Fluorescent indicators can provide quantitative insights into the spatiotemporal dynamics of signalling molecules released by brain circuits. However, a mismatch between the experimental context and the experimental imaging settings often introduces unexpected errors and biases in such measurements. Appreciating this mismatch should help researchers to arrive at unbiased estimates.
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Rusakov, D.A. Avoiding bias in fluorescence sensor readout. Nat. Rev. Neurosci. 25, 1–2 (2024). https://doi.org/10.1038/s41583-023-00768-9
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DOI: https://doi.org/10.1038/s41583-023-00768-9
