A MAFG-lncRNA axis links systemic nutrient abundance to hepatic glucose metabolism


Obesity and type 2 diabetes mellitus are global emergencies and long noncoding RNAs (lncRNAs) are regulatory transcripts with elusive functions in metabolism. Here we show that a high fraction of lncRNAs, but not protein-coding mRNAs, are repressed during diet-induced obesity (DIO) and refeeding, whilst nutrient deprivation induced lncRNAs in mouse liver. Similarly, lncRNAs are lost in diabetic humans. LncRNA promoter analyses, global cistrome and gain-of-function analyses confirm that increased MAFG signaling during DIO curbs lncRNA expression. Silencing Mafg in mouse hepatocytes and obese mice elicits a fasting-like gene expression profile, improves glucose metabolism, de-represses lncRNAs and impairs mammalian target of rapamycin (mTOR) activation. We find that obesity-repressed LincIRS2 is controlled by MAFG and observe that genetic and RNAi-mediated LincIRS2 loss causes elevated blood glucose, insulin resistance and aberrant glucose output in lean mice. Taken together, we identify a MAFG-lncRNA axis controlling hepatic glucose metabolism in health and metabolic disease.


Cellular and organism-level energy homeostasis and nutrient partitioning are instrumental for survival. In higher organisms, multi-organ systems evolved to react to shifts in energy supply by storing (anabolic) or metabolizing (catabolic) nutrients, for instance by conversion of simple sugars to storage macromolecules like glycogen or by glucose catabolism, according to caloric demands1. A key process for mounting appropriate responses to altered nutrient availabilities are abundance, localization, and nutrient-induced activation of transcriptional networks that couple energy states to appropriate changes in gene expression2. These ancient molecular circuits ensured survival of animals during (historically frequent) food shortages. In contrast, the exposure to constant calorie overload, coupled with sedentary lifestyles, results in excess storage of nutrients, and concomitantly, obesity and obesity-associated maladies. Globally, two billion individuals are considered overweight or obese and exhibit elevated risks of developing severe comorbidities such as cardiovascular disease3, artherosclerosis4, type 2 diabetes mellitus (T2D), liver steatosis, or nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH)5. Therefore, understanding how nutrient-sensitive signaling networks are controlled during conditions of energy surplus, and whether this can be prevented in obesity, will be instrumental in designing effective therapies in the future.

Remarkable for living in seemingly postgenomic times, we currently witness a paradigm shift in understanding our genomes and the information contained therein: multinational sequencing efforts like ENCODE6, FANTOM7, or NONCODE8, together with increasing RNA-Sequencing (RNA-Seq) capabilities and reduced costs, led to the intriguing discovery that, whereas only 1–2% of genomic information encodes protein-coding mRNAs, more than 70% of DNA is transcribed across developmental space and time9,10. This led to the identification of thousands of microRNAs, and more recently, long noncoding RNAs (lncRNAs)11,12 in mice and humans13. The identification of these many lncRNAs in silico and ascribing a specific function to each has been challenging14. Functions identified to date include microRNA scavenging15, chromatin sequestration16, 3D genome organization17, chromatin modifier recruitment18, lncRNA–DNA triplex formation19, and small-molecule protein complex formation20 to control mRNA translation15 and RNA–RNA crosstalk21.

Investigators have only begun to ascribe specific in vivo functions for lncRNAs in cellular energy homeostasis and/or metaboregulatory signaling circuits. Such functions of lncRNAs include control of hepatic cholesterol biosynthesis22, de novo lipogenesis and systemic lipid homeostasis23,24, beta-oxidation25, glucose homeostasis26,27, and regulation of insulin sensitivity in the liver27. Understanding the molecular details of these regulatory processes may lead to innovative approaches where repression of specific lncRNAs may prove useful in the treatment of metabolic diseases.

By performing RNA-Seq in mouse models for dietary and genetic obesity, T2D, and livers from diabetic human patients, we find that nutrient excess and refeeding (RF) favors lncRNA repression, whereas fasting induces lncRNA expression. Intriguingly, protein-coding mRNAs are not affected accordingly. In silico analyses of lncRNA and mRNA promoters, and integration of MAFG ChIP-Seq with MAFG gain-of-function RNA-Seq data confirm that elevated MAFG signaling in obesity and RF is transcriptionally linked to lncRNA repression. In vitro and in vivo loss of MAFG in hepatocytes controls glucose production, improves glucose metabolism during obesity, and induces lncRNAs, whereas MAFG gain of function represses hepatic lncRNAs. Intriguingly, MAFG loss prevents insulin-evoked activation of mTORC1 signaling, thus presumably interfering with protein translation in hepatocytes. We further observe that obesity-repressed LincIRS2 is negatively controlled by MAFG and CRISPR–Cas9-mediated knockout, or antisense-mediated RNA interference of LincIRS2 causes hyperglycemia, insulin resistance, likely caused by alterations in glucogenic gene expression in lean mice.


Nutrient states elicit opposing effects on mRNA and lncRNAs

To identify lncRNAs that are implicated in the development of liver disease pathologies in diet-induced obesity (DIO), for instance insulin resistance, steatosis, and liver inflammation, 6-week-old C57BL/6N mice were fed a high-fat diet (HFD) or control diet (CD). After 30 weeks, hepatic RNA was isolated and total RNA-Sequencing (RNA-Seq) performed. This approach identified 583 mRNAs and 50 lncRNAs that were significantly (p value (pV) < 0.05 by Student’s t test, false-discovery rate < 0.05, and CuffDiff function significant = “yes” for Benjamini–Hochberg correction for multiple testing) altered after HFD feeding (Fig. 1a, Supplementary Data 1). Performing Ingenuity Pathway Analysis (IPA) confirmed activation of transcription factor (TF) networks and signaling pathways known to be induced in the liver under anabolic/nutrient-rich conditions. These included insulin receptor (IR), Forkhead Box O1 (FOXO1), and Sterol Regulatory Element Binding Transcription Factor 1C (SREBP1C) pathways (Supplementary Fig. 1a–c). We interpreted these transcriptional changes as a reflection of chronic nutrient exposure that in turn triggers anabolic TF pathways in the liver.

Fig. 1: Systemic nutrient states elicit opposing effects on liver mRNA and lncRNA expression.

a, b Histogram plot of read counts (top), scatter plot of reads counts vs. log2-transformed expression ratios (Log2R, bottom) and c, d cumulative frequency distribution (Cf) of Log2R of hepatic protein-coding mRNA (blue) and lncRNA (orange) expression changes. Data are from total RNA-Sequencing (RNA-Seq) in the liver of C57BL/6N mice after a, c HFD vs. (vs) CD feeding (n = 3 each) or b, d ad libitum feeding (AL) vs. 16 h of fasting (FA) (n = 4 each). e Log2R Cf of mRNA (blue) and lncRNA (orange) expression changes in the liver from lean (L) vs. obese (OB) patients without T2D (n = 4 per group). f Log2R Cf of mRNA (blue) and lncRNA (orange) expression changes in the liver of lean (L) vs. obese patients with T2D (n = 4 per group). gj Quantitative reverse-transcription (RT) polymerase chain reaction (qPCR) for L-DIO-lncRNA expression in mice exposed to g HFD vs. CD feeding (n = 4–6). h Leprdb vs. Dock7m (n = 4). i AL vs. FA (n = 5 each) and j RF vs. FA (n = 4 each). cf Statistical differences between mRNAs and lncRNAs were assessed using nonparametric Kolgomorov–Smirnov (KS) tests. p Values are given in the panels. Bar graphs represent mean ± s.e.m. with all data points plotted and differences in (gj) were calculated using unpaired, two-tailed Student’s t tests. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file. Icons in af were created with BioRender.com.

When performing differential gene expression (DGE) analysis independently for protein-coding or lncRNA transcripts (gene biotypes from Ensembl Biomart28), we observed a significant cumulative downregulation of lncRNAs as compared with protein-coding mRNAs (Fig. 1c) in obese livers using nonparametric Kolmogorov–Smirnov tests. Prompted by this difference in mRNA vs. lncRNA regulation after HFD feeding, we repeated the analysis after physiological nutrient changes outside of obesity and metabolic disease. Comparing transcriptomes from ad libitum (AL)-fed C57BL/6N (wild-type) mice compared with mice fasted for 16 h (FA, Fig. 1b, Supplementary Data 2), we observed 1165 mRNAs and 92 lncRNAs differentially regulated. In contrast to lncRNA repression in obesity, lncRNAs were induced after fasting when compared with mRNAs (Fig. 1d). Short-term (6 h) RF altered 587 mRNAs and 59 lncRNAs (Supplementary Data 3) with similar trends for induction of lncRNAs as compared with mRNAs (Supplementary Fig. 1d). We performed RNA-Seq in BKS.Cg-Dock7m+/+; LepRdb/J (termed LepRdb) mice, a genetic model of obesity and T2D, and observed 959 mRNAs and 83 lncRNAs significantly changed (Supplementary Data 4). Again, hepatic lncRNAs were decreased compared with mRNAs (Supplementary Fig. 1e) when we compared LepRdb vs. genetic background-matched misty (Dock7m) mice, although to a lesser degree than in DIO. To determine whether similar patterns are observed in human subjects, we analyzed RNA-Seq data from liver biopsies from a cohort of lean, obese (without T2D), and obese, T2D patients (n = 4 per group)29. Consistent with our mouse studies, obesity and T2D in humans were associated with repressions in lncRNAs vs. mRNAs (Fig. 1e, f and Supplementary Data 5).

To determine if our metabolically regulated lncRNAs represent liver-enriched transcripts, we performed RNA-Seq in seven tissues of lean C57BL/6N mice and identified clusters of lncRNAs enriched in each tissue (Supplementary Fig. 1f). Within the liver-enriched cluster, seven DIO-associated lncRNAs (termed L-DIO-lncRNAs), including 4833411C07Rik, 9030616G12Rik, 9030622O22Rik, B930025P03Rik, Gm10319, Gm8883, and previously reported lncLSTR24 (Supplementary Fig. 1g) matched our criteria: the expression of L-DIO-lncRNA expression was confined to hepatocytes compared with non-parenchymal liver cell types (Supplementary Fig. 1h). With the exception of Gm8883, L-DIO-lncRNAs were located in the nucleus (Supplementary Fig. 1i), and with exception of Gm10319, exhibited low protein-coding potential (using two independent algorithms, CPAT30 and CPC14, Supplementary Fig. 1j, k). Using quantitative polymerase chain reaction (qPCR) we confirmed L-DIO-lncRNAs to be reduced by HFD vs. CD feeding (Fig. 1g), in LepRdb vs. Dock7m (Fig. 1h), in AL vs. FA (Fig. 1i), and in RF vs. FA mice (Fig. 1j). Thus, our extensive RNA-Seq analyses and qPCR validation identified an inverse correlation of nutrient levels with lncRNAs across several mouse models of altered energy homeostasis and metabolically compromised humans and identified metabolically regulated (liver-selective) lncRNAs.

Liver MAFG links high nutrient states to lncRNA repression

Our data suggested that many lncRNAs are discordantly affected by (patho)-physiological changes in nutrient states when compared with mRNAs. We hypothesized that these differences between mRNAs and lncRNAs could reflect differences in TF- binding site (TFBS) occurence in promoters of both gene sets. These differences in promoter architecture could, in turn, be differentially transactivated by nutrient-sensitive signaling pathways, leading to anticorrelative regulation of lncRNAs vs. mRNAs during obesity and T2D as observed. Our hypothesis built on in silico analyses of chromatin-state maps31 and validation studies in human cell lines32 that suggest preexisting promoter differences between lncRNAs and mRNAs. To identify TF pathways that control lncRNAs and mRNAs via distinct regulatory programs, we first analyzed putative promoter sequences (−800 bp to +100 bp around transcriptional start sites, TSS) from an extended set of 1920 mRNAs and 149 lncRNAs affected by HFD (p value < 0.1, CuffDiff DGE output). Next, we used AME33 (MEME suite34) to call differences in TF motif occurence between lncRNA and mRNA promoters. Consistent with previous reports31,35, we observed that CpG-rich motifs were overrepresented in mRNA promoters, particularly motifs recognized by the E2F family of TF (e.g., E2F2–E2F4). In contrast, lncRNA promoters were enriched for MAFG:NFE2L1 (V-Maf Avian Musculoaponeurotic Fibrosarcoma Oncogene Homolog G:Nuclear Factor, Erythroid 2 Like 1) motifs (Fig. 2a). This finding was consistent with reports demonstrating that TFBS recognized by MAFG or other members of the small MAF (smMAF) TF family (MAFF, MAFG, and MAFK) is overrepresented in lncRNA promoters31,32,35. In a second step and given the reported gene-repressive properties of MAFG homodimers36,37, we wanted to exclude that MAFG motifs occurred in lncRNA promoters simply because lncRNA tends to be more repressed than mRNAs during DIO. We thus performed de novo TF motif enrichment analyses of the putative promoter sequences (−800/+100 bp around TSS) in HOMER38 separately for induced and repressed genes, and confirmed that smMAF motifs were enriched in lncRNA promoters affected by DIO vs. protein-coding RNAs in both gene sets (Supplementary Fig. 2a, b). Taken together, our data suggested that MAFG, or other smMAF TFs, is enriched in lncRNA promoters as compared with mRNAs, and could elicit anticorrelative transcriptional effects, for instance repress hepatic lncRNAs while, at the same time, inducing levels of specific mRNAs.

Fig. 2: Obesity-associated increase in MAFG signaling links nutrient states to lncRNA repression.

a Illustration of transcription factor-binding site (TFBS) analysis and a list of TFBS enriched in promoters of mRNAs (blue) or lncRNAs (orange) affected by HFD vs. CD feeding (n = 3 each). TFBS is from UniProbe (U) or Jaspar (J). b qPCR of smMAF (Maff, Mafg, and Mafk) expression in C57BL/6N and Dock7m mice (n = 4 each). c Published BLRP ChIP-seq in livers of mice transduced with adenovirus (Ad)-overexpressing BLRP-MAFG fusion proteins (GEO ID: GSE77559)40 was used as a proxy for MAFG cistromes in the liver. BLRP-MAFG peaks per gene were determined in a ±50-kb window around the TSS (defined as ±1 bp from gene start) of expressed, induced, or repressed lncRNAs by HFD using window function in bedtools75. d qPCR of L-DIO-lncRNAs and Mafg mRNA in livers of Ad-MAFG vs. Ad-CMV mice (n = 5 each). e qPCR of lncRNA exhibiting MAFG binding within TSS ± 50 kb, as identified by BLRP-MAFG ChIP-Seq and Mafg expression after transfection of primary hepatocytes with 100 nM Mafg or Scr LNAs, n = 4–5 experiments, each performed in triplicates. f UCSC Genome Browser with expression of selected lncRNAs (“RNA-Seq”) and BLRP-MAFG binding (“ChIP-Seq” from Vallim et al.40) in the liver. g Motif activity of smMAF and MAFA TFBS across nutritional states and HFD feeding analyzed using ISMARA41 (n = 3–4 per group). h Venn diagram illustrating DGE overlap and enriched gene ontology (GO) categories shared between indicated nutritional states, diets, and genotypes. i Log2R Cf of mRNA (blue) and lncRNA (orange) expression changes in livers of Ad-MAFG vs. Ad-CMV mice (n = 4 each). d, e Bar graphs represent mean ± s.e.m. with all data points plotted, and statistical differences were calculated using d unpaired or e paired, two-tailed Student’s t test. h Significance of gene overlaps was calculated by using a hypergeometric distribution: *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file. Illustration in (a) was created with BioRender.com.

Because smMAF TF is considered as functionally redundant39, we next asked which smMAF constituted the prevalent liver isoform, and presumably, exerted the strongest effect on lncRNA repression during obesity. Using RNA-Seq, we found that Mafg accounted for the majority of smMAF transcripts in livers from lean C57BL/6N and Dock7m mice (Fig. 2b). Integrating RNA-Seq analysis from mice injected with Mafg cDNA-expressing adenoviruses (Ad-MAFG), and public MAFG chromatin immunoprecipitation coupled to sequencing (ChIP-Seq) datasets40, we found that MAFG preferentially bound lncRNAs repressed during DIO (Fig. 2c). We performed qPCR in livers from Ad-MAFG-treated mice and confirmed that the seven L-DIO-lncRNAs (Fig. 2d) were repressed after Mafg overexpression. Conversely, locked nucleic acid (LNA)-mediated knockdown of Mafg (Supplementary Fig. 2c), but not Maff or Mafk (Supplementary Fig. 2c–e), in primary hepatocytes derepressed a set of lncRNAs containing MAFG-binding sites (Fig. 2e) as determined by ChIP-Seq (Fig. 2f). These data supported the hypothesis that MAFG represses specific lncRNAs in DIO, suggesting MAFG as TF whose activity changes during metabolic states. To investigate the degree of smMAF signaling activity in lean and obese livers, we applied ISMARA41, a web-based tool modeling genome-wide expression changes in RNA-Seq data by predicting the underlying combination of TFBS. As proof of principle and as expected, we found an induction of inflammatory CEBPB and RELA-REL-NFKB1 gene programs in obese liver (Supplementary Fig. 2f, g). When comparing all combinations of nutrient-rich (HFD, AL, and RF) vs. nutrient-poor conditions (CD and FA) we found that anabolic conditions exhibited higher smMAF (NFE2:BACH1:MAFK, MAFG:NFE2L1, and MAFF) motif activities in the liver, while large MAFA42-dependent TFBS activities were reduced after DIO or RF (Fig. 2g). Importantly, RNA-Seq from HFD and Ad-MAFG-treated mice revealed a significant overlap of directional gene expression changes and overlapping gene categories (Fig. 2h, left), while Ad-MAFG and fasting gene sets showed anticorrelative trends (Fig. 2h, right). Finally, RNA-Seq in Ad-MAFG livers revealed trends toward repression of lncRNAs compared with mRNAs (Fig. 2i, Supplementary Data 6). Thus, our data suggested that acute (RF and AL) and chronic (DIO) nutrient exposure is associated with increased smMAF signaling, and that gain of function of the most abundant smMAF isoform in the liver, MAFG, resulted in preferential repression of lncRNAs in the liver.

Hepatic MAFG links energy states to glucose metabolism

SmMAFs recruit Cap’n’Collar protein like NFE2L1/2 (or NRF1/2) to antioxidant response elements of xenobiotic enzymes43 to govern bile acid homeostasis40 and hepatic lipid and amino acid metabolism44. To determine if MAFG is required to mediate metabolic changes during DIO, we first used an in vitro approach: using LNA transfection, we silenced Mafg in primary hepatocytes and observed robust suppression of Mafg mRNA and protein (Fig. 3a). To mimic fasting or RF in vitro, we treated cells with Forskolin plus Dexamethasone (FD) or FD combined with insulin (Ins, termed FDI), as reported45. At baseline, silencing Mafg increased glucose production (Fig. 3b). Treating cells with FD increased glucose production as expected, yet increases in glucose production remained higher when Mafg was silenced, suggesting increased gluconeogenesis. Importantly, no in vitro differences in hepatic insulin sensitivity were observed, as insulin treatment reduced glucose production in control (Scr) and Mafg LNA- treated cells to similar extents (Fig. 3b). In line with increased glucose production, Mafg RNAi increased basal gluconeogenic gene expression as evidenced by elevated fructose-bisphosphatase 1 (Fbp1), glucose-6-phosphatase catalytic subunit (G6pc), and phosphoenolpyruvate carboxykinase 1 (Pck1) expression, and when cells were stimulated with FD, Fbp1 and G6pc remained markedly increased (Fig. 3c). This increased glucogenic tone was confirmed by silencing MAFG using Mafg or control Scr LNAs in C57BL/6N mice. MAFG RNAi increased relative glucose production during a PTT, providing in vivo evidence of elevated gluconeogenesis (Fig. 3d). Importantly, LNA-mediated silencing of other smMAFs (Maff and Mafk) did not increase glucogenic gene expression (Supplementary Fig. 2h–j), emphasizing specific roles for MAFG in controlling hepatic glucose output. In line with unaltered suppression of glucose production (Fig. 3b), insulin-mediated suppression of gluconeogenic enzymes under basal but also FD-stimulated conditions was unchanged after Mafg loss, again supporting the notion that Mafg loss of function does not affect insulin sensitivity (Fig. 3e). Consequently, when performing Western Blot analysis in insulin-stimulated hepatocytes in the presence or absence of Mafg, we observed modest reductions in phosphorylation of serine 473 residue of serine/threonine kinase AKT/PKB (Fig. 3f), yet reduced phosphorylation of the serine 9 of glycogen synthase kinase 3 beta (GSK3B, Supplementary Fig. 2k), which is dispensible for HGP46. This suggested that IR-proximal kinases controlling glucose output, but not more distal events, are not affected by Mafg loss.

Fig. 3: MAFG signaling in hepatocytes represses glucose production and supports mTOR activation.

a Representative MAFG immunoblot and Mafg expression after transfection of primary hepatocytes with 100 nM Mafg or Scr LNA. b Relative glucose levels in primary hepatocytes after transfection with 100 nM Mafg or Scr LNAs and stimulation for 24 h with 10 µm Fsk plus 100 nM Dex (FD) or FD combined with 100 nM insulin (FDI). c, e Indicated mRNAs after transfection with Mafg or Scr LNAs under basal conditions or stimulated with c 10 µM Fsk plus 100 nM Dex (FD) or e 100 nM insulin. Data represent a n = 5 or b, c, e n = 3–4 independent experiments, each performed in triplicates. d Pyruvate tolerance test (PTT) performed in C57BL/6N mice after 6 weeks of HFD feeding with 10 mg kg−1 of Mafg (n = 9) or Scr (n = 8) LNA injected 5 days before. fh Immunoblots of f total and Serine 473 phosphorylated AKT/PKB (pAKTS473). g Total and Serine 2448 phosphorylated mammalian target of rapamycin (pmTORS2448) and h total and Threonine 37/46 phosphorylated eukaryotic translation initiation factor 4E binding protein 1 (p4EB-BP137/46) after transfection of primary hepatocytes with 100 nM Mafg or Scr LNAs and stimulated for 10 min with vehicle (v.e.) or 100 nM insulin. Separate membranes were loaded with equal amounts of protein lysate for total and phospho-specific immunoblotting, and equal loading was confirmed by calnexin (CLNX) for each membrane. Due to different molecular weights of mTOR, PKB/AKT, and 4E-BP1, these proteins were partly investigated on the same membranes together with the same CLNX antibody. CLNXa and CLNXb bands are therefore identical. Each blot is representative of n = 3 immunoblots, each immunoblot performed after transfection of Mafg vs. Scr LNA in hepatocyte preparations from n = 2 mice. ac, e Bar graphs represent mean ± s.e.m. with all data points plotted and statistical differences were calculated using a paired, two-tailed Student’s t test. b, c, e One-way ANOVA and d two-way ANOVA with Bonferroni post correction for multiple testing. Superscripts depict group comparisons for post analysis (a–f = comparison vs. column 1–6). d Box plot bounds depict upper and lower quartiles with median as the center. Whiskers span all values: *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.

Another important TF pathway that translates information about nutrient states into increases in cell proliferation and protein translation is mammalian target of rapamycin (mTOR)47: we found insulin-evoked induction of serine 2448 mTOR phosphorylation (Fig. 3g) and concomitant increases in mTOR Complex 1 (mTORC1) activity, as evidenced by mTORC1-dependent threonine 37/46 phosphorylation of eIF4E-binding protein 1 (p4E-BP1, Fig. 3h)48, blunted after Mafg silencing, suggesting that MAFG supports mTORC1-dependent processes like ribosome biogenesis and cap-dependent translation49 after insulin stimulation. Thus, we showed that increases in MAFG signaling (e.g., in DIO and after RF) preferentially repressed lncRNAs in primary hepatocytes, while LNA-mediated loss of Mafg induced lncRNAs, elicited a fasting-like expression profile, and caused specific defects in insulin-dependent activation of signaling pathways linked to protein translation.

MAFG loss protects from obesity-induced hyperglycemia

Having identified MAFG as an important regulator of lncRNA expression, and showing that Mafg repressed glucose output in hepatocytes, we next turned to in vivo loss of function. Given the obesity-evoked increase in MAFG signaling, we hypothesized that Mafg loss in vivo could be metabolically favorable in DIO and conducted Mafg knockdown by performing biweekly injections of Mafg or Scr LNAs in CD- or HFD-fed C57BL/6N mice. Mafg treatment was well tolerated over the duration of 12 weeks and caused suppression of Mafg mRNA and protein expression in lean and obese liver (Fig. 4a, f) and kidney (Supplementary Fig. 3a), but did not affect liver Maff or Mafk expression in the liver (Supplementary Fig. 3b). When studying the metabolic consequences of Mafg silencing, we did not observe alterations in body weight (BW) in CD (Fig. 4b) or HFD-fed (Fig. 4g) mice. Mafg knockdown modestly reduced fasting blood glucose (p = 0.073), but did not have an effect on blood glucose concentrations in the fed state (Fig. 4c), improved glucose tolerance (Fig. 4d), and trended toward improved insulin sensitivity (Fig. 4e) in CD-fed mice with more pronounced effects in obese animals (Fig. 4h–j). Indirect calorimetric quantification of oxygen consumption, carbon dioxide production, respiratory exchange ratios, energy expenditure, and food intake revealed no differences between groups on either CD or HFD (Supplementary Fig. 3c–g). No histological changes between genotypes were observed concerning fat accumulation in obese livers (Supplementary Fig. 3h). Collectively, these results implicated hepatic alterations in glucose homeostasis in the observed improvement of metabolism in Mafg LNA-treated mice. When performing RNA-Seq analysis in CD-fed Scr LNA-treated vs. HFD-fed Scr LNA-treated mice, we observed 218 genes significantly altered. When we compared these gene changes to the 357 genes significantly changed by Mafg LNA vs. Scr LNA treatment in HFD-fed mice, we found n = 66 genes shared between both groups (Fig. 4k and Supplementary Data 8 and 12). Interestingly, we found that genes were induced by DIO as often reduced by Mafg LNA treatment. Particularly the DIO-associated increase in inflammatory gene categories was repressed in Mafg-deficient obese livers (Fig. 4k). These transcriptome analyses suggested that Mafg inhibition reinstated a healthy, less inflammatory expression profile in livers of obese mice, exemplified by trends toward increased serum fibroblast growth factor 21 (FGF21) levels (Fig. 4l), a hepatokine beneficial in the context of obesity in mice50. Furthermore, we observed trends (p = 0.103) toward global lncRNA derepression (Fig. 4m) as expected from Mafg RNAi in vitro.

Fig. 4: MAFG loss protects from obesity-induced hyperglycemia.

a qPCR of Mafg expression in the liver from CD-fed 21-week-old C57BL/6N mice after 15 weeks of 10 mg kg−1 of Mafg (n = 8) or Scr LNA (n = 7) and immunoblot of MAFG and CLNX protein. b Body weight, c 16-h fasted, and random-fed glucose, d GTT, e ITT in CD-fed C57BL/6N mice after 6 and 5 weeks of Mafg or Scr LNA (n = 8). f qPCR of Mafg expression in the liver from 21-week-old HFD-fed mice after 15 weeks of Mafg or Scr LNA (n = 8). g Body weight, h 16-h fasted, and random-fed glucose, i GTT, and j ITT in HFD-fed C57BL/6N mice after 6 and 5 weeks of Mafg or Scr LNA (n = 8). k DGE overlap and enriched categories in livers from CD- or HFD-fed C57BL/6N mice after 10 weeks of Mafg or Scr LNA (n = 4). l FGF21 levels from 21-week-old HFD-fed C57BL/6N mice after 15 weeks of Mafg (n = 8) or Scr LNA (n = 7). m Log2R Cf of mRNA and lncRNA expression changes in 21-week-old HFD-fed mice after 15 weeks of Mafg or Scr LNA (n = 3). n qPCR of Mafg expression in liver and indicated tissues after 20 weeks of 5 mg kg−1 Mafg or Control ASO (n = 7 Mafg and n = 10 Control ASO in liver, n = 4 other tissues) and immunoblot of MAFG and CLNX in the liver of HFD-fed C57BL/6N mice after 2 days of Mafg or Control ASO. o BW in 16-week-old HFD-fed mice after 10 weeks of Mafg or Control ASO (n = 7 Mafg and n = 10 Control ASO). Absolute p relative q, r glucose during ITT in HFD-fed p, q C57BL/6N or r Maff/Mafk−/− (DKO) mice after 11 weeks of Mafg or Control ASO (n = 6 Control ASO, n = 9 Mafg ASO, and n = 6 Mafg ASO in DKO). Bar graphs represent mean ± s.e.m. with all data plotted and statistical differences calculated using a, c, f, h, n unpaired, two-tailed and l unpaired, one-tailed Student’s t test. m Kolgomorov–Smirnov b, d, e, g, i, j, pr two-way ANOVA with Bonferroni post correction. k Significance of gene overlaps was calculated by using a hypergeometric distribution: *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.

To corroborate our findings of improved metabolic control upon Mafg silencing during obesity using independent approaches, we repeated our RNAi studies using N-acetyl galactosamine (GalNAc)-conjugated Mafg and Control antisense oligonucleotide (ASO) inhibitors. In addition, given the presence of three, presumably functionally redundant51, smMAF isoforms in the liver, we silenced Mafg in the liver of HFD-fed C57BL/6 or global MafF−/−/MafK−/− double-knockout (DKO) mice. After 2 weeks of initial ASO administration, mice were fed HFD for 18 weeks. As for LNA-evoked Mafg ablation, Mafg ASO sustainably reduced Mafg mRNA and protein not only in the liver, but also in the kidney, vWAT, and SKM (Fig. 4n), yet did not affect Maff and Mafk expression (Supplementary Fig. 3i). Also similar to our previous study by using Mafg LNAs, in the absence of overt BW changes (Fig. 4o), Mafg loss improved insulin sensitivity (Fig. 4p, q), yet no changes in glycemia, insulin, and triglyceride levels were observed (Supplementary Fig. 3j). Crucially, ablating Mafg upon preexisting Maff/Mafk deficiency resulted in further improvements in insulin sensitivity (Fig. 4r). Thus, we here demonstrate that Mafg loss using two independent RNAi approaches improved glucose metabolism and insulin sensitivity, particularly in obese mice.

LincIRS2 knockdown causes insulin resistance in lean mice

Having demonstrated that loss of MAFG not only improved glucose homeostasis and insulin sensitivity but also, concurrently, controlled lncRNA abundance in the liver, we asked if MAFG-repressed lncRNAs are implicated in liver metabolism focused on characterizing MAFG-dependent L-DIO-lncRNA 4833411C07Rik, which we refer to as “LincIRS2” due to its positioning 80 kb 5′ of Irs2 (Supplementary Fig. 4a) in the following. As expected from our previous Mafg overexpression data, LincIRS2 was induced in livers from Mafg LNA and Mafg ASO-treated C57BL/6N mice (Fig. 5a). As obesity represents a complex scenario of dysregulated glucose, insulin, lipid, and hormone homeostasis that all affect liver energy metabolism1, we investigated which obesity-related nutrients, hormonal factors, and nutrient-sensitive TFs, in addition to MAFG, regulated LincIRS2. For this, we expressed constitutively active (ca) versions of metaboregulatory TFs in primary hepatocytes, including glucose-sensing MLX-interacting protein like/carbohydrate response element-binding protein (caMLXIPL/ChREBP52), FOXO1 (caFOXO1153), or lipogenic caSREBP1C54. We found that LincIRS2 was induced upon catabolic caFOXO1 and caChREBP expression in primary hepatocytes (Fig. 5b) in addition to fasting–mimicking cyclic adenosin-3′,5′-monophosphate (cAMP) and glucagon (GCG) stimulation (Fig. 5c). On the other hand, insulin (with or without glucose co-stimulation) repressed LincIRS2 (Fig. 5d), whereas IR knockout in liver induced LincIRS2 (Fig. 5e). Concomitantly, constitutive active versions of (insulin-dependent) SREBP1C (caSREBP1) decreased LincIRS2 expression (Fig. 5f). Thus, LincIRS2 inversely correlates with MAFG and insulin signal transduction across several in vitro and in vivo models of altered signaling and nutritional states.

Fig. 5: Knockdown of MAFG-dependent LincIRS2 causes mild insulin resistance in lean mice.

a qPCR of LincIRS2 expression in the liver from C57BL/6N mice treated with Scr LNA (n = 7), Mafg LNA (n = 8), Control ASO (n = 10), or Mafg ASO (n = 7). bf qPCR for LincIRS2 expression in primary hepatocytes after b 24 h of transduction with adenoviruses (Ad)-expressing caChREBP and caFOXO1, c 16 h of 200 nM GCG and 100 µM cAMP, d 6 h of 27.5 mM glucose, and/or e 100 nM insulin in livers from mice with hepatocyte-specific IR knockout vs. Cre-negative Controls59 (n = 3). f Twenty-four hours after transduction of primary hepatocytes with Ad-caSREBP1c. g qPCR of LincIRS2 expression after transfection of primary hepatocytes with 100 nM LincIRS2 or Scr LNAs and 6 h of stimulation with g FD and GCG or h insulin. g, h n = 6–10 independent experiments, each in triplicate. i–k qPCR of Pck1 expression after transfection with 100 nM LincIRS2 or Scr LNAs and 6 h of stimulation with i insulin, j FD, or k GCG. ik n = 5–10 experiments, each in triplicates. l qPCR of LincIRS2 expression in indicated tissues from 21-week-old CD-fed mice after 15 weeks of 10 mg kg−1 LincIRS2 or Scr LNA (n = 4) treatment. m, n Body weight in m CD- and n HFD-fed C57BL/6N mice during 15 weeks of LincIRS2 LNA or Scr LNA (n = 8) treatment. o Fasted and random-fed GTT and ITT in CD-fed 12- and 11-week-old C57BL/6N mice after 6 and 5 weeks of LincIRS2 or Scr LNA (n = 8) treatment. p Fasted and random-fed glucose and ITT in HFD-fed 12- and 11-week-old C57BL/6N mice after 6 and 5 weeks of LincIRS2 or Scr LNA (n = 8 each) treatment. q, r Representative immunoblot and densitometry of t-AKT and pAKTS473 in p CD- or r HFD-fed C57BL/6N mice after 15 weeks of LincIRS2 or Scr LNA treatment injected with 2.5 µl g−1 BW NaCl 0.9% or 0.1 U/mouse insulin (n = 3). Bar graphs represent mean ± s.e.m. with all data plotted and statistical differences calculated using (ai), l unpaired, two-tailed Student’s t test. q One-way ANOVA or mp two-way ANOVA with Bonferroni post correction for multiple testing. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.

We next asked if, like MAFG, LincIRS2 is involved in hepatic glucose control. LNA-mediated LincIRS2 knockdown in primary hepatocytes was highly efficient under basal, FD, GCG, and insulin-stimulated conditions (Fig. 5g, h). Crucially, LincIRS2-deficient primary hepatocytes did not exhibit suppression of Pck1 expression upon insulin stimulation (Fig. 5i). These data suggested in vitro insulin resistance upon LincIRS2 silencing as, in contrast to Mafg, LincIRS2 RNAi did not increase Pck1 expression under basal, FD, and GCG stimulation (Fig. 5j, k).

We next investigated the consequences of LincIRS2 knockdown in vivo by performing biweekly injections of LNAs targeting LincIRS2. LincIRS2 silencing reduced 50% of liver and kidney LincIRS2 in lean mice (Fig. 5l), and again, we observed no differences in BW between genotypes in CD- or HFD-fed mice (Fig. 5m, n). LincIRS2 LNA-treated lean mice were normoglycemic (Fig. 5o, left), yet mildly insulin resistant (Fig. 5o, right), whereas LincIRS2 LNA-treated obese mice were unaffected, presumably due to preestablished insulin resistance in these animals (Fig. 5p). In line with this, LincIRS2 RNAi reduced insulin-mediated AKT/PKB phosphorylation of serine 473 in livers from lean (Fig. 5q) but not obese (Fig. 5r) mice in the absence of changes to glucose tolerance (Supplementary Fig. 4b) or hepatic lipid accumulation (Supplementary Fig. 4c). RNA-Seq analysis of LincIRS2 LNA-treated livers confirmed the deterioration of metabolism at the transcriptome-wide level as DGE in lincRS2-deficient lean mice revealed similar gene sets affected as in Scr LNA-treated obese mice (Supplementary Fig. 4d, Supplementary Data 7 and 9).

Knockout of LincIRS2 causes elevated glucose levels in mice

Given the mild deterioration of metabolism upon transient LincIRS2 knockdown in LNA-treated mice, we wanted to address the role of LincIRS2 in glucose metabolism using a genetic model. For this, we employed CRISPR/Cas9 genome editing in order to target a 418-bp region encompassing exon 1 and the putative promoter region (marked by H3K4me3 and H3K9Ac, Supplementary Fig. 4f–i) of LincIRS2. Sanger sequencing of the LincIRS2 allele confirmed a 426-bp deletion, together with a 5-bp insertion of the desired genomic LincIRS2 locus (Supplementary Fig. 4j). Homozygous C57BL/6N-LincIRS2em/Cecad knockout mice (termed LincIRS2∆/∆) exhibited complete absence of LincIRS2 expression (Supplementary Fig. 4k), were born at Mendelian frequencies, and exhibited no developmental or behavioral abnormalities, changes in BW, energy expenditure, or substrate mobilization (Supplementary Fig. 4l–n). Lean LincIRS2∆/∆ mutants exhibited elevated blood glucose levels under fasted and fed conditions (Fig. 6a) and glucose levels remained high after insulin (Fig. 6b) or glucose challenge (Fig. 6c). Interestingly, LincIRS2∆/∆ mice showed elevated gluconeogenic G6pc, Pck1, and Foxo1 (Fig. 6d), oxidative Acyl-CoA Oxidase 1 (Acox1) and Carnitine Palmitoyltransferase 1A (Cpt1a, Fig. 6e), insulin receptor substrate (Irs) 1 and 2 (Fig. 6f), and Slc2a1-2 glucose transporter expression (Fig. 6g), suggesting altered transcriptional regulation of these enzymes underlying the elevated glycemia in these mice.

Fig. 6: CRISPR-mediated deletion of LincIRS2 impairs glucose metabolism in lean mice.

a Fasted and random-fed glucose in CD-fed 12-week-old LincIRS2∆/∆ mutant mice (n = 8) vs. wild-type littermates (n = 11). b ITT in 11-week-old and c GTT in 12-week-old, CD-fed LincIRS2∆/∆ (n = 8) vs. Control mice (n = 11). dg qPCR of d gluconeogenic e oxidative f IR signaling and g glucose transporter expression in 8–15-week-old LincIRS2∆/∆ vs. LincIRS2∆/wt (n = 8) mice. h Hepatic qPCR and immunoblot (n = 4) from 18- to 20-week-old C57BL/6N 10 weeks after i.v. injection with 1× 10E11 genomic copies/animal encoding AAV8-CTRL or AAV8-MAFG. Fasted i and random-fed j glucose. k, l GTT and m, n ITT in CD-fed 18–20-week-old LincIRS2∆/∆ vs. C57BL/6N Controls (n = 12 each) with half of mice in each genotype (n = 6) i.v. injected with 1× 10E11 genomic copies/animal AAV8-CTRL or AAV8-MAFG. o Illustration of MAFG and LincIRS2 in regulation of hepatic glucose metabolism. Bar graphs represent mean ± s.e.m. with all data plotted and statistical differences were calculated using a, dh unpaired, two-tailed Student’s t test. i, j One-way ANOVA with Bonferroni post correction for multiple testing. b, c, kn Two-way ANOVA with Bonferroni post correction for multiple testing. Superscripts depict group comparisons for post analysis (a–f = comparison vs. columns 1–6) *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.

Finally, and to test if LincIRS2 synergized with MAFG function, we overexpressed Mafg using adeno-associated viruses in C57BL/6 and LincIRS2∆/∆ mice. Adeno-associated virus serotype 8 (AAV8)-mediated overexpression (AAV8-MAFG) increased Mafg mRNA and protein (Fig. 6h), but did not alter fasted or random-fed glucose levels (Fig. 6i, j). Intriguingly though, driving Mafg, alone or coupled to LincIRS2 loss, again caused glucose intolerance to similar extents, arguing for synergistic roles of MAFG overexpression and LincIRS2 deletion (Fig. 6k, l) in glucose metabolism. Conversely, delivery of Mafg alone caused insulin resistance (Fig. 6m), but not when coupled to LincIRS2 loss (Fig. 6n), suggesting independent roles in regulating insulin sensitivity. Taken together, our data identified a transcriptional pathway where MAFG acts as a repressor of metabolically regulated hepatic lncRNAs. We identified a specific MAFG-repressed lncRNA, LincIRS2, that contributed to glucose homeostasis and insulin-mediated suppression of hepatic glucose production in mice. Either genetic LincIRS2 deletion or LincIRS2 RNAi in vivo impaired glucose metabolism, albeit to different degrees, and presumably, via different molecular mechanisms.

Collectively, we demonstrated a hitherto unknown role for MAFG in serving as intracellular sensor of systemic nutrient states, where changes in MAFG signal transduction are coupled to alteration of hepatic glucose homeostasis. Further, increased MAFG signaling partly explained the initial observation of opposite regulation of lncRNAs vs. mRNAs during DIO and T2D. One molecular effector mechanism of how excessive MAFG activity during DIO translated into glucose alterations is repression of liver-selective LincIRS2, which itself integrated anabolic and catabolic metabolites and TF responses (illustrated in Fig. 6o). In vivo genome editing and RNAi against LincIRS2 revealed roles for this MAFG-lncRNA axis in ensuring hepatic glucose handling and maintenance of insulin sensitivity.


Our results establish the TF MAFG as a regulator of energy-rich nutrient states in the liver, both in postprandial responses and during metabolic diseases like obesity and type 2 diabetes. Mechanistically, we demonstrated that MAFG controls a specific set of obesity-associated hepatic lncRNAs that are repressed in obese livers, in addition to the known function of MAFG in bile acid regulation40.

Our results show little evidence for co-regulation with only four disease-associated syntenically conserved lncRNAs in human and mouse livers (Supplementary Data 13) when performing comparative lncRNA analyses using PLAR55. This may be due to (1) misannotation of gene biotypes, as not every lncRNA transcript truly represents a noncoding transcript, evidenced, e.g., by the high protein-coding potential of lncRNA Gm10319, (2) due to the fact that lncRNA conservation at the structural and function level is hard to address computationally56, or (3) conservation of disease-associated lncRNAs simply is low as shown in other cell types57. Another caveat lies in the low number of obesity-associated lncRNAs we identified here. This limits the statistical power of TF motif enrichment algorithms. However, other in silico studies came to similar conclusions in terms of smMAF TFBS enrichment in long noncoding RNA promoters31,32,35.

In our study, we found that MAFG signaling activity was reduced during fasting, which has not been previously reported. We then analyzed both MAFG and its known interaction partner NRF1/NFE2L1 during nutrient deprivation and found that, while both Nrf1/Nfe2l1 and Mafg mRNA were mildly induced, NRF1/NFE2L1, but not MAFG protein, was profoundly upregulated during fasting (Supplementary Fig. 5a, b). By reanalyzing publicly available hepatic RNA-Seq data performed in mice with hepatocyte-specific deletion of NRF1/NFE2L158 (GEO ID: GSE103949), we found that NRF1/NFE2L1 ablation resulted in strikingly similar expression profiles compared with MAFG overexpression (Supplementary Fig. 5c). These data suggested that the interaction of MAFG with NRF1/NFE2L1 constitutes an important regulatory step during fasting in the liver: recruitment of MAFG into NRF1/NFE2L1:MAFG heterodimer complexes, for instance after fasting-induced increases in NRF1/NFE2L1 protein would, in turn, reduce MAFG target gene expression (as during fasting), consistent with a notion where NRF1/NFE2L1 deficiency and MAFG overexpression increase pools of “free” MAFG homodimers with known gene-repressive functions36,37. Although we have not directly addressed the binding partners of MAFG under different nutrient stress conditions, our data suggest that competition between NRF1/NFE2L1 and MAFG is important for liver physiology: as found for MAFG gain of function40, NRF1/NFE2L1 inactivation in the liver downregulated genes involved in cholesterol to bile acid conversion, e.g., Cyp7b158. This suggests that, whereas NRF1/NFE2L1 suppresses inflammation and promotes bile acid synthesis40, MAFG overexpression induces hepatic inflammation (Fig. 5k) and suppresses bile acid conversion40, in addition to suppressing key regulatory lncRNAs such as LincIRS2 (Supplementary Fig. 5d, e and Supplementary Data 11). Whether the MAFG-dependent regulation of lncRNAs (e.g., LincIRS2), or the MAFG-dependent regulation of coding mRNAs are key in mediating the MAFG-dependent changes in energy-rich nutrient states remains to be determined. However, our data suggest that the MAFG-dependent regulation of coding and noncoding mRNAs is complimentary, suggesting that both are required to elicit the full response.

Ultimately, our findings could also indicate that MAFG pathway inhibition, or NRF1/NFE2L1 activation, could generally favor energy-preserving processes where transcription of long noncoding RNAs does not require subsequent mRNA translation. Conversely, postprandial or obesity-associated activation of MAFG would trigger energy-demanding, anaplerotic processes like an increase in cellular proliferation and protein synthesis as a consequence of mTOR-4E-BP1 pathway activation.


Animal care and research diets

All animals were maintained on a C57BL/6N background, housed in groups of 3–5 animals per cage at 22–24 °C on a constant 12-h light/dark cycle in a SPF-controlled facility with regular testing for pathogens. Care of animals and all mouse research was approved by and we adhered to institutional and animal-care committee ethical guidelines from local (Bezirksregierung Köln) or regional (Tierschutzkommission accession no. §15 TSchG of the Landesamt for Natur, Umwelt und Verbraucherschutz (LANUV) North-Rhine Westphalia, Germany, internal reference no. Az-84-02.04-2016.A460) authorities. Upon weaning, mice were fed standard rodent chow (Teklad Global Rodent T.2018.R12; Harlan). For experiments involving controlled feeding paradigms, animals were allowed AL access to CD (D12450B* mod LS; Sniff) containing 62 kJ% carbohydrates, 27 kJ% protein, and 11 kJ% fat and drinking water. DIO was achieved by feeding a HFD (D12492 (I) mod; Sniff) containing 22 kJ% carbohydrates, 24k J% protein, and 54 kJ% fat from starting at 6–7 weeks of age. Experimental mice were exposed to specific diets for 10–12 weeks and 17–18 weeks old at sacrifice unless described otherwise. Six-month-old LIRKO59 (Albumin-Cre+/−, IRflox/flox) and littermate control (Albumin-Cre−/−, IRflox/flox) animals were used.

RNA isolation and total RNA-Sequencing

Liver samples used for total RNA-Sequencing (RNA-Seq) were from male, 36-week-old C57BL/6N mice exposed to CD (n = 3) or HFD (n = 3) feeding for 30 weeks, starting at 6 weeks of age. RNA-Seq of C57BL/6N liver mice exposed to different nutrient states was performed in liver samples from male, 17-week-old, lean AL (n = 4), AL followed by 16 h of fasting (FA, n = 4), and FA followed by 6 h of RF (n = 4) mice. For RNA-Seq from a genetic model of obesity and T2D, male BKS.Cg-Dock7m+/+; LepRdb/J (db/db) mice and BKS.Cg-Dock7m+/+ (misty/misty) control mice were purchased from Jackson Laboratory or Janvier Labs. We used misty mice as control animals, as they are the littermates of db/db mice generated on the BKS.Cg-Dock7m+/+ LepRdb/J background (n = 4 mice per genotype). Tissues were collected at 10 weeks of age. RNA-Seq was also performed in lthe iver from mice treated with adenoviruses expressing Mafg cDNA (Ad-MAFG, n = 3) or empty vector (Ad-CMV, n = 3) under control of mouse cytomegalovirus (CMV) promoter40. Briefly, cDNAs for mouse MAFG, were cloned from whole-liver cDNA into pAdTrack CMV plasmids. For animal experiments, 1× 10E9 plaque-forming units were used, and tissues were collected after 5–7 days of viral treatment. RNA-Seq was also performed in the liver from 21-week-old male mice exposed to CD or HFD and injected 15 weeks with control (Scr), Mafg, or LincIRS2 LNA (n = 4 per genotype and diet). Human liver samples used for RNA-Seq were obtained from male individuals that were with lean and nondiabetic (n = 4; age 60.75 ± 9, body mass index (BMI) 23.52 ± 1.36, Patient IDs: 11_ 0381WSC, 13_1651HRU, 135_1311WER, and 155_3141AZE), obese and nondiabetic (n = 4; age 38.5 ± 13.42, BMI 51 ± 2.98, Patient IDs: 31_0311RAN, 32_0861NSC, 37_1941JRO, and 98_0121STR), overweight, diabetic (n = 2; age 75.5 ± 0.7, BMI 26 ± 1.41, Patient IDs: 54_0951LZU, 56_1781DGR, 95_1701GBO, and 152_3011FHE), and obese and diabetic (n = 2; age 44 ± 2.8, BMI 48.25 ± 20.85). All patients gave written informed consent, and approval of the ethics committee of the University of Ulm was obtained29. Library preparation and sequencing was performed at (1) Max-Planck Genome-Centre (MP-GC, HFD vs. CD cohort) or (2) the Cologne Center for Genomics (CCG), Germany (others). Following quality checks, 1 µg of total liver RNA of each sample was (1) depleted for rRNA using NEBNext® rRNA depletion Kit (human/mouse/rat). Library preparation was performed with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) or (2) depleted for cytoplasmic and mitochondrial rRNA with Ribo-Zero Gold (LNA Cohort) or Ribo-Zero and strand-specific library preparation performed using TruSeq RNA Gold Kit from Illumina. All libraries were sequenced on (1) HiSeq2500 in 2 × 100-bp PE or (2) HiSeq 4000 instruments in 2 × 75 PE sequencing mode.

RNA-Sequencing data processing

  1. (1)

    Mouse RNA-Seq was processed utilizing the GRCm38 assembly of the mouse genome as gene sets from Ensembl release 9060. Biotype and gene features were added manually using Ensembl Biomart. The pipeline consists of six steps: (i) barcode and adapter removal using flexbar 3.4.061, (ii) computational rRNA depletion by filtering reads that map to known rRNAs in mice using Bowtie2 2.2.962, (iii) alignment of non-rRNA reads to the mm10 reference genome using STAR 2.6.0c63, (iv) transcript assembly using cufflinks followed by (v) cuffmerge, and (vi) cuffdiff performs DGE analysis between experimental conditions via Cufflinks suite 2.2.164.

  2. (2)

    Human RNA-Seq data were analyzed using QuickNGS65, version 1.2.2, based on Ensembl release 82. In brief, reads were mapped to GRCm38 assembly of the human genome using Tophat266, version 2.0.10, and reassembled with Cufflinks, version 2.1.1. DGE was analyzed using the DESeq267, version 1.10.1. The results were uploaded to the QuickNGS database and combined with multiple annotations using the biomaRt package version 2.16.0.

Gene overlap analysis

Overlap of regulated gene sets were performed with “venneuler” package for R using genes defined under (1) in the paragraph above. The p value of the overlap was calculated using hypergeometric distribution

$$p = \mathop {\sum }\limits_{i = k}^{\min \left( {m,n} \right)} \frac{{\left( {\begin{array}{*{20}{c}} m \\ i \end{array}} \right)\left( {\begin{array}{*{20}{c}} {N - m} \\ {n - i} \end{array}} \right)}}{{\left( {\begin{array}{*{20}{c}} N \\ n \end{array}} \right)}}$$

where N is the total number of testable genes, m is regulated genes in study A, n is regulated genes in study B, and k is genes overlapping studies A and B.

Ingenuity pathway analysis

IR, FOXO1, and SREBP1C/SREBF1 were loaded as seed gene nodes into IPA and expanded into TF-dependent networks by using the “grow” option with molecular interactions limited to “direct” and “downstream” effects. These manually curated pathways were overlaid with DGE information from RNA-Seq in HFD- vs. CD-fed C57BL/6N mice.

Promoter analyses and motif enrichment analysis

  1. (1)

    For enrichment analyses of known TFBS using AME33 functionality of MEME34, RNA-Seq data of livers from mice on CD or HFD feeding were aligned to mm10 using STAR63, and transcriptome assembly and DGE analyses performed using CuffLinks/CuffDiff68, respectively. Significantly altered mRNAs and lncRNAs were defined by p value ≤ 0.1 and L2R > 0 or <0. lncRNAs and mRNAs were defined by filtering for biotypes “bidirectional_promoter_lncRNA”, “lincRNA”, “sense_intronic”, “sense_overlapping”, “antisense”, and “protein-coding” using annotations from Ensembl Biomart Version 90. Promoter sequences (defined as −800/+100 bp from TSS) of each biotype class were extracted and enriched TFBS motifs identified using commands “ame–verbose 1–oc–bgformat 1–scoring avg–method ranksum–pvalue-report-threshold 0.05 db/JASPAR/JASPAR2018_CORE_vertebrates_non-redundant.meme db/MOUSE/uniprobe_mouse.meme”.

  2. (2)

    For de novo enrichment analyses of TFBS motifs using HOMER, mRNA, and lncRNA promoter sequences from (1) were used as input for HOMER de novo motif analyses (findMotifsGenome.pl) using indicated promoter lists as background. De novo motif analyses were collapsed to motifs known to bind TFs found expressed in at least one condition in the RNA-Seq data (CD/HFD), using the curated motif list from the IMAGE pipeline69.

Chromatin-immunoprecipitation (ChIP) sequencing analysis

Previously published public BLRP ChIP-seq data from livers of mice transduced with adenoviruses overexpressing BLRP-MAFG fusion protein (GEO ID: GSE77559) were used as proxy for MAFG cistromes in the liver. The ChIP-seq data were aligned to mm10 genome using STAR63, and BLRP-MAFG peaks were called in each replicate (n = 2) using findPeaks function in HOMER38. The peak lists were merged using mergePeaks function in HOMER and only peaks called in both replicates were retained for downstream analyses.

In vivo RNAi using LNAs

RNA-interference (RNAi)-mediated silencing of Mafg and LincIRS2 was performed using custom-made LNA GapmeRs that were designed and synthesized by Exiqon, Denmark to target murine Mafg and LincIRS2 (Mafg, LincIRS2 LNA). A negative control with no homology to known mouse transcripts was used as control LNA. Four-week-old C57BL/6N male mice were obtained from Charles River and acclimated for 2 weeks, followed by intravenous (i.v.) injection of 10 mg kg−1 BW LNA dissolved in 0.9% NaCl once every other week for 15 weeks starting from 6 weeks of age. One week after the start of injection series, mice were placed on CD or HFD for 14 weeks. Insulin and glucose tolerance test (ITTs and GTTs) were performed at 6 and 7 weeks of diet, respectively.

In vivo RNAi using antisense oligonucleotides (ASO)

Six- to seven-week-old male C57BL/6N mice or Maff/Mafk-null (double knockout, DKO) mice were injected weekly with 5 mg kg−1 Gal-Nac-conjugated Mafg or Control ASO at a concentration of 1 mg/mL dissolved in 1× phosphate-buffered saline (PBS), for 20 weeks. Two weeks after the first injection, mice were placed on HFD for 18 weeks. ITT was performed at 9 weeks on diet, and GTT was performed at 10 and 16 weeks on diet.

Generation of LincIRS2∆/∆ knockout mice using CRISPR/Cas9

Generation of a mouse model for genetic LincIRS2 deficiency (LincIRS2∆/∆) was performed using CRISPR/Cas9 genome editing where a 426-bp deletion (chr8: 10,899,861–10,900,279) and a 5-bp insertion, encompassing the presumed promoter region (defined by occurrence of histone 3 lysine 4 trimethylation (H3K4me3) near the LincIRS2 TSS in murine liver, based on NIH Epigenomics Roadmap information70) and exon 1 of LincIRS2 (GRCm38/mm10) were excised. To this end, guide RNAs (gRNAs, sequences provided in Supplementary Data 10) were designed using CRISPOR71 and gRNAs with highest specificity scores (≥50) chosen, yet gRNAs with predicted off-target effects for 1 or 2 occurring mismatches excluded. After gRNA identification, target-specific CRISPR RNAs (crRNAs) were incubated with generic transactivating crRNAs (tracrRNAs, Integrated DNA Technologies) to form the active gRNA. Next, annealed gRNAs were incubated with Cas9 proteins to obtain functional ribonucleoprotein complexes. Pronuclear injections of CRISPR/Cas9 complexes into fertilized oocytes of C57BL/6N females were performed at the CECAD in vivo Research Facility72. Briefly, we employed pronuclear injection of CRISPR/Cas9 components into C57BL/6NRj zygotes, which were then implanted into pseudopregnant RjHan:NMRI females. Synthetic target-specific crRNA and sequence-independent tracrRNA molecules were purchased from commercial distributors. Shortly before injection, crRNAs and tracrRNAs were co-incubated with recombinant Cas9 proteins, and to enhance the efficacy of CRISPR/Cas9 genome editing, we added Cas9-encoding mRNA to the injection mix. Healthy, CRISPR/Cas9-injected oocytes were subsequently implanted into oviducts of pseudopregnant 0.5 postcoital foster females. Finally, offspring born from implanted oocytes was analyzed via DNA sequencing, and in the case of successful CRISPR/Cas9-mediated LincIRS2 knockout, bred to C57BL/6N mice to create C57BL/6-LincIRS2em1Cecad transgenic mouse lines. Genotyping primers are available in Supplementary Data 10. For each of the two gRNAs, the top five sites of predicted off-target Cas9 activity were investigated using Sanger Sequencing, and no changes to the GRCm38/mm10 genomic sequence were detected (Supplementary Data 14).

Adeno-associated virus (AAV)-mediated MAFG overexpression

PscAAV8-MAFG-HA encoding plasmids were produced by replacing luciferase (Luc) coding sequence in pscTTR-luc by the respective sequences. AAV8 was produced in HEK293 cells using the triple-transfection method. Specifically, the AAV helper plasmid pXR8, the adenoviral helper plasmid pXX6, and the vector plasmid (pscTTR-luc, pscAAV-MAFG-HA) were transfected in 1:1:1 molar ratio using CaP method. Forty-eight hours post transfection, cells were harvested and lysed. Following benzonase treatment and a low-speed centrifugation preclearing step, preparation was purified by iodixanol step gradient purification. Genomic titer was determined by qPCR using ITR-specific primers. C57BL/6N and LincIRS2∆/∆ male mice were 8–10 weeks of age and i.v. injected with 10E11 vector particles/animal of genomic copies for AAV control (encoding Luc) or AAV-MAFG. Glucose and insulin tolerance test were performed at 8 and 9 weeks after injection.

Isolation of primary hepatocytes and liver fractionation

Mouse primary hepatocytes were isolated from C57BL/6N mice livers by a classical two-step collagenase perfusion method73 with minor modifications as follows: mice were subjected to experiments around 8–12 weeks of age. Under anesthesia, mice were perfused via the portal vein with 50 ml of perfusion medium, followed by digestion with 50 ml of collagenase medium. After liver perfusion, dissociated cells from the liver were filtered through a 70-µm cell filter (BD Falcon) into DMEM, low glucose (Gibco) supplemented with 10% FBS and 1% penicillin–streptomycin (P/S), and centrifuged twice at 50g for 3 min to recover the pellet and the supernatant. Hepatocytes were obtained by resuspending the pellet in 30% Percoll and centrifuging at 150g for 7 min. For obtaining non-hepatocyte (non-parenchymal) cell fractions, the supernatant was centrifuged at 350g, and the resulting cell pellets centrifuged on a 20% (w/vol) Histodenz (Sigma) gradient. For fractionation analyses, both hepatocytes and non-parenchymal fractions were frozen immediately after isolation. For experiments in which hepatocytes were transfected and/or stimulated, cells were attached to six-well collagen I-coated plates (Costar) for 2 h and were grown in P/S-supplemented DMEM, low glucose (Gibco) without FBS overnight; they were then subjected to experimental procedures 24 h after isolation. The media were as follows: Perfusion medium, HBSS (Gibco) without magnesium or calcium and supplemented with 0.5 mM EGTA; collagenase medium, DMEM, low glucose (Gibco) supplemented with 15 mM HEPES and 100 collagen digestion units (CDU) ml−1 of collagenase, type IV (Worthington); 90% concentrated Percoll, 100% Percoll (Amersham) diluted with 10× HBSS (Gibco).

Subcellular fractionation of primary hepatocytes

Subcellular fractions were obtained using freshly isolated primary hepatocytes using Nuclei Isolation Kits: Nuclei EZ Prep according to the manufacturer’s (Sigma Aldrich) protocol. Briefly, 5 × 106 cells were centrifuged (5 min, 300g, 4 °C) and supernatants were discarded, whereas cell pellets were resuspended in 10 ml of PBS and centrifuged again (5 min, 300g, 4 °C). After removal of supernatants, 4 ml of ice-cold Nuclei EZ Lysis Buffer was added to cell pellets and the reaction tube vortexed briefly and kept on ice for 5 min. Following centrifugation (5 min, 300g, 4 °C), supernatants were kept for later analysis (cytoplasmic fraction), while pellets were resuspended in 4 ml of ice-cold Nuclei EZ Lysis Buffer, vortexed briefly, and kept on ice for 5 min. After a final centrifugation (5 min, 300g, 4 °C) supernatants were discarded and nuclei pellets resuspended in 200 µl of ice-cold Nuclei EZ Storage Buffer (nuclear fraction). If not directly processed for RNA isolation, cytoplasmic and nuclear fractions were stored at −80 °C.

LNA-mediated gene knockdown of primary hepatocytes

Primary hepatocytes from C57BL/6N mice were cultured for 24 h at 37 °C and 5% CO2 before transfection of control (Scr), Mafg, and LincIRS2 (4833411C07Rik) LNAs (sequences provided in Supplementary Data 10). Lipofectamine RNAiMax was diluted 1:16 in DMEM. For a final concentration of 100 nM, the respective LNAs (stock 10 µM) were diluted 1:100 in DMEM. Both solutions were incubated for 5 min at room temperature. LNA and Lipofectamine solutions were mixed at equal volumes and incubated for 5 min. Cells were washed with prewarmed 1× PBS and 1.6 ml of DMEM without FBS added to each well. Four-hundred microliters of LNA/Lipofectamine mix was added and cells incubated for 24 h at 37 °C and 5% CO2 before changing the medium. For in vitro stimulation experiments, primary hepatocytes were washed with prewarmed 1× PBS and fresh medium added. Cells were stimulated with 100 nM Insulin (Sigma), Glucagon (0.1 mg ml−1, Sigma), 10 µM Forskolin plus 100 nM Dexamethasone (FD), or FD plus 100 nM Insulin (FDI), and incubated for 10 min, 6 h, or 24 h at 37 °C and 5% CO2, before harvesting cells for further experimental analysis.

Quantification of glucose production

After 24 h of transfection, primary hepatocytes were washed with warm 1× PBS, and glucose-free DMEM (Gibco) supplemented with 100 mM sodium pyruvate was added in order to study cell-intrinsical glucose production. Stimulation with Fsk/Dex (10 µM/100 nM) with/without 100 nM insulin was performed for 24 h at 37 °C and 5% CO2. Supernatant medium of the cells was collected to measure endogenous glucose production with Glucose-Glo Assay Kits (Promega) following the manufacturer’s instructions.

Adenoviral overexpression of metabolic TF in hepatocytes

Adenoviruses encoding YFP (Addgene plasmid #15302), constitutively active mouse FOXO1 (caFOXO1, Addgene plasmid #17547), and constitutively active mouse SREBP1c (caSREBP1C, Addgene plasmid #8883) were subcloned from plasmids (Addgene) into the adenoviral vector system pAd/CMV/V5-DEST (Invitrogen). A constitutively active mouse ChREBP lacking the N-terminal low-glucose inhibitory domain was PCR amplified from mouse liver cDNA74 and cloned into pAd/CMV/V5-DEST. Adenoviruses were amplified in HEK293A cells and purified by CsCl gradient centrifugation. Purified viruses were desalted with PD10 columns (GE Healthcare Life Sciences) and were titered with an Adeno-X Rapid Titer Kit (Clontech). Primary hepatocytes were treated with adenoviruses encoding aforementioned TF for indicated times. For in vitro stimulation experiments, cells were exposed to 100 µM cAMP or 200 nM GCG and incubated for 6 h or stimulated with 100 nM Insulin (Sigma) for 16 h, at 37 °C and 5% CO2, before harvesting cells for experimental analysis.

Immunoblot analysis

For protein isolation, primary hepatocytes grown on six-well plates were washed gently one time with ice-cold 1× PBS and excess 1× PBS aspirated. Five-hundred microliters of RIPA lysis buffer (50 mM Tris-HCL, pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.1% sodium deoxycholate, 1% NP-40, 1× protease inhibitor (Sigma), and 1× phosphatase inhibitor (Roche)) was added to each well of the six-well plate and cells were scraped. Protein lysates were transferred to 1.5-ml Eppendorf tubes, snap-frozen in liquid nitrogen, and thawed on ice for three repeated cycles. Tissues were homogenized in 1 ml of RIPA Buffer using FastPrep 24G (MPBio). After 10 min of centrifugation at 12,000g and 4 °C, the supernatant was collected into fresh tubes, and protein concentration was determined using the Bradford colorimetric protein method (Thermo Fisher Scientific). Protein samples were stored at −80 °C. Samples for immunoblotting were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis after being prepared with 4× Laemmli Sample Buffer containing 10% β-mercaptoethanol and heated to 95 °C for 5 min. Subsequently, proteins were transferred to nitrocellulose membrane and blocked with Western Blocking Reagent (Roche). Membranes were incubated with primary antibodies at 4 °C overnight. After incubation with secondary antibodies, blots were developed with Pierce ECL Western Blotting substrate (Thermo Fisher Scientific). All densiometric measurements were performed with ImageJ. Band intensity was normalized to calnexin (CLNX), which served as internal loading control, blotted on the same gel and membrane. Antibodies were purchased from Cell Signaling Technology and raised against pAKTS473 (Catalog No. 9271), t-AKT (Catalog No. 4685), pTORS2448 (Catalog No. 2971), t-mTOR (Catalog No. 2972), p4E-BP1T37/46 (Catalog No. 2855), t-4E-BP1 (Catalog No. 9452), pGSK3BS9 (Catalog No. 9323), t-GSK3B (Catalog No. 9315), and NRF1/NFE2L1 (Catalog No. 8052). MAFG antibody was purchased from GeneTex (GTX 114541). Primary antibodies were used at a 1:1000 dilution ratio in Tween TBS. CLNX antibody was used at a 1:5,000 dilution and purchased from Calbiochem (Catalog No. 208-880).

Glucose, insulin, and PTT

At the time of performing insulin tolerance tests (ITT), mice were 11 weeks of age and either 4 weeks exposed to CD or HFD feeding. ITT was carried out in random-fed mice in the morning. After determining basal blood glucose levels, each animal received 0.75 IU per kg BW of insulin (Insuman Rapid; Sanofi-Aventis). For glucose tolerance tests (GTT), mice were 12 weeks of age and 5 weeks exposed to CD or HFD feeding. After 16 h of fasting and measurement of basal blood glucose levels, animals were i.p. injected with 2 g per kg BW of glucose (20% glucose, Delta select). Blood glucose levels were recorded using an automatic glucose monitor (Contour, Bayer Diabetes Care) at the indicated time points in (1) male Scr vs. Mafg and LincIRS2 LNA-treated mice, (2) Control ASO vs. Mafg ASO, and (3) LincIRS2wt/wt (Control for LincIRS2∆/∆) and LincIRS2∆/∆ mice. In the ASO cohort, animals that showed no decrease of blood glucose after insulin gavage throughout all measurements were excluded from ITT analysis, assuming injection of insulin outside of the peritoneal cavity as required for the assay. The pyruvate tolerance test (PTT) was performed in HFD-fed animals after 6 weeks of HFD feeding with Scr and Mafg LNA injection 5 days before the experiment. For the PTT itself, 4 g of pyruvate was dissolved in 20 ml of 0.9% NaCl and injected 10 µl g−1 BW after 16 h of fasting (final dose 2 g pyruvate kg−1).

Indirect calorimetry (PhenoMaster)

Upon indirect calorimetry measurements, mice of indicated genotypes and diets were 16 weeks of age and 10 weeks exposed to CD or HFD. All measurements were performed using a PhenoMaster System (TSE Systems), which allowed in-cage metabolic and activity monitoring. Three days before analysis, mice were placed alone in training cages, identical to the 7.1-l chambers of the PhenoMaster open-circuit calorimetry system and continued to receive the respective diets (CD and HFD) throughout training and data acquisition. Diets and water were provided AL in the appropriate devices, and food intake measured by the built-in automated instruments. Parameters of indirect calorimetry were measured initially for 96 h at 22 °C and mean values calculated for each time of day.

Tissue collection

At the end of the experimental protocol, LNA-injected mice were randomly assigned to either intravenous (i.v.) injection of 0.9% NaCl or 0.1 U/animal of insulin (Sigma). All mice were sacrificed by carbon dioxide (CO2) asphyxiation. All tissues were washed with 1× PBS, weighed, snap-frozen in liquid nitrogen, and stored at −80 °C.

Enzyme-linked immunosorbent assay

Fibroblast Growth Factor 21 Mouse (FGF21) ELISA was obtained from Biovendor RD291108200R (Czech Republic), and performed using serum from CD and HFD-fed mice, Scr vs. Mafg LNA treated following the manufacturer’s instructions.

Histological staining

For immunostaining, liver tissues were stored in PFA 4% for 24 h and then 70% ethanol for histological analysis. Transveral cryosections from paraffin-embedded liver were prepared, fixed, and stained by hematoxylin and eosin (H/E).

RNA isolation and quantitative RT-PCR (qPCR) analysis

Total RNA was isolated from primary hepatocytes and whole tissues using peqGOLD TriFast (PEQLAB Biotechnologie). mRNA was reverse transcribed into complementary DNA using High Capacity cDNA reverse transcription kit (Applied Biosystems). Abundances of mRNAs were quantified by TaqMan Assay on Demand Kits (Applied Biosystems) according to the manufacturer’s protocol if not indicated otherwise. Abundances of lncRNAs were quantified using SYBR methodology using Select Master Mixes (Thermo Fisher). The relative abundance of mRNAs was calculated using comparative methods (2−∆∆CT) according to ABI Relative Quantification Methods. Transcript levels were normalized to hypoxanthine phosphoribosyltransferase 1 (Hprt1) or glyceraldehyde-3-phosphate dehydrogenase (Gapdh) expression; Hprt and Gapdh abundances remained unaffected across experimental conditions. SYBR primer sequences are provided in Supplementary Data 10.

Reporting summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

Data availability

The source data underlying Fig. 1c–i, Fig. 2b, d, e, g, i, Fig. 3a–h, Fig. 4a–j, l–r, Fig. 5a–r, Fig. a–n, and Supplementary Fig. 1d–f, h–I, Fig. 2c–k, Fig. 3a–g, i–j, Fig. 4b, k–n, Fig. 5a–b, e are provided as Source Data file. Data from RNA-Seq are available under GEO Super Series GSE121346.


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We thank Brigitte Hampel and Pia Scholl for H/E stainings, Jens Alber for technical support with indirect calorimetry measurements and mouse handling, and Lisa Czaja for help with RNA-Seq. MPJ was supported by an Albert Renold Travel Fellowship from the European Association for the Study of Diabetes (EASD). H.T. was awarded with a Short-Term Fellowship from the European Molecular Biology Organization (EMBO). J.W.K., M.O. and E.S. were supported by the Emmy-Noether Program of the Deutsche Forschungsgemeinschaft (DFG, KO4728/1.1). J.W.K., B.D.L. and C.K. received funding from the University of Southern Denmark (SDU) and Danish Diabetes Academy (DDA), which is funded by the Novo Nordisk Foundation (NNF). J.W.K., R.L., E.F.R., M.P.J. and P.K. received support from European Research Council Starting (Grant No. 675014). E.S. was supported by Evangelisches Studienwerk Villigst e.V. S.K. appreciates a PhD stipend from the German Academic Exchange Service (DAAD). N.R.H. was awarded with a PhD stipend from the Cologne Graduate School for Ageing (CGA). R.N.K. and D.F.D. received support from NIH Grants RO1 DK103215, RO1 DK67536, RO1 117639, and DK P036836. J.C.L. is supported by UPLIFT: UCLA Postdocs’ Longitudinal Investment in Faculty (Award # K12 GM106996). L.Y. and H.C. were supported by intramural NIH research funding grants (references 1ZIAHL006103 and 1ZIAHL006159). L.Y. was supported by NIH grant K22HL139921. T.Q. de A.V. is funded by NIH grants DK112119 and HL122677 and P.A.E. and T.Q. de A.V. are funded by DK118064.

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M.P.J., N.R.H., J.C.L., E.S., N.M., R.L., I.D., S.K., M.O., H.T., M.A., H.B., L.Y. and E.F.R. performed the experiments. R.S. performed training in CRISPR/Cas9 editing. M.H., L.S., J.H. and U.K. provided human liver biopsies and information about patient characteristics and helped with data analysis. S.E.T. and B.Z. performed CRISP/Cas9 gene targeting. P.F., P.K., C.D., C.A.K., B.D.L. and I.U. helped with RNA-Seq and ChiP-Seq analysis, data integration, analysis of mouse/human lncRNA synteny, and discussions. H.C. conceived and analyzed LincIRS2 expression after adenoviral TF transduction. D.D.J. analyzed LincIRS2 expression in LIRKO mice. P.A.E. provided discussions and infrastructure. B.Z. and S.T. generated LincIRS2-knockout mice. R.G.L. provided Mafg ASOs. M.Y. provided Maff/Mafk-knockout mice. M.P.J., N.R.H., H.B., M.A., H.C., R.N.K., H.B., I.U., E.F.R., T.Q.A.V. and J.W.K. conceived the experiments.

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Correspondence to Thomas Q. de Aguiar Vallim or Jan-Wilhelm Kornfeld.

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Pradas-Juni, M., Hansmeier, N.R., Link, J.C. et al. A MAFG-lncRNA axis links systemic nutrient abundance to hepatic glucose metabolism. Nat Commun 11, 644 (2020). https://doi.org/10.1038/s41467-020-14323-y

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