A genomic appraisal of invasive Salmonella Typhimurium and associated antibiotic resistance in sub-Saharan Africa

Van Puyvelde, Sandra; de Block, Tessa; Sridhar, Sushmita; Bawn, Matt; Kingsley, Robert A.; Ingelbeen, Brecht; Beale, Mathew A.; Barbé, Barbara; Jeon, Hyon Jin; Mbuyi-Kalonji, Lisette; Phoba, Marie-France; Falay, Dadi; Martiny, Delphine; Vandenberg, Olivier; Affolabi, Dissou; Rutanga, Jean Pierre; Ceyssens, Pieter-Jan; Mattheus, Wesley; Cuypers, Wim L.; van der Sande, Marianne A. B.; Park, Se Eun; Kariuki, Simon; Otieno, Kephas; Lusingu, John P. A.; Mbwana, Joyce R.; Adjei, Samuel; Sarfo, Anima; Agyei, Seth O.; Asante, Kwaku P.; Otieno, Walter; Otieno, Lucas; Tahita, Marc C.; Lompo, Palpouguini; Hoffman, Irving F.; Mvalo, Tisungane; Msefula, Chisomo; Hassan-Hanga, Fatimah; Obaro, Stephen; Mackenzie, Grant; Deborggraeve, Stijn; Feasey, Nicholas; Marks, Florian; MacLennan, Calman A.; Thomson, Nicholas R.; Jacobs, Jan; Dougan, Gordon; Kariuki, Samuel; Lunguya, Octavie

doi:10.1038/s41467-023-41152-6

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Published: 23 October 2023

A genomic appraisal of invasive Salmonella Typhimurium and associated antibiotic resistance in sub-Saharan Africa

Nature Communications volume 14, Article number: 6392 (2023) Cite this article

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Abstract

Invasive non-typhoidal Salmonella (iNTS) disease manifesting as bloodstream infection with high mortality is responsible for a huge public health burden in sub-Saharan Africa. Salmonella enterica serovar Typhimurium (S. Typhimurium) is the main cause of iNTS disease in Africa. By analysing whole genome sequence data from 1303 S. Typhimurium isolates originating from 19 African countries and isolated between 1979 and 2017, here we show a thorough scaled appraisal of the population structure of iNTS disease caused by S. Typhimurium across many of Africa’s most impacted countries. At least six invasive S. Typhimurium clades have already emerged, with ST313 lineage 2 or ST313-L2 driving the current pandemic. ST313-L2 likely emerged in the Democratic Republic of Congo around 1980 and further spread in the mid 1990s. We observed plasmid-borne as well as chromosomally encoded fluoroquinolone resistance underlying emergences of extensive-drug and pan-drug resistance. Our work provides an overview of the evolution of invasive S. Typhimurium disease, and can be exploited to target control measures.

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Introduction

Invasive non-typhoidal Salmonella (iNTS) disease has emerged as a major cause of morbidity and mortality in sub-Saharan Africa (sSA)^1,2. There are an estimated 594,000 cases annually with a mean case fatality rate of 14.5%, while these incidence figures may be an underestimation as surveillance for iNTS disease is infrequent^3,4. This systemic disease predominantly occurs among infants and young children, children with malaria and malnutrition and individuals living with human immunodeficiency virus (HIV) infections². Data on iNTS disease in sSA are still relatively scarce due to limited surveillance for bloodstream infections across the continent but estimated incidences show high variability in regions and countries in sSA, as well as within countries^5,6.

Salmonella enterica serovar Typhimurium (S. Typhimurium) is the most common cause of iNTS disease in sSA⁷. While S. Typhimurium strains causing gastroenteritis globally predominantly fall into multilocus sequence types (MLST) ST19 and ST34⁸, strains causing bloodstream infections in sSA are highly associated with clade ST313. These African-associated ST313 broadly fall into distinct lineages known as 1 and 2, and both are associated with multidrug resistance (MDR)⁹. Epidemiological and genomic analysis has shown that lineage 1 emerged first and is predominantly confined to the East African region. Lineage 2 emerged later and has spread broadly across sSA, potentially driven by the HIV epidemic and the use of chloramphenicol^9,10. However, a strict division between invasive and non-invasive S. Typhimurium based on the MLSTs does not appear to represent the situation well. Invasive S. Typhimurium disease is not exclusively associated with ST313 in sSA, as in Kenya, ST19 S. Typhimurium have also been reported as a relatively common cause of such bloodstream infections¹¹ and non-lineage 1 and 2 ST313 S. Typhimurium isolates isolated in the UK are generally associated with gastro-intestinal disease and present no travel association to sSA¹².

ST313 isolates are typically MDR with some having acquired extended-spectrum beta-lactamase (ESBL) genes driving ceftriaxone resistance, whilst resistance to fluoroquinolones (FQ, both plasmid and chromosomally encoded) and more recently to azithromycin (AZI) is emerging^9,10,13,14. This development significantly limits treatment options for iNTS disease in low- and middle-income countries (LMICs)¹⁵.

Despite the significant burden of invasive S. Typhimurium disease in sSA, our understanding of the bacterial population structure and consequently epidemiology is poor, which contributes to the lack of appropriate disease interventions. Herein we report on a broad genomic analysis approach involving 1303 S. Typhimurium strains, isolated between 1979 and 2017 from 19 sSA countries, providing an optimal update following on from the initial pan-African study by Okoro et al.⁹. Our study yields a timely perspective on the distribution and spread of the invasive S. Typhimurium pandemic of sSA including an analysis of antimicrobial resistance, including hotspots defined as geographical foci where extensive drug resistant (XDR) isolates are emerging. These data will yield a better understanding of the disease and will support the targeting of interventions.

Results

Several independent clades of invasive S. Typhimurium have emerged in Africa

Although whole genome sequencing has been used to analyse iNTS isolates, previous work focused on regional data or on the ST313 clade. To obtain a pan-African perspective, we analysed whole genome sequencing data of 1302 African S. Typhimurium in the context of 117 non-African S. Typhimurium isolates, including eight African travel-associated isolates. The African isolates span 1979–2017 (Supplementary Fig. 1), with the greatest density of sampling between 2008 and 2017, when 73.8% (961 out of 1302) strains were isolated.

Our phylogenetic analysis captured more population diversity than previously reported^9,16, whereas two 313 lineages 1 and 2 were reported before to cause invasive S. Typhimurium infections, we identified 4 additional major ST19 lineages. The majority of isolates (n = 1005) form a tight cluster within ST313 lineage II (ST313-L2), including isolates from East, Central and West African countries (Fig. 1a and Supplementary Fig. 1). ST313 lineage I (ST313-L1) isolates were much less prevalent (n = 87) and originated predominantly from East African countries. An additional 258 African S. Typhimurium isolates belonged to ST19 and included four monophyletic clusters (Supplementary Fig. 3).

**Fig. 1: The distribution of invasive S. Typhimurium in Africa.**

We identified six major clades associated with invasive S. Typhimurium infections in sSA. Four invasive S. Typhimurium clades were ST19 (ST19-L1 to L4) while two clades were ST313-L1 and ST313-L2, respectively (Supplementary Fig. 4 and Fig. 1a). Most of the invasive S. Typhimurium clades were identified in multiple countries, suggesting a widespread distribution of these clades (Fig. 1b). The East African region showed the highest diversity of clades, with all six invasive S. Typhimurium clades being identified. At the country level, Kenya and DRC showed the highest diversity, each with 5 out of 6 of the invasive S. Typhimurium clades being identified. Notably, ST19-L2 and ST19-L4 were apparently older clades and were identified in the 1980s in Rwanda and DRC, while ST19-L4 isolates were still being observed among the recent samples and in other regions. ST19-L1 and ST19-L3 were predominantly observed in the 2010s (Fig. 1c). ST313-L1 was observed throughout the 1990s and until recently, while the ST313-L2 isolates bacame most dominant since 2001 and appear to be driving the current pandemic in sSA. The ST313-L2 clade was further classified in seven subclades, (Supplementary Figs. 5 and 6 and Fig. 2a) with each subclade associated to one country, i.e., DRC (n = 5), Kenya (n = 1) and Malawi (n = 1), which suggests local clonal expansions (Fig. 2b).

**Fig. 2: The distribution of invasive S. Typhimurium clade ST313-L2 in Africa.**

Continental spread of the current ST313-L2 clade occurred in 1994–1996

A representative selection of 252 ST313-L2 isolates (Supplementary Fig. 7) was subjected to a phylogeographical analysis in which the country of the ancestral strains was predicted to gather further information on the spread of invasive S. Typhimurium across sSA. Our analysis assigned the DRC as the most likely source of ST313-L2, with the most recent common ancestor (MRCA) predicted to have originated in 1980 (95% highest posterior density (HPD) confidence interval 1974–1986), refining the earlier prediction of the ST313-L2 MRCA from 1977 (95% HPD 1957–1988)⁹. We found no evidence that ST313-L2 was present anywhere outside the DRC until 1994, supported by probability rates >97% for ancestry nodes in that period.

From 1994 onwards, we observed five independent introductions from the DRC into the East African region (two times into Uganda in 1996, once to Malawi in 1996, once to Malawi in 2000 and once to Kenya in 1995, all observed in Fig. 3 when the most likely location of a branch changes from DRC to East Africa) and one major introduction in the West African region (to Ghana in 1994) which preceded its subsequent spread. We also identified further introductions from West to East Africa. In West Africa, we detected frequent transmission events across borders, including transmission to Malawi, whereas in East Africa, evidence for spread to neighbouring countries was less apparent (Fig. 3 and Supplementary Fig. 8).

**Fig. 3: Estimated spread of ST313-L2 across sub-Saharan Africa.**

The ten ST313-L2 UK isolates could be directly linked to eight separate transmission events from Africa¹², underlining the importance of travel-associated transmissions of invasive S. Typhimurium.

Multiple chromosomal SNPs conferring resistance to ciprofloxacin have occurred in invasive S. Typhimurium

Acquisitions of chromosomal SNPs in quinolone resistance-determining regions (QRDR) were observed across the population and in the different regions of sSA (Supplementary Fig. 9). A total of 17 independent QRDR SNP acquisition events were observed in the African invasive S. Typhimurium clades, resulting in substitutions of GyrA S83F (n = 3), GyrA S83Y (n = 4), GyrA D87G (n = 1), GyrA D87N (n = 3), GyrA D87Y (n = 5), GyrB E466Y (n = 1) (Table 1). Eight of these QRDR SNP acquisition events gave rise to clusters of multiple isolates, suggesting local outbreaks. Three of these were observed in Kenya, three in DRC and two in Ghana. One of the Kenyan clusters contained isolates from 2005 to 2014, while one DRC cluster contained 49 isolates isolated between 2013 and 2017 without interruption, both suggesting a long-term circulation of the decreased ciprofloxacin susceptibility (DCS) clusters possibly linked to an ongoing outbreak.

Table 1 Quinolone resistance-determining region (QRDR) SNP acquisitions in African invasive S. Typhimurium clades

Full size table

The earliest observation of DCS was in 2002 (DRC, Lwiro), with subsequent acquisitions being continuously observed since then in East (Kenya), Central (DRC) and West (Benin, Ghana) Africa (Fig. 4). QRDR SNP acquisitions originated from different regions across the country and were not notably linked to a single local hotspot in Kenya or DRC (Table 1).

**Fig. 4: Continuous emergence of individual genetic events of quinolone resistance-determining regions (QRDR) SNP acquisitions in African invasive S. Typhimurium clades over time.**

The large GyrA S83Y cluster in ST313-L2 subclade 7 (n = 49) clade showed nested phylogenetic substructures, with significantly increased MIC values for ciprofloxacin in the emerging substructures (P value = 0.0235), starting at a median MIC of 0.125 mg/L and mean MIC of 0.132 mg/L and reaching a median MIC of 0.190 mg/L and mean MIC of 0.188 mg/L (Supplementary Fig. 2). No known genetic mechanisms for ciprofloxacin resistance were identified that explain these subtle increases in ciprofloxacin MIC values. However, non-synonymous SNPs were acquired in virulence gene speC, nitroreductase gene nfsA, SBOV13191, ydhZ, ethanolamine reactivase gene eutA and malate synthase gene aceB, none of which have been implicated in ciprofloxacin resistance before.

XDR and PDR invasive S. Typhimurium is associated with the presence of IncHI2 and IncI1 plasmids

Isolates that carried genetic markers underlying XDR or PDR, including ESBL activity, FQ and AZI resistance, were scattered across the phylogeny (Supplemental Fig. 10). XDR and PDR were observed in four different combinations (Fig. 5). Our data revealed that XDR has emerged at least six times in invasive S. Typhimurium, with one emergence underlying ST313-L2 subclade 3¹⁰. XDR was associated with either acquisition of AZI resistance (mphA) and ESBL (SHV) markers, or by acquired FQ resistance (qnr) and ESBL (CTX-M) markers. PDR, on the other hand, was observed three independent times, each time as a combination of AZI resistance (mphA), ESBL (SHV) and FQ resistance markers, which was either a qnr acquisition, chromosomal GyrA D87N or GyrB S464Y substitutions. Strikingly, XDR or PDR isolates always harboured an acquired plasmid: XDR coincided with either the presence of IncHI1 (n = 4) or IncHI2 (n = 50) replicons, while PDR was associated with the presence of IncHI2 plasmids (n = 3). The IncHI2 plasmid conserved in ST313-L2 subclade 3 carries the XDR resistance markers and was found to have high similarity with the IncHI2 plasmids of ST313-L2 isolates from Kenya and Malawi¹⁰.

**Fig. 5: Observed molecular mechanisms of extensive drug resistance (XDR) and pan-drug resistance (PDR) in invasive S. Typhimurium.**

We resolved the full plasmid sequence of all available XDR and PDR isolates and subjected them to a comparative analysis (overview in Table 2). IncHI2 plasmids contributing to XDR and PDR in invasive S. Typhimurium isolates in sSA were highly related to each other, but also to non-XDR/PDR IncHI2 plasmids (Fig. 6a). High similarity was, for example, observed with a plasmid from a non-invasive isolate from Morocco (S15BD01306) carrying mobilised colistin resistance gene (mcr-9). IncI1 plasmids contributing to XDR in invasive S. Typhimurium isolates in sSA were similarly highly related to each other, but also to other IncI1 plasmids (Fig. 6b).

Table 2 IncI1 and IncHI2 plasmids associated with antimicrobial resistance (AMR) identified in African invasive S. Typhimurium isolates

Full size table

**Fig. 6: Related IncHI2 and IncI1 plasmid drive XDR and PDR in African invasive S. Typhimurium.**

All XDR and PDR isolates originate from the DRC, and were found in multiple provinces (Sankuru, Tshopo [Kisangani], Kongo-Central [Kisantu]), suggesting a widespread of the resistance plasmids in the country. Related IncI1 and IncHI2 plasmids were however identified across Africa, i.e., respectively in Nigeria and Kenya, and in Kenya, Malawi and Morocco, which suggests a possible widespread reservoir of these types of plasmids and thus AMR across the continent. Strikingly, the historical isolate AA00491 (DRC, 1984) carried a hybrid IncI1-IncHI2 plasmid showing similarity to both the recent IncI1 and IncHI2 plasmids.

Hotspots for AMR in S. Typhimurium are found in Kenya and DRC

PDR and XDR previously had only been detected in DRC while ESBL production and QRDR SNP acquisitions were observed across sSA. PDR was observed in all three sites in DRC with extensive bloodstream surveillance; Kinshasa, Kisangani and Kisantu in the DRC. However, in Kisangani, Kisantu, Kinshasa, as well as in Nairobi (Kenya), isolates with XDR or ESBL-producing plasmids co-circulated with isolates showing QRDR SNPs, presenting a risk for PDR through plasmid transfer (Fig. 7).

**Fig. 7: Antimicrobial resistance (AMR) hotspots for invasive S. Typhimurium in sub-Saharan Africa.**

Most frequently, isolates with increased AMR presented as single, sporadic cases, although in the Kisantu hospital area in the DRC, both an XDR outbreak (ST313-L2 subclade 3) and a clade with a QRDR SNP (ST313-L2 subclade 7) were observed. These outbreaks of ST313-L2 subclades 3 and 7 underly two separate waves with increased cases, respectively from 2013 Q4 to 2015 Q1 and 2016 Q1 to 2017 Q3 (Supplementary Fig. 11).

Evolutionary processes in invasive S. Typhimurium clades

Invasive ST313 isolates have previously been associated with signatures of genome degradation associated with host adaptation¹⁷. To assess this here, non-synonymous SNPs were extracted, and the genes potentially inactivated in the invasive S. Typhimurium clades were analysed (Supplementary Table 1). Twenty-six such genes were identified in at least two invasive S. Typhimurium clades, and thus present parallel evolution towards loss of these genes in the separate invasive S. Typhimurium clades. Three of these genes were found in three of the six invasive S. Typhimurium clades, i.e., malZ (ST19-L2, ST19-L3 and ST313-L1), ssrA (ST19-L3, ST313-L1 and ST313-L2) and stfC (ST19-L4, ST313-L1 and ST313-L2). These genes are involved in virulence in a gut or macrophage environment^{18,19,20,21,22} or survival outside the host²³. Twenty-three genes showed non-synonymous SNPs in two of the six invasive S. Typhimurium clades and were related to metabolism (pdxK, eutA), host interactions/virulence (shdA, steC), membrane/surface-associated processes (dacD, fimA) redox-associated processes (ydiJ, ccmH2, bcp, SL1344_1475) or phage (SL1344_2600, SL1344_2582). Of these, none have previously been found as pseudogenes in human-associated S. Typhimurium. However, fimA has been found as a pseudogene in Bordetella pertussis²⁴, ydiD and shdA are known pseudogenes in S. Typhi and other host-adapted serovars^25,26,27, and steC is a pseudogene in some passerine-adapted S. Typhimurium^28,29. Other molecular mechanisms, such as subtle changes in expression of expression pumps might also underly the observed differences in MIC values.

When analysed within a global S. Typhimurium context, the six invasive S. Typhimurium clades from sSA presented different characteristics than global S. Typhimurium isolates, which would suggest that they have spread differently and have undergone different levels of host adaptation (Fig. 8). Overall, the invasive S. Typhimurium clades form distinct clades in the global phylogeny. Their difference from global S. Typhimurium isolates underlines their specific importance for sSA rather than across the globe, with however, few global isolates interspersed with ST19-L1 and ST19-L4 isolates.

**Fig. 8: Phylogenetic relationship of invasive S. Typhimurium clades in the global population structure of S. Typhimurium.**

We further investigated the genome degradation of lineages using the DBS, which is a measure for Salmonella invasiveness based on the content of pseudogenes in the isolate’s genomes. S. Typhimurium lineages belonging to global clade-α³⁰ had a low DBS, indicative for little genome degradation and lower signatures of invasiveness, and included ST19-L1 and ST19-L2 with DBSs of −0.0089 and 0.07, respectively (Fig. 8b). The lack of genome degradation in ST19-L1 was consistent with this, containing strains with a broad host range. In contrast, ST19-L2 had an elevated DBS, suggesting greater host adaptation, although there might be sampling bias or a local outbreak underlying these data as the ST19-L2 isolates were sampled over a short time frame at only one location. S. Typhimurium lineages belonging to global clade-β³⁰ had a wider range of DBSs. ST19-L3 and ST19-L4 (both ST19) had a mean DBS of 0.0337 and 0.0013, respectively. The high DBS of ST19-L3 is consistent with a highly degraded genome, considered to be a signature for increased invasiveness and similar to that described in the neighbouring DT56 complex. ST19-L4 has emerged from the DT204 complex that caused an MDR epidemic in Europe during the 1980s³¹. ST313-L1 and ST313-L2 are part of clade-β and present moderate to high genome degradation with mean DBSs of 0.0105 and 0.0197.

Discussion

We present an overview of invasive S. Typhimurium in sSA, maximally using a geographically and temporally balanced dataset of 1302 African isolates. Okoro et al. previously identified two phylogenetic ST313 lineages (called 1 and 2) as drivers of the S. Typhimurium epidemic in sSA⁹. We identified four further significant clades and underlined the importance of ST313-L2 in the current pandemic. Our data identified similar evolutionary signatures in each clade involving the acquisition of multiple AMR determinants and signs of genome degradation, potentially linked to host adaptation. Two of the ST19 clades were observed recently to be dominant in Kenya and were called the Kenyan ST19 lineages I and II¹¹, coinciding with ST19-L3 and ST19-L4. The other two ST19 clades described here, have not been described earlier. Another recent study has identified a novel clade in Malawi, likely presenting a seventh emergence of an invasive S. Typhimurium clade¹⁶. We present an updated numbering system to annotate those different invasive S. Typhimurium lineages with ST313-L2 as the current dominant clade in sSA, associated with highest levels of AMR. The emergence of multiple, independent lineages across the S. Typhimurium population, presenting similar evolutionary patterns towards invasiveness, implies that S. Typhimurium, ST19 and ST313, have the intrinsic capability of becoming invasive. In addition, as long as these drivers are present, one can assume that it is likely that new invasive lineages will continue to appear.

A limitation to this and other iNTS genomics studies is the scarcity of bacterial isolates available from bloodstream infections in sSA, associated with intrinsic sampling bias. First, healthcare utilisation is relatively low and frequently delayed in sSA. Second, blood culture analysis and surveillance are increasingly performed across sSA but are still not routinely done in all countries as it is expensive and poorly implemented in clinical care. Lastly, sample storage and sequencing are not systematically done on all samples. Whilst this situation is changing, this study is therefore opportunistic but nonetheless covers multiple regions across sSA, combining data from blood culture surveillance projects undertaken across sSA in the recent decades. The intrinsic sample bias however implies that patterns, such as the diversity of clades, that we observed between regions might be skewed. We have however taken steps throughout the study to mitigate these effects, including the use of rigourous statistical methods, to present the best possible predictions.

We postulate that the current pandemic ST313-L2 clade emerged in the DRC in 1980 and has spread across sSA from the DRC. Historical invasive S. Typhimurium isolates also have only been observed and reported in DRC and Rwanda. Although this might be a causal effect of the clade only being present in Central Africa, sample scarity and bias could be a limitation for this analysis.

The success of ST313-L2 has earlier been contributed to its chloramphenicol resistance⁹. Chloramphenicol was used frequently in DRC as first choice treatment for bacterial meningitis and typhoid fever³², and we observe chloramphenicol resistance in ST19-L4 from Lwiro, DRC isolated in 1984–1985. The main transmission events of ST313-L2 at the continental level happened in a time frame of only 2 years. Coincidently, major wars took place in de DRC during this period being the First Congo War or Africa’s First World War (1996–1997) and the Second Congo War or the Great African War (1998–2003) took place. During that time, there was substantial travel due to important foreign involvement and there were large numbers of refugees in the DRC after the Rwandese War (1994)³³. At the same time, the HIV pandemic was still expanding in sSA, creating a large susceptible population to iNTS infections⁹. Political instability may, therefore, have played a role in the intracontinental spread of invasive S. Typhimurium, which has also been seen for other diseases such as cholera³⁴.

Higher-order subclades present in ST313-L2 were confined to specific countries, suggesting a slow local spread of iNTS at the country level. Two subclades with increased AMR have emerged in DRC, namely one presenting XDR and one DCS, again implying that DRC might be a source for emerging subpopulations. Similarly, one ESBL ST313-L2 sub-branch emerged from Kenya, a country also presenting a high diversity of invasive S. Typhimurium clades and suggesting that Kenya might be another hotspot for emerging subpopulations. Four specific locations were identified as hotspots where IncHI2-plasmid-driven resistance coincides with chromosomal DCS (Kisantu, Kisangani and Kinshasa in DRC; Nairobi in Kenya). It will be important in the coming years to maintain genomic surveillance in these sites, and others. At the moment, single PDR cases have been reported in DRC¹⁰. Acquisition of XDR and PDR involved plasmids highly related to the IncHI2 and IncI1 plasmids, suggesting the existence of a mobile AMR-reservoir for S. Typhimurium in sSA. These IncHI2 and IncI1 plasmids are commonly found in the S. enterica species³⁵, but also in other related organisms such as Escherichia coli³⁶, with which the invasive S. Typhimurium isolates might exchange plasmids.

The increase of AMR is worrying in S. Typhimurium, especially with limited antimicrobials available and resistance being observed against all available antimicrobials. iNTS vaccines are currently in development³⁷, but it is however unlikely for these to become licensed and WHO prequalified before the end of the 2020 s. In addition, specific risk factors for iNTS infection (malnutrition, HIV and malaria co-infections) still are significant in the human population. There has never been a clinical endpoint trial of the management of iNTS disease and there is an urgent need for holistic approaches to prevention combining novel treatment strategies and preventive measures, including vaccine development and meeting Sustainable Development Goals around water, sanitation and hygiene (WASH), nutrition and control of malaria and HIV in endemic regions. Our study supports and urges for further action by providing a timely and continental perspective of invasive S. Typhimurium and its AMR in sSA.

Methods

Bacterial isolates

A total of 1419 S. Typhimurium isolates were included in this study, containing 1302 isolates from Africa. These originated from surveillance studies in 19 countries spanning different subregions of Africa (East, Central and West) (Supplementary Data 1 and Supplementary Fig. 1). Of these African isolates, 115 (8.1%) isolates were part of the study by Okoro⁹ providing the S. Typhimurium overview in 2012, and including the ST313 lineage II D23580 isolate, used here as reference genome³⁸. An additional 815 (57.4%) isolates were sequenced as part of the studies with isolates from sSA from Van Puyvelde et al.^10,39, Post et al.⁴⁰, Kariuki et al.^11,13,41, Feasey et al.⁴², Msefula et al.⁴³, Park et al.¹⁴ and Maclennan et al. (in preparation). As part of this study, 372 (26.2%) additional isolates from sSA were whole genome sequenced. Of these, three isolates originate from bacterial surveillance in the Centre National Hospitalier Universitaire Hubert Koutougou MAGA of Cotonou, Benin; 33 (2.3%) originate from bloodstream surveillance studies and human reservoir and transmission studies conducted in the Clinical Research Unit of Nanoro (CRUN) in Burkina Faso, 134 (9.4%) originate from the Democratic Republic of Congo (DRC), including ongoing bloodstream surveillance^44,45,46,47 and historical isolates which were bio-banked in the University Hospital Saint-Pierre Brussels (Belgium)^48,49,50,51; 3 (0.2%) isolates originate from returning travellers from Gabon, Guinea and Morocco stored by Sciensano (Belgium); 86 (6.0%) isolates originate from the Malawi Liverpool Welcome (MLW) bacteraemia archive and dedicated stool samplings by the MLW Research Programme in Blantyre, Malawi⁵²; 29 (2.0%) isolates from Nigeria originate from the Community Acquired Bacteremic Syndrome in Young Nigerian Children CABSYNC study and the Community Acquired Pneumonia and Invasive Bacterial Disease (CAPIBD), 69 (4.9%) from Rwanda of which one recent isolate collected by the Rwandan National Reference Laboratory in Kigali (Rwanda) and 68 historical isolates which were bio-banked in the University Hospital Saint-Pierre Brussels (Belgium)^48,49,50,51; 15 (1.1%) isolates originate from population-based surveillance by the Medical Research Council (MRC) Unit The Gambia at the London School of Hygiene & Tropical Medicine (LSHTM) in the Basse region of The Gambia⁵³. We have included the 75 (5.3%) published sequences from Ashton et al.¹², including ST313 sequences isolated by Public Health England in the UK, as context. Information on the sample source was available for 1102 of the African S. Typhimurium isolates, and include abattoir (1; 0.1%) human blood (1006; 91.4%), stool (84; 7.6%), urine (4; 0.4%), pus (1; 0.1%), cerebrospinal fluid (4; 0.4%) and unspeccified human sample (1; 0.1%). Sixty-one of the 84 African stool isolates originate from endemic settings and presented invasive clones in the stool samples^11,54. All available information on the isolates is available in Supplementary Data 1.

Genome sequencing

Each of the collaborating laboratories used their own individual methodologies for the extraction of genomic DNA. Index-tagged paired-end Illumina sequencing libraries were prepared as previously described⁵⁵. These were combined into pools of 96 uniquely tagged libraries and sequenced on the Illumina HiSeq 2000 or HiSeq 2500 platform (San Diego, USA) according to the manufacturer’s protocols to generate paired-end reads of 100–150 bp in length. Sequencing read quality was confirmed for all samples, including the presence of sequencing adapters, contamination level, GC fraction, insert size, qX yield, match with reference genomes and coverage of mapping, using the sequencing pipelines (Wellcome Sanger Institute, Hinxton, UK). Purity of the samples was confirmed by sorting reads per species for each sample using Kraken v.1.1.1⁵⁶. Samples were assembled using Velvet v.1.2.10⁵⁷ and needed to cover at least 4.5 Mbp of the genome. Detailed statistics of the individual assemblies, including the number of contigs, average contig lenthg, max contig length and N50 are given in Supplementary Data 2.

Seven isolates from this study were subjected to MinION sequencing to resolve their plasmid sequences (Supplementary Data 3). These included all isolates which were available for additional sequencing and which showed high AMR (XDR, PDR and/or ESBL). For monophyletic branches with the same AMR profile and plasmid content, one isolate was subjected to MinION sequencing. DNA for MinION sequencing was extracted using the MasterPure Complete DNA and RNA Purification Kit (Epicentre, Madison, USA), following the manufacturer’s guidelines. Nuclease-free water (Thermo Fisher scientific, Waltham, USA) was used to resuspend the DNA. Genomic DNA extracts were natively barcoded (with EXP-NBD104, SQK-LSK109) and sequenced on a MinION using flowcell type R9.4.1. The sequence was determined from the raw fast5 output files using guppy basecaller in high accuracy mode (default parameters with --config dna_r9.4.1_450bps_hac.cfg, Guppy basecalling suite, (C) Oxford Nanopore Technologies, Limited. v3.0.3) to obtain fastq files. These fastq files were demultiplexed with guppy barcoder v3.0.3 and porechop v0.2.3 (default parameters with --barcode_kit EXP-NBD104). Hybrid assemblies from Nanopore and Illumina read sequences were constructed using Unicycler v0.4.6⁵⁸ and the assembly graphs were evaluated using Bandage⁵⁹. Sequences were then annotated using PROKKA v1.11⁶⁰.

In silico identification of AMR and plasmid replicons

To detect AMR genes, the ARIBA software v.2.14.6⁶¹ with default thresholds and CARD database v.3.0.7⁶² were used. For the detection of plasmids, the PlasmidFinder⁶³ database was used with the mapping-based allele tool SRST2 v.0.2.0⁶⁴.

MDR was defined as the co-presence of genetic markers conferring resistance to chloramphenicol, ampicillin and co-trimoxazole; XDR as the co-presence of genetic markers which are responsible for the resistance to two of the three current treatment options (AZI, FQ or ceftriaxone) on top of MDR; pan-drug resistance (PDR) was defined as co-presence of genetic markers conferring resistance to AZI, FQ and ceftriaxone combined with MDR^10,15.

SNP analysis

Illumina HiSeq reads were mapped to the S. Typhimurium reference genomes of ST313 lineage II (D23580, FN424405.1³⁸) using SMALT v0.7.4 to produce a BAM file. SMALT was used to index the reference using a kmer size of 20 and a step size of 13, and the reads were aligned using default parameters but with the maximum insert size set as three times the mean fragment size of the sequencing library. PCR duplicate reads were identified using Picard v1.92 (Broad Institute, Cambridge, MA, USA) and flagged as duplicates in the BAM file.

Variation detection was performed using samtools mpileup v0.1.19 with parameters “-d 1000 -DSugBf” and bcftools v0.1.19⁶⁵ to produce a BCF file of all variant sites. The option to call genotypes at variant sites was passed to the bcftools call. All bases were filtered to remove those with uncertainty in the base call. The bcftools variant quality score was required to be greater than 50 and mapping quality greater than 30. If not all reads gave the same base call, the allele frequency, as calculated by bcftools, was required to be either 0 for bases called the same as the reference, or 1 for bases called as a SNP. The majority base call was required to be present in at least 75% of reads mapping at the base, and the minimum mapping depth required was 4 reads, at least two of which had to map to each strand. Finally, strand_bias was required to be less than 0.001, map_bias less than 0.001 and tail_bias less than 0.001. If any of these filters were not met, the base was called as uncertain.

Phylogenetic analysis

A pseudo-genome was constructed by substituting the base call at each site (variant and non-variant) in the BCF file into the reference genome and any site called as uncertain was substituted with an N. Insertions with respect to the reference genome were ignored and deletions with respect to the reference genome were filled with N’s in the pseudo-genome to keep it aligned and the same length as the reference genome used for read mapping.

Recombinant regions in the chromosome such as prophage regions and the fljB ORF in the chromosome were removed from the alignment and checked using Gubbins v1.4.10⁶⁶. SNP sites were extracted from the alignment using snp-sites v.2.5.1⁶⁷ and used to construct a maximum likelihood phylogeny with RAxML v8.2.8⁶⁸ with substitution model GTR-GAMMA. For the full tree, support for nodes on the trees was assessed using 100 bootstrap replicates and S. Paratyphi A270 was included as an outgroup to root the tree. For the ST313-L2 tree, 1000 bootstrap replicates were used and isolate S. Typhimurium DT2 was included as an outgroup to root the tree. Trees were visualised using Figtree v1.4.2 and iTOL⁶⁹.

Identification of clades and subclades

For the identification of clades and subclades within the African S. Typhimurium population, a similar approach was used as previously employed for the classification of S. Typhi⁷⁰. FastBaps v.1.0.3⁷¹ was used with the ‘baps’ prior. Hierarchal clustering analysis was run on the full dataset allowing identification of six clades, here defined as invasive Typhimurium clades ST19-L1–4 and ST313-L1-2. Clade ST313-L2 isolates (formerly defined as ST313 sublineage II) were separately subjected to hierarchal clustering thereby yielding seven subclades (ST313-L2 subclades 1–7). Hereto, ten nested levels of molecular variation were fitted to the data and level 2 allowed identification clusters until single-member clusters thereby following the approach used for S. Typhi classification⁷², and the level 2 classification was further used for the (sub)clade definitions. The hierarchal clustering method is a phylogeny-free approach, and population structure was taken into account by integrating level 2 clusters with the phylogenetic trees.

Clades were defined as FastBaps clusters presenting as monophyletic branches. Neighbouring and nested clusters were merged in one clade while the differentiating branch showed a minimum of 50 SNPs difference. Neighbouring clusters were merged until the next cluster was dominated by non-African non-invasive isolates, allowing a representative classification of invasive African S. Typhimurium isolates in 6 major clades. This results in 21 of the 118 non-African isolates being clustered within the invasive S. Typhimurium clades. Unclassified isolates were assigned to the ancestry clade (Supplementary Data 1).

Subclades of ST313-L2 were defined similarly as clades, i.e., as FastBaps clusters presenting as monophyletic branches. All neighbouring and nested clusters were merged in subclades, with the differentiating branch showing at least 4 SNPs difference. Merging of neighbouring clusters was continued as far as possible. To maintain compatibility with the phylogeny, few single nested isolates needed to be reassigned. Some isolates did not reach the criteria for clustering as they were part of polyphyletic lineages and were assigned to the ancestry subclade of ST313-L2 This approach uses stringent settings, thereby limiting classification of all potential substructures of ST313-L2 but provides a framework with significant subclades and a nomenclature which can be extended in the future.

Evolutionary context analysis

To determine the evolutionary context of the African strains, 10 representatives randomly selected from the phylogeny (Supplementary Data 4) of each invasive S. Typhimurium clade were placed in a phylogenetic context with strains from a well-characterised dataset of 131 S. Typhimurium strains³⁰ (Supplementary Data 5). Maximum likelihood phylogenetic trees were constructed as previously described³⁰. Briefly, reads were mapped to the SL1344 whole genome sequence assembly (FQ312003) and SNPs were determined using the Rapid haploid variant calling and core SNP phylogeny pipeline SNIPPY v.3.0 (https://github.com/tseemann/snippy). The software was also used to construct a sequence alignment of core genome variant sites from which a maximum likelihood phylogenetic tree was generated using the GTRCAT model implemented with an extended majority-rule consensus tree criterion in RAxML⁷³. The Delta Bitscore (DBS) was calculated for the proteome inferred from each genome to estimate the level of genome degradation as previously described³⁰. Briefly, reads were mapped to the SL1344 reference genome to create alignment sequences which were then annotated using Prokka v1.11⁶⁰. Genes in each annotated sequence were then analysed in a pairwise fashion against SL1344 using DBS⁷⁴ as described previously³⁰. The mean DBS per genome was plotted.

Phylogeography

A representative selection of ST313-L2 isolates was made by first stratifying the dataset by year and using CD-HIT v.4.8.1⁷⁵ at a 0.99 threshold per group of isolates to identify isolates representing the genetic diversity within the group. A total of 220 isolates were selected using this approach and complemented with 31 isolates which were selected based on the RAxML phylogeny and present additional geographical transmission events in the maximum likelihood tree. Reference isolate D23580 was also included.

A temporal signal was identified in the dataset with TempEST v.1.5.3⁷⁶. The phylogeography was reconstructed using BEAST v1.8.4⁷⁷. BEAUti xml’s were constructed to compare the different substitution models (GTR + I, GTR+Gamma), molecular clocks (Strict, Relaxed Log-Normal) and population size models (Constant, Exponential, Logistic, Expansion, Skygrid, Sky Ride, Bayesian Skyline, Extended Bayesian Skyline). The ratio of the marginal likelihoods were compared using Bayes factor^78,79 and yielded the general time reversible (GTR) substitution model with diffuse gamma distribution prior (shape 0.001, scale 1000) and invariant sites, an uncorrelated log-normal relaxed molecular clock and a model with exponential population size as optimal. Countries were added as traits in the BEAST model, and ancestors states were reconstructed at all ancestors.

Three independent runs of 80 million MCMC generations were calculated and samples were taken every 8000 generations. Log files were inspected in Tracer v1.7.1⁸⁰ for convergence, proper mixing, and sufficient sampling. A 3.75% burn-in was removed from each run. Log and tree files were combined using LogCombiner v2.5.0. The posterior sample of the time-trees were summarised in TreeAnnotator v1.8.4 to produce a maximum clade credibility tree with the posterior estimates of node heights visualised on it.

We inferred transmission events along branches where the predicted country changed between internal nodes in the final BEAST maximum credibility tree. These data were used to generate the inferred transmissions shown on the map. For the phylogeographical analysis, the Bayes_factor_comparison.py, replace_BEAST_blocks.py and prepare_BEAST_alignment.py scripts were used, available at Github page https://github.com/sanger-pathogens/bact-gen-scripts.

Plasmid comparisons

XDR and PDR IncI1 and IncHI2 plasmids were pairwise compared using BRIG v.0.95⁸¹. The respective IncHI2 and IncI1 plasmids that were complete, circular and had the longest sequence were used as references for comparison, which were IncHI2 plasmid pSTM-10530_17 (296484 bp) and IncI1 plasmid pSTM-23060_3 (97430 bp). Included plasmid sequences obtained through this study using MinION sequences were IncHI2 plasmids pSTM-10530_17, pSTM-3152_4, pSTM-5390_4, pSTM7593_12 and IncI1 plasmids pSTM-23060_3, pSTM-22392_3, pSTM-22400_3, pSTM-8892. Public sequences of IncHI2 plasmids pSTm-ST313-II.1 (S. Typhimurium, ERS1310131), pKST313 (S. Typhimurium, LN794248) and pSTm-A54650 (S. Typhimurium, LK056646) were included.

A de novo assembly was performed based on Illumina sequences of isolates AA00491, S15BD01306, K171 and 8892⁸² and the contigs of the plasmid sequence were identified using a pairwise blastN v. 2.10.0 analysis of these sequences with respectively pSTM-10530_17 and pSTM_23060_3 as the query. All contigs covering more than 3% of the query sequence were retained for this analysis. Contigs were reordered using Mauve version 2015_02_25⁸³.

Ciprofloxacin susceptibility testing and statistical analysis

Antimicrobial susceptibility of the DRC isolates was done before⁴⁷, following CLSI guidelines by assessing Minimum Inhibitory Concentrations (MIC) with the E-test macromethod (bioMérieux, Oxoid) for ciprofloxacin. An increase of MIC values per nested substructure of S. Typhimurium ST313-L2 subclade 7 was statistically analysed using a generalised linear model (glm function) using R software, with a Gaussian distribution. The nested substructures are given in Supplementary Fig. 2a.

Ethics statement

Ethical approval for the Microbiological Surveillance was granted by the Institutional Review Board of the Institute of Tropical Medicine, Anwerp (ref. 613/08, 23/03/2021 and ref. 1108/16, 23/08/2016), by the Ethics Committees of the Antwerp University Hospital, Belgium (ref. 08172613, 01/04/2021 and ref. 16/34/347, 09/01/2017) and the School of Public Health in Kinshasa, DRC (ref. ESP/CE/092/2021, 12/05/2021), University of Malawi College of Medicin Research Ethics Committee (COMREC P.06/20/3071), the Rwanda National Ethics Committee (ref. 903/RNEC/2018, 17/12/2018), the Comité Local d’Ethique Pour la Recherche Biomédicale de Parakou, Benin (0195/CLERB-UP/P/SP/R/SA), the Gambia Government/Medical Research Council Unit The Gambia Joint Ethics Committee (ref. 1087). Research ethical approval was granted by the Ethical Review Boards of the Centre de Recherche en Science Naturelle (CRSN) of Lwiro, DRC and the Kenya Medical Research Institute Scientific and Ethics Review Unit (KEMRI/SERU)-KEMRI/SERU/CGHR/005/3055, the Ethics committee of the Federal Capital Territory FHREC/2012/01/11/16-05-12 and the Ethics committee of Aminu Kano Teaching Hospital-NHREC/21/08/2008/AKTH/EC/1633 and the Health Services Management Board of Kano State- 2/1437AH-9/12/2015.

Reporting summary

Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.

Data availability

Sequence data that support the findings of this study are available at SRA, accession IDs per isolate are available in Supplementary Data 1 and 3. All data generated during and/or analysed in the current study are available from the corresponding author on request. The exchange of biological material should always be in agreement with the local teams.

References

Feasey, N. A., Dougan, G., Kingsley, R. A., Heyderman, R. S. & Gordon, M. A. Invasive non-typhoidal Salmonella disease: an emerging and neglected tropical disease in Africa. Lancet 379, 2489–2499 (2012).
Article PubMed PubMed Central Google Scholar
Gilchrist, J. J. & MacLennan, C. A. Invasive nontyphoidal Salmonella disease in Africa. EcoSal Plus 8, 10–1128 (2019).
Stanaway, J. D. et al. Collaborators GBDN-TSID. The global burden of non-typhoidal Salmonella invasive disease: a systematic analysis for the Global Burden of Disease Study 2017. Lancet Infect. Dis. 19, 1312–1324 (2019).
(IHME) IfHMaE. in GBD 2019 Cause and Risk Summary: Invasive non-typhoidal Salmonella (iNTS). (eds) (Global Health Metrics, 2021). https://www.healthdata.org/results/gbd_summaries/2019/invasive-non-typhoidal-salmonella-ints-level-3-cause.
Marks, F. et al. Incidence of invasive Salmonella disease in sub-Saharan Africa: a multicentre population-based surveillance study. Lancet Glob. Health 5, e310–e323 (2017).
Article PubMed PubMed Central Google Scholar
Marchello, C. S., Fiorino, F., Pettini, E. & Crump, J. A. Vacc-i NTSCC. Incidence of non-typhoidal Salmonella invasive disease: a systematic review and meta-analysis. J. Infect. 83, 523–532 (2021).
Article PubMed PubMed Central Google Scholar
Uche, I. V., MacLennan, C. A. & Saul, A. A systematic review of the incidence, risk factors and case fatality rates of invasive nontyphoidal salmonella (iNTS) disease in Africa (1966 to 2014). PLoS Negl. Trop. Dis. 11, e0005118 (2017).
Article PubMed PubMed Central Google Scholar
Branchu, P., Bawn, M. & Kingsley, R. A. Genome variation and molecular epidemiology of Salmonella enterica Serovar Typhimurium pathovariants. Infect. Immun. 86, 10–1128 (2018).
Okoro, C. K. et al. Intracontinental spread of human invasive Salmonella Typhimurium pathovariants in sub-Saharan Africa. Nat. Genet. 44, 1215–1221 (2012).
Article CAS PubMed PubMed Central Google Scholar
Van Puyvelde, S. et al. An African Salmonella Typhimurium ST313 sublineage with extensive drug-resistance and signatures of host adaptation. Nat. Commun. 10, 4280 (2019).
Article PubMed PubMed Central ADS Google Scholar
Kariuki, S. et al. High relatedness of invasive multi-drug resistant non-typhoidal Salmonella genotypes among patients and asymptomatic carriers in endemic informal settlements in Kenya. PLoS Negl. Trop. Dis. 14, e0008440 (2020).
Article CAS PubMed PubMed Central Google Scholar
Ashton, P. M. et al. Public health surveillance in the UK revolutionises our understanding of the invasive Salmonella Typhimurium epidemic in Africa. Genome Med. 9, 92 (2017).
Article PubMed PubMed Central Google Scholar
Kariuki, S. et al. Ceftriaxone-resistant Salmonella enterica serotype Typhimurium sequence type 313 from Kenyan patients is associated with the blaCTX-M-15 gene on a novel IncHI2 plasmid. Antimicrob. Agents Chemother. 59, 3133–3139 (2015).
Article CAS PubMed PubMed Central Google Scholar
Park, S. E. et al. The genomic epidemiology of multi-drug resistant invasive non-typhoidal Salmonella in selected sub-Saharan African countries. BMJ Glob. Health 6, e005659 (2021).
Tack, B., Vanaenrode, J., Verbakel, J. Y., Toelen, J. & Jacobs, J. Invasive non-typhoidal Salmonella infections in sub-Saharan Africa: a systematic review on antimicrobial resistance and treatment. BMC Med. 18, 212 (2020).
Article PubMed PubMed Central Google Scholar
Pulford, C. V. et al. Stepwise evolution of Salmonella Typhimurium ST313 causing bloodstream infection in Africa. Nat. Microbiol. 6, 327–338 (2020).
Okoro, C. K. et al. Signatures of adaptation in human invasive Salmonella Typhimurium ST313 populations from sub-Saharan Africa. PLoS Negl. Trop. Dis. 9, e0003611 (2015).
Article PubMed PubMed Central Google Scholar
Tapio, S., Yeh, F., Shuman, H. A. & Boos, W. The malZ gene of Escherichia coli, a member of the maltose regulon, encodes a maltodextrin glucosidase. J. Biol. Chem. 266, 19450–19458 (1991).
Article CAS PubMed Google Scholar
Laudisi, F. et al. The food additive maltodextrin promotes endoplasmic reticulum stress-driven mucus depletion and exacerbates intestinal inflammation. Cell Mol. Gastroenterol. Hepatol. 7, 457–473 (2019).
Article PubMed Google Scholar
Mulder, D. T. et al. Multiple histidines in the periplasmic domain of the Salmonella enterica sensor kinase SsrA enhance signaling in response to extracellular acidification. Mol. Microbiol. 95, 678–691 (2015).
Article CAS PubMed Google Scholar
Julio, S. M., Heithoff, D. M. & Mahan, M. J. ssrA (tmRNA) plays a role in Salmonella enterica serovar Typhimurium pathogenesis. J. Bacteriol. 182, 1558–1563 (2000).
Article CAS PubMed PubMed Central Google Scholar
Boyen, F. et al. A limited role for SsrA/B in persistent Salmonella Typhimurium infections in pigs. Vet. Microbiol. 128, 364–373 (2008).
Article CAS PubMed ADS Google Scholar
Kroupitski, Y. et al. Identification of Salmonella enterica genes with a role in persistence on lettuce leaves during cold storage by recombinase-based in vivo expression technology. Phytopathology 103, 362–372 (2013).
Article CAS PubMed Google Scholar
Willems, R. J., van der Heide, H. G. & Mooi, F. R. Characterization of a Bordetella pertussis fimbrial gene cluster which is located directly downstream of the filamentous haemagglutinin gene. Mol. Microbiol. 6, 2661–2671 (1992).
Article CAS PubMed Google Scholar
Bronner, D. N. et al. Genetic ablation of butyrate utilization attenuates gastrointestinal Salmonella disease. Cell Host Microbe 23, 266–273.e264 (2018).
Article CAS PubMed PubMed Central Google Scholar
Campbell, J. W., Morgan-Kiss, R. M. & Cronan, J. E. Jr. A new Escherichia coli metabolic competency: growth on fatty acids by a novel anaerobic beta-oxidation pathway. Mol. Microbiol 47, 793–805 (2003).
Article CAS PubMed Google Scholar
Betancor, L. et al. Genomic comparison of the closely related Salmonella enterica Serovars Enteritidis and Dublin. Open Microbiol. J. 6, 5–13 (2012).
Article PubMed PubMed Central Google Scholar
Fu, Y., Smith, J. C., Shariat, N. W., M’Ikanatha, N. M. & Dudley, E. G. Evidence for common ancestry and microevolution of passerine-adapted Salmonella enterica serovar Typhimurium in the UK and USA. Microb. Genom. 8, 000775 (2022).
Cohen, E. et al. Pathoadaptation of the passerine-associated Salmonella enterica serovar Typhimurium lineage to the avian host. PLoS Pathog. 17, e1009451 (2021).
Article CAS PubMed PubMed Central Google Scholar
Bawn, M. et al. Evolution of Salmonella enterica serotype Typhimurium driven by anthropogenic selection and niche adaptation. PLoS Genet. 16, e1008850 (2020).
Article CAS PubMed PubMed Central Google Scholar
Rabsch, W., Tschape, H. & Baumler, A. J. Non-typhoidal salmonellosis: emerging problems. Microbes Infect. 3, 237–247 (2001).
Article CAS PubMed Google Scholar
Mashako, M. N. et al. [Salmonella infections in Kinshasa: the species involved and sensitivity to antibiotics]. Pediatrie 46, 691–696 (1991).
CAS PubMed Google Scholar
Flahaux, M. & Schoumaker, B. in Democratic Republic of the Congo: A Migration History Marked by Crises and Restrictions. (eds) (Migration Policy Institute, 2016). https://www.migrationinstitute.org/publications/democratic-republic-of-the-congo-a-migration-history-marked-by-crises-and-restrictions.
Weill, F. X. et al. Genomic history of the seventh pandemic of cholera in Africa. Science 358, 785–789 (2017).
Article CAS PubMed ADS Google Scholar
Emond-Rheault, J. G. et al. The Salmonella enterica plasmidome as a reservoir of antibiotic resistance. Microorganisms 8, 1016 (2020).
Wong, V. K. et al. Phylogeographical analysis of the dominant multidrug-resistant H58 clade of Salmonella typhi identifies inter- and intracontinental transmission events. Nat. Genet. 47, 632–639 (2015).
Article CAS PubMed PubMed Central Google Scholar
Tennant, S. M., MacLennan, C. A., Simon, R., Martin, L. B. & Khan, M. I. Nontyphoidal Salmonella disease: current status of vaccine research and development. Vaccine 34, 2907–2910 (2016).
Article CAS PubMed Google Scholar
Kingsley, R. A. et al. Epidemic multiple drug resistant Salmonella Typhimurium causing invasive disease in sub-Saharan Africa have a distinct genotype. Genome Res. 19, 2279–2287 (2009).
Article CAS PubMed PubMed Central Google Scholar
Van Puyvelde, S. et al. Genetic and structural variation in the O-antigen of Salmonella enterica Serovar Typhimurium isolates causing bloodstream infections in the Democratic Republic of the Congo. mBio 13, e0037422 (2022).
Article PubMed Google Scholar
Post, A. S. et al. Supporting evidence for a human reservoir of invasive non-Typhoidal Salmonella from household samples in Burkina Faso. PLoS Negl. Trop. Dis. 13, e0007782 (2019).
Kariuki, S. & Onsare, R. S. Epidemiology and genomics of invasive nontyphoidal Salmonella infections in Kenya. Clin. Infect. Dis. 61, S317–S324 (2015).
Article CAS PubMed PubMed Central Google Scholar
Msefula, C. L. et al. Genotypic homogeneity of multidrug resistant S. Typhimurium infecting distinct adult and childhood susceptibility groups in Blantyre, Malawi. PLoS ONE 7, e42085 (2012).
Article CAS PubMed PubMed Central ADS Google Scholar
Feasey, N. A. et al. Drug resistance in Salmonella enterica ser. Typhimurium bloodstream infection, Malawi. Emerg. Infect. Dis. 20, 1957–1959 (2014).
Article CAS PubMed PubMed Central Google Scholar
Kalonji, L. M. et al. Invasive Salmonella infections at multiple surveillance sites in the Democratic Republic of the Congo, 2011-2014. Clin. Infect. Dis. 61, S346–S353 (2015).
Article PubMed Google Scholar
Ley, B. et al. Invasive Salmonella enterica serotype Typhimurium infections, Democratic Republic of the Congo, 2007-2011. Emerg. Infect. Dis. 20, 701–704 (2014).
Article PubMed PubMed Central Google Scholar
Lunguya, O. et al. Antimicrobial resistance in invasive non-typhoid Salmonella from the Democratic Republic of the Congo: emergence of decreased fluoroquinolone susceptibility and extended-spectrum beta lactamases. PLoS Negl. Trop. Dis. 7, e2103 (2013).
Article PubMed PubMed Central Google Scholar
Tack, B. et al. Non-typhoidal Salmonella bloodstream infections in Kisantu, DR Congo: emergence of O5-negative Salmonella Typhimurium and extensive drug resistance. PLoS Negl. Trop. Dis. 14, e0008121 (2020).
Article PubMed PubMed Central Google Scholar
Lepage, P. et al. Severe multiresistant Salmonella Typhimurium systemic infections in Central Africa–clinical features and treatment in a paediatric department. J. Antimicrob. Chemother. 14, 153–159 (1984).
Article PubMed Google Scholar
Lepage, P. et al. Metastatic focal infections due to multiresistant Salmonella Typhimurium in children: a 34 month experience in Rwanda. Eur. J. Epidemiol. 2, 99–103 (1986).
Article CAS PubMed Google Scholar
Lepage, P. et al. Community-acquired bacteraemia in African children. Lancet 1, 1458–1461 (1987).
Article CAS PubMed Google Scholar
Vandenberg, O. et al. Microbiologic and clinical features of Salmonella species isolated from bacteremic children in eastern Democratic Republic of Congo. Pediatr. Infect. Dis. J. 29, 504–510 (2010).
Article PubMed Google Scholar
Feasey, N. A. et al. Three epidemics of invasive multidrug-resistant Salmonella bloodstream infection in Blantyre, Malawi, 1998-2014. Clin. Infect. Dis. 61, S363–S371 (2015).
Article PubMed PubMed Central Google Scholar
Gambia Pneumococcal Surveillance, G. et al. Impact of the introduction of pneumococcal conjugate vaccination on invasive pneumococcal disease and pneumonia in The Gambia: 10 years of population-based surveillance. Lancet Infect. Dis. 21, 1293–1302 (2021).
Article Google Scholar
Post, A. S. et al. Supporting evidence for a human reservoir of invasive non-typhoidal Salmonella from household samples in Burkina Faso. PLoS Negl. Trop. Dis. 13, e0007782 (2019).
Article PubMed PubMed Central Google Scholar
Croucher, N. J. et al. Rapid pneumococcal evolution in response to clinical interventions. Science 331, 430–434 (2011).
Article CAS PubMed PubMed Central ADS Google Scholar
Wood, D. E. & Salzberg, S. L. Kraken: ultrafast metagenomic sequence classification using exact alignments. Genome Biol. 15, R46 (2014).
Article PubMed PubMed Central Google Scholar
Zerbino, D. R. & Birney, E. Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res. 18, 821–829 (2008).
Article CAS PubMed PubMed Central Google Scholar
Wick, R. R., Judd, L. M., Gorrie, C. L. & Holt, K. E. Unicycler: resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput. Biol. 13, e1005595 (2017).
Article PubMed PubMed Central ADS Google Scholar
Wick, R. R., Schultz, M. B., Zobel, J. & Holt, K. E. Bandage: interactive visualization of de novo genome assemblies. Bioinformatics 31, 3350–3352 (2015).
Article CAS PubMed PubMed Central Google Scholar
Seemann, T. Prokka: rapid prokaryotic genome annotation. Bioinformatics 30, 2068–2069 (2014).
Article CAS PubMed Google Scholar
Hunt, M. et al. ARIBA: rapid antimicrobial resistance genotyping directly from sequencing reads. Micro. Genom. 3, e000131 (2017).
Google Scholar
McArthur, A. G. et al. The comprehensive antibiotic resistance database. Antimicrob. Agents Chemother. 57, 3348–3357 (2013).
Article CAS PubMed PubMed Central Google Scholar
Carattoli, A. et al. In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing. Antimicrob. Agents Chemother. 58, 3895–3903 (2014).
Article PubMed PubMed Central Google Scholar
Inouye, M. et al. SRST2: rapid genomic surveillance for public health and hospital microbiology labs. Genome Med. 6, 90 (2014).
Article PubMed PubMed Central Google Scholar
Li, H. et al. The sequence Alignment/Map format and SAMtools. Bioinformatics 25, 2078–2079 (2009).
Article PubMed PubMed Central Google Scholar
Croucher, N. J. et al. Rapid phylogenetic analysis of large samples of recombinant bacterial whole genome sequences using Gubbins. Nucleic Acids Res. 43, e15 (2015).
Article PubMed Google Scholar
Page, A. J. et al. SNP-sites: rapid efficient extraction of SNPs from multi-FASTA alignments. Micro. Genom. 2, e000056 (2016).
Google Scholar
Stamatakis, A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30, 1312–1313 (2014).
Article CAS PubMed PubMed Central Google Scholar
Letunic, I. & Bork, P. Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Res. 44, W242–W245 (2016).
Article CAS PubMed PubMed Central Google Scholar
Wong, V. K. et al. An extended genotyping framework for Salmonella enterica serovar Typhi, the cause of human typhoid. Nat. Commun. 7, 12827 (2016).
Article CAS PubMed PubMed Central ADS Google Scholar
Tonkin-Hill, G., Lees, J. A., Bentley, S. D., Frost, S. D. W. & Corander, J. Fast hierarchical Bayesian analysis of population structure. Nucleic Acids Res. 47, 5539–5549 (2019).
Article CAS PubMed PubMed Central Google Scholar
Corander, J., Marttinen, P., Siren, J. & Tang, J. Enhanced Bayesian modelling in BAPS software for learning genetic structures of populations. BMC Bioinforma. 9, 539 (2008).
Article Google Scholar
Stamatakis, A. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22, 2688–2690 (2006).
Article CAS PubMed Google Scholar
Wheeler, N. E., Barquist, L., Kingsley, R. A. & Gardner, P. P. A profile-based method for identifying functional divergence of orthologous genes in bacterial genomes. Bioinformatics 32, 3566–3574 (2016).
Article CAS PubMed PubMed Central Google Scholar
Li, W. & Godzik, A. Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences. Bioinformatics 22, 1658–1659 (2006).
Article CAS PubMed Google Scholar
Rambaut, A., Lam, T. T., Max Carvalho, L. & Pybus, O. G. Exploring the temporal structure of heterochronous sequences using TempEst (formerly Path-O-Gen). Virus Evol. 2, vew007 (2016).
Article PubMed PubMed Central Google Scholar
Drummond, A. J., Suchard, M. A., Xie, D. & Rambaut, A. Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol. Biol. Evol. 29, 1969–1973 (2012).
Article CAS PubMed PubMed Central Google Scholar
Baele, G., Li, W. L., Drummond, A. J., Suchard, M. A. & Lemey, P. Accurate model selection of relaxed molecular clocks in Bayesian phylogenetics. Mol. Biol. Evol. 30, 239–243 (2013).
Article CAS PubMed Google Scholar
Baele, G. et al. Improving the accuracy of demographic and molecular clock model comparison while accommodating phylogenetic uncertainty. Mol. Biol. Evol. 29, 2157–2167 (2012).
Article CAS PubMed PubMed Central Google Scholar
Rambaut, A., Drummond, A. J., Xie, D., Baele, G. & Suchard, M. A. Posterior summarization in Bayesian phylogenetics using tracer 1.7. Syst. Biol. 67, 901–904 (2018).
Article CAS PubMed PubMed Central Google Scholar
Alikhan, N. F., Petty, N. K., Ben Zakour, N. L. & Beatson, S. A. BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons. BMC Genom. 12, 402 (2011).
Article CAS Google Scholar
Page, A. J. et al. Robust high-throughput prokaryote de novo assembly and improvement pipeline for Illumina data. Micro. Genom. 2, e000083 (2016).
Google Scholar
Darling, A. C., Mau, B., Blattner, F. R. & Perna, N. T. Mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res. 14, 1394–1403 (2004).
Article CAS PubMed PubMed Central Google Scholar

Download references

Acknowledgements

We are grateful to Jacqueline Keane, Christoph Puethe and the Pathogen Informatics team (Wellcome Sanger Institute, Hinxton, Cambridge, United Kingdom) for the support. The work by S.V.P. and G.D. is funded in part by a grant from the Bill & Melinda Gates Foundation (OPP1151153). R.K. and M.B. were supported by research grants BB/N007964/1 and BB/M025489/1, and by the BBSRC Institute Strategic Programme Microbes in the Food Chain BB/R012504/1 and its constituent projects BBS/E/F/000PR10348 and BBS/E/F/000PR10349. W.L.C. was supported by the Research Foundation—Flanders (FWO SB PhD fellowship 1S40018N); J.P.R. was financially supported by the Belgian Directorate General for Development Cooperation (DGD). M.A.B. and N.R.T. were supported by Wellcome funding to the Sanger Institute (#206194). The work done in Benin, Burkina Faso and DRC by B.B., L.M.-K., M.-F.P., D.F., D.A., J.J. and O.L. was funded by the Belgian Directorate of Development Cooperation (DGD) through the Multi-Year Programme (2012–2016) between the Belgian DGD and the Institute of Tropical Medicine, Belgium and (for DRC) by the Baillet-Latour find and the Flemish Interuniversity Council (VLIR-UOS). The isolates from Malawi were generated by Malawi Liverpool Wellcome Research Programme bacteraemia service, supported by Asia and Africa Programme Grant 206545/Z/17/Z to NF. The work in The Gambia was supported by the Bill & Melinda Gates Foundation (OPP1020327); GAVI The Vaccine Alliance’s Accelerated Development and Introduction Plan (PneumoADIP), Medical Research Council (UK) to GM. Salmonella isolates obtained through the RTS,S study was funded by the Bill & Melinda Gates Foundation to CAM. Salmonella isolates obtained through the TSAP study were funded by the Bill & Melinda Gates Foundation to IVI (OPPGH5231) to F.M., H.J.J. and S.E.P. This research by S.V.P., S.S. and G.D. was funded by the National Institute for Health Research [Cambridge Biomedical Research Centre at the Cambridge University Hospitals NHS Foundation Trust]. The views expressed are those of the authors and not necessarily those of the NHS, the NIHR or the Department of Health and Social Care. This research was funded in whole, or in part, by the Wellcome Trust (#206194).

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These authors contributed equally: Gordon Dougan, Samuel Kariuki, Octavie Lunguya.

Authors and Affiliations

Cambridge Institute of Therapeutic Immunology and Infectious Disease, University of Cambridge School of Clinical Medicine, Cambridge Biomedical Campus, Cambridge, CB2 0AW, UK
Sandra Van Puyvelde, Sushmita Sridhar, Hyon Jin Jeon, Florian Marks & Gordon Dougan
Parasites and Microbes Programme, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK
Sandra Van Puyvelde, Sushmita Sridhar, Mathew A. Beale & Nicholas R. Thomson
Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, University of Antwerp, Antwerp, Belgium
Sandra Van Puyvelde
Institute of Tropical Medicine, Antwerp, Belgium
Tessa de Block, Brecht Ingelbeen, Barbara Barbé, Jean Pierre Rutanga, Wim L. Cuypers, Marianne A. B. van der Sande, Stijn Deborggraeve & Jan Jacobs
Division of Infectious Disease, Massachusetts General Hospital, Boston, MA, USA
Sushmita Sridhar
Department of Medicine, Harvard Medical School, Boston, MA, USA
Sushmita Sridhar
Quadram Institute Bioscience, Norwich, UK
Matt Bawn & Robert A. Kingsley
Earlham Institute, Norwich, UK
Matt Bawn
Faculty of Biological Sciences, University of Leeds, Leeds, UK
Matt Bawn
School of Biological Science, University of East Anglia, Norwich, UK
Robert A. Kingsley
Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands
Brecht Ingelbeen & Marianne A. B. van der Sande
International Vaccine Institute, 1 Gwanak-ro, Seoul, 08826, Republic of Korea
Hyon Jin Jeon, Se Eun Park & Florian Marks
Madagascar Institute for Vaccine Research, University of Antananarivo, Antananarivo, Madagascar
Hyon Jin Jeon & Florian Marks
Department of Medical Biology, University Teaching Hospital of Kinshasa, Kinshasa, Democratic Republic of the Congo
Lisette Mbuyi-Kalonji, Marie-France Phoba & Octavie Lunguya
National Institute for Biomedical Research, Kinshasa, Democratic Republic of the Congo
Lisette Mbuyi-Kalonji, Marie-France Phoba & Octavie Lunguya
Department of Pediatrics, University Hospital of Kisangani, Kisangani, Democratic Republic of the Congo
Dadi Falay
Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), Université Libre de Bruxelles (ULB), 1000, Brussels, Belgium
Delphine Martiny & Olivier Vandenberg
Faculty of Medicine and Pharmacy, University of Mons (UMONS), 7000, Mons, Belgium
Delphine Martiny
Division of Infection and Immunity, Faculty of Medical Sciences, University College London, London, UK
Olivier Vandenberg
Centre National Hospitalier Universitaire Hubert Koutoukou Maga, Cotonou, Benin
Dissou Affolabi
College of Science and Technology, University of Rwanda, Kigali, Rwanda
Jean Pierre Rutanga
National Reference Center for Salmonella, Unit of Human Bacterial Diseases, Sciensano, J. Wytsmanstraat 14, B-1050, Brussels, Belgium
Pieter-Jan Ceyssens & Wesley Mattheus
Department of Computer Science, University of Antwerp, Antwerp, Belgium
Wim L. Cuypers
Yonsei University Graduate School of Public Health, Seodaemun-gu, Seoul, 03722, Republic of Korea
Se Eun Park
Kenya Medical Research Institute/Centre for Global Health Research, Kisumu, Kenya
Simon Kariuki & Kephas Otieno
National Institute for Medical Research, Tanga, Tanzania
John P. A. Lusingu & Joyce R. Mbwana
Center for Medical Parasitology, Department of Immunology and Microbiology, University of Copenhagen, København, Denmark
John P. A. Lusingu
University of Health & Allied Sciences, Ho, Volta Region, Ghana
Samuel Adjei, Anima Sarfo & Seth O. Agyei
Kintampo Health Research Centre, Research and Development Division, Ghana Health Service, Kintampo North Municipality, Ho, Volta Region, Ghana
Kwaku P. Asante
KEMRI/Walter Reed Project, Kombewa, Kenya
Walter Otieno & Lucas Otieno
Institut de Recherche en Science de la Santé, Direction Régionale du Centre-Ouest/ClinicalResearch Unit of Nanoro, Nanoro, Burkina Faso
Marc C. Tahita & Palpouguini Lompo
University of North Carolina Project, Lilongwe, Malawi
Irving F. Hoffman, Tisungane Mvalo & Nicholas Feasey
Department of Pediatrics, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
Tisungane Mvalo
Malawi Liverpool Wellcome Research Programme, Kamuzu University of Health Sciences, Blantyre, Malawi
Chisomo Msefula
Department of Paediatrics, Bayero University, Kano, Nigeria
Fatimah Hassan-Hanga
Aminu Kano Teaching Hospital, Kano, Nigeria
Fatimah Hassan-Hanga
University of Nebraska Medical Center, Omaha, NE, USA
Stephen Obaro
International Foundation Against Infectious Diseases in Nigeria (IFAIN), Abuja, Nigeria
Stephen Obaro
Medical Research Council Unit The Gambia at London School of Hygiene & Tropical Medicine, Fajara, The Gambia
Grant Mackenzie
London School of Hygiene and Tropical Medicine, Keppel St, Bloomsbury, London, WC1E 7HT, UK
Grant Mackenzie & Nicholas R. Thomson
Murdoch Children’s Research Institute, Melbourne, VIC, Australia
Grant Mackenzie
Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, UK
Nicholas Feasey
Heidelberg Institute of Global Health, University of Heidelberg, Heidelberg, Germany
Florian Marks
The Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK
Calman A. MacLennan
Enteric and Diarrheal Diseases, Global Health, Bill & Melinda Gates Foundation, Seattle, WA, USA
Calman A. MacLennan
Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium
Jan Jacobs
Centre for Microbiology Research, Kenya Medical Research Institute, Nairobi, Kenya
Samuel Kariuki

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Sandra Van Puyvelde
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Tessa de Block
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Matt Bawn
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Robert A. Kingsley
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Hyon Jin Jeon
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Contributions

Study design and oversight: S.V.P., G.D., Sa.K. and O.L. Isolate collection and metadata: B.B., H.J.J., L.M.-K., M.-F.P., D.F., D.M., O.V., D.A., J.P.R., P.-J.C., W.M., S.E.P., Si.K., K.O., J.P.A.L., J.R.M., S.A., A.S., S.O.A., K.P.A., W.O., L.T.O., M.C.T., P.L., I.F.H., T.M., C.M., F.-H.H., S.O., G.M., N.F., F.M., C.A.M., J.J., Sa.K. and O.L. Sequencing: S.V.P., Td.B., S.D. and N.R.T. Data analysis: S.V.P., Td.B., S.S., M.B., R.A.K., B.I., M.A.B., W.L.C., M.A.B.v.d.S. and N.R.T. Writing—original draft preparation: S.V.P. and G.D. Writing—a critical review: S.V.P., Td.B., S.S., M.B., R.A.K., B.I., M.A.B., B.B., H.J.J., L.M.-K., M.-F.P., D.F., D.M., O.V., D.A., J.P.R., P.-J.C., W.M., W.L.C., M.A.B.v.d.S., S.E.P., Si.K., K.O., J.P.A.L., J.R.M., S.A., A.S., S.O.A., K.P.A., W.O., L.T.O., M.C.T., P.L., I.F.H., T.M., C.M., F.H.-H., S.O., G.M., S.D., N.F., F.M., C.A.M., N.R.T., J.J., G.D., Sa.K. and O.L. All authors have read and agreed to the published version of the manuscript.

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Van Puyvelde, S., de Block, T., Sridhar, S. et al. A genomic appraisal of invasive Salmonella Typhimurium and associated antibiotic resistance in sub-Saharan Africa. Nat Commun 14, 6392 (2023). https://doi.org/10.1038/s41467-023-41152-6

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Received: 10 June 2022
Accepted: 23 August 2023
Published: 23 October 2023
DOI: https://doi.org/10.1038/s41467-023-41152-6

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