Leishmaniasis is a neglected tropical disease caused by Leishmania protozoa transmitted by infected sand flies. Vaccination through leishmanization with live Leishmania major has been used successfully but is no longer practiced because it resulted in occasional skin lesions. A second generation leishmanization is described here using a CRISPR genome edited L. major strain (LmCen−/−). Notably, LmCen−/− is a genetically engineered centrin gene knock-out mutant strain that is antibiotic resistant marker free and does not have detectable off-target mutations. Mice immunized with LmCen−/− have no visible lesions following challenge with L. major-infected sand flies, while non-immunized animals develop large and progressive lesions with a 2-log fold higher parasite burden. LmCen−/− immunization results in protection and an immune response comparable to leishmanization. LmCen−/− is safe since it is unable to cause disease in immunocompromised mice, induces robust host protection against vector sand fly challenge and because it is marker free, can be advanced to human vaccine trials.
Leishmaniasis is a neglected disease caused by infection with protozoans of the genus Leishmania that is transmitted by infected sand flies1. Worldwide, an estimated 1 billion people are at risk of infection in tropical and subtropical countries where up to 1.7 million new cases in 98 countries occur each year2,3. The disease pathology ranges from localized skin ulcers (cutaneous leishmaniasis, CL) to fatal systemic disease (visceral leishmaniasis, VL), depending on the species of the infecting Leishmania parasite1,4. Treatment options for both VL and CL are limited and there is poor surveillance in the most highly endemic countries1,5. A prophylactic vaccine would be an effective intervention for protection against this disease, reducing transmission and supporting the elimination of leishmaniasis globally. Currently there are no available vaccines against any form of human leishmaniasis.
Unlike most parasitic infections, patients who recover from leishmaniasis naturally or following drug treatment develop immunity against reinfection indicating that the development of an effective vaccine should be feasible6,7,8. Furthermore, leishmanization, a process in which deliberate infections with a low dose of virulent Leishmania major provides greater than 90% protection against reinfection and has been used in several countries of the Middle East and the former Soviet Union9,10,11. Leishmanization is however no longer practiced due to safety concerns regarding skin lesions that last for months at the site of inoculation. The overall strategy of this study is to develop the next generation leishmanization that is safer by providing a protective immune response against cutaneous leishmaniasis without causing skin lesions.
In case of leishmaniasis cell-mediated immunity is critical, and particularly, CD4 T cells play a crucial role in the protection against CL12. Specifically, host defense involves Th1 response due to T- cells primed by antigen-presenting cells producing IL-1213. Production of IL-12 by antigen-presenting cells and IFNγ by T cells are crucial for controlling the parasite numbers13. In contrast, Th2 cytokines, mainly IL-4, IL-5 and IL-13, anti-inflammatory cytokines, suppress host immunity and help parasite survival while minimizing the tissue damage due to unchecked inflammation13,14. The differential effects of Th1 and Th2 dichotomy in cutaneous leishmaniasis is extensively studied in murine models15.
Studies with several candidate vaccines against CL including leishmanization have demonstrated that the establishment of predominant Th1 type of immune response correlated with protection16,17,18. In murine leishmanization models, it is well established that IFN-γ producing CD4 Th1 cells are essential in mediating protective immunity against re-infection19,20. Multifunctional effector Th1 cells which also produce high IFN-γ play a crucial role in host protection21. Recently it has been shown in leishmanized mice that rapidly recruited short-lived effector T cells producing IFN-γ confer significant level of protection and could be used as a biomarker of host protection22,23. These studies collectively show that any effective vaccine should similarly maintain these antigen specific CD4 T cell populations long enough to induce a robust protection against reinfection.
Centrin is a calcium-binding protein and essential in the duplication of centrosomes in eukaryotes including Leishmania24,25. Previously, we have shown that centrin gene-deficient Leishmania donovani parasites are viable in axenic promastigote culture but do not proliferate in infected macrophages and are highly efficacious as a live vaccine in animal models26,27,28,29,30,31. However, using live-attenuated L. donovani as a vaccine in humans is high-risk because of the potential for visceralization resulting in fatal visceral disease. Further, previously generated gene deleted L. donovani strains required the incorporation of antibiotic resistance marker genes. The presence of antibiotic resistance genes in any attenuated live vaccine renders the vaccine unacceptable by regulatory agencies for human vaccine trials.
To overcome these drawbacks, we used CRISPR-Cas genome editing recently established for Leishmania32,33,34 to generate an attenuated L. major centrin gene deletion mutant (LmCen−/−). This represents a major milestone because LmCen−/− contains no antibiotic-resistant selection genes, an essential prerequisite for approval by regulatory agencies and advancement to human trials. L. major was used because this species is safer than L. donovani since L. major remains in the skin at the site of infection and does not cause visceral disease1,4. As demonstrated within, vaccination with LmCen−/− is safe, immunogenic and protective against sand fly transmitted L. major infection, that mimics natural infection in highly relevant cutaneous leishmaniasis animal models meeting efficacy and ethical standards for advancement to human clinical studies.
Generation of centrin deficient LmCen −/− by CRISPR-Cas
CRISPR-Cas genome editing has recently been developed to delete Leishmania genes with or without integration of antibiotic selection markers into the genome32,33,34. The experimental approach used to delete the centrin gene (Gene ID: LmjF.22.1410) from L. major is detailed in Fig. 1a–d. Two guide sequences targeted to the 5′ and 3′ flanking sequences of the centrin gene were designed and cloned into the Leishmania CRISPR vector pLdCN33,34 (Fig. 1a) and transfected into L. major (Friedlin V9) promastigotes. To delete the centrin gene sequence precisely at the locations determined by the 2 guide RNA sequences flanking the centrin gene without using marker gene replacement, a 50-nucleotide oligonucleotide donor DNA sequence was transfected into the promastigotes containing the CRISPR expression vector pLdCNa&b as previously described33. The donor DNA consisted of 25 nucleotides 5′ from the upstream gRNAa cleavage site and 25 nucleotides 3′ from the downstream gRNAb cleavage site (Fig. 1b). The exact targeted sequences flanking the centrin gene and diagnostic PCR primers are shown in Supplementary Fig. 1A.
L. donovani centrin null promastigotes proliferate slower than wildtype promastigotes35. Since centrin-null promastigotes were selection marker free, this slower proliferation phenotype was used to identify centrin null L. major promastigotes. The CRISPR-genome edited L. major promastigotes were subjected to single-cell cloning in 96 well plates; the relatively slow-growing clones were identified, expanded and subjected to PCR analysis with the primers flanking the centrin gene as shown in Fig. 1b. An example of a PCR analysis of a slow-growing clone with the loss of the centrin gene is shown in Fig. 1c. Sequence analysis of the 604 bp PCR product shown in Fig. 1c confirmed the centrin gene-containing sequence was precisely deleted at the predicted gRNA target sites and the chromosome fused through the donor sequence as intended (Fig. 1d). The gRNA/Cas9 expressing pLdCNa&b plasmid was subsequently removed from the L. major centrin null mutant (LmCen−/−) by single-cell cloning and maintaining replica cultures in the presence and absence of G418 to identify clones sensitive to G418 that had lost the neomycin resistance gene present in the pLdCN CRISPR gene-editing plasmid. It was not possible to amplify plasmid DNA from the G418 sensitive LmCen−/− parasite providing further evidence for the loss of the pLdCNa&b plasmid (Supplementary Fig. 1B). As also shown in Supplementary Fig. 1C, the LmCen−/− parasite retained the phenotype of slower proliferation than the WT L. major. This difference in proliferation enabled the identification and isolation of the slower growing centrin gene deleted clones by visual and microscopy inspection of the 96 well plate after one week in culture.
LmCen −/− does not produce lesions in infected mice
It was necessary to establish whether the LmCen−/− had lost the ability to cause cutaneous infections and whether adding back the centrin gene through plasmid transfection (add-back, LmCen−/−AB) could restore cutaneous infection. The centrin gene was inserted into the Leishmania pKSNeo expression plasmid36,37, transfected into LmCen−/− promastigotes and expression of the centrin protein was confirmed by Western blotting with an α-LdCen antibody that can recognize L. major centrin (Fig. 1e). LmCen−/− infection was investigated following intradermal injection of 1 × 106 stationary phase promastigotes in the ear of C57BL/6 mice. As shown in Fig. 1f, by 5-6 weeks, LmCen−/− failed to produce swelling in the infected ear whereas wildtype L. major (LmWT) Friedlin V9 and the LmCen−/− with the add-back centrin gene (LmCen−/−AB) did induce significant swelling. At 6 weeks following infection, the LmCen−/− infected mice had few (<10) detectable parasites compared to both the LmWT and LmCen−/−AB infected mice that both had significantly more parasites (~2 × 106) (Fig. 1g). These observations confirm that at 6 weeks post-infection, marker-free LmCen−/− is unable to induce pathology at the site of injection in mice and that this was due to the deletion of the centrin gene.
We next examined LmCen−/− survival in human macrophages in vitro since these are the obligate host cells for intracellular replication of Leishmania amastigotes (Supplementary Fig. 1D). At 24 h post-infection, the number of parasites per macrophage was similar in the LmCen−/− and LmWT infected cells. However, by 8 days, LmCen−/− amastigotes were cleared from the macrophages, whereas LmWT parasites reached >10 parasites/macrophage. These results demonstrated that the LmCen−/− promastigotes effectively infected human macrophages but subsequently were unable to proliferate intracellularly.
LmCen −/− contains no off-target gene deletions
Since the CRISPR generated LmCen−/− strain was attenuated, it was necessary to establish the integrity of the genome by whole-genome sequencing analysis to confirm the attenuation seen was solely due to the removal of the centrin gene. This analysis confirmed that the targeted ~1 kb genome region containing the 450 bp centrin gene (ID:LmjF.22.1410) was deleted from chromosome 22 and the remaining centrin gene homologs on chromosomes 7, 32, 34 and 36 remained intact in the genome (Fig. 2a). Southern blot analysis confirmed the targeted centrin gene in LmCen−/− was deleted and not translocated to another region of the genome (Fig. 2b). Whole-genome sequencing was performed to establish whether there were any off-target gene deletions in the edited genome. As shown in Fig. 2c, the blue line is comprised of over 8,000 circles, each circle representing a single gene from chromosome 1 through 36 (left to right) whereas the red circles represent the members of the centrin gene family located on chromosomes 7, 22, 32, 34 and 36. There was virtually 100% coverage for all 8307 genes in the genome indicating the absence of partial or complete gene deletions, except the targeted centrin (LmjF.22.1410) that had a 0% coverage since it was deleted through CRISPR gene editing. A handful of genes (open blue circles) with less than 100% coverage are tandem repeat genes for which the coverage calculation software misaligned some reads, these genes were manually inspected and were found to be intact. Compared to the L. major Friedlin reference genome, there were no indels and no new SNPs (21 genes contained SNPs that were all previously identified in resequencing of the L. major Friedlin or LV39 strains). Collectively, these analyses demonstrate that the LmCen−/− genome is intact and has no off-target gene mutations.
The genomic DNA sequence reads were also searched for the presence of pLdCN CRISPR plasmid DNA sequence to confirm the loss of this plasmid. As shown in Fig. 2d, the only LmCen−/− genomic DNA sequence in common with the pLdCN CRISPR plasmid was the A2 gene intergenic sequence (A2-IGS) that is part of a A2 pseudogene sequence present in the L. major genome. The A2-IGS sequence from L. donovani was incorporated into the pLdCN CRISPR plasmid for processing of the NeoR gene transcript32. There were no other detectable plasmid sequences or antibiotic resistance genes in the genome of LmCen−/−. It is noteworthy that the L. donovani ribosomal RNA promoter (rRNAP) sequence in the pLdCN CRISPR plasmid is sufficiently divergent from the L. major rRNAP sequence that it was not identified in the MiSeq DNA sequences by the Maximal Exact Match (bwa-mem) sequence alignment algorithm used. Taken together, the results presented in Fig. 2d and Supplementary Fig. 1B in combination with the loss of G418 resistance demonstrate that the pLdCN CRISPR gene-editing plasmid is no longer present in LmCen−/−. This represents a significant milestone since LmCen−/− is the first marker-free gene deleted Leishmania strain to be generated in the laboratory.
LmCen −/− is safe and does not cause lesions in mice
As shown in Fig. 1, LmCen−/− was unable to induce ear cutaneous lesions in C57BL/6 mice due to the removal of the centrin gene. However, to assess the safety of LmCen−/− as a potential live vaccine, it was necessary to investigate its attenuation in a more susceptible mouse strain (BALB/c) and in immune-deficient mice. BALB/c mice injected subcutaneously in the footpad with 1 × 107 stationary phase LmCen−/− showed no footpad swelling over 20 weeks (Fig. 3a), the study endpoint, and a significantly lower parasite burden (approximately 4 log fold reduction) as compared to BALB/c mice injected with LmWT (Fig. 3a). In some animals LmCen−/− parasites were completely cleared by the study end point. Likewise, STAT-1 KO immune-deficient mice injected with 2 × 108 LmCen−/− stationary phase parasites showed no footpad swelling during 7 weeks following injection (Fig. 3b) whereas footpad swelling started at 4 weeks after injection with LmWT (Fig. 3b). The parasite burden at 7 weeks in STAT-1 KO mice injected with LmWT was significantly higher than the mice injected with LmCen−/− attenuated parasites (~6 log fold reduction, Fig. 3b). In another test, IFN-γ KO mice showed severe footpad swelling accompanied by a drastic increase in the number of the parasites after injection with 1 × 107 LmWT, while injection with the same dose of LmCen−/− did not show any footpad swelling in 20 weeks and the parasites were cleared from the site of injection (Fig. 3c). The recombination activating gene 2 deficient (Rag2 KO) mice, which lack conventional T cells and B cells, showed mild footpad swelling and a high parasite burden in the footpad after 15 weeks following injection with LmWT (Fig. 3d). In contrast, injection with LmCen−/− did not show any swelling (Fig. 3d; Supplementary Fig. 2C) and the parasites were cleared from the site of injection (Fig. 3d) and the spleen and liver (Supplementary Fig. 2D). These results demonstrate that LmCen−/− is non-pathogenic even in highly immunocompromised mice.
To rule out the survival of any undetectable LmCen−/− parasites beyond 7 weeks post-immunization, LmCen−/− infected BALB/c mice were treated with 2 mg/kg dexamethasone (DXM), a known immune suppressor, three times for a week starting at 10 weeks post-infection (Fig. 3e). All the groups were sacrificed at 4 weeks after the DXM treatment to determine parasite burdens. As shown in Fig. 3f and Supplementary Fig. 2A, LmCen−/− infected mice with or without DXM treatment resulted in no lesions while LmWT infected but DXM-untreated mice developed open ulcerative lesions in the ear. Moreover, only 2 of 12 DXM-treated mice infected with LmCen−/− showed parasites in the inoculated ear (Fig. 3g) and draining lymph node (Fig. 3h). In 1 of 6 untreated LmCen−/−-immunized animals, a low parasite number was detected in the draining lymph node (<100 parasites, Fig. 3h), and none in the ear (Fig. 3g). In LmWT infected mice, a significantly higher parasite load was observed in the ear and draining lymph node compared to LmCen−/− -infected mice (± DXM) (Fig. 3g, h), which correlated with ear lesion size (Fig. 3f). Further, a PCR analysis using L. major centrin gene-specific primers confirmed the absence of the centrin gene in the parasites isolated from DXM-treated mice (Supplementary Fig. 2B lane 1, red arrow). Collectively, these results revealed that the centrin deleted live LmCen−/− parasites are unable to revert or cause pathology and are safe for further study as a live vaccine.
LmCen −/− immunization protects against wildtype L. major
To investigate the protective efficacy of LmCen−/− against wildtype L. major, both resistant (C57BL/6) and susceptible (BALB/c) mice were immunized with a single intradermal (i.d.) injection with 1 × 106 stationary phase LmCen−/− in one ear. Seven weeks post-immunization, mice were challenged with 750 metacyclic wildtype L. major (WR 2885 strain) parasites in the contralateral ear via the i.d. route (Fig. 4a for C57BL/6; Supplementary Fig. 3A for BALB/c). Following challenge with wildtype L. major, lesion development was assessed up to 10 weeks for the C57BL/6 mice (Fig. 4b, c). In the non-immunized-challenged group, mice developed a non-healing open ulcer that progressively increased in size (Fig. 4c). No open ulcers were observed in the LmCen−/−-immunized-challenged group and only a moderate swelling that subsided from 5 to 9 weeks post-challenge was observed in 6 of 13 mice. Figure 4c depicts the ear pathology at 10 weeks post-challenge compared to a naïve unchallenged ear. Importantly, histopathological analysis revealed no clear difference between immunized-challenged and naïve mice ears, while non-immunized-challenged mice ears developed large lesions with open ulcers involving an influx of inflammatory cells (Fig. 4c). The parasite load in the challenged ear and draining lymph node were also quantified at 10 weeks post-challenge revealing that the immunized group had a significantly lower parasite load (approximately a 4-log fold and a 3.2-log fold reduction, respectively) compared to the non-immunized group (Fig. 4d, e). Similarly, highly susceptible BALB/c mice were protected following immunization with LmCen−/− parasites (Supplementary Fig. 3A–E). At 10 weeks post-challenge with wildtype L. major parasites, immunized BALB/c mice were protected as measured both by a reduced lesion size (Supplementary Fig. 3B, C) and parasite burden (Supplementary Fig. 3D, E) compared to non-immunized-challenged mice. A similar lack of non-healing open ulcer was observed in LmCen−/− immunized BALB/c mice challenged with other wildtype strains of L. major such as L. major FV9 (Supplementary Fig. 3F) and L. major LV39 (Supplementary Fig. 3G).
LmCen −/− protects against sand fly transmitted L. major WT
It is substantially more difficult and more relevant to demonstrate immunological protection against L. major infection initiated by a sandfly challenge than by a needle injection challenge20,38. Therefore, to determine the efficacy of LmCen−/− immunization against sand fly transmitted cutaneous infection by L. major, C57BL/6 mice were immunized with a single i.d. injection of 1 × 106 LmCen−/− stationary phase parasites and mice were infected by exposure to bites of 10 L. major-infected sand flies in the contralateral ear 7 weeks post-immunization (Fig. 5a). Disease progression was monitored for 10 weeks post-challenge by measuring lesion growth and assessing parasite burden in the ear and draining lymph node (Fig. 5b–e). Notably, only 1/12 immunized-challenged mice developed a visible lesion, while 10/14 non-immunized-challenged mice developed progressive lesions in the ear that were significantly larger than the single lesion observed in immunized-challenged mice (Fig. 5b, c). At 10 weeks post-challenge, there was a significant reduction of the parasite burden both in the ear and draining lymph node (approximately 2 log fold reduction in both) of immunized-challenged mice compared to non-immunized-challenged mice (Fig. 5d, e). It is interesting to note that some of the draining lymph nodes in the immunized-challenged mice did not have any parasites (Fig. 5e). These results demonstrate that immunization with LmCen−/− mediates significant protection under natural conditions of infection i.e., parasite transmission by an infected sand fly.
LmCen −/− or leishmanization induced similar immunological protection
Previously, in murine leishmanization models, it was shown that leishmanization induces host protective immunity against re-infection19,20. Having shown above that LmCen−/− induces protection against both needle and the natural model of sand fly challenge, we first compared the immune response between LmCen−/− immunized group (8 weeks of post-immunization) and a primary LmWT infection (healed group) at 12-weeks of post-primary infection (Fig. 6a). The rationale for using two different time points is in leishmanization group, mice infected with wild type L. major causes lesion development from 4 to 8 weeks and by 10–12 weeks most of the lesions are healed and very few parasites are present. On the contrary in the case of LmCen−/− parasite immunized animals they do not develop any active lesions, and beyond 6 weeks post-immunization we do not recover LmCen−/− parasites in most mice.
The ears from the healed group showed lesions that later resolved. In the LmCen−/− immunized group however, no lesion development was observed (Supplementary Fig. 4A, B). Antigen experienced CD4+ T cells were first gated based on their surface expression of CD44 and CD4+CD44+ cells were rearranged into different subpopulations based on their production of TNF-α, IFN-γ, and IL-2 (Fig. 6b). Fluorescence minus one (FMO) control was used for proper gating of positive events for designated cytokines (Supplementary Fig. 4C). The results showed that both LmCen−/− immunization and healed groups of mice induced comparable single as well as multiple cytokines secreting CD4+CD44+T cells upon re-stimulation with L. major freeze-thaw antigen (LmFTAg) (Fig. 6c, d). Naïve mice (not immunized with LmCen−/− or infected with LmWT) did not show any detectable immune response after antigen stimulation (Fig. 6c). Upon challenge with wildtype L. major parasites by needle injection, at 20 h post-infection, we observed a significant increase in the mRNA levels of IFN-γ in both healed and LmCen−/− immunized ear tissues compared to non-immunized mice (Fig. 6g). From the same time point after challenge (20 h post-infection), we also analyzed the IFN-γ production from effector CD4 T cells by flow cytometry. Both healed and LmCen−/− immunized mice induced a significantly higher percentage of IFN-γ+ effector T cells (Live CD4+CD44HiLy6C+T-bet+) compared to the non-immunized group (Fig. 6f). Figure 6e shows common gating strategies for early immune response (Live CD4+CD44Hi-Ly6C+ T-bet+IFN-γ+-T cells) in the ear of non-immunized, healed and LmCen−/− immunized mice at 20 h post needle challenge with wildtype L. major parasites. FMO control was used for proper gating of positive events for designated cytokines (Supplementary Fig. 4D).
Healed and LmCen−/− immunized groups were also challenged with L. major WT infected sand fly and the parasite loads were determined (Fig. 6h, i). After five weeks of post-challenge, there was a similar significant reduction of parasite burden in the ear (2.4 log fold in healed group, and 2.1 log fold in LmCen−/− immunized group) and draining lymph nodes (1.7 log fold in healed group, and 1.48 log fold in LmCen−/− immunized group) compared to non-immunized group (Fig. 6h, i). Both healed and LmCen−/− immunized-challenged mice did not develop any lesions whereas non-immunized-challenged mice developed cutaneous lesions (Supplementary Fig. 4E). Taken together, these results demonstrate that LmCen−/− immunization is as effective as leishmanization (LmWT infection/healed) in generating a protective immune response and protecting against sand fly mediated infection with WT L. major.
Leishmanization with wildtype L. major has so far been the only successful human vaccine for leishmaniasis but it is discontinued due to safety concerns associated with administration of a live virulent organisms. This paper describes a second generation leishmanization live vaccination with an attenuated L. major strain (LmCen−/−) that does not cause lesions but retains the ability to provide immunological protection against experimental needle and sand fly transmitted Leishmania infection. As LmCen−/− is marker gene free safe and efficacious, can be advanced to Phase I human clinical trials.
CRISPR-Cas genome editing was essential to generating this marker-free strain because this technology can delete genes with high specificity and fidelity without selection with antibiotic-resistant marker genes32,33,34. In place of antibiotic marker selection, the selection was based on a reduced proliferation rate of the LmCen−/− mutant identified through single-cell cloning, the first time such a selection has been performed in Leishmania. Whole-genome sequence analysis confirmed that only the centrin gene on chromosome 22 (ID:LmjF.22.1410) was precisely deleted at the CRISPR guide RNA targeting sites and other centrin gene members on chromosomes 7, 32, 34 and 36 remained intact. There were no CRISPR-induced off-target gene deletions, indels or nonsynonymous SNPs introduced in the LmCen−/− clone that was subjected to whole-genome sequencing. By comparison, a previously engineered L. donovani centrin gene deleted parasites generated by homologous recombination with antibiotic-resistant marker genes did contain off-target genomic deletions of up to 5000 base pairs in non-coding regions and in the coding regions of the folate transporter and gp63 genes39. Although gene-targeting specificity will depend largely on the selection of the guide RNA sequence, these observations suggest that CRISPR-Cas gene editing in Leishmania using a donor DNA fragment for repair as detailed in Fig. 1 is more specific than traditional homologous recombination-based gene replacement with antibiotic resistance markers. In theory, it could have also been possible to generate a centrin gene deleted markerless L. major parasite using a different CRISPR approach involving the transfection of recombinant SaCas9 protein with in vitro –transcribed guide RNAs directed to upstream and downstream sequences flanking the centrin gene40. Although deletion of other Leishmania virulence genes may likewise generate attenuated strains, the centrin gene was targeted in this study because centrin gene deleted L. donovani parasites have been the most extensively validated parasites in previous experimental vaccine studies using various animal models26,27,28,29,30,31. It is noteworthy that in this study, L. major was used instead of the previous studies involving centrin deleted L. donovani26,27,28,29,30,31 as the focus of this study was cutaneous leishmaniasis.
Numerous experimental vaccines have been developed for Leishmania, though most of them have not been tested against natural sand fly transmitted infections. In studies when such vaccines were tested by needle challenge versus sand fly transmission of a virulent parasite, they were either partially protective or not protective against the latter20,38,41,42. In addition, sand fly mediated infection provides other components present in the saliva which play an important role in the pathogenesis of Leishmania43,44,45. The observations reported here demonstrated that markerless LmCen−/− immunization did induce protection against sand fly transmitted L. major. In this study, a major obstacle to using a live vaccine, the risk of disease development, was overcome by engineering a markerless second generation live-attenuated parasite that can confer protection without associated pathology. Live attenuated LmCen−/− parasites elicited protective immunity in both susceptible (BALB/c) and resistant (C57BL/6) mice and against different strains of L. major (WR 2885, FV9 and LV39). Importantly LmCen−/− parasites elicited protection against sand fly challenge that was deemed necessary but was neither performed or was not demonstrated in previous vaccination studies20,38,41,42. These observations using a cutaneous model of infection are consistent with our previous findings that immunization with LdCen−/− parasites were protective against visceral leishmaniasis in different animal models26,27,28,30,46.
In previous studies evaluating Leishmania vaccines, researchers have used mice with healed cutaneous lesions following a low dose of wildtype L. major infection as a gold-standard animal model that mimics leishmanization in humans23,38,47. In this study, we have also compared LmCen−/− parasite immunization induced immunity with wildtype L. major infected, healed mice (leishmanization). Our results demonstrated comparable immune responses in mice either healed from wildtype infection or immunized with LmCen−/−. It has been shown that chronic parasite infection maintains Ly6C+CD4+ effector T cells, and upon challenge with LmWT parasites these are essential for IFN-γ production that mediates protection22. Our results established that upon challenge with LmWT parasites, both LmCen−/− immunized and healed mice generated a comparable percentage of CD4+Ly6C+IFN-γ+ effector T cells. In addition, protection may also be mediated by the circulating effector cells that are recruited by the tissue-resident memory T cells (Trm) immediately after challenge as was shown in a leishmanization mouse model47. Future studies with LmCen−/− will address the role of Trm cell as well as other memory phenotype T cells in LmCen−/− vaccine immunity. Moreover, upon L. major infected sand fly challenge, both groups are protected, and the levels of protection are comparable in terms of parasite burden. The residual parasite burden observed in both ear and lymph nodes in the LmCen−/− may be important for maintaining long term protection as was reported in previous studies with leishmanized mice22,48. However, unlike leishmanization which involved inoculation of low dose of virulent parasites that caused lesions at the site of injection, immunization with LmCen−/− parasites is safe as demonstrated by the absence of visible lesions in susceptible and immunodeficient animals post-immunization, in spite of persistence of a low number of LmCen−/− parasites at the site of inoculation.
In conclusion, this study demonstrated that LmCen−/− parasites are safe and can protect against a sand fly challenge with a wildtype L. major infection in relevant mouse models. Future studies are required to establish whether vaccination with LmCen−/− is safe and protective in humans. The combination of old (leishmanization) and new (CRISPR gene editing) technologies can result in major advances in vaccine design that has the potential to protect millions of people from this major neglected disease.
Leishmania strain and culture medium
L. major Friedlin (FV9) and L. major LV39 used in this study were routinely passaged into the footpads of BALB/c mice. Amastigotes isolated from infected lesions were grown in M199 medium and promastigotes were cultured at 27 °C in M199 medium (pH 7.4) supplemented with 10% heat-inactivated fetal bovine serum, 40 mM HEPES (pH 7.4), 0.1 mM adenine, 5 mg l−1hemin, 1 mg l−1 biotin, 1 mg l−1 biopterin, 50U ml−1 penicillin and 50 µg ml−1 streptomycin. Cultures were passaged to fresh medium at a 40-fold dilution once a week. The growth curve of L. major promastigotes was obtained by inoculating the parasite at 1 × 106/ml into the 96 well plate (150 µl/well) in quadruplicate, the OD values were measured once a day for 4 days.
L. major WR 2885 strain was used to infect sand flies and grown at 27 °C in Schneider’s medium supplemented with 10% heat-inactivated FCS, penicillin (100 U/ml), streptomycin (100 µg/ml), 2 mM l-glutamine.
The WR2885 strain was isolated in August 29, 2008 from a lesion on the right upper arm of a US soldier at the Walter Reed hospital, Bethesda, MD. It was acquired in Iraq and the cloned line was identified as L. major by polymerase chain reaction (PCR) and by isozyme assessment by a College of American Pathologists certified laboratory. The WR2885 strain is shown to have superior colonization and transmissibility by sand flies to mice resulting in more severe pathology (larger lesion size and higher parasite loads)38,49.
CRISPR plasmid construction
The pLdCNLm221410a&b plasmid vector was generated as follows: 1) A 276 bp PCR fragment containing gRNALm221410a, hepatitis delta virus and hammerhead ribozymes and gRNALm221410b guide coding sequences was amplified with primers Lm221410a and Ld221410b from the gRNA 241510+MT co-expression vector previously described33,34 2). The PCR product from step 1 was digested with Bbs I and inserted into the Bbs I digested pLdCN vector33,34 to generate the pLdCNLm221410a&b plasmid vector which was verified by sequencing analysis at the McGill University and Genome Quebec Innovation Center.
Guide RNA sequences and the oligonucleotide donor used in this study are listed below and their locations in the centrin gene (LmjF.22.1410) locus are indicated in the Supplementary Fig. 1.
Lm221410a (containing gRNAa guide coding sequence): 5′ATCGAAGACCTTTGTCTTCTCGCAATCCTTCTGCTGTTTTAGAGCTAGAAATAGCAAG
Lm221410b (containing gRNAb guide coding sequence): 5′ATCGAAGACCCAAACTTGAGAGGGAAAGCAACGGACACCATGACGAGCTTACTC
Oligo donor (Lm221410): 5′ATTTCGTGCTTCTCGCAATCCTTCTCAACGGATGATAGTGCG CGTGTGCG
Lm221410F1: 5′ CCTTTGCGAACTTGAAGGAG
Lm221410R1: 5′ ACGGACAGCACAACCATACA
Lm221410F2: 5′ CTAGGCGATCGAGTCTTTGG
Lm221410R2: 5′ ATCGTGATTCGCTTGTTTCC
Selection of centrin gene deleted clones and single-cell cloning
Leishmania transfections were performed as previously described37. Briefly, 10 µg pLdCNLm221410a&b plasmid DNA was electroporated into 1 × 108 early stationary phase L. major promastigotes. The transfected cells were then selected with G418 (100 µg/ml) for 2 weeks. Once the transfected L. major culture was established, the surviving promastigotes were subjected to three rounds of transfection with the oligonucleotide donor (Lm221410 oligo donor); 10 µl 100 µM single-strand oligonucleotide donor was used per transfection, once every three days. After the third oligonucleotide donor transfection, the Leishmania promastigotes were counted and inoculated into 96 well plates at one promastigote per 100 µl medium per well. The growth of Leishmania cells in 96 well plates was monitored under microscope. After culture for three weeks in 96 well plates, parasites from the relatively slow-growing clones were expanded in 24 well plates. The slow-growing clones were selected since this represents the phenotype for loss of the centrin gene35. The genomic DNA extracted from the slow growth clones were subjected to PCR and DNA sequencing analysis to confirm deletion of the centrin gene.
To remove the pLdCNLm221410a&b plasmid, the centrin gene deleted L. major strain was grown in G418 free medium for several weeks, individual clones were then grown in duplicate plates where one plate contained media without G418 and the duplicate plate contained media with G418. Clones that had lost the plasmid were identified since they lost the ability to survive in the presence of G418.
Genome sequence analysis of LmCen −/−
Complete genome sequencing of two clones from LmCen−/− was determined by MiSeq genome sequencing reaction on an Illumina sequencing instrument at the sequencing core facility at the Center for Biologics Evaluation and Research. LmCen−/− sequence reads were aligned against Leishmania major Friedlin strain reference genome (retrieved from www.tritrypdb.org) using the Burrows-Wheeler Aligner Maximal Exact Match algorithm (BWA-MEM)50. The alignments were converted to BED files using samtools and processed using the bedtools software package51,52. The bedtools coverage command was used with the “-d” option in conjunction with the genomic intervals containing the centrin genes to count the read depth at each position in the coverage of centrin genes shown in Fig. 2a with a 200 bp window. The bedtools coverage command was used in conjunction with gene coordinates extracted from the gff genomic annotation file (retrieved from www.tritrypdb.org53) to compute the percent coverage of each gene as shown in Fig. 2c. Genes with less than 100 percent coverage were manually inspected for a sharp drop-off in coverage (deletion) versus a gradual decline in close proximity to an inverse increase in coverage in a tandem gene (misalignment).
Re-expression of centrin in LmCen −/−
The open reading frame encoding centrin gene was cloned into the SpeI sites of the Leishmania expression plasmid pKSNeo. LmCen−/− parasites were transfected with the plasmid and recombinant parasites were selected using 50 µg/ml G418 to obtain LmCen−/− parasites re-expressing centrin gene termed LmCen−/− Addback (LmCen−/−AB).
Total genomic DNA was isolated from promastigotes with the Wizard genomic DNA purification kit (Promega Biosciences). The DNA (5 µg) was digested with restriction enzyme BglII and the digestion products were separated on 1% agarose gels and transferred to positively charged nitrocellulose membranes. Southern blot analysis of the resolved DNA was performed as described previously using a 32p-labeled L. major centrin ORF nucleotide sequence as a probe39. The DNA fragments were ligated into pCR2.1-Topo vector and the nucleotide sequence of the probe was determined to ensure fidelity. The plasmid containing the correct probe was digested with EcoRI, gel purified and labeled with Random Prime it-II kit using 32p-dCTP (Agilent Technologies).
Mice infection and immunization
Female 6- to 8-wk-old C57BL/6 and BALB/c mice were immunized and/or infected with 1 × 106 total stationary phase LmCen−/− or L. major wildtype (LmWT) parasites by intradermal injection in the left ear in 10 μl PBS. For challenge infections, age-matched naive and seven-week post immunized mice (both C57BL/6 and BALB/c) were challenged in the right ear with 750 metacyclic L. major (WR 2885) wildtype promastigotes intradermally. The numbers of L. major (WR 2885) parasites in the infectious inoculum were determined by a titration analysis revealing that 750 metacyclic parasites cause reproducible pathology in BALB/c mice ear. For leishmanization, mice were infected with 1 × 104 metacyclic promastigotes of L. major Friedlin (FV9) strain by intradermal needle injection in the ear. After 12 weeks of post-infection, healed mice were challenged on the contralateral ear with 1 × 105 metacyclic L. major (FV9) wildtype (LmWT) parasites by needle inoculation.
Lesion size was monitored up to 10 weeks post-challenge by measuring the diameter of the ear lesion using a direct reading Vernier caliper. Parasite burden in the challenged ear and draining lymph node (dLN) was estimated by limiting dilution analysis as previously described37. Briefly, two sheets of ear dermis were separated, deposited in DMEM containing 100 U/ml penicillin, 100 μg/ml streptomycin, and 0.2 mg/ml Liberase CI purified enzyme blend (Roche Diagnostics Corp.), and incubated for 1–2 h at 37 °C. Digested tissue was processed in a tissue homogenizer (Medimachine; Becton Dickinson) and filtered through a 70 μm cell strainer (Falcon Products). Parasite titrations in the ear and dLN were performed by serial dilution (1:1 dilutions) of tissue homogenates in 96-well flat-bottom microtiter plates (Corning, Corning, NY) in M199 cell culture media in duplicate and incubated at 26 °C without CO2 for 7–10 days. The greatest dilution yielding viable parasites was recorded and data are presented the mean parasite dilution ± SEM. For histology, challenged ears were fixed, after 10 weeks of post WT parasite infection, in fixative solutions (10% buffered formalin phosphate solution) and paraffin-embedded sections were stained with hematoxylin and eosin (H&E) (Histoserv Inc.). The stained slides were visualized and photographed using KEYENCE digital microscope.
BALB/c mice were immunized subcutaneously in the footpad with 2 × 108 LmCen−/− parasites of the Friedlin strain or injected with PBS. After 6 weeks both groups were challenged with 104 virulent metacyclics of LV39 L. major parasites intra-dermally in the ear. Ear lesions of vaccinated and non-vaccinated mice (PBS group) challenged with L. major LV39 metacyclic promastigotes were measured at least once a week from week 1 post challenge to week 10 post challenge.
6–8 weeks old female BALB/c, IFN-γ KO (C57BL/6 background) and Rag2 KO (C57BL/6 J (B6(Cg)-Rag2tm1.1Cgn/J) mice were subcutaneously inoculated with 1 × 107 of LmWT (Friedlin V9) or LmCen−/− into the right hind footpad. Following infection, footpad swelling was measured weekly by digital caliper. Parasite burden in infected footpad was measured at 5 weeks after infection in BALB/c, at or at 15 weeks in IFN-γ KO and Rag2 KO mice. 6–8 old weeks female STAT-1 KO (BALB/c background) mice were injected subcutaneously in the footpad with 2 × 108 LmCen−/− parasites of the Friedlin strain or infected with 2 × 108 L. major WT parasites of the Friedlin strain. Footpad swelling of both groups was measured at least once a week from week 1 after injection to week 7. After 7 weeks both groups were sacrificed, and parasite burden was determined. Footpad lesions was excised and then homogenized with a cell strainer in 3 ml of Schneider’s Drosophila medium (Gibco, US) supplemented with 20% heat-inactivated fetal calf serum and Penicillin-Streptomycin (0.1%).
Sand fly infection and transmission of L. major to immunize mice
Female Lutzomyia longipalpis (Jacobina strain, reared at the Laboratory of Malaria and Vector Research, NIAID) sand flies (5–7 days old) were infected by artificial feeding through a chick skin membrane on a suspension of 5 × 106 L. major (WR 2855) procyclic promastigotes/ml of heparinized defibrinated blood containing penicillin and streptomycin. Flies with mature infections were used for transmission54. One day before transmission the sucrose diet was removed. Mice were anesthetized by intraperitoneal injection of 30 μl of ketamine/xylazine (100 mg/ml). Ointment was applied to the eyes to prevent corneal dryness. Ten infected flies were applied to right ears of both LmCen−/− immunized and age-matched naïve C57BL/6 mice through a meshed surface of vials which were held in place by custom made clamps. The flies were allowed to feed on the exposed ear for a period of 2–3 h in the dark at 23 °C and 50% humidity. Following exposure, the number of flies per vial with or without a blood meal was counted to determine the influence of feeding intensity on transmission frequency. Animals were sacrificed 10 weeks post sand fly exposure & organ parasite burden were determined by serial dilution as described above.
Human macrophage infection
Human elutriated monocytes were obtained from NIH blood bank from healthy US blood donors. Only monocytes that tested CMV negative were used in this study. Monocytes were re-suspended at 2 × 105 cells/ml in RPMI medium containing 10% FBS and human macrophage colony-stimulating factor (20 ng/ml, ProSpec), plated in a volume of 0.5 ml in eight-chamber Lab-Tek tissue culture slides (Miles Laboratories) and incubated for 7 days for differentiation into macrophages. The differentiated macrophages were infected with stationary phase LmWT or LmCen−/− promastigotes (10:1 parasite-to-macrophage ratio). After incubation for 6 h at 37 °C in 5% CO2, the free extracellular parasites were removed by RPMI washes and the cultures were incubated in macrophage culture medium for an additional 24 h. The culture medium was removed, and macrophages infected with LmWT or LmCen−/− were stained with Diff-Quik staining reagent. Percentages of infected macrophages were determined by counting a minimum of 100 macrophages per sample under the microscope. Results are shown as mean ± SEM for three independent counts for each infection on days 1–8.
Total RNA was extracted from the ears tissue using a Pure Link RNA Mini kit (Ambion). Total RNA (400 ng) was reverse transcribed into cDNA using random hexamers with a high-capacity cDNA reverse transcription kit (Applied Biosytems). Gene expressions were determined using TaqMan Gene Expression Master Mix and premade TaqMan Gene Expression assays (Applied Biosystems) using a CFX96 Touch real-time system (Bio-Rad, CA) and the data were analyzed with CFX Manager software. The TaqMan Gene Expression Assay ID (Applied Biosystems) of IFN-γ (Mm01168134_m1) and GAPDH (Mm99999915_g1). Expression values were determined by the 2−ΔΔCt method where samples were normalized to GAPDH expression and determined relative to naive sample.
Measurement of cytokine expression from ear-derived CD4+ T cell populations by flow cytometry
To determine the comparative immune response at pre- or 20 h post-L. major WT needle challenge, single-cell suspensions from ear of healed (leishmanized) and LmCen−/− immunized mice were incubated with 1 × 106 T-cell depleted (Miltenyi Biotech) naïve spleen cells (APCs), with 50 µg/ml freeze-thaw L. major antigen (LmFTAg) in flat bottom 48-well plates at 37˚C for 12–14 h. During last 4 h of culture, protein Transport Inhibitor (BD Golgiplug, BD BioSciences) was added to the wells. Cells were then blocked at 4˚C with rat α-mouse CD16/32 (5 µg/ml) from BD BioSciences for 20 min. For surface staining, cells were then stained with α-mouse CD3 AF-700 (BD BioSciences), α-mouse CD4 BV-650 (Biolegend) and α-mouse CD44 FITC (BD BioSciences) or α-mouse CD3 BV421 (BD BioSciences), α-mouse CD4 BV-650 (Biolegend), α-mouse Ly-6C APC-Cy7 (BD BioSciences) and α-mouse CD44 FITC (BD BioSciences) for 30 min (each with 1/300 dilution; 4 ˚C). The cells were then stained with LIVE/DEAD fixable aqua (Invitrogen/Molecular Probes) to stain dead cells. Cells were washed with wash buffer and fixed with the Cytofix/Cytoperm Kit (BD Biosciences) for 20 min (room temperature). Intracellular staining was done with α-mouse IL-2 APC (BD BioSciences), α-mouse IFN-γ PE-Cy7 (Biolegend) and α-mouse TNF-α PerCP-Cy5.5 (Biolegend), for 30 min (each with 1:300 dilution; 4 °C). In some experiments samples were treated with Foxp3 Fixation/Permeabilization Buffer (ebioscience) and then stained with α-mouse T-bet -BV786 (BD Biosciences) according to manufacturer’s instruction. Cells were acquired on Symphony (BD Biosciences) analyzer equipped with 350, 405, 445, 488, 561, 638 and 785 nm LASER lines using DIVA software (v8). Data were analyzed with the FlowJo software version 9.9.6 (BD, San Jose CA). For analysis, first doublets were removed using width parameter; dead cells were excluded based on staining with the Live/Dead Aqua dye. Lymphocytes were identified according to their light-scattering properties. CD4+ T-cells were identified as CD3+ lymphocytes uniquely expressing CD4. Upon further gating intracellular cytokines were measured in Live CD4+CD44HiLy6C+T-bet+ cells. Fluorescence minus one control was used for proper gating of positive events for designated cytokines (Supplementary Fig. 4C, D)
Immunosuppression by dexamethasone injection
To determine the safety of Centrin deficient LmCen−/− parasites in immune-suppressive condition, 4–6 weeks old BALB/c mice were divided into three groups. Group-1 (n = 6) were infected with 1 × 106 stationery phase LmWT parasites and Group-2 (n = 6) and Group-3 (n = 12) animals were immunized with 1 × 106 stationery phase LmCen−/− parasites in a 10 μl volume of PBS through intradermal (into the ear dermis) routes. After 10 weeks of post infection, only Group-3 animals were treated with 2 mg/kg Dexamethasone sodium phosphate (Sigma Aldrich) in PBS by subcutaneous injection three times for one week. Four weeks after this treatment (total 15 weeks post infection); all the groups were sacrificed and evaluated for parasite burden by serial dilution as described above. Development of pathology & lesion size in the ear was assessed at 15 weeks post infection by measuring the diameter of the lesion.
Characterization of centrin deleted parasites isolated from LmCen−/− plus DXM-treated group was done by Polymerase chain reaction. Total Genomic DNA was isolated from the parasites recovered from LmWT and LmCen−/− plus DXM-treated group according to the manufacturer information (DNeasy Blood & Tissue Kit, Qiagen). PCR was performed with L. major centrin gene-specific primer (For-5′-ATGGCTGCGCTGACGGATGAACAGATTCGC-3′; Rev-5′-CTTTCCACGCATCTGCAGCATCACGC-3′) which target the amplification of the 450-bp. A reaction mixture was prepared containing 10 × Buffer (Invitrogen), 0.2 mmol/l each deoxyribonucleotide (Invitrogen), 1 μmol/l each primer, 1.25 units of Taq polymerase (Invitrogen) and 200 ng of DNA samples in a final volume of 50 μl. The PCR conditions were as follows: denaturation at 94 °C for 3 min, followed by 35 cycles of 94 °C for 20 s, 58 °C for 20 s and 68 °C for 35 s with a final extension of 68 °C for 5 min. The amplification reactions were analyzed by 1% agarose gel electrophoresis, followed by ethidium bromide staining and visualization under UV light. DNA from the reference plasmid (PCR 2.1 TOPO) containing centrin gene was used as a positive control.
Statistical analysis of differences between means of groups was determined Student’s t test using Graph Pad Prism 7.0 software. The statistical tests and the significance values are described in the figure legends.
The animal protocol for this study has been approved by the Institutional Animal Care and Use Committee at the Center for Biologics Evaluation and Research, US FDA (ASP 1995#26). The animal protocol is in full accordance with “The guide for the care and use of animals as described in the US Public Health Service policy on Humane Care and Use of Laboratory Animals 2015”. The use of blood components (elutriated monocytes) from the Department of Transfusion Medicine, NIH was approved by the institutional Research Involving Human Subjects Committee (RIHSC#03-120B) at the US FDA. All animal studies at Ohio State University were performed in accordance with NIH guidelines for the humane care and use of animals and were approved by OSU IACUC. Animal experimental procedures performed at the National Institute of Allergy and Infectious Diseases (NIAID) were reviewed by the NIAID Animal Care and Use Committee under animal protocol LMVR4E. The NIAID DIR Animal Care and Use Program complies with the Guide for the Care and Use of Laboratory Animals and with the NIH Office of Animal Care and Use and Animal Research Advisory Committee guidelines. Detailed NIH Animal Research Guidelines can be accessed at https://oma1.od.nih.gov/manualchapters/intramural/3040-2/. Animal experimental procedures performed at Nagasaki University were approved by the Institutional Animal Research Committee of Nagasaki University (No.1606211317 and 1505181227), the Nagasaki University Recombinant DNA Experiments Safety Committee (No. 1403041262 and 1407221278), and performed according to Japanese law for the Humane Treatment and Management of Animals.
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
All relevant data are available in the main text and supplementary information, are available upon reasonable requests to the authors. Source data are provided with this paper.
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Funding was provided from the Global Health Innovative Technology Fund, the Canadian Institutes of Health Research (to GM), intramural funding from CBER, FDA (to H.L.N.), and the Fonds de recherche du Québec – Santé (to P.L.). The findings of this study are an informal communication and represent the authors’ own best judgments. These comments do not bind or obligate the Food and Drug Administration. This research is supported, in part, by the Intramural Reserch Programm of the NIH, National Institute of Allergy and Infectious Diseases, (F.O., I.V.C.A., T.D.V., C.M., J.O., T.D., S.K., J.G.V.).
The FDA is currently a co-owner of two US patents that claim attenuated Leishmania species with the Centrin gene deletion (US7,887,812 and US 8,877,213). All other authors declare they have no competing interests.
Peer review information Nature Communications thanks Nicolas Fasel and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.
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Zhang, WW., Karmakar, S., Gannavaram, S. et al. A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing. Nat Commun 11, 3461 (2020). https://doi.org/10.1038/s41467-020-17154-z
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