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Spatiotemporal dynamics of focal adhesion kinase

Nature Chemical Biology volume 19, pages 1444–1445 (2023)Cite this article

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We designed FAK-SPARK, a fluorescent reporter of focal adhesion kinase (FAK) activity based on phase separation. FAK-SPARK revealed polarized FAK activity within single focal adhesions in the leading edges of migrating cells. By combining FAK-SPARK with DNA tension probes, we showed that FAK activity is proportional to the tension strength.

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Fig. 1: FAK activity is polarized at the distal tip of newly formed focal adhesions in the leading edges of migrating cells.

References

  1. Murphy, J. M., Park, H. & Lim, S.-T. S. FAK and Pyk2 in disease. Front. Biol. 11, 1–9 (2016). A review article discussing FAK in diseases.

    Article  CAS  Google Scholar 

  2. Shu, X. Imaging dynamic cell signaling in vivo with new classes of fluorescent reporters. Curr. Opin. Chem. Biol. 54, 1–9 (2020). A review article discussing various new physical principle-based signaling sensors.

    Article  CAS  PubMed  Google Scholar 

  3. Zhang, Q. et al. Visualizing dynamics of cell signaling in vivo with a phase separation-based kinase reporter. Mol. Cell 69, 347 (2018). A research article developing SPARK sensors of serine/threonine kinases.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Zhang, Y., Ge, C., Zhu, C. & Salaita, K. DNA-based digital tension probes reveal integrin forces during early cell adhesion. Nat. Commun. 5, 5167 (2014). A research article developing DNA-based tension probes.

    Article  CAS  PubMed  Google Scholar 

  5. Lu, P., Weaver, V. M. & Werb, Z. The extracellular matrix: a dynamic niche in cancer progression. J. Cell Biol. 196, 395–406 (2012). A review article discussing extracellular matrix and cancer.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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This is a summary of: Li, X. et al. Polarized focal adhesion kinase activity within a focal adhesion during cell migration. Nat. Chem. Biol. https://doi.org/10.1038/s41589-023-01353-y (2023).

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Spatiotemporal dynamics of focal adhesion kinase. Nat Chem Biol 19, 1444–1445 (2023). https://doi.org/10.1038/s41589-023-01371-w

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