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The data are available at SRA under project number PRJNA494935.
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We thank R. Kurita and Y. Nakamura (RIKEN BioResource Center, Tsukuba, Japan) for sharing HUDEP-2 cells. L.P. is supported by NHGRI Career Development Award R00 HG008399 and CEGS RM1HG009490. D.E.B. is support by NIH R03 DK109232, NIH DP2 OD022716, NIH P01 HL032262, the Burroughs Wellcome Fund, and the Doris Duke Charitable Foundation. J.K.J. is supported by NIH R35 GM118158, NIH RM1 HG009490, and the Desmond and Ann Heathwood MGH Research Scholar Award. M.C.C. is supported by NIDDK Award F30-DK103359. J.M.E. is supported by NIH NHGRI 1K99HG009917-01 and the Harvard Society of Fellows.
Integrated supplementary information
(a) An overview of the BCL11A CRISPR–Cas9 enhancer dissection tiling screen. (b) Zoom-in panels of DHS +55, +58, +62, and BCL11A exon 2 to highlight critical regions identified by CRISPR-SURF. All significant regions identified with FDR < 0.05. All panels are shown at same scale.
(a) An overview of the MYC CRISPRi enhancer discovery tiling screen. (b) Zoom-in panels of MYC TSS, e1–e7, and r1 (regions identified in ref. 2) along with newly identified regions by CRISPR-SURF (SURF1 and SURF2). All significant regions identified with FDR < 0.05. All panels are shown at same scale.
(a) An overview of the IL2RA CRISPRa enhancer discovery tiling screen. (b) Zoom-in panels of IL2RA TSS and CaREs 1–6 (regions identified in ref. 3) along with regions newly identified by CRISPR-SURF (SURF3 and SURF4). All significant regions identified with FDR < 0.05. All panels are shown at same scale.
Supplementary Figure 4 CRISPR-SURF analysis of parallel CRISPRi and CRISPR–Cas9 DHS tiling screens targeted to the BCL11A locus.
(a) An overview of the BCL11A CRISPRi and CRISPR–Cas9 DHS tiling screens. (b) Shown are zoom-in panels of BCL11A exon 2 and common significant regions (FDR < 0.05) between the CRISPRi and CRISPR–Cas9 tiling screens as determined by CRISPR-SURF.