Recent advances in cell biology enable precise molecular perturbations. The spatiotemporal organization of cells and organisms, however, also depends on physical processes such as diffusion or cytoplasmic flows, and strategies to perturb physical transport inside cells are not yet available. Here, we demonstrate focused-light-induced cytoplasmic streaming (FLUCS). FLUCS is local, directional, dynamic, probe-free, physiological, and is even applicable through rigid egg shells or cell walls. We explain FLUCS via time-dependent modelling of thermoviscous flows. Using FLUCS, we demonstrate that cytoplasmic flows drive partitioning-defective protein (PAR) polarization in Caenorhabditis elegans zygotes, and that cortical flows are sufficient to transport PAR domains and invert PAR polarity. In addition, we find that asymmetric cell division is a binary decision based on gradually varying PAR polarization states. Furthermore, the use of FLUCS for active microrheology revealed a metabolically induced fluid-to-solid transition of the yeast cytoplasm. Our findings establish how a wide range of transport-dependent models of cellular organization become testable by FLUCS.
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The authors acknowledge support from the Max Planck Society, a DFG-financed DIPP fellowship for M.Mi., infrastructural support by the Hyman lab, and technical support from MPI-CBG light-microscopy and scientific computing facilities. The authors thank F. Decker and J. Brugués for egg extracts, H. Petzold for cell culture support, D.J. Dickinson and B. Goldstein for sharing transgenic C. elegans strains, and M. Zerial, D. Braun, P. Tomancak, F. Jülicher, A. Hyman, C. Hoege, J. Saenz, K. Subramanian, N. Maghelli and E. Knust for discussions and comments.
The authors declare that parts of the published work led to the application for a European patent.
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Light-induced thermoviscous flows in water and honey.
Directional transport perturbation in Xenopus laevis egg extract.
Dynamic and localized induction of flows inside C. elegans embryos.
Quantification of sub-millisecond temperature dynamics.
Viability of C. elegans embryos in response to dynamic laser-induced heating.
Flow-driven PAR-2 loading enhancement on the membrane in polarized C. elegans embryos.
Flow-driven dynamic translocation of the PAR-2 domain in a polarized C. elegans embryo.
Induced cytoplasmic flows drive flows of the actomyosin cortex.
PAR polarization of the C. elegans embryo is a bi-stable process.
Micro-rheological flow stimulus.
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Mittasch, M., Gross, P., Nestler, M. et al. Non-invasive perturbations of intracellular flow reveal physical principles of cell organization. Nat Cell Biol 20, 344–351 (2018). https://doi.org/10.1038/s41556-017-0032-9
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