Human Ebp1 is a member of the proliferation-associated 2G4 (PA2G4) family and plays an important role in cancer regulation. Ebp1 shares the methionine aminopeptidase (MetAP) fold and binds to mature 80S ribosomes for translational control. Here, we present a cryo-EM single particle analysis reconstruction of Ebp1 bound to non-translating human 80S ribosomes at a resolution range from 3.3 to ~8 Å. Ebp1 blocks the tunnel exit with major interactions to the general uL23/uL29 docking site for nascent chain-associated factors complemented by eukaryote-specific eL19 and rRNA helix H59. H59 is defined as dynamic adaptor undergoing significant remodeling upon Ebp1 binding. Ebp1 recruits rRNA expansion segment ES27L to the tunnel exit via specific interactions with rRNA consensus sequences. The Ebp1-ribosome complex serves as a template for MetAP binding and provides insights into the structural principles for spatial coordination of co-translational events and molecular triage at the ribosomal tunnel exit.
The ErbB3 receptor-binding protein (Ebp1, 394 residues) is a highly conserved, widely expressed, and multifunctional eukaryotic protein. It has attracted considerable attention due to its regulative role in cancer progression, although the question of being “friend or foe”1 is still unanswered. This uncertainty is based on the contradictory functions of the two splice variants p42 and p48, with p42 (lacking 54 N-terminal residues) acting as tumor suppressor and p48 promoting cell proliferation1. First described as part of the ErbB3 receptor pathway2, it has been further on identified as an IRES-trans-acting factor (ITAF45) initiating viral mRNA translation3. Moreover, Ebp1 binds to ribosomes and was also shown to inhibit phosphorylation of initiation factor eIF2α4. Ebp1 itself is phosphorylated on multiple Ser/Thr sites, which promotes interactions with ErbB3 and Akt kinase involved in apoptosis (Ser360 phosphorylation) (reviewed in ref. 1). Further kinases described to interact with Ebp1 are protein kinase C (PKCδ), dsRNA-activated kinase (PKR), p21-activated serine–threonine kinase (PAK1), and cyclin-dependent kinase (CDK2).
X-ray structures of Ebp15,6 confirmed the predicted methionine aminopeptidase (MetAP) fold, also described as pita-bread fold, with a deep pocket on its concave surface accommodating the active site in MetAPs7. MetAPs are metalloproteases essential for all kingdoms of life that cleave-off the first methionine from the growing polypeptide chain as soon as a length of 40 amino acids is reached8. Together with the ribosome-associated chaperones RAC and Ssb, they belong to the first interaction partners of nascent chains (NCs) emerging from the ribosome9,10. MetAPs are classified into two types7, with MetAP-2 including a helical subdomain (insert domain) of ~60 residues (Supplementary Fig. 1). Ebp1 is MetAP-2 like and mainly distinguished from the MetAPs by the missing catalytic activity, a shorter N-terminus (~150 residues missing) and a C-terminal extension of about 50 residues harboring a highly positive charged patch including six consecutive lysine residues (Supplementary Fig. 1). The lysine cluster has been shown to act as major nucleolar localization signal and to be involved in phosphoinositide binding11. Positive surface charges of Ebp1, indicative for the confirmed dsRNA-binding properties of Ebp15, cluster on the convex side and include a charged surface loop (residues 62–72) and the C-terminal region, although the lysine cluster was disordered and not part of the X-ray models (missing 30 C-terminal residues)5,6.
Ebp1 has been further on assigned as the human homolog of the 60S pre-ribosomal nuclear export factor Arx1 from Saccharomyces cerevisiae12, but any direct evidence for such functional homology is still missing. In particular, Ebp1 lacks structural features that are centrally involved in ribosome biogenesis factor interaction in yeast Arx113,14, and it does not bind to nucleoporins12, as would be required for a pre-60S nuclear export factor. Furthermore, Ebp1 stably binds to mature 80S ribosomes in vivo as shown in this study, while Arx1 is known to exclusively bind to 60S ribosomal subunits during maturation. Intermediate resolution cryo-electron microscopy (cryo-EM) structures of Arx1 bound to the yeast 60S (pre-)ribosome were reported previously13,15, and finally refined to 3.4 Å resolution14. These cryo-EM reconstructions confirmed the structural homology to the MetAPs and visualized Arx1 in the same binding site on the ribosomal tunnel exit that was observed for a bacterial MetAP–ribosome complex in a low-resolution structure of >10 Å16.
Recently, MetAPs enzymatic activity was shown in yeast to depend on its interaction with rRNA expansion segment ES27L, one of the longest tentacle-like dsRNA insertions (714 nucleotides in human 28S rRNA) typical for eukaryotic ribosomes17. Unexpectedly, this interaction was at the same time found to control the accuracy of ribosomal mRNA decoding. The mechanism is elusive, and sequence conservation in ESs is generally low and varies within species and even tissues18. Deletion of ES27L is lethal in the ciliate Tetrahymena thermophila19, and its close proximity to the tunnel exit perfectly positions it for the coordination of co-translational interaction partners. The exact role of ESs in translational control, the precision of protein biosynthesis, and maturation and folding is not understood.
To shed light on the role of Ebp1 in translational regulation and molecular mechanisms underlying its multiple cellular functions, we wanted to address its structure in the context of the translation machinery using cryo-EM single-particle analysis. Here, we present the cryo-EM reconstruction of Ebp1 bound to the human 80S ribosome at a resolution ranging from 3.3 to ~8 Å. Ebp1 binds on top of the tunnel exit by several distinct interaction sites, with a remodeled rRNA helix H59 and ES27L presenting the most prominent contacts. Our data provide a rationale of the structure, dynamics, and function of ESs at the ribosomal tunnel exit. The structure provides a generalized view of MetAP-fold recognition by the ribosome, and reveals structural principles for spatial coordination of co-translational events at the ribosomal tunnel exit.
Cryo-EM structure of Ebp1 bound to the human 80S ribosome
Binding of factors to the ribosomal surface is often governed by considerable flexibility and conformational heterogeneity that can only be addressed by a hybrid structural biology approach integrating state-of-the-art cryo-EM analysis and high-resolution X-ray structures. The 1.6 Å X-ray structure of Ebp1 was solved in our lab previously5. To gain insights into Ebp1 binding to the human 80S ribosome, we used cryo-EM single-particle analysis, and determined the structure of full-length Ebp1 (p48 isoform) in complex with puromycin-treated 80S ribosomes purified from HeLa cells (Supplementary Fig. 2, Supplementary Table 1). Interestingly, ~25% of the purified ribosomes were already decorated with endogenous Ebp1 (dataset 1), and this fraction could be raised up to ~75% upon addition of recombinant Ebp1 (dataset 2). The in vivo pulled-out and in vitro-reconstituted Ebp1–ribosome complexes were virtually identical, indicating that no significant conformational changes or rearrangements were introduced by reconstitution of the complex with the purified ribosomes. Therefore, the two datasets were merged to obtain a single higher resolution structure (Fig. 1a). In brief, ribosomal particles were automatically located and subjected to several consecutive rounds of in silico sorting. Initial refinement of the 34,467 particles retained after sorting resulted in a reconstruction at 3.3 Å global resolution (Supplementary Fig. 3a). Lower local resolution for the 40S ribosomal subunit (Supplementary Fig. 3d) indicated conformational heterogeneity originating from ribosomal intersubunit rotation, which we compensated for by separating the Ebp1–ribosome complex into two independently refined segments (“2-body” approach), comprising the 40S ribosomal subunit and the 60S ribosomal subunit plus Ebp1 and rRNA ES27L, respectively. This approach resulted in improved global and local resolution (Supplementary Fig. 3b, e) and density quality for the two ribosomal subunits and directly ribosome-associated Ebp1 segments. This set of cryo-EM densities thus allowed us to analyze the interface between Ebp1 and the ribosomal tunnel exit at a resolution of better than 4 Å (Supplementary Fig. 3e, g), with amino acid side chains and rRNA bases clearly resolved for the entire interface. In contrast, lower local resolution for more peripheral regions of Ebp1 and ES27L hindered interpretation of the Ebp1–ES27L interface, and suggested additional conformational mobility independent from the ribosomal 60S subunit for these two components (Supplementary Fig. 3b). We thus subjected particles to another round of refinement, in which we treated the Ebp1–ES27L segment as a separately refined body (“3-body” approach). Local resolution and interpretable features for ES27L and the very peripheral Ebp1 segments interacting with ES27L improved using this approach (Supplementary Fig. 3c, f, h), and the resulting cryo-EM density thus allowed detailed analysis of the Ebp1–ES27L interface. Supplementary Fig. 4 shows the full set of cryo-EM densities obtained from the three individual refinement approaches and highlights the density segments that were used to address the different aspects of the Ebp1–ribosome interaction.
Ebp1 occupies the ribosomal tunnel exit and recruits ES27L
The MetAP fold of Ebp1 as determined by X-ray structure analysis binds to the 80S ribosome as a rigid body (root-mean-square deviation (rmsd) for 352 Cα-atoms of 1.0 Å) and thus the model could be directly fitted into the cryo-EM density (real-space correlation coefficient (cc) of 64%). In particular, the relative orientation of the insert domain in respect to the protein core remains unchanged. Ebp1 binds with its concave surface, harboring the active site in MetAPs, directly on top of the ribosomal tunnel exit and is sandwiched between the exit and ES27L (Fig. 1a). Thus, access to the tunnel exit is completely blocked for any globular NC-binding factor, and only a small lateral gap between Ebp1 and the tunnel exit is left (Supplementary Fig. 5). Ebp1 establishes contacts with ribosomal components all around the inner ring of the tunnel exit, including proteins eL19, uL23, uL24, and uL29 as well as the 5.8S rRNA (helix 24) and the 28S rRNA (helices H24, H47, H53, and H59) (Fig. 1b, c). The conformations of all these ribosomal elements are almost identical as previously described in cryo-EM structures of the vacant human 80S ribosome20,21,22. The single and pronounced exception is rRNA helix H59, which undergoes significant remodeling upon Ebp1 binding, identifying it as a conformationally dynamic adaptor on the 60S ribosomal subunit.
Owing to their dynamic behavior, long ribosomal RNA expansion segments are typically not resolved in high-resolution cryo-EM reconstructions, with few exceptions in which their conformational landscape is confined by specific interactions with either the ribosomal core or ribosome-associated factors. For one of the longest eukaryotic rRNA ESs, ES27L, two distinct conformations were described23,24: ES27L either aligns with the ribosomal 40S/60S subunit interface (“L1-position”; “ES27L-L1”) or projects toward the ribosomal tunnel exit (“exit-position”; “ES27L-exit”) (Supplementary Fig. 6). The two conformations were suggested to play a role in coordinating access of non-ribosomal factors to the ribosomal tunnel exit23. In the human Ebp1–ribosome complex, ES27L is resolved in the exit position and the ES27L-B-arm (370 nucleotides, 2912–3281) reaches over the 60S tunnel exit (Fig. 1a, c) as previously only observed for 80S complexes from yeast13,14,25. The conformational changes within ES27L-B are immense, with an almost 120° rotation around its anchor point on the ribosomal surface (at ES27L-A of 28S rRNA) and maximal distances of the outermost visible parts (~1/3 of ES27L-B) moving more than 20 nm (Supplementary Fig. 6). The ES27L-B exit position is stabilized by an extensive contact of 1000 Å2 with Ebp1 (overall Ebp1–ribosome interaction surface: 2500 Å2) and an additional smaller contact with eL38. Even in the exit position, ES27L-B shows considerable plasticity in the eL38-binding region and is resolved throughout only after in silico particle sorting focused on the ES27L conformation (Supplementary Fig. 2d). Using this approach, several distinct ES27L-B conformations were identified with Ebp1 acting as a pivot accommodating various ES27L-B orientations (Supplementary Movie 1).
The Ebp1–ES27L interaction
MetAP binding to ES27L has been recently identified in yeast as an important principle increasing enzymatic activity and controlling translation fidelity17, but the underlying principles are unclear. ES27L sequences are generally not conserved in eukaryotes: they vary from an AU-rich base composition in Drosophila (32% GC) via an even content in yeast (57% GC) up to an extreme GC-rich version in Homo sapiens (89%)20 and ES27L length has been more than quadrupled from fungi (159 nts in baker’s yeast) to metazoans (714 nts in humans) for so far unknown reasons. Combining already available structural information from the Arx1–ES27L interaction in yeast with our cryo-EM reconstruction of the human Ebp1–ribosome complex, we could build a model for the corresponding regions of human ES27L, including 100 nts of ES27L-B reaching over the tunnel exit and parts of ES27L-C (30 nts). Although the base pairs are not resolved due to the extensive conformational plasticity of the central ES27L-B region (Supplementary Movie 1), the regular spacing of the A-form RNA helix emanating from the well-defined ES27L-A stem and resolved base pair mismatches allow for unambiguous extension of ES27L-B from the ribosomal core to Ebp1. The ES27L-B model allows for the definition of three specific Ebp1–ES27L contacts (Fig. 2a). Two of them involve N-terminal helices that are part of the conserved MetAP fold, while the last one is mediated by the Ebp1-specific C-terminal extension. On the RNA side, two consensus sequences are involved that are conserved from yeast to metazoans (Fig. 2b).
Consensus sequence 1 (cs1) contains two base mismatches, namely a purine–purine and a GU-wobble base pair, and is recognized by N-terminal regions of Ebp1 (Fig. 2a, b). The sterically demanding purine–purine interaction (G2947-A3247) pushes the phosphoribose backbone around A3247 into a positively charged loop (K62IFKKEKEMKK, interacting residues are in italics) on the convex side of Ebp1 (Fig. 2c). The “exposed” phosphate group of A3247 is accommodated in a “P-loop” (phosphate-binding loop)-like structure typical for the binding of the β-phosphate moieties of NTPs (N: any nucleotide) within the large superfamily of P-loop-containing NTPases (Pfam clan CL0023). In Ebp1, the phosphate approaches four main chain nitrogens oriented toward the phosphate group. The neighboring 5′-ribose of the conserved guanosine (G3246) is recognized by hydrophobic ring stacking on the two exposed hydrophobic Ebp1 residues Ile63 and Phe64, while the ribose of A3247 and the phosphate group of the 3′-nucleotide (C3248) are clamped in between the two conserved Ebp1 residues Lys65 and Lys66.
The GU-wobble base pair (G2950-U3244) within cs1 is necessary in order to expose G2950 into the minor groove of the A-RNA helix where it is recognized by Thr19 on the N-terminal Ebp1 helix α1 (Fig. 2d). Interactions around the GU wobble are completed by Ebp1 residues exposed by neighboring turns of helix α1 (Asp15, Lys22). Both mismatch recognitions within cs1 are conserved in yeast for the Arx1–ES27L interaction, as observed upon in-depth analysis of the original cryo-EM density14 by building the respective model for yeast ES27L (Supplementary Fig. 7a, b). However, ES27L in yeast has a slightly different orientation relative to Arx1 (rotational tilt), which results in an interaction of the neighboring GU wobble of cs1 (U1997-G2024) with Arx1 (Fig. 2b; Supplementary Fig. 7b).
The adjacent consensus sequence 2 (cs2) is characterized by the accumulation of mismatches rather than sequence homology (Fig. 2b), which reflects the different readout principles mediated by the C-terminal Ebp1- and Arx1-specific regions. Human cs2 is dominated by purine–purine mismatches, which overall result in a extensive widening of the major groove (by more than 75%) (Fig. 2a, e; Supplementary Fig. 7c) that typically is narrow and deep in A-RNA and not accessible for protein interactions. While the AG/GA tandem mismatch stretches the width of the RNA helix, it is constrained at the site of the GG mismatch (G2957/G3237) and best fitted by a cross-strand purine stack. The X-ray models of Ebp15,6 lack the most C-terminal 33 amino acid residues directly following the important phosphorylation site Ser360 at the end of helix α10. In our cryo-EM reconstruction, the C-terminus of Ebp1 is resolved (Fig. 2a) and projects toward the widened major groove, where it forms an α helix (αC) with the highly basic lysine cluster (S363RKTQKKKKKKASKTAENA381, helical region in italics, side chains not traceable) that entirely fills the groove (Fig. 2e). This kind of major groove readout by positively charged α helices is well known from the so-called ARMs (arginine-rich motifs) as found i.e., in viral transactivation (HIV-1 Rev-peptide/Rev-response element RRE)26 and later on in the signal recognition particle (SRP68/SRP RNA)27. Accordingly, we define this motif as “KRM”, with the arginine (R) being replaced for lysine (K). In Arx114, only one single turn of helix αC is formed, and the chain does not penetrate the major groove as the RNA continues in a regular A-form and yeast ES27L-B is terminated. The interaction of this single αC turn including two positive charges with the most distal ES27L-B GU wobble next to the tetranucleotide loop is reminiscent of the Ebp1–ES27L contact, but the overall architecture of the interaction site is different. All MetAPs are missing a corresponding positively charged C-terminal region (~50 residues less) and thus, this ES27L contact at cs2 is specific for the PA2G4 family like Ebp1 and Arx1.
The general docking site for NC-associated factors revisited
The second half of the Ebp1–ribosome interface is formed by the inner ring of the ribosomal tunnel exit. The tunnel exit is a hot spot in cellular activity and a plethora of NC-associated factors compete for similar binding sites (reviewed in refs. 9,28). Most importantly, uL23 has been described as a general docking site for NC-interacting chaperones (RAC, NAC, ERj1p, Trigger factor), protein targeting factors plus their associated membrane insertion machineries (SecA and SRP/translocon, Get pathway, Oxa1), and NC-modifying enzymes like the MetAPs.
In the Ebp1–ribosome complex, docking of the insert domain of Ebp1 to uL23 constitutes the largest interface to the inner tunnel exit ring (500 Å2) (Fig. 3a). The interface includes four α helices (α6 and α8 of Ebp1) and has a methionine-rich hydrophobic core that is surrounded by hydrophilic and charged contacts. Most strikingly, the very C-terminus of uL23 forms a salt bridge with Arg243 of Ebp1 at the start of the insert domain (Fig. 3a). The insert domain also contacts ribosomal elements around uL23, and thus this general docking site is rather extended (1200 Å2). Overall, the extended docking site confers to 80% of the Ebp1 interaction with the inner exit ring (1500 Å2). All minor interactions that complete the overall triangular docking of Ebp1 to the exit, form small “lysine-trident” contacts with either the phosphoribose backbone of 5.8S rRNA helix 24 (Fig. 3a), or with the connection of uL24/28S rRNA helix 24 (not shown).
This extended general docking site is unique among the so far described tunnel exit interactions. Still, the mode of central uL23 recognition is very similar as found for the bacterial Trigger factor29. The N-terminal domain of Trigger factor, a co-translational chaperone that crowches over the tunnel exit30, also forms hydrophobic interactions and binds the very C-terminus of bacterial uL23 (Fig. 3b). However, this contact constitutes the major interaction in the Trigger factor–ribosome complex, and binding of the chaperone to the exit is known to be transient31.
Also typical for many exit tunnel interactions, docking occurs in tandem with the uL23-neighboring protein uL29. In the Ebp1–ribosome complex, the contact with uL29 is small and appears rather flexible as it involves van der Waals interactions of small residues (glycines and alanines) (Fig. 3a). In comparison, SRP establishes a major interaction with both uL23 and uL29 when binding to the ribosome for targeting of nascent secretory and membrane proteins to the ER membrane32,33,34 (Fig. 3c). Interestingly, the insert domain of Ebp1 does not sterically exclude binding of the SRP GTPase SRP54, which is centered more toward uL29. However, the flexibly linked signal sequence-binding domain of SRP54 (SRP54M) cannot be positioned over the tunnel exit in the presence of Ebp1 (or MetAP-2). Similarly, the protein conducting channel of the ER membrane (Sec61αβγ) strongly binds to the same site35,36. While Sec61γ binds to uL23, Sec61α binds to uL29 and H50 of 28S rRNA at the base of the tunnel exit (Fig. 3d). Finally, cryo-EM reconstructions of the co-translational eukaryotic chaperones NAC (nascent polypeptide-associated complex)37 and the ER membrane protein ERj1p (Hsp40-type co-chaperone of ER-lumenal Hsp70 BiP)38 also revealed binding to the general docking site, however, due to limited resolution atomic models thereof could not be built and further data are necessary for detailed descriptions. The general physiological necessity for a triage between protein folding and co-translational targeting, depending on NC-associated factors binding to the general docking site, has been remarked and described earlier9,28.
H59 forms a dynamic adaptor to the insert domain of Ebp1
A striking feature of the Ebp1–ribosome interaction is the extension of the Ebp1-binding site beyond the general docking site toward eL19 and helix H59 of 28S rRNA (Fig. 4). This contact is even larger (700 Å2) than the uL23/uL29 interface, and unique among all other ribosomal complexes with available structural information (except of its yeast homolog Arx114). Both eL19 and H59 (also termed ES24L; 21 nts in human 28S rRNA, 2698–2718) are eukaryote specific. In general, the globular N-terminal domain of eL19, which is essential for cell viability and ribosome biogenesis39, binds to H59 at its closing loop (G2705GUUCCG2711 in human 28S rRNA). In all mammalian ribosome structures solved so far, guanine G2711 is bulged out and anchors H59 on the ribosomal surface by interactions with H53 and the H58-59 connection (defined here as “locked position”) (Supplementary Fig. 8a). In the Ebp1–ribosome complex, the loop is completely remodeled and bridges over toward the highly basic helix α6 of the Ebp1 insert domain (Fig. 4a; Supplementary Fig. 8a, b). This remodeling coincides with a lateral displacement of H59 by up to 10 Å (at G2711), which releases the guanine anchor G2711 and rotates H59 further onto eL19 (“docked position”). Thus, H59 serves as a conformationally dynamic adaptor for Ebp1 recruitment to the tunnel exit (Supplementary Movie 2). It is unlikely that the observed remodeling of H59 also occurs in yeast, because the bulged-out nucleotide is not present and in all yeast ribosome structures H59 is found in the “Ebp1-like” docked position.
The cryo-EM density around the interaction site approximates the overall resolution of 3.3 Å, which allows for the precise fitting of the interacting partners and especially the region around Ebp1 helix α6 (Fig. 4b) as a main docking partner within the insert domain (Supplementary Fig. 1). Overall, the interaction is dominated by π–π and π–cation stackings and two Watson–Crick-like base readouts (U2708 and G2711) with the protein main chain (Fig. 4c). Uridine U2708, which points into the H59 RNA loop in structures of vacant ribosomes (Supplementary Movie 2), is bulged out and accommodated in a binding pocket created by residues of Ebp1 helix α6 and its N-terminal loop (Fig. 4d; Supplementary Fig. 8b). While the uridine base is sandwiched between Asn254 and Phe266, its Watson–Crick edge is perfectly read out by the protein main chain (Tyr255 and Leu257). The interaction is completed by Ser267 hydrogen bonding to the ribose moiety. An almost identical interaction has been observed as a major determinant in the Arx1–pre-60S interaction14, although the base-specific readout was not described (Supplementary Fig. 8c). As this specific readout is mediated by the main chain, the conservation of the binding pocket is not obvious in respect to the MetAP-2 family; however, the overall features of this docking region are preserved (Supplementary Fig. 1). The two subsequent nucleotides of H59 (C2709 and C2710) are stacked on top of the loop and held in place by the capping π–cation stacking with Arg271 (Fig. 4c).
Upon H59 remodeling, nucleotide G2711 undergoes the largest movements, and like U2708, it is fixed in a base-specific binding pocket at the N-terminal end of Ebp1 helix α6 in the docked position (Fig. 4e). Unlike the binding site for U2708, G2711 is accommodated in a composite pocket formed by Ebp1 and helix α3 of eL19. Here, the base is stacked between Arg263 of Ebp1 and Gln40 of eL19, and the Watson–Crick readout is mediated by the Ebp1 main chain (Leu257 and Lys258). The guanine base is further hydrogen bonded to side chains within helix α3 of eL19 (Ser37 and Gln39), which itself also contacts Ebp1 directly (Asp34 and Asp36). The respective nucleotide is missing in yeast, although an Arx1-eL19-binding pocket would be present in principle. Finally, as a consequence of the H59 re-arrangement, the rRNA helix is rolled onto helix α3 of eL19, which by itself establishes a ribosome internal specific base recognition of guanine G2707 by Glu47 complemented by charged interactions (Lys43 and Lys46) (Fig. 4f).
Taken together, the interaction of the Ebp1 insert domain with H59 of 28S rRNA defines this eukaryotic rRNA expansion segment as a dynamic adaptor for PA2G4/MetAP-2-fold proteins at the ribosomal tunnel exit. The ribosomal interaction site (H59 and eL19) as well as the respective Ebp1/MetAP-2 regions are structurally conserved in mammals (rmsd of 1.7 Å for 287 Cα-atoms; Supplementary Fig. 1), indicating that binding of MetAP-2 and possibly other factors might employ the conformational plasticity of H59 in a similar manner.
The structure of the Ebp1–ribosome complex revealed the importance of plastic rRNA expansion segments in specific ligand recruitment (or vice versa) at the ribosomal tunnel exit. Besides helix H59, the tentacle-like ES27L was identified in its ES27L-exit conformation to serve as a major rRNA docking site for Ebp1. In a most recent cryo-EM reconstruction of the yeast ribosome–NatA complex, the trimeric NatA complex (Naa10/Naa15/Naa50) was also shown to recruit ES27L in the exit position by binding to the closing tetraloop25 adjacent to the cs2 region. NatA was described with a unique mode of ribosome interaction, contacting ESs in three out of four binding patches. Interestingly, the NatA-binding site is directly adjacent (shifted toward ES7a and ES39a) to the Ebp1/Arx1-binding site, with only the PA2G4-specific C-terminal extension of the MetAP-fold clashing (Fig. 5a). Thus, while according to structural comparison, Ebp1 and NatA binding are likely mutually exclusive, concomitant MetAP/NatA binding would be possible25 (Fig. 5b). Moreover, the suggested minimal NC length of 50 amino acids in order to reach the Naa10 catalytic site25 is only ten residues longer as found for the MetAPs, strongly indicating a direct handover mechanism. The distal end of ES27L-B undergoes a conformational transition from the Arx1- (MetAP-) to the NatA-bound state with a 30° bending (translation of 30 Å of the tetraloop) (Fig. 5a). Therefore, the MetAP/ES27L-B contact is likely to be disrupted upon NatA binding, which potentially prepares subsequent dissociation of MetAP.
The structural homology of the PA2G4 member Ebp1 with the MetAP family and the resulting competition for ribosome binding raises questions regarding physiological readout (schematized in Fig. 6) and potential pathological implications. Evidently, binding of Ebp1 and MetAPs or Nats is mutually exclusive, and persistent Ebp1 binding to the ribosomal tunnel exit would consequently prevent any co-translational protein modifications by either the MetAPs or the NATs with severe effects on the cellular proteome. Whether also protein synthesis per se is arrested upon Ebp1 binding is more challenging to address on a structural basis. The plurality of ribosomal protein and rRNA-binding events within the Ebp1–ribosome complex, in particular the arrest of conformational dynamics of ES27L, might induce conformational changes in the ribosome that permit long-scale signal transfer toward the peptidyl transferase center (PTC) and thus impact on translational kinetics. However, our cryo-EM reconstruction does not indicate such a conformational signal transfer upon Ebp1 binding, as the conformation of ribosomal proteins and rRNA in the PTC remains unaltered compared with the vacant mammalian 80S ribosome. As observed upon focused 3D classification, the same holds true for conformational dynamics of the ribosomal small subunit, which recapitulates the canonical ratcheting and rolling motions of the translational elongation cycle also after Ebp1 binding, as well as for translational elongation factor binding, which seems not to be affected (Supplementary Fig. 9). Therefore, our cryo-EM data do not per se support a consequent mechanistic translation inhibition upon Ebp1 binding. However, during elongation, any NC would be sterically hindered in exiting the ribosome by the blockade via (or potential binding to) Ebp1 and the conformational fixation of ES27L in the exit position, which collectively might affect translation in an indirect way. Whether such stalled Ebp1–ribosome–NC complexes exist and if the Ebp1–ribosome interaction may alter in the presence of a NC remains to be addressed.
Ebp1 inhibits phosphorylation of initiation factor eIF2α4, and phosphorylation is likely to be regulated by the phosphorylation status of Ebp1 itself. As estimated from computational particle sorting during cryo-EM data processing (Supplementary Fig. 2), ~25% of our ribosomes purified from HeLa cells were already decorated with Ebp1, a fact which in previous studies has been either overlooked or not been commented on. This occupancy could be raised up to ~75% upon addition of recombinant Ebp1. In our studies, we cannot resolve any putative phosphorylation at the Ebp1 C-terminus, and especially that of Ser360. However, Ser360 is in direct vicinity to the phosphoribose backbone of the ESL27L-B cs2 region (Fig. 2a, e), and a detrimental effect of its phosphorylation for ES27L binding can be assumed. This indicates that Ebp1 binding to mature ribosomes requires non-phosphorylated Ser360 and further C-terminal residues, as the subsequent Ebp1 helix αC penetrates deeply into the major groove of ES27L-B and engages in electrostatic interactions with the negatively charged phosphate backbone of the rRNA.
HeLa cells are derived from cervical cancer, and Ebp1 is well known as an essential regulator of cell growth, with the direction of regulation and effects on cancer progression still being debated (reviewed in ref. 1). This raises the question of whether Ebp1 binding to ribosomes is enhanced in HeLa cells compared with healthy cells or cells from other tissues. To this end, we compared the overall Ebp1 occupancy on ribosomes in HeLa cells to HEK293 cells derived from embryonic kidney cells, which at least by qualitative immunoblot analysis did not reveal any significant differences (Supplementary Fig. 10). Further, overall data analysis of gene expression profiling in different cancer cell lines indicates on average not more than about twofold elevation of Ebp1 expression over paired normal tissues (http://gepia.cancer-pku.cn/). The effect of Ebp1 on cell proliferation in cancer depends on its isoform: while the p48 isoform has pro-oncogenic effects, the p42 isoform is a tumor suppressor. Isoform p42 can directly bind to the ErbB3 receptor40, but lacks the 54 N-terminal residues of p48 that are involved in recognition of the cs1 element of ES27L-B and are an integral feature of the MetAP-like fold. The p42 isoform could therefore have different properties in terms of overall conformation, folding and in particular ribosome binding. While experimental validation is missing, the truncated N-terminus of p42 indicates that binding to the ribosome might be impaired. Thus, Ebp1 binding to ribosomes is likely isoform-specific, and consequently might correlate with tumor progression. Dysregulation of translation is a frequent feature of fast proliferating cells, e.g., in neoplasia41, and thus an inhibitory effect of p48 on translation could have protective effects under such conditions. The correlation of the multiple (patho-)physiological and cellular functions with respective molecular mechanisms will be the next challenge in Ebp1-related research.
The total human mRNA was extracted from HEK293-S3 cells using the Direct-zol RNA Miniprep kit (Zymo Research) and reversed transcribed into cDNA utilizing the Maxima first strand cDNA synthesis kit with dsDNase (Thermo Scientific). The coding sequence of the EBP1 gene was amplified by PCR using the HEK cDNA as a template and the primers Ebp1NcoI_Fwd (CATGCCATGGCTTCGGGCGAGGACGAGCAAC) and Ebp1BamHI-Rev (CGGGATCCTCAGTCCCCAGCTTCATTTTCTTC). The ~1200 bp PCR product was digested with NcoI/BamHI restriction nucleases (NEB) and ligated into the pET24d-His6-linker-TEV (tobacco edge virus) resulting in the pET24d-His6-linker-TEV-Ebp1 plasmid.
Ebp1 was expressed from the pET24d-His6-linker-TEV-Ebp1 plasmid in E. coli Rosetta 2 cells (Novagen) using autoinduction medium42. Cells were cultured at 37 °C until reaching OD600 0.6–0.8, upon which the temperature was shifted to 21 °C and expression was continued for 16 h. Cells were harvested and resuspended in lysis buffer (40 mM HEPES KOH pH 7.5, 1 M NaCl, 10 mM MgCl2, 10 mM KCl, 40 mM Imidazole, 0.02% 1-thioglycerol) supplemented with protease inhibitor cocktail (Roche) at 1× final concentration. Resuspended cells were lysed utilizing a microfluidizer (Microfluidics Corp.), and the lysate was cleared via centrifugation for 20 min at 20.000 × g and 4 °C. The cleared lysate was applied to Ni-IMAC (immobilized metal affinity chromatography) (GE Healthcare) and thoroughly washed with lysis buffer. Ebp1 was eluted in 20 CV using elution buffer (40 mM HEPES KOH pH 7.5, 1 M NaCl, 10 mM MgCl2, 10 mM KCl, 500 mM Imidazole, 0.02% 1-thioglycerol). The eluate was transferred to a 8.000 MWCO dialysis tubing (Spectrum Labs) including TEV protease at a final concentration of 40 µg/mL and dialyzed against dialysis buffer (20 mM HEPES KOH pH 7.5, 500 mM NaCl, 5 mM MgCl2, 5 mM KCl, 5 mM 2-mercaptoethanol) at 4 °C. TEV digested Ebp1 was isolated via reverse Ni-IMAC and in a final step purified via Superdex 75 (GE Healthcare) size-exclusion chromatography (SEC) equilibrated in SEC Buffer (20 mM HEPES KOH pH 7.5, 5 mM Mg(OAc)2, 175 mM KOAc, 1 mM tris(2-carboxyethyl)phosphin). Ebp1 containing fractions were pooled, snap frozen in liquid nitrogen, and stored at −80 °C until further use.
Human non-translating 80S ribosomes were isolated from HeLa cells in a protocol as described previously43 that we adapted from a large-scale setup44. Briefly, HeLa cells were grown in suspension cultures and harvested cells (1 × 108 cells per 100 mL) were lysed with detergent. After clearing of the lysate from debris and membranes, ribosomes were purified via centrifugation through a sucrose cushion. The pellet was resuspended, treated with puromycin and the monosomes further purified in a sucrose gradient. The monosome peak was collected and concentrated to 1 mg/mL in the same physiological buffer as Ebp1 (SEC buffer), aliquoted and snap frozen in liquid nitrogen and stored at −80 °C. The typical yield is 1 mg per 1 × 108 cells.
HEK293 and HeLa cell lysates were separated on a 12.5% SDS-PAGE gel and transferred onto a Protran nitrocellulose membrane (Amersham). The membrane was stained with Ponceau-red S to detect the total protein and probed with antibodies against Ebp1 (N-terminus, ABE43, 1:1000, Merck Millipore). Ebp1 was detected with peroxidase-conjugated goat anti-rabbit IgG antibody (111-035-144, 1:5000, Jackson ImmunoResearch) and visualized with the ECL Western Lightning Ultra (Perkin Elmer), according to the manufacturer’s protocol.
Two batches of EM grids were prepared. The first batch used for collection of dataset 1 contained the in vivo pulled-out Ebp1–ribosome complex without addition of recombinant Ebp1. The second batch used for collection of dataset 2 contained the same purified ribosomes, but supplemented with recombinant Ebp1 (p48 isoform) to increase Ebp1 occupancy on the ribosomes. Right before freezing, Quantifoil Multi A holey carbon supported grids (Quantifoil, Multi A, 400 mesh) were glow-discharged for 10 s in oxygen atmosphere using a Solarus plasma cleaner (Gatan, Inc.). In total, 3 μL of freshly prepared samples (100 nM ribosomes without/with a eightfold excess of recombinant Ebp1) were directly applied to glow-discharged grids. Under a blot force of 0 at 100% humidity, the grids were blotted for 3 s with Whatman #1 filter papers using a Vitrobot Mark IV (FEI Company) operated at room temperature, and then immediately plunge-frozen in liquid ethane cooled with liquid nitrogen.
Cryo-EM data were acquired on a Titan Krios transmission electron microscope (Thermo Fisher/FEI Company) operated at an acceleration voltage of 300 keV. Two data sets were collected. The first dataset (dataset 1) was acquired on a K2 Summit direct electron detector (Gatan, Inc.) at an object pixel size of 1.07 Å in Latitude (Gatan Company) with a target defocus range of −1.5 to −3.0 µm. Micrographs were acquired using dose fractionation to record 40 frames per exposure with a dose rate of 0.9 electrons per Å2 per frame, resulting in a total dose of 39 electrons per Å2 per micrograph. The second dataset (dataset 2) was acquired on a K3 direct electron detector (Gatan, Inc.) at an object pixel size of 1.07 Å. Data were collected using the SerialEM software package with a target defocus range of −0.5 to −2.0 µm. Micrographs were acquired using dose fractionation to record 20 frames per exposure with a dose rate of 1.85 electrons per Å2 per frame, resulting in a total dose of 37 electrons per Å2 per micrograph.
All steps of image processing are summarized in a visual flow chart (Supplementary Fig. 2). Both data sets were initially processed separately, but following the same workflow. All processing steps were performed with the RELION 3.0-beta software package, unless stated otherwise45. Movie stacks were motion-corrected using MotionCor2 with the number of patches set to 5 × 546. Estimation of contrast transfer function (CTF) parameters was performed with Gctf on the motion-corrected micrographs47. To generate reference templates for auto-picking, ~500 ribosomal particles were manually selected from the micrographs and subjected to unsupervised two-dimensional (2D) classification into ten classes. Classes depicting 80S ribosomes were used for auto-picking, resulting in 162,886 particles from dataset 1, and 81,482 particles from dataset 2. The particles were extracted at a pixel size of 4.28 Å in boxes of 128 × 128 pixels and subjected to 3D classification using a human 80S ribosome filtered to 40 Å resolution as an initial reference. Only ribosomal classes depicting high-resolution structural features were retained for further processing, yielding 70,414 and 73,759 particles from dataset 1 and dataset 2, respectively. A second round of 3D classification was focused on the polypeptide exit tunnel to enrich ribosomal particles with clear density for Ebp1 and ES27L, yielding 21,743 and 55,177 particles from the first and second dataset, respectively. For this purpose, we generated a binary mask encompassing only Ebp1 and ES27L from one of the 3D classes via manual segmentation of the density in UCSF Chimera48 and used it as a reference mask during 3D classification. Sampling was switched off during this second round of 3D classification, and optimal translations and rotation from the first round of 3D classification were used for each particle. The retained particles were re-centered, extracted at a pixel size of 1.52 Å in boxes of 360 × 360 pixels and subjected to 3D auto-refinement. After 3D auto-refinement, particles were subjected to CTF refinement using standard parameters (plus dataset-wise beam tilt estimation) and Bayesian particle polishing49 using a training set of 5000 particles.
At this point, we joined polished particles from both data sets and subjected them to 3D auto-refinement. In the next step, a third round of 3D classification was focused on Ebp1–ES27L, again keeping particle positions and orientations fixed according to optimal parameters determined during the preceding 3D auto-refinement run. The remaining 34,467 particles with optimal density for Ebp1–ES27L were selected and subjected to a final round of 3D auto-refinement, which served as basis for 3D multibody refinement. In a first run, we split the 80S–Ebp1 density into two separate segments (“2-body”), comprising the 40S ribosomal subunit and the 60S ribosomal subunit plus Ebp1, respectively, to compensate for intersubunit rotation of the ribosomal subunits. This approach resulted in density segments at 3.55 Å (40S) and 3.3 Å global resolution (60S-Ebp1) after post-processing using the subunit masks also used for multibody refinement. Lower local resolution and smeared out density for peripheral regions of Ebp1 and ES27L density segments suggested conformational mobility of Ebp1 and ES27L independent from the 60S subunit. We thus subjected particles to a second round of 3D multibody refinement, in which we split the 80S–Ebp1 complex into three independently moving segments (“3-body”): the 40S subunit, the 60S subunit and the Ebp1–ES27L segment. This resulted in density segments at 3.55 Å (40S), 3.3 Å (60S), and 5.7 Å global resolution (Ebp1–ES27L) after post-processing using the masks also used for multibody refinement. Interpretable features in particular for ES27L greatly improved using this approach.
In an attempt to improve resolution of the 60S ribosomal subunit for H59 model building, we repeated the initial processing steps for the pre-selected particles extracted at full spatial resolution (1.07 Å pixel size, box size of 512 × 512 pixels). After 2-body multibody refinement, global resolution of the 60S-Ebp1 segment after post processing was comparable (3.3 Å), but interpretability of the density improved because of the finer sampling.
For analysis of ES27L conformational mobility, we prepared a binary mask including exclusively the ES27L density projecting from the ribosomal core towards Ebp1 and used it for a focused 3D classification of the joined set of 76,920 particles. Again, particle positions and orientations were kept fixed according to optimal parameters determined during the preceding 3D auto-refinement run. Three out of the ten resulting classes represented well-defined states of ES27L in slightly different conformations. Particles belonging to these classes were selected and independently subjected to one round of 3D auto-refinement and 2-body multibody refinement. After post-processing, global resolution of the 60S-Ebp1 density segment was estimated to be approximately 4.5 Å for all three classes, but resolution for ES27L was clearly lower and the density maps were filtered according to local resolution for interpretation.
All resolution estimates were performed according to the “gold standard” FSC criterion of independently refined half maps (FSC = 0.143) within RELION. Local resolution was estimated using RELION’s local post-processing implementation. The B-factor values used during local resolution filtering were guided by B-factors fitted during post processing of the various density segments, and ranged from −50 to −200 Å2.
Atomic model building and refinement
As starting point, the high-resolution cryo-EM structure of the human 80S ribosome solved at 2.9 Å resolution22 (PDB ID: 6EK0) was rigid body fitted into the cryo-EM density map, computed at full spatial resolution in UCSF Chimera48. All further atomic model building was then performed in COOT50. The 1.6 Å Ebp1 X-ray structure5 (PDB ID: 2Q8K) was manually transferred into the corresponding cryo-EM density at the ribosomal tunnel exit and real space refined by rigid-body placement. Restrained real-space refinement and validation was performed with the PHENIX suite51,52. In a first step, we optimized the fit for Ebp1 and its conformationally unchanged contact partners within the inner ring of the tunnel exit (eL19, uL23, uL24, and uL29 as well as the 5.8S rRNA (helix 24) and the 28S rRNA (helices H24, H47, H53)). In a second step, helix H59 of 28S rRNA was remodeled and refined together with the already refined model. For model building of the contact site between Ebp1 and ES27L-B, we used the cryo-EM density obtained after “3-body” refinement. An ideal A-RNA double helix was placed in the ES27L-B region interacting with Ebp1, and GA and UG mismatches were manually optimized for favorable hydrogen bonding at their Watson–Crick edges. The sequence register was defined by a combination of structural information for ES27L-B from yeast25, apparent bulging of A3252 and the defined distance to ES27L-B emanation from the previously built and well-defined ES27L-A22. The C-terminal helical extension of Ebp1 was subsequently built in the rod-shaped density within the widened major groove of ES27L-B, and the sequence register was deduced from the adjacent C-terminus of the Ebp1 X-ray structure. Finally, the remainder of ES27L-B towards ES27L-A was built accordingly to the ES27L–Ebp1 interaction region into one of the cryo-EM reconstructions obtained after 3D classification focused on ES27L, and the whole model was subjected to a final round of refinement and validation in PHENIX. Figures were prepared with programs UCSF Chimera48 and PyMOL53.
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
The following cryo-EM densities for the Ebp1–ribosome complex have been deposited in the EMDataBank: Ebp1-60S segment at full spatial resolution from 2-body multibody refinement (EMD-10344); Ebp1–ES27L segment from 3-body multibody refinement (EMD-10609); Ebp1-60S segment from 2-body multibody refinement after sorting for ES27L conformation (EMD-10608). The atomic coordinates for Ebp1 and interacting ribosomal components have been deposited in the RCSB with accession ID 6SXO. For visualization of the model in context of the entire human 80S ribosome, we recommend to superpose our atomic coordinates to the structure of the human ribosome solved at 2.9 Å resolution (PDB ID 6EK0)22, which is virtually identical except for the Ebp1-interacting region. Other data are available from the corresponding authors upon reasonable request.
Nguyen, D. Q. et al. The role of ErbB3 binding protein 1 in cancer: friend or foe? J. Cell Physiol. 233, 9110–9120 (2018).
Yoo, J. Y. et al. Interaction of the PA2G4 (EBP1) protein with ErbB-3 and regulation of this binding by heregulin. Br. J. Cancer 82, 683–690 (2000).
Pilipenko, E. V. et al. A cell cycle-dependent protein serves as a template-specific translation initiation factor. Genes Dev. 14, 2028–2045 (2000).
Squatrito, M., Mancino, M., Sala, L. & Draetta, G. F. Ebp1 is a dsRNA-binding protein associated with ribosomes that modulates eIF2alpha phosphorylation. Biochem. Biophys. Res. Commun. 344, 859–868 (2006).
Kowalinski, E. et al. The crystal structure of Ebp1 reveals a methionine aminopeptidase fold as binding platform for multiple interactions. FEBS Lett. 581, 4450–4454 (2007).
Monie, T. P. et al. Structural insights into the transcriptional and translational roles of Ebp1. EMBO J. 26, 3936–3944 (2007).
Lowther, W. T. & Matthews, B. W. Structure and function of the methionine aminopeptidases. Biochim. Biophys. Acta 1477, 157–167 (2000).
Ball, L. A. & Kaesberg, P. Cleavage of the N-terminal formylmethionine residue from a bacteriophage coat protein in vitro. J. Mol. Biol. 79, 531–537 (1973).
Kramer, G., Shiber, A. & Bukau, B. Mechanisms of cotranslational maturation of newly synthesized proteins. Annu Rev. Biochem. 88, 337–364 (2019).
Zhang, Y., Sinning, I. & Rospert, S. Two chaperones locked in an embrace: structure and function of the ribosome-associated complex RAC. Nat. Struct. Mol. Biol. 24, 611–619 (2017).
Karlsson, T., Altankhuyag, A., Dobrovolska, O., Turcu, D. C. & Lewis, A. E. A polybasic motif in ErbB3-binding protein 1 (EBP1) has key functions in nucleolar localization and polyphosphoinositide interaction. Biochem. J. 473, 2033–2047 (2016).
Bradatsch, B. et al. Arx1 functions as an unorthodox nuclear export receptor for the 60S preribosomal subunit. Mol. Cell 27, 767–779 (2007).
Greber, B. J., Boehringer, D., Montellese, C. & Ban, N. Cryo-EM structures of Arx1 and maturation factors Rei1 and Jjj1 bound to the 60S ribosomal subunit. Nat. Struct. Mol. Biol. 19, 1228–1233 (2012).
Greber, B. J. et al. Insertion of the biogenesis factor rei1 probes the ribosomal tunnel during 60S maturation. Cell 164, 91–102 (2016).
Bradatsch, B. et al. Structure of the pre-60S ribosomal subunit with nuclear export factor Arx1 bound at the exit tunnel. Nat. Struct. Mol. Biol. 19, 1234–1241 (2012).
Bhakta, S., Akbar, S. & Sengupta, J. Cryo-EM structures reveal relocalization of MetAP in the presence of other protein biogenesis factors at the ribosomal tunnel exit. J. Mol. Biol. 431, 1426–1439 (2019).
Fujii, K., Susanto, T. T., Saurabh, S. & Barna, M. Decoding the function of expansion segments in ribosomes. Mol. Cell 72, 1013–1020 e6 (2018).
Parks, M. M. et al. Variant ribosomal RNA alleles are conserved and exhibit tissue-specific expression. Sci. Adv. 4, eaao0665 (2018).
Sweeney, R., Chen, L. & Yao, M. C. An rRNA variable region has an evolutionarily conserved essential role despite sequence divergence. Mol. Cell Biol. 14, 4203–4215 (1994).
Anger, A. M. et al. Structures of the human and Drosophila 80S ribosome. Nature 497, 80–85 (2013).
Khatter, H., Myasnikov, A. G., Natchiar, S. K. & Klaholz, B. P. Structure of the human 80S ribosome. Nature 520, 640–645 (2015).
Natchiar, S. K., Myasnikov, A. G., Kratzat, H., Hazemann, I. & Klaholz, B. P. Visualization of chemical modifications in the human 80S ribosome structure. Nature 551, 472–477 (2017).
Beckmann, R. et al. Architecture of the protein-conducting channel associated with the translating 80S ribosome. Cell 107, 361–372 (2001).
Becker, T. et al. Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome. Science 326, 1369–1373 (2009).
Knorr, A. G. et al. Ribosome-NatA architecture reveals that rRNA expansion segments coordinate N-terminal acetylation. Nat. Struct. Mol. Biol. 26, 35–39 (2019).
Battiste, J. L. et al. Alpha helix-RNA major groove recognition in an HIV-1 rev peptide-RRE RNA complex. Science 273, 1547–1551 (1996).
Grotwinkel, J. T., Wild, K., Segnitz, B. & Sinning, I. S. R. P. RNA remodeling by SRP68 explains its role in protein translocation. Science 344, 101–104 (2014).
Kramer, G., Boehringer, D., Ban, N. & Bukau, B. The ribosome as a platform for co-translational processing, folding and targeting of newly synthesized proteins. Nat. Struct. Mol. Biol. 16, 589–597 (2009).
Merz, F. et al. Molecular mechanism and structure of Trigger Factor bound to the translating ribosome. EMBO J. 27, 1622–1632 (2008).
Ferbitz, L. et al. Trigger factor in complex with the ribosome forms a molecular cradle for nascent proteins. Nature 431, 590–596 (2004).
Kramer, G. et al. L23 protein functions as a chaperone docking site on the ribosome. Nature 419, 171–174 (2002).
Halic, M. et al. Structure of the signal recognition particle interacting with the elongation-arrested ribosome. Nature 427, 808–814 (2004).
Pool, M. R., Stumm, J., Fulga, T. A., Sinning, I. & Dobberstein, B. Distinct modes of signal recognition particle interaction with the ribosome. Science 297, 1345–1348 (2002).
Voorhees, R. M. & Hegde, R. S. Structures of the scanning and engaged states of the mammalian SRP-ribosome complex. Elife 4, e07975 (2015).
Gogala, M. et al. Structures of the Sec61 complex engaged in nascent peptide translocation or membrane insertion. Nature 506, 107–110 (2014).
Voorhees, R. M. & Hegde, R. S. Structure of the Sec61 channel opened by a signal sequence. Science 351, 88–91 (2016).
Gamerdinger, M. et al. Early scanning of nascent polypeptides inside the ribosomal tunnel by NAC. Mol. Cell 5, 996–1006 e8 (2019).
Blau, M. et al. ERj1p uses a universal ribosomal adaptor site to coordinate the 80S ribosome at the membrane. Nat. Struct. Mol. Biol. 12, 1015–1016 (2005).
Kisly, I. et al. The functional role of eL19 and eB12 intersubunit bridge in the eukaryotic ribosome. J. Mol. Biol. 428, 2203–2216 (2016).
Liu, Z., Ahn, J. Y., Liu, X. & Ye, K. Ebp1 isoforms distinctively regulate cell survival and differentiation. Proc. Natl Acad. Sci. USA 103, 10917–10922 (2006).
Bhat, M. et al. Targeting the translation machinery in cancer. Nat. Rev. Drug Disco. 14, 261–278 (2015).
Studier, F. W. Protein production by auto-induction in high density shaking cultures. Protein Expr. Purif. 41, 207–234 (2005).
Wild, K. et al. Reconstitution of the human SRP system and quantitative and systematic analysis of its ribosome interactions. Nucleic Acids Res. 47, 3184–3196 (2019).
Khatter, H. et al. Purification, characterization and crystallization of the human 80S ribosome. Nucleic Acids Res. 42, e49 (2014).
Zivanov, J. et al. New tools for automated high-resolution cryo-EM structure determination in RELION-3. eLife 7, e42166 (2018).
Zheng, S. Q. et al. MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Nat. Methods 14, 331–332 (2017).
Zhang, K. Gctf: real-time CTF determination and correction. J. Struct. Biol. 193, 1–12 (2016).
Pettersen, E. F. et al. UCSF Chimera–a visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605–1612 (2004).
Zivanov, J., Nakane, T. & Scheres, S. H. W. A Bayesian approach to beam-induced motion correction in cryo-EM single-particle analysis. IUCrJ 6, 5–17 (2019).
Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr. D. Biol. Crystallogr. 66, 486–501 (2010).
Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr. D. Biol. Crystallogr. 66, 213–221 (2010).
Afonine, P. V. et al. Real-space refinement in PHENIX for cryo-EM and crystallography. Acta Crystallogr. D. Struct. Biol. 74, 531–544 (2018).
Schrodinger, L. L. C. The PyMOL Molecular Graphics System, Version 2.3.1. (2019). https://pymol.org/2/.
Liu, S., Widom, J., Kemp, C. W., Crews, C. M. & Clardy, J. Structure of human methionine aminopeptidase-2 complexed with fumagillin. Science 282, 1324–1327 (1998).
We acknowledge access to the infrastructure of the Cryo-EM Network at the Heidelberg University and support by Götz Hofhaus (Bioquant). We acknowledge the services SDS@hd and bwHPC supported by the Ministry of Science, Research and the Arts Baden-Württemberg, as well as the German Research Foundation (INST 35/1314-1 FUGG and INST 35/1134-1 FUGG). This work was supported by the Deutsche Forschungsgemeinschaft through the Leibniz Programme (SI 586/6-1) and the SFB 1036 (TP22) to I.S. I.S. is an investigator of the Cluster of Excellence:CellNetworks.
The authors declare no competing interests.
Peer review information Nature Communications thanks Jayati Sengupta and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Wild, K., Aleksić, M., Lapouge, K. et al. MetAP-like Ebp1 occupies the human ribosomal tunnel exit and recruits flexible rRNA expansion segments. Nat Commun 11, 776 (2020). https://doi.org/10.1038/s41467-020-14603-7
This article is cited by
PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner
Cell & Bioscience (2022)
Chemical reversible crosslinking enables measurement of RNA 3D distances and alternative conformations in cells
Nature Communications (2022)
High-resolution structures of a thermophilic eukaryotic 80S ribosome reveal atomistic details of translocation
Nature Communications (2022)
Communications Biology (2021)
The roles of multifunctional protein ErbB3 binding protein 1 (EBP1) isoforms from development to disease
Experimental & Molecular Medicine (2020)