Bioengineered uterine tissue could provide a treatment option for women with uterine factor infertility. In large animal models, reconstruction of the uterus has been demonstrated only with xenogeneic tissue grafts. Here we use biodegradable polymer scaffolds seeded with autologous cells to restore uterine structure and function in rabbits. Rabbits underwent a subtotal uterine excision and were reconstructed with autologous cell-seeded constructs, with nonseeded scaffolds or by suturing. At 6 months postimplantation, only the cell-seeded engineered uteri developed native tissue-like structures, including organized luminal/glandular epithelium, stroma, vascularized mucosa and two-layered myometrium. Only rabbits with cell-seeded constructs had normal pregnancies (four in ten) in the reconstructed segment of the uterus and supported fetal development to term and live birth. With further development, this approach may provide a regenerative medicine solution to uterine factor infertility.
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The data that supports the findings of this study are available from the corresponding author, upon reasonable request.
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This work was supported by grants from the National Institutes of Health (no. T32-EB014836-05) and the State of North Carolina. We thank J. Kassis for editorial assistance, T. Wang, H.G. Huddlestone and C. Bishop for their initial background contribution to the project and S. Lankford, A.S. Dean, I. Vasutin, T.J. Cockerham, M. Seeds and E.E. Whitaker for technical assistance.
Boston Children’s Hospital was assigned the rights to two issued patents, both titled ‘Tissue Engineered Uterus’, no. 7,049,057, filed 15 November 2002, and no. 7,429,490, filed 7 February 2005, with A.A. and J.J.Y. listed as inventors. Based on the remaining patent term and the need for further studies before this technology is used clinically, there are no current or expected financial interests related to these patents.
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Magalhaes, R.S., Williams, J.K., Yoo, K.W. et al. A tissue-engineered uterus supports live births in rabbits. Nat Biotechnol (2020). https://doi.org/10.1038/s41587-020-0547-7