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CRISPR mutagenesis screening of mice

Functional genetic screening of mice and other mammals is exceedingly challenging. A CRISPR-based mutagenesis screen in mice has successfully revealed amino acids vital for protein function of the DND1 gene, missense mutations of which lead to defects in primordial germ cell development.

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The authors declare no competing interests.

Correspondence to Ophir Shalem.

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Fig. 1: Strategy for CRISPR-based mutagenesis screening of mice.