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Reply to: The role of recruitment versus training in influenza-induced lasting changes to alveolar macrophage function

Nature Immunology volume 24, pages 1642–1644 (2023)Cite this article

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The Original Article was published on 28 August 2023

replying to C. S. Iliakis et al. Nature Immunology https://doi.org/10.1038/s41590-023-01602-1 (2023)

With great interest, we read the comments by Iliakis et al.1 related to our article published in Nature Immunology entitled ‘Influenza-trained mucosal-resident alveolar macrophages confer long-term antitumor immunity in the lungs’ (ref. 2). Li et al. show in a recent study that intratracheal influenza A virus (IAV) infection in mice induces bone marrow monocyte-derived alveolar macrophages (AMs) (BMoAMs) characterized by lower sialic acid-binding immunoglobulin-like lectin F (Siglec-F) expression than that of embryonic- or fetal monocyte-derived AMs (FeMoAMs)3, and Iliakis et al. argue that elevated immunoreactivity in AMs after intranasal IAV infection in our study is due to recruited BMoAMs having what they call a ‘monocyte legacy’ instead of training of FeMoAMs1. Iliakis et al. further speculate that BMoAMs were excluded in our flow cytometry analysis, as these cells resemble interstitial macrophages (IMs) in the Siglec-F–Ly6C dot plot (Extended Data Fig. 1a in ref. 2).

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Fig. 1: IAV-trained AMs develop independently of circulating monocytes.

References

  1. Iliakis, C. The role of recruitment versus training in influenza-induced lasting changes to alveolar macrophage function. Nat. Immunol. https://doi.org/10.1038/s41590-023-01602-1 (2023).

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Authors and Affiliations

  1. Institute of Immunology and Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China

    Tao Wang, Jinjing Zhang, Lu Wang, Yanling Wang, Ying Li & Yushi Yao

  2. Liangzhu Laboratory, Zhejiang University Medical Center, Hangzhou, China

    Tao Wang, Jinjing Zhang, Lu Wang, Yanling Wang, Ying Li & Yushi Yao

Authors
  1. Tao Wang
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  2. Jinjing Zhang
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  3. Lu Wang
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  4. Yanling Wang
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  5. Ying Li
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  6. Yushi Yao
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Contributions

T.W. and Y.Y. conceived and designed the study. T.W., J.Z., L.W., Y.W., Y.L. and Y.Y. performed experiments. T.W., L.W. and Y.Y. analyzed data. T.W. and Y.Y. wrote the paper.

Corresponding author

Correspondence to Yushi Yao.

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The authors declare no competing interests.

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Nature Immunology thanks Maziar Divangahi and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Primary Handling Editor: N. Bernard, in collaboration with the Nature Immunology team.

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Wang, T., Zhang, J., Wang, L. et al. Reply to: The role of recruitment versus training in influenza-induced lasting changes to alveolar macrophage function. Nat Immunol 24, 1642–1644 (2023). https://doi.org/10.1038/s41590-023-01603-0

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