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The image data used to generate the correction matrices for the 24-well plate adapter are available upon request. The calculated correction matrices are available online at https://www.github.com/WeberSynBioLab/optoConfig-96.
optoConfig-96 is available at https://www.github.com/WeberSynBioLab/optoConfig-96, along with the most up-to-date version of the user guide (Supplementary Manual).
Bugaj, L. J. et al. Cancer mutations and targeted drugs can disrupt dynamic signal encoding by the Ras-Erk pathway. Science 361, eaao3048 (2018).
de Beco, S. et al. Optogenetic dissection of Rac1 and Cdc42 gradient shaping. Nat. Commun. 9, 1–13 (2018).
Zhou, X. X., Fan, L. Z., Li, P., Shen, K. & Lin, M. Z. Optical control of cell signaling by single-chain photoswitchable kinases. Science 355, 836–842 (2017).
Yousefi, O. S. et al. Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor. eLife 8, e42475 (2019).
Tischer, D. K. & Weiner, O. D. Light-based tuning of ligand half-life supports kinetic proofreading model of T cell signaling. eLife 8, e42498 (2019).
Zhang, P. et al. Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology. eLife 8, e39578 (2019).
Kolar, K., Knobloch, C., Stork, H., Žnidarič, M. & Weber, W. OptoBase: a web platform for molecular optogenetics. ACS Synth. Biol. 7, 1825–1828 (2018).
Bugaj, L. J. & Lim, W. A. High-throughput multicolor optogenetics in microwell plates. Nat. Protoc. 14, 2205–2228 (2019).
We thank J. Schmidt, D. Renz and L. Breh (technical workshop of the Faculty of Biology, University of Freiburg, Germany) for assembling the optoPlates-96 and 3D printing of the 24-well plate adapter. We also thank A. Fischer, C. Jerez-Longres, L. Lataster, H. Mohsenin and N. Urban for testing optoConfig-96. This work was supported in part by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy CIBSS–EXC-2189, Project ID 390939984. This work was supported in part by the Excellence Initiative of the Deutsche Forschungsgemeinschaft (GSC–4, Spemann Graduate School) and in part by the Ministry for Science, Research and Arts of the State of Baden-Wuerttemberg.
The authors declare no competing interests.
Peer review information Nature Protocols thanks Andreas Möglich and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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Thomas, O.S., Hörner, M. & Weber, W. A graphical user interface to design high-throughput optogenetic experiments with the optoPlate-96. Nat Protoc (2020). https://doi.org/10.1038/s41596-020-0349-x