Structural variation (SV), involving deletions, duplications, inversions and translocations of DNA segments, is a major source of genetic variability in somatic cells and can dysregulate cancer-related pathways. However, discovering somatic SVs in single cells has been challenging, with copy-number-neutral and complex variants typically escaping detection. Here we describe single-cell tri-channel processing (scTRIP), a computational framework that integrates read depth, template strand and haplotype phase to comprehensively discover SVs in individual cells. We surveyed SV landscapes of 565 single cells, including transformed epithelial cells and patient-derived leukemic samples, to discover abundant SV classes, including inversions, translocations and complex DNA rearrangements. Analysis of the leukemic samples revealed four times more somatic SVs than cytogenetic karyotyping, submicroscopic copy-number alterations, oncogenic copy-neutral rearrangements and a subclonal chromothripsis event. Advancing current methods, single-cell tri-channel processing can directly measure SV mutational processes in individual cells, such as breakage–fusion–bridge cycles, facilitating studies of clonal evolution, genetic mosaicism and SV formation mechanisms, which could improve disease classification for precision medicine.
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Chromothripsis is a frequent event and underlies typical genetic changes in early T-cell precursor lymphoblastic leukemia in adults
Leukemia Open Access 16 August 2022
BMC Genomics Open Access 31 July 2021
Genome Biology Open Access 30 June 2021
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Sequencing data from this study can be retrieved from the European Genome-phenome Archive (EGA) and the European Nucleotide Archive (accession codes: PRJEB30027, PRJEB30059, PRJEB8037, PRJEB33731, EGAS00001003248, EGAS00001003365). Access to human patient data is governed by the EGA Data Access Committee.
The computational code of our analytical framework is hosted on GitHub (see https://github.com/friendsofstrandseq/mosaicatcher-pipeline, https://github.com/friendsofstrandseq/TranslocatoR and https://github.com/friendsofstrandseq/mosaicatcher). All code is available freely for academic research.
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We thank W. Huber, O. Stegle, F. Marass and P. Lansdorp for discussions and T. Christiansen for software documentation. We thank M. Paulsen (Flow Cytometry Core Facility) for assistance in sorting and C. Eckert for primary T-ALL samples for engraftment and N. Habermann for project support. J.O.K. acknowledges funding from European Research Council Starting (grant no. 336045) and Consolidator (grant no. 773026) grants and the National Institutes of Health (grant no. 3U41HG007497-04S1). Funding also came from the German Research Foundation (grant nos. 391137747 and 395192176) to T.M., the José Carreras Foundation (grant no. DJCLS 06R/2016) to J.O.K., A.E.K. and J.B.K., the Baden-Württemberg Stiftung (grant no. ID16) to A.E.K. and the Iten-Kohaut Stiftung to J.P.B. A.D.S. and H.Y. received postdoctoral fellowships through the Alexander von Humboldt Foundation.
The following authors have disclosed a patent application (no. EP19169090): A.D.S., J.O.K., T.M., D.P., S.M. and M.G.
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Supplementary Figs. 1–24, Tables 1,2,6,8 and 9 and Notes
Overview of Strand-seq libraries included in the study. Metrics of the single cell sequencing data for RPE-1, C7, BM510, P33 and P1 samples, with total number of high-quality mapped fragments per library listed.
SV calls generated with our framework and using external methodologies. Overview of the single cell SV calls generated for RPE-1, C7, BM510, P33 and P1 samples, with variant allele frequencies and orthogonal validation notes included.
Presumed clonal CNA events in RPE cells detected by genomic sequencing. Data are shown for RPE-1, C7 and BM510. WGS, whole-genome sequencing; MP, mate-pair sequencing. Only regions of 200 kb and longer are reported.
Copy-number of BFB segments in single C7 cells. Description of the 10p BFB locus for every C7 cell with the CN estimate provided for each stepwise segment used to predict the BFB cycle number for that cell.
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Sanders, A.D., Meiers, S., Ghareghani, M. et al. Single-cell analysis of structural variations and complex rearrangements with tri-channel processing. Nat Biotechnol 38, 343–354 (2020). https://doi.org/10.1038/s41587-019-0366-x
Nature Reviews Genetics (2022)
Chromothripsis is a frequent event and underlies typical genetic changes in early T-cell precursor lymphoblastic leukemia in adults
Genome Biology (2021)
BMC Genomics (2021)
Nature Genetics (2021)