit and sequenced on a Miseq Sequencer (Illumina, San Diego, Ca, United States). For each strain about 1.5–2.5 million 250-nt paired-end reads were obtained. The data were analyzed with CLC Genomics Workbench 6.5.2 (Qiagen, Hilden, Germany) and DNAStar SeqMan Gen (DNAStar, Madison, Wi, United States). Samples for manABCD operon (from L. garvieae) sequencing were prepared by PCR on genomic DNA with primers manCfor/rev and manDfor/rev (Supplementary Table S1). Samples for ptnABCD operon (from L. lactis) sequencing were prepared by PCR on genomic DNA with primers ptnCfor/rev and ptnDfor/rev (Supplementary Table S1). Amplified DNA was purified using Wizard® SV Gel and PCR Clean-Up System (Promega, Fitchburg, Wi, United States). To translate a nucleotide sequence to protein sequence, the translate tool on the ExPasy online server was used21. To compare protein sequences, the Clustal Omega software at the EMBL-EBI online server22 or MultAlin software23 were used. For prediction of transmembrane protein regions, the HMMTOP server was used24. Predicted topology of the membrane Man-PTSs subunits was visualized using Protter25. To predict a secondary GarQ structure, the fully automated protein structure homology-modelling SWISS-MODEL server was used26.
Phenotypic profiling and growth tests
Fermentation patterns of 49 sugars under anaerobic conditions were determined using the API 50 CH test (BioMerieux, Marcy l’Etolie, France) as specified by the manufacturer and recorded after 24, 48 and 72 h of incubation at 30 °C. Growth tests were performed in M17 medium (Oxoid, Hampshire, United Kingdom) supplemented with 1% cellobiose (cel-M17), 1% glucose (glu-M17) or 1% mannose (man-M17), using Microbiology Reader Analyser (Bioscreen C; Oy Growth Curves Ab Ltd, Helsinki, Finland). Optical density at 600 nm was monitored during 72 h of incubation at 30 °C in 1-h intervals. Assays were performed in triplicate. Exponential growth rates were determined as previously described27. The ability to use mannose as a single carbon source was determined by growing bacteria in CDM medium supplemented with 1% mannose (man-CDM).
Recombinant pNZ8037 plasmid with the ptnCD genes has been obtained previously7, whereas the one with the manCD genes was created in this study by ligation of the pNZ8037 vector with a PCR product containing the manCD genes, amplified with primers complmanCDfor/rev and digested with NcoI and XhoI. These recombinant pNZ8037 plasmids carrying either ptnCD or manCD genes were transformed into E. coli EC1000 resulting in the E. coli B529a or E. coli B530a strains, respectively. In the next step, recombinant pNZ8037 plasmids with the ptnCD or manCD genes were isolated using PureYieldTM plasmid mini-prep system (Promega, Fitchburg, Wi, United States) and used as template in site-directed mutagenesis, which was performed according to the Cornell iGEM 2012 Protocol (http://2012.igem.org/wiki/images/a/a5/Site_Directed_Mutagenesis.pdf). In order to introduce point mutations Pro102His or Ala104Val into ptnC, mutagenic primers ptnCPro102Hisfor/rev or ptnCAla104Valfor/rev, respectively, were used. To introduce respective point mutations into ptnD, the mutagenic primers ptnDPro108Serfor/rev, ptnDThr120Ilefor/rev or ptnDGly244Valfor/rev, were used. To introduce the Pro126His point mutation into manD, mutagenic primers manDPro126Hisfor/rev were used. All primer sequences are shown in Table S1 in the Supplementary File.
GarQ has a relatively wide activity spectrum
Although the GarQ activity spectrum has been examined previously by others19, in this study we reexamined it using a larger panel of microorganisms. GarQ showed a wide inhibitory spectrum being active against all tested bacterial species from the genera Carnobacterium, Enterococcus, Lactococcus, Leuconostoc and Listeria (Table 1). In the case of the Lactobacillus and Pediococcus genera, the susceptibility to GarQ was species-specific. Lactobacillus kunkeei and Pediococcus acidilactici showed resistance to GarQ, while the other Lactobacillus and Pediococcus spp. tested were sensitive to GarQ. Among the GarQ sensitive species tested, E. faecalis, Enterococcus faecium, Lactobacillus rhamnosus, L. garvieae and L. lactis strains were the most sensitive. GarQ showed no activity against Candida albicans, Pseudomonas aeruginosa, Salmonella typhimurium or all the tested species of the genera Bacillus, Campylobacter, Staphylococcus and Streptococcus.
Selection of L. garvieae IBB3403 GarQ-resistant mutants
To identify genes involved in the sensitivity to GarQ in target cells that could encode a GarQ receptor, we isolated resistant mutants of the highly sensitive strain L. garvieae IBB3403 by exposing the cells to increasing concentrations of GarQ on BHI-agar plates. Twenty-one independent spontaneous GarQ-resistant mutants were obtained at GarQ concentration of 1 mg/ml (mutants PW200 - PW220; Table 2). All these mutants were equally resistant to GarQ with the MIC value over 1024-fold higher than that of the parental L. garvieae IBB3403 (MIC = 0.098 µg/ml). After multiple passages without selective pressure of GarQ, no loss of resistance was observed indicating that the resistance was due to stable genetic mutations rather than an adaptation event.
GarQ-resistant mutants contain mutations in genes encoding Man-PTS
In order to identify the mutations in the genomes of the GarQ-resistant L. garvieae IBB3403 strains, DNA was isolated from nine randomly selected spontaneous mutants (PW200 - PW205, PW207, PW209 and PW210), sequenced and compared with the genome of parental GarQ-sensitive L. garvieae IBB3403. Single mutations were detected exclusively in the manABCD operon encoding Man-PTS (Table 2). Encouraged by this result, we performed direct sequencing of the manABCD operon in the remaining twelve GarQ-resistant mutants using specific primers. Altogether, among the twenty-one sequenced mutants, twelve harbored mutations in the manC gene encoding the IIC subunit, while the remaining nine mutants contained mutations in the manD gene coding for the IID subunit (Table 2). No mutation was found in the manAB gene encoding the cytoplasmic Man-PTS IIAB component, suggesting that this component is likely not required for the sensitivity to GarQ. The manCD mutations were of different types. Most caused premature truncation of the IIC or IID polypeptides, due to either nonsense or frameshift mutations in the manC or manD genes, and only three were missense mutations. Remarkably, in all three missense mutants the substituted amino acids were in the predicted outer regions of the transmembrane subunits IIC or IID. The PW202 mutant carried a C299 → A mutation in manC resulting in Pro100 → His substitution, while the PW203 and PW204 mutants carried single C368 → T and C331 → T mutations in manD resulting in Thr123 → Ile and Pro111 → Ser substitutions, respectively (Figs 1 and 2; Supplementary Fig. S1).
To assess whether the involvement of Man-PTS in GarQ sensitivity is species specific, we performed similar mutagenesis in the model LAB organism L. lactis IL1403. By the same approach, ten spontaneous L. lactis IL1403 mutants resistant to GarQ were obtained at 1 mg/ml GarQ (AT1 - AT10; Table 2) and all had the MIC values more than 1024-fold higher than the parental strain (0.024 µg/ml). Sequencing of their ptnABCD operons encoding Man-PTS revealed mutations in genes encoding the IIC or IID subunits and none in the gene coding for the IIAB component. The majority of the mutations were frameshift ones and none of the mutants harboured a missense mutation (Table 2).
Both IIC and IID subunits are indispensable for GarQ activity
The sequencing data strongly suggested that the observed GarQ resistance was due to the mutations in the ptnC or ptnD genes; however, a role of some unidentified mutations outside the ptnABCD operon could not be ruled out. To find out whether a compromised Man-PTS is sufficient to confer GarQ resistance we checked the GarQ sensitivity of an L. lactis strain missing the entire ptnABCD operon, L. lactis B4647. This ptnABCD-deletion strain had a MIC value ca. 1024-fold higher than the wild-type L. lactis IL1403, indicating full GarQ resistance. To identify the Man-PTS subunit(s) required for GarQ sensitivity, we performed a complementation test with different combinations of the three genes comprising the ptnABCD operon7 (Supplementary Table S1). As expected, the complete ptnABCD operon complemented the L. lactis B464 defect and restored GarQ sensitivity. No single gene (ptnAB, ptnC, or ptnD) alone was able to confer GarQ sensitivity, but two genes combined, ptnC and ptnD, were sufficient to cause GarQ sensitivity similar to that of a wild-type strain (Fig. 3). Altogether, these results clearly show that both IIC and IID subunits are required and sufficient for sensitivity to GarQ, whereas IIAB is not.
Garvicin Q structure, its similarity to other Man-PTS-binding bacteriocins and inhibitory spectrum
Man-PTS has been shown to be the receptor for IIa pediocin-like and two IId LcnA-like bacteriocins7, hence, we compared the GarQ amino acid sequence with these bacteriocins, to assess their relatedness. For this comparison we chose 2–3 members of each of four pediocins-like groups. Leader peptides of GarQ and pediocins showed almost no similarity (Fig. 4a). Mature peptides of GarQ and three group III pediocins - carnobacteriocin BM1, curvacin A and enterocin P showed some similarity at their C-terminal end (such as conservation of the GWxSxxAG motif) but mature peptides of GarQ and pediocins of the other tested subclasses showed almost no similarity (Fig. 4a). In contrast, the leader and mature peptides of GarQ and LcnA-like bacteriocins are more similar to each other, especially between the mature peptides of GarQ and LcnA (Fig. 4b).
A comparison of the activity spectra of the Man-PTS-targeting bacteriocins showed that GarQ, with its broad spectrum, is clearly distinct from the others (Table 1) since pediocin-like and LcnA-like bacteriocins are only active against Listeria spp. and L. lactis, respectively18. Since no information was available on the activity of the pediocin-like bacteriocins and LcnA against L. garvieae strains, we assayed the sensitivity of L. garvieae IBB3403 to sakacin A, pediocin PA1 and LcnA. These three bacteriocins showed no activity against L. garvieae IBB3403. Such specific inhibitory spectra suggest distinct modes of interaction of GarQ, LcnA and pediocin-like bacteriocins with their Man-PTS targets.
Next, based on partial templates available from Protein Data Bank (PDB), the secondary structure of GarQ was predicted to have an unstructured N-termini, whereas the C-terminal part was α-helical. Moreover, protein sequence homology searches revealed that the 30 amino acids sequence of GarQ C-terminal part shares around 50% identity and 69% similarity with sodium:proline solute transporter.
Sugar fermentation pattern of GarQ-resistant mutants depends on the nature of mutation
Given that GarQ targets a sugar transport system, we undertook to examine how the acquisition of resistance to GarQ affected the bacterial sugar metabolism. We profiled sugar utilization by selected L. garviaeae GarQ-resistant mutants containing missense or nonsense mutations in manCD. For the 49 sugars tested, the only specific defect was the inability of the nonsense mutant strains PW205 and PW210 to ferment mannose; their glucose metabolism remained intact.
To investigate the sugar metabolism of the GarQ-resistant mutants in more detail, their growth kinetics was determined in M17 medium supplemented with the Man-PTS-transported glucose or mannose, or with cellobiose not using Man-PTS for transport. In cel-M17, all mutants grew with the same kinetics as the wild-type strain (data not shown), whereas in glu- and man-M17 strain- or sugar-dependent growth differences were detected (Fig. 5). In glu-M17, all GarQ-resistant mutants exhibited slightly lower growth rates (between 0.480 h−1 for PW203 and 0.287 h−1 for PW205) than the wild-type L. garviaeae IBB3403 strain (0.562 h−1). In man-M17, mutants with missense mutations in manD (L. garviaeae PW203 and PW204) exhibited no significant differences in growth rate, while the PW202 mutant with a missense mutation in manC displayed a slightly lower growth rate (0.221 h−1) than the wild-type (0.462 h−1). The most significant decrease of the growth rate (0.042 h−1) and the final optical density was observed for the PW205 mutant harboring a nonsense mutation in manD (Fig. 5). Its growth was equal to the residual growth (in M17 medium without any sugar), indicating a total inability to metabolize mannose. Further tests to assess the ability to grow on man-CDM-agar plates of the remaining GarQ-resistant mutants confirmed the mannose-negative phenotype of the nonsense and frameshift mutants and mannose-positive of the missense ones (Table 2).
Resistance of bacterial species to GarQ is connected with their inability to utilize mannose
To determine whether species resistant to GarQ (Table 1) harbor genes encoding Man-PTS, we inspected their genomes available in the GenBank database. No genes encoding phylogenetic group I Man-PTSs were found in the genomes of Bacillus cereus, Bacillus subtilis, Campylobacter coli, Campylobacter jejuni, C. albicans, L. kunkeei, P. aeruginosa, S. typhimurium and Staphylococcus spp., which explains their resistance to GarQ (Table 1). In contrast, the genomes of P. acidilactici, and Streptococcus spp. carried entire operons encoding phylogenetic group I Man-PTSs, despite these species being resistant to GarQ. To estimate whether these Man-PTSs are functional, we tested these bacteria for growth on man-CDM. None of the GarQ-resistant species could grow on the man-CDM medium (Table 1) indicating that even the presence of an operon encoding a putative Man-PTS does not enable the bacteria to utilize mannose as a sole carbon source. This suggests that the putative Man-PTS-encoding operons were not functional, albeit one could envisage a situation where the bacterium would take up mannose using Man-PTS but would be then unable to metabolize it.
We also determined that species sensitive to GarQ harbored genes encoding phylogenetic group I Man-PTSs and manifested mannose-positive phenotype (Table 1). Taken together these results indicate a direct dependency between species’ ability to use mannose as a carbon source (functional or non-functional Man-PTS) and their sensitivity to GarQ.
Specific amino acids of Man-PTS are required for sensitivity to GarQ
Approach described in a previous study7 showed by immuno-precipitation a direct interaction between the Man-PTS complex and a bacteriocin upon its binding to target cells, however, was not conclusive about receptor specific amino acids that may be involved in a contact with a bacteriocin. Method developed in this study and a subsequent analysis of the isolated GarQ-resistant mutants pinpointed regions and individual amino acids of Man-PTS that are required for the L. lactis and L. garvieae sensitivity to GarQ and thus are presumably involved in the interaction with this bacteriocin. We based on the observation that, in contrast to the GarQ-resistant nonsense or frameshift mutants, the GarQ-resistant missense mutants maintained the mannose-positive phenotype. Thus, using a medium containing 1 mg/ml GarQ and mannose as a sole carbon source (man-CDM-GarQ) we selected L. lactis IL1403 and L. garvieae IBB3403 GarQ-resistant mutants that had a mannose-positive phenotype and harbored missense mutations in their respective Man-PTS-encoding operons. The obtained L. lactis IL1403 GarQ-resistant mutants were over 1024-fold more resistant than the parental strain, while their sensitivity to LcnA was unaffected, which further underlined the distinct modes of interaction of these two bacteriocins with Man-PTS. Among the L. garvieae isolates, five were over 1024-fold more resistant to GarQ than the parental strain and five – only ca. 16-fold. We then sequenced the relevant ptnABCD and manABCD operons from the ten obtained L. lactis IL1403 and ten L. garvieae IBB3403 GarQ-resistant mutants. Unexpectedly, all ten independent L. lactis mutants (viz. LLN1-LLN10) had the same C368 → A transition of a single nucleotide in ptnD resulting in the Pro123 → His substitution in the outer N-terminal region of the IID subunit of the Man-PTS (Table 2; Figs 1 and 2; Supplementary Fig. S1).
The ten independent L. garvieae GarQ-resistant mutants carried six different single mutations: one in manC resulting in the Ala102 → Val substitution (LGN9) and five in manD resulting in the Pro111 → Ser (LGN8, LGN10), Ala133 → Val (LGN2, LGN3), Trp256 → Leu (LGN4), Gly267 → Val (LGN6) or Gly301 → Val (LGN1, LGN5, LGN7) substitutions (Table 2; Figs 1 and 2; Supplementary Fig. S1). The substituted amino acids were localized either in the outer regions of Man-PTS subunit IID (L. garvieae LGN1, LGN4-LGN8, LGN10) or in the fourth (L. garvieae LGN9) or first (L. garvieae LGN2, LGN3) transmembrane regions of subunits IIC or IID, respectively (Figs 1 and 2; Supplementary Fig. S1). The Pro111 → Ser and Gly301 → Val mutants were over 1024-fold less sensitive to GarQ and the others 16-fold (Table 2).
At this point we also tested two L. lactis IL1403 LcnA-resistant mutants: L. lactis LMGT3726 with a missense mutation in ptnD resulting in the Pro115 → His substitution in IID, and L. lactis LMGT3727 with a missense mutation in ptnC resulting in the Gly184 → Asp substitution in IIC10. Both these mutants were still sensitive to GarQ, once again indicating that distinct amino acids of Man-PTS are responsible for the interaction with the GarQ and LcnA bacteriocins.
The amino acids altered by missense mutations in GarQ-resistant strains are conserved among GarQ-sensitive bacterial species
To examine whether the amino acids found to be required for Lactococcus spp. sensitivity to/binding GarQ are conserved among the other GarQ-sensitive species, we sequenced the operons encoding phylogenetic group I Man-PTSs of the eleven strains used in the activity spectrum assay. Additionally, we inspected sequences of eight phylogenetic group I Man-PTSs from genomes deposited in GenBank (their accession numbers are listed in Table S1 in the supplementary file). Six of the nine amino acids that were substituted in the GarQ-resistant mutants isolated in the present study (Ala102 in L. garvieae IIC, Pro111, Thr123, Ala133, Gly301 in L. garvieae IID and Pro123 in L. lactis IID) were strictly conserved in all the GarQ-sensitive species/strains inspected (Fig. 1; Supplementary Fig. S1). The other substituted amino acids (Pro100 in L. garvieae IIC and Trp256 and Gly267 in L. garvieae IID) were conserved only among the Lactococcus genus and L. garvieae strains, respectively. In other species, an alanine was found instead of Pro100 present in Lactococcus spp. The last two amino acids, Trp256 and Gly267, required for L. garvieae IBB3403 sensitivity to GarQ, had no equivalents in the other species as they are present in an additional extracellular loop of IID specific to L. garvieae (Fig. 2; Supplementary Fig. S1).
Since different amino acids were altered by missense mutations in GarQ-resistant mutants of L. garvieae IBB3403 and L. lactis IL1403 (Table 2), we tested whether mutating the same amino acids in these two species would make them insensitive to GarQ. Thus, by site-directed mutagenesis we introduced missense mutations that appeared by random mutagenesis in ptnABCD into manABCD and vice-versa, and expressed the mutated operons in L. lactis B464 with a full deletion of the ptnABCD operon. All the strains obtained by this procedure (Supplementary Table S1) turned out to be less sensitive to GarQ than the reference L. lactis B529 or L. lactis B530a strains indicating the all the substituted amino acids are required for GarQ toxicity, and presumably also for GarQ-Man-PTS interaction in the two Lactococcus species.
Pathogenic L. garvieae strains may cause bovine mastitis28 and lactococcosis, a hyperacute systemic disease that can occur in cultured or wild fish species29. Thus, bacteriocins produced by, and active against, L. garvieae strains show an applicative potential in the prevention or treatment of these infections. Besides, like other bacteriocins produced by Gram-positive bacteria, they can be used to combat certain infections caused by Gram-positive pathogens other than L. garvieae. To date, six bacteriocins produced by L. garvieae have been reported: garviecin L1-530, garvicin ML31, garvicin Q19, garvicin A32, garviecin LG3433 and garvicin KS34. However, receptors for most of these bacteriocins on target cells remain unknown. Here, we chose GarQ, which has a broad activity spectrum with genus or species-directed specificity. Importantly, GarQ is active against Listeria species infecting humans and animals, L. monocytogenes the etiological agent of listeriosis, and Listeria ivanovii causing gastroenteritis and bacteremia, which suggests its possible therapeutic use.
To identify the GarQ receptor, we used whole genome sequencing (WGS) of spontaneous GarQ-resistant mutants. Such an approach has been demonstrated to be appropriate for genotypic analysis of resistant mutants9,10,11. The consistent pattern of mutations identified in numerous independently derived spontaneous GarQ-resistant strains pointed to Man-PTS being required for susceptibility to GarQ. This conclusion was verified experimentally. Deletion of the operon encoding Man-PTS and subsequent phenotypical studies confirmed that a functional Man-PTS is essential for bacterial sensitivity to GarQ. Furthermore, complementation with genes encoding only the membrane components of Man-PTS (IIC and IID) confirmed that both these subunits are needed for GarQ sensitivity, whereas the cytoplasmic IIAB component is dispensable.
Man-PTS is a system indispensable for mannose uptake by a bacterial cell12. Phenotypic tests of the GarQ-resistant mutants obtained during this study revealed a strong correlation between the type of the mutation and the ability to metabolize mannose. Mutants with nonsense or frameshift mutations in genes encoding Man-PTS were no longer able to metabolize mannose, while those harboring missense mutations were still able to grow on mannose (Table 2). The deficiency of mannose metabolism in a select group of GarQ-resistant mutants suggests that their nonsense or frameshift mutations had a deleterious effect on Man-PTS structure, which deprived it of its crucial mannose-uptake function. On the other hand, the retained ability to utilize mannose by the missense GarQ-resistant mutants suggests that the amino acid substitutions in these mutants had no negative effect on the transmembrane structure of Man-PTS preserving its functionality related to sugar transport. In line with this observation is the mannose fermentation deficiency of all GarQ-resistant indicator species (Table 1), suggesting that in these bacteria Man-PTS-encoding operons are absent or non-functional. Indeed, we could not identify such operons in some of GarQ-resistant species’ genomes, while in other species a potential Man-PTS-encoding operon was clearly present. We believe that in the latter cases operons were non-functional as these cells were not only insensitive to GarQ but also unable to exploit mannose as a carbon source. The latter due to the fact that Man-PTS is the only permease that facilitates import of mannose into bacterial cells. Our comparative analyses of the man-PTS ribosome binding site (RBS) spacer length (the distance between the initiation codon and the RBS) revealed extended sequences (10–11 nucleotides) in GarQ-resistant and mannose-negative species, whereas shorter ones (7–9 nucleotides) in GarQ-sensitive and mannose-positive species. Thus, it is plausible that the occurrence of longer RBS spacers above man-PTS may be a factor that negatively affects the formation of the 30 S initiation complex leading to the severe reduction or lack of the Man-PTS proteins synthesis resulting in GarQ-resistant and mannose-negative phenotype. The efficient use of glucose by those strains was likely due to the fact that for glucose, in contrast to mannose, also other sugar transporting systems can be used.
A comparison of amino acid sequences and activity spectra of GarQ with other bacteriocins that use Man-PTS as a receptor on target cells revealed that GarQ shares no or weak similarity with these bacteriocins. It is known that pediocins are active only against strains containing region α in the Man-PTS IIC subunit and therefore they act against L. monocytogenes but not against L. lactis 18. Conversely, LcnA-like bacteriocins are only active against L. lactis strains18. Interestingly, GarQ targets both L. monocytogenes and L. lactis strains, and is also active against L. garvieae strains, which do not have a fully conserved region α in their Man-PTS IIC subunits. As we showed in this study, pediocin- and LcnA-like bacteriocins show no activity against L. garvieae strains. This suggests that GarQ must interact with its receptor via a mechanism distinct from the mechanisms by which other bacteriocins recognize Man-PTS. Further support for this hypothesis comes from the fact that the L. lactis IL1403 mutants with a missense mutation in the ptnD gene altering Pro123 in subunit IID, were fully resistant to GarQ but were still sensitive to LcnA. Reciprocally, two L. lactis IL1403 LcnA-resistant strains with missense mutations substituting Gly188 in IIC or Pro115 in IID subunit of Man-PTS were still sensitive to GarQ. These data clearly indicate that distinct amino acids of the Man-PTS subunits IIC and IID are engaged in the interactions with GarQ, LcnA and pediocin-like bacteriocins.
Moreover, we found that in the missense GarQ-resistant mutants, the level of resistance to GarQ varied from 16- to over 1024-fold compared to the wild-type lactococcal strains (Table 2). This difference depended on the localization of the amino acid substituted and may be explained by diversified binding affinities between GarQ and the specific IICD amino acids. Thus, it is tempting to propose that the amino acids whose substitutions caused the high resistance to GarQ (Pro100 in L. garvieae IIC, Thr123, Pro111 and Gly301 in L. garvieae IID, and Pro123 in L. lactis IID) are the main determinants of the GarQ binding. Other amino acids, substitutions of which only led to partial resistance to GarQ (Ala102 in L. garvieae IIC and Ala133, Trp256 and Gly267 in L. garvieae IID), likely have only a supporting role in the GarQ - Man-PTS interaction (Fig. 2). All these Man-PTS amino acids potentially interacting with GarQ seem to be easily accessible for the bacteriocin, as most of them are localized in predicted outer regions of Man-PTS, and the rest in transmembrane regions adjacent to the outer membrane face (Fig. 2).
In this study we confirmed that among Lactococcus species the same set of Man-PTS amino acids is necessary for sensitivity to/binding with GarQ. Moreover, these amino acids, with a single exception, are highly conserved also among other GarQ-sensitive species (Supplementary Fig. S1). This conservation suggests that in all GarQ-sensitive species the same amino acids of Man-PTS are involved in the interaction with GarQ.
It is tempting to speculate that among the GarQ-sensitive species, the N-terminal part of the bacteriocin initially interacts with, depending on the species, five or seven amino acids of the Man-PTS IID extracellular loops specified in this study. This in turn may enable interaction of the C-terminal α-helix-containing part of the bacteriocin with two IIC amino acids (proline and alanine). As these two amino acids are predicted to be transmembrane-localised, we can further assume that they are part of the IIC inner pore helix. Such GarQ-receptor interaction might trigger structural changes in Man-PTS arousing the IIC permease to open as a pore, thereby leading to leakage of solutes across the membrane, disruption of membrane integrity and finally cell death. We can also presume that due to the similarity of the GarQ C-terminus to transporter domain, GarQ molecules bound to IIC may form a stable pore, which, by its structure, might act to facilitate the leakage of solutes.
Altogether, our results show that the presence of phylogenetic group I Man-PTS determines the sensitivity of genera Carnobacterium, Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Listeria and Pediococcus to garvicin Q. To the best of our knowledge, GarQ is the first bacteriocin outside the pediocin- or LcnA-like bacteriocins shown to interact with Man-PTS. It is also the first bacteriocin targeting Man-PTSs from both L. monocytogenes and L. lactis. GarQ interacts with Man-PTS through a new, previously unknown binding pattern involving specific amino acids located in the extracellular loops and N-terminal end of the IID subunit and in a transmembrane region of the subunit IIC. This study presents a deeper insight into the important role of Man-PTS as the receptor for multiple unrelated bacteriocins. Our further studies will focus on understanding how the distinct bacteriocins recognize the same receptor and how Man-PTS and bacteriocins co-evolved to allow such promiscuity35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51.
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This work was supported by grant no. 2013/08/M/NZ9/01025 from the National Science Centre.
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Tymoszewska, A., Diep, D.B., Wirtek, P. et al. The Non-Lantibiotic Bacteriocin Garvicin Q Targets Man-PTS in a Broad Spectrum of Sensitive Bacterial Genera. Sci Rep 7, 8359 (2017). https://doi.org/10.1038/s41598-017-09102-7
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