A graphical user interface to design high-throughput optogenetic experiments with the optoPlate-96

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Fig. 1: Overview of the optoConfig-96 workflow.

Data availability

The image data used to generate the correction matrices for the 24-well plate adapter are available upon request. The calculated correction matrices are available online at https://www.github.com/WeberSynBioLab/optoConfig-96.

Code availability

optoConfig-96 is available at https://www.github.com/WeberSynBioLab/optoConfig-96, along with the most up-to-date version of the user guide (Supplementary Manual).


  1. 1.

    Bugaj, L. J. et al. Cancer mutations and targeted drugs can disrupt dynamic signal encoding by the Ras-Erk pathway. Science 361, eaao3048 (2018).

    Article  Google Scholar 

  2. 2.

    de Beco, S. et al. Optogenetic dissection of Rac1 and Cdc42 gradient shaping. Nat. Commun. 9, 1–13 (2018).

    Article  Google Scholar 

  3. 3.

    Zhou, X. X., Fan, L. Z., Li, P., Shen, K. & Lin, M. Z. Optical control of cell signaling by single-chain photoswitchable kinases. Science 355, 836–842 (2017).

    Article  Google Scholar 

  4. 4.

    Yousefi, O. S. et al. Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor. eLife 8, e42475 (2019).

    Article  Google Scholar 

  5. 5.

    Tischer, D. K. & Weiner, O. D. Light-based tuning of ligand half-life supports kinetic proofreading model of T cell signaling. eLife 8, e42498 (2019).

    Article  Google Scholar 

  6. 6.

    Zhang, P. et al. Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology. eLife 8, e39578 (2019).

    Article  Google Scholar 

  7. 7.

    Kolar, K., Knobloch, C., Stork, H., Žnidarič, M. & Weber, W. OptoBase: a web platform for molecular optogenetics. ACS Synth. Biol. 7, 1825–1828 (2018).

    CAS  Article  Google Scholar 

  8. 8.

    Bugaj, L. J. & Lim, W. A. High-throughput multicolor optogenetics in microwell plates. Nat. Protoc. 14, 2205–2228 (2019).

    CAS  Article  Google Scholar 

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We thank J. Schmidt, D. Renz and L. Breh (technical workshop of the Faculty of Biology, University of Freiburg, Germany) for assembling the optoPlates-96 and 3D printing of the 24-well plate adapter. We also thank A. Fischer, C. Jerez-Longres, L. Lataster, H. Mohsenin and N. Urban for testing optoConfig-96. This work was supported in part by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy CIBSS–EXC-2189, Project ID 390939984. This work was supported in part by the Excellence Initiative of the Deutsche Forschungsgemeinschaft (GSC–4, Spemann Graduate School) and in part by the Ministry for Science, Research and Arts of the State of Baden-Wuerttemberg.

Author information




O.S.T., M.H. and W.W. conceptualized optoConfig-96. O.S.T. wrote the software. M.H. designed and characterized the 24-well plate adapter. M.H. and W.W. supervised the project. O.S.T., M.H. and W.W. wrote and edited the manuscript.

Corresponding authors

Correspondence to Maximilian Hörner or Wilfried Weber.

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The authors declare no competing interests.

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Peer review information Nature Protocols thanks Andreas Möglich and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.

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Supplementary information

Supplementary Information

Supplementary Notes 1 and 2, Supplementary Manual and Supplementary References

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Thomas, O.S., Hörner, M. & Weber, W. A graphical user interface to design high-throughput optogenetic experiments with the optoPlate-96. Nat Protoc (2020). https://doi.org/10.1038/s41596-020-0349-x

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