Inbred animals were historically chosen for genome analysis to circumvent assembly issues caused by haplotype variation but this resulted in a composite of the two genomes. Here we report a haplotype-aware scaffolding and polishing pipeline which was used to create haplotype-resolved, chromosome-level genome assemblies of Angus (taurine) and Brahman (indicine) cattle subspecies from contigs generated by the trio binning method. These assemblies reveal structural and copy number variants that differentiate the subspecies and that variant detection is sensitive to the specific reference genome chosen. Six genes with immune related functions have additional copies in the indicine compared with taurine lineage and an indicus-specific extra copy of fatty acid desaturase is under positive selection. The haplotyped genomes also enable transcripts to be phased to detect allele-specific expression. This work exemplifies the value of haplotype-resolved genomes to better explore evolutionary and functional variations.
About 10,000 years ago, cattle were domesticated from the aurochs, which ranged across Eurasia and North Africa but are now extinct1. Modern day cattle belong to two subspecies, the humped zebu or indicine breeds (Bos taurus indicus) and the humpless taurine breeds (Bos taurus taurus), which arose from independent domestication events of genetically distinct aurochs populations2.
During the past century, taurine breeds have been intensively selected for production traits, particularly milk and meat yield, and generally have higher fertility than indicine breeds. European taurine breeds, such as Angus, have excellent carcass and meat quality, high fertility, and reach puberty early. These breeds have been imported by farmers around the world to improve or replace less-productive breeds. However, while European taurine animals are well adapted to temperate environments, they do not thrive in hot, humid tropical environments with high disease and parasite challenge.
Indicine breeds originated from the Indus valley and later spread to Africa and across southeast Asia3. Between 1854 and 1926, the four indicine breeds, Ongole, Krishna, Gir, and Gujarat, were imported into the United States and crossed with European taurine cattle to create the Brahman breed. Current US Brahman cattle retain ~10% of their genome of taurine origin4. Brahman have a short, thick, glossy coat that reflects sunlight and loose skin that increases the body surface area exposed for cooling. While Brahman are less productive and have lower fertility than taurine breeds, they have desirable traits, such as heat tolerance, lower susceptibility to parasites such as ticks, and are more disease and drought resistant5.
We previously demonstrated a trio binning approach to assemble haplotypes of diploid individuals at the contig level. The quality of the contigs exceeded those of the best livestock reference genomes6. Here we present chromosome-level taurine (Angus) and indicine (Brahman) cattle genomes from a single crossbred individual that were assembled with haplotype-aware methodology that is less laborious than sequencing haploid clones7. The contiguity and accuracy of the final haplotype-resolved cattle assemblies set a high standard for diploid genomes and enable precise identification of genetic variants, from single-nucleotide polymorphisms (SNPs) to large structural variants (SVs). A further benefit of haplotype-resolved genomes is that they can be used to better interpret allele-specific expression in diploid transcriptome profiles. We identify allele-specific and novel transcripts using PacBio Iso-Seq reads mapped onto the haplotype-resolved genomes. Considering the large differences in production and adaptation traits between taurine and indicine cattle, comparison of genomes between the breeds will contribute to unveiling the mechanisms behind phenotypic differences among cattle including environmental adaptation, which is of substantial scientific and economic interest.
De novo assembly and annotation of Angus and Brahman genomes
The initial creation of haplotigs (haplotype-specific contigs) was presented in the description of the trio binning method implemented in TrioCanu6. Briefly, a male Bos taurus hybrid fetus, from an Angus sire and a Brahman dam, was sequenced to ~136× long-read coverage, and the reads were sorted into parental haplotype bins based on k-mers that are unique to either the paternal or maternal genome, which were identified by short-read sequencing of the parents prior to assembly with TrioCanu. The initial assemblies comprised 1747 Angus haplotigs and 1585 Brahman haplotigs (Table 1). The haplotig N50 was 29.4 and 23.4 Mb for the Angus and Brahman, respectively.
For the present study, additional data were generated for the same hybrid fetus, including ~12× Hi-C reads, ~167× Bionano optical map, and ~84× Illumina paired-end reads (Fig. 1), to provide haplotype-resolved scaffolding and identify assembly errors. Following haplotig assembly, two sets of scaffolds, one based on Hi-C and the other on optical map data, were generated for each haplotype. Three different scaffolding programs (3D-DNA, Proximo, and SALSA2) were evaluated using the Hi-C data (Supplementary Note 1). SALSA2 was found to be the best scaffolder and produced the closest agreement with the latest cattle reference ARS-UCD1.2. The scaffold N50 produced by SALSA2 was larger than that generated by optical map scaffolding, but the latter detected chimeric haplotig more accurately (i.e., a haplotig incorrectly assembled), which resulted in 29 and 36 breaks in the Angus and Brahman haplotigs, respectively (Supplementary Note 2). These chimeric breaks corrected four inter-chromosomal fusions in the initial haplotigs, involving two Brahman chromosomes (13 and 15) and six Angus chromosomes (8, 9, 12, 20, 23, 28).
After validation against a recombination map, gap filling, and error correction, the final assemblies, UOA_Angus_1 and UOA_Brahman_1, had chromosome sizes similar to the current cattle reference, ARS-UCD1.2 (Supplementary Fig. 1). Unlike some of the recent PacBio-based assemblies8, 9, which required an additional polishing step with Illumina short reads to correct the high indel error rates, the haplotype-resolved assemblies only required correction of a very small number of coding sequences, showing that polishing with short reads was unnecessary (Supplementary Note 3).
The Brahman genome was annotated by Ensembl and NCBI, whereas the Angus genome was annotated only by Ensembl (Supplementary Notes 3 and 4). A comparison of annotation features between the Angus, Brahman, and Hereford reference genomes is given in Supplementary Table 1. As the Ensembl pipeline was used to annotate all three cattle genomes, interpretation of results reported here used Ensembl release 96.
Assembly benchmarking and sequence contiguity assessments
The per-base substitution quality values (QVs) for the UOA_Angus_1 and UOA_Brahman_1 reference assemblies were 44.63 and 46.38, respectively (Supplementary Table 2, Supplementary Note 5). The QV represents the phred-scaled probability of an incorrect base substitution in the assembly, hence these QVs indicate that the assemblies are >99.99% accurate at single base level. This is similar to the latest water buffalo assembly UOA_WB_1 (QV 41.96) and surpasses the recent goat ARS1 assembly (QV 34.5) by an order of magnitude. The Angus and Brahman assemblies had ~93% BUSCO completeness score, which demonstrates a high-quality (HQ) assembly of genes (Supplementary Table 3).
The Angus and Brahman assemblies have few gaps compared to most existing mammalian reference assemblies and are comparable to the human GRCh38, the latest Hereford cattle ARS-UCD1.2, and the water buffalo UOA_WB_1 reference genomes (Fig. 2a). For example, the Angus chromosome 24 was assembled without gaps. In terms of contiguity, these cattle reference genomes are comparable to the recent water buffalo UOA_WB_1 assembly9, which is the most contiguous ruminant genome published to date with <1000 contigs (Supplementary Fig. 2), although it is not fully haplotype resolved. While the cattle autosomes showed excellent contiguity, the Brahman X and Angus Y chromosomes were interrupted by 91 and 69 gaps, respectively.
Resolution of longer repeats
The use of long PacBio reads substantially improved repeat resolution compared with the previous cattle assembly UMD3.1.1, which was assembled from Sanger sequences10 (Fig. 2b). Approximately 49% of both Angus and Brahman assemblies consist of repeat elements, which is consistent with other published mammalian assemblies, including human GRCh38, Hereford cattle ARS-UCD1.2, water buffalo UOA_WB_1, and goat ARS1. The two largest repeat families identified were Long Interspersed Nuclear Element (LINE) L1 and LINE/RTE-BovB, which covered ~25% of the chromosomes in both cattle sub-species (Supplementary Fig. 3). Satellite or centromeric repeats (>10 kb) accounted for 21% and 14% of repeats in unplaced scaffolds of Angus and Brahman, respectively. The 7% higher satellite and centromeric repeats in Angus unplaced scaffolds may be due to the presence of the Y chromosome in the Angus haplotype. The combination of the three most frequent repeat families, LINE L1, LINE/RTE-BovB, and satellite/centromeric repeats, covered ~40% of all unplaced bases, and repeat sequences were most frequently responsible for breaking sequence contiguity. The three cattle assemblies constructed using PacBio long reads that resolved repeats >2.5 kb, UOA_Angus_1, UOA_Brahman_1, and ARS-UCD1.2, provide significant improvements in repeat resolution over the previous Sanger-based cattle assembly (UMD3.1.1) (Fig. 2b). In both the Brahman and Angus assemblies, 20 out of 29 of the autosomes contained centromeric repeats within 100 kb of chromosome ends. Vertebrate telomeric repeats (TTAGGG)n were found within 1 Mb of the ends of six Angus and five Brahman chromosomes. This demonstrates that some scaffolds approach chromosome-level assembly.
Discovery of indicus-specific fatty acid desaturase 2
One of the most diverged genomic regions between Brahman and Angus was observed on chromosome 15 (Fig. 3a). A region of ~1.4 Mb has three copies of fatty acid desaturase 2-like genes (FADS2P1) in Brahman, whereas the homologous region in the Angus only has two FADS2P1 genes (Fig. 3b, c). In both Brahman and Angus, the FADS2P1 genes are encoded by 10–12 exons, and the entire region was assembled completely without gaps for both genomes. The region also contains six genes annotated as olfactory receptor-like, with unknown functions, which had differences in their predicted gene models between Brahman and Hereford assemblies. Within the ~1.4 Mb region, there is a high level of sequence divergence for ~200 kb, which is where an extra copy of FADS2P1 lies in Brahman. Searches for FADS2P1 in other ruminant species with HQ genome assemblies revealed that only Brahman has three copies of the gene. The additional FADS2P1 gene is ~53 kb long and is flanked by two other conserved FADS2P1 genes. Searching whole-genome sequencing (WGS) short-read sequences from 38 animals used in this study showed that only Brahman animals had the extra copy, which was not present in any of the taurine individuals. (Supplementary Fig. 4). Considering that the Brahman genome is derived from four indicine breeds, the extra FADS2P1 is likely a Box taurus indicus-specific gene. We used a maximum likelihood-based estimate of ratio of non-synonymous (amino acid changes) to synonymous (silent changes) substitutions as implemented in CODEML to search for positively selected amino acid residues in FADS2P1 and identified 16 significant positively selected sites, 10 of which are located in a small exon 7 of only 60 bp (Fig. 3d, Supplementary Table 4).
SNP and INDEL differences between Brahman and Angus
Mapping short reads from Brahman and Angus to both reference genomes, UOA_Brahman_1 and UOA_Angus_1, revealed that the use of breed-specific reference genomes gave a lower count of all classes of genetic variants. Using WGS short reads from 5 Brahman and 6 Angus individuals, we identified ~24 million Brahman SNPs and ~11 million Angus SNPs, which were annotated using their own reference genome (Table 2, Supplementary Table 6). There were about twice as many INDELs in the Brahman (2,804,421 bp) than the Angus (1,381,548 bp) samples. Lower counts of SNPs, INDELs, and the four classes of SVs (i.e., BND, DEL, DUP, INV) were identified when the appropriate reference genome was used. For example, ~4% fewer SNPs were observed when Brahman individuals were mapped onto the Brahman instead of the Angus reference genome. Additional information on the use of SNPs for the analysis of selective sweeps in cattle is given in Supplementary Note 6.
SV differences between Brahman and Angus
We assessed the structural continuity of our Brahman and Angus genome assemblies against the current cattle reference genome assembly, ARS-UCD1.2, and against WGS datasets from 38 animals representing seven breeds, to ascertain the benefit of using haplotype-resolved assemblies for variant calling. To assess SV differences between Brahman and Angus and the cattle reference genomes, the haplotype-resolved assemblies were aligned to the ARS-UCD1.2 reference (Hereford). This detected insertions, deletions, tandem expansions, tandem contractions, repeat expansions, and repeat contractions11 (Supplementary Fig. 5). Both tandem expansion/contraction and repeat expansion/contraction are repeat-type SVs. Detection of SVs was limited to sizes of 50–10,000 bp, and the total bp affected by SVs in Angus and Brahman were 10.9 and 21.8 Mb. This translates to approximately 0.4% and 0.8% of the Angus and Brahman genomes, respectively. Among the six classes of SVs examined, insertion/deletion types were the most prevalent in both Brahman and Angus genomes compared to ARS-UCD1.2.
We extracted Brahman- and Angus-specific SVs to study their distribution in genic and intergenic regions (Fig. 4a). Brahman-specific insertions/deletions overlapped ~4% of all genes, whereas Angus-specific insertions/deletions overlapped only ~1–2% of genes. Each repeat-type SV overlapped ~1% of genes in Brahman and <1% in Angus. The majority of SVs were found in intergenic regions, and when they overlapped with genes, they were generally localized within introns. Over-representation of Gene Ontology (GO) terms was detected for Angus-specific insertions and tandem contractions and Brahman-specific insertion/deletion SVs at false discovery rate (FDR)-adjusted, Fisher’s exact test, P value < 0.05 (Supplementary Table 7). No over-representation of GO terms was detected for any of the other breed-specific SV types. Interestingly, Brahman-specific insertion SVs have between 3- and 5.7-fold enrichment in phospholipid translocation (GO:0045332), lipid translocation (GO:0034204), lipid transport (GO:0006869), and lipid localization (GO:0010876) GO classes, which suggests that lipid distribution was most impacted by SVs.
Using WGS reads from different datasets, we identified subspecies-specific copy number variations (CNVs) that were masked by the absence or poorer resolution of sequence in the ARS-UCD1.2 reference. The input dataset for these analyses came from ~10× WGS short reads of 38 animals representing 7 cattle breeds. Each set of reads was aligned to all three reference genome assemblies (Hereford, Brahman, and Angus) and processed with SV callers designed to detect read depth differences and paired-end/split-read (PE) discordancy, respectively. The read-depth variation approach included the use of the Vst statistic12, 13 to identify genes with CNV between taurine or indicine lineages using the Brahman, Angus, or ARS-UCD1.2 assemblies (Fig. 4b, Supplementary Fig. 6). The values of Vst varied greatly depending on which reference genome was used for alignment, with taurine-based reference assemblies showing higher variance in copy number between the taurine and indicine lineage datasets (Supplementary Fig. 6) than the Brahman reference (Fig. 4b). Only autosomes were considered. Six CNV genes were found in Brahman, whereas four and eight CNV genes were found in Angus and Hereford, respectively (Fig. 5a–c). Prediction of CNV genes was sensitive to the assembly chosen, e.g., only TMPRSS11D and beta-defensin-like precursor were found to be copy number variable in more than one assembly. Among the 18 CNV genes differentiating indicine from taurine genomes, six unique gene families were identified, which were beta defensin, workshop cluster, trypsin-like serine protease, T cell receptor alpha chain, tachykinin receptor, and interferon-induced very large GTPase, all of which have immune-related functions. All of the CNV genes from these six families showed higher copy number in the indicine cattle lineage regardless of the assembly used. Intersection of liftover CNV regions (CNVRs) called using the Brahman assembly with repetitive elements on ARS-UCD1.2 showed a higher prevalence of CNVs that may have resulted from repeat expansion/contraction in the Brahman reference (1813) than in ARS-UCD1.2 (1238) or the Angus (1164) assemblies. FRC_align statistics showed a higher count of COMPR_PE and STECH_PE events in the Angus assembly (319 and 101, respectively) than the Brahman assembly (263 and 87, respectively), supporting the hypothesis that expansion and contraction of genomic sequence in the Angus assembly is the likely reason for these discrepancies. An olfactory receptor, two long non-coding RNAs and one putative protein, FAM90A12P, also had higher copy numbers among indicine animals. In contrast, ubiquitin-conjugating enzyme E2D3 and two keratin-associated protein 9 genes (KRTAP9-1, KRTAP9-2) had higher copy numbers in the taurine lineage.
We quantified the effects of using different reference assemblies for PE SV discovery. All SV calls of this type were converted into Hereford coordinates to facilitate comparisons. We removed 17, 9, and 18 PE SVs of all types from the Brahman, Angus, and Hereford assemblies that were likely false positives, as they were >1 Mb and did not correspond to aberrant read depth signal to support their SV calls. On average, 0.5% of each cattle genome was covered by CNVRs (Fig. 5d). The majority of CNVRs (at least 76% from each assembly) were found to be unique to one assembly. Among the Brahman CNVRs, only 10% intersected with Angus CNVRs, which suggests mis-assembly in the Hereford reference potentially due to compression of repetitive elements that are more difficult to resolve without phasing haplotypes using the trio binning method.
Allele-specific transcripts in haplotype-resolved genomes
Among the PacBio error corrected Iso-Seq (circular consensus sequence (CCS)) reads pooled from seven tissues of the F1 hybrid fetus, 3,275,676 reads (55%) were classified as full-length non-concatamer (FLNC) reads. After processing with the isoseq3 software, 193,974 full-length, HQ consensus transcripts were generated. We mapped the HQ transcripts to the Brahman reference and obtained 99,329 uniquely mapped transcripts covering 20,940 non-overlapping loci representing 19,403 genes. Of these 99,329 Iso-Seq transcripts, 20,708 (20.8%) had a perfect exon-by-exon match to the reference annotation while 11,359 (11.4%) matched a reference transcript but was missing one or more of the 5’ exons (indicator of 5’ degradation or alternative start site). The majority of the remaining transcripts (59,158, 60%) were novel isoforms of known genes, with the remainder 6.8% of transcripts categorized as intergenic, genomic, or anti-sense that are likely cDNA artifacts. At the gene level, 13,754 of the 19,403 (71%) genes are annotated reference genes. Using the SQANTI2 transcript characterization tool, 83% of the Iso-Seq transcripts fell into coding regions of the Brahman annotation (Fig. 6a). The transcript length distribution ranged from 85 to 11,872 bp, with a median of 3853 bp and a mode of ~4 kb (Fig. 6b).
We validated the IsoPhase SNPs using (1) SNPs called from RNA-Seq data of the brain, liver, lung, muscle, and placenta of the F1 hybrid and (2) Angus SNPs derived from mapping Illumina WGS short reads of the F1 hybrid to the Brahman reference. As the RNA-Seq had greater coverage than Iso-Seq and the SNPs called from genomic DNA included non-transcribed regions, only SNPs that were in positions covered by at least 40 full-length Iso-Seq reads were retained. The concordance of filtered SNPs called from WGS, RNA-Seq, and Iso-Seq is very high (87%) (Fig. 6c). Of the 45,313 SNPs called by IsoPhase, 39,452 (87%) were validated by SNPs from RNA-Seq and WGS, whereas 876 (1.9%) were only validated by RNA-Seq and 2155 (4.7%) were only validated by WGS (Supplementary Note 7). SNP calls that showed inconsistencies could often be explained by lower Iso-Seq coverage, SNPs in homopolymer regions, or alignment artifacts.
Our haplotype-resolved genomes allowed us to explore genes with allelic imbalance in expression. To assess allelic imbalance, the proportion of an allele from each breed was calculated as the normalized count of the Brahman allele divided by the sum of normalized counts of both Brahman and Angus alleles. All tissues showed evidence of imbalance in allelic expression (Shapiro test, P value < 0.01), which was most pronounced for liver, lung, muscle and placenta, whereas brain, heart and kidney were less affected (Fig. 6d). However, as the mammalian brain consists of a wide range of cell types and hence transcriptional complexity, brain tissue was chosen to demonstrate the phasing of transcripts to explore allele-specific expression. The most highly expressed Angus gene with allelic imbalance (ratio of 8 Angus:1 Brahman) in the brain was ARIH2 (also known as TRIAD1), which is known to play a role in protein degradation via Cullin-RING E3 ubiquitin ligases14 (Fig. 6e, f). ARIH2 expression in the liver, lung, muscle, and placenta was also higher from the Angus allele than the Brahman or maternal allele. The HQ transcripts included 23 different transcript isoforms of ARIH2; however, 66% of transcripts for this gene across the 7 tissues were represented by only 3 isoforms. The annotated exons of this gene were in good agreement with the RNA-Seq data (Supplementary Fig. 7).
The most highly expressed Brahman gene with allelic imbalance (ratio of 1 Angus:6 Brahman) in the brain was Calmodulin (CaM), a heat-stable Ca2+-binding protein that mediates the control of numerous physiological processes, including metabolic homeostasis, phospholipid turnover, ion transport, osmotic control, and apoptosis15 (Supplementary Fig. 8). Surprisingly, we also found allelic imbalance (ratio of 1 Angus:16.5 Brahman) in pregnancy-associated glycoprotein 1 (PAG1) with a higher expression of the Brahman allele in the brain and placenta but undetectable in other tissues. This gene was previously thought to be placenta specific and is used as a biomarker for embryo survival16.
Traditional genome assembly approaches collapse haplotypes and therefore do not allow accurate assembly or the study of divergent, heterozygous regions. Here we demonstrate an assembly approach that yielded highly contiguous, haplotype-resolved Brahman and Angus cattle genomes from an F1 hybrid of the two subspecies. Our analyses demonstrated that previous studies4, 17, which mapped indicine sequences onto the taurine reference UMD3.1.1, will have identified loci where the subspecies are fixed for different alleles. Calling SNPs in transcripts from a diploid hybrid with both haplo-genomes decoded provides accurately phased transcripts for studies on the role of allele-specific expression in, e.g., hybrid vigor or heterosis. The phasing of Iso-Seq transcripts in reciprocal crosses will facilitate the exploration of breed-specific effects on parental imprinting, which has been shown in maize18.
We found that the choice of reference assembly had a large impact on SV calling. The observed SV difference between Brahman and Angus is in part due to using Hereford as the reference, which is more closely related to Angus. Ambiguous read alignments, which result from the assignment of reads to incorrect positions on the genome, are a major factor in SV call accuracy19. This is a major concern in the detection of variant sites from alignment of reads to fasta-based reference assemblies. This has prompted the creation of graph-based file formats to improve alignment accuracy20, 21. After converting SVs from each assembly onto the Hereford assembly coordinates and calculating the intersection, we identified 1.3 Mbp of SVs (33% in genic regions) present in the Angus and Brahman assemblies that were not present in the Hereford assembly. This suggests that either the Hereford assembly was not as representative of the true structural variation in these regions or that there were assembly errors in the Angus and Brahman assembly that generated false positive SVs. The latter is less likely given the high accuracy of the Angus and Brahman genomes. Vst estimates for copy number windows incorrectly determined heightened variance between taurine and indicine animals on chromosome 15, suggesting that comparative alignment approaches are prone to a high FDR when used to detect true structural differences between species or subspecies of cattle. If only one reference genome is available for a genus or species, this could present a substantial issue in the interpretation of comparative SV analysis. Conversely, we identified 0.9 Mbp SVs shared between only the Hereford and Angus assembly, which may represent true genomic structural differences between taurine and indicine cattle.
HQ haplotype-specific assemblies facilitate genome-wide comparisons to identify novel variation. The discovery of an indicus-specific, additional copy of FADS2P1, which has been under positive selection, is an example that highlights the benefits of HQ haplotype-specific assemblies. The FADS2P1 gene region in both Brahman and Angus span ~1.4 Mb of sequence, while the two FADS2P1 genes in the water buffalo span ~1 Mb. The orthologous region in goat is ~1 Mb but contains gaps. Taking phylogenetic and information on conservation of synteny together, the most parsimonious explanation is that the extra FADS2P1 was duplicated in the indicine lineage after divergence from taurine cattle. Rapid evolution at the FADS2P1 locus resulted in neofunctionalization of the additional gene in indicine animals, with profound changes seen in the small exon 7.
FADS2 is a pleiotropic gene with known functions in the biosynthesis of unsaturated fatty acids, lipid homeostasis, inflammatory response, and promotion of myocyte growth and cell signaling22,23,24. A non-synonymous SNP in exon 7 of Japanese Black cattle is significantly associated with linoleic acid25 composition. While we do not know the functional significance of positively selected residues in the additional FADS2P1 copy in Brahman, the SNP reported in the Japanese Black shows the importance of exon 7 in FADS2 function. Studies in rats have shown that linoleic acid is an important component of skin ceramides and its deficiency increases water permeability of the skin26. Comparisons between indicine and taurine animals have shown differences in fatty acids27 and types of phosphatidylcholines28. We hypothesize Bos indicus has three copies of FADS2P1 genes to regulate the composition of fatty acids that constitute the cell membranes and could alter water permeability and heat loss from skin.
Brahman cattle may be better adapted to harsher environments because they have slower protein turnover29. Relative to Angus, Brahman have much lower expression of ARIH2 in key metabolic organs, such as the skeletal muscle, and no detectable expression in the liver. ARIH2 promotes ubiquitylation of DCNL1, which is a co-E3 ligase that performs cullin neddylation, a process that regulates one-fifth of ubiquitin-dependent protein turnover14. CNV analysis revealed a decreased number of ubiquitin-conjugating enzyme E2D3 genes in the indicine lineage, which suggests lower protein turnover in indicine animals. While it is still speculative, our findings are consistent with lower protein turnover and the ability of Brahman cattle to withstand stressful conditions.
The analyses of CNV by alignment of short-read sequences from 38 individuals from 7 breeds to the Brahman and Angus genomes revealed that 6 genes with immune-related functions and putative roles in response to disease challenge and external parasites have additional copies in the indicine lineage. Conversely, KRTAP9-2, a gene with significantly altered gene expression following tick infestation30, is expanded in the taurine lineage, which has also been reported in previous CNV studies13, 31. Further studies are needed to elucidate how changes in copy number of KRTAP9-2 affect its expression and its role in tick resistance.
In conclusion, the approach used here is able to create haplotype-resolved genome assemblies that are of higher quality than traditional haplotype-collapsed assemblies. Availability of these HQ assemblies has enabled us to better resolve SVs and identify regions under selection that may be involved in adaptation to the environment. Looking forward, it is clear that HQ haplotype-resolved assemblies together with long-read transcript information will underpin studies on genome function, regulation, and the control of phenotypes.
Bos taurus hybrid
A Bos taurus indicus female (Brahman) was inseminated with semen from a Bos taurus taurus (Angus) bull. The indicus maternal genetic background of the Brahman dam was confirmed by mitochondrial DNA haplotype analysis32. At day 153 post-insemination, dam and conceptus were ethically sacrificed and fetal brain, heart muscle, kidney, liver, lung, skeletal muscle, and placenta (cotyledon) tissue were snap frozen in liquid nitrogen and stored at −80 °C until further use. All animal work was approved by the Animal Ethics Committee of the University of Adelaide (No. S-094-2005).
Genome sequencing and assembly of contigs
DNA was extracted from fetal lung using a salting out method6. Briefly, 100 mg of tissue was ground into powder under liquid nitrogen and then transferred to a tube containing nuclei lysis solution (2 ml buffer of 10 mM Tris-HCl pH 8, 0.4 M NaCl, 2 mM EDTA, 0.2 ml 10% sodium dodecyl sulfate, 0.06 ml 10 mg/ml RNase A). After mixing at 37 °C for 1 h, 0.025 ml of Proteinase K (20 mg/ml) was added to the solution and shaken overnight, and DNA was precipitated by salting out. The dam uterus and bull semen DNA were extracted using standard phenol–chloroform procedures. Twelve SMRT sequencing libraries were made from the fetal DNA using the protocol recommended by the Pacific Biosciences (Procedure P/N 100-286-000-07), with a 15-kb size selection cut-off on a Blue Pippin instrument (Sage Science, Beverley, MA). Nine libraries were sequenced using P6/C4 chemistry on an RSII machine, whereas the remaining three libraries were sequenced on a Sequel machine. Approximately 161 Gb of RSII data and 205 Gb of Sequel data were produced, which gave a total sequence yield of 366 Gb with the mean read length of ~10.4 kb. Assuming a genome size of 2.7 Gb, the raw PacBio data represents ~136× coverage.
Illumina sequencing libraries for both parents (i.e., sire and dam) and F1 fetus were prepared using TruSeq PCR-free preparation kits (Illumina, San Diego, CA). A total of ~55×, ~60×, and ~84× coverage of 150 bp paired-end reads were generated for the sire, dam, and F1 fetus, respectively. In order to assemble phased haplotigs for the F1 Brahman–Angus hybrid, we used the trio binning method introduced by Koren et al.6. Briefly, 21-mers were identified in both sire and dam Illumina reads and 21-mers unique to one or other parent were used to assign the F1 PacBio long reads to the parent of origin. Approximately 1% of the PacBio reads were excluded from the assembly as they lacked parent-of-origin-specific 21-mers, due to their shorter lengths (Supplementary Fig. 9). Long reads that were binned into paternal and maternal groups were assembled separately with TrioCanu v1.6.
Hi-C library preparation and sequencing
A Sau3AI Hi-C library was prepared (Phase Genomics, Seattle, WA) as follows: approximately 200 mg of fetal lung tissue was finely chopped and then cross-linked in Proximo crosslinking solution. The 5’ overhangs after Sau3AI digestion were filled with biotinylated nucleotides, and free blunt ends were ligated. After ligation, crosslinks were reversed and the free DNA was column purified and sonicated to approximately 600 bp peak fragment size (Bioruptor, Diagenode). Hi-C junctions were bound to streptavidin beads and washed to remove unbound DNA. Washed beads were used to prepare sequencing libraries using the HyperPrep Kit (Kapa) following the manufacturer’s protocols. In total, 203 million 2 × 81 bp read pairs were sequenced on NextSeq Illumina platform.
Scaffolding of contigs with Hi-C
All Hi-C reads were mapped to each breed-specific set of haplotigs using BWA v0.7.1533. A haplotype score for a pair was defined as the sum of the percent identity multiplied by match length for each read end (unmapped read ends were assigned a score of 0). Each read pair had two scores, one per haplotype. Pairs with a higher score for one haplotype were considered breed specific and assigned to their respective haplotype. Pairs with a tied score were considered homozygous and assigned to both haplotypes for scaffolding.
Three different Hi-C based scaffolding programs, 3D-DNA34, Proximo (Phase Genomics), and SALSA235, were evaluated for scaffolding contigs. Further detail on the comparison between the scaffolders is given in Supplementary Note 1. Reads were mapped with the Arima mapping pipeline (https://github.com/ArimaGenomics/mapping_pipelinecommit72c81901c671203a86ca4675457004a71d0cd249) and converted to bed format prior to SALSA2 scaffolding (https://github.com/machinegun/SALSAgitcommit863203dd094aaf9b342c35feedde7dabeec37b44), which was run with parameters -c 10000 -e GATC -m yes, for both breed-specific haplotigs.
Bionano DNA isolation and assembly
DNA was extracted from 10 mg kidney tissue from the F1 hybrid using the Bionano Animal Tissue DNA Isolation Kit (P/N 80002) with slight modifications as follows: the frozen tissue was crushed in liquid nitrogen, placed in 2% formaldehyde in Bionano animal tissue homogenization buffer (Document number 30077, Bionano-Prep-Animal-Tissue-DNA-Isolation-Soft-Tissue-Protocol.pdf), and blended with a rotor-stator. The homogenate was passed through a 100-μm nylon filter, fixed on ice for 30 min in 2 ml 100% ethanol, and centrifuged for 5 min at 2000 × g. The resulting pellet was re-suspended in homogenization buffer and added to pre-warmed agarose to make 0.8% agarose plugs. High molecular weight DNA was extracted from the agarose plugs, labeled, stained, and imaged on a Bionano Saphyr system36. Further detail on de novo optical map assembly is given in Supplementary Note 2.
RNA-Seq and Iso-Seq
RNA was extracted from tissue and ground to a fine powder under liquid nitrogen using the Qiagen RNeasy Plus Universal Kit as per the manufacturer’s instructions. RNA quality was assessed using an Agilent TapeStation system and confirmed as RNA integrity number >8 for all samples. Sequencing libraries were prepared with the KAPA Stranded RNA-Seq Library Preparation Kit as per the manufacturer’s protocol and sequenced on an Illumina Next-Seq machine for 100 bp paired-end reads with the target of 50 million reads per sample.
Iso-Seq data were generated from brain, heart muscle, kidney, liver, lung, skeletal muscle, and placenta (cotyledon) tissue. Iso-Seq SMRT bell libraries were created according to the PacBio protocols. Briefly, two size-selected cDNA pools were created, one with an average cDNA size ~3 kb and the second with a cDNA size of ~7 kb. The two pools were then combined for SMRTbell™ Template Preparation. The final average library size was ~5 kb as measured by a bioanalyzer. Each SMRTbell library was loaded onto the Sequel at approximately 50 pM.
Identification and phasing of full-length transcripts
The Iso-Seq data was processed using the isoseq3.1.0 software on the PacBio Bioconda (https://github.com/PacificBiosciences/pbbioconda). The process consists of (1) generating CCS reads, (2) classifying FLNC reads that have the 5‵, 3‵ cDNA primer sequence, and the polyA tail, (3) clustering FLNC reads at the isoform level and generating a draft consensus for each isoform, and (4) polishing each isoform to create HQ, full-length transcript sequences.
The HQ transcript sequences were then mapped to the Brahman reference genome using minimap2 (v2.15-r905) and filtered for alignments that had \(\ge\!\)99% coverage and \(\ge \!\)95% identity. Redundant and degraded transcripts were collapsed using the Cupcake tool (https://github.com/Magdoll/cDNA_Cupcake). SQANTI237 was used to annotate transcripts that belong to seven distinct categories: (i) known isoforms with full-splice match, (ii) known isoforms with incomplete-splice match, (iii) novel isoforms in catalog, (iv) novel isoforms not in catalog, (v) antisense transcripts, (vi) transcripts that overlap with intergenic region, (vii) transcripts that overlap with genic regions.
In order to phase transcripts using the Iso-Seq data, we ran IsoPhase, which is a part of the Cupcake tool, against the Brahman reference. IsoPhase first piles up the FLNC reads of all the isoforms of a gene and calls substitution SNPs using a one-sided Fisher exact test with Bonferroni correction at a P value cut-off of 0.01. It then infers haplotypes based on the phasing information provided by the FLNC reads. The output defines the inferred haplotypes for each transcript and estimates the relative abundance of each allele. We ran IsoPhase using the pooled set of all FLNC reads from all tissues, then later demultiplex them to create an abundance matrix that is specific for each haplotype, per isoform FLNC count for each tissue. To compare the abundance of transcripts across tissues, we normalized the counts by dividing the FLNC counts for each haplotype isoform by the total number of FLNC counts in that tissue, multiplied by a million to obtain the transcript per million number.
Mapping RNA-Seq and WGS reads from the F1 hybrid tissues
Scaffold validation with recombination map
Scaffold contiguity was assessed using a previously published recombination map40. Briefly, the recombination map probe sequences were aligned using BWA MEM v0.7.15 to the scaffolds and the coordinates were arranged in a directed acyclic graph, using a custom script. A contiguity break between consecutive recombination map-ordered probes in the scaffolds was considered an error; however, we tolerated one mismatched probe in a window of three consecutive probes (Hamming distance = 1) to avoid false positive detection due to mapping ambiguity. Despite having Hi-C sequences, some scaffolds that belonged to chromosomes could not be joined together, which necessitated the use of recombination map markers to join and orientate these scaffolds.
Gap filling and polishing
After checking scaffolds with recombination maps40, the Angus and Brahman scaffolds that contained 343 and 369 gaps, respectively, were gap filled with PBJelly41 v15.8.24 using haplotype-specific PacBio subreads. The default parameters of PBJelly were used, except for the support module, where the options captureOnly and spanOnly were used. This step closed 52 and 61 gaps in Angus and Brahman scaffolds, respectively. Two rounds of ArrowGrid (https://github.com/skoren/ArrowGrid) was run to polish the scaffolds to give quality scores.
Assembly evaluation and genome annotation
The assemblies were evaluated with BUSCO v2.0.142 and other metrics that include compression/expansion errors. Annotations were created using the Ensembl gene annotation system43 and the NCBI pipeline. Further detail on the annotation process is given in Supplementary Notes 3 and 4, and for assembly evaluation, detail is given in Supplementary Note 5.
RepeatMasker version open-4.0.7 (http://www.repeatmasker.org) was used to search for repeats in the UOA_Angus_1 and UOA_Brahman_1 assemblies by identifying matches to RepBase (version RepBase23.10.embl)44. Repeats in the current water buffalo assembly (UOA_WB_1) and cattle assembly (UMD3.1.1) were downloaded from the NCBI. Repeats with matches ≤60% identity were filtered out. Centromeric repeats were identified by searching repeats that belonged to the family Satellite/centr in Repbase. We scored a sequence of repeat units as one block and counted the blocks, applying this method systematically throughout for all scaffolds. The vertebrate telomeric repeat, 6-mer TTAGGG, was identified by RepeatMasker. The search for at least 2 consecutive identical TTAGGG repeats within 1000 kb of chromosome ends was done to detect the presence of telomeres.
Gap comparisons and sequence contiguity
To evaluate gaps and sequence contiguity, the Angus and Brahman assemblies were compared to the water buffalo, human, and Hereford cattle assemblies. Only sequences that belong to autosomes and sex chromosomes were retained for analysis, whereas unplaced and mitochondrial sequences were filtered out. The tool seqtk v1.2-r94 (https://github.com/lh3/seqtk) was used to count gaps with similar code implementation as those used for the water buffalo genome9.
SNP and indel calls
Thirty-eight individuals with ~10× WGS short-read Illumina data representing 7 breeds were selected from the USMARC Beef Diversity Panel version 2.9 (MBCDPv2.9)45. The individuals selected for the panel were bulls with minimal pedigree relationships to maximize sampling of diverse alleles suitable for population genetics studies. The number of individuals per breed was as follow: six Angus, five Brahman, six Gelbvieh, six Hereford, five Red Angus, five Shorthorn, and five Simmental. These six taurine breeds were chosen on the basis that they were unlikely to carry B. indicus genetics given their history.
WGS data quality of each individual was checked with FASTQC v0.11.446 and then trimmed with Trim Galore v0.4.247 to a minimum length of 110 bp per read and Phred score of 20. Potential adapters in the sequence reads were removed using AdapterRemoval v2.2.148. Following trimming, the reads were checked with FASTQC again to ensure that only HQ reads were retained. Reads were then mapped to both the Angus and Brahman assemblies separately using BWA v0.7.1533 with the option mem. Samtools v1.849 was used to convert the resulting alignment to sorted bam format. Duplicate reads, which may be due to PCR artifacts, were marked with Picard v2.2.450 MarkDuplicates. The bam files from each individual animal were merged with GATK v439 MergeSamFiles function. Then the following series of GATK functions, AddOrReplaceReadGroups, HaplotypeCaller, CombineGVCFs, and GenotypeGVCFs, were applied to the alignment files to generate a variant call file in VCF v4.2 format. SNPs were filtered with VariantFiltration function using the parameters (QD < 2.0) || (FS > 60.0) || (MQ < 40.0) || (MQRankSum < -12.5) || (ReadPosRankSum < -8.0). Indels were filtered with VariantFiltration function using the parameters (QD < 2.0) || (FS > 200.0) || (ReadPosRankSum < -20.0). Annovar tool51 version dated 2017-07-17 was used to annotate the variants.
SV and copy number variant analyses
WGS short-read data sets from the same 38 animals used for SNP and indel calls were aligned to the UOA_Angus_1, UOA_Brahman_1, and ARS-UCD1.2 with BWA MEM v0.7.1533 and further processed with Samtools v1.949. Read-pair and split-read profile SVs were called with the lumpy-sv v0.2.1352 pipeline, lumpyexpress, using default parameters for each sample. lumpy-sv VCF files were converted to BEDPE format using the vcfToBedpe script included in the lumpy-sv software package. Copy number estimates for genomic segments were calculated from normalized WGS read depth using JaRMS v0.0.13 as previously described53. As JaRMS estimates of genomic copy number are distributed around a value of 1, as the normal diploid copy number count, we multiplied the level estimates from the JaRMS program by two to obtain the adjusted copy number state of genomic regions. JaRMS copy number estimates were used to estimate the population differentiation of taurine and indicine cattle on a per-gene basis using the Vst metric12, 13. A custom script (CalculateVstDifferences.py) was used to automate the calculation of Vst and generation of data tables for plotting. Genes that had a Vst > 0.3, which is equivalent to the top 1% Vst, and a difference in average copy number between groups >3 were considered to have a significant difference in copy number between taurine and indicine populations.
In addition to using short WGS reads from the 38 individuals of 7 breeds to find SVs, the haplotype-resolved Angus and Brahman genomes were aligned with the HQ ARS-UCD1.2 cattle reference to assess SVs. The advantage of aligning to ARS-UCD1.2 was to standardize the SVs specific to each haplotype on a common coordinate system. Contigs obtained by breaking final scaffolds at gap positions from UOA_Angus_1 and UOA_Brahman_1 were aligned using nucmer v454 to the ARS-UCD1.2 assembly to identify the larger structural differences (50–10,000 bp) using Assemblytics11. The nucmer alignment parameters were –maxmatch -t 4 -l 100 -c 500, which was followed by delta-filter with the option -g. Assemblytics parameters followed the default settings, which were “Unique sequence length required: 10000, Maximum variant size: 10000, Minimum variant size: 50.” The overlap of SVs with Ensembl annotation of Hereford cattle ARS-UCD1.2 release 96 were identified with GenomicFeatures and systemPipeR R packages.
Identification, copy number and phylogenetic tree of FADS2P1
All chromosomes from Brahman were aligned to the corresponding Angus chromosomes using the dot plot tool Gepard v1.455. Genomic regions that differed between the two subspecies were isolated for further scrutiny. Of all the regions analyzed, one particular locus on Brahman chromosome 15 at position ~4 Mb covering ~200 kb diverged from the corresponding Angus chromosome. Further analysis revealed an extra copy of FADS2P1 in the Brahman genome. BLASTP56 analysis identified two copies of FADS2P1 in Angus, Hereford, water buffalo, and goat, whereas only Brahman had three copies of this gene. A maximum likelihood tree with 1000 bootstraps was constructed for FADS2P1 homologs using RAxML v857 with substitution model PROTGAMMAAUTO. The conservation of synteny around the FADS2P1 locus was investigated by alignments of Angus to Brahman and Angus to Hereford using nucmer v454 and displayed with Ribbon58.
Positive selection analysis on FADS2P1
The observation of an indicus-specific FADS2P1 residing in a divergent region prompted further investigation into the possibility that the gene is under positive selection. Homologs of FADS2P1 in Brahman, Angus, and water buffalo were subjected to CODEML analysis as implemented in PAML v4.859. Selective pressure acting on a gene can be estimated by the rate ratio (ω) of non-synonymous (amino acid changes) to synonymous (silent changes) substitutions. Detection of ω > 1 is a sign of positive selection, and the site models, namely, M7 and M8 in PAML, which allow ω to vary among sites, were used to detect positive selection. Protein sequences of FADS2P1 homologs were aligned using Muscle60, and the corresponding nucleotides were mapped back onto the amino acid alignment using PAL2NAL61 with gap removal. The tree topology used to run CODEML was a maximum likelihood gene tree calculated from RAxML57. Model M8 was compared with M7 using the likelihood ratio test (LRT) to evaluate if the model with positive selection was favored. More detail on similar positive selection methodology can be found in our study on mammalian glutathione S-transferases62.
R/Bioconductor was used for all statistical analyses. Significance of positively selected sites found in FADS2P1 were evaluated using the LRT, with the test statistic tLR = 2[l(Model 8) − l(Model 7)].
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
The PacBio reads, Hi-C reads, RNA-Seq, Iso-Seq, and Illumina paired-end reads are available in the SRA under BioProject PRJNA432857. The 38 individuals from seven breeds used for variant calls were downloaded from the BioProject PRJNA324822. The assemblies ARS-UCD1.2 (GCF_002263795.1), Bos_taurus_UMD_3.1.1 (GCF_000003055.6), ARS1 (GCF_001704415.1), and UOA_WB_1 (GCF_003121395.1) were downloaded from the NCBI. Intermediary assembly FASTA files and other miscellaneous information are available from the corresponding authors upon request. Annotation files of UOA_Angus_1 and UOA_Brahman_1 are available through Ensembl. Primary accessions number: BioProject: PRJNA432857; GenBank assembly accession for UOA_Angus_1: GCA_003369685.2; and GenBank assembly accession for UOA_Brahman_1: GCA_003369695.2.
Custom scripts can be found at GitHub repository at the following URL: https://github.com/lloydlow/BrahmanAngusAssemblyScripts.
Park, S. D. E. et al. Genome sequencing of the extinct Eurasian wild aurochs, Bos primigenius, illuminates the phylogeography and evolution of cattle. Genome Biol. 16, 234 (2015).
Verdugo, M. P. et al. Ancient cattle genomics, origins, and rapid turnover in the Fertile Crescent. Science 365, 173–176 (2019).
Naik, S. N. Origin and domestication of Zebu cattle (Bos indicus). J. Hum. Evol. 7, 23–30 (1978).
Koufariotis, L. et al. Sequencing the mosaic genome of Brahman cattle identifies historic and recent introgression including polled. Sci. Rep. 8, 17761 (2018).
American Brahman Breeders Association. Available at https://brahman.org (2020).
Koren, S. et al. De novo assembly of haplotype-resolved genomes with trio binning. Nat. Biotechnol. 36, 1174–1182 (2018).
Cao, H. et al. De novo assembly of a haplotype-resolved human genome. Nat. Biotechnol. 33, 617–622 (2015).
Bickhart, D. M. et al. Single-molecule sequencing and chromatin conformation capture enable de novo reference assembly of the domestic goat genome. Nat. Genet. 49, 643–650 (2017).
Low, W. Y. et al. Chromosome-level assembly of the water buffalo genome surpasses human and goat genomes in sequence contiguity. Nat. Commun. 10, 260 (2019).
Zimin, A. V. et al. A whole-genome assembly of the domestic cow, Bos taurus. Genome Biol. 10, R42 (2009).
Nattestad, M. & Schatz, M. C. Assemblytics: a web analytics tool for the detection of variants from an assembly. Bioinformatics 32, 3021–3023 (2016).
Redon, R. et al. Global variation in copy number in the human genome. Nature 444, 444–454 (2006).
Bickhart, D. M. et al. Diversity and population-genetic properties of copy number variations and multicopy genes in cattle. DNA Res. 23, 253–262 (2016).
Kelsall, I. R. et al. Coupled monoubiquitylation of the co-E3 ligase DCNL1 by Ariadne-RBR E3 ubiquitin ligases promotes cullin-RING ligase complex remodeling. J. Biol. Chem. 294, 2651–2664 (2019).
Berchtold, M. W. & Villalobo, A. The many faces of calmodulin in cell proliferation, programmed cell death, autophagy, and cancer. Biochim. Biophys. Acta Mol. Cell Res. 1843, 398–435 (2014).
Lotfan, M. et al. Primary structures of different isoforms of buffalo pregnancy-associated glycoproteins (BuPAGs) during early pregnancy and elucidation of the 3-dimensional structure of the most abundant isoform BuPAG 7. PLoS ONE 13, e0206143 (2018).
Kim, J. et al. The genome landscape of indigenous African cattle. Genome Biol. 18, 34 (2017).
Wang, B. et al. Variant phasing and haplotypic expression from single-molecule long-read sequencing in maize. Commun. Biol. 3, 1–11 (2020).
Abyzov, A., Urban, A. E., Snyder, M. & Gerstein, M. CNVnator: an approach to discover, genotype, and characterize typical and atypical CNVs from family and population genome sequencing. Genome Res. 21, 974–984 (2011).
Kim, D. et al. Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype. Nat. Biotechnol. 37, 907–915 (2019).
Eggertsson, H. P. et al. Graphtyper enables population-scale genotyping using pangenome graphs. Nat. Genet. 49, 1654–1660 (2017).
Gol, S. et al. polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs. Sci. Rep. 8, 14336 (2018).
Markworth, J. F. et al. Arachidonic acid supplementation modulates blood and skeletal muscle lipid profile with no effect on basal inflammation in resistance exercise trained men. Prostaglandins Leukot. Essent. Fat. Acids 128, 74–86 (2018).
Markworth, J. F. & Cameron-Smith, D. Arachidonic acid supplementation enhances in vitro skeletal muscle cell growth via a COX-2-dependent pathway. Am. J. Physiol. Physiol. 304, C56–C67 (2013).
Takahashi, H. et al. Association of bovine fatty acid desaturase 2 gene single-nucleotide polymorphisms with intramuscular fatty acid composition in Japanese Black steers. Open J. Anim. Sci. 06, 105–115 (2016).
Hansen, H. S. & Jensen, B. Essential function of linoleic acid esterified in acylglucosylceramide and acylceramide in maintaining the epidermal water permeability barrier. Evidence from feeding studies with oleate, linoleate, arachidonate, columbinate and α-linolenate. Biochim. Biophys. Acta Lipids Lipid Metab. 834, 357–363 (1985).
Bressan, M. C. et al. Genotype x environment interactions for fatty acid profiles in Bos indicus and Bos taurus finished on pasture or grain. J. Anim. Sci. 89, 221–232 (2011).
Sudano, M. J. et al. Phosphatidylcholine and sphingomyelin profiles vary in Bos taurus indicus and Bos taurus taurus in vitro- and in vivo-produced blastocysts. Biol. Reprod. 87, 130 (2012).
Sainz, R. D., Barioni, L. G., Paulino, P. V. R., S.C.Valadares & Filho, J. W. Growth Patterns of Nellore vs. British Beef Cattle Breeds Assessed using a Dynamic, Mechanistic Model of Cattle Growth and Composition (eds Kebreab, E., Dijkstra, J., Bannink, A., Gerrits, W. J. J. & France, J.) Ch. 16 (CAB eBooks, 2006).
Wang, Y. H. et al. Gene expression profiling of Hereford Shorthorn cattle following challenge with Boophilus microplus tick larvae. Aust. J. Exp. Agric. 47, 1397 (2007).
Bickhart, D. M. et al. Copy number variation of individual cattle genomes using next-generation sequencing. Genome Res. 22, 778–90 (2012).
Hiendleder, S., Lewalski, H. & Janke, A. Complete mitochondrial genomes of Bos taurus and Bos indicus provide new insights into intra-species variation, taxonomy and domestication. Cytogenet. Genome Res. 120, 150–156 (2008).
Li, H. & Durbin, R. Fast and accurate short read alignment with Burrows–Wheeler transform. Bioinformatics 25, 1754–1760 (2009).
Dudchenko, O. et al. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds. Science 356, 92–95 (2017).
Ghurye, J. et al. Integrating Hi-C links with assembly graphs for chromosome-scale assembly. PLOS Comput. Biol. 15, e1007273 (2019).
Formenti, G. et al. SMRT long reads and direct label and stain optical maps allow the generation of a high-quality genome assembly for the European barn swallow (Hirundo rustica rustica). Gigascience 8, (2019).
Tardaguila, M. et al. SQANTI: extensive characterization of long read transcript sequences for quality control in full-length transcriptome identification and quantification. Preprint at https://doi.org/10.1101/118083 (2017).
Kim, D., Langmead, B. & Salzberg, S. L. HISAT: a fast spliced aligner with low memory requirements. Nat. Methods 12, 357–360 (2015).
DePristo, M. A. et al. A framework for variation discovery and genotyping using next-generation DNA sequencing data. Nat. Genet. 43, 491–498 (2011).
Ma, L. et al. Cattle sex-specific recombination and genetic control from a large pedigree analysis. PLOS Genet. 11, e1005387 (2015).
English, A. C. et al. Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology. PLoS ONE 7, e47768 (2012).
Simão, F. A., Waterhouse, R. M., Ioannidis, P., Kriventseva, E. V. & Zdobnov, E. M. BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs. Bioinformatics 31, 3210–2 (2015).
Aken, B. L. et al. The Ensembl gene annotation system. Database 2016, baw093 (2016).
Bao, W., Kojima, K. K. & Kohany, O. Repbase Update, a database of repetitive elements in eukaryotic genomes. Mob. DNA 6, 11 (2015).
Heaton, M. P. et al. Using diverse U.S. beef cattle genomes to identify missense mutations in EPAS1, a gene associated with high-altitude pulmonary hypertension. F1000Research 5, 2003 (2016).
Andrews, S. FastQC: a quality control tool for high throughput sequence data. https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ (2010).
Krueger, F. Trim Galore!: a wrapper tool around Cutadapt and FastQC to consistently apply quality and adapter trimming to FastQ files, with some extra functionality for MspI-digested RRBS-type (Reduced Representation Bisufite-Seq) libraries. https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/ (2015).
Schubert, M., Lindgreen, S. & Orlando, L. AdapterRemoval v2: rapid adapter trimming, identification, and read merging. BMC Res. Notes 9, 88 (2016).
Li, H. et al. The sequence alignment/map format and SAMtools. Bioinformatics 25, 2078–2079 (2009).
Broad Institute. Picard tools. Broad Institute, GitHub repository. http://broadinstitute.github.io/picard/ (2020).
Wang, K., Li, M. & Hakonarson, H. ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data. Nucleic Acids Res. 38, e164 (2010).
Layer, R. M., Chiang, C., Quinlan, A. R. & Hall, I. M. LUMPY: a probabilistic framework for structural variant discovery. Genome Biol. 15, R84 (2014).
Oldeschulte, D. L. et al. Annotated draft genome assemblies for the Northern Bobwhite (Colinus virginianus) and the scaled quail (Callipepla squamata) reveal disparate estimates of modern genome diversity and historic effective population size. G3 (Bethesda) 7, 3047–3058 (2017).
Marçais, G. et al. MUMmer4: a fast and versatile genome alignment system. PLOS Comput. Biol. 14, e1005944 (2018).
Krumsiek, J., Arnold, R. & Rattei, T. Gepard: a rapid and sensitive tool for creating dotplots on genome scale. Bioinformatics 23, 1026–1028 (2007).
Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. Basic local alignment search tool. J. Mol. Biol. 215, 403–410 (1990).
Stamatakis, A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30, 1312–1313 (2014).
Nattestad, M., Chin, C.-S. & Schatz, M. C. Ribbon: visualizing complex genome alignments and structural variation. Preprint at https://doi.org/10.1101/082123 (2016).
Yang, Z. PAML 4: phylogenetic analysis by maximum likelihood. Mol. Biol. Evol. 24, 1586–1591 (2007).
Edgar, R. C. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32, 1792–7 (2004).
Suyama, M., Torrents, D. & Bork, P. PAL2NAL: robust conversion of protein sequence alignments into the corresponding codon alignments. Nucleic Acids Res. 34, 609–612 (2006).
Tan, H. M. & Low, W. Y. Rapid birth-death evolution and positive selection in detoxification-type glutathione S-transferases in mammals. PLoS ONE 13, e0209336 (2018).
This work was supported with supercomputing resources provided by the Phoenix HPC service at the University of Adelaide. The work was part funded by the JS Davies bequest to the University of Adelaide. We thank Bob Lee, Kristen Kuhn, Kelsey McClure, and William Thompson for technical assistance. We acknowledge funding from the Wellcome Trust (108749/Z/15/Z), the Biotechnology and Biological Sciences Research Council (BB/M011615/1 and BB/S020152/1) and the European Molecular Biology Laboratory. The work was supported in part by funds from USDA-ARS Project Number 3040-31320-012-00D. The use of trade names or commercial products in this manuscript is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity employer and provider. A.R., S.B.K., and A.M.P. were supported by the Intramural Research Program of the National Human Genome Research Institute, US National Institutes of Health. A.R. was also supported by the Korean Visiting Scientist Training Award (KVSTA) through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare (HI17C2098). The work of F.T.-N. was supported by the Intramural Research Program of the National Library of Medicine, National Institutes of Health. This work utilized the computational resources of the NIH HPC Biowulf cluster (https://hpc.nih.gov).
S.B.K., Z.N.K., and E.T. are employees of Pacific Biosciences. A.R.H., J.L., and A.W.C.P. are employees of BioNano Genomics. J.G. is an employee of Dovetail Genomics. The remaining authors declare no competing interests.
Peer review information Nature Communications thanks Fritz Sedlazeck and the other anonymous reviewer(s) for their contribution to the peer review of this work.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Low, W.Y., Tearle, R., Liu, R. et al. Haplotype-resolved genomes provide insights into structural variation and gene content in Angus and Brahman cattle. Nat Commun 11, 2071 (2020). https://doi.org/10.1038/s41467-020-15848-y
Investigating the accuracy of imputing autosomal variants in Nellore cattle using the ARS-UCD1.2 assembly of the bovine genome
BMC Genomics (2020)
BMC Genomics (2020)
Nature Communications (2020)