Adipocyte insulin resistance is a driving force for systemic insulin resistance. Advances in human stem cell culture have established conditions for human adipocytes that give rise to a reproducible population of adipocytes that retain a high level of insulin sensitivity, paving the way for high throughput screening approaches.
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Wilding, J. P. H. et al. Once-weekly semaglutide in adults with overweight or obesity. N. Engl. J. Med. 384, 989–1002 (2021).
Jastreboff, A. M. et al. Tirzepatide once weekly for the treatment of obesity. N. Engl. J. Med. 387, 205–216 (2022).
Müller, T. D., Blüher, M., Tschöp, M. H. & DiMarchi, R. D. Anti-obesity drug discovery: advances and challenges. Nat. Rev. Drug Discov. 21, 201–223 (2022).
Friesen, M. et al. Development of a physiological insulin resistance model in human stem cell-derived adipocytes. Sci. Adv. 8, eabn7298 (2022).
Ahfeldt, T. et al. Programming human pluripotent stem cells into white and brown adipocytes. Nat. Cell Biol. 14, 209–219 (2012).
Lin, Y. et al. The hyperglycemia-induced inflammatory response in adipocytes: the role of reactive oxygen species. J. Biol. Chem. 280, 4617–4626 (2005).
Brown, M. S. & Goldstein, J. L. Selective versus total insulin resistance: a pathogenic paradox. Cell Metab. 7, 95–96 (2008).
Zhao, S., Kusminski, C. M., Elmquist, J. K. & Scherer, P. E. Leptin: less is more. Diabetes 69, 823–829 (2020).
Kono, T. & Barham, F. W. The relationship between the insulin-binding capacity of fat cells and the cellular response to insulin. Studies with intact and trypsin-treated fat cells. J. Biol. Chem. 246, 6210–6216 (1971).
Straub, L. G. et al. Antioxidants protect against diabetes by improving glucose homeostasis in mouse models of inducible insulin resistance and obesity. Diabetologia 62, 2094–2105 (2019).
L.G.S. is supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) grant #444933586. P.E.S. is supported by US National Institutes of Health (NIH) grants R01-DK55758, R01-DK127274, R01-DK099110, R01-DK131537 and RC2-DK118620.
The authors declare no competing interests.
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Straub, L.G., Scherer, P.E. Insulin sensitive human adipocytes for in vitro studies. Nat Rev Endocrinol 18, 591–592 (2022). https://doi.org/10.1038/s41574-022-00727-x