Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Experimental microbial dysbiosis does not promote disease progression in SIV-infected macaques

Abstract

Intestinal microbial dysbiosis has been described in individuals with an HIV-1 infection and may underlie persistent inflammation in chronic infection, thereby contributing to disease progression. Herein, we induced an HIV-1-like intestinal dysbiosis in rhesus macaques (Macaca mulatta) with vancomycin treatment and assessed the contribution of dysbiosis to SIV disease progression. Dysbiotic and control animals had similar disease progression, indicating that intestinal microbial dysbiosis similar to that observed in individuals with HIV is not sufficient to accelerate untreated lentiviral disease progression.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Fig. 1: Vancomycin treatment promotes dysbiosis in SIV-infected RMs.
Fig. 2: SIV disease progression is unaffected by vancomycin-induced intestinal dysbiosis.

References

  1. 1.

    Ortiz, A. M. & Brenchley, J. M. Curr. Opin. HIV AIDS 13, 15–21 (2018).

    CAS  Article  Google Scholar 

  2. 2.

    Brenchley, J. M., Silvestri, G. & Douek, D. C. Immunity 32, 737–742 (2010).

    CAS  Article  Google Scholar 

  3. 3.

    Noguera-Julian, M. et al. EBioMedicine 5, 135–146 (2016).

    Article  Google Scholar 

  4. 4.

    Policicchio, B. B., Pandrea, I. & Apetrei, C. Front. Immunol. 7, 12 (2016).

    Article  Google Scholar 

  5. 5.

    Mudd, J. C. & Brenchley, J. M. J. Infect. Dis. 214 Suppl 2, S58–S66 (2016).

    Article  Google Scholar 

  6. 6.

    Ivanov, I. I. et al. Cell 139, 485–498 (2009).

    CAS  Article  Google Scholar 

  7. 7.

    Kelly, C. J. et al. Cell Host Microbe 17, 662–671 (2015).

    CAS  Article  Google Scholar 

  8. 8.

    Day, C. L. et al. Nature 443, 350–354 (2006).

    CAS  Article  Google Scholar 

  9. 9.

    Betts, M. R. et al. Blood 107, 4781–4789 (2006).

    CAS  Article  Google Scholar 

  10. 10.

    McKenna, P. et al. PLoS Pathog. 4, e20 (2008).

    Article  Google Scholar 

  11. 11.

    Gosmann, C. et al. Immunity 46, 29–37 (2017).

    CAS  Article  Google Scholar 

  12. 12.

    d’Ettorre, G. et al. Immun. Inflamm. Dis. 5, 244–260 (2017).

    Article  Google Scholar 

  13. 13.

    Vujkovic-Cvijin, I. et al. Gut Microbes 8, 440–450 (2017).

    Article  Google Scholar 

  14. 14.

    Hager, C. L. & Ghannoum, M. A. Curr. Opin. HIV AIDS 13, 69–72 (2018).

    CAS  Article  Google Scholar 

  15. 15.

    Ortiz, A. M. et al. Mucosal Immunol. 9, 458–467 (2016).

    CAS  Article  Google Scholar 

  16. 16.

    Caporaso, J. G. et al. Nat. Methods 7, 335–336 (2010).

    CAS  Article  Google Scholar 

  17. 17.

    Langille, M. G. et al. Nat. Biotechnol. 31, 814–821 (2013).

    CAS  Article  Google Scholar 

  18. 18.

    Estes, J. D. et al. PLoS Pathog. 6, e1001052 (2010).

    Article  Google Scholar 

  19. 19.

    Hao, X. P. et al. Nat. Commun. 6, 8020 (2015).

    CAS  Article  Google Scholar 

  20. 20.

    Brenchley, J. M. et al. Blood 120, 4172–4181 (2012).

    CAS  Article  Google Scholar 

  21. 21.

    Segata, N. et al. Genome Biol. 12, R60 (2011).

    Article  Google Scholar 

  22. 22.

    Parks, D. H., Tyson, G. W., Hugenholtz, P. & Beiko, R. G. Bioinformatics 30, 3123–3124 (2014).

    CAS  Article  Google Scholar 

Download references

Acknowledgements

We would like to acknowledge H. Kendall, J. Swerczek, R. Herbert, and all the veterinary staff at the NIH animal center. We would like to thank M. Quiñones, J. Davis, S. Sen and G. Trinchieri, and the NIAID Microbiome Program for technical and analytical assistance. Funding for this study was provided in part by the Division of Intramural Research/NIAID/NIH, by the Oregon National Primate Research Center NIH grant award P51OD011092, and with federal funds from the National Cancer Institute, NIH, under Contract No. HHSN261200800001E. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services (DHHS), nor does the mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.

Author information

Affiliations

Authors

Contributions

J.M.B. conceived the project. A.M.O. and J.M.B. designed the project and wrote the manuscript. A.M.O., J.K.F., S.R.D., C.E.C.S., S.H.L., M.E.L., O.S., C.L.V., J.C.M., L.J., K.B.-S., J.D.E. and J.M.B. performed experiments. A.M.O., J.K.F., S.R.D., I.V.-C., S.H.L., Y.B., J.D.E. and J.M.B. analyzed data.

Corresponding author

Correspondence to Jason M. Brenchley.

Ethics declarations

Competing interests

The authors declare no competing interests.

Additional information

Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–8 and Supplementary Table 1

Reporting Summary

Supplementary Dataset 1

LEFSE returned results

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Ortiz, A.M., Flynn, J.K., DiNapoli, S.R. et al. Experimental microbial dysbiosis does not promote disease progression in SIV-infected macaques. Nat Med 24, 1313–1316 (2018). https://doi.org/10.1038/s41591-018-0132-5

Download citation

Further reading

  • A Summary of the Fifth Annual Virology Education HIV Microbiome Workshop

    • Scott Sherrill-Mix
    • , Kaleigh Connors
    • , Grace M. Aldrovandi
    • , Jason M. Brenchley
    • , Charles Boucher
    • , Frederic D. Bushman
    • , Ronald G. Collman
    • , Satya Dandekar
    • , Nichole R. Klatt
    • , Laurel A. Lagenaur
    • , Roger Paredes
    • , Gilda Tachedjian
    • , Jim A. Turpin
    • , Alan L. Landay
    •  & Mimi Ghosh

    AIDS Research and Human Retroviruses (2020)

  • Diversity and Complexity of the Large Surface Protein Family in the Compacted Genomes of MultiplePneumocystisSpecies

    • Liang Ma
    • , Zehua Chen
    • , Da Wei Huang
    • , Ousmane H. Cissé
    • , Jamie L. Rothenburger
    • , Alice Latinne
    • , Lisa Bishop
    • , Robert Blair
    • , Jason M. Brenchley
    • , Magali Chabé
    • , Xilong Deng
    • , Vanessa Hirsch
    • , Rebekah Keesler
    • , Geetha Kutty
    • , Yueqin Liu
    • , Daniel Margolis
    • , Serge Morand
    • , Bapi Pahar
    • , Li Peng
    • , Koen K. A. Van Rompay
    • , Xiaohong Song
    • , Jun Song
    • , Antti Sukura
    • , Sabrina Thapar
    • , Honghui Wang
    • , Christiane Weissenbacher-Lang
    • , Jie Xu
    • , Chao-Hung Lee
    • , Claire Jardine
    • , Richard A. Lempicki
    • , Melanie T. Cushion
    • , Christina A. Cuomo
    • , Joseph A. Kovacs
    •  & Louis M. Weiss

    mBio (2020)

  • Impact of Acute HIV Infection and Early Antiretroviral Therapy on the Human Gut Microbiome

    • Ornella Sortino
    • , Nittaya Phanuphak
    • , Alexandra Schuetz
    • , Alexandra M Ortiz
    • , Nitiya Chomchey
    • , Yasmine Belkaid
    • , Jacquice Davis
    • , Harry A Mystakelis
    • , Mariam Quiñones
    • , Claire Deleage
    • , Brian Ingram
    • , Rungsun Rerknimitr
    • , Suteeraporn Pinyakorn
    • , Adam Rupert
    • , Merlin L Robb
    • , Jintanat Ananworanich
    • , Jason Brenchley
    • , Irini Sereti
    • , Nipat Teeratakulpisarn
    • , Supanit Pattanachaiwit
    • , Mark de Souza
    • , James Fletcher
    • , Eugene Kroon
    • , Ponpen Tantivitayakul
    • , Duanghathai Suttichom
    • , Somprartthana Rattanamanee
    • , Kultida Poltavee
    • , Jintana Intasan
    • , Tassanee Luekasemsuk
    • , Hathairat Savadsuk
    • , Somporn Tipsuk
    • , Suwanna Puttamsawin
    • , Khunthalee Benjapornpong
    • , Nisakorn Ratnaratorn
    • , Patcharin Eamyoung
    • , Sasiwimol Ubolyam
    • , Robert O’Connell
    • , Siriwat Akapirat
    • , Bessara Nuntapinit
    • , Nantana Tantibul
    • , Nampueng Churikanont
    • , Saowanit Getchalarat
    • , Sandhya Vasan
    • , Rapee Trichavaroj
    • , Chayada Sajiaweerawan
    • , Yuwadee Phuang-Ngern
    • , Surat Jongrakthaitae
    • , Suchada Sukhumvittaya
    • , Putida Saetun
    • , Weerawan Chuenarom
    • , Nelson Michael
    • , Ellen Turk
    • , Corinne McCullough
    • , Oratai Butterworth
    •  & Mark Milazzo

    Open Forum Infectious Diseases (2020)

  • HIV and the Gut Microbiota: Composition, Consequences, and Avenues for Amelioration

    • Ivan Vujkovic-Cvijin
    •  & Ma Somsouk

    Current HIV/AIDS Reports (2019)

  • Increased mucosal neutrophil survival is associated with altered microbiota in HIV infection

    • Tiffany Hensley-McBain
    • , Michael C. Wu
    • , Jennifer A. Manuzak
    • , Ryan K. Cheu
    • , Andrew Gustin
    • , Connor B. Driscoll
    • , Alexander S. Zevin
    • , Charlene J. Miller
    • , Ernesto Coronado
    • , Elise Smith
    • , Jean Chang
    • , Michael Gale
    • , Ma Somsouk
    • , Adam D. Burgener
    • , Peter W. Hunt
    • , Thomas J. Hope
    • , Ann C. Collier
    • , Nichole R. Klatt
    •  & Daniel C. Douek

    PLOS Pathogens (2019)

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing