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Reply to: Methodological concerns and lack of evidence for single-synapse RNA-seq

Nature Biotechnology volume 41, pages 1225–1228 (2023)Cite this article

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The Original Article was published on 27 July 2023

replying to B. D. Hobson and E. Herzog Nature Biotechnology https://doi.org/10.1038/s41587-023-01877-7 (2023)

The commentary by B. D. Hobson and E. Herzog1 has raised major criticism about our approach to isolating synaptosomes. We believe that the two authors have misunderstood many aspects of our experimental design; furthermore, their commentary ignores our control experiments for validation of the profiled synaptome. As a result, we believe that the criticisms raised by Hobson and Herzog cannot be justified, and we respond below in detail.

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Fig. 1: Consistent pseudo-bulk synaptic transcriptomes observed between sorting-based and gradient centrifugation-based approaches.

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Author information

Authors and Affiliations

  1. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA

    Muchun Niu & Chenghang Zong

  2. Integrative Molecular and Biomedical Sciences Graduate Program, Baylor College of Medicine, Houston, TX, USA

    Muchun Niu

  3. Dan L. Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX, USA

    Chenghang Zong

  4. McNair Medical Institute, Baylor College of Medicine, Houston, TX, USA

    Chenghang Zong

Authors
  1. Muchun Niu
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Contributions

M.N. and C.Z. wrote the manuscript.

Corresponding author

Correspondence to Chenghang Zong.

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Competing interests

M.N. and C.Z. are cofounders and equity holders of Pioneer Genomics Inc.

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Nature Biotechnology thanks the anonymous reviewers for their contribution to the peer review of this work.

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Niu, M., Zong, C. Reply to: Methodological concerns and lack of evidence for single-synapse RNA-seq. Nat Biotechnol 41, 1225–1228 (2023). https://doi.org/10.1038/s41587-023-01878-6

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