Advances in farming technology and intensification of animal agriculture increase the cost-efficiency and production volume of meat. Thus, in developed nations, meat is relatively inexpensive and accessible. While beneficial for consumer satisfaction, intensive meat production inflicts negative externalities on public health, the environment and animal welfare. In response, groups within academia and industry are working to improve the sensory characteristics of plant-based meat and pursuing nascent approaches through cellular agriculture methodology (i.e., cell-based meat). Here we detail the benefits and challenges of plant-based and cell-based meat alternatives with regard to production efficiency, product characteristics and impact categories.
Global production and consumption of meat continue to surge as demand is driven upward by population growth, individual economic gain, and urbanization1,2. In 2012, the Food and Agriculture Organization (FAO) of the United Nations projected the global demand for meat would reach 455 M metric tons by 2050 (a 76% increase from 2005)3. Likewise, the global demand for fish is projected to reach 140 M metric tons by 20504. The majority of this incline is attributed to middle-income countries (e.g., China), as consumption in higher-income countries is relatively stagnant or marginally decreasing (e.g., United Kingdom) and in lower-income countries, the rate of consumption is fairly constant (e.g., India)1. This pattern is consistent with a proposed theory that the relationship between meat consumption and income follows an “inverted U-shaped” trend; consumption initially increases with rises in income but eventually reaches a turning point at which consumption stagnates or declines5. This observation may be rationalized by correlations between high income and increased concern for the consequences of animal agriculture5.
This rising demand is problematic as current methods of large-scale animal husbandry are linked to public health complications, environmental degradation and animal welfare concerns. With regard to human health, the animal agriculture industry is interconnected with foodborne illness, diet-related disease, antibiotic resistance, and infectious disease6,7. Notably, zoonotic diseases (e.g., Nipah virus, influenza A) are linked to agricultural intensification and meatpacking plants in the United States were hotspots for COVID-19 outbreaks7,8. Animal agriculture also contributes to environmental issues including greenhouse gas emissions, land use, and water use1. The United Nations Intergovernmental Panel on Climate Change released a 2018 report asserting that greenhouse gas emissions must be reduced 45% by 2030 to prevent global temperatures from increasing 1.5 °C; a target that could mitigate catastrophes associated with a 2.0 °C increase9. Conventional mitigation techniques include improvements in reforestation, soil conservation, waste management as well as tax policy, subsidies, and zoning regulations10. While these strategies remain important, the urgency of climate change may require more transformative approaches. Lastly, with regard to animal welfare concerns, each year billions of animals are killed or suffer either directly (e.g., farm animal slaughter, seafood fishing) or indirectly (e.g., fishing by-catch, wildlife decline due to habitat destruction) in relation to human food systems11,12.
The majority of the aforementioned issues can be attributed to the fact that the raw material inputs (i.e., animals) for conventional meat production are inherently unsanitary, inefficient, and sentient. By removing animals from the manufacturing process, several externalities may be alleviated. Plant-based meat (PBM) and cell-based meat (CBM) approaches offer to generate food from non-animal sources. While traditional PBMs (e.g., tofu) have existed for centuries, novel PBM alternatives with enhanced sensory characteristics have been commercialized more recently (Fig. 1)13. Other groups have initiated the development of a new field—cellular agriculture. Cellular agriculture describes the tactic of producing commodities from cells, rather than whole organisms or animals, such as CBM; meat grown from muscle or fat cells rather than cows, pigs or chickens. While there is evidence to support certain benefits of these approaches compared to animal-based meat (ABM), it is important to more comprehensively evaluate impacts on human health and the environment as production systems evolve. In addition, widespread adoption of these products will require more direct benefits to the consumer; such as taste, cost, and convenience14. This review serves to compare plant-based (i.e., meat analogs composed of plant proteins) and cell-based (i.e., meat analogs generated from cell cultures) methods to educate stakeholders about the strengths and challenges of each approach and highlight areas of uncertainty.
History and approach
Plant- and fungi-based meat (PBM) products encompass the flavor, texture, and/or nutritional aspects of meat but are different in composition; namely are made from non-animal sourced materials. Based on the time of development and technical complexity, PBM products can be differentiated into two flexible categories: traditional and novel (i.e., next-generation)13. Traditional meat analogs were developed thousands of years ago in Asia and include relatively simple derivatives from soybeans (i.e., tofu, tempeh) or wheat (i.e., seitan)13. In contrast, novel PBMs are characterized by the design and marketing of products as near equivalent replacements for ABM with regard to taste, texture, and nutrition. Product categories can also exist between traditional and novel, as they may meet some but not all of the aforementioned criteria. A distribution map of global companies and brands developing novel PBMs can be found in Fig. 2. Typically, the production of PBM includes three steps15: (i) Protein isolation and functionalization—Target plant proteins are extracted from plants, some of which are subjected to hydrolysis in order to improve their functionalities such as solubility and cross-linking capacity; (ii) Formulation—The plant proteins are mixed with ingredients to develop meat texture such as food adhesives, plant-based fat and flour. Nutrients are added to match or exceed the nutrient profile of the meat. (iii) Processing—The mixture of plant proteins and other ingredients undergo protein reshaping processes (e.g., stretching, kneading, trimming, pressing, folding, extrusion, etc.) to form a meat-like texture. Innovative technologies being utilized to improve the organoleptic properties of PBMs include shear cell technology, mycelium cultivation, 3D printing, and recombinant protein additives16,17.
CBM, also referred to as in vitro meat, lab-grown meat or cultured meat, is meat produced by cultivating cells as opposed to farming animals. CBM technology is based on advances in stem cell biology (e.g., induced pluripotent stem cells) and tissue engineering (e.g., in vitro skeletal muscle grafts) originally purposed for medical applications. CBM production involves four central components: (1) muscle and fat cell isolation and culture, (2) xeno-free culture medium formulation, (3) scaffold development, and (4) bioreactor design; the details of which are described extensively elsewhere14. Interestingly, the concept of CBM can be traced back to 1930 when Frederick Smith, the British Secretary for India, envisioned the genesis of “self-reproducing steaks” through an excerpt of his essay collection The World in 2030 AD, which reads: “It will no longer be necessary to go to the extravagant length of rearing a bullock in order to eat its steak. From one ‘parent’ steam of choice tenderness, it will be possible to grow as large and as juicy a steak as can be desired18.” While CBM has yet to be commercialized in 2020, noteworthy progress has taken place over the past couple of decades. Key milestones include the first CBM patent filed by Willem van Eelen in 199919, the first peer-reviewed research on cultured fish funded by NASA in 200220 and the first cultured beef burger debuted by Maastricht University in 2013. Today, there are dozens of start-up companies around the globe working to bring CBM products to market.
Key sources of protein inputs for novel PBM are relatively inexpensive. The majority of plant-based products are primarily formulated with pea, soy or wheat protein. The agricultural prices (received by farmers in the United States) for these key proteins are 3.8–12.7 times lower than prices received for cattle, hogs, and broilers. In fact, sometimes soy and wheat are combined with ABM to reduce costs for processed meat products21. When standardized by cost (2009 data) per gram of protein, soybeans ($0.01/g) and wheat ($0.03) are still remarkably less costly than cows ($0.32/g), pigs ($0.22/g), and chickens ($0.12/g)22. Despite this glaring discrepancy on the key protein input level, novel PBMs tend to cost more than their animal-based counterparts in a retail setting. This discrepancy may be partially due to processing costs as 94.3% of retail costs for crop products are associated with post-harvest processes, while this accounts for ~50% of the retail costs for beef22. Furthermore, aside from primary proteins, PBMs often include plant-based fats, flavor enhancers, and color additives which contribute to cost. Some consumers are willing to pay premiums for meat substitutes linked to personal health benefits23.
Economic feasibility is a significant hindrance to CBM commercialization24. The cultured beef burger cultivated by Maastricht University in 2013 is reported to have cost $280,400 ($2,470,000/kg) to produce. The production process involved three researchers using bench-scale techniques to culture 20,000 muscle fibers over three months and served as a proof-of-concept rather than an attempt to scale production. A few groups have performed preliminary economic analyses to project the cost of CBM for large-scale production scenarios (Table 1). In 2008, The In Vitro Meat Consortium estimated, by modeling capital and growth medium costs based on data for single-cell protein production, CBM could cost approximately twice as much as chicken25. In 2014, a study speculating on the technical, societal, and economic factors of village-scale CBM production calculated a cost range of $11–520/kg dependent on the price of growth medium24. Invertebrate (e.g., insect, crustacean) cell culture may present a more cost-efficient platform for CBM production based on the unique properties of insect cells (e.g., xeno-free growth medium, high-density suspension culture)26,27. Select companies are targeting high-value products (e.g., foie gras, bluefin tuna, kangaroo meat) in order to lower the bar for reaching price parity. Interesting, a recent consumer acceptance study from the Netherlands reported 58% of participants were willing to pay a 37% premium for cell-based beef compared to conventional animal-based beef28.
PBMs are regulated in a similar manner as other non-animal foods. In the United States, the Food and Drug Administration (FDA), and specifically the Center for Food Safety and Applied Nutrition (CFSAN), oversees food inspection, labeling, packaging, imports, and facility safety. Most PBM products contain simple ingredients that have previously been approved for human consumption. Novel ingredients may be subject to additional evaluation processes. For example, soy leghemoglobin, produced via genetic engineering, filed for “generally recognized as safe” status with the FDA for use as a color additive17. In the European Union (EU), current policy and regulation are supportive for alternative proteins innovation and investment. In 2018, the European Commission presented a “EU Protein Plan”, which encourages the production of alternative proteins for human consumption, and listed existing EU policy instruments that “provide options for strengthening the development of EU-grown plant proteins”. Many novel PBM products are classified under the Novel Food Regulation which regulates “food that had not been consumed or do not exist in the EU before 15 May 1997”23. Australia, Canada, and New Zealand have also introduced legislation to guide oversight of novel foods13. Government oversight is also required for food labeling. In 2018, The United States Cattlemen’s Association petitioned the Food Safety and Inspection Service (FSIS) “to exclude products not derived directly from animals raised and slaughtered from the definition of “beef” and “meat”29. The use of terms such as steak, sausage, bacon, fillet, etc. for PBMs is subject to scrutiny and restriction in many EU member states as well.
Oversight for CBM involves the regulation and monitoring of production, packaging, labeling, and marketing. In the United States, CBM will be jointly regulated by the FDA and the United States Department of Agriculture (USDA) based on a decision announced by the departments in 201930. The FDA will regulate cell isolation, storage, growth, and maturation. After cells and tissues have been harvested, the USDA will monitor products through the remainder of the commercialization process and oversee labeling30. Scaffold materials may fall under FDA food additive provisions31. Even with a joint effort, it will be important to utilize current systems but also to implement new regulation procedures as the technology continues to advance32,33. Further complication could arise if companies intend to sell products containing genetically modified (GM) cells. While the USDA regulates GM crops, a FDA New Animal Drugs Application provision views DNA manipulation to fall under the definition of a drug and dictates FDA oversight of GM animals; this could potentially be interpreted to also apply to GM cells33. A second concern about regulations is with respect to accurate labeling. Similar to the PBM labeling debate, there is an effort to prevent cell-based products from being labeled as “meat”29. Based on the Federal Meat Inspection Act which refers to meat as “any product… made wholly or in part from any meat or portion of the carcass”, there may be justification for CBM to retain its wording. In fact, the North American Meat Institute states that cell-based products likely fall into the definitions of either “meat” or “meat byproduct”34. For Europe, CBM could be applicable to the European Union Novel Food Regulation pathway. While the Food Safety Authority has approved GM food production, contingent on thorough safety assessments, many European countries (e.g., France, Germany, Greece) have banned the production and sale of GM foods35.
The chief organoleptic (i.e., sensory) properties of meat are appearance, aroma, flavor (Table 2), and texture. Novel PBMs mimic the look of ABM by manipulating color, fat marbling and structure (Fig. 3). Depending on the product, PBMs aim to emulate the appearance of raw (e.g., ground meat) or pre-cooked meat (e.g., deli slices). Heat-stable fruit and vegetable extracts (e.g., apple extract, beet juice) or recombinant heme proteins (e.g., LegH) are used to both recreate the color of fresh meat and change to brown upon cooking17,36. To mimic the appearance of fat, some novel PBM products exhibit visible semi-solid plant-based fats (e.g., coconut oil, cocoa butter). Engineering flavor and aroma profiles are important to recapitulate the taste and smell of meat. In meat analogs, flavor additives are incorporated to add, enhance or mask specific flavor notes and generally compose 3–10% of the product21. Many plant proteins are associated with bitter and astringent tastes, which require selective compound removal by post-processing15. In particular, soy products have strong grassy, beany and bitter flavors linked to lipoxygenase, saponin and isoflavone compounds which can be reduced through germination or heating15. A synthetic meat flavor developed in the 1980s was composed of sugar, amino acids, nucleotides, glycoprotein, monosodium glutamate, salt, and fat and determined by a sensory panel to be equal or superior to meat extract37. Recombinant protein additives like LegH can contribute to the flavor as well as the color of PBMs17. PBM texture can be influenced by high-moisture extrusion, shear cell technology, mycelium cultivation and 3D printing. Extrusion, shear cell technology and 3D printing rely on applying mechanical, thermal and shear stresses to a protein mixture to obtain a semi-solid fibrous structure16. While many strategies are available to engineer and tune the texture of plant proteins, it can be difficult to balance processing methods to achieve desired mechanical properties while also retaining nutritional value15. Conversely, mycelium cultivation involves growing filamentous fungi; particular strains of which emulate the microstructure of meat38. Quorn™ is a fungal-based PBM that has provided alternatives for chicken nuggets, meatballs, and minced meat since the 1960s38. New start-ups (e.g., AtLast Food Co., Emergy Foods) are growing mycelia with goals of generating higher quality cuts of meat, such as steak.
To increase the likelihood of mainstream consumer adoption, CBM must be equivalent or superior to ABM from a sensory perspective39. The 2013 cultured beef burger (which contained cultured skeletal muscle tissue but not adipose tissue and was flavored with beet juice, bread crumbs, caramel, egg powder, salt and saffron) was described as tasting “like a real burger” by one panelist and “close to meat, but not that juicy” by another40. Since this milestone, more effort has been focused on generating cell-based adipose (i.e., fat) tissue; given its significant contribution to taste and texture. Advances in engineering fat tissue for use in food have been reviewed in depth elsewhere41. Aside from skeletal muscle and adipose tissue, ABM also contains connective tissue, vasculature networks, and supporting cell types (e.g., fibroblasts). The discrepancies in complexity may result in nuanced flavor differences between ABM and CBM. CBM experts indicate that key flavor profiles can be achieved by co-cultures, medium supplementation, and/or genetic modification14,39. For instance, researchers have explored the effects of supplementing CBM with extracellular heme proteins (e.g., myoglobin)42. Myoglobin is associated with the “bloody” flavor of meat and supplementation was observed to improve the color of CBM constructs without impeding muscle cell growth rates42. Early cell-based prototypes emulate processed meat (e.g., burgers, sausages, nuggets) as it is more difficult to emulate the appearance and texture of whole cuts of meat (e.g., steak). As researchers in the field begin to focus on textural properties, significant effort will be required to evaluate myriad factors (e.g., cell to scaffold ratio, the impact of cooking, packaging, storage, and shipping) on tissue structure. CBM texture can be influenced both by cultured cells and supportive scaffolding materials. In vitro skeletal muscle tissue can be engineered to emulate the structure of meat by employing differentiation and cell alignment strategies. For example, mechanical tension, electrical stimulation, and/or micropatterned substrates can be employed to induce cell alignment in vitro43. A recent study focused on CBM composed of bovine cells coupled with a textured soy protein scaffold; finding some of the samples exhibited texture (i.e., ultimate tensile strength) properties similar to those of native bovine muscle44. In addition, a sensory panel tasted the CBM samples and described “a pleasant meaty flavor” and “a typical meat bite and texture”44.
The key plant-based proteins utilized in PBM formulations (e.g., pea, soy, wheat) provide total protein content at levels on par with ABM. However, in order to ensure a balanced amino acid profile, complementation of multiple plant-based proteins is generally necessary. For instance, legume (low in sulfur-containing amino acids, high in lysine) and cereal (low in lysine, high in sulfur-containing amino acids) proteins are favorable complements. Factors that have been identified in plant proteins that may decrease nutrient bioavailability post-ingestion include: structures resistant to proteolysis, protein conformation, and antinutrients (e.g., tannins, phytates, lectins)45. Certain processing techniques (e.g., soaking, heating, sprouting) have been shown to increase digestibility15. Nutrition is also variable between traditional and novel PBM products. For example, tofu (traditional PBM) and Impossible™ (novel PBM) share certain benefits over ABMs such as containing dietary fiber and minerals while lacking cholesterol. However, tofu-specific benefits include fewer calories, less fat and sodium-free and Impossible™-specific benefits include higher protein and vitamin B12 content (Fig. 4). Concern has been expressed regarding the inclusion of LegH in PBM, citing correlations between heme iron intake and increased risk of diabetes46.
Comprehensive, baseline nutrition data for CBM is not publicly available. Using small sample sizes, the nutrient content of cell cultures can be quantified via laboratory assays27. Different cell types contribute different sets of nutrients; differentiated muscle cells will likely be the primary source of protein and mature adipocytes can contribute to the fatty acid profile41. Certain compounds that are provided by ABM are not present in cultured cells. For example, vitamin B12 is only synthesized by bacteria and will need to be supplemented47. As with flavor, proponents of CBM often claim its nutrition profile will be comparable with or superior to ABM and that nutrients can be tuned via co-cultures, media supplementation, and genetic modification14. Media formulation will have a large impact on the viability and efficiency of the cultured cells, on the nutrition profile, and perhaps also impact flavor and taste. Genetic modification for nutritional improvement is another approach that may be more efficient in the long-term, although genetic approaches may pose problems for regulatory strategy and consumer acceptance. Genetic engineering has already been implemented in livestock to improve various aspects of meat production. In 2004, transgenic swine were generated to express a gene originating in spinach with a goal of improving the fatty acid profile of pork48. This and other modifications could be implemented on a cellular level to influence the properties of CBM. Comprehensive nutrition data for CBM should become available with the launch of initial products, scale-up, and additional interest from the scientific community.
Consumer acceptance is of particular interest to PBM stakeholders who are looking to increase market share. A high consumer acceptance for PBM products was recorded in China (95.6%) and India (94.5%), compared to the United States (74.7%)49. In a European study, the main barriers for dietary inclusion of PBMs were lack of familiarity and low “sensory attractiveness”, and consumers who were unfamiliar with analog products were more likely to want these products to closely imitate ABM50. In a focus group study, motivating factors for not eating ABM ranked differently in Germany (e.g., animal welfare, health, environmental impacts), the Netherlands (e.g., animal welfare, poor meat quality, health), and France (e.g., health, animal welfare, sustainability)51. For all three nations, the taste was the key factor inhibiting consumption of plant proteins, with other factors including habit, convenience and price. In a sensory panel study comparing animal-, plant- and insect-based burgers, animal burgers were associated with the emotional terms of ‘contented, happy and pleasant’, while plant burgers were associated with ‘disappointed, distrust and discontented’52. Beyond and Impossible™ products introduce a new class of PBM products that more closely mimic ABM compared to the previously established texturized vegetable protein items. These products may be viewed as “highly processed” compared to traditional vegetable burgers and may alienate “clean label” consumers; who are concerned about “unnatural” methods of food production53. New consumer acceptance research is required to determine how these products measure up to the findings reported for more established products.
A general consensus in the field is that CBM is targeted at consumers who currently eat meat; as plant-based diets are agreed upon as healthful and sustainable for vegetarian-leaning individuals. Interestingly, vegetarians and vegans in the United States are both more likely to agree with the potential benefits of CBM but less willing to try it compared to omnivores54. A systematic review of cultured meat consumer acceptance studies found the most commonly reported concerns were associated with: unnaturalness, safety, healthiness, taste, texture, and price55. A 2017 European consumer study found that lack of naturalness decreased acceptance of cultured meat; even with awareness of potential environmental and animal welfare benefits56. Along with this finding, research examining internet comments on United States-based news articles covering cultured meat development found more critical input than approval responses, and a frequent critique was that CBM would be “unnatural” and “unappealing”57. A 2018 Switzerland study concluded that informing consumers about the production process did not increase acceptance and that communications that emphasize the final product, rather than the technical processes, would be a more successful strategy58. Similarly, a 2020 Netherlands study reported that educating consumers on the personal and societal benefits had a positive impact on consumer acceptance28. Nomenclature of meat grown from cell cultures may also have an effect on consumer perspective. When comparing the effects of ‘animal-free, clean, cultured’ and ‘lab-grown’ on a panel of participants, ‘animal-free’ and ‘clean’ incited more positive attitudes compared to ‘lab-grown’59. Other common descriptors include ‘artificial’, ‘cell-based’, ‘cultivated’, ‘in vitro’, and ‘synthetic’.
Meat is an important source of nutrition, especially in developing countries facing nutrition deficiencies. However, the overconsumption of meat has been linked to a number of health concerns. More than 1.8 million people die each year from ischemic heart disease, a quarter of which is linked with overconsumption of certain meat products60. Results from a recent clinical trial administered by the Stanford School of Medicine demonstrated that participants who substituted PBM for ABM over eight weeks exhibited lower risk for cardiovascular disease (e.g., reduced fasting serum trimethylamine-N-oxide levels)61. The consumption of PBM follows most nutritional dietary guidelines which recommend to limit intake of red and processed meat62,63, and could benefit consumers that desire reductions in blood pressure, body mass index, and cholesterol64. Foodborne pathogens found in meat, such as Escherichia coli, Salmonella, and Campylobacter, result in millions of illnesses each year65. Though PBMs are generally not associated with pathogenic disease concerns, non-animal products are capable of causing foodborne illness. A 1999 study screening tofu sold at grocery stores determined that 16% of tested samples were contaminated with coliform bacteria66. Plant foods can become contaminated with pathogens via contact with contaminated sources of animal manure, water or other foods. Antibiotics are also used in plant agriculture, but at relatively low levels (in the United States, plant use accounts for only 0.12% of animal agriculture antibiotic use)67, therefore, compared with ABM, PBM is less associated with antibiotic-related “drug-resistance” issue.
The commercialization of CBM could impact numerous aspects of public health including foodborne illness, nutrition deficiency, diet-related disease (e.g., colorectal cancer, cardiovascular disease), and infectious disease6. The risk of foodborne illness from CBM could be theoretically non-existent, since the sterile conditions required for cell proliferation will prevent contamination with disease-causing pathogens, provided that post-processing and packaging procedures are equally sterile. While sterile cell culture is implemented in pharmaceutical manufacturing, it may not be economically feasible for food production. Natural, food-grade antimicrobial agents may be a promising strategy to reduce contamination risk while remaining cost-effective68. Similarly, the threat of zoonotic disease transmission could be directly reduced by decreasing human contact with infected animals and indirectly by reducing habitat destruction69. Nutrition deficiency and diet-related disease could be addressed with cell selection, genetic modification, and medium formulation strategies to regulate the presence of healthful and unhealthful compounds. Conversely, consumers report concerns with the safety and healthfulness of CBM, citing fears about unnaturalness, cancer, and inadequate regulation55. So far, public health claims on both sides are entirely speculative as the relevant research has yet to be published.
Beyond Meat® and Impossible™ Foods have both released life cycle assessments (LCA) for their plant-based beef products70,71. Eutrophication potential and land use requirements for these products are projected to be significantly lower than metrics reported for factory-farmed animal-based beef, pork and chicken while greenhouse gas emissions fall between metrics for pork and chicken and energy consumption exceeds that of pork and chicken (Fig. 5). Compared to estimates for Beyond and Impossible™, mycoprotein (i.e., Quorn™) is more impactful with regard to energy and emissions but requires less land for production (Fig. 5). The water footprint of PBMs is highly dependent on the source of the main protein. A LCA comparing meat alternatives calculated that mycoprotein-based products (40 kg/kg) have higher water requirements compared to gluten (0.954 kg/kg) and soy-based (0.73 kg/kg) items72. A separate LCA study estimated the water usage for 39 distinct meat analogs and determined that, on average, a ton of PBM product consumed 3800 m3 of water73. A majority of the consumption is due to the processing of meat analogs after harvest of the raw protein sources (transportation and packaging were other factors).
The production of CBM is anticipated to, once optimized, require fewer resources and emit less waste relative to ABM14. Anticipated reductions are based on assumptions of: (1) targeted tissue cultivation (i.e., reduced by-products, non-meat tissues); (2) higher production rates and (3) vertical production systems14. The first relevant LCA published in 2011 estimated CBM would involve lower energy consumption (7–45%), greenhouse gas emissions (78–96%), land use (99%), and water use (82–96%) compared to ABM74. A separate 2015 LCA of CBM reported less dramatic footprint reductions and determined that the energy consumption, acidification potential, and ozone depletion potential impacts of CBM could be more detrimental than ABM, especially when compared to poultry production75. CBM is estimated to have a 47% energy feed conversion efficiency and 72% protein feed conversion efficiency, values that are lower than PBM and insect-based meat but higher than ABM76.
PBM products are generally free of animal byproducts and thus do not have direct negative impacts on animal welfare. However, a subset of products contains dairy-based or egg-based additives and thus are vegetarian but not vegan. Similar to the meat industry, egg, and dairy production methods are major sources of animal welfare concerns. In the egg industry, millions of male chicks that are not suitable for egg production or meat production, are killed each year and the beaks of female birds are trimmed to prevent pecking77. In the dairy industry, dairy cows are repeatedly impregnated for continuous milk production and are routinely separated from their calves, which are transported to other farms for veal production; causing extreme emotional distress78. Even vegan PBM can have indirect effects on wild animal welfare in the form of habitat destruction. To meet food demands, natural vegetation is cleared with monocultural crops which impacts biodiversity. In 1994, palm oil cultivation in Malaysia was found responsible for decreasing mammal inhabitants from 75 to 10 species per hectare79. While all agribusiness has an impact on animal welfare that are worthy of concern, substitution of ABM with PBM is still a substantial improvement for animal welfare, as it avoids the unethical treatment of animals during rearing, transportation and slaughter80.
One of the primary proposed benefits of CBM is the improvement of farm animal welfare via supplanting intensive animal agriculture. Animal donors are used to supply initial sources of cells that are subsequently expanded in vitro, without needing further resources from the animal. Donor animals, usually younger animals that have more proliferative cells, are anesthetized by a veterinarian and a small (<1 g) tissue biopsy is removed. Cells could be genetically immortalized to proliferate indefinitely; eradicating the need for animal donors. However, in practice, animal donors will likely be relied upon to maintain genetic diversity and to supply non-genetically modified options for CBM. Aside from cell sourcing, a key aspect of CBM production that is linked to animals is serum supplementation. Fetal bovine serum is a common additive to cell culture media and it provides essential growth factors for mammalian cell culture. In 2003, it was estimated that blood from 1,000,000 bovine fetuses was harvested to generate the annual production of 500,000 liters of fetal bovine serum81. Serum-free alternatives include co-culture approaches or supplementation with recombinant growth factors.
Animal-based meat production has evolved over thousands of years to supply the demand for affordable and appetizing food. Unfortunately, this feat is accompanied with unintended consequences for human health, natural resources, and the animals involved. Driven by both the rise in global meat demand and increased concern about the aforementioned negative externalities, researchers, and entrepreneurs are turning their focus towards animal-free approaches of meat production (Box 1). The economic opportunity for meat alternatives is large and there is no need to crown a single front-runner technology to monopolize the market. Instead, it is important to pursue multiple solutions simultaneously to provide a range of products to serve disparate segments of the consumer market. Plant-based and cell-based meat technologies have made significant advances since their conception. PBM has evolved from being a lackluster meat alternative, that provides a nutritional but not sensory replacement to meat, to be a novel analog nearly indistinguishable from the ABM it seeks to emulate. Likewise, CBM has matured from being the musing of science fiction to being a tangible prototype.
PBM products lie on a spectrum where one end houses more “natural,” less-processed proteins that fall in line with “clean-label” viewpoints but do not do well to mirror the experience of eating meat, while the other contains sensory equivalents that require source proteins to be entirely transformed, and thus viewed as highly processed, and which may come at the cost of certain nutritional factors. Mycelium-based meat may be an exception, where biofermentation can be employed to utilize natural structures and growth patterns of filamentous fungi to mimic meat structures. Along these lines, screening new protein sources that exist in nature that may emulate meat without excessive human manipulation may be an approach that appeals to a wider pool of consumers. CBM is impeded by high production costs, scale-up hurdles, and gaps in fundamental knowledge surrounding how to employ cell culture for food applications. In particular, there are no peer-reviewed, comprehensive datasets detailing the cost, sensory properties, or nutritional value of cell-cultured tissues. While published assessments of environmental impact projections exist, they are based on theoretical large-scale processes that have yet to be validated by industry. To that point, it will be necessary to ascertain details, such as the parameters of material inputs (e.g., doubling time, maximum cell density, medium composition) and industrial-scale production schematics (e.g., bioreactor design, operations) before cost, impact and food safety can be reliably analyzed.
There are opportunities for plant-based and cell-based hybrid products. Considering the current high-cost hurdles associated with CBM, one approach is to focus on the aspects of PBM that fall short of ABM and determine where CBM can add the most value at the lowest inclusion rate. For example, combining PBM with cell-cultured fat may improve the sensory properties of the analog while remaining less costly than a pure CBM product. To this end, expanding research goals to answer fundamental questions surrounding the cost, sensory and nutrition profiles is important to further inform stakeholders on the best areas of application for CBM. Finally, eventual success of PBM and CBM in the marketplace could transform, rather than eliminate, ABM production. If demand for lower quality, previously factory-farmed meat can be supplied with PBM and CBM, demand for higher quality, ABM could be met by smaller-scale, more sustainable and more humane methods of animal farming.
Godfray, H. C. J. et al. Meat consumption, health, and the environment. Science 361, eaam5324 (2018). Global meat consumption, driven upward by economic prosperity and population growth, negatively impacts human health and the environment.
Seto, K. C. & Ramankutty, N. Hidden linkages between urbanization and food systems. Science 352, 943–945 (2016).
Alexandratos, N. & Bruinsma, J. World Agriculture Towards 2030/2050: The 2012 Revision. Agricultural Development Economics Division (2012).
Waite, R. et al. Improving productivity and environmental performance of aquaculture. Creating a Sustainable Food Future. https://doi.org/10.5657/FAS.2014.0001 (2014).
Vranken, L., Avermaete, T., Petalios, D. & Mathijs, E. Curbing global meat consumption: emerging evidence of a second nutrition transition. Environ. Sci. Policy 39, 95–106 (2014).
Wolk, A. Potential health hazards of eating red meat. J. Intern. Med. 281, 106–122 (2017).
Jones, B. A. et al. Zoonosis emergence linked to agricultural intensification and environmental change. Proc. Natl Acad. Sci. USA 110, 8399–8404 (2013).
Hendrickson, M. K. Covid lays bare the brittleness of a concentrated and consolidated food system. Agric. Human Values. https://doi.org/10.1007/s10460-020-10092-y (2020).
Intergovernmental Panel on Climate Change. Special Report on Global Warming of 1.5 °C. (2018).
Steinfeld, H. et al. Livestock’s Long Shadow—Environmental Issues and Options. (Food and Agriculture Organization of the United Nations, 2006).
Komoroske, L. M. & Lewison, R. L. Addressing fisheries bycatch in a changing world. Front. Mar. Sci. 2, 83 (2015).
Tilman, D. et al. Future threats to biodiversity and pathways to their prevention. Nature 546, 73–81 (2017).
Ismail, I., Hwang, Y.-H. & Joo, S.-T. Meat analog as future food: a review. J. Anim. Sci. Technol. 62, 111–120 (2020).
Datar, I. & Betti, M. Possibilities for an in vitro meat production system. Innov. Food Sci. Emerg. Technol. 11, 13–22 (2010).
Joshi, V. K. & Kumar, S. Meat analogues: plant based alternatives to meat products—a review. Int. J. Food Ferment. Technol. 5, 107–119 (2015).
Krintiras, G. A., Gadea Diaz, J., Van Der Goot, A. J., Stankiewicz, A. I. & Stefanidis, G. D. On the use of the Couette Cell technology for large scale production of textured soy-based meat replacers. J. Food Eng. 169, 205–213 (2016).
Fraser, R. Z., Shitut, M., Agrawal, P., Mendes, O. & Klapholz, S. Safety evaluation of soy leghemoglobin protein preparation derived from pichia pastoris, intended for use as a flavor catalyst in plant-based meat. Int. J. Toxicol. 37, 241–262 (2018).
Smith, F. E. The World in 2030 A.D. (Hodder and Stoughton, 1930).
Van Eelen, W. F., Van Kooten, W. J. & Westerhof, W. Industrial scale production of meat from in vitro cell cultures. (1997). WO1999031222A1. https://patents.google.com/patent/WO1999031222A1/en.
Benjaminson, M., Gilchriest, J. & Lorenz, M. In vitro Edible Muscle Protein Production System (MPPS): Stage 1, Fish. Acta Astronaut 51, 879–889 (2002).
Asgar, M. A., Fazilah, A., Huda, N., Bhat, R. & Karim, A. A. Nonmeat protein alternatives as meat extenders and meat analogs. Compr. Rev. Food Sci. Food Saf. 9, 513–529 (2010).
Lusk, J. L. & Norwood, F. B. Some economic benefits and costs of vegetarianism. https://doi.org/10.1017/S1068280500003142 (2009).
Tziva, M., Negro, S. O., Kalfagianni, A. & Hekkert, M. P. Understanding the protein transition: the rise of plant-based meat substitutes. Environ. Innov. Soc. Transitions. https://doi.org/10.1016/j.eist.2019.09.004 (2019).
van der Weele, C. & Tramper, J. Cultured meat: every village its own factory? Trends Biotechnol. 32, 294–296 (2014). Small-scale, compared to large-scale, production of cell-based meat may be more technologically and socially feasible but economic hurdles represent a significant obstable.
Doyle, D., Omholt, S. W., Doyle, D., Vincent, J. & Angela, O. The In Vitro Meat Consortium Preliminary Economics Study. (The In Vitro Meat Consortium, 2008).
Rubio, N. R., Fish, K. D., Trimmer, B. A. & Kaplan, D. L. Possibilities for engineered insect tissue as a food source. Front. Sustain. Food Syst. 3, 24 (2019).
Rubio, N. R., Fish, K. D., Trimmer, B. A. & Kaplan, D. L. In vitro insect muscle for tissue engineering applications. ACS Biomater. Sci. Eng. 5, 1071–1082 (2019).
Rolland, N. C. M., Markus, C. R. & Post, M. J. The effect of information content on acceptance of cultured meat in a tasting context. PLoS ONE 15, e0231176 (2020). Personal and social benefit information, rather than information about quality and taste, may increase consumer acceptance of cell-based meat and a significant pool of consumers reports being willing to pay a premium price for cell-based meat over animal-based meat.
The US Cattlemen’s Association. Peition for the Imposition of Beef and Meat Labeling Requirements. FSIS Case No. 2018, 114 (2018).
Food and Drug Administration & U.S. Department of Agriculture Food Safety and Inspection Service. Formal Agreement Between the U.S. Department of Health and Human Services Food and Drug Administration and U.S. Department of Agriculture Office of Food Safety. (2019).
Schneider, Z. In vitro meat: space travel, cannibalism, and federal regulation. Houst. Law Rev. 50, 4067 (2013).
Stephens, N., King, E. & Lyall, C. Blood, meat, and upscaling tissue engineering: promises, anticipated markets, and performativity in the biomedical and agri-food sectors. Biosocieties 13, 368–388 (2018).
Penn, J. ‘Cultured meat’: lab-grown beef and regulating the future meat market. UCLA J. Environ. Law Policy 36 (2018).
Riley, J. & Mittenthal, E. Plant Based and Cultured Alternative Protein Products. (2019).
Petetin, L. Frankenburgers, risks and approval. Eur. J. Risk Regul. 5, 168–186 (2014).
Kyriakopoulou, K., Dekkers, B. & van der Goot, A. J. Plant-Based Meat Analogues. Sustainable Meat Production and Processing (Elsevier Inc., 2019).
Hsieh, Y. P. C., Pearson, A. M. & Magee, W. T. Development of a synthetic meat flavor mixture by using surface response methodology. J. Food Sci. 45, 1125–1130 (1980).
Wiebe, M. G. Quorn Myco-protein—Overview of a successful fungal product. Mycologist 18, 1–4 (2004). Quorn, based on filamentous fungi cultivation, is an early and sucessful alternative to minced meat and it presents nutrition benefits such as a favorable amino acid profile and a high fiber content.
Post, M. J. Cultured meat from stem cells: challenges and prospects. Meat Sci. 92, 297–301 (2012).
Sharma, S., Thind, S. S. & Kaur, A. In vitro meat production system: why and how? J. Food Sci. Technol. 52, 7599–7607 (2015).
Fish, K. D., Rubio, N. R., Stout, A. J., Yuen, J. S. K. & Kaplan, D. L. Prospects and challenges for cell-cultured fat as a novel food ingredient. Trends Food Sci. Technol. 98, 53–67 (2020).
Simsa, R. et al. Extracellular heme proteins influence bovine myosatellite cell proliferation and the color of cell-based meat. Foods 8, (2019). External supplementation of heme proteins can improve growth rates and the color of bioartifical muscles, produced by culturing bovine muscle cells within fibrin hydrogels.
Flaibani, M. et al. Muscle differentiation and myotubes alignment is influenced by micropatterned surfaces and exogenous electrical stimulation. Tissue Eng. Part A 15, 2447–2457 (2009).
Ben-arye, T. et al. Textured soy protein scaffolds enable the generation of three-dimensional bovine skeletal muscle tissue for cell-based meat. Nat. Food 1, 210–220 (2020). Textured soy protein scaffolds can support 3D culture of bovine skeletal muscle, smooth muscle and endothelial cells and the resultant constructs received favorable feedback from volunteer taste-testers.
Neacsu, M., Mcbey, D. & Johnstone, A. M. Meat reduction and plant-based food: replacement of meat: nutritional, health, and social aspects. https://doi.org/10.1016/B978-0-12-802778-3.00022-6 (2017).
Hu, F. B., Otis, B. O. & McCarthy, G. Can plant-based meat alternatives be part of a healthy and sustainable diet? JAMA https://doi.org/10.1001/jama.2019.13187 (2019).
Kadim, I. T., Mahgoub, O., Baqir, S., Faye, B. & Purchas, R. Cultured meat from muscle stem cells: A review of challenges and prospects. J. Integr. Agric 14, 222–233 (2015).
Saeki, K. et al. Functional expression of a delta12 fatty acid desaturase gene from spinach in transgenic pigs. Proc. Natl Acad. Sci. USA 101, 6361–6366 (2004).
Bryant, C., Szejda, K., Parekh, N., Desphande, V. & Tse, B. A Survey of Consumer Perceptions of Plant-Based and Clean Meat in the USA, India, and China. Front. Sustain. Food Syst. 3, 11 (2019). Consumers in India, compared to China and the United States, report higher rates of food neophobia and lower attachment to animal-based meat; acceptance of cultured meat is demonstrated to be higher in both China and India compared to the United States.
Hoek, A. C. et al. Replacement of meat by meat substitutes. a survey on person- and product-related factors in consumer acceptance. Appetite 56, 662–673 (2011).
Weinrich, R. Cross-cultural comparison between German, French and Dutch consumer preferences for meat substitutes. Sustainability 10, 1819 (2018).
Schouteten, J. J. et al. Emotional and sensory profiling of insect-, plant- and meat-based burgers under blind, expected and informed conditions. Food Qual. Prefer. 52, 27–31 (2016).
Asioli, D. et al. Making sense of the “clean label” trends: a review of consumer food choice behavior and discussion of industry implications. Food Res. Int. 99, 58–71 (2017).
Wilks, M. & Phillips, C. J. C. Attitudes to In Vitro Meat: A Survey of Potential Consumers in the United States. PLoS One 12, e0171904 (2017).
Bryant, C. & Barnett, J. Consumer acceptance of cultured meat: a systematic review. Meat Sci. 143, 8–17 (2018).
Siegrist, M. & Sütterlin, B. Importance of perceived naturalness for acceptance of food additives and cultured meat. Appetite 113, 320–326 (2017).
Laestadius, L. I. & Caldwell, M. A. Is the future of meat palatable? perceptions of in vitro meat as evidenced by online news comments. Public Health Nutr. 18, 2457–2467 (2015).
Siegrist, M., Sütterlin, B. & Hartmann, C. Perceived naturalness and evoked disgust influence acceptance of cultured meat. Meat Sci. 139, 213–219 (2018).
Bryant, C. J. & Barnett, J. C. What’s in a name? consumer perceptions of in vitro meat under different names. Appetite 137, 104–113 (2019).
Key, T. J., Davey, G. K. & Appleby, P. N. Health benefits of a vegetarian diet. Proc. Nutr. Soc. 58, 271–275 (1999).
Crimarco, A. et al. A randomized crossover trial on the effect of plant-based compared with animal-based meat on trimethylamine-N-oxide and cardiovascular disease risk factors in generally healthy adults: Study With Appetizing Plantfood — Meat Eating Alternative Trial (SWA. 1–12 (2020). Replacing animal-based meat with a plant-based meat analog, while controlling for the remainder of the diet, decreased cardiovascular disease risk factors in a trial following 36 healthy adult participants.
Craig, W. J. & Mangels, A. R. Position of the American Dietetic Association: vegetarian diets. J. Am. Diet. Assoc. 109, 1266–1282 (2009).
Fischer, C. G. & Garnett, T. Plates, pyramids, and planets: developments in national healthy and sustainable dietary guidelines: a state of play assessment. (Food and Agriculture Organization of the United Nations, 2016).
Tuso, P. J., Ismail, M. H., Ha, B. P. & Bartolotto, C. Nutritional update for physicians: plant-based diets. Perm. J. 17, 61–66 (2013).
Centers for Disease Control and Prevention. Estimates of foodborne illness in the United States. Atlanta Cent. Dis. Control Prev. (2011).
Ashraf, H., White, M. & Klubek, B. Microbiological Survey of Tofu Sold in a Rural Illinois County. J. Food Prot. 62, 1050–1053 (1999).
Stockwell, V. O. & Duffy, B. Use of antibiotics in plant agriculture. Rev. Sci. Tech. 31, 199–210 (2012).
Gyawali, R. & Ibrahim, S. A. Natural products as antimicrobial agents. Food Control 46, 412–429 (2014).
Ostfeld, R. S. Biodiversity loss and the rise of zoonotic pathogens. Clin. Microbiol. Infect. 15, 40–43 (2009).
Heller, M. C. & Keoleian, G. A. Beyond Meat’s Beyond Burger Life Cycle Assessment. (University of Michigan, Ann Arbor - Center for Sustainable Systems, 2018).
Khan, S., Dettling, J., Loyola, C., Hester, J. & Moses, R. Environmental Life Cycle Analysis: Impossible Burger 2.0. (Quantis, 2019).
Smetana, S., Mathys, A., Knoch, A. & Heinz, V. Meat alternatives: life cycle assessment of most known meat substitutes. Int. J. Life Cycle Assess. 20, 1254–1267 (2015).
Fresán, U., Marrin, D., Mejia, M. & Sabaté, J. Water footprint of meat analogs: selected indicators according to life cycle assessment. Water 11, 728 (2019).
Tuomisto, H. L. & Teixeira de Mattos, M. J. Environmental impacts of cultured meat production. Environ. Sci. Technol. 45, 6117–6123 (2011).
Mattick, C. S., Landis, A. E., Allenby, B. R. & Genovese, N. J. Anticipatory life cycle analysis of in vitro biomass cultivation for cultured meat production in the United States. Environ. Sci. Technol. 49, 11941–11949 (2015). Cell-based meat could require lower agricultural and land inputs compared to animal-based meat, but the process may be more energy intensive as biological functions are replaced by energy-consuming industrial processes.
Alexander, P. et al. Could consumption of insects, cultured meat or imitation meat reduce global agricultural land use? Glob. Food Sec. 15, 22–32 (2017).
Cheng, H. Morphopathological changes and pain in beak trimmed laying hens. Worlds Poult. Sci. J. 62, 41–52 (2006).
Ventura, B. A., von Keyserlingk, M. A. G., Schuppli, C. A. & Weary, D. M. Views on contentious practices in dairy farming: the case of early cow-calf separation. J. Dairy Sci. 96, 6105–6116 (2013).
Henson, I. E. Environmental impacts of oil palm plantations in Malaysia. PORIM Occas. Pap. 57 (1994).
Kumar, P. et al. Meat analogues: health promising sustainable meat substitutes. Crit. Rev. Food Sci. Nutr. 57, 923–932 (2017).
Gstraunthaler, G. Alternatives to the use of fetal bovine serum: serum-free cell culture. ALTEX 20, 275–281 (2003).
Khan, M. I., Jo, C. & Tariq, M. R. Meat flavor precursors and factors influencing flavor precursors—a systematic review. Meat Sci. 110, 278–284 (2015).
Dashdorj, D., Amna, T. & Hwang, I. Influence of specific taste-active components on meat flavor as affected by intrinsic and extrinsic factors: an overview. Eur. Food Res. Technol. 241, 157–171 (2015).
Adams, A., Bouckaert, C., Van Lancker, F., De Meulenaer, B. & De Kimpe, N. Amino acid catalysis of 2-alkylfuran formation from lipid oxidation-derived α,β-unsaturated aldehydes. J. Agric. Food Chem. 59, 11058–11062 (2011).
Van Boekel, M. A. J. S. Formation of flavour compounds in the Maillard reaction. Biotechnol. Adv. 24, 230–233 (2006).
Arnold, R. G., Libbey, L. M. & Lindsay, R. C. Volatile flavor compounds produced by heat degradation of thiamine (vitamin B1). J. Agric. Food Chem. 17, 390–392 (1969).
Castle, L. A., Wu, G. & McElroy, D. Agricultural input traits: past, present and future. Curr. Opin. Biotechnol. 17, 105–112 (2006).
Shurtleff, W., Huang, H. T. & Aoyagi, A. History of Soybeans and Soyfoods in China and Taiwan, and in Chinese Cookbooks, Restaurants, and Chinese Work with Soyfoods Outside China (1024 BCE to 2014): Extensively Annotated Bibliography and Sourcebook, Including Manchuria, Hong Kong and Tibet. (Soyinfo Center, 2014).
Gul, T., Haq, E. & Balkhi, H. Basics of In Vitro Cell Culture. in Evaluation of Cellular Processes by In Vitro Assays (eds. Gul, T., Haq, E. & Balkhi, H.) 2 (Bentham Science Publishers, 2018).
Kirchhelle, C. Pharming animals: a global history of antibiotics in food production (1935–2017). Palgrave Commun. 4, 1–13 (2018).
Bhat, Z. F. & Fayaz, H. Prospectus of cultured meat—advancing meat alternatives. J. Food Sci. Technol. 48, 125–140 (2011).
Nierenberg, D. Factory Farming in the Developing Word. World Watch 10–19 (2003).
Pobiner, B. Evidence for meat-eating by early humans. Nat. Educ. Knowl. 4, 1 (2013).
Food and Drug Administration. Daily Value on the New Nutrition and Supplement Facts Labels. (2020).
We thank our lab team members (Andrew, Kyle, John) for stimulating and productive discussions. We thank New Harvest, the Good Food Institute, the Advanced Research Projects Agency, and the National Institutes of Health (P41EB027062) for support.
The authors declare no competing interests.
Peer review information Nature Communications thanks the anonymous reviewer(s) for their contribution to the peer review of this work.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Rubio, N.R., Xiang, N. & Kaplan, D.L. Plant-based and cell-based approaches to meat production. Nat Commun 11, 6276 (2020). https://doi.org/10.1038/s41467-020-20061-y
Life cycle assessment of edible insects (Protaetia brevitarsis seulensis larvae) as a future protein and fat source
Scientific Reports (2021)
Environmental and Resource Economics (2021)
Food Security (2021)