Inferences of the interactions between species’ ecological niches and spatial distribution have been historically based on simple metrics such as low-resolution dietary breadth and range size, which might have impeded the identification of meaningful links between niche features and spatial patterns. We analysed the relationship between dietary niche breadth and spatial distribution features of European bats, by combining continent-wide DNA metabarcoding of faecal samples with species distribution modelling. Our results show that while range size is not correlated with dietary features of bats, the homogeneity of the spatial distribution of species exhibits a strong correlation with dietary breadth. We also found that dietary breadth is correlated with bats’ hunting flexibility. However, these two patterns only stand when the phylogenetic relations between prey are accounted for when measuring dietary breadth. Our results suggest that the capacity to exploit different prey types enables species to thrive in more distinct environments and therefore exhibit more homogeneous distributions within their ranges.
The study of the relationship between the ecological niche breadth and spatial distribution of species has been a core topic in ecology, since Hutchinson’s initial conceptualisation of the ecological niche1,2,3. Ecological niche breadth measures the degree of specialisation or generalisation of the resources species use (Eltonian definition of ecological niche) or the conditions which they inhabit (Grinnellian definition)4,5. Different domains of the ecological niche, such as climatic tolerance, habitat breadth and dietary breadth, have been shown to be positively associated with species’ geographical range sizes3, which is the feature historically employed to characterise the spatial distribution of species2. The relationship between ecological niche breadth and range size is based on the idea that species that use a greater array of resources, and thrive under a wider variety of conditions, should become more widespread2,6. However, the effect size of the correlations gradually decreases when transitioning from Grinnellian (e.g. climatic tolerance) to Eltonian (e.g. diet) niche features3. In fact, a meta-analysis of 20 independent studies showed that dietary niche breadth is only positively related to range size in arthropods, but not in vertebrates3.
The lack of a consistent link between dietary niche breadth and range size in vertebrates could be due to their broader dietary niche breadth in comparison to that of the more thoroughly studied phytophagous arthropods, which typically exhibit a larger degree of resource specialisation7,8. In addition, vertebrates often exhibit mismatches between different niche components; they can be generalists for dietary resources, but specialists for some other resources, such as roosts9,10. These discrepancies could lead to discordance between the given Eltonian features and range size. Finally, range size is also known to be largely dependent on the historic legacy of speciation, Quaternary climatic changes, and the dispersal capacity of species11.
Besides range size, other spatial features might also be related to dietary niche breadth. The homogeneity of the spatial distribution, i.e. how evenly animals are distributed within their geographic range12, is one of them. It has been hypothesised that a generalist species (e.g. wide dietary niche) will be able to find useful resources in more different environments than a specialist (e.g. narrow dietary niche), so their spatial distribution should become more homogeneous13. While the hypothesis that links dietary niche breadth with range size has been thoroughly studied3, the hypothesis that relates dietary niche breadth with distribution homogeneity has received far less attention13.
We argue that because traditionally employed metrics are excessively simplified, they might be unable to reveal ecologically meaningful relationships between dietary niche features and broad-scale spatial patterns in vertebrates. However, current analytical tools might provide the required amplitude and resolution to unravel more complex links. High-throughput DNA sequencing-based (DNA metabarcoding) and species distribution modelling (SDM) tools now enable more nuanced analysis of dietary and spatial patterns. DNA metabarcoding allows comprehensive analysis of dietary variation, by considering different components of dietary diversity, such as richness (how many prey types are consumed), evenness (the balance of the relative consumption of each prey), and regularity (the degree of similarity across consumed prey)14,15. SDMs predict how presence probability is distributed across the geographic range of a species, hence enabling the estimation of homogeneity of the spatial distribution.
In the present study, we contrast broad-scale dietary and spatial features of a vertebrate system, namely a European bat assemblage, with the aim of gaining further insights into the relationship between the dietary niche breadth and spatial features of vertebrates. Bats provide an excellent case study for understanding the relationship between dietary and spatial patterns. The dietary breadth of European insectivorous bats varies considerably, from specialists on certain arthropods—usually moths—to generalists that consume a wide range of taxa16,17. Similarly, the spatial distribution of species also exhibits marked differences18. Specifically, we hypothesise that the predicted geographic distributions of species would be more homogeneous in bats with broader dietary niches.
We characterise the dietary niche of seven bat species by analysing over 400 individual faecal samples, collected at 40 locations scattered across the European continent. Faeces of each individual bat are independently analysed through DNA metabarcoding and high-throughput sequencing, using two complementary primer sets and three replicates per primer. The level of dietary specialisation is measured using the statistical framework recently developed around Hill numbers19, by considering different components of dietary diversity: richness (dR), richness + evenness (dRE) and richness + evenness + regularity (dRER), and applying both incidence- and abundance-based approaches to quantify diet. In parallel, we generate species distribution models of the bat species, using high-quality presence records and an ensemble approach that combine different modelling algorithms. Spatial features derived from these models, such as potential range size and degree of homogeneity of the predicted suitable distributions, are also characterised based on Hill numbers applied to spatial data. The contrast between different components of dietary diversity and spatial patterns enable us to unveil relationships between dietary niche breadth and broad-scale spatial features, and test whether the hypothesis that correlates dietary niche breadth with distribution homogeneity stands in the studied European bat assemblage.
The dietary niche of European bats is dominated by Lepidoptera and Diptera
After applying all quality filters, the dataset included dietary information of 355 individual bats belonging to seven species (DNA sequencing details in Supplementary Table 4). Using two primer sets, we detected over 3000 different prey taxa belonging to 29 arthropod orders (Fig. 1a), though the diet of European bats was dominated by Lepidoptera and Diptera (Fig. 1b). This pattern was consistent for data generated with both primer sets (Supplementary Fig. 3), as well as both incidence- and abundance-based approaches employed to quantify dietary profiles (Supplementary Fig. 4). The reliability of the results was further confirmed through in silico taxonomic amplification bias analyses, which showed that Epp primers do not exhibit the bias towards Lepidoptera and Diptera (Supplementary Fig. 5) that is known to affect the Zeale primers20,21. Our results complement the existing broad-scale molecular dietary data of Miniopterus schreibersii17, and provide geographically widespread molecular insights into the dietary ecology of Myotis daubentonii, Myotis myotis, Myotis emarginatus, Myotis capaccinii, Rhinolophus euryale and Rhinolophus ferrumequinum, which to our knowledge had only been studied at local scales previously16,22,23,24,25.
Dietary niche breadth differences depend on the components of diversity accounted for
Dietary niche breadth measures (Fig. 1c), and species’ dietary generalisation/specialisation ordination plots derived from them (Fig. 1d), were different depending on the components of diversity considered for quantifying dietary niches. Such a dependency on the considered diversity components has also been reported in other systems26,27, and it has been attributed to the fact that each diversity component might be driven by different ecological forces28. For instance, the dietary niches of M. schreibersii and R. euryale showed similar dRE values, yet the contribution of richness and relative evenness components differed. The dietary richness of M. schreibersii was almost 40% larger than R. euryale’s, while the evenness factor of R. euryale was almost 30% higher than that of M. schreibersii. These differences could be explained by (i) the larger home range of M. schreibersii compared to R. euryale29,30, which might expose the former to more prey species—thus increasing dietary richness, and (ii) the higher incidence of a few locally abundant pest moth species in the diet of M. schreibersii than in that of R. euryale17,22—yielding lower relative evenness. As reported for other systems31, such dietary niche differences would be overlooked if the niche breadth analyses were limited to a single diversity metric.
Broad-scale spatial homogeneity does not correlate with range size
Similar to dietary niche features, spatial features of species can also be measured through different metrics. To understand the relationship between the different spatial properties, we calculated the most commonly employed feature, namely range size, using IUCN cartography32, hereafter referred to as recognised range size. We contrasted it with two other spatial features predicted through species’ distribution modelling (Fig. 2a–g, Supplementary Table 5), namely potential range size and spatial homogeneity of the distribution. As expected, species’ recognised range size within the studied region (Europe) was positively correlated with potential range size (Fig. 2h; linear model (LM): F = 17.36, df = 1,5, r2 = 0.77, p value = 0.008). In contrast, spatial homogeneity exhibited no linear relation with range size (LM: F = 0.91, df = 1,5, r2 = 0.15, p value = 0.382). This is best illustrated by the fact that the two of the species with the highest homogeneity exhibited the largest (M. daubentonii) and smallest (M. capaccinii) recognised and potential range sizes in Europe (Fig. 2i).
Dietary niche breadth correlates with spatial homogeneity
The contrast between the different dietary niche breadth metrics and recognised range size was not significant (Fig. 2j, Supplementary Fig. 6). Similarly, dietary niche breadth was not correlated with spatial homogeneity when only richness and evenness components were considered (Fig. 2k, Supplementary Fig. 7; LM: F = 0.03, df = 1,5, r2 = 0.007, p value = 0.85). However, we found that the dietary niche breadth measure that accounts for all diversity components considered (dRER) was positively correlated with broad-scale spatial homogeneity of species (Fig. 2i; linear mixed model (LMM): t = 4.95, df = 5, r2marginal = 0.72, r2conditional = 0.92, p value = 0.004). This pattern was consistent for both primer sets, the overall averaged dataset, as well as the two analytical approaches (i.e. abundance- and incidence-based) employed (Supplementary Fig. 8). According to our results, the species that consume a wider variety of distinct prey are not those that have larger range sizes, but rather those that exhibit more homogeneous spatial distribution within their ranges.
Species’ dietary niche breadth is mainly driven by prey turnover across individuals
We decomposed dietary diversity into alpha (individual bats), beta and gamma (bat species) components to gain insights into the sources of variation across species, because generalist species can be heterogeneous collections (high beta diversity) of specialised (low alpha diversity) individuals33. It has been further proposed that species with wider niches should exhibit larger levels of individual specialisation coupled with greater among-individual variation in resource use, the so-called niche variation hypothesis34,35. Our cross-sectional study design did not enable us to ascertain the dietary specialisation level of each analysed individual, but provided some insights into the structure of dietary diversity. We did not observe a negative linear relationship between dietary niche breadth and individual niche breadth (Fig. 3a; LM: F = 0.10, df = 1,5, r2 = 0.02, p value = 0.76). However, dietary niche breadth (dRER) did exhibit a strong linear relationship with prey turnover across individual bats (Fig. 3b; LM: F = 22.94, df = 1,5, r2 = 0.82, p value = 0.004), showing that dietary niche breadth is shaped by inter-individual variation of diet composition, as the niche variation hypothesis predicts. Prey turnover also exhibited a borderline positive linear trend with distribution homogeneity (Fig. 3c; LM: F = 5.09, df = 1,5, r2 = 0.50, p value = 0.07). This observation suggests that species that exhibit more homogeneous distributions across their ranges exhibit broader dietary niche breadths, probably because they are able to exploit different types of dietary resources in different environments.
Dietary niche breadth is associated with hunting plasticity
Lastly, we analysed the relation of other behavioural traits (hunting plasticity, habitat use diversity and roosting plasticity) with dietary breadth, range size and spatial homogeneity, to explore the links of other niche axes with dietary and spatial features. We found no significant correlation between any of the analysed traits and spatial features, both range size and distribution homogeneity (Supplementary Fig. 9). However, we found that hunting plasticity is positively related with the dietary niche breadth of predator species (Fig. 3d; LMM: t = 3.135, df = 5, r2marginal = 0.56, r2conditional = 0.96, p value = 0.026). This suggests that the ability to use a more diverse range of hunting strategies, such as capturing prey from the ground, foliage or water surface, in addition to hunting flying prey36, also contributes to an obvious broadening of the functional spectrum of captured prey. This increased flexibility could render animals less sensitive to environmental heterogeneity, and thus also contribute to the homogenisation of their spatial distributions.
We combined high-resolution broad-scale dietary (DNA metabarcoding) and spatial (species distribution modelling) characterisation to relate diet with broad-scale spatial patterns. While dietary niche breadth has been previously shown to predict geographic range sizes in certain taxa3, dietary niche breadth has not previously been associated with other broad-scale spatial features of species. The ecological niches of bats are complex arrays of different resources, and spatial features are most probably also affected by other niche axes9,10. However, the potential noise such confounding factors could have introduced was not strong enough to mask the correlations our data unveiled between dietary breadth and homogeneity of spatial distributions. In addition, after analysing three other Eltonian niche features of bats, only dietary breadth showed significant correlations with spatial features. It is remarkable that a single snapshot of the diet of individual bats was enough to recover a clear link between dietary and spatial features, as niche patterns are not always coupled at individual and population levels37. Our results therefore depict diet as one of the most relevant ecological features associated with broad-scale spatial distributions of European bats.
This observation challenges the traditional view on the relevance of Eltonian features at different scales. The ecological significance of the functional traits of species changes across geographic scales, and it is considered that fine-scale features such as resource use (e.g. diet) are “averaged out”, and instead, non-interactive variables (e.g. climatic tolerance) determine the broad aspects of distributions of species38. However, it has been recently shown that, although strongest at fine grains, interactive variables can remain important even at larger spatial scales39. For instance, dietary features have been shown to contribute to shaping broad-scale species distributions in parrots40. Our results also show that dietary features are linked to broad-scale spatial features of bats. Our data associate dietary diversification with homogeneity of spatial distribution, through an increased prey turnover across individuals. We also show that dietary breadth is correlated with an increased capacity to employ different hunting strategies. These two observations suggest that the pattern is the result of the combination of the capacity to exploit different prey resources and being exposed to different available prey. The ability to modulate the hunting strategy enables bats exploiting structurally distinct environments, thus eliciting a homogeneous dispersion over the landscape.
It is noteworthy that all the patterns we found in this study were recovered using dietary and spatial distribution metrics that are largely overlooked in the literature. Our dietary breadth metrics achieved explanatory power only when all three components of diversity were considered. No diversity component alone was correlated with spatial features or other axes of the ecological niche of bats, which highlights the importance of accounting for relative evenness and regularity of prey when measuring dietary niches15,27. Similarly, the spatial feature that dominates the literature, namely range size, showed no link with dietary features, while a far less studied feature such as distribution homogeneity did. Our results therefore suggest that exploring ecological patterns beyond simple traditional metrics might unveil associations that otherwise would remain hidden.
Further work will be necessary to ascertain whether the observed patterns are limited to the bat assemblage studied, or can be extended to other taxa and geographic regions. Given the uneven impact climate change is having on species with distinct ecological niches—comparatively favouring generalists over specialists41,42—understanding the complex relationships between ecological traits and spatial distribution patterns is of paramount importance for predicting impacts over species with different ecological features. In that regard, integrative approaches that leverage tools and knowledge developed in different fields of biological sciences, such as the one showcased in this study, will be critical for unravelling relevant ecological patterns in the intersection between Eltonian and Grinnellian niches.
Sample collection and dietary data generation
We collected droppings from 402 individual bats captured in 40 locations distributed across Europe (Supplementary Table 1), in June−October of 2015–2017. The droppings belonged to seven species: Miniopterus schreibersii (MSc), Myotis capaccinii (MCa), Myotis daubentonii (MDa), Myotis emarginatus (MEm), Myotis myotis (MMy), Rhinolophus euryale (REu) and Rhinolophus ferrumequinum (RFe). Using a randomised setup, DNA was extracted from all individual samples using the PowerSoil® DNA Isolation Kit (MoBio, CA, USA) principally following the manufacturer’s protocol (2016 version), but with some modifications (see Supplementary Information). Extracts were amplified in three replicates using two primer pairs, referred to as Zeale (ZBJ‐ArtF1c: AGATATTGGAACWTTATATTTTATTTTTGG; ZBJ‐ArtR2c: WACTAATCAATTWCCAAATCCTCC)43 and Epp (Coleop_16Sc: TGCAAAGGTAGCATAATMATTAG; Coleop_16Sd: TCCATAGGGTCTTCTCGTC)44, after determining optimal PCR conditions (shown in Supplementary Tables 2 and 3) through quantitative PCR (qPCR) screening. Both primers were 5′ nucleotide tagged to yield a set of unique forward and 60 unique reverse primers. Tags were 7 nucleotides in length and there were 2–3 nucleotide mismatches between tags. Each PCR amplification was carried out with matching tags (e.g. F1-R1, F2-R2, etc.) to ensure tag jumps would not result in false assignments of sequences to samples. The three PCR replicates from each sample were carried out with different tag combinations to minimise the possible effect of tag bias. Each PCR round contained 96 samples, including 90 bat dropping samples, four extraction blanks and two PCR blanks. All PCR mixes were set up in a dedicated pre-PCR laboratory to minimise the risk of contamination. Amplicons were bead-purified, pooled and built into libraries using a single-tube library preparation method45 modified for amplicon samples. Purified libraries were analysed in an Agilent Bioanalyzer and combined into sequencing pools using equimolar ratios. Library pools were spiked with 15% PhiX before sequencing them in multiple Illumina MiSeq flow cells using 250PE chemistry and aiming 35,000 reads per PCR replicate per sample.
Dietary metabarcoding bioinformatics procedures
The paired end reads in each sequencing library were merged and quality filtered using AdapterRemoval 2.1.7 46 and the reads within each library were sorted according to primer and tag sequences using DAMe47. To ensure maximum DNA sequence reliability, only high-quality sequences that appeared in at least two of the three PCR replicates were retained, and sequences identical to those detected in the extraction and library blanks of the corresponding processing batch of each sample were removed. Appropriate sampling depth per sample was ensured by discarding samples with insufficient sequencing depth as assessed by rarefaction curves and curvature indexes. DNA sequences were clustered into operational taxonomic units (OTUs) based on 98% identity following Alberdi et al.48. Samples with less than 5000 sequences were removed, and OTUs with a representation below 0.02% in each sample were removed for their probability of being false positives derived from PCR and sequencing errors. Taxonomy was assigned by aligning the OTU representative sequences to the Genbank nt49–and in the case of Zeale also BOLD50–databases. Bayesian OTU phylogenetic trees were generated using BEAST2 51 after aligning the OTU representative sequences using CLUSTAL Omega52. All the analyses were performed with a minimal Markov chain Monte Carlo (MCMC) chain length of 108 iterations, sampling trees every 1000. Each Bayesian run was repeated, and convergence of the MCMC chains and sample size was checked using TRACER 1.6.0. To account for the phylogenetic uncertainty of the reconstructed trees, 50 trees were randomly selected from the last 5% (5000) of the trees sampled across the MCMC, as detailed by Alberdi et al.15. Full details of the bioinformatics methodologies are reported in the Supplementary Information and Supplementary Code 1.
Dietary diversity analyses
Diversity analyses were carried out using the R package hilldiv53 based on abundance-based Hill numbers54,55. The Hill numbers framework enables (i) the relative weight given to abundant and rare OTUs to be modulated through a single parameter, namely the order of diversity q54, and (ii) the similarity level across OTUs to be overlooked or accounted for when computing diversity. Although functional diversities can be computed using Hill numbers56, given the infeasibility of gathering ecological trait information of thousands of prey items, OTU phylogenies were employed as proxies of ecological resemblance across OTU27. Hence, dR (richness) was computed as the neutral Hill number of order of diversity q = 0; dRE (richness + evenness) was computed as the neutral Hill number of order of diversity q = 1–i.e. Shannon diversity–and dRER (richness + evenness + regularity) was computed as the phylogenetic Hill number of order of diversity q = 1. Phylogenetic Hill numbers were computed based on Bayesian phylogenies generated from metabarcoding DNA sequences, and the analyses accounted for the phylogenetic uncertainty of generated trees, as detailed in Supplementary Information. Prey turnover was measured by means of Jaccard‐type turnover using hilldiv, based on the beta diversity value derived from the multiplicative hierarchical partitioning of the species’ dietary diversity into alpha (individuals) and beta (across individuals) components. The Jaccard‐type turnover quantifies the normalised prey turnover rate (across individuals) with respect to the whole system (species)57.
Spatial distribution analyses
Recognised range sizes were calculated from distribution maps retrieved (2019/09/17) from the IUCN Red List of Threatened Species32. Ensemble SDMs were generated for the seven studied bat species using the R package biomod258, including four models (MaxEnt 3.4, Generalized Boosting Model, Random Forests, Flexible Discriminant Analysis). Models included between 113 and 591 occurrence records (Supplementary Table 5). Species occurrence records were gathered from the online databases GBIF (www.gbif.org) and EUROBATS (https://www.eurobats.org/), from 33 journal publications (Supplementary Table 7), and unpublished records held by co-authors. To reduce spatial bias and spatial auto-correlation, we first removed low-quality records in terms of spatial resolution and taxonomic identification, and then we used the ArcGIS toolbox “SDMtools”59 to thin spatially clustered records. We initially considered 36 environmental variables (16 climatic, six geographic, 13 habitat and three human disturbance) to include in the models. We tested for correlation among variables, and selected among highly correlated ones (|r| > 0.75) the more ecologically relevant or the variable with the stronger effect on model performance on its own for each bat species. Finally, we discarded variables that did not contribute to model performance. The final set of variables used in the models is shown in Supplementary Tables 5–6. Models were run with 10,000 random background points and 1000 maximum iterations. To assess model performance, we used tenfold cross-validations replicates, with 75% of records retained for training and 25% for model testing. We used area under the curve (AUC) of the receiver operator characteristics and True Skill Statistic (TSS) to evaluate the models (Supplementary Table 5). The ten cross-validated replicates were combined to obtain a final predicted environmental suitability map for each of the four modelling methods. Ensemble models were obtained by using AUC values to proportionally weight each method according to its predictive power, excluding models with AUC < 0.75. Spatial metrics were computed using the breadth function implemented in the R package ENMhill60. This function enables computing both absolute and relative spatial breadths from raster data. Potential range size was computed as the number of raster cells above the minimum suitability value estimated for each species. Distribution homogeneity was measured by means of the Hill numbers’ evenness factor61, i.e. through dividing the Hill number of q = 1 by the Hill number of q = 0. Distribution homogeneity takes the unit when the suitability values of all cells is identical and approaches zero as the suitability distribution of cells becomes more uneven.
Statistical analyses and tests
Predator species’ hunting strategy, habitat-use and roosting data were gathered from 45 articles available in the literature (Supplementary Tables 8–10). Breadths of those niche axes were computed by means of Shannon diversity (Hill number of q = 1) using the R package hilldiv. Relationships between computed metrics were analysed through regression analyses using linear models (LM) and linear mixed-effect models (LMM). LMs as implemented in the R package stats were used when analysing species-level data with a single value per species. In contrast, LMMs were used when species-level data contained multiple pseudoreplicated measurements, with dietary niche breadth as fixed effect and species as random effect. Such an approach was used when considering dRER values, as dietary breadth values were characterised using 50 different values to account for uncertainty of the prey phylogenetic trees (details in Supplementary Information). The function lmer as implemented in the R package lme462 was used to compute marginal and conditional R2 values of LMMs. The former describes the proportion of variance explained by the fixed factors, while the latter describes the proportion of variance explained by both the fixed and random factors. p values were fitted and calculated for all effects in the mixed models using the function mixed as implemented in the package afex63. For all statistical tests, significance threshold was set at p = 0.05. All statistical analyses were performed in R64 after averaging the results yielded by both primers unless otherwise stated. Raw files are stored in Zenodo repository65.
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Hutchinson, G. E. Concluding remarks. Cold Spring Harb. Symp . Quant. Biol. 22, 415–427 (1957).
Brown, J. H. On the relationship between abundance and distribution of species. Am. Nat. 124, 255–279 (1984).
Slatyer, R. A., Hirst, M. & Sexton, J. P. Niche breadth predicts geographical range size: a general ecological pattern. Ecol. Lett. 16, 1104–1114 (2013).
Devictor, V. et al. Defining and measuring ecological specialization. J. Appl. Ecol. 47, 15–25 (2010).
Soberón, J. Grinnellian and Eltonian niches and geographic distributions of species. Ecol. Lett. 10, 1115–1123 (2007).
Chase, J. M. & Leibold, M. A. Ecological Niches: Linking Classical and Contemporary Approaches (University of Chicago Press, 2003).
Forister, M. L. et al. The global distribution of diet breadth in insect herbivores. Proc. Natl. Acad. Sci. USA 112, 442–447 (2015).
Shipley, L. A., Forbey, J. S. & Moore, B. D. Revisiting the dietary niche: when is a mammalian herbivore a specialist? Integr. Comp. Biol. 49, 274–290 (2009).
Loxdale, H. D., Lushai, G. & Harvey, J. A. The evolutionary improbability of ‘generalism’ in nature, with special reference to insects: the improbability of generalism in nature. Biol. J. Linn. Soc. Lond. 103, 1–18 (2011).
Peers, M. J. L., Thornton, D. H. & Murray, D. L. Reconsidering the specialist-generalist paradigm in niche breadth dynamics: resource gradient selection by Canada lynx and bobcat. PLoS ONE 7, e51488 (2012).
Abellán, P. & Ribera, I. Using phylogenies to trace the geographical signal of diversification. J. Biogeogr. 44, 2236–2246 (2017).
Brown, J. H., Mehlman, D. W. & Stevens, G. C. Spatial variation in abundance. Ecology 76, 2028–2043 (1995).
Groot, A. T. et al. Genetic differentiation across North America in the generalist moth Heliothis virescens and the specialist H. subflexa. Mol. Ecol. 20, 2676–2692 (2011).
Tucker, C. M. et al. A guide to phylogenetic metrics for conservation, community ecology and macroecology. Biol. Rev. Camb. Philos. Soc. 92, 698–715 (2017).
Alberdi, A. & Gilbert, M. T. P. A guide to the application of Hill numbers to DNA based diversity analyses. Mol. Ecol. Resour. 19, 804–817 (2019).
Vesterinen, E. J. et al. What you need is what you eat? Prey selection by the bat Myotis daubentonii. Mol. Ecol. 25, 1581–1594 (2016).
Aizpurua, O. et al. Agriculture shapes the trophic niche of a bat preying on multiple pest arthropods across Europe: evidence from DNA metabarcoding. Mol. Ecol. 27, 815–825 (2018).
Rebelo, H., Tarroso, P. & Jones, G. Predicted impact of climate change on European bats in relation to their biogeographic patterns. Glob. Chang. Biol. 16, 561–576 (2010).
Chao, A., Chiu, C.-H. & Jost, L. Unifying species diversity, phylogenetic diversity, functional diversity, and related similarity and differentiation measures through Hill Numbers. Annu. Rev. Ecol. Evol. Syst. 45, 297–324 (2014).
Clarke, L. J., Soubrier, J., Weyrich, L. S. & Cooper, A. Environmental metabarcodes for insects: in silico PCR reveals potential for taxonomic bias. Mol. Ecol. Resour. 14, 1160–1170 (2014).
Elbrecht, V. et al. Validation of COI metabarcoding primers for terrestrial arthropods. PeerJ 7, e7745 (2019).
Arrizabalaga-Escudero, A. et al. Assessing niche partitioning of co-occurring sibling bat species by DNA metabarcoding. Mol. Ecol. 27, 1273–1283 (2018).
Galan, M. et al. Metabarcoding for the parallel identification of several hundred predators and their prey: application to bat species diet analysis. Mol. Ecol. Resour. 18, 474–489 (2018).
Vallejo, N. et al. The Diet of the Notch-Eared Bat (Myotis emarginatus) across the Iberian Peninsula analysed by Amplicon Metabarcoding. Hystrix, Ital. J. Mammal. 30, 59–64 (2019).
Aldasoro, M. et al. Gaining ecological insight on dietary allocation among horseshoe bats through molecular primer combination. PLoS ONE 14, e0220081 (2019).
Ma, M. Species richness vs evenness: independent relationship and different responses to edaphic factors. Oikos 111, 192–198 (2005).
Jorge, L. R., Prado, P. I., Almeida-Neto, M. & Lewinsohn, T. M. An integrated framework to improve the concept of resource specialisation. Ecol. Lett. 17, 1341–1350 (2014).
Wilsey, B. & Stirling, G. Species richness and evenness respond in a different manner to propagule density in developing prairie microcosm communities. Plant Ecol. 190, 259–273 (2007).
Russo, D., Jones, G. & Migliozzi, A. Habitat selection by the Mediterranean horseshoe bat, Rhinolophus euryale (Chiroptera: Rhinolophidae) in a rural area of southern Italy and implications for conservation. Biol. Conserv. 107, 71–81 (2002).
Vincent, S., Nemoz, M. & Aulagnier, S. Activity and foraging habitats of Miniopterus schreibersii (Chiroptera: Miniopteridae) in southern France: implications for its conservation. Hystrix https://doi.org/10.4404/hystrix-22.1-4524 (2010).
MacDonald, Z. G., Nielsen, S. E. & Acorn, J. H. Negative relationships between species richness and evenness render common diversity indices inadequate for assessing long-term trends in butterfly diversity. Biodivers. Conserv. 26, 617–629 (2017).
IUCN. The IUCN Red List of Threatened Species. https://www.iucnredlist.org/ (2010).
Bolnick, D. I. et al. The ecology of individuals: incidence and implications of individual specialization. Am. Nat. 161, 1–28 (2003).
Maldonado, K., Bozinovic, F., Newsome, S. D. & Sabat, P. Testing the niche variation hypothesis in a community of passerine birds. Ecology 98, 903–908 (2017).
Van Valen, L. Morphological variation and width of ecological niche. Am. Nat. 99, 377–390 (1965).
Schnitzler, H.-U., Moss, C. F. & Denzinger, A. From spatial orientation to food acquisition in echolocating bats. Trends Ecol. Evol. 18, 386–394 (2003).
Bolnick, D. I. et al. Ecological release from interspecific competition leads to decoupled changes in population and individual niche width. Proc. Biol. Sci. 277, 1789–1797 (2010).
Soberón, J. & Nakamura, M. Niches and distributional areas: concepts, methods, and assumptions. Proc. Natl. Acad. Sci. USA 106(Suppl 2), 19644–19650 (2009).
Belmaker, J. et al. Empirical evidence for the scale dependence of biotic interactions: scaling of biotic interactions. Glob. Ecol. Biogeogr. 24, 750–761 (2015).
de Araujo, C. B., Marcondes-Machado, L. O. & Costa, G. C. The importance of biotic interactions in species distribution models: a test of the Eltonian noise hypothesis using parrots. J. Biogeogr. 41, 513–523 (2014).
Clavel, J., Julliard, R. & Devictor, V. Worldwide decline of specialist species: toward a global functional homogenization? Front. Ecol. Environ. 9, 222–228 (2011).
Gámez-Virués, S. et al. Landscape simplification filters species traits and drives biotic homogenization. Nat. Commun. 6, 8568 (2015).
Zeale, M., Butlin, R. K. & Barker, G. Taxon‐specific P. C. R. for DNA barcoding arthropod prey in bat faeces.Mol. Ecol. 11, 236–244 (2011).
Epp, L. S. et al. New environmental metabarcodes for analysing soil DNA: potential for studying past and present ecosystems. Mol. Ecol. 21, 1821–1833 (2012).
Carøe, C. et al. Single-tube library preparation for degraded DNA. Methods. Ecol. Evol. 9, 410–419 (2018).
Lindgreen, S. AdapterRemoval: easy cleaning of next-generation sequencing reads. BMC Res. Notes 5, 337 (2012).
Zepeda-Mendoza, M. L., Bohmann, K., Carmona Baez, A. & Gilbert, M. T. P. DAMe: a toolkit for the initial processing of datasets with PCR replicates of double-tagged amplicons for DNA metabarcoding analyses. BMC Res. Notes 9, 255 (2016).
Alberdi, A., Aizpurua, O. & Gilbert, M. T. P. Scrutinizing key steps for reliable metabarcoding of environmental samples. Methods Ecol. Evol. 9, 134–147 (2018).
Benson, D. A. et al. GenBank. Nucleic Acids Res. 41, D36–D42 (2013).
Ratnasingham, S. & Hebert, P. D. N. BOLD: The Barcode of Life Data System (www.barcodinglife.org). Mol. Ecol. Notes 7, 355–364 (2007).
Bouckaert, R. et al. BEAST 2: a software platform for Bayesian evolutionary analysis. PLoS Comput. Biol. 10, e1003537 (2014).
Sievers, F. et al. Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol. Syst. Biol. 7, 539 (2011).
Alberdi, A. hilldiv: an R package for integral analysis of diversity based on Hill numbers. bioRxiv https://doi.org/10.1101/545665 (2019).
Jost, L. Entropy and diversity. Oikos 113, 363–375 (2006).
Chao, A., Chiu, C.-H. & Jost, L. Phylogenetic diversity measures based on Hill numbers. Philos. Trans. R. Soc. Lond. B Biol. Sci. 365, 3599–3609 (2010).
Chao, A. et al. An attribute-diversity approach to functional diversity, functional beta diversity, and related (dis)similarity measures. Ecol. Monogr. 41, 40 (2019).
Chiu, C.-H., Jost, L. & Chao, A. Phylogenetic beta diversity, similarity, and differentiation measures based on Hill numbers. Ecol. Monogr. 84, 21–44 (2014).
Thuiller, W., Lafourcade, B., Engler, R. & Araújo, M. B. BIOMOD - a platform for ensemble forecasting of species distributions. Ecography 32, 369–373 (2009).
VanDerWal, J. et al. Package ‘SDMTools’. R package (R Foundation for Statistical Computing, 2014).
Alberdi, A. Analysis of environmental niche modelling projections based on Hill numbers. ENMhill https://github.com/anttonalberdi/ENMhill (2019).
Jost, L. The relation between evenness and diversity. Diversity 2, 207–232 (2010).
Bates, D., Sarkar, D., Bates, M. D. & Matrix, L. The lme4 package. R package version 2, 74 (2007).
Singmann, H., Bolker, B., Westfall, J. & Aust, F. afex: Analysis of factorial experiments. R package version 0.1-145 (R Foundation for Statistical Computing, 2015).
R Development Core Team. R: a language and environment for statistical computing http://www.R-project.org (2008).
Alberdi, A. et al. DNA metabarcoding and spatial modelling link diet diversification with distribution homogeneity in European bats. Zenodo https://doi.org/10.5281/zenodo.3610756 (2020).
A.A. was supported by Lundbeckfonden (R250‐2017‐1351) and the Danish Council for Independent Research (DFF 5051-00033). O.R. was supported by an NERC Independent Research Fellowship (NE/M018660/1), and O.A. was supported by the Carlsberg Foundation’s Postdoctoral Fellowship (CF15-0619). M.T.P.G. acknowledges ERC Consolidator Grant (681396‐Extinction Genomics). JA and IG were supported by the Spanish and Basque Government grants (CGL2012-38610, CGL2015-69069-P, IT754-13, IT1163-19). We are grateful to Fiona Mathews, Daniel Whitby, Roger Ransome, Matt Cook, Carles Flaquer and Martina Spada for providing samples; and Aitor Arrizabalaga, Lide Jimenez, Vilalii Hukov, Olena Holovchenko, Vanessa Mata and Branka Pejić for assistance in the field work. This article is based upon work from COST Action “CLIMBATS—Climate change and bats: from science to conservation”, supported by COST (European Cooperation in Science and Technology).
The authors declare no competing interests.
Peer review information Nature Communications thanks William Symondson and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.
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Alberdi, A., Razgour, O., Aizpurua, O. et al. DNA metabarcoding and spatial modelling link diet diversification with distribution homogeneity in European bats. Nat Commun 11, 1154 (2020). https://doi.org/10.1038/s41467-020-14961-2
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