Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Genomics

Decoding gene regulation with CRISPR perturbations

Nature Biotechnology (2024)Cite this article

Subjects

Two CRISPR tools for combinatorial genetic perturbations reveal gene regulatory networks.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Two novel CRISPR tools to map genomic regulatory sequences.

References

  1. Pacalin, N. M. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02213-3 (2024).

    Article  Google Scholar 

  2. Hsiung, C. C.-S. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02224-0 (2024).

    Article  Google Scholar 

  3. Henikoff, S. & Ahmad, K. Protein Sci. 31, e4458 (2022).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Melnikov, A. et al. Nat. Biotechnol. 30, 271–277 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Arnold, C. D. et al. Science 339, 1074–1077 (2013).

    Article  CAS  PubMed  Google Scholar 

  6. Fulco, C. P. et al. Nat. Genet. 51, 1664–1669 (2019).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Najm, F. J. et al. Nat. Biotechnol. 36, 179–189 (2018).

    Article  CAS  PubMed  Google Scholar 

  8. Boettcher, M. et al. Nat. Biotechnol. 36, 170–178 (2018).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Gasperini, M. et al. Cell 176, 377–390.e19 (2019).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology & UCSF Diabetes Center, University of California, San Francisco, San Francisco, CA, USA

    Stefan Oberlin & Michael T. McManus

Authors
  1. Stefan Oberlin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michael T. McManus
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Michael T. McManus.

Ethics declarations

Competing interests

The authors declare no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Oberlin, S., McManus, M.T. Decoding gene regulation with CRISPR perturbations. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02222-2

Download citation

  • Published:

  • DOI: https://doi.org/10.1038/s41587-024-02222-2

Search

Advanced search

Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research