Abstract
Photoswitches are indispensable tools for responsive chemical nanosystems and are used today in almost all areas of the natural sciences. Hemiindigo (HI) derivatives have recently been introduced as potent photoswitches, but their full applicability has been hampered by the limited possibilities of their functionalization and structural modification. Here we report on a short and easy to diversify synthesis yielding diaryl-HIs bearing one additional aromatic residue at the central double bond. The resulting chromophores offer an advantageous property profile combining red-light responsiveness, high thermal bistability, strong isomer accumulations in both switching directions, strong photochromism, tunable acid responsiveness, and acid gating. With this progress, a broader structural realm becomes accessible for HI photoswitches, which can now be synthetically tailored for advanced future applications, e.g., in research on molecular machines and switches, in studies of photoisomerization mechanisms, or in the generation of smart and addressable materials. To showcase the potential of these distinct light-responsive molecular tools, we demonstrate four-state switching, chemical fueling, and reversible inscription into transparent polymers using green and red light as well as acid/base stimuli, in addition to a comprehensive photochemical study of all compounds.
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Introduction
Molecular photoswitches have occupied a prominent place in all chemistry-related fields as they allow facile introduction of responsiveness at the nanoscale1,2. The field of photoswitch research has developed rapidly especially over the past decade. In its wake the utility of photoswitches has expanded to vastly different areas of applications bringing increasingly complex phenomena under the control of light-signaling in a truly bottom-up fashion. With the aid of photoswitches it has become possible to remote-control bioactivity of drug-like molecules3,4,5,6, change properties of materials7,8,9,10,11, steer catalytic selectivity12,13, influence supramolecular assemblies14,15,16, or move molecular machines17,18,19,20. Such steep progress showcases the potential of this diverse class of molecular enablers. It is inevitably tied to advances at the fundamental level and consequentially unknown molecular photoswitch classes21, modes of geometry changes, modes of electronic alterations, and types of photoreactions are being actively researched. Recent examples are modified azobenzene22,23,24,25,26,27,28,29, hydrazone-30, Stenhouse dye-, imidazolyl-radical-31, or indigoid photoswitches32,33,34,35,36,37, each of which offering their own specific advantages. Our group has investigated indigoid chromophores intensively and transformed many of long known dyes in this class into capable photoswitching motives. A distinct advantage is the responsiveness of indigoid core-chromophores to visible light, so that no damaging UV-irradiation is needed for their operation. Additionally, geometries are typically rigid with only limited degrees of freedom facilitating precise control over shapes and spatial relations of functional groups upon switching. Hemithioindigo (HTI) is a prominent representative38, consisting of a thioindigo fragment and a stilbene fragment fused together via a photoisomerizable double bond (Fig. 1a). Distinct advances in applicability and photoswitching performance of HTI were made when a fourth substituent could be introduced at the double bond (termed R1 in Fig. 1a)39,40. With this substitution pattern it became possible to directly evidence a long proposed basic photoreaction, the hula twist41, discover a previously unknown photoreaction, the dual single bond rotation (DSBR)42, devise fundamentally different types of molecular motors with distinct modes of motion and working mechanisms43,44, build advanced multi-state photoswitches34,42, or establish the first light-energy powered molecular gearing system45.
The related HI structure features an indigo-fragment instead of the thioindigo fragment (Fig. 1a) and shows similar high potential46. Monoaryl-HIs bearing strong electron donors at the stilbene fragment represent nearly perfect photoswitches, delivering almost quantitative isomer conversions in both switching directions, red-light responsiveness, high extinction, strong photochromism, sizable quantum yields (QYs), and solvent-independent high thermal bistability36. Nitrogen substitution at the indigo fragment allows facile functionalization resulting in distinct applications e.g. as chiroptical switches with unusual electronic circular dichroism (ECD) responses37 or ionic chromophores for gas phase photoswitching47. Likewise, HI applications in biological context to photomodulate HIV-1 RNA binding48 or photoswitching in the presence of bovine serum albumin49 have been demonstrated already. For these reasons it can be stated that HIs are highly promising photoswitches, yet their full potential awaits to be explored. Currently different structural modifications of monoaryl-HIs are investigated to improve property design and applicability50,51,52,53. In light of the advances made with HTIs, diaryl-substitution would be a fundamentally important next step to significantly elevate the capacity of HI photoswitches (Fig. 1b). The resulting structures harbor three (or even four, if the indoxyl benzene ring is also functionalized) different substitution sites, the spatial relations of which are fully controllable during switching. The additional aryl-substitution site of the HI chromophore can then be invoked to tailor favorable electronic and photophysical properties such as photochromism or absorption ever more precisely.
In this work we disseminate the synthetic methodology for generating diaryl-HI chromophores (1a-4c), a comprehensive photophysical and photochemical analysis of their photoswitching properties, and showcase possible applications for multi-state switching and functional materials. We demonstrate how these structures can be generated in a straightforward, easy to diversify, low-step count, and versatile synthesis. We also evidence them as highly capable photoswitches possessing red-light responsiveness, high thermal bistability, strong isomer accumulations in both switching directions, strong photochromism, tunable acid responsiveness, competence for acid gating, and applicability for photoswitching within functional polymers.
Results and discussion
Synthesis
To develop a synthesis for diaryl-HIs, important prerequisites need to be taken into consideration, as the synthesis of diaryl-HTIs could not be adapted to access diaryl-HIs (Dieckmann-type condensations of different anthranilic acids/acid chlorides/esters followed by chlorination with oxalyl chloride were not successful and also condensations of indoxyl derivatives with ketones did not deliver the desired products). First, the parent HIs with one residual H-atom at the central photoisomerizable double bond are easily accessible in high yields by condensation of commercially available aldehydes (introducing R2) and indoxyl acetate (Fig. 2). Therefore, the parent HIs would be ideal starting materials for introducing a fourth substituent at the double bond (R1) as well as nitrogen substituents replacing the NH proton (R3) later. Second, introduction of a fourth substituent should be versatile and thus a common precursor allowing easy diversification by well-established broad-scope methodology was deemed preferable. Third, facile alkylation at the indoxyl nitrogen would allow to introduce functionalities for late-stage covalent connection of the HI photoswitches. For these reasons, chlorination of the double bond was chosen to enable subsequent chlorine-substitution reactions with a nucleophile (attempts for Heck reactions were equally unsuccessful as attempts of directly generating metal organic species by C–H activation at position R1). The envisioned chlorination was inspired by the described substitution at the Michael system double bond (R1) with a nucleophilic reactant such as KCN and subsequent oxidation54 (note: attempts at subsequent cross coupling reactions of HIs with nitriles at position R1 were not successful). However, employing related reactants such as KCl or KBr instead of KCN did not lead to halogenated product formation. Changing the electronic character of the reactant from a nucleophilic halide to an electrophilic halonium ion was then attempted for substituting at the desired position R1. However, this electrophilic reaction faces selectivity problems as either the para or ortho position to the nitrogen at the indoxyl benzene are nucleophilic. It was thus found rather expectedly that halogenation with N-bromosuccinimide (NBS) leads to dominant substitution of the electrophilic para position at the indoxyl benzene ring, similar to the literature known bromination of isatin55. Attempts at chemoselective bromination reactions at the β-position of the Michael system56, as well as bromination with elemental bromine also did not lead to successful functionalization at position R1. With N-chlorosuccinimide (NCS) the R1 position was chemoselectively accessible without side reactions at the indoxyl benzene. Because of the inherent Michael system within the HI structure, exchange of the chloride could then be accomplished by a direct nucleophilic substitution, using cross coupling chemistry, or even radical chemistry if needed. A final introduction of an ester function at the indoxyl nitrogen atom (R3) was deemed suitable as a versatile handle for facile implementation of HI photoswitches, e.g., in peptides, natural products, or polymers. These considerations led to the modular synthesis shown in Fig. 2 (details about methods, synthesis, and characterization are outlined in Supplementary Notes 1–3, Supplementary Table 1, and Supplementary Figs. 1–34).
The particular Rx-groups at the diaryl-moiety were chosen first and foremost to facilitate the photoswitching properties. For proper photochromism and switchability one electron-rich aromatic residue is needed, similar to monoaryl-HIs36,37,47,51, HTIs57,58,59,60,61, and the recently reported diaryl-HTIs40,62. Thus a p-aniline (p-NMe2) residue was combined with a second neutral or electron-poor residue at the diaryl-moiety, which needs to be different in its electronic nature in order to distinguish E and Z isomers from each other.
As described previously, the condensation reaction between indoxyl acetate and different aldehydes leads to very high yields of the resulting parent monoaryl-HIs 5 to 8. Chlorination of parent HIs also proceeded in good to very good yields when using NCS typically at 23 °C in tetrahydrofuran (THF), with the notable exception of the electron rich HI 7 (R2 = p-NMe2). In this case substantial chlorination at the α position to the p-NMe2 is observed as side reaction. In general, aprotic solvents reduce side reactions at the indoxyl benzene, and high concentrations of the HI further improves the yield. The subsequent Suzuki-Miyaura cross coupling reactions with aromatic boronic acids delivered the corresponding diaryl-HIs in moderate to high yields. A final N-alkylation of the indoxyl nitrogen (R3) using either 1-iodopropane or methyl acrylate delivered the highly functionalized HI photoswitches 1b-4c. Crystals suitable for X-ray analysis were obtained for HIs 1a and 3b evidencing the twisted nature of the diaryl moieties as well as the constitution of the photoswitches (Fig. 2c, d, Supplementary Note 10, and Supplementary Table 12).
Thermal behavior
To establish the switching properties of diaryl-HIs 1a-4c, their thermal behavior in the dark was scrutinized first (Table 1, Supplementary Note 7, Supplementary Table 5, Supplementary Figs. 93–104). In most cases, slow isomerization takes place at elevated temperatures to establish an equilibrium with both Z and E isomers being present in significant amounts. This behavior also allows to directly measure the relative free enthalpy differences ΔG between isomers as summarized in Table 1. When analyzing the kinetics of thermal E/Z or Z/E isomerizations in the dark, the corresponding Gibbs energies of activation ΔG‡ could be obtained. All HIs exhibit high thermal bistability as manifested in sizable ΔG‡ values, which are >25 kcal mol-1 giving rise to corresponding half-live times of isomers ranging from hours to >100 years at 25 °C (linearly extrapolated from elevated temperature measurements). The only exceptions are HIs 2b and 3c, which are thermally more labile with corresponding half-lives in the minute to hour range, respectively. It is further observed that increasing steric hindrance at the non-p-NMe2 aryl residue of the diaryl-moiety increases the thermodynamic stability of the bathochromic isomer.
Photoswitching properties
With high bistability being established, the photophysical and photochromic properties of HIs 1-4 were scrutinized next (see Table 1 and Fig. 3). All compounds show well-separated UV/Vis absorption spectra for their E and Z isomers (Fig. 3, Supplementary Note 4, Supplementary Table 2, and Supplementary Figs. 35–44). In general, isomers in which the carbonyl group of the indigo fragment and the p-NMe2 substituent are residing at the same side of the double bond possess bathochromically shifted absorptions. They are referred to as bathochromic species in the following, and the corresponding other isomer as hypsochromic species (see “Methods: Synthesis” section). This behavior gives rise to pronounced photochromism with maxima differences reaching 40 nm (Supplementary Note 5: Supplementary Table 3, and Supplementary Figs. 46, 48–50, 52, 53, 55, 59–63, 65, 67, 68, 72–74, and 78–80). Associated with this very good absorption separation is a significant color contrast between the two isomers in solution as shown in Fig. 3 (see Supplementary Note 5 for the corresponding photographs of all derivatives in Supplementary Figs. 46, 48, 52, 55, 59, 63, 65, 67, 72, and 78). Compared to structurally related monoaryl-HIs36 or diaryl-HTIs40,62, the here presented diaryl-HIs show strongly bathochromic shifted UV/Vis absorbance bands (up to 30 nm shifted compared to the most bathochromic shifted structurally similar derivatives available so far). Also in comparison to pyrrole heterocycles fused to HTIs63 or electron rich monoaryl-HTIs64, the here presented systems exhibit more bathochromically shifted UV/Vis absorbance bands and similar very good band separation of the corresponding isomers. The comparison of UV/Vis spectra measured in solvents of different polarity show, that the absorbance maxima of the hypsochromic isomers undergo a further bathochromic shift in polar and highly polar solvents such as THF, methanol (MeOH), dimethylsulfoxide (DMSO) or DMSO/H2O, whereas the bathochromic species exhibits hypsochromic or bathochromic shifts (Supplementary Note 5, Supplementary Table 4, and Supplementary Figs. 49, 50, 58, 60-62, 71, 73, 74, 77–80). The molar absorption coefficients of diaryl-HIs are in the range of 4000–15,300 L mol−1 cm−1 and thus are perfectly suited for photoswitch applications (Table 1). The noticeable decrease in molar absorption coefficients in comparison to monoaryl-HIs36 is owed to significantly twisted aromatic substituents as a result of their mutual steric hindrance (Figs. 2 and 3, Supplementary Note 4, Supplementary Table 2, and Supplementary Figs. 35–44, corresponding crystal structures are described in Supplementary Note 10). A similar influence of molecular twisting on the molar absorption coefficients has been described, i.e., for HTI photoswitches before60,63. Photoisomerization reactions of the highly substituted HIs were quantified by UV/Vis and 1H NMR spectroscopy to cover a range of concentrations and test the robustness of photoswitching (Supplementary Note 5, Supplementary Tables 3 and 4). Photoisomerization reactions that led to enrichment of the bathochromic species resulted in nearly quantitative isomer enrichment in all cases except for HIs 3a and 3c for which 53% and 72% of the bathochromic species are obtained, respectively. Irradiation with light of longer wavelengths resulted in enrichment of the hypsochromic species with more variation in individual isomer yields (46% - 93%). The individual NMR experiments are shown in Supplementary Figs. 45, 47, 51, 54, 56-58, 64, 66, 69–71, and 75–77). Increasing polarity of the solvent does not hamper photoswitching abilities but slowed down photoconversion is observed in DMSO solution (Supplementary Figs. 50 and 62). Thermal stability is reduced in protic solvents for most diaryl-HIs except for 1b and 1c (Supplementary Figs. 49, 50, 61, 74, and 80). QYs were found to be below 1% for photoisomerization of bathochromic species to hypsochromic species of diaryl-HIs. The QYs for photoisomerization of hypsochromic species to bathochromic species were determined to be between 4% to 6% (2b, 2c, 4b, and 4c). Individual data are presented in Supplementary Note 6, Supplementary Table 5, and Supplementary Figs. 81–92).
Taken together diaryl-HIs 2c, 4b, and 4c possess the ideal combination of high thermal bistability, bathochromically shifted absorptions, strong photochromism, strong enrichment of each isomer in the respective pss, red and blue light responsiveness, highest QYs in the series, and high level of functionalization. However, also derivatives 1b, 1c, and 3b deliver with quantitative isomer conversion at blue light irradiation and beyond 60% conversion with yellow and red-light irradiation. Derivatives 1b and 1c are interesting in their own right as they are highly bistable with half-lives in the dark beyond 100 years at 25 °C (linearly extrapolated from high temperature measurements) and are significantly resistant to acid-induced isomerization. They also appear thermally highly bistable in protic solvents such as MeOH and DMSO/H2O mixtures while retaining their photoswitching capacity. Looking at the photophysical and photochemical properties of diaryl-HIs 1-4, the following tendencies can be observed. The stronger the donor/acceptor system at the stilbene moiety is, the more bathochromically shifted the UV/Vis absorption bands, the better the photochromism and the better the isomer enrichment in both switching directions are. The substitution of the proton at position R3 improves the photochemical behavior in terms of isomer content in both switching directions in the pss and photochemical stability during irradiation. There is also a tendency, that thermal bistability increases if position R3 is functionalized with alkyl substituents. Substituents at position R3 possessing a stronger –I effect exhibit slightly better thermal bistabilities than those possessing mostly a +I effect. This is interesting as the thermal bistability ΔG‡Z→E of HI 1 can be improved from 25.3 kcal mol-1 (R3 = H) to 31.9 kcal mol-1 (R3 = (CH2)2C(O)OCH3) by making use of inductive effects. Taken the results together, it is evident that a push-pull system cross-conjugated at the central double bond in combination with the substitution of the proton at the indoxyl nitrogen are most effective for high isomer enrichment in both photoswitching directions. However, also a combination of strong and weak electron donated aromatic substituents results in proper photoswitching behavior.
Application for photochromic polymers
The pronounced color contrasts between the E and Z isomers of diaryl-HIs, their high thermal isomerization barriers and moderate QYs are advantageous for applications in functional materials, which are stable under ambient conditions and even under exposure to indirect sunlight. To showcase the versatility of diaryl-HIs and translation of their green/red light responsiveness to the solid phase we have implemented derivatives 1b and 4b into polystyrene (PS) (for polymer application of 4b see Fig. 4 and for 1b and 4b see Supplementary Note 9, and Supplementary Figs. 146 and 147). During thermal polymerization of a mixture of PS and diaryl-HIs, the latter are stable and retain their photoswitching properties (Fig. 4a). The resulting polymer is highly transparent and displays reversible photochromic properties changing from red to deep purple color upon irradiation with red or green light, respectively (Fig. 4b–d). Because of the significant photochromism of diaryl-HIs and their multi-stimuli response (see acid-base induced switching below), it is possible to draw or write with light persistently into the PS polymer (Fig. 4b and c), erase the written inscription with light of another wavelength (Fig. 4d), and also erase by adding acid to the polymer (Fig. 4e). The written areas remain stable within the polymer until several hours under exposure to indirect sunlight (Fig. 4f) and at least for several days to weeks in the dark at 23 °C.
Acid-base induced switching
Besides photoisomerization it is also possible to isomerize the neutral metastable species of diaryl-HIs to the most stable ones by adding acid (Figs. 5a and 6a, Supplementary Note 8, Supplementary Figs. 105, 106, 113, 116, 118, 119, 122, 124–126, and 144, and Supplementary Table 7). Acid-induced isomerization is a function of acid concentration and the intrinsic electronic properties of the respective diarly-HI. The more pronounced the cross conjugation at the diaryl-fragment, i.e., by donor-acceptor (R1/R2) substitutions, the faster is the acid-induced isomerization. Fast acid-induced isomerization is also seen in cases where R3 = H. However, in the absence of a donor-acceptor system at the diaryl-fragment, acid-induced isomerization proceeds much slower and significant amounts of acid are needed to accelerate it (e.g., in the case of 1b and 1c, Supplementary Figs. 107, 110, and 112, and Supplementary Table 7). Therefore, a pH sensitive switching can be established for diaryl-HIs where acid addition serves as another stimulus that leads to facile thermal isomerization (Fig. 5a). Further, addition of excess of acid enables access of a third and also a fourth state of diaryl-HIs (Figs. 5b and 6, Supplementary Figs. 107, 108, 111, 114, 115, 120, and 121). The third state represents the protonated form of the corresponding thermodynamically more stable neutral isomer, and the fourth state represents the protonated form of the thermodynamically less stable neutral isomer (inversion of thermodynamic stabilities can be observed between neutral and protonated forms as described below).
To illustrate the multi-switching properties, derivative 4b is discussed in more detail in the following. After adding small amounts of TFA (up to 4.0 equiv.) to a toluene solution of enriched bathochromic E-4b, the UV/Vis spectrum does not change significantly at first. However, over the course of 25 min at 23 °C the absorption spectrum transforms into the known absorption spectrum of the thermodynamically more stable hypsochromic Z-4b isomer (Fig. 5a, Supplementary Figs. 124–126, and Supplementary Table 7). In the presence of such small amount of TFA a different reversible switching mode can thus be established, in which 505 nm light leads to photoisomerization from Z-4b to E-4b and the reverse isomerization proceeds thermally activated in the dark (Fig. 5a). Multiple switching cycles in the presence of 0.25 equiv. TFA and 505 nm light are possible (Supplementary Figs. 124 and 125). However, reversible photoswitching is still possible using 625 nm light for photoisomerization from E-4b to Z-4b (Supplementary Fig. 127). When adding a base like Et3N, high thermal stability is recovered changing the switching mode to sole reversible photoisomerizations using 505 nm and 625 nm light (Fig. 5c and Supplementary Figs. 131 and 132). The acid-induced and accelerated isomerization to hypsochromic Z-4b has also been employed in the polymer-application of this diaryl-HI to effectively erase inscribed information from the sample (Fig. 4e).
Upon addition of a large excess of TFA (up to 5000 equiv.) to bathochromic E-4b or hypsochromic Z-4b distinct changes in the absorption are observed. Most important is the missing band centered at around 420 nm, which gives the solution a distinct and lighter purple color to the human eye (Figs. 5b, c and 6c). Two distinct new absorption spectra can be obtained, which are ascribed to two new states identified as the protonated forms of the E- and Z-isomers, respectively. The third state is assigned to the Z-[4b-H]+ isomer with a hypsochromically shifted absorption and the fourth state to the E-[4b-H]+ isomer with a bathochromically shifted absorption (Figs. 5b and 6). The assignments were made because of the following observations. Mass spectroscopy experiments revealed that no twofold protonated diaryl-HI species were formed even after adding large excess of TFA (Supplementary Tables 8-11). NMR analysis revealed, that in pure TFA-d1 solution only the E-[4b-H]+ species is present (Supplementary Figs. 140–143). An 1H NMR titration experiment in toluene-d8 solution shows that increasing amounts of added TFA-d1 convert the initially 77% of Z-4b (thermodynamically most stable isomer in the neutral form) completely to the protonated E-[4b-H]+ species (after 43 equiv. TFA-d1 added to a 1.12 mm solution of 4b, Fig. 6c and Supplementary Figs. 134–139). When first adding a large excess of TFA (ca. 5000 equiv. at UV/Vis concentrations) to a neutral Z-4b enriched toluene solution and subsequently adding an equally large excess of NEt3 at –78 °C, 91% of the thermodynamically less stable neutral E-4b isomer is formed (see Fig. 6b, Supplementary Fig. 133, and additionally Figs. 117 and 145 for the corresponding experiments with diaryl-HIs 2c and 4c, respectively, Supplementary Table 7). Cooling is important at this point, as locally remaining amounts of acid in the presence of already neutral bathochromic isomers lead to accelerated thermal isomerization to the hypsochromic neutral isomers at ambient temperatures. The sequence of acid and base addition thus establishes a chemical fueling process in which the thermodynamically less stable neutral E-4b isomer can be obtained almost quantitatively.
In a separate experiment, only up to 1000 equiv. of the NEt3 base were added after 4000 equiv. of TFA addition instead of completely neutralizing. In this case a different distinct change of the absorbance is observed, which is mainly manifested in a global hypsochromic shift. This new spectrum was assigned to the Z-[4b-H]+ species because subsequent complete neutralization with NEt3 fully recovers the absorbance spectrum of the thermodynamically more stable neutral Z-4b isomer (Fig. 5b and Supplementary Fig. 130). Therefore, switching between the two protonated states is possible when preparing the solution by addition of excess acid to the neutral species first (up to 4500 equiv. of TFA). When starting from Z-4b, first Z-[4b-H]+ is formed which quickly isomerizes to E-[4b-H]+ (Supplementary Figs. 128 and 129). Subsequent addition of 1000 equiv. of Et3N leads to the isomerization from E-[4b-H]+ to Z-[4b-H]+ (Fig. 5b and Supplementary Fig. 130).
To sum up the conducted experiments, acid-induced switching in both directions between the E-[4b-H]+ and Z-[4b-H]+ isomer, between the E-[4b-H]+ and E-4b isomer, and between the Z-[4b-H]+ and Z-4b isomer is possible. Additionally, acid-induced one directional switching between the Z-4b and E-[4b-H]+ isomer (Supplementary Fig. 131), between the E-4b and Z-[4b-H]+ isomer (Supplementary Fig. 132), and between the E-4b and Z-4b isomer is also possible establishing reversible four-state switching with high isomer enrichment in each case for 4b (Fig. 6a and Supplementary Fig. 123).
For 1b the neutral Z-1b isomer is only slightly more stable than E-1b (thermally equilibrated mixture in o-xylene-d10 of E:Z = 42:58) and cannot be enriched significantly by simple heating experiments. However, the analogous sequential acid/base addition experiment with 1b at 23 °C (adding 5000 equiv. TFA and neutralization with 5000 equiv. NEt3) leads to an absorption spectrum signifying that the Z-1b isomer is populated to 85%. Because of the previously described resistance of diaryl-HIs 1 against acid-induced thermal isomerization (Supplementary Fig. 109) cooling is not necessary in this experiment.
A general trend is found, where protonation of the p-NMe2 substituent inverts (e.g., diaryl-HI 4b) or at least pushes thermodynamic stabilities (e.g., diaryl-HI 1b) towards the bathochromic isomers. This can be explained as follows. In its neutral form the hypsochromic Z-4b isomer is the thermodynamically more stable one, in which the carbonyl group of the indigo fragment and the strongest donor substituent (p-NMe2) are residing at the opposite side of the double bond. Protonation of p-NMe2 converts it into the strongest acceptor and the cyano-substituent (p-CN) into a (relatively) stronger donor substituent. As a result, the protonated bathochromic E-[4b-H]+ conformation becomes the thermodynamically preferred one placing again the strongest donor of the diaryl-moiety and the carbonyl at opposite sides of the double bond.
The two protonated species are thus spectroscopically distinct and represent a third and fourth reversibly accessible state of diaryl-HIs (Figs. 5b, c and 6b). In the protonated forms no photoswitching is possible, establishing acid-gating of photoresponsiveness. Neutralization with Et3N recovers the absorption profile of hypsochromic Z-4b (Fig. 5b) or bathochromic E-4b (chemical fueling resulting in 91% of thermodynamically stable isomer, Fig. 6b) and at the same time the photoswitching capacity (Fig. 5c), which evidences reversibility of acid-gating. The protonated states can also be elicited in all other diaryl-HI derivatives (Supplementary Note 8). However, it should be noted at this point that diaryl-HIs 1b and 1c are much less sensitive towards acid addition and retain their bistable photoswitching abilities even in the presence of significant amounts of TFA. Addition of large excess of TFA followed by neutralization with NEt3 results in 85% of the Z-1b isomer, which establishes chemical fueling also for this diaryl-HI derivative (Supplementary Fig. 109).
In summary, we report on a structurally expanded class of HI chromophores, which are diaryl substituted at the central double bond. Synthetic access to these compounds is straightforward and modular, such that a large variety of substitution pattern can be introduced not least for late-stage functionalization and covalent attachment of the photoswitches. The switching properties were quantified evidencing a suite of favorable properties such as visible and red light responsiveness in both switching directions, significant photochromism, high thermal bistability, strong enrichment of each isomer in the pss, and large yet defined geometry changes upon switching. Acid and base addition can be used as two further independent stimuli establishing a third and fourth spectrally distinct state of diaryl-HIs, which are the protonated Z and E isomers, respectively. Reversible switching and selective strong accumulation of each of the four states over up to three different pathways is possible depending on relative concentrations of acid and base. What is more, this system allows to strongly populate thermodynamically less stable isomers via acid/base additions, a process which can be related to chemically fueling. These multi-switching properties and the associated acid-gating of photoswitching capacity add further response dimensions and functional complexity. Application of diaryl-HIs in transparent and highly colored PS polymers showcases the versatility, multi-stimuli response, and intrinsic advantages, like color contrast and photostability, directly. Building on the results of this study it will now be possible to investigate a large and unexplored structural space as well as multi-stimuli and multi-state applications for this potent class of photoswitches.
Methods
General materials and methods are outlined in Supplementary Note 1.
Synthesis
The synthesis of the parent monoaryl-HIs 5-8 followed published procedures that have been modified in terms of solvents and temperatures used (Supplementary Table 1a)32,36,65. The selective chlorination with NCS at the stilbene part of the parent monoaryl-HIs 9-12 has not been described so far, but was inspired by different electrophilic functionalization reactions of similar compounds (Supplementary Table 1b)54,55,56,66. The Suzuki-Miyaura cross-coupling reactions of chlorinated monoaryl-HIs with boronic acids to yield diaryl-HIs 1a-4a were inspired by the published synthesis of diaryl-HTIs but were modified for the needs of diaryl-HIs (Supplementary Table 1c)40. N-propylated diaryl-HIs 1b-4b were prepared according to the published procedure of C. Petermayer et al. for the N-alkylation of monoaryl diaryl-HIs (Supplementary Table 1d)36. N functionalization with methyl propionate at the indoxyl part to yield diaryl-HIs 1c-4c follows a published Aza-Michael addition (Supplementary Table 1e)67. Details on the syntheses are given in Supplementary Note 2. An overview of the synthesis route, substrate scope and yields is given in Supplementary Table 1. H NMR and 13C NMR spectra of all compounds are given in Supplementary Note 3. 1D 1H-1H NOE NMR spectra and 2D 1H-1H NOESY NMR spectra of compounds 1a-4a, 1b-4b, and 1c-4c allow direct experimental assignments of E and Z isomers. This structural elucidation (Supplementary Note 3) together with the determination of molar absorption coefficients (Supplementary Note 4) establishes that isomers with the carbonyl group of the indoxyl fragment and the p-NMe2 substituent residing at the same side of the double bond possess bathochromically shifted absorptions.
Determination of molar absorption coefficients of pure E and Z isomers
The molar absorption coefficients of pure E and Z isomers were obtained from samples containing E + Z isomer mixtures. First, the UV/Vis absorption spectra of pure E and Z isomers were obtained from the UV/Vis absorption spectra of two solutions with different E + Z mixtures, known isomeric compositions, and known total concentrations. To extract the individual absorption spectra of each isomer in this case, the sample must be composed of only two isomers, which photoisomerize without side reactions or decomposition. Details are given in Supplementary Note 4. A summary of the determined molar absorption coefficients for diaryl-HIs 1-4 are presented in Supplementary Table 2.
Photoisomerization experiments using UV/Vis and NMR spectroscopy
Photoisomerization experiments were conducted with diaryl-HI solutions filled in NMR tubes or in 10 mm Hellma quartz cuvettes, which were irradiated from the outside with LEDs of different wavelengths. Solutions were prepared either with concentrations of 2 × 10−2 to 8 × 10−2 mol L−1 in toluene-d8 or THF-d8 (NMR tubes) or with concentrations of 3 × 10−5 to 8 × 10−5 mol L−1 in regular non-deuterated toluene, MeOH, DMSO or DMSO/H2O (spectroscopic grade, UV/Vis cuvettes). Experimental details and results are presented in Supplementary Note 5. A summary of the isomer compositions of diaryl-HIs 1-4 in the pss at NMR concentration is presented in Supplementary Table 3, the corresponding date obtained from UV/Vis measurements are summarized in Supplementary Table 4.
Quantum yield determination
Determination of QYs for the hypsochromic to bathochromic photoisomerization followed the initial slope method. Therefore, QY determinations were carried out in UV/Vis cuvettes charged with E + Z isomer mixtures diluted to spectroscopic concentrations of 5 × 10−5 to 2 × 10−4 mol L−1 in toluene. Details are outlined in Supplementary Note 6. A summary of measured values is given in Supplementary Table 5.
Thermal stabilities of isomeric states
The thermal E to Z or Z to E isomerizations are described as two unimolecular elemental reactions, i.e., the thermal forward isomerization and the thermal backward isomerization. These can be seen as first-order kinetics with entering thermodynamic equilibria. From kinetic analysis the corresponding Gibbs energies of activation ΔG‡E→Z/ΔG‡Z→E for the thermal equilibrations are obtained. From the isomer composition in thermal equilibrium the Gibbs free energy differences ΔG of the isomers are calculated. Thermal isomerization kinetics and relative isomer stabilies of diaryl-HIs 1-4 were measured at various temperatures in toluene-d8 or o-xylene-d10 solutions. Details are outlined in Supplementary Note 7. A summary of measured values is shown in Supplementary Table 6.
Acid-induced isomerization
Acid-induced isomerization experiments at UV/Vis level were conducted with diaryl-HI solutions filled in 10 mm Hellma quartz cuvettes, which were irradiated from the outside with LEDs of different wavelengths. After addition of different equivalents of TFA the isomerization response (thermal and photochemical) of diaryl HIs 1-4 as well as the response to NEt3 addition was monitored by UV/Vis spectroscopy. Additionally, an acid titration experiment with TFA-d1 (0.1 to 135 equiv.) was performed with a thermally equilibrated Z/E-4b mixture at constant diaryl-HI concentration (1.12 mM) in toluene-d8 at 25 °C using 1H NMR spectroscopy for analysis. Beforehand, the proton signals of the neutral E-isomer and neutral Z-isomer of 4b in toluene-d8 were assigned to the structure and the double bond configuration was elucidated through 1D 1H-1H NOE NMR experiments. The identity of the isomer reached at the end point of the titration was elucidated through a comprehensive NMR analysis in TFA-d1 solution. Additional, MS-APPI and MS-ESI mass experiments in the presence of different amounts of TFA were performed in toluene solution. Comprehensive experimental details are provided in Supplementary Note 8. A brief summary of the experimental results is presented in Supplementary Table 7.
Isomerization within polymers
For each sample, 30 mL of neat styrene were heated to 140 °C for 50 min until polymerization was almost completed. Then, ca. 4 mg of diaryl-HI was added, and the liquid was mixed thoroughly. The liquid diaryl-HI containing polymer was then poured into petri dishes and allowed to cool to 23 °C. After 24 h, a solid and transparent PS polymer was obtained. Samples were irradiated with certain wavelengths to enrich the hypsochromic or bathochromic isomers, respectively. Different methods to erase the written inscriptions (light, acid, sunlight) were tested. Additional experiments are presented in Supplementary Note 9.
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Data availability
Source data are provided with this paper. Crystal Structural Data were deposited in the Cambridge Crystallographic Data Centre with the following CCDC designations: 2191572 and 2191573. All data generated during this study are available from the corresponding author upon request.
Change history
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Acknowledgements
H. Dube thanks the Deutsche Forschungsgemeinschaft (DFG) for an Emmy Noether fellowship (DU 1414/1−2, GEPRIS number 264598191). This project has also received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (PHOTOMECH, grant agreement No 101001794).
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H.D. and M.S. conceived and designed the project. M.S. synthesized and characterized all compounds, crystallized compounds for X-ray analysis, conducted all thermal, photochemical, and acid/base related measurements, data collection, and analysis, and realized the polymer applications. F.H. determined the structures in the crystalline state. H.D. and M.S. drafted the paper, H.D. edited the Supplementary Information and M.S. compiled the Supplementary Information. All contributors discussed, edited, and refined the paper.
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Sacherer, M., Hampel, F. & Dube, H. Diaryl-hemiindigos as visible light, pH, and heat responsive four-state switches and application in photochromic transparent polymers. Nat Commun 14, 4382 (2023). https://doi.org/10.1038/s41467-023-39944-x
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DOI: https://doi.org/10.1038/s41467-023-39944-x
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