It is generally accepted that epiblast cells ingress into the primitive streak by epithelial-to-mesenchymal transition (EMT) to give rise to the mesoderm; however, it is less clear how the endoderm acquires an epithelial fate. Here, we used embryonic stem cell and mouse embryo knock‐in reporter systems to combine time-resolved lineage labelling with high-resolution single-cell transcriptomics. This allowed us to resolve the morphogenetic programs that segregate the mesoderm from the endoderm germ layer. Strikingly, while the mesoderm is formed by classical EMT, the endoderm is formed independent of the key EMT transcription factor Snail1 by mechanisms of epithelial cell plasticity. Importantly, forkhead box transcription factor A2 (Foxa2) acts as an epithelial gatekeeper and EMT suppressor to shield the endoderm from undergoing a mesenchymal transition. Altogether, these results not only establish the morphogenetic details of germ layer formation, but also have broader implications for stem cell differentiation and cancer metastasis.
The recent single-cell genomics revolution has generated transcriptional and epigenetic roadmaps for the formation of the three principal germ layers during gastrulation1,2,3. Epithelial-to-mesenchymal transition (EMT) and mesenchymal-to-epithelial transition (MET) are evolutionarily conserved processes that occur during development and are essential for gastrulation and embryonic morphogenesis, but if they are dysregulated in adulthood they lead to cancer metastasis4,5,6. Although gastrulation serves as the mechanistic basis to understand EMT, cancer metastasis, stem cell differentiation and congenital disease, its understanding is incomplete in mammals. During gastrulation, epiblast cells exit pluripotency and allocate to one of the three germ layers—the ectoderm, mesoderm or definitive endoderm lineage—to generate the progenitors of major organs in the body7. The T-box transcription factor Brachyury (T) and forkhead box transcription factor A2 (Foxa2) are master regulators of mesoderm and endoderm formation, respectively8,9,10,11. Morphogen gradients along the anterior–posterior axis induce posterior epiblast cells to undergo EMT and ingress into the primitive streak region (the space between the epiblast and visceral endoderm epithelial layers)7,12,13. Upon high Wnt/β-catenin, transforming growth factor-β (TGF-β) and fibroblast growth factor signalling, EMT transcription factors such as Mesp1 and 2 (refs. 14,15), Twist1 (ref. 16), Snail1 and 2 (refs. 16,17,18) and Zeb1 and 2 (refs. 19,20) are induced. Following the activation of an EMT program, columnar-shaped epiblast cells lose their epithelial morphology by dynamic expression changes and remodelling of apical–basal polarity and cell–cell adhesion. Simultaneously, cells adopt a mesenchymal fate by downregulation of E-cadherin and by upregulation of N-cadherin, vimentin and α-smooth muscle actin, resulting in ingression and migration of mesodermal cells into the primitive streak—classical hallmarks of EMT17,18,21,22,23. It is generally assumed that definitive endoderm (DE) progenitors undergo a further MET to give rise to the epithelial endoderm layer, in an EMT–MET cycle12,13,24,25,26,27,28; however, this idea is based on Drosophila and zebrafish model systems and has never been formally proven in mammals13. Previously, we noticed that before primitive streak formation and initiation of gastrulation, proximal T+ mesoderm and distal Foxa2+ endoderm progenitors are already segregated in the epiblast11. As Foxa2 regulates axial mesendoderm (AME) and definitive endoderm differentiation, cell polarity and epithelialization11, we hypothesized that DE progenitors leave the epiblast to form the mature definitive endoderm lineage by a process independent of a complete EMT–MET cycle.
Definitive endoderm is formed in the absence of an EMT–MET cycle
To test this hypothesis and specifically study endoderm differentiation and morphogenesis in more detail, we performed time-resolved and simultaneous lineage labelling and tracking using Foxa2–Venus fusion (FVF) and Sox17–mCherry fusion (SCF) double knock‐in reporter mouse embryonic stem cells (mESCs) and mouse embryos29,30. During gastrulation, FVFlow epiblast progenitors upregulate FVF reporter activity while they leave the epithelium and give rise to FVFhigh transitory progenitors (Extended Data Fig. 1a and Supplementary Video 1). FVFhigh transitory progenitors migrate between the epiblast and visceral endoderm layers until they upregulate SCF and intercalate into the outside visceral endoderm and give rise to the FVFhigh/SCF+ definitive endoderm lineage11,29,30. We recently noticed that it only takes ~12 h for FVF/SCF mESCs to differentiate from FVFlow progenitors into FVFhigh/SCF+ definitive endoderm30. This short timespan seems insufficient for a cell to down- and upregulate molecular machineries on the messenger RNA (mRNA) and protein level to undergo a complete EMT–MET cycle. Thus, we investigated first whether Foxa2low epiblast progenitors leave the epithelium by EMT in early-, mid- and late-streak-stage embryos. Before gastrulation, FVFlow epiblast progenitors are found in the posterior epiblast region, suggesting that epiblast cells are already fate specified (Extended Data Fig. 1b)11. During primitive streak induction and elongation, FVFlow epiblast progenitors occupy an epiblast domain distal to the morphological anterior primitive streak (APS) region (Fig. 1a,a′ and Extended Data Fig. 1c). Within the FVFlow epiblast domain, single FVFhigh transitory progenitors appear, leave the epithelium and squeeze as elongated cells between the epiblast and visceral endoderm (Fig. 1a–c, insets)11,17. This is in stark contrast with the proximal primitive streak, where T+ mesoderm cells with a mesenchymal morphology span several cell diameters across the primitive streak region (Fig. 1a–c). During gastrulation, mesoderm formation is driven by the key EMT transcription factor Snail1 (refs. 17,18). At the mid- to late-streak stage, a distinct separation of three populations in the primitive streak region was apparent (see the schematic in Fig. 1b′): a large T+ mesoderm population in the proximal primitive streak region; a few Foxa2high/T+ AME progenitors (Fig. 1b,b′); and Foxa2low epiblast progenitors in the epiblast and Foxa2high transitory progenitors that ingressed distally to the APS (Fig. 1a,b,a′,b′ and Extended Data Fig. 1a–d)11. Notably, the EMT transcription factor Snail1 was highly upregulated in T+ mesoderm progenitors, while it was weakly expressed on the mRNA level in Foxa2high transitory progenitors (Figs. 1b,b′ and Fig. 2e and Extended Data Fig. 1d) and not expressed in mature Foxa2high/Sox17+ definitive endoderm (Extended Data Fig. 1e,g,h). During the transition to a mesenchymal state, Snail1 downregulates E-cadherin17. At the same time, N-cadherin is upregulated20. Immunostaining analysis revealed that mesodermal cells within the primitive streak displayed the well-described switch from E- to N-cadherin during EMT (Fig. 1c,c′ and Extended Data Fig. 1f). In contrast, FVFhigh transitory progenitors, definitive endoderm and AME cells maintained E-cadherin and synchronously upregulated N-cadherin, as quantified by western blot and immunostaining (Fig. 1c–f and Extended Data Fig. 1f,i).
To understand the lineage bifurcations and hierarchy during gastrulation and to generate a continuous in vivo roadmap of the molecular changes, we combined FVF lineage labelling and flow sorting to perform high-throughput single-cell RNA sequencing (scRNA-seq) (Fig. 2a and Extended Data Fig. 2). This allowed us to enrich for rare transitory cell types and to map the FVFlow epiblast progenitors, FVFhigh transitory progenitors and FVFhigh AME and definitive endoderm descendants via Louvain cluster annotation (Extended Data Fig. 2a–d). Using our previously established scVelo31 and CellRank32 algorithms, we combined directional information from the RNA velocity and robustness of trajectory inference to compute fate probabilities, and identified lineage driver genes during mesoderm and endoderm segregation (Fig. 2b–d and Extended Data Fig. 2e). Combining FVF lineage labelling with pseudotime analysis revealed that, during posterior epiblast-to-endoderm transition, EMT transcription factor genes (Snail1 and 2, Zeb1 and 2, Mesp1 and 2 and Twist1) are downregulated, whereas E-cadherin (Cdh1) is maintained and N-cadherin (Cdh2) is upregulated (Fig. 2d–f). In contrast, posterior epiblast cells undergoing a mesoderm transition upregulate an EMT transcription factor program and show an E- to N-cadherin switch during mesenchymal transition. Altogether, these results suggest that, in mouse embryos, Foxa2low epiblast progenitors upregulate Foxa2 levels and give rise to Foxa2high transitory progenitors that ingress distal to the anatomical visible primitive streak to form the Foxa2high/Sox17+ definitive endoderm independent of a full EMT–MET cycle.
In vitro-generated definitive endoderm forms by partial EMT
To verify these results in vitro and simultaneously analyse mesoderm and endoderm segregation, we generated a TGFP/+; Foxa2tagRFP/+ knock-in dual-reporter mESC line (Extended Data Fig. 3a). We differentiated the mESCs in a stepwise time-resolved manner into mesoderm and endoderm (Fig. 2g and Methods) and sorted progenitors and definitive lineages by flow sorting using the reporter (TGFP and Foxa2tagRFP) and differential marker (CD24neg/low/high) expression (Extended Data Fig. 3b,c)33. Global transcriptional profiling at days 2 and 4 of differentiation revealed that mesoderm progenitors still express pluripotency markers (Pou5f1, Nanog and Sox2) and slightly upregulate mature mesoderm markers (Tbx3 and 5, Mesp1 and 2 and Hand1) (Fig. 2h). Similarly, DEPs still express pluripotency genes and already induce the expression of mature definitive endoderm markers (Sox17, Hhex, Dkk1 and Cer1) (Fig. 2h). Next, we tested whether EMT transcription factors are expressed during mesodermal and endodermal lineage acquisition in vitro. During mesoderm differentiation, EMT transcription factor genes (Zeb1 and 2, Mesp1 and 2 and Snail1) are already expressed in mesoderm progenitors and further upregulated in mesoderm, while DEP and definitive endoderm do not upregulate EMT transcription factor genes (Fig. 2h). In vitro endoderm differentiations confirmed the absence of Snail1 protein in DEPs and definitive endoderm (Extended Data Fig. 4a,b and Methods). Furthermore, E-cadherin and N-cadherin mRNA and protein were maintained or upregulated, respectively, during endoderm differentiation in vitro (Fig. 2h and Extended Data Fig. 4c,d), consistent with our in vivo results (Fig. 1c–f). Using a previously generated knock-in Foxa2–H2B–Venus (Foxa2Venus/+) mESC transcriptional reporter line34, we flow sorted Foxa2Venus low progenitor and Foxa2Venus high definitive endoderm, which maintain the epithelial marker protein E-cadherin during differentiation (Extended Data Fig. 4e–h). Re-analysis of our previously generated RNA-seq, chromatin immunoprecipitation sequencing (ChIP-seq) data from stem cell-derived endoderm34 suggested that the expression of N-cadherin as well as E-cadherin is regulated by Foxa2 (Fig. 2h and Supplementary Table 1). Taken together, nascent endoderm expresses epithelial markers from specification to determination in vitro and in vivo. Furthermore, definitive endoderm is formed independent of an E- to N-cadherin switch and substantial upregulation of well-known EMT transcription factors, suggesting it is formed by partial EMT.
The EMT transcription factor Snail1 is not required for endoderm formation
Several knockout studies have indicated redundant functions of the EMT transcription factors Zeb1 and 2 (refs. 35,36) and Snail2 (ref. 37) during gastrulation, whereas mutant embryos with alterations in Snail1 fail to downregulate E-cadherin and show defective EMT and mesoderm formation17,18. To test whether Snail1 function is required for definitive endoderm formation, we generated a Snail1 knockout mESC line (Extended Data Fig. 5a,b). After 3 d of definitive endoderm differentiation, we detected comparable numbers of definitive endoderm cells (Foxa2high/Sox17+) formed from both wild-type (control) and Snail1 knockout mESCs (Fig. 3a–d and Extended Data Fig. 5c–f). To analyse definitive endoderm formation in vivo, we generated completely mESC-derived embryos by tetraploid aggregation (Fig. 3e–g)38. This allowed us to observe nascent definitive endoderm formation (derived from mESCs) and visceral endoderm dispersal (derived from tetraploid embryos expressing membrane Tomato (mT)39) by means of fluorescent marker gene expression. At the early headfold stage, we observed that the visceral endoderm (mT+) was clearly dispersed by Snail1 knockout mESC-derived definitive endoderm (Sox17+), comparable to control chimeras (Fig. 3e–g). Together, these findings demonstrate that the master EMT transcription factor Snail1 is not necessary for definitive endoderm formation in vitro and in vivo.
Foxa2 suppresses a complete EMT during endoderm formation
To better understand how definitive endoderm is formed during gastrulation, we investigated the role of Foxa2, which has been shown to be crucially important for the formation of epithelial lineages (that is, ADE and AME)9,10. For this purpose, we used our previously established knock-in/knockout Foxa2 reporter line for endoderm differentiations and re-analysed our RNA-seq and ChIP-seq datasets34. As predicted, we observed a lack of definitive endoderm formation and mature marker expression using the homozygous Foxa2Venus/Venus compared with heterozygous Foxa2Venus/+ definitive endoderm cells (Fig. 4a,b). This resembled the well-known phenotype of Foxa2 mutant embryos9,10,11,35. Interestingly, we noted that, upon loss of Foxa2, EMT transcription factor genes (Snail1, Mesp1, Tbx3 and Zeb1 and 2) and the EMT marker gene (Vim) were upregulated in Foxa2Venus/Venus mutant cells (Fig. 4b–d). To analyse whether Snail1 is also upregulated in the Foxa2 lineage in vivo, we generated mESC-derived embryos using heterozygous Foxa2Venus/+ and homozygous Foxa2Venus/Venus knock-in/knockout mESCs by tetraploid complementation (Fig. 4e–h). At the late-streak stage, the APS region was thickened due to an accumulation of Foxa2 lineage-positive cells in the Foxa2Venus/Venus mESC-derived embryo (compare Fig. 4f,g)11. We also noticed more Venus lineage-labelled Foxa2 mutant cells with clear upregulation of the EMT transcription factor Snail1 (Fig. 4f–h). Induction of Snail1 expression in the Foxa2Venus/Venus mutant lineage-labelled cells in vitro as well as in vivo implied that Foxa2 suppresses the key EMT transcription factor Snail1 to prevent E-cadherin downregulation and mesenchymal transition.
Foxa2 serves as an epithelial gatekeeper in the endoderm
TGF-β and nodal and canonical Wnt/β-catenin signalling activates EMT transcription factors and initiates gastrulation40,41,42. Recently, it was shown in different cancer and epithelial cell lines that the EMT key regulator Snail1 is induced by the TGF-β ligand 1 (ref. 43) and Wnt/β-catenin activation by Gsk3β inhibition37,44,45. In FVFhigh transitory progenitors and definitive endoderm, we did not observe substantial upregulation of Snail1 expression and synthesis (Figs. 1 and 2); however, in homozygous Foxa2Venus/Venus mutant cells, Snail1 was highly expressed (Fig. 4c–h), suggesting that Foxa2 is a potential repressor of Snail1. However, ChIP-seq analysis of definitive endoderm cells revealed that Snail1 cis-regulatory elements are not directly bound by Foxa2 (Fig. 2h and Supplementary Table 1)34. Next, we analysed the activity of Wnt signalling genes and observed that canonical Wnt ligands and its targets, such as Wnt3 and APC, are not upregulated in definitive endoderm, in contrast with T+ mesoderm, which highly expresses these genes (Fig. 5a,b). Interestingly, secreted inhibitors of the canonical Wnt/β-catenin pathway (Cer1, Dkk1 and Srfp1 and 5) are highly upregulated in definitive endoderm but downregulated in Foxa2Venus/Venus knockout cells (Figs. 4b and 5a,b). Further analysis of the ChIP-seq data suggested that several of the secreted inhibitors of the canonical Wnt/β-catenin pathway are directly regulated by Foxa2, such as Cer1 (Fig. 5b and Supplementary Table 1). During gastrulation, we observed downregulation of Wnt ligands (Wnt3 and Wnt3a) and the target gene (Lef1), as well as upregulation of secreted Wnt inhibitors (Cer1, Dkk1 and Sfrp1 and 5) in the transition from the posterior epiblast to transitory progenitors and definitive endoderm (Fig. 5d,e). Interestingly, the AVE and ADE marker protein Cer1 is already upregulated in Foxa2high transitory progenitors and definitive endoderm at the posterior side of gastrula embryos (Figs. 5f and 6a,b), which correlates with the downregulation of the Wnt target gene and transcription factor Lef1 (Fig. 6a–d). In line with our Foxa2 knockout results from in vitro differentiations (Fig. 4b), we also observed a lack of Cer1 expression in Venus+ lineage-labelled cells of Foxa2Venus/Venus aggregation chimeras (Fig. 5g) and concomitant upregulation of the Wnt target gene Lef1 and the EMT transcription factor Snail1 (Fig. 6e–g). These results suggest that Foxa2 directly induces the expression of Wnt inhibitors and thereby indirectly inhibits Wnt/β-catenin signalling and target genes, such as Lef1 and Snail1 (Fig. 6h)37,44,45,46,47.
To test whether inhibition of canonical Wnt signalling represses Snail1 expression and promotes epithelial endoderm formation, we differentiated TGFP/+; Foxa2tagRFP/+ mESCs for 1 d with addition of the Gsk3β inhibitor CHIR and activin A to induce endoderm formation (Extended Data Fig. 6a–d). Next, we supplemented the medium with the Wnt inhibitor Dickkopf 1 (DKK1) or the Wnt ligand secretion inhibitor IWP2 for the following 2 d. The cells treated with Wnt inhibitors showed decreased Snail1 expression compared with control cells, which was expected and confirmed that Snail1 is a Wnt/β-catenin target gene37,44,45. Interestingly, the Foxa2+/Sox17+ definitive endoderm population was increased from ~11 to ~51% in DKK1-supplemented cultures and ~60% in IWP2-supplemented cultures (Extended Data Fig. 6e–g). Altogether, these results show that Foxa2 activates Wnt/β-catenin inhibitors to indirectly repress Snail1 expression to promote progenitor differentiation towards an epithelial endodermal fate in vitro and in vivo.
During endoderm formation, Foxa2 regulates a molecular program to induce and maintain endodermal polarization and epithelialization11,48. Consistently, we observed that FVFhigh transitory progenitors and FVFhigh/Sox17+ definitive endoderm descendants do not undergo a complete EMT–MET cycle, but instead acquire epithelial cell plasticity to change their morphology from a columnar- to a squamous-shaped epithelium (Figs. 1 and 2, Extended Data Fig. 1a and Supplementary Video 1)11,29,30. Interestingly, we found upregulation of several EMT suppressors, such as GRHL2 (refs. 49,50), Ovol2 (ref. 51), Pofut2 (ref. 52) and Elf3 (ref. 53) in endoderm compared with mesoderm (Fig. 5c). Furthermore, ChIP-seq data analysis suggested that Ovol2 is bound and potentially regulated by Foxa2 (Fig. 5c and Supplementary Table 1). Analysis of RNA-seq data from Foxa2Venus/+ and Foxa2Venus/Venus mESC differentiations revealed that these EMT inhibitors are downregulated upon loss of Foxa2 (Fig. 4b). Altogether, these findings show that Foxa2 not only counteracts a complete EMT in endoderm progenitors and the definitive endoderm lineage by the induction of Wnt inhibitors and EMT suppressors, but also quickly re-establishes an epithelial identity by the activation of target genes that regulate cell polarity and adhesion11. Thus, Foxa2 serves as an epithelial gatekeeper and EMT suppressor during endoderm formation.
Dynamic molecular changes drive endoderm differentiation
Finally, to understand how the definitive endoderm is formed on the morphogenetic level, we analysed and verified our time-resolved and lineage-mapped scRNA-seq data. We first compared differences of the anterior versus posterior epiblast epithelium. This disclosed lower expression of apical–basal polarity (Crb3, Ezr and EBP50) and tight junction genes (Cldn6 and 7) (Extended Data Fig. 7a,b). Both adherens junction E-cadherin and the tight junction protein Claudin7 were less abundant in posterior epiblast cells, and Claudin7 appeared in the cytoplasm and less at the plasma membrane (Fig. 7a,b and Extended Data Fig. 7c). Next, we compared Foxa2− with Foxa2+ posterior epiblast cells (Extended Data Fig. 7a,d). Foxa2+ posterior epiblast cells showed a distinct transcription factor gene expression profile (Foxa2, Eomes, Mixl1, Lhx1 and Gsc), but also particularly high expression of metalloproteinase genes (Adamts9, Adam19 and Mmp14) involved in basement membrane remodelling and morphogenetic dynamics during gastrulation54. Interestingly, the high expression of metalloproteinases was maintained in Foxa2high transitory progenitors (Fig. 7) and correlated with basement membrane remodelling in Foxa2+ posterior epiblasts (Fig. 7 and Extended Data Fig. 7e–g). Interestingly, we observed a transient downregulation of apical–basal polarity (Ezr, Crb3, EBP50, Pard6b and Scribble) and tight junction genes (Cldn6 and 7) and proteins in the endoderm transition from Foxa2low posterior epiblast to Foxa2high transitory progenitors and definitive endoderm (Fig. 7 and Extended Data Fig. 7h), probably due to a lack of basement membrane polarity cues. Altogether, the transition of columnar Foxa2low posterior epiblast through Foxa2high transitory progenitors to squamous Foxa2high/Sox17+ definitive endoderm shows several attributes of an epithelial cell plasticity program, including simultaneous expression of E- and N-cadherin and transient downregulation of polarity and adherens junction and tight junction proteins, best defined by a partial EMT20,55.
Here, we present a revised concept of germ layer formation during gastrulation (Fig. 7h). Before gastrulation is initiated, T+ mesoderm progenitors and Foxa2low epiblast progenitors are already specified in the posterior epiblast. Mesoderm progenitors ingress into the primitive streak by a complete EMT to commit to a mesenchymal fate. In contrast, Foxa2+ posterior epiblasts and Foxa2high transitory progenitors leave the posterior epiblast epithelium by transient upregulation of metalloproteinases, remodelling of the basement membrane and a transient downregulation of apical–basal polarity genes and proteins—features reminiscent of a partial EMT program. During this process, we observed an absence of EMT transcription factors, maintained E-cadherin expression, activation of EMT suppressors and expression of adherens junction and tight junction genes and proteins12,13,24,25,26,27,28. The co-expression of E- and N-cadherin in the nascent definitive endoderm and visceral endoderm probably increases the segregation of the endoderm from the mesoderm germ layer by differential cell adhesion, as proposed by Townes and Holtfreter in 195556. Previously, we have shown that Foxa2high transitory progenitors re-establish apical–basal polarity, adherens junctions and tight junctions while they start to express Sox17 and intercalate into the overlying visceral endoderm layer11,29,30.
Importantly, Foxa2 acts as an epithelial gatekeeper and EMT suppressor to protect endoderm progenitors from undergoing a complete mesenchymal transition. Foxa2 is necessary to prevent EMT transcription factor activation via the activation of canonical Wnt/β-catenin signalling inhibitors. Thus, these results are not only important to understand basic mechanisms of gastrulation, but also have broader implications, as EMT causes detrimental cancer metastasis. Importantly, endoderm-derived lung, pancreatic and colorectal cancers are among the most common and deadliest cancers worldwide (National Cancer Institute). So far, cancer metastasis was always associated with EMT but recent studies contradict the necessity of a complete EMT–MET cycle for the dissemination of cancer cells and for invasion and metastasis in pancreatic cancer55,57,58. Mechanisms of epithelial cell plasticity might allow cancer cell dissemination and metastasis and require further in-depth mechanistic studies to provide alternative targets for therapeutic intervention. In line with our basic developmental biology findings, it was shown that Foxa2 antagonizes a full EMT process in pancreatic and lung cancer59,60,61. Foxa2 is an EMT suppressor but also an epithelial gatekeeper; thus, maintaining FOXA2 expression during cancer initiation and progression will prevent a complete EMT–MET cycle and eventually cancer dissemination.
FVF30, SCF × FVF29, mT/mG39 and CD-1 mice were kept and experiments were performed at the central facilities at the Helmholtz Zentrum München German Research Center for Environmental Health in accordance with German animal welfare legislation and acknowledged guidelines of the Society for Laboratory Animal Science and Federation of European Laboratory Animal Science Associations. Mice were kept under specific pathogen-free conditions in animal rooms with a light/dark cycle of 12 h/12 h, a temperature of 20–24 °C and a humidity of 45–65%. Mice received sterile filtered water and a standard diet for rodents ad libitum. For embryo generation, females at the age of ≥6 weeks and males at the age of ≥8 weeks were used.
The cell lines used during this study were as follows: TGFP/+;Foxa2tagRFP/+ (ref. 62) G4 mESCs, FVF IDG3.2 mESCs30, IDG3.2 mESCs63, Snail1 knockout IDG3.2 mESCs and Foxa2–H2B–Venus IDG3.2 mESCs34.
The strategies for targeting TGFP/+; Foxa2tagRFP/+ and Snail1 knockout constructs are outlined in Extended Data Figs. 3a and 5. To generate the TGFP/+; Foxa2tagRFP/+ dual-reporter mESC line, we generated a targeting vector where the open reading frame (ORF) of Foxa2 in exon 3 was fused to the ORF of the red fluorescent protein (RFP) tagRFP, followed by a phospho-glycerate kinase promoter-driven Neomycin (Neo) resistance gene cassette flanked by two loxP sites. The plasmid construct was introduced by electroporation into an available T-GFP knock-in mESCs line, in which the Brachyury expression was disrupted by insertion of a green fluorescent protein (GFP) mini gene62. Neo-resistant clones were analysed by Southern blot and PCR with the primers EP064, EP397, EP398 and EP1320 (Supplementary Table 2).
To generate Snail1 knockout mESCs, we designed a targeting vector that replaced the Snail1 ORF by an H2B–Venus–Intron–polyA Neo cassette64. Two guide RNAs (gRNAs) were designed to cut around the start codon of Snail1 (gRNAs 101 and 129) and a further two gRNAs, used to cut around the stop codon of Snail1 (gRNAs 4 and 7), were cloned into the pbs–U6 vector. mESCs were transfected with the Snail1 knockout targeting vector, four gRNAs and Cas9–D10A overexpression vector (pCAG–Cas9–D10A–bpA). At 48 h after transfection, cells were selected with G418 and picked clones were analysed by PCR genotyping (Extended Data Fig. 5 and Supplementary Table 2). The recombination borders of the targeting vector and wild-type sequence were analysed by sequencing of the knock-in-specific PCR product.
Aggregation chimeras were generated as described by Artus and Hadjantonakis38.
Immunofluorescence stainings of whole-mount embryos
Embryos (E6.5–7.5) were dissected in Dulbecco’s phosphate-buffered saline (DPBS) and immediately fixed for 20 min with 2% paraformaldehyde (PFA) in DPBS at room temperature while shaking. The fixation was stopped by rinsing the embryos 2× with DPBS containing 0.1% Tween 20 (Merck; P9416) (DPBST). The embryos were permeabilized for 10 min (≤E7.5) to 15 min (>E7.5) using 0.1 M glycine (Merck; G8898) and 0.1% Triton X-100 (Merck; 108643) in Milli-Q water, then rinsed 2× with DPBST. Unspecific antibody binding was prevented by incubating the embryos in blocking solution containing 0.1% Tween 20, 10% heat-inactivated foetal calf serum (FCS), 0.1% bovine serum albumin (BSA) and 3% donkey serum in DPBST for ≥1 h at room temperature while shaking. Subsequently, the primary antibodies diluted in blocking solution (for dilutions, see the antibody list in Supplementary Table 3) were added and the embryos were kept at 4 °C overnight on a shaker and for another 1–2 h at room temperature the following day. The embryos were rinsed 2× and washed 3× for 10 min with DPBST. Subsequently, the embryos were exposed to the secondary antibodies diluted in blocking solution for at least 3 h at room temperature on a shaker. The secondary antibodies were replaced by a 4′,6-diamidino-2-phenylindole (DAPI)/DPBST solution (2 µg ml−1 DAPI in DPBST) and incubated for 20 min at room temperature. Afterwards, the embryos were rinsed twice and then washed 3× for 10 min with DPBST. The embryos were dehydrated in 15 and 30% glycerol in DPBS, each for 10 min at room temperature. The embryos were then embedded in antifade between two cover slips using a 100-µm spacer, dried at room temperature and stored at 4 °C until imaging. A list of primary and secondary antibodies is shown in Supplementary Table 3.
Embryos were fixed in 4% PFA overnight, embedded in HistoGel (Richard-Allan Scientific; HG-4000-144), dehydrated in alcohol gradients, embedded in wax blocks and sectioned at 6-µm thickness. Slides were cleared with xylene, rehydrated and permeabilized in 1% sodium dodecyl sulfate (SDS) before performing antigen retrieval (Diva Decloaker; Biocare Medical).
Blocking was done with 10% normal donkey serum and incubation was performed with primary antibodies overnight at 4 °C. Slides were then washed 3× with PBST and incubated with fluorophore-conjugated secondary antibodies for 1 h at room temperature in the dark. Nuclei were stained with DAPI (Life Technologies, 1:500–1:1,000). After washing, the slides were finally mounted with Vectashield (Vector Laboratories; H-1000-10) and imaged using a confocal microscope.
Immunofluorescence stainings of cryosections from embryos
Embryos were fixed for 30 min to 1 h in 2% PFA, washed twice in PBS and dehydrated in 5, 10, 15, 20 and 30% sucrose (1 h each) and 30% sucrose/O.C.T. (Tissue-Tek; 4583) (1:1) overnight at 4 °C. Embryos were sectioned at 12- to 15-µm thickness and stored at −20 °C until usage. The sectioned embryos were permeabilized and blocked (see above). Primary and secondary antibody staining was performed as described above. Finally, the slides were mounted with Vectashield and kept for 24 h at room temperature to dry. Supplementary Table 3 lists the antibodies and dilutions used.
Immunofluorescence stainings of cells
Differentiated cells were washed with PBS and fixed for 15 min with 4% PFA. The cells were immunostained as described in the previous section. The cells were kept in PBS for immediate imaging. Detailed information about the primary and secondary antibodies used is provided in Supplementary Table 3.
Immunofluorescence stainings for flow cytometry analysis
Differentiated mESCs were dissociated using Accutase (Merck; A6964) or 0.05% trypsin (Life Technologies; 25300054) and fixed in 4% PFA for 10 min. The cells were permeabilized for 15 min and blocked for 1 h at room temperature (see above). Next, primary antibodies were diluted in blocking solution and incubated for 3–4 h at room temperature or overnight at 4 °C. Cells were washed 3× with PBS for 10 min each and secondary antibody solution was added for 1–2 h at room temperature. After another washing of 3× with PBS, samples were analysed by BD FACSAria III and FlowJo version 10.2. The gates were determined by secondary antibody controls. A list of all primary, secondary and conjugated antibodies is provided in Supplementary Table 3.
Fluorescence-activated cell sorting (FACS) for scRNA-seq and western blotting
Embryos were isolated and extraembryonic compartments were removed mechanically. Embryonic compartments were washed with cold PBS. For single-cell suspension, FVF embryos and differentiated Foxa2Venus/+ mESCs were incubated with TrypLE (Life Technologies; 12605) for 10 min at 37 °C. Cells were dissociated by pipetting up and down. To exclude dead cells, samples were incubated for 5–10 min with either 7-aminoactinomycin D or DAPI on ice in the dark. The samples were then washed twice, resuspended in FACS buffer (PBS, 2% FCS and 2 mM ethylenediaminetetraacetic acid) and loaded for flow sorting. The gating strategy was as follows: main population > single cells > living cells (7-aminoactinomycin D/DAPI negative) > FVFneg, FVFlow and FVFhigh cells. For scRNA-seq analysis, the cells were collected in RNA-seq buffer (PBS and 1% FCS). For western blotting analysis, the cells were collected in PBS and spun down and the pellet was snap frozen until it was used.
For western blotting of FACS-sorted cells, the cell pellet was dissociated by RIPA buffer (75 mM NaCl, 6.37 mM sodium deoxycholate, 0.005% NP-40, 0.05% SDS and 25 mM Tris (pH 8)) supplemented with phosphatase inhibitor cocktail. The cell lysates were resolved by SDS–polyacrylamide gel electrophoresis, then transferred to a polyvinylidene fluoride membrane (Bio-Rad) and blocked in 5% BSA–TBST (TBS + 0.1% Tween 20) for 1 h. The primary antibodies were incubated in 5% BSA–TBST overnight at 4 °C on a shaker. The following day, the membranes were washed at least three times for 15 min in TBST on a shaker. The secondary horseradish peroxidase-conjugated antibodies were incubated with shaking for 1–2 h at room temperature. After washing the membranes with TBST three times for 15 min each, the bands were visualized by adding Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific). The bands were quantified with ImageJ version 1.53c. Supplementary Table 3 lists the primary and secondary antibodies and their dilutions.
Images from immunostained embryos were acquired with Leica SP5 and Zeiss LSM 880 Airyscan confocal microscopes. Images taken with the Leica confocal microscope were analysed using Leica LAS AF Lite 4.0 and images taken with the Zeiss confocal microscope were processed using Zeiss Zen 2.3 lite Blue software.
Western blots were quantified with ImageJ version 1.53c by determining the pixel density of the protein of interest. After background subtraction, the ratio was determined by protein of interest/loading control.
For all of the quantifications of different cell populations, an entire confocal z stack of an embryo was analysed using ImageJ version 1.53c. For every staining, three individual embryos were processed in the same way. In brief, for Fig. 3h, Sox17+mT− (definitive endoderm) cells and Sox17+mT+ (visceral endoderm) cells at the surface of the Snail1 variant and wild-type embryos were counted. For Fig. 4h, Venushigh and either Snail1+ or Snail1− cells were counted throughout the heterozygous and homozygous Foxa2 variant embryos. For Fig. 6g, Venushigh or Foxa2high cells co-expressing Snail1 and/or Lef1low/high were counted throughout the entire wild-type or Foxa2 variant embryos, excluding the visceral endoderm/definitive endoderm at the surface of the embryo. For Extended Data Fig. 1g, Foxa2, T and Snail1low/mid/high-expressing cells in mesoderm, AME and Foxa2high transitory progenitors (based on anatomical position and marker expression) were analysed throughout a complete wild-type embryo, excluding the visceral endoderm/definitive endoderm at the surface of the embryo. For Extended Data Fig. 1i, FVF+ epiblast progenitors, FVF, Foxa2high transitory progenitors and Foxa2high visceral endoderm/definitive endoderm (based on anatomical location and marker expression) were quantified for E-cadherin and N-cadherin expression in FVF embryos. In Extended Data Fig. 1h, Foxa2high transitory progenitors, Foxa2high transitory progenitors/visceral endoderm/definitive endoderm and AME (based on anatomical position and marker expression) were analysed for Foxa2, Sox17 and Snail1 expression in wild-type embryos. In Extended Data Fig. 7c, the intensity of E-cadherin was measured in a rectangular area at three different positions within the anterior or posterior epiblast from one z plane of FVF embryos. The intensity of Claudin7 was measured in a rectangular area at three different positions within the anterior or posterior epiblast of wild-type embryos.
Cell culture and differentiation
Mouse ESCs were cultured on mitomycin C-treated mouse embryonic fibroblasts (feeders) in Dulbecco’s modified Eagle’s medium (Life Technologies; 11965092) supplemented with 15% FCS (PAN-Biotech; P30-2602), 0.1 mM β-mercaptoethanol (Life Technologies; 31350-10), 2 mM l-glutamine (Life Technologies; 25030081), 1× non-essential amino acids (Sigma–Aldrich; M7145), 2 mM HEPES (Life Technologies; 15630-056) and 1,000 U ml−1 leukaemia inhibitory factor (Sigma–Aldrich; ESG1107). Every 2–3 d, cells were passaged by treatment with 0.05% trypsin (Life Technologies; 25300054) on new feeders and the medium was changed every day. For differentiations of the Snail1 knockout mESCs, TGFP/+; Foxa2tagRFP/+ mESCs and IDG3.2 mESCs, 1 × 105 cells per 1 cm2 were seeded in chemically defined medium, as published recently65. After 24 h, endoderm was induced by the addition of 2.5 µM CHIR99021 (Miltenyi Biotec; 130-103-926) for 1 d and 25 ng ml−1 activin A (Peprotech; 120-14-300) for all 3 d. For the inhibitor experiments, endoderm was induced as described before, and on days 2 and 3 either 400 ng ml−1 DKK1 (Peprotech; 120-30-50) or 1.25 µM IWP2 (Tocris; 3533-10) was supplemented. Differentiations of the FVF mESCs and Foxa2Venus/Venus knockout mESCs were performed as described before34. The differentiation of TGFP/+; Foxa2tagRFP/+ mESCs followed by microarray analysis was carried out based on a previously published protocol34. Briefly, before differentiation, Wnt3a feeders were seeded at a density of ~5 × 104 cells per 24 wells. ESCs were passaged onto gelatine-coated plates for 30 min twice to remove remaining feeders from the maintenance cultures. Next, ESCs were transferred to a Wnt3a-expressing feeder plate with a seeding density of 2 × 105 cells per 24 wells. Endoderm differentiation medium, consisting of 500 ml advanced Dulbecco’s modified Eagle’s medium/F-12 (Life Technologies; 12634-10), 500 ml advanced RPMI 1640 (Life Technologies; 12633-012), 22 ml GlutaMAX (Life Technologies; 12860-01), 200 µl AlbuMAX 100 mg ml−1 (Life Technologies; 11021-029), 22 ml HEPES 1 M, 70 µl cytidine 150 mg ml−1 (Sigma–Aldrich; C4654), 0.9 ml β-mercaptoethanol 50 mM, 12 ml penicillin/streptomycin 10,000 U ml−1 (Life Technologies; 10378016) and 1 ml Insulin-Transferrin-Selenium-Ethanolamine (Life Technologies; 51500-056) supplemented with 12 ng ml−1 activin A, was used to induce endoderm differentiation. For mesoderm differentiation, 3 ng ml−1 activin A and 7.5 ng ml−1 Bmp4 were added to the endoderm differentiation medium. The medium was changed every day.
Affymetrix microarray analysis
RNA was extracted using an miRNeasy Mini Kit (Qiagen; 217004) and total RNA (150 ng) amplified using the Ambion WT Expression Kit and the WT Terminal Labeling Kit (Affymetrix). Amplified complementary DNA was hybridized on Affymetrix Mouse Gene ST 1.0 arrays. Staining and scanning (Fluidics Script FS450_0007) were done according to the Affymetrix expression protocol. An expression console (version 1.2; Affymetrix) was used for quality control. CEL files were imported into R (3.6.3) and robust multichip average normalized using the oligo (1.48.0) package. Data were then pre-filtered and 32,000 probesets with the highest mean expression across all samples were kept for further analysis. Genewise testing for differential expression was performed using limma (3.40.6) and genes with an absolute log2[fold change] of >1 and an adjusted P < 0.05 were defined as differentially expressed. All microarray data are available from the (NCBI) Gene Expression Omnibus (GEO) under accession GSE148226.
RNA-seq data analysis
RNA-seq data from Foxa2 knockout definitive endoderm (processed *rsem.genes.results.txt.gz files) were downloaded from the National Center for Biotechnology Information (NCBI) GEO (GSE116262) and analysed in R (3.6.3). RSEM output files were imported into R using the tximport (1.12.3) package and batch correction was performed using the RUVr function (k = 1) from the RUVseq (1.18.0) package. Counts were then prepared for DESeq2 (1.20.0) and genes with a minimum mean count below 5 were filtered out. DESeq2 was run with default parameters and log[fold change] shrinkage was performed using apeglm (1.6.0) with the options svalue = TRUE, lfcThreshold = log2[1.5]. Differentially expressed genes were defined by an s value66 of <0.05.
ChIP-seq data analysis
Foxa2 ChIP-seq raw data (FASTQ files) were downloaded from the NCBI GEO (GSE116262). Trimmomatic (0.39) was used to trim low-quality bases and adapter contaminations, and Bowtie 2 (220.127.116.11) with the --very-sensitive option was used to map reads to the mm10 genome. Peaks were called using GEM (3.4), with the options --k_min 10 --k_max 13, and filtered using q value cut-offs of 10−4. Binding sites were then mapped to putative target genes by assigning peaks within 20 kilobases of a transcription start site or within a gene body to the respective gene. Genes with Foxa2 binding sites that were regulated in the Foxa2 knockout RNA-seq data were considered to be regulated by Foxa2. The list of genes is provided in Supplementary Table 1.
Single-cell libraries were generated using the Chromium Single Cell 3′ library and Gel Bead Kit version 2 (10X Genomics; 120237) according to the manufacturer’s instructions. Libraries were sequenced on the HiSeq 4000 (Illumina) with 150-base pair paired-end sequencing of read 2.
Computational analysis of scRNA-seq data
Pre-processing of droplet-based scRNA-seq data
Demultiplexing and alignment to the mm10 mouse genome, identification of unique molecular identifiers (UMIs) and barcode filtering were performed using the Cell Ranger toolkit (version 2.0.0) provided by 10X Genomics. In addition, the velocyto pipeline67, which generated a loompy file, was used to determine unspliced and spliced genes. We considered only cells with at least 1,000 expressed genes, where a gene was counted as expressed if we found at least one UMI mapped to it. We further filtered cells with a total UMI count of >125,000 or a fraction of counts from mitochondrial genes of >8%, indicative of stressed or dying cells. We did not apply a minimum library size filter because almost all cells had a total UMI count above 5,000. Cells from all samples were divided by library size, with a target sum of 50,000 UMIs (counts per million normalization; pp.normalize_per_cell in scanpy version 18.104.22.168.dev6+gfa408dc7 in Python 3.7)68, and subsequently log + 1 scaled. Then, 3,000 highly variable genes were computed as follows: genes were binned in 20 groups by mean expression and a normalized dispersion was obtained by scaling with the mean and s.d. of the dispersions for genes falling into a given bin for mean expression of genes (pp.highly_variable_genes in scanpy version 22.214.171.124.dev6+gfa408dc7 in Python 3.7 with the flavour cell_ranger to compute normalized dispersions), thereby accounting for differences across batches. Batch-effect correction was done with Scanorama version 1.4 (ref. 69) using the corrected feature space. Spliced and unspliced UMI matrices were filtered with a minimum of 20 UMIs each. Then, the data were counts per million normalized and log + 1 scaled. Furthermore, the dataset was restricted to the same highly variable genes as the count matrix.
We performed our analyses with scanpy version 126.96.36.199.dev6+gfa408dc7 in Python 3.7. First, we computed a principal component analysis (PCA) space with n = 50 components and a k-nearest neighbour graph on the PCA space with k = 30 (tl.pca and pp.neighbors). PCA and UMAP embeddings was computed on the batch-corrected and normalized data with 3,000 highly variable genes. We then used UMAP70 to represent the data in the two-dimensional embedding (tl.umap).
Clustering and cell-type annotation
The data matrix was clustered with the Louvain algorithm (tl.louvain with resolution 2.0)71 and found 27 clusters. All clusters were then inspected for potential substructure and five clusters were further resolved with a low resolution of 0.2. Here, we annotated and merged clusters again according to marker gene expression, resulting in 11 cell stages. Marker gene expression was visualized in a dotplot using scanpy version 1.6.0. Furthermore, we classified the cells according to the cell sorting into FVFneg, FVFlow and FVFhigh cells, respectively, determined the amount of Foxa2 mRNA in each cell and classified Foxa2 mRNA-negative and -positive cells based on non-batch-corrected values, where every cell counted as positive with at least one UMI of the Foxa2 gene detected. In FVFneg samples, the number of Foxa2 mRNA-positive cells was 45 out of 4,173 cells and we considered both categories as FVFneg.
Differential expression analysis
To study differential expression, we used the default scanpy test function (tl.rank_genes_groups with default method t-test_overestim_var and Benjamini–Hochberg correction for multiple testing) to determine the pairwise differences of several populations (that is, epiblast and posterior epiblast, Foxa2 mRNA+ and Foxa2 mRNA− posterior epiblast, posterior epiblast and transitory progenitors). We then visualized the top 100 up- and downregulated differentially expressed genes, respectively.
Identifying cell differentiation trajectories
To derive cell trajectories, we computed a pseudotemporal ordering using diffusion pseudotime (tl.dpt in scanpy) as a basis for subsequent visualization of gene trajectories towards the endoderm and mesoderm lineage. Next, we used the dynamical model of the scvelo package version 0.2.1 (ref. 31) in scanpy version 1.5.1 to determine gene dynamics and potential cell fate decisions towards the endoderm and mesoderm lineage (tl.recover_dynamics and tl.velocity from the scVelo package). To further characterize the cell fate decision process and to leverage the sorting information of the FVF reporter, we used the CellRank package version 1.0.0-rc.0 (ref. 32). Specifically, we recomputed the dynamical RNA velocity model excluding extraembryonic tissues and used it in CellRank, which creates a vector field from the data and uses a random walk model to determine initial, intermediate and terminal states in the data (metastable states). We identified six metastable states in the epiblasts (initial), posterior epiblast (intermediate), AME, definitive endoderm, lateral plate mesoderm and nascent endothelium (all terminal). Using the random walk model, we determined a fate probability (that is, the probability of each cell ending up in one of the metastable states). The fate probability of each cell was displayed in a uniform manifold approximation and projection (UMAP) plot, where the colour of the cell was determined by the cluster with the highest fate probability.
To determine the topology of the data and cluster-wise relationships of the cells, we used partition-based graph abstraction (PAGA)72 to quantify the connections between the clusters (tl.paga in scanpy version 1.5.1). We display all connections with a scaled connectivity of at least 0.05 (threshold parameter in pl.paga in scanpy) and visualized the fate probability of each cell population as a pie chart (pl.cluster_fates with the mode paga-pie in CellRank). We further determined the differences in fate probability in the posterior epiblast FVF-sorted Foxa2 mRNA-classified cells and displayed the fate probability per group as a bar chart (pl.cluster_fates with the mode bar in CellRank).
We further used CellRank’s compute_lineage_drivers function to determine lineage drivers for each metastable state as the correlation of the gene expression with the respective lineage. Then, we highlighted the correlation with lateral plate mesoderm and definitive endoderm in a scatter plot.
Statistics and reproducibility
In general, all of the experiments were performed, if possible, with at least three independent biological samples. Sample sizes are provided in the figure captions. Fewer than three independent experiments were used for the FVF embryo sorting and western blot analysis due to the high quantity of embryos required for this experiment. However, a total number of 158 embryos in two independent experiments was used and considered as sufficient. No statistical method was used to predetermine sample size. Data were excluded when immunohistochemical stainings were insufficient, mESC differentiations failed (<2% Foxa2+ cells), embryos were ruptured or embryos were at the wrong stage. For the scRNA-seq analysis, one of the FACS sorted FVFneg samples (FVF_neg_3) was excluded from the analysis due to low sequencing depth. The experiments were randomized if possible. The investigators were not blinded to allocation during the experiments and outcome assessment. All replications were successful. The data were analysed using GraphPad Prism software 8 (GraphPad Software).
Further information on experimental design is available in the Life Sciences Reporting Summary linked to this article.
The sequencing data that support the findings of this study have been deposited in the Gene Expression Omnibus (GEO) under the accession codes GSE148226 and GSE162534. Previously published sequencing data that were re-analysed here are available under accession codes GSE116262 (samples GSM3223321, GSM3223325, GSM3223326, GSM3223342–GSM3223345, SM3597790 and GSM3597791) Source data are provided with this paper. All other data supporting the findings of this study are available from the corresponding author upon reasonable request.
The analyses of scRNA-seq data are available at https://github.com/theislab/gastrulation_analysis.
Argelaguet, R. et al. Multi-omics profiling of mouse gastrulation at single-cell resolution. Nature 576, 487–491 (2019).
Pijuan-Sala, B. et al. A single-cell molecular map of mouse gastrulation and early organogenesis. Nature 566, 490–495 (2019).
Nowotschin, S. et al. The emergent landscape of the mouse gut endoderm at single-cell resolution. Nature 569, 361–367 (2019).
Lim, J. & Thiery, J. P.Epithelial–mesenchymal transitions: insights from development. Development 139, 3471–3486 (2012).
Nieto, M. A., Huang, R. Y.-J., Jackson, R. A. & Thiery, J. P.EMT: 2016. Cell 166, 21–45 (2016).
Thiery, J. P., Acloque, H., Huang, R. Y. J. & Nieto, M. A.Epithelial–mesenchymal transitions in development and disease. Cell 139, 871–890 (2009).
Zorn, A. M. & Wells, J. M. Vertebrate endoderm development and organ formation. Annu. Rev. Cell Dev. Biol. 25, 221–251 (2009).
Wilkinson, D. G., Bhatt, S. & Herrmann, B. G.Expression pattern of the mouse T gene and its role in mesoderm formation. Nature 343, 657–659 (1990).
Ang, S. L. & Rossant, J. HNF-3β is essential for node and notochord formation in mouse development. Cell 78, 561–574 (1994).
Weinstein, D. C. et al. The winged-helix transcription factor HNF-3β is required for notochord development in the mouse embryo. Cell 78, 575–588 (1994).
Burtscher, I. & Lickert, H.Foxa2 regulates polarity and epithelialization in the endoderm germ layer of the mouse embryo. Development 136, 1029–1038 (2009).
Arnold, S. J. & Robertson, E. J.Making a commitment: cell lineage allocation and axis patterning in the early mouse embryo. Nat. Rev. Mol. Cell Biol. 10, 91–103 (2009).
Nowotschin, S., Hadjantonakis, A.-K. K. & Campbell, K.The endoderm: a divergent cell lineage with many commonalities. Development 146, dev150920 (2019).
Lindsley, R. C. et al. Mesp1 coordinately regulates cardiovascular fate restriction and epithelial–mesenchymal transition in differentiating ESCs. Cell Stem Cell 3, 55–68 (2008).
TextXKitajima, S., Takagi, A., Inoue, T. & Saga, Y.MesP1 and MesP2 are essential for the development of cardiac mesoderm. Development 127, 3215–3226 (2000).
Leptin, M.twist and snail as positive and negative regulators during Drosophila mesoderm development. Genes Dev. 5, 1568–1576 (1991).
Cano, A. et al. The transcription factor Snail controls epithelial–mesenchymal transitions by repressing E-cadherin expression. Nat. Cell Biol. 2, 76–83 (2000).
Carver, E. A., Jiang, R., Lan, Y., Oram, K. F. & Gridley, T. The mouse snail gene encodes a key regulator of the epithelial–mesenchymal transition. Mol. Cell Biol. 21, 8184–8188 (2001).
Peinado, H., Olmeda, D. & Cano, A.Snail, ZEB and bHLH factors in tumour progression: an alliance against the epithelial phenotype? Nat. Rev. Cancer 7, 415–428 (2007).
Lamouille, S., Xu, J. & Derynck, R. Molecular mechanisms of epithelial–mesenchymal transition. Nat. Rev. Mol. Cell Biol. 15, 178–196 (2014).
Hatta, K. & Takeichi, M. Expression of N-cadherin adhesion molecules associated with early morphogenetic events in chick development. Nature 320, 447–449 (1986).
Zohn, I. E. et al. p38 and a p38-interacting protein are critical for downregulation of E-cadherin during mouse gastrulation. Cell 125, 957–969 (2006).
Nieto, M. A., Bennett, M. F., Sargent, M. G. & Wilkinson, D. G. Cloning and developmental expression of Sna, a murine homologue of the Drosophila snail gene. Development 116, 227–237 (1992).
Beddington, R. S. P. & Robertson, E. J.Axis development and early asymmetry in mammals. Cell 96, 195–209 (1999).
Tam, P. P. L. et al. Sequential allocation and global pattern of movement of the definitive endoderm in the mouse embryo during gastrulation. Development 134, 251–260 (2006).
Tam, P. P. L. & Beddington, R. S. P.Establishment and organization of germ layers in the gastrulating mouse embryo. Ciba Found. Symp. 165, 27–41 (2007).
Rivera-Pérez, J. A. & Hadjantonakis, A. K.The dynamics of morphogenesis in the early mouse embryo. Cold Spring Harb. Perspect. Biol. 7, a015867 (2015).
Viotti, M., Nowotschin, S. & Hadjantonakis, A. K.SOX17 links gut endoderm morphogenesis and germ layer segregation. Nat. Cell Biol. 16, 1146–1156 (2014).
Burtscher, I., Barkey, W., Schwarzfischer, M., Theis, F. J. & Lickert, H.The Sox17–mCherry fusion mouse line allows visualization of endoderm and vascular endothelial development. Genesis 50, 496–505 (2012).
Burtscher, I., Barkey, W. & Lickert, H.Foxa2–Venus fusion reporter mouse line allows live-cell analysis of endoderm-derived organ formation. Genesis 51, 596–604 (2013).
Bergen, V., Lange, M., Peidli, S., Wolf, F. A. & Theis, F. J.Generalizing RNA velocity to transient cell states through dynamical modeling. Nat. Biotechnol. 38, 1408–1414 (2020).
Lange, M. et al. CellRank for directed single-cell fate mapping. Preprint at bioRxiv https://doi.org/10.1101/2020.10.19.345983 (2020).
Wang, P. et al. A molecular signature for purified definitive endoderm guides differentiation and isolation of endoderm from mouse and human embryonic stem cells. Stem Cells Dev. 21, 2273–2287 (2012).
Cernilogar, F. M. et al. Pre-marked chromatin and transcription factor co-binding shape the pioneering activity of Foxa2. Nucleic Acids Res. 47, 9069–9086 (2019).
Takagi, T., Moribe, H., Kondoh, H. & Higashi, Y.δEF1, a zinc finger and homeodomain transcription factor, is required for skeleton patterning in multiple lineages. Development 125, 21–31 (1998).
Liu, Y., El-Naggar, S., Darling, D. S., Higashi, Y. & Dean, D. C.Zeb1 links epithelial–mesenchymal transition and cellular senescence. Development 135, 579–588 (2008).
Zhou, B. P. et al. Dual regulation of Snail by GSK-3β-mediated phosphorylation in control of epithelial–mesenchymal transition. Nat. Cell Biol. 6, 931–940 (2004).
Artus, J. & Hadjantonakis, A. K.Generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos. Methods Mol. Biol. 693, 37–56 (2011).
Muzumdar, M. D., Tasic, B., Miyamichi, K., Li, N. & Luo, L.A global double-fluorescent Cre reporter mouse. Genesis 45, 593–605 (2007).
Haegel, H. et al. Lack of β-catenin affects mouse development at gastrulation. Development 121, 3529–3537 (1995).
Kemler, R. et al. Stabilization of β-catenin in the mouse zygote leads to premature epithelial–mesenchymal transition in the epiblast. Development 131, 5817–5824 (2004).
Ben-Haim, N. et al. The nodal precursor acting via activin receptors induces mesoderm by maintaining a source of its convertases and BMP4. Dev. Cell 11, 313–323 (2006).
Peinado, H., Quintanilla, M. & Cano, A.Transforming growth factor β-1 induces Snail transcription factor in epithelial cell lines: mechanisms for epithelial mesenchymal transitions. J. Biol. Chem. 278, 21113–21123 (2003).
Yook, J. I. et al. A Wnt–Axin2–GSK3β cascade regulates Snail1 activity in breast cancer cells. Nat. Cell Biol. 8, 1398–1406 (2006).
Jong, I. Y., Li, X. Y., Ota, I., Fearon, E. R. & Weiss, S. J.Wnt-dependent regulation of the E-cadherin repressor snail. J. Biol. Chem. 280, 11740–11748 (2005).
Hovanes, K. et al. β-catenin-sensitive isoforms of lymphoid enhancer factor-1 are selectively expressed in colon cancer. Nat. Genet. 28, 53–57 (2001).
Filali, M., Cheng, N., Abbott, D., Leontiev, V. & Engelhardt, J. F.Wnt-3A/β-catenin signaling induces transcription from the LEF-1 promoter. J. Biol. Chem. 277, 33398–33410 (2002).
Tamplin, O. J. et al. Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives. BMC Genomics 9, 511 (2008).
Cieply, B. et al. Suppression of the epithelial–mesenchymal transition by grainyhead-like-2. Cancer Res. 72, 2440–2453 (2012).
Chung, V. Y. et al. GRHL2–miR-200–ZEB1 maintains the epithelial status of ovarian cancer through transcriptional regulation and histone modification. Sci. Rep. 6, 19943 (2016).
Qi, X. K. et al. OVOL2 links stemness and metastasis via fine-tuning epithelial–mesenchymal transition in nasopharyngeal carcinoma. Theranostics 8, 2202–2216 (2018).
Du, J. et al. O-fucosylation of thrombospondin type 1 repeats restricts epithelial to mesenchymal transition (EMT) and maintains epiblast pluripotency during mouse gastrulation. Dev. Biol. 346, 25–38 (2010).
Yeung, T. L. et al. ELF3 is a negative regulator of epithelial–mesenchymal transition in ovarian cancer cells. Oncotarget 8, 16951–16963 (2017).
Benz, B. A. et al. Genetic and biochemical evidence that gastrulation defects in Pofut2 mutants result from defects in ADAMTS9 secretion. Dev. Biol. 416, 111–122 (2016).
Yang, J. et al. Guidelines and definitions for research on epithelial–mesenchymal transition. Nat. Rev. Mol. Cell Biol. 21, 341–352 (2020).
Townes, P. L. & Holtfreter, J.Directed movements and selective adhesion of embryonic amphibian cells. J. Exp. Zool. 128, 53–120 (1955).
Zheng, X. et al. Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer. Nature 527, 525–530 (2015).
Chen, Y. et al. Dual reporter genetic mouse models of pancreatic cancer identify an epithelial‐to‐mesenchymal transition‐independent metastasis program. EMBO Mol. Med. 10, e9085 (2018).
Song, Y., Washington, M. K. & Crawford, H. C.Loss of FOXA1/2 is essential for the epithelial-to-mesenchymal transition in pancreatic cancer. Cancer Res. 70, 2115–2125 (2010).
Wang, J. et al. FOXA2 suppresses the metastasis of hepatocellular carcinoma partially through matrix metalloproteinase-9 inhibition. Carcinogenesis 35, 2576–2583 (2014).
Tang, Y., Shu, G., Yuan, X., Jing, N. & Song, J.FOXA2 functions as a suppressor of tumor metastasis by inhibition of epithelial-to-mesenchymal transition in human lung cancers. Cell Res. 21, 316–326 (2011).
Fehling, H. J. et al. Tracking mesoderm induction and its specification to the hemangioblast during embryonic stem cell differentiation. Development 130, 4217–4227 (2003).
Hitz, C., Wurst, W. & Kühn, R.Conditional brain-specific knockdown of MAPK using Cre/loxP regulated RNA interference. Nucleic Acids Res. 35, e90 (2007).
Gegg, M. et al. Flattop regulates basal body docking and positioning in mono- and multiciliated cells. eLife 3, e03842 (2014).
Mfopou, J. K. et al. Efficient definitive endoderm induction from mouse embryonic stem cell adherent cultures: a rapid screening model for differentiation studies. Stem Cell Res. 12, 166–177 (2014).
Stephens, M.False discovery rates: a new deal. Biostatistics 18, 275–294 (2017).
La Manno, G. et al. RNA velocity of single cells. Nature 560, 494–498 (2018).
Wolf, F. A., Angerer, P. & Theis, F. J.SCANPY: large-scale single-cell gene expression data analysis. Genome Biol. 19, 15 (2018).
Hie, B., Bryson, B. & Berger, B.Efficient integration of heterogeneous single-cell transcriptomes using Scanorama. Nat. Biotechnol. 37, 685–691 (2019).
McInnes, L., Healy, J., Saul, N. & Großberger, L.UMAP: uniform manifold approximation and projection. J. Open Source Softw. 3, 861 (2018).
Blondel, V. D., Guillaume, J. L., Lambiotte, R. & Lefebvre, E. Fast unfolding of communities in large networks. J. Stat. Mech. 2008, P10008 (2008).
Wolf, F. A. et al. PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells. Genome Biol. 20, 59 (2019).
This work was supported by the Helmholtz-Gemeinschaft (Helmholtz Portfolio Theme ‘Metabolic Dysfunction and Common Disease’), Deutsches Zentrum für Diabetesforschung (DZD) and Helmholtz Alliance ‘Aging and Metabolic Programming’ (AMPro) (J.B., M.I. and H.L.). K.S. is supported by the HELENA Graduate School and Technische Universität München. This research was funded in part by Wellcome Trust grant 108413/A/15/D (F.J.T. and M.B.). For the purpose of open access, F.J.T. has applied a CC BY public copyright licence to any author accepted manuscript version arising from this submission. F.J.T. acknowledges support from the Helmholtz Association’s Initiative and Networking Fund through Helmholtz AI (grant number ZT-I-PF-5-01; F.J.T. and M.B.). Work in the laboratory of G.S. was funded by Deutsche Forschungsgemeinschaft grants SFB1064-A11 and SFB1321-P13 (G.S. and F.M.C.). We thank M. Bakhti for reading and discussing the manuscript; L. Appel, A. Ziegler, B. Vogel, J. Beckenbauer, W. Barkey and A. Bettenbrock for excellent technical assistance; and C. Hübner Freitag from the Helmholtz Core Facility Pathology & Tissue Analytics for excellent assistance with histology.
F.J.T. reports receiving consulting fees from and having an ownership interest in Cellarity.
Peer review information Nature Cell Biology thanks the anonymous reviewers for their contribution to the peer review of this work.
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Extended Data Fig. 1 The endoderm is formed independent of a full EMT-MET cycle.
a, Still images extracted from live cell imaging of a LS stage FVF/SCF embryo (representative of 4 embryos). The images show a single z-plane of the merge of the FVF channel in green and SCF channel in red. White dashed line shows the border of the epiblast, separating FVFlow and FVFhigh expressing cells. The yellow arrows show a single FVF cell that increases fluorescent intensity; red asterisks: DE progenitor upregulating Sox17 and intercalating into the DE layer. b, Immunohistochemistry of E6.25 (pre-streak) (2 embryos) and E6.5 (early-streak stage) (8 embryos) FVF/SCF embryos stained for Venus (Foxa2), E-cadherin and RFP (Sox17) showing Foxa2 expressing cells appearing before PS is present and (c) after PS induction (8 embryos) Foxa2 expressing cells are distal to the PS (blue dashed line indicates PS). d,e, Immunohistochemistry of LS stage FVF embryo (7 embryos) stained for Venus (Foxa2), Snail1 and T (d), LS stage wildtype embryo (3 embryos) stained for Foxa2, Snail1 and Sox17. Note, Foxa2high/Sox17+ TP does not express Snail1 (yellow arrowhead) (e). f, LS stage FVF embryo (3 embryos) immunostained for Venus (Foxa2), N-cadherin and E-cadherin. g, Quantification of Foxa2, T and Snail1 positive cells in MES, AME, Foxa2high TP of MS stage embryos (Ordinary one-way ANOVA with Tukey’s multiple comparison test, n = 4 embryos). h, Quantification of Foxa2, Sox17 and Snail1 expression in AME, Foxa2high TP/Foxa2high DE/VE and Foxa2high TP in MS stage embryos (Ordinary one-way ANOVA with Tukey’s multiple comparison test, n = 3 embryos). i, Quantification of E-cadherin and N-cadherin expression in FVF+, FVFhigh TP, FVFhigh DE/VE of MS stage embryos (Ordinary one-way ANOVA with Tukey’s multiple comparison test, n = 3 embryos). All samples are derived from biologically independent experiments. Data are presented as mean values ±SEM.; ****P < 0.0001, **P < 0.0019, *P < 0.0337. Statistically non-significant results are not indicated in the figure. All shown confocal images are single planes of a z-stack unless otherwise stated. Scale bar: 50 µm, insets 10 µm.
Extended Data Fig. 2 Mapping and single cell transcriptional profiling of gastrulation.
a, Scheme for FACS sorting of FVF embryos for scRNA-seq analysis (2 FVFneg, 3 FVFlow and 3 FVFhigh samples were used in the scRNAseq analysis). b, UMAP plot colored by cell population. c, Dotplot of tissue-specific marker genes (top brackets). Colors indicate expression level, dot size indicates fraction of cells expressing the gene. d, UMAP plot with RNA velocity arrows, colored by both FVF-sorting and presence/absence of Foxa2 mRNA. RNA velocity shows gene dynamics derived from abundance of unspliced and spliced mRNA molecules for each gene. e, Barplots of the fate probability for the FVF-sorted and Foxa2 mRNA positive or negative subpopulations of the pEpi (cell numbers from left to right, n = 308; n = 52; n = 287; n = 428; n = 1140). Bar height denotes mean fate probability per state and upper/lower whiskers indicate SEM. The higher a bar, the more likely is the indicated fate for this group of cells. All shown confocal images are single planes of a z-stack unless otherwise stated. Scale bar: 50 µm.
Extended Data Fig. 3 Characterization of endoderm and mesoderm in vitro.
a, Targeting strategy to generate TGFP/+; Foxa2tagRFP/+ mESC line. An available T-GFP knock-in mESCs line62 was targeted with the described Foxa2tagRFP construct. Transcriptional start region (TSR), untranslated region (UTR). b, Gating strategy of differentiated TGFP/+; Foxa2tagRFP/+ mESC line towards Foxa2+ (RFP+) endodermal cells and (c) T+ (GFP+) mesodermal cells. Differentiated cells were stained for CD24 and sorted according to their CD24 expression levels Foxa2+/CD24low, Foxa2+/CD24high, T+/CD24low and T+/CD24neg at day 2 and 4 of differentiation for global mRNA expression profile analysis.
Extended Data Fig. 4 Endoderm does not show hallmarks of EMT in vitro.
a, Endoderm differentiation scheme of TGFP/+; Foxa2tagRFP/+ mESC line. b, Confocal images of differentiated TGFP/+; Foxa2tagRFP/+ mESC line (representative of 3 independent differentiations) at day 3 stained for GFP (T), Foxa2 and Snail1. Note, the presence of T+, Snail1+ MES population (yellow box), Foxa2high/T+ AME progenitors (white box) as well as Foxa2low/high DE progenitors, recapitulating the observations in vivo. c, Differentiation scheme of FVF mESCs towards endoderm fate. d, Confocal images of differentiated FVF mESCs (representative of 2 independent differentiations) at day 3 stained for Venus (Foxa2, white), E-cadherin and N-cadherin. e, Scheme of endoderm differentiation. f, Representative FACS plot of Foxa2Venus/+ cells after 3 days of differentiation, followed by FACS sorting of Foxa2Venus neg, Foxa2Venus low, Foxa2Venus high for (g) western blot analysis (2 experiments) and (h) quantification of E-cadherin (n = 2 experiments). All samples are derived from biologically independent experiments. Data are presented as mean values. All shown confocal images are single planes of a z-stack unless otherwise stated. Scale bar: 50 µm, insets 10 µm.
Extended Data Fig. 5 Generation of Snail1 knockout mESCs.
a, Targeting strategy to generate Snail1 KO mESCs. b, 3′ end genotyping PCR (WT = 601 bp, KI = 529 bp) and 5′ end genotyping PCR (WT = 738 bp, KI = 657 bp) of the used Snail1 KO clones F11 and B4. c, Endoderm differentiation scheme of Snail1 KO mESCs. d, Confocal images of differentiated WT and Snail1 KO mESCs (representative of 2 independent differentiations) at day 3 stained against Snail1 and Foxa2. e, FACS analysis of differentiated WT and Snail1 KO mESCs (2 independent differentiations) at day 3 stained for Snail1 confirming the absence of Snail1 in the Snail1 KO mESCs line. f, FACS analysis of differentiated wildtype and Snail1 KO mESCs for Foxa2 and Sox17 expression (n = 5 (wildtype), n = 8 (Snail1 KO)). All samples are derived from biologically independent experiments. All shown confocal images are single planes of a z-stack unless otherwise stated. Scale bar: 50 µm, insets 10 µm.
Extended Data Fig. 6 Inhibition of Wnt signaling promotes endoderm differentiation.
a, Scheme of endoderm differentiation under normal conditions (control) and with the supplementation of DKK1 or IWP2. b, Immunohistochemistry (representative of 3 independent differentiations), (c) FACS analysis and (d) quantification of differentiated TGFP/+; Foxa2tagRFP/+ under control, DKK1 and IWP2 conditions stained for GFP (T), Foxa2 and Snail1 (Ordinary one-way ANOVA with Bonferroni’s multiple comparison test, n = 4 independent differentiations). e, Confocal images (representative of 3 independent differentiations), (f) FACS analysis and (g) quantification of TGFP/+; Foxa2tagRFP/+ mESC differentiated for 3 days under control, DKK1 or IWP2 conditions and stained for GFP (T), Foxa2 and Sox17 (Ordinary one-way ANOVA with Bonferroni’s multiple comparison test, n = 3 independent differentiations). All samples are derived from biologically independent experiments. Data are presented as mean values ±SEM. d, *P < 0.0146, **P < 0.0011, (g) *P < 0.0233. Statistically non-significant results are not indicated in the figure. All shown confocal images are single planes of a z-stack unless otherwise stated. Scale bar: 50 µm, insets 10 µm.
Extended Data Fig. 7 Epithelial cell plasticity drives endoderm formation.
a, UMAP plot colored by cell population. b, Stacked violin plots showing the gene expression distribution (columns) of AJ, TJ, AB polarity and metalloproteinases (MPs) genes in pEpi (n = 2215) versus Epi (n = 1929). Color corresponds to normalized median gene expression for each group. c, Quantification of E-cadherin (one-way ANOVA, n = 4 embryos) and Claudin7 (one-way ANOVA, n = 3 embryos) expression intensity at the anterior and posterior epiblast of MS stage embryos. d, Stacked violin plots showing the expression of TFs, AJ, TJ, AB polarity and metalloproteinases (MPs) genes in Foxa2+ pEpi (n = 616) versus Foxa2- pEpi (n = 1599). e, Immunostainings for Foxa2, Laminin and Collagen 4 of ES, (f) MS and (g) LS stage embryos (representative of 7 embryos used for (e-g). Yellow dashed line indicates PS. h, MS stage embryo (3 embryos) stained for Foxa2, Scribble and E-cadherin. All samples are derived from biologically independent experiments. Data are presented as mean values ±SEM.; **P < 0.0017, ***P < 0.0054. Statistically non-significant results are not indicated in the figure. All shown confocal images are single planes of a z-stack unless otherwise stated. Scale bar: 50 µm, insets 10 µm.
Supplementary Table 1: Foxa2 targets. Supplementary Table 2: List of primers used for cloning and genotyping. Supplementary Table 3: List of antibodies used for immunohistochemistry and FACS.
Supplementary Video 1
Supplementary Video 1: Live-cell imaging of a late-streak-stage FVF/SCF embryo. FVFlow epiblast progenitors upregulate the fluorescence intensity and leave the epiblast. FVFhigh transitory progenitors upregulate Sox17 expression and intercalate into the visceral endoderm/definitive endoderm layer.
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Scheibner, K., Schirge, S., Burtscher, I. et al. Epithelial cell plasticity drives endoderm formation during gastrulation. Nat Cell Biol 23, 692–703 (2021). https://doi.org/10.1038/s41556-021-00694-x
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